pycom15g25840

Belongs to the glycosyl hydrolase 17 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0009502
Physical Location & Seq
Reverse (-)
20508439 .. 20509073
635 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 540 bp
ATGCTCTCCCCGGAGGCACAAACAGCCTCTCCACCACCACCACAAATAGCCTCGCCACCAGACATGTCTTTCTCCTCAGCGCCAGAAAACCCTCCTTTTGTTGATGAATCCCCTCTTTCTCCGCCTTCCCCTGCCACATTTCCACCCTGCCGCAATCCGATTCCGACACATGTGCCGCCTCCTGCCCCTGCCCCTCAGATGGCGGTGCACAAGCTGTGGTGTGTGGCCAAGCCTACCGTTCCTTCAGACACTCTCCAGGTGGCGATGGATTATGCCTGCGGAGACGGCGATGCTGACTGCAAGGAGATCATGCCGGATGGCAACTGCTACAACCCTGATTCTGTTGTGGCACATGCTTCCTATGCCTTCAACAGCTACTGGCAGAAGAACAAGAGGACCGGAGGGACTTGCAACTTCGATGGCACTGCAATGCTGATCAACAATCGATCCAAGTTATCTTCAATGTCGTTTCGTTCTCACTTGAATGCGTTCGGAGCAGAAACTGAGACTGGTGGCTGTTGTTTTATGATGAGGATTTGA

Protein Analysis

180

Amino Acids

19.18

Weight (kDa)

5.2

Isoelectric Point (pI)

