Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr15

Orthogroup ID

OG0003833

Physical Location & Seq

Genomic Coordinates Forward (+)

29148362 .. 29152990

Gene Structure 4629 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2688 bp

ATGGGCTGCGGTGGCTCCAAGGTCGACGACTTGCCGCTAGTCACGCTTTGCCGGGAGCGGAAGGACTTCATCAAAAAGGCGTTGGACCAGCGCTACGCTCTCGCCGCAGCCCACCTCTCCTACTTCCAATCCCTCCAGCACATCGGCGACGCCCTGTGCAAGTTCGTCGACGAAGACCTTATTATCTCGGGCGCCGGCGGGTCGTCTTCGCCGCCGGGATCCCCAGTTCTCACGTTACCCTCCGACGAAGGCAAGCCCAGGAAAAGGAAACCCCATACAAACGACTCGTCGTCTACGTCGATTTCGCACTCTGTTTCCGCTGATTCGCCGAAAGCGGACGACTTTGACGACTCTCACATAAATTTGTCGTCTGGGTCCGAGTCAGAATCCGAGTTAGACTTGTCGTCGGGTCACATTCACATCGAGGACAGCCCGGAGCAAGAGGTACCCTCTTCTTCTTCCTCCTATCGTTACCCTTCGAGTTACCCTTCGAATTACCCTCCGAATTACCCCCCGAATTACCCTTCGAATTACACTCCGAATTCACATATGTATTATATGAGAAGGACAGAGACGCCGATGCAGACGGTGTATTACGAAGAGCCGGAGAGATATCCGACCCAAAATGGTCCTTACCTGGATCCTTATCCGGGTTATTCGGGGTTTCAGCCATATGGCGGTGGTGGGTTTTTCGGGTACCCAATGGGGTCACCGGTGAATAGTGAAAATCCTTACAATAGACGTCCGCCGAGCCCACCATCACCGGCCCGTCCTCCACCTGCACCGCCTTCGCCTCCGACAACCTCCACTTGGGATTTCCTGAATGTGTTTGAAACTTATGATAATACTGGTTACCCGGGTTCCTATCCGAAGGCTAGATACGGGTACGGGTCCACGACGAGCAGTCCGGACTCGAAGGAGGTGAGGGAGAGAGAGGGAATTCCTGATTTGGAGGATGAGACAGAGCAGGAAGTGTTGAAAGAGGTTCACAAGGAGAAGCGGAAGGCGAATGAAGAGGGTAATGTGAATAGGAATAGGAATCGAAATTCGGGTGAAGGGACTTCAAGGGCTGTGCCATTGAAGCAGCCAAGCAGTGAGGGGAGTTCAGGGACAGTGCCATTGCATAGCAGTGAAAGTTCACACTCTGGGCGTGGGAAGGAGATAAAGAGCAGTCCTGATACAATTGGATCCAAAAACTCTGAAGAGGAATCTGTGAAGAAAAAGAGGGTGAGTTTTGAGTTTGAGGTTGAGGTTGAAGCTGCATCGACGCACGATGTTGGATCTTCTAAAGGGAGTAGCTTAACTACATTGTCAGTCCACGGTCCAAGGGATCTTCAGGAGGTTGTGAAGGAAATCAGGGATGAGTTCGAGACTGCGTCTACTTATGGGAAAGAGGTTGCCATGCTGCTTGAAGTGTGCAAGTTGCCTTATCAGCCTAGAGGTGCTGCACTTAAAGTGGTCTTCTCAAGGATCCTTTACCTTGTAGCACCTTCCATGCTGTCTTCACCCCCTTCATCCAGGCCACCAGTAAAATTAAGTTCTAAGACCGTGAAATTGGCAAAAGCTTACCAGGGAGAACCGGGAAAAGATATCAAGAAGCCTGGAAACCTCTCATCGACTTTGGAGAAACTTTATGCATGGGAGAAAAAATTGTACAAGGAAGTTAAGGACGAAGAAAAGCTACGGGTTGACTATGAAAAGAAGTGCAAGAAATTGAAAAATCTAGATAATCATGGAGCTGAGTCTGCTAAGATTGATGCCACACAGGCTTCTGTACGGAAGTTATTAACCAAAATTAATGTTTGTATCAGAGCTGCTGATACCATATCGAGGAGGATACATAAGTTGAGGGATGAAGAATTGCTTCCCCAAGTCACGGAATTGATTCATGGATTGATAAGAATGTGGAAATCCATGCTTAAATGCCACAAGAAACAGTTCCAAGCTATTATGGAGAGCAAAATTCGATCTCTTAAGGTGAGCACTGGCCTTCGAAAAGATTCCGGTTTGAAAGCTACTCTTGAACTTGAAATGGAGCTTCTGAGCTGGTGCGCCTCCTTTAACAATTGGGTCAGCACCCAAAAGTCCTATGTGGAGTCCTTAAATGGGTGGCTTTCAAGGTGCATTAATCAGGAACCTGAAGAAACTACCGATGGAGTTGCCCCTTTCTCCCCAAGCCGGATGGGAGCTCCACCCATTTTTGTAGTTTGTAATGATTGGTGCCAAGCAATGGAAAGAATTTCCGAAAAGGGGGTGGCAGGTGCGATGCAAGATTTTGCTTCAACCTTACACCAGTTATGGGAAAGGCAGGACGAGGAGCAACGTCAGAGGATCAAAGCTGAGTATGTCTCCAAGAATTTGGAGAGCCAACTCAGAAAATTGCGTATGGAGAGAGCAAAAAGAGACCATGACCATGATGCATCAACAGACAAAAGTGCTCTGTCAAAGTCTCCTTCCGATAGTGGAGTTTCACCACTAGATGATCTGAAGGTGGATTTGGATTCAATGAAGAAGAGATTAGCCGAGGAGAGAGCGAGGCATAAGGAGGCCATCAAATTAGTTAATCACGCAGCTTCCAATAGTTTGCAAGGTGGTTTGGTCCCAATTTTTGGAACTTTAAATAGTTTCACTTCAGAGGCTCTGAAAGTTCACGAGCAAGTCAGACTTCAAGATGCCAGAGGCTCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF630 DUF632

