pycom15g29490

The RING-variant domain is a C4HC3 zinc-finger like motif found in a number of cellular and viral proteins. Some of these proteins have been shown both in vivo and in vitro to have ubiquitin E3 ligase activity.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0009975
Physical Location & Seq
Forward (+)
26373341 .. 26379386
6046 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 984 bp
ATGGCCGATCACTCAGACGCTTCGCCTCTCATGCCTCCGAAGCAGATCACCGAGCCCAGCGGCGCTAACGATAGATCGACCTCGAAGCGGGCCCTGGCGAACAAATCCAGTGCCGAATTTGCCTTGAAAGTGATGAAGCGTAGGGACTTCATTGCTCCCTGCAAGTGCAAAGGGACTTCCAAGTATGTCCACCGGGAGTGTTTGGATCATTGGAGAGCTGTAAAGGAAGGGTTTGCATTTTCTCATTGCACAACCTGCAAGGCTCCTTATCATTTGAGAGTTCATGTCATTGCTGATAGAAAATGGCGTACCTTGAAATTCAGATTCTTCGTGACGAGGGACATTATATTCATATTTCTGTCTGTTCAACTTGTGATTGCTTCGTTGGCCTATTTGGTTTATTTAATAGATGCTTTACAGCAATTCTGGCTTCGTCTTGCATGGGGTTTTGACAGTGAGCTTAGCTTCTACTATATATGTGGAGCTCTATTGTTTTTCGCTTTGCTTGGGTTGTCTGGCTGCTTCATTACTTGTTACGACCGTAGGGTGCGCAACGATTTGGCTCAGCCATGTCGTGAATTATGCCTTTGTTGCTGTCAACCTGGTGTGTGCGCAGACTGTCATTTACCTGGAACACTTTGTATGTGGACTGACTGTACTACATGCTGGGGAAGTTGTGCAAGTATGGCAGGTGAATGTGGTTGCTTGGGAGGCGCTGGTGAAGCAGGGCTACCAATATTATTTATAATGGCTCTGATTGTGCTGGGACTTTTCACCGTGATTGGGATATTCTATAGTGTCCTCGTAGCTACAATGGTTGGACAACGGATTTGGCAGCGGCATTATCATATACTTGCAAAAAGGATGCTGACAAAAGAATATGTTGTTGAGGATGTTGATGGTGAGATAACAGGATCTGATTGGTCTCCCCCACCTCTCCCAGCTGAGCATATTCAGCAGCTGAAAACGCTCGGCCTACTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RINGv

Protein Analysis

328

Amino Acids

36.7

Weight (kDa)

8.43

Isoelectric Point (pI)

