Rh2AG267100

ATP synthase 24 kDa subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0010249
Physical Location & Seq
Forward (+)
31162127 .. 31165863
3737 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG267100.1

Sequence Viewer

Length: 723 bp
ATGGCCTTCTCTTCCCGTCTCGTATCCAAGTCCAGACAGCTGTGTGGCAATGGAGTCGTTCTTCAACAGGAGCGTCTCATTCCGGTTCGTCACTTTGCTAAGGAAGCAAATCGCCCAGTGCTCAAGGGTGATGAGATGCTGAAGAACATCTTCCTGGATGTTAAAAAGAAGTTCGAGACGGCCCTTGGAATACTGCGCAAGGAGAAAATCACCATAGACCCCAAGGATCCAGCTGCAGTGTCTCAATATGCCAACGTCATGAAAACTATTAGAGAGAAGGCGGATTTGTTCTCGGAATCCCAACGTATCCAACACACCATCAAAACCCGTACACAAGATATCCCAGATGCCAGAACTTATCTGTTGACATTGAAGGAAATTAGGATCAGGAGAGGTCTCACTGATGAACTCGGTGCAGAGGCTATGATGATGGATGCATTGGAGAAAGTTGAAAAGGAACTGAAGAAACCTCTTTTGAGGAATGATAAGAAAGGAATGGCTCTTCTCTTGGCAGAGTTTGACAAAGTAAACCAGAAGCTTGGAATTCGGAAGGAAGATTTACCAAAGTATGAAGAACAACTAGAACTCAAGAAAGCTAAGGCACAACTGGAGGAACTTGAGAAGGAGGCTCTTGAGGCAATGGAAACTCAGGCAAAGCGGGAGGAATTCAAAGATGAGGCAATGGTTGATGTAAAGTCTTTGGACATCCGAAACTTCATCTAA

Protein Analysis

240

Amino Acids

27.73

Weight (kDa)

9.17

Isoelectric Point (pI)