74.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
X8 PF07983 72 - 144 1.1e-24 X8 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 5 cut(s) 122, 151, 176, 203, 279
AclWI GGATC 1 cut(s) 441
AcoI YGGCCR 1 cut(s) 225
AcuI CTGAAG 1 cut(s) 228
AfiI CCNNNNNNNGG 1 cut(s) 199
AflIII ACRYGT 2 cut(s) 63, 169
AgsI TTSAA 3 cut(s) 370, 462, 484
AjnI CCWGG 1 cut(s) 255
AluBI AGCT 2 cut(s) 214, 375
AluI AGCT 2 cut(s) 214, 375
Alw21I GWGCWC 1 cut(s) 210
Alw26I GTCTC 2 cut(s) 276, 500
Alw44I GTGCAC 1 cut(s) 206
AlwI GGATC 1 cut(s) 441
AlwNI CAGNNNCTG 2 cut(s) 378, 503
AoxI GGCC 1 cut(s) 225
ApaLI GTGCAC 1 cut(s) 206
Asp700I GAANNNNTTC 1 cut(s) 488
AspLEI GCGC 1 cut(s) 82
AspS9I GGNCC 1 cut(s) 396
AsuC2I CCSGG 1 cut(s) 11
AvaII GGWCC 1 cut(s) 396
BaeGI GKGCMC 1 cut(s) 210
BalI TGGCCA 1 cut(s) 227
Bbv12I GWGCWC 1 cut(s) 210
BbvCI CCTCAGC 1 cut(s) 76
BccI CCATC 4 cut(s) 193, 259, 311, 413
BceAI ACGGC 1 cut(s) 301
BcgI CGANNNNNNTGC 4 cut(s) 154, 188, 407, 441
BciT130I CCWGG 1 cut(s) 257
BclI TGATCA 1 cut(s) 435
BcnI CCSGG 1 cut(s) 11
BcoDI GTCTC 2 cut(s) 276, 500
BfoI RGCGCY 1 cut(s) 83
BisI GCNGC 2 cut(s) 151, 176
BlsI GCNGC 2 cut(s) 152, 177
Bme1390I CCNGG 2 cut(s) 11, 257
Bme18I GGWCC 1 cut(s) 396
BmgT120I GGNCC 1 cut(s) 396
BmrFI CCNGG 2 cut(s) 11, 257
BmsI GCATC 1 cut(s) 280
BpmI CTGGAG 1 cut(s) 239
Bpu10I CCTNAGC 1 cut(s) 76
BpuMI CCSGG 1 cut(s) 11
Bsa29I ATCGAT 1 cut(s) 445
BsaJI CCNNGG 1 cut(s) 9
BsaWI WCCGGW 1 cut(s) 398
BsaXI ACNNNNNCTCC 2 cut(s) 13, 43
Bsc4I CCNNNNNNNGG 1 cut(s) 199
Bse1I ACTGG 2 cut(s) 383, 514
Bse3DI GCAATG 1 cut(s) 435
BseBI CCWGG 1 cut(s) 257
BseCI ATCGAT 1 cut(s) 445
BseDI CCNNGG 1 cut(s) 9
BseGI GGATG 1 cut(s) 322
BseLI CCNNNNNNNGG 1 cut(s) 199
BseMI GCAATG 1 cut(s) 435
BseMII CTCAG 3 cut(s) 90, 209, 495
BseNI ACTGG 2 cut(s) 383, 514
BseRI GAGGAG 1 cut(s) 64
BseSI GKGCMC 1 cut(s) 210
BshFI GGCC 1 cut(s) 227
BshVI ATCGAT 1 cut(s) 445
BsiHKAI GWGCWC 1 cut(s) 210
BsiSI CCGG 3 cut(s) 11, 314, 399
BslFI GGGAC 1 cut(s) 418
BslI CCNNNNNNNGG 1 cut(s) 199
BsmAI GTCTC 2 cut(s) 276, 500
BsmBI CGTCTC 1 cut(s) 276
BsmFI GGGAC 1 cut(s) 418
BsmI GAATGC 1 cut(s) 490
BsnI GGCC 1 cut(s) 227
Bsp1286I GDGCHC 1 cut(s) 210
Bsp143I GATC 3 cut(s) 306, 435, 446
BspACI CCGC 5 cut(s) 122, 151, 176, 203, 279
BspANI GGCC 1 cut(s) 227
BspCNI CTCAG 3 cut(s) 89, 208, 496
BspDI ATCGAT 1 cut(s) 445
BspPI GGATC 1 cut(s) 441
BsrDI GCAATG 1 cut(s) 435
BsrI ACTGG 2 cut(s) 383, 514
BssECI CCNNGG 1 cut(s) 9
BssMI GATC 3 cut(s) 306, 435, 446
Bst2UI CCWGG 1 cut(s) 257
Bst4CI ACNGT 1 cut(s) 238
BstC8I GCNNGC 1 cut(s) 277
BstDEI CTNAG 3 cut(s) 76, 195, 504
BstF5I GGATG 1 cut(s) 322
BstH2I RGCGCY 1 cut(s) 83
BstHHI GCGC 1 cut(s) 82
BstKTI GATC 3 cut(s) 309, 438, 449
BstMAI GTCTC 2 cut(s) 276, 500
BstMBI GATC 3 cut(s) 306, 435, 446
BstMWI GCNNNNNNNGC 4 cut(s) 23, 285, 362, 494
BstNI CCWGG 1 cut(s) 257
BstNSI RCATGY 3 cut(s) 67, 173, 356
BstSCI CCNGG 2 cut(s) 9, 255
BstSLI GKGCMC 1 cut(s) 210
Bsu15I ATCGAT 1 cut(s) 445
BsuRI GGCC 1 cut(s) 227
BsuTUI ATCGAT 1 cut(s) 445
BtgZI GCGATG 2 cut(s) 278, 303
BtsCI GGATG 1 cut(s) 322
BtsI GCAGTG 1 cut(s) 423
BtsIMutI CAGTG 1 cut(s) 423
Cac8I GCNNGC 1 cut(s) 277
CaiI CAGNNNCTG 2 cut(s) 378, 503