50.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RINGv PF12906 47 - 86 1.5e-08 RING-variant domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 746
AarI CACCTGC 1 cut(s) 680
Acc16I TGCGCA 2 cut(s) 551, 613
Acc36I ACCTGC 2 cut(s) 263, 680
AciI CCGC 3 cut(s) 60, 88, 838
AclWI GGATC 2 cut(s) 213, 922
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 2 cut(s) 116, 317
AfaI GTAC 2 cut(s) 310, 658
AfiI CCNNNNNNNGG 2 cut(s) 87, 783
AgsI TTSAA 3 cut(s) 127, 316, 368
AjnI CCWGG 3 cut(s) 93, 601, 628
AluBI AGCT 7 cut(s) 218, 460, 465, 485, 809, 944, 961
AluI AGCT 7 cut(s) 218, 460, 465, 485, 809, 944, 961
Alw21I GWGCWC 1 cut(s) 487
Alw26I GTCTC 1 cut(s) 930
AlwI GGATC 2 cut(s) 213, 922
AlwNI CAGNNNCTG 2 cut(s) 917, 961
AoxI GGCC 4 cut(s) 3, 90, 387, 973
ApaI GGGCCC 1 cut(s) 94
ApeKI GCWGC 3 cut(s) 519, 835, 958
ApoI RAATTY 2 cut(s) 116, 317
ArsI GACNNNNNNTTYG 2 cut(s) 813, 845
AspLEI GCGC 4 cut(s) 65, 552, 614, 716
AspS9I GGNCC 2 cut(s) 90, 91
AsuC2I CCSGG 1 cut(s) 194
AsuHPI GGTGA 5 cut(s) 40, 704, 731, 766, 914
BaeGI GKGCMC 1 cut(s) 94
BanII GRGCYC 3 cut(s) 57, 94, 487
BarI GAAGNNNNNNTAC 2 cut(s) 714, 746
Bbv12I GWGCWC 1 cut(s) 487
BbvI GCAGC 3 cut(s) 506, 847, 970
BccI CCATC 1 cut(s) 893
BciT130I CCWGG 3 cut(s) 95, 603, 630
BcnI CCSGG 1 cut(s) 194
BcoDI GTCTC 1 cut(s) 930
BfmI CTRYAG 2 cut(s) 793, 980
BfoI RGCGCY 2 cut(s) 66, 717
BfuAI ACCTGC 2 cut(s) 263, 680
BisI GCNGC 5 cut(s) 61, 520, 836, 839, 959
BlpI GCTNAGC 3 cut(s) 461, 564, 945
BlsI GCNGC 5 cut(s) 62, 521, 837, 840, 960
Bme1390I CCNGG 4 cut(s) 95, 194, 603, 630
BmgT120I GGNCC 2 cut(s) 90, 91
BmiI GGNNCC 2 cut(s) 92, 264
BmrFI CCNGG 4 cut(s) 95, 194, 603, 630
BmsI GCATC 2 cut(s) 400, 855
Bpu1102I GCTNAGC 3 cut(s) 461, 564, 945
BpuMI CCSGG 1 cut(s) 194
BsaI GGTCTC 1 cut(s) 930
BsaJI CCNNGG 1 cut(s) 93
Bsc4I CCNNNNNNNGG 2 cut(s) 87, 783
Bse1I ACTGG 1 cut(s) 108
Bse3DI GCAATG 3 cut(s) 150, 244, 288
BseBI CCWGG 3 cut(s) 95, 603, 630
BseDI CCNNGG 1 cut(s) 93
BseGI GGATG 2 cut(s) 870, 898
BseLI CCNNNNNNNGG 2 cut(s) 87, 783
BseMI GCAATG 3 cut(s) 150, 244, 288
BseMII CTCAG 3 cut(s) 27, 578, 936
BseNI ACTGG 1 cut(s) 108
BseSI GKGCMC 1 cut(s) 94
BseXI GCAGC 3 cut(s) 506, 847, 970
BseYI CCCAGC 4 cut(s) 56, 666, 763, 940
Bsh1285I CGRYCG 1 cut(s) 541
BshFI GGCC 4 cut(s) 5, 92, 389, 975
BsiEI CGRYCG 1 cut(s) 541
BsiHKAI GWGCWC 1 cut(s) 487
BsiSI CCGG 1 cut(s) 193
BslFI GGGAC 4 cut(s) 158, 187, 353, 780
BslI CCNNNNNNNGG 2 cut(s) 87, 783
BsmAI GTCTC 1 cut(s) 930
BsmFI GGGAC 4 cut(s) 158, 187, 353, 780
BsnI GGCC 4 cut(s) 5, 92, 389, 975
Bso31I GGTCTC 1 cut(s) 930
Bsp120I GGGCCC 1 cut(s) 90
Bsp1286I GDGCHC 3 cut(s) 57, 94, 487
Bsp143I GATC 5 cut(s) 7, 45, 74, 205, 914
Bsp1720I GCTNAGC 3 cut(s) 461, 564, 945
BspACI CCGC 3 cut(s) 60, 88, 838