Protein Analysis

896

Amino Acids

99.56

Weight (kDa)

7.1

Isoelectric Point (pI)

53.5

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DUF630	PF04783	1 - 59	2.5e-25	Protein of unknown function (DUF630)
DUF632	PF04782	445 - 764	6.1e-107	Protein of unknown function (DUF632)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0003833)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G21740 AT1G77500
fragaria_vesca	FvH4_2g27210 FvH4_2g27210 FvH4_2g27210 FvH4_2g27210 FvH4_2g27210
malus_domestica	MD08G1161000.v1.1 MD15G1346100.v1.1
prunus_persica	Prupe.1G494000_v2.0.a1 Prupe.1G494000_v2.0.a1 Prupe.1G494000_v2.0.a1 Prupe.1G494000_v2.0.a1 Prupe.1G494000_v2.0.a1 Prupe.1G494000_v2.0.a1 Prupe.1G494000_v2.0.a1 Prupe.1G494000_v2.0.a1
pyrus_communis	pycom08g13820 pycom15g31020
rosa_chinensis	RchiOBHm_Chr6g0296121
rosa_laevigata	RLG00000011728
rosa_multiflora	Rmu_sc0003139.1_g000046
rosa_roxburghii	Rroxscaffold_1G00036630 Rroxscaffold_7G00171810 Rroxscaffold_7G00178240
rosa_rugosa	Rorug06G0257900 Rorug06G0258000 Rorug06G0258100 Rorug06G0258200
rosa_samantha	Rh6AG370700 Rh6BG378300 Rh6CG383900 Rh6DG370700
rosa_wichuraiana	Rw6G032310