CfoI GCGC 1 cut(s) 82
Cfr13I GGNCC 1 cut(s) 396
ClaI ATCGAT 1 cut(s) 445
CviAII CATG 4 cut(s) 64, 170, 310, 353
CviJI RGCY 7 cut(s) 26, 50, 214, 227, 232, 375, 516
CviKI_1 RGCY 7 cut(s) 26, 50, 214, 227, 232, 375, 516
DdeI CTNAG 3 cut(s) 76, 195, 504
DpnI GATC 3 cut(s) 308, 437, 448
DpnII GATC 3 cut(s) 306, 435, 446
EaeI YGGCCR 1 cut(s) 225
EciI GGCGGA 1 cut(s) 111
Eco47I GGWCC 1 cut(s) 396
Eco57I CTGAAG 1 cut(s) 228
EcoRII CCWGG 1 cut(s) 255
Esp3I CGTCTC 1 cut(s) 276
FaeI CATG 4 cut(s) 67, 173, 313, 356
FaiI YATR 7 cut(s) 65, 171, 273, 311, 354, 363, 527
FaqI GGGAC 1 cut(s) 418
FatI CATG 4 cut(s) 63, 169, 309, 352
FbaI TGATCA 1 cut(s) 435
Fnu4HI GCNGC 2 cut(s) 151, 176
FokI GGATG 1 cut(s) 329
Fsp4HI GCNGC 2 cut(s) 151, 176
GlaI GCGC 1 cut(s) 81
GluI GCNGC 2 cut(s) 151, 176
GsuI CTGGAG 1 cut(s) 239
HaeII RGCGCY 1 cut(s) 83
HaeIII GGCC 1 cut(s) 227
HapII CCGG 3 cut(s) 11, 314, 399
HhaI GCGC 1 cut(s) 82
Hin1II CATG 4 cut(s) 67, 173, 313, 356
Hin6I GCGC 1 cut(s) 80
HinP1I GCGC 1 cut(s) 80
HinfI GANTC 3 cut(s) 107, 160, 338
HpaII CCGG 3 cut(s) 11, 314, 399
Hpy166II GTNNAC 1 cut(s) 208
Hpy188I TCNGA 5 cut(s) 159, 165, 198, 247, 494
Hpy8I GTNNAC 1 cut(s) 208
HpyAV CCTTC 3 cut(s) 135, 252, 376
HpyCH4III ACNGT 1 cut(s) 238
HpyCH4V TGCA 4 cut(s) 208, 300, 411, 428
HpyF10VI GCNNNNNNNGC 4 cut(s) 23, 285, 362, 494
HpyF3I CTNAG 3 cut(s) 76, 195, 504
Hsp92II CATG 4 cut(s) 67, 173, 313, 356
HspAI GCGC 1 cut(s) 80
Ksp22I TGATCA 1 cut(s) 435
Kzo9I GATC 3 cut(s) 306, 435, 446
LmnI GCTCC 1 cut(s) 494
LweI GCATC 1 cut(s) 280
MalI GATC 3 cut(s) 308, 437, 448
MboI GATC 3 cut(s) 306, 435, 446
MboII GAAGA 2 cut(s) 397, 450
MhlI GDGCHC 1 cut(s) 210
MlsI TGGCCA 1 cut(s) 227
MluNI TGGCCA 1 cut(s) 227
MmeI TCCRAC 1 cut(s) 188
Mox20I TGGCCA 1 cut(s) 227
MroXI GAANNNNTTC 1 cut(s) 488
MscI TGGCCA 1 cut(s) 227
MslI CAYNNNNRTG 2 cut(s) 428, 483
Msp20I TGGCCA 1 cut(s) 227
MspI CCGG 3 cut(s) 11, 314, 399
MspR9I CCNGG 2 cut(s) 11, 257
Mva1269I GAATGC 1 cut(s) 490
MvaI CCWGG 1 cut(s) 257
MwoI GCNNNNNNNGC 4 cut(s) 23, 285, 362, 494
NciI CCSGG 1 cut(s) 11
NdeII GATC 3 cut(s) 306, 435, 446
NlaIII CATG 4 cut(s) 67, 173, 313, 356
NspI RCATGY 3 cut(s) 67, 173, 356
PciI ACATGT 2 cut(s) 63, 169
PctI GAATGC 1 cut(s) 490
PdmI GAANNNNTTC 1 cut(s) 488
PfeI GAWTC 3 cut(s) 107, 160, 338
PkrI GCNGC 2 cut(s) 152, 177
PscI ACATGT 2 cut(s) 63, 169
Psp6I CCWGG 1 cut(s) 255
PspGI CCWGG 1 cut(s) 255
PspPI GGNCC 1 cut(s) 396
PstNI CAGNNNCTG 2 cut(s) 378, 503
RseI CAYNNNNRTG 2 cut(s) 428, 483
SatI GCNGC 2 cut(s) 151, 176
Sau3AI GATC 3 cut(s) 306, 435, 446
Sau96I GGNCC 1 cut(s) 396
ScrFI CCNGG 2 cut(s) 11, 257
SduI GDGCHC 1 cut(s) 210
SetI ASST 3 cut(s) 216, 261, 377
SfaNI GCATC 1 cut(s) 280
SinI GGWCC 1 cut(s) 396
SmiMI CAYNNNNRTG 2 cut(s) 428, 483
SsiI CCGC 5 cut(s) 122, 151, 176, 203, 279
StyD4I CCNGG 2 cut(s) 9, 255
TaaI ACNGT 1 cut(s) 238
TaqI TCGA 2 cut(s) 417, 445
TauI GCSGC 2 cut(s) 153, 178
TfiI GAWTC 3 cut(s) 107, 160, 338
TscAI CASTG 1 cut(s) 430
TspDTI ATGAA 1 cut(s) 120
TspRI CASTG 1 cut(s) 430
VneI GTGCAC 1 cut(s) 206
VpaK11BI GGWCC 1 cut(s) 396
XceI RCATGY 3 cut(s) 67, 173, 356
XmnI GAANNNNTTC 1 cut(s) 488
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.