BspANI GGCC 4 cut(s) 5, 92, 389, 975
BspCNI CTCAG 3 cut(s) 26, 577, 937
BspLI GGNNCC 2 cut(s) 92, 264
BspMI ACCTGC 2 cut(s) 263, 680
BspPI GGATC 2 cut(s) 213, 922
BspTNI GGTCTC 1 cut(s) 930
BsrDI GCAATG 3 cut(s) 150, 244, 288
BsrI ACTGG 1 cut(s) 108
BssECI CCNNGG 1 cut(s) 93
BssMI GATC 5 cut(s) 7, 45, 74, 205, 914
Bst2UI CCWGG 3 cut(s) 95, 603, 630
Bst4CI ACNGT 5 cut(s) 455, 542, 620, 656, 778
BstAPI GCANNNNNTGC 1 cut(s) 255
BstC8I GCNNGC 1 cut(s) 90
BstDEI CTNAG 4 cut(s) 13, 461, 564, 945
BstF5I GGATG 2 cut(s) 870, 898
BstH2I RGCGCY 2 cut(s) 66, 717
BstHHI GCGC 4 cut(s) 65, 552, 614, 716
BstKTI GATC 5 cut(s) 10, 48, 77, 208, 917
BstMAI GTCTC 1 cut(s) 930
BstMBI GATC 5 cut(s) 7, 45, 74, 205, 914
BstMCI CGRYCG 1 cut(s) 541
BstNI CCWGG 3 cut(s) 95, 603, 630
BstNSI RCATGY 1 cut(s) 666
BstSCI CCNGG 4 cut(s) 93, 192, 601, 628
BstSFI CTRYAG 2 cut(s) 793, 980
BstSLI GKGCMC 1 cut(s) 94
BstV1I GCAGC 3 cut(s) 506, 847, 970
BstX2I RGATCY 1 cut(s) 914
BstYI RGATCY 1 cut(s) 914
BsuRI GGCC 4 cut(s) 5, 92, 389, 975
BtsCI GGATG 2 cut(s) 870, 898
BtsIMutI CAGTG 2 cut(s) 115, 460
BveI ACCTGC 2 cut(s) 263, 680
Cac8I GCNNGC 1 cut(s) 90
CaiI CAGNNNCTG 2 cut(s) 917, 961
CfoI GCGC 4 cut(s) 65, 552, 614, 716
Cfr13I GGNCC 2 cut(s) 90, 91
CseI GACGC 1 cut(s) 26
CsiI ACCWGGT 1 cut(s) 601
Csp6I GTAC 2 cut(s) 309, 657
CviAII CATG 5 cut(s) 31, 284, 441, 570, 663
CviQI GTAC 2 cut(s) 309, 657
DdeI CTNAG 4 cut(s) 13, 461, 564, 945
DpnI GATC 5 cut(s) 9, 47, 76, 207, 916
DpnII GATC 5 cut(s) 7, 45, 74, 205, 914
EaeI YGGCCR 1 cut(s) 3
Ecl136II GAGCTC 1 cut(s) 485
Eco24I GRGCYC 3 cut(s) 57, 94, 487
Eco31I GGTCTC 1 cut(s) 930
Eco53kI GAGCTC 1 cut(s) 485
EcoICRI GAGCTC 1 cut(s) 485
EcoO109I RGGNCCY 1 cut(s) 91
EcoRII CCWGG 3 cut(s) 93, 601, 628
EcoT38I GRGCYC 3 cut(s) 57, 94, 487
FaeI CATG 5 cut(s) 34, 287, 444, 573, 666
FaqI GGGAC 4 cut(s) 158, 187, 353, 780
FatI CATG 5 cut(s) 30, 283, 440, 569, 662
FauI CCCGC 1 cut(s) 81
Fnu4HI GCNGC 5 cut(s) 61, 520, 836, 839, 959
FokI GGATG 2 cut(s) 877, 905
FriOI GRGCYC 3 cut(s) 57, 94, 487
Fsp4HI GCNGC 5 cut(s) 61, 520, 836, 839, 959
FspI TGCGCA 2 cut(s) 551, 613
GlaI GCGC 4 cut(s) 64, 551, 613, 715
GluI GCNGC 5 cut(s) 61, 520, 836, 839, 959
GsaI CCCAGC 4 cut(s) 60, 670, 767, 944
HaeII RGCGCY 2 cut(s) 66, 717
HaeIII GGCC 4 cut(s) 5, 92, 389, 975
HapII CCGG 1 cut(s) 193
HgaI GACGC 1 cut(s) 26
HhaI GCGC 4 cut(s) 65, 552, 614, 716
Hin1II CATG 5 cut(s) 34, 287, 444, 573, 666
Hin6I GCGC 4 cut(s) 63, 550, 612, 714
HinP1I GCGC 4 cut(s) 63, 550, 612, 714
HincII GTYRAC 1 cut(s) 599
HindII GTYRAC 1 cut(s) 599
HinfI GANTC 1 cut(s) 324
HpaII CCGG 1 cut(s) 193
HphI GGTGA 5 cut(s) 40, 704, 731, 766, 914
Hpy166II GTNNAC 3 cut(s) 190, 599, 648
Hpy188I TCNGA 5 cut(s) 16, 39, 323, 756, 919
