pycom15g29410

ATP synthase 24 kDa subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0010249
Physical Location & Seq
Forward (+)
26262605 .. 26267022
4418 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 723 bp
ATGGCCTTCTCTTCCCGCCTCTTATCCAAATCCAGACAGCTGTGTGGCAATGGAGTCATTCTTCAACAGGATCGCGTTATTCCGGTCCGTTATTTCGCCAAGGAAGCAAGTCGCCCCGTGCTAAAGGGTGATGAGATGCTGAAGAACATCTTCTTGGATGTTAAAAAGAAATTTGAGACAGCTCTTGGTATACTTCGCAAAGAGAAGATCACCATTGACCCAGACGATCCAGCTGCTGTTTCTCAGTACACCAAGGTCATGAAAACCATAAGAGAGAAGGCTGATTTGTTCTCAGAATCCCAGCGCATCAAACACACCATCCAAACCCGAACACAAGATATCCCAGATGCTAGAACATATCTGTTGACATTGAAAGAAATTAGGATCAAGAGAGGTCTCACTGACGAACTTGGTGCTGAGGCTATGATGATGGATGCATTGGAGAAAGTTGAAAAGGAGCTGAAGAAACCACTTCTGAGGAGTGATAAAAAGGGGATGGCTGTTCTCATGGCAGAATTTGAGAAAATAAACCAGAAGCTTGGAATTCGTAAGGAGGACCTACCAAAGTATGAAGAACAGCTAGAACTCAAGAAAGCCAAAGCACAGCTGGAGGAACTTAAGAAGGATGCTCTTGAGGCAATGGAAACTCAGAAGAAACGGGAGGAATTTAAGGATGAGGCAATGGTTGATGTGAAGTCGTTAGACATCCGAAACTTCATCTAA

Protein Analysis

241

Amino Acids

27.78

Weight (kDa)

9.3

Isoelectric Point (pI)