29.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mt_ATP_synt PF15704 33 - 219 2e-80 Mitochondrial ATP synthase subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0010249)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G21870 AT2G21870
fragaria_vesca FvH4_1g22890
malus_domestica MD01G1021000.v1.1 MD15G1331100.v1.1
prunus_persica Prupe.6G169300_v2.0.a1
pyrus_communis pycom01g05060 pycom15g29410
rosa_chinensis RchiOBHm_Chr2g0117231
rosa_laevigata RLG00000018337
rosa_multiflora Rmu_co8431947.1_g000001
rosa_roxburghii Rroxscaffold_2G00126620
rosa_rugosa Rorug02G0211500
rosa_samantha Rh2AG267100 Rh2AG268800 Rh2BG279400 Rh2DG274000 Rh2DG275900 Rh2DG293400
rosa_wichuraiana Rw2G021080 Rw2G021130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 197
AciI CCGC 2 cut(s) 281, 658
AclWI GGATC 3 cut(s) 221, 234, 392
AcsI RAATTY 2 cut(s) 543, 665
AcuI CTGAAG 2 cut(s) 161, 482
AfaI GTAC 1 cut(s) 331
AgsI TTSAA 4 cut(s) 65, 373, 452, 670
AjnI CCWGG 1 cut(s) 153
AluBI AGCT 4 cut(s) 40, 233, 538, 596
AluI AGCT 4 cut(s) 40, 233, 538, 596
Alw21I GWGCWC 1 cut(s) 123
Alw26I GTCTC 5 cut(s) 23, 80, 170, 246, 401
AlwI GGATC 3 cut(s) 221, 234, 392
AoxI GGCC 2 cut(s) 3, 180
ApeKI GCWGC 1 cut(s) 233
ApoI RAATTY 2 cut(s) 543, 665
Asp700I GAANNNNTTC 1 cut(s) 149
AspLEI GCGC 1 cut(s) 198
AspS9I GGNCC 1 cut(s) 181
AsuHPI GGTGA 2 cut(s) 140, 202
BamHI GGATCC 1 cut(s) 226
Bbv12I GWGCWC 1 cut(s) 123
BbvI GCAGC 1 cut(s) 220
BccI CCATC 2 cut(s) 326, 424
BceAI ACGGC 1 cut(s) 195
BcgI CGANNNNNNTGC 2 cut(s) 37, 71
BciT130I CCWGG 1 cut(s) 155
BciVI GTATCC 2 cut(s) 34, 317
BcoDI GTCTC 5 cut(s) 23, 80, 170, 246, 401
BfaI CTAG 1 cut(s) 581
BfmI CTRYAG 1 cut(s) 234
BfuI GTATCC 2 cut(s) 34, 317
BisI GCNGC 1 cut(s) 234
BlsI GCNGC 1 cut(s) 235
Bme1390I CCNGG 1 cut(s) 155
BmgT120I GGNCC 1 cut(s) 181
BmiI GGNNCC 1 cut(s) 228
BmrFI CCNGG 1 cut(s) 155
BmrI ACTGGG 1 cut(s) 110
BmsI GCATC 3 cut(s) 126, 337, 424
BmuI ACTGGG 1 cut(s) 110
BpmI CTGGAG 1 cut(s) 629
Bpu10I CCTNAGC 2 cut(s) 99, 597
BpuEI CTTGAG 4 cut(s) 107, 572, 638, 653
BsaI GGTCTC 1 cut(s) 401
BsaJI CCNNGG 2 cut(s) 184, 222
BsaWI WCCGGW 1 cut(s) 82
Bse1I ACTGG 2 cut(s) 116, 612
Bse3DI GCAATG 3 cut(s) 55, 645, 687
BseBI CCWGG 1 cut(s) 155
BseDI CCNNGG 2 cut(s) 184, 222
BseGI GGATG 3 cut(s) 163, 439, 705
BseMI GCAATG 3 cut(s) 55, 645, 687
BseMII CTCAG 1 cut(s) 662
BseNI ACTGG 2 cut(s) 116, 612
BseXI GCAGC 1 cut(s) 220
BsgI GTGCAG 1 cut(s) 435
BshFI GGCC 2 cut(s) 5, 182
BsiHKAI GWGCWC 1 cut(s) 123
BsiSI CCGG 1 cut(s) 83
BsmAI GTCTC 5 cut(s) 23, 80, 170, 246, 401
BsmBI CGTCTC 3 cut(s) 23, 80, 170
BsnI GGCC 2 cut(s) 5, 182
Bso31I GGTCTC 1 cut(s) 401
Bsp1286I GDGCHC 1 cut(s) 123
Bsp143I GATC 2 cut(s) 226, 384