Hpy188III TCNNGA 2 cut(s) 331, 575
Hpy8I GTNNAC 3 cut(s) 190, 599, 648
HpyAV CCTTC 1 cut(s) 221
HpyCH4III ACNGT 5 cut(s) 455, 542, 620, 656, 778
HpyCH4V TGCA 8 cut(s) 162, 168, 236, 249, 258, 440, 680, 857
HpyF3I CTNAG 4 cut(s) 13, 461, 564, 945
Hsp92II CATG 5 cut(s) 34, 287, 444, 573, 666
HspAI GCGC 4 cut(s) 63, 550, 612, 714
Kzo9I GATC 5 cut(s) 7, 45, 74, 205, 914
LmnI GCTCC 3 cut(s) 160, 268, 482
Lsp1109I GCAGC 3 cut(s) 506, 847, 970
LweI GCATC 2 cut(s) 400, 855
MabI ACCWGGT 1 cut(s) 601
MaeIII GTNAC 2 cut(s) 331, 533
MalI GATC 5 cut(s) 9, 47, 76, 207, 916
MboI GATC 5 cut(s) 7, 45, 74, 205, 914
MboII GAAGA 1 cut(s) 319
MflI RGATCY 1 cut(s) 914
MhlI GDGCHC 3 cut(s) 57, 94, 487
MluCI AATT 4 cut(s) 116, 317, 422, 578
MmeI TCCRAC 1 cut(s) 799
MnlI CCTC 8 cut(s) 36, 45, 91, 330, 704, 812, 883, 945
MseI TTAA 1 cut(s) 404
MspA1I CMGCKG 4 cut(s) 60, 838, 944, 961
MspI CCGG 1 cut(s) 193
MspR9I CCNGG 4 cut(s) 95, 194, 603, 630
MvaI CCWGG 3 cut(s) 95, 603, 630
NciI CCSGG 1 cut(s) 194
NdeII GATC 5 cut(s) 7, 45, 74, 205, 914
NlaIII CATG 5 cut(s) 34, 287, 444, 573, 666
NlaIV GGNNCC 2 cut(s) 92, 264
NmeAIII GCCGAG 1 cut(s) 951
NmuCI GTSAC 1 cut(s) 331
NsbI TGCGCA 2 cut(s) 551, 613
NspI RCATGY 1 cut(s) 666
PaqCI CACCTGC 1 cut(s) 680
PfeI GAWTC 1 cut(s) 324
PkrI GCNGC 5 cut(s) 62, 521, 837, 840, 960
PsiI TTATAA 1 cut(s) 746
Psp124BI GAGCTC 1 cut(s) 487
Psp6I CCWGG 3 cut(s) 93, 601, 628
PspFI CCCAGC 4 cut(s) 56, 666, 763, 940
PspGI CCWGG 3 cut(s) 93, 601, 628
PspN4I GGNNCC 2 cut(s) 92, 264
PspOMI GGGCCC 1 cut(s) 90
PspPI GGNCC 2 cut(s) 90, 91
PstNI CAGNNNCTG 2 cut(s) 917, 961
PsuI RGATCY 1 cut(s) 914
PvuII CAGCTG 2 cut(s) 944, 961
RsaI GTAC 2 cut(s) 310, 658
RsaNI GTAC 2 cut(s) 309, 657
SacI GAGCTC 1 cut(s) 487
SaqAI TTAA 1 cut(s) 404
SatI GCNGC 5 cut(s) 61, 520, 836, 839, 959
Sau3AI GATC 5 cut(s) 7, 45, 74, 205, 914
Sau96I GGNCC 2 cut(s) 90, 91
ScrFI CCNGG 4 cut(s) 95, 194, 603, 630
SduI GDGCHC 3 cut(s) 57, 94, 487
SexAI ACCWGGT 1 cut(s) 601
SfaNI GCATC 2 cut(s) 400, 855
SfcI CTRYAG 2 cut(s) 793, 980
Sse9I AATT 4 cut(s) 116, 317, 422, 578
SsiI CCGC 3 cut(s) 60, 88, 838
SspI AATATT 1 cut(s) 738
SstI GAGCTC 1 cut(s) 487
StyD4I CCNGG 4 cut(s) 93, 192, 601, 628
TaaI ACNGT 5 cut(s) 455, 542, 620, 656, 778
TaqI TCGA 2 cut(s) 77, 83
TasI AATT 4 cut(s) 116, 317, 422, 578
TatI WGTACW 1 cut(s) 656
TauI GCSGC 2 cut(s) 63, 841
TfiI GAWTC 1 cut(s) 324
Tru1I TTAA 1 cut(s) 404
Tru9I TTAA 1 cut(s) 404
TscAI CASTG 2 cut(s) 115, 460
TseFI GTSAC 1 cut(s) 331
TseI GCWGC 3 cut(s) 519, 835, 958
Tsp45I GTSAC 1 cut(s) 331
TspDTI ATGAA 5 cut(s) 139, 149, 272, 340, 514
TspGWI ACGGA 1 cut(s) 841
TspRI CASTG 2 cut(s) 115, 460
XapI RAATTY 2 cut(s) 116, 317
XceI RCATGY 1 cut(s) 666
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.