28.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mt_ATP_synt PF15704 33 - 219 5.6e-82 Mitochondrial ATP synthase subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0010249)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G21870 AT2G21870
fragaria_vesca FvH4_1g22890
malus_domestica MD01G1021000.v1.1 MD15G1331100.v1.1
prunus_persica Prupe.6G169300_v2.0.a1
pyrus_communis pycom01g05060 pycom15g29410
rosa_chinensis RchiOBHm_Chr2g0117231
rosa_laevigata RLG00000018337
rosa_multiflora Rmu_co8431947.1_g000001
rosa_roxburghii Rroxscaffold_2G00126620
rosa_rugosa Rorug02G0211500
rosa_samantha Rh2AG267100 Rh2AG268800 Rh2BG279400 Rh2DG274000 Rh2DG275900 Rh2DG293400
rosa_wichuraiana Rw2G021080 Rw2G021130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 190
AccII CGCG 1 cut(s) 75
AciI CCGC 1 cut(s) 16
AclWI GGATC 3 cut(s) 78, 221, 392
AcsI RAATTY 4 cut(s) 170, 515, 543, 665
AcuI CTGAAG 2 cut(s) 161, 482
AfaI GTAC 1 cut(s) 248
AflII CTTAAG 1 cut(s) 617
AgsI TTSAA 3 cut(s) 65, 373, 452
AjuI GAANNNNNNNTTGG 2 cut(s) 137, 169
AluBI AGCT 7 cut(s) 40, 182, 233, 460, 538, 580, 607
AluI AGCT 7 cut(s) 40, 182, 233, 460, 538, 580, 607
Alw26I GTCTC 2 cut(s) 170, 401
AlwI GGATC 3 cut(s) 78, 221, 392
AlwNI CAGNNNCTG 1 cut(s) 236
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 1 cut(s) 233
ApoI RAATTY 4 cut(s) 170, 515, 543, 665
Asp700I GAANNNNTTC 1 cut(s) 149
AspLEI GCGC 1 cut(s) 306
AspS9I GGNCC 2 cut(s) 85, 556
AsuHPI GGTGA 2 cut(s) 140, 202
AvaII GGWCC 2 cut(s) 85, 556
BbvCI CCTCAGC 1 cut(s) 417
BbvI GCAGC 1 cut(s) 220
BccI CCATC 3 cut(s) 326, 424, 490
BcgI CGANNNNNNTGC 4 cut(s) 215, 249, 395, 429
BcoDI GTCTC 2 cut(s) 170, 401
BfaI CTAG 2 cut(s) 351, 581
BfrI CTTAAG 1 cut(s) 617
BisI GCNGC 1 cut(s) 234
BlsI GCNGC 1 cut(s) 235
Bme18I GGWCC 2 cut(s) 85, 556
BmgT120I GGNCC 2 cut(s) 85, 556
BmsI GCATC 5 cut(s) 126, 315, 337, 424, 616
BpmI CTGGAG 1 cut(s) 629
Bpu10I CCTNAGC 1 cut(s) 417
BpuEI CTTGAG 2 cut(s) 572, 653
BsaI GGTCTC 1 cut(s) 401
BsaJI CCNNGG 2 cut(s) 99, 252
BsaWI WCCGGW 1 cut(s) 82
Bse3DI GCAATG 3 cut(s) 55, 645, 687
BseDI CCNNGG 2 cut(s) 99, 252
BseGI GGATG 7 cut(s) 163, 318, 439, 501, 631, 679, 705
BseMI GCAATG 3 cut(s) 55, 645, 687
BseMII CTCAG 5 cut(s) 257, 306, 408, 467, 662
BseRI GAGGAG 1 cut(s) 493
BseXI GCAGC 1 cut(s) 220
BseYI CCCAGC 1 cut(s) 300
Bsh1236I CGCG 1 cut(s) 75
BshFI GGCC 1 cut(s) 5
BsiSI CCGG 1 cut(s) 83
BsmAI GTCTC 2 cut(s) 170, 401
BsnI GGCC 1 cut(s) 5
Bso31I GGTCTC 1 cut(s) 401
Bsp143I GATC 4 cut(s) 70, 207, 226, 384
BspACI CCGC 1 cut(s) 16