BspACI CCGC 2 cut(s) 281, 658
BspANI GGCC 2 cut(s) 5, 182
BspCNI CTCAG 1 cut(s) 661
BspHI TCATGA 1 cut(s) 258
BspLI GGNNCC 1 cut(s) 228
BspMAI CTGCAG 1 cut(s) 238
BspPI GGATC 3 cut(s) 221, 234, 392
BspQI GCTCTTC 1 cut(s) 507
BspTNI GGTCTC 1 cut(s) 401
BsrDI GCAATG 3 cut(s) 55, 645, 687
BsrI ACTGG 2 cut(s) 116, 612
BssECI CCNNGG 2 cut(s) 184, 222
BssMI GATC 2 cut(s) 226, 384
BssT1I CCWWGG 2 cut(s) 184, 222
Bst2UI CCWGG 1 cut(s) 155
Bst6I CTCTTC 2 cut(s) 16, 507
BstDEI CTNAG 3 cut(s) 99, 597, 648
BstF5I GGATG 3 cut(s) 163, 439, 705
BstHHI GCGC 1 cut(s) 198
BstKTI GATC 2 cut(s) 229, 387
BstMAI GTCTC 5 cut(s) 23, 80, 170, 246, 401
BstMBI GATC 2 cut(s) 226, 384
BstMWI GCNNNNNNNGC 2 cut(s) 104, 635
BstNI CCWGG 1 cut(s) 155
BstSCI CCNGG 1 cut(s) 153
BstSFI CTRYAG 1 cut(s) 234
BstV1I GCAGC 1 cut(s) 220
BstX2I RGATCY 1 cut(s) 226
BstXI CCANNNNNNTGG 1 cut(s) 539
BstYI RGATCY 1 cut(s) 226
BsuI GTATCC 2 cut(s) 34, 317
BsuRI GGCC 2 cut(s) 5, 182
BtsCI GGATG 3 cut(s) 163, 439, 705
BtsI GCAGTG 1 cut(s) 243
BtsIMutI CAGTG 3 cut(s) 123, 243, 399
CciI TCATGA 1 cut(s) 258
CfoI GCGC 1 cut(s) 198
Cfr13I GGNCC 1 cut(s) 181
CseI GACGC 1 cut(s) 62
Csp6I GTAC 1 cut(s) 330
CviAII CATG 1 cut(s) 259
CviJI RGCY 9 cut(s) 5, 40, 182, 233, 422, 500, 538, 596, 629
CviKI_1 RGCY 9 cut(s) 5, 40, 182, 233, 422, 500, 538, 596, 629
CviQI GTAC 1 cut(s) 330
DdeI CTNAG 3 cut(s) 99, 597, 648
DpnI GATC 2 cut(s) 228, 386
DpnII GATC 2 cut(s) 226, 384
Eam1104I CTCTTC 2 cut(s) 16, 507
EarI CTCTTC 2 cut(s) 16, 507
EciI GGCGGA 1 cut(s) 296
Eco130I CCWWGG 2 cut(s) 184, 222
Eco31I GGTCTC 1 cut(s) 401
Eco32I GATATC 1 cut(s) 340
Eco57I CTGAAG 2 cut(s) 161, 482
EcoRI GAATTC 2 cut(s) 543, 665
EcoRII CCWGG 1 cut(s) 153
EcoRV GATATC 1 cut(s) 340
EcoT14I CCWWGG 2 cut(s) 184, 222
EcoT22I ATGCAT 1 cut(s) 439
ErhI CCWWGG 2 cut(s) 184, 222
Esp3I CGTCTC 3 cut(s) 23, 80, 170
FaeI CATG 1 cut(s) 262
FaiI YATR 5 cut(s) 215, 249, 260, 425, 570
FalI AAGNNNNNCTT 2 cut(s) 134, 166
FatI CATG 1 cut(s) 258
FauI CCCGC 1 cut(s) 651
Fnu4HI GCNGC 1 cut(s) 234
FokI GGATG 3 cut(s) 170, 446, 692
Fsp4HI GCNGC 1 cut(s) 234
FspBI CTAG 1 cut(s) 581
FspI TGCGCA 1 cut(s) 197
GlaI GCGC 1 cut(s) 197
GluI GCNGC 1 cut(s) 234
GsuI CTGGAG 1 cut(s) 629
HaeIII GGCC 2 cut(s) 5, 182
HapII CCGG 1 cut(s) 83
HgaI GACGC 1 cut(s) 62
HhaI GCGC 1 cut(s) 198
Hin1II CATG 1 cut(s) 262
Hin6I GCGC 1 cut(s) 196
HinP1I GCGC 1 cut(s) 196
HincII GTYRAC 1 cut(s) 366
HindII GTYRAC 1 cut(s) 366
HindIII AAGCTT 1 cut(s) 536
HinfI GANTC 2 cut(s) 54, 296
HpaII CCGG 1 cut(s) 83
HphI GGTGA 2 cut(s) 140, 202
Hpy166II GTNNAC 3 cut(s) 332, 366, 529
Hpy188I TCNGA 3 cut(s) 295, 549, 710
Hpy188III TCNNGA 6 cut(s) 33, 175, 259, 388, 589, 632