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 5 cut(s) 256, 305, 409, 468, 661
BspFNI CGCG 1 cut(s) 75
BspHI TCATGA 1 cut(s) 258
BspPI GGATC 3 cut(s) 78, 221, 392
BspTI CTTAAG 1 cut(s) 617
BspTNI GGTCTC 1 cut(s) 401
BsrDI GCAATG 3 cut(s) 55, 645, 687
BssECI CCNNGG 2 cut(s) 99, 252
BssMI GATC 4 cut(s) 70, 207, 226, 384
BssNAI GTATAC 1 cut(s) 191
BssT1I CCWWGG 2 cut(s) 99, 252
Bst1107I GTATAC 1 cut(s) 191
Bst6I CTCTTC 1 cut(s) 16
BstAFI CTTAAG 1 cut(s) 617
BstDEI CTNAG 5 cut(s) 243, 292, 417, 476, 648
BstF5I GGATG 7 cut(s) 163, 318, 439, 501, 631, 679, 705
BstFNI CGCG 1 cut(s) 75
BstHHI GCGC 1 cut(s) 306
BstKTI GATC 4 cut(s) 73, 210, 229, 387
BstMAI GTCTC 2 cut(s) 170, 401
BstMBI GATC 4 cut(s) 70, 207, 226, 384
BstMWI GCNNNNNNNGC 2 cut(s) 104, 635
BstUI CGCG 1 cut(s) 75
BstV1I GCAGC 1 cut(s) 220
BstXI CCANNNNNNTGG 1 cut(s) 539
BstZ17I GTATAC 1 cut(s) 191
BsuRI GGCC 1 cut(s) 5
BtsCI GGATG 7 cut(s) 163, 318, 439, 501, 631, 679, 705
BtsIMutI CAGTG 1 cut(s) 399
CaiI CAGNNNCTG 1 cut(s) 236
CciI TCATGA 1 cut(s) 258
CfoI GCGC 1 cut(s) 306
Cfr13I GGNCC 2 cut(s) 85, 556
CpoI CGGWCCG 1 cut(s) 85
Csp6I GTAC 1 cut(s) 247
CspI CGGWCCG 1 cut(s) 85
CviAII CATG 2 cut(s) 259, 508
CviQI GTAC 1 cut(s) 247
DdeI CTNAG 5 cut(s) 243, 292, 417, 476, 648
DpnI GATC 4 cut(s) 72, 209, 228, 386
DpnII GATC 4 cut(s) 70, 207, 226, 384
Eam1104I CTCTTC 1 cut(s) 16
EarI CTCTTC 1 cut(s) 16
Eco130I CCWWGG 2 cut(s) 99, 252
Eco31I GGTCTC 1 cut(s) 401
Eco32I GATATC 1 cut(s) 340
Eco47I GGWCC 2 cut(s) 85, 556
Eco57I CTGAAG 2 cut(s) 161, 482
EcoO109I RGGNCCY 1 cut(s) 556
EcoRI GAATTC 1 cut(s) 543
EcoRV GATATC 1 cut(s) 340
EcoT14I CCWWGG 2 cut(s) 99, 252
EcoT22I ATGCAT 1 cut(s) 439
ErhI CCWWGG 2 cut(s) 99, 252
FaeI CATG 2 cut(s) 262, 511
FaiI YATR 7 cut(s) 191, 260, 269, 358, 425, 509, 570
FalI AAGNNNNNCTT 2 cut(s) 134, 166
FatI CATG 2 cut(s) 258, 507
FauI CCCGC 1 cut(s) 23
FblI GTMKAC 1 cut(s) 190
Fnu4HI GCNGC 1 cut(s) 234
FokI GGATG 7 cut(s) 170, 305, 446, 508, 638, 686, 692
Fsp4HI GCNGC 1 cut(s) 234
FspBI CTAG 2 cut(s) 351, 581
GlaI GCGC 1 cut(s) 305
GluI GCNGC 1 cut(s) 234
GsaI CCCAGC 1 cut(s) 304
GsuI CTGGAG 1 cut(s) 629
HaeIII GGCC 1 cut(s) 5
HapII CCGG 1 cut(s) 83
HhaI GCGC 1 cut(s) 306
Hin1II CATG 2 cut(s) 262, 511
Hin6I GCGC 1 cut(s) 304
HinP1I GCGC 1 cut(s) 304
HincII GTYRAC 1 cut(s) 366
HindII GTYRAC 1 cut(s) 366
HindIII AAGCTT 1 cut(s) 536
HinfI GANTC 2 cut(s) 54, 296
HpaII CCGG 1 cut(s) 83
HphI GGTGA 2 cut(s) 140, 202
Hpy166II GTNNAC 3 cut(s) 191, 249, 366
Hpy188I TCNGA 4 cut(s) 295, 477, 651, 710