Hpy8I GTNNAC 3 cut(s) 332, 366, 529
HpyAV CCTTC 5 cut(s) 16, 271, 367, 544, 616
HpyCH4IV ACGT 2 cut(s) 255, 304
HpyCH4V TGCA 3 cut(s) 236, 416, 437
HpyF10VI GCNNNNNNNGC 2 cut(s) 104, 635
HpyF3I CTNAG 3 cut(s) 99, 597, 648
HpySE526I ACGT 2 cut(s) 255, 304
Hsp92II CATG 1 cut(s) 262
HspAI GCGC 1 cut(s) 196
Kzo9I GATC 2 cut(s) 226, 384
LguI GCTCTTC 1 cut(s) 507
LmnI GCTCC 1 cut(s) 70
Lsp1109I GCAGC 1 cut(s) 220
LweI GCATC 3 cut(s) 126, 337, 424
MaeI CTAG 1 cut(s) 581
MaeII ACGT 2 cut(s) 255, 304
MaeIII GTNAC 1 cut(s) 89
MalI GATC 2 cut(s) 228, 386
MboI GATC 2 cut(s) 226, 384
MboII GAAGA 8 cut(s) 3, 53, 142, 154, 475, 494, 566, 584
MflI RGATCY 1 cut(s) 226
MhlI GDGCHC 1 cut(s) 123
MluCI AATT 3 cut(s) 378, 543, 665
MlyI GAGTC 1 cut(s) 63
MmeI TCCRAC 1 cut(s) 334
MnlI CCTC 9 cut(s) 386, 412, 471, 480, 604, 619, 628, 655, 670
Mph1103I ATGCAT 1 cut(s) 439
MroXI GAANNNNTTC 1 cut(s) 149
MseI TTAA 1 cut(s) 162
MspA1I CMGCKG 2 cut(s) 40, 233
MspI CCGG 1 cut(s) 83
MspR9I CCNGG 1 cut(s) 155
MvaI CCWGG 1 cut(s) 155
MwoI GCNNNNNNNGC 2 cut(s) 104, 635
NdeII GATC 2 cut(s) 226, 384
NlaIII CATG 1 cut(s) 262
NlaIV GGNNCC 1 cut(s) 228
NmuCI GTSAC 1 cut(s) 89
NsbI TGCGCA 1 cut(s) 197
NsiI ATGCAT 1 cut(s) 439
PagI TCATGA 1 cut(s) 258
PciSI GCTCTTC 1 cut(s) 507
PdmI GAANNNNTTC 1 cut(s) 149
PfeI GAWTC 1 cut(s) 296
PfoI TCCNGGA 1 cut(s) 153
PkrI GCNGC 1 cut(s) 235
PleI GAGTC 1 cut(s) 62
PpsI GAGTC 1 cut(s) 62
Psp6I CCWGG 1 cut(s) 153
PspGI CCWGG 1 cut(s) 153
PspN4I GGNNCC 1 cut(s) 228
PspPI GGNCC 1 cut(s) 181
PstI CTGCAG 1 cut(s) 238
PsuI RGATCY 1 cut(s) 226
PvuII CAGCTG 2 cut(s) 40, 233
RsaI GTAC 1 cut(s) 331
RsaNI GTAC 1 cut(s) 330
SapI GCTCTTC 1 cut(s) 507
SaqAI TTAA 1 cut(s) 162
SatI GCNGC 1 cut(s) 234
Sau3AI GATC 2 cut(s) 226, 384
Sau96I GGNCC 1 cut(s) 181
SchI GAGTC 1 cut(s) 63
ScrFI CCNGG 1 cut(s) 155
SduI GDGCHC 1 cut(s) 123
SetI ASST 8 cut(s) 42, 235, 258, 307, 397, 472, 540, 598
SfaNI GCATC 3 cut(s) 126, 337, 424
SfcI CTRYAG 1 cut(s) 234
SmlI CTYRAG 4 cut(s) 122, 587, 617, 632
SmoI CTYRAG 4 cut(s) 122, 587, 617, 632
Sse9I AATT 3 cut(s) 378, 543, 665
SsiI CCGC 2 cut(s) 281, 658
SspMI CTAG 1 cut(s) 581
StyD4I CCNGG 1 cut(s) 153
StyI CCWWGG 2 cut(s) 184, 222
TaiI ACGT 2 cut(s) 258, 307
TaqI TCGA 1 cut(s) 174
TasI AATT 3 cut(s) 378, 543, 665
TfiI GAWTC 1 cut(s) 296
Tru1I TTAA 1 cut(s) 162
Tru9I TTAA 1 cut(s) 162
TscAI CASTG 3 cut(s) 123, 243, 406
TseFI GTSAC 1 cut(s) 89
TseI GCWGC 1 cut(s) 233
Tsp45I GTSAC 1 cut(s) 89
TspDTI ATGAA 4 cut(s) 275, 420, 585, 706
TspRI CASTG 3 cut(s) 123, 243, 406
XapI RAATTY 2 cut(s) 543, 665
XmnI GAANNNNTTC 1 cut(s) 149
XspI CTAG 1 cut(s) 581
Zsp2I ATGCAT 1 cut(s) 439
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.