Hpy188III TCNNGA 5 cut(s) 33, 259, 388, 589, 632
Hpy8I GTNNAC 3 cut(s) 191, 249, 366
HpyAV CCTTC 3 cut(s) 16, 271, 616
HpyCH4V TGCA 1 cut(s) 437
HpyF10VI GCNNNNNNNGC 2 cut(s) 104, 635
HpyF3I CTNAG 5 cut(s) 243, 292, 417, 476, 648
Hsp92II CATG 2 cut(s) 262, 511
HspAI GCGC 1 cut(s) 304
Kzo9I GATC 4 cut(s) 70, 207, 226, 384
LmnI GCTCC 1 cut(s) 457
LpnPI CCDG 9 cut(s) 46, 53, 96, 234, 243, 314, 357, 545, 593
Lsp1109I GCAGC 1 cut(s) 220
LweI GCATC 5 cut(s) 126, 315, 337, 424, 616
MaeI CTAG 2 cut(s) 351, 581
MalI GATC 4 cut(s) 72, 209, 228, 386
MboI GATC 4 cut(s) 70, 207, 226, 384
MboII GAAGA 8 cut(s) 3, 53, 142, 154, 217, 475, 584, 664
MluCI AATT 5 cut(s) 170, 378, 515, 543, 665
MlyI GAGTC 1 cut(s) 63
MnlI CCTC 9 cut(s) 29, 386, 412, 471, 547, 604, 628, 655, 670
Mph1103I ATGCAT 1 cut(s) 439
MroXI GAANNNNTTC 1 cut(s) 149
MseI TTAA 3 cut(s) 162, 618, 669
MspA1I CMGCKG 3 cut(s) 40, 233, 607
MspCI CTTAAG 1 cut(s) 617
MspI CCGG 1 cut(s) 83
MvnI CGCG 1 cut(s) 75
MwoI GCNNNNNNNGC 2 cut(s) 104, 635
NdeII GATC 4 cut(s) 70, 207, 226, 384
NlaIII CATG 2 cut(s) 262, 511
NsiI ATGCAT 1 cut(s) 439
PagI TCATGA 1 cut(s) 258
PdmI GAANNNNTTC 1 cut(s) 149
PfeI GAWTC 1 cut(s) 296
PkrI GCNGC 1 cut(s) 235
PleI GAGTC 1 cut(s) 62
PpsI GAGTC 1 cut(s) 62
PpuMI RGGWCCY 1 cut(s) 556
Psp5II RGGWCCY 1 cut(s) 556
PspFI CCCAGC 1 cut(s) 300
PspPI GGNCC 2 cut(s) 85, 556
PspPPI RGGWCCY 1 cut(s) 556
PstNI CAGNNNCTG 1 cut(s) 236
PvuII CAGCTG 3 cut(s) 40, 233, 607
RsaI GTAC 1 cut(s) 248
RsaNI GTAC 1 cut(s) 247
Rsr2I CGGWCCG 1 cut(s) 85
RsrII CGGWCCG 1 cut(s) 85
SaqAI TTAA 3 cut(s) 162, 618, 669
SatI GCNGC 1 cut(s) 234
Sau3AI GATC 4 cut(s) 70, 207, 226, 384
Sau96I GGNCC 2 cut(s) 85, 556
SchI GAGTC 1 cut(s) 63
SfaNI GCATC 5 cut(s) 126, 315, 337, 424, 616
SinI GGWCC 2 cut(s) 85, 556
SmlI CTYRAG 3 cut(s) 587, 617, 632
SmoI CTYRAG 3 cut(s) 587, 617, 632
Sse9I AATT 5 cut(s) 170, 378, 515, 543, 665
SsiI CCGC 1 cut(s) 16
SspMI CTAG 2 cut(s) 351, 581
StyI CCWWGG 2 cut(s) 99, 252
TasI AATT 5 cut(s) 170, 378, 515, 543, 665
TatI WGTACW 1 cut(s) 246
TfiI GAWTC 1 cut(s) 296
Tru1I TTAA 3 cut(s) 162, 618, 669
Tru9I TTAA 3 cut(s) 162, 618, 669
TscAI CASTG 1 cut(s) 406
TseI GCWGC 1 cut(s) 233
TspDTI ATGAA 3 cut(s) 275, 585, 706
TspGWI ACGGA 1 cut(s) 77
TspRI CASTG 1 cut(s) 406
Vha464I CTTAAG 1 cut(s) 617
VpaK11BI GGWCC 2 cut(s) 85, 556
XapI RAATTY 4 cut(s) 170, 515, 543, 665
XcmI CCANNNNNNNNNTGG 1 cut(s) 604
XmiI GTMKAC 1 cut(s) 190
XmnI GAANNNNTTC 1 cut(s) 149
XspI CTAG 2 cut(s) 351, 581
Zsp2I ATGCAT 1 cut(s) 439
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.