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	787, 2249
AatII	GACGTC	1 cut(s)	745
Acc36I	ACCTGC	2 cut(s)	787, 2249
Acc65I	GGTACC	2 cut(s)	445, 696
AccB1I	GGYRCC	4 cut(s)	191, 445, 696, 2220
AccB7I	CCANNNNNTGG	2 cut(s)	2230, 2609
AccBSI	CCGCTC	1 cut(s)	58
AccI	GTMKAC	4 cut(s)	24, 168, 293, 1379
AccIII	TCCGGA	1 cut(s)	907
AcsI	RAATTY	7 cut(s)	361, 541, 939, 1045, 1962, 2238, 2356
AcuI	CTGAAG	5 cut(s)	1221, 1319, 2160, 2507, 2616
AcyI	GRCGYC	4 cut(s)	150, 192, 575, 742
AfaI	GTAC	5 cut(s)	447, 698, 887, 1655, 1776
AfeI	AGCGCT	1 cut(s)	92
AflII	CTTAAG	1 cut(s)	1973
AgeI	ACCGGT	1 cut(s)	712
AhdI	GACNNNNNGTC	3 cut(s)	289, 403, 903
AjnI	CCWGG	5 cut(s)	257, 636, 1517, 1569, 1600
AjuI	GAANNNNNNNTTGG	2 cut(s)	2480, 2512
AloI	GAACNNNNNNTCC	2 cut(s)	210, 242
Alw21I	GWGCWC	3 cut(s)	1985, 2191, 2440
Alw26I	GTCTC	6 cut(s)	566, 953, 1364, 2353, 2397, 2454
Ama87I	CYCGRG	2 cut(s)	187, 856
Aor13HI	TCCGGA	1 cut(s)	907
Aor51HI	AGCGCT	1 cut(s)	92
AoxI	GGCC	4 cut(s)	765, 1520, 1987, 2547
ApeKI	GCWGC	8 cut(s)	6, 107, 1084, 1259, 1405, 1445, 1814, 2570
ApoI	RAATTY	7 cut(s)	361, 541, 939, 1045, 1962, 2238, 2356
ArsI	GACNNNNNNTTYG	2 cut(s)	272, 304
AseI	ATTAAT	2 cut(s)	1797, 2127
AsiGI	ACCGGT	1 cut(s)	712
Asp700I	GAANNNNTTC	5 cut(s)	65, 1863, 1884, 1937, 1999
Asp718I	GGTACC	2 cut(s)	445, 696
AspLEI	GCGC	3 cut(s)	93, 194, 2054
AspS9I	GGNCC	7 cut(s)	85, 375, 629, 766, 891, 1322, 2599
AsuC2I	CCSGG	7 cut(s)	53, 216, 434, 651, 857, 858, 1581
AsuHPI	GGTGA	9 cut(s)	702, 727, 753, 934, 1064, 1240, 1497, 1990, 2463
AsuII	TTCGAA	3 cut(s)	491, 527, 1993
AvaI	CYCGRG	2 cut(s)	187, 856
AvaII	GGWCC	6 cut(s)	85, 375, 629, 891, 1322, 2599
BaeI	ACNNNNGTAYC	2 cut(s)	1829, 1862
BamHI	GGATCC	4 cut(s)	218, 640, 1187, 1470
BanI	GGYRCC	4 cut(s)	191, 445, 696, 2220
BanII	GRGCYC	2 cut(s)	755, 2191
BarI	GAAGNNNNNNTAC	4 cut(s)	863, 895, 1665, 1697
BauI	CACGAG	1 cut(s)	2651
BbsI	GAAGAC	4 cut(s)	180, 198, 1453, 1494
Bbv12I	GWGCWC	3 cut(s)	1985, 2191, 2440
BbvI	GCAGC	7 cut(s)	119, 1096, 1246, 1392, 1432, 1801, 2582
BccI	CCATC	4 cut(s)	766, 2147, 2176, 2558
BcgI	CGANNNNNNTGC	6 cut(s)	148, 182, 771, 805, 2141, 2175
BciT130I	CCWGG	5 cut(s)	259, 638, 1519, 1571, 1602
BciVI	GTATCC	1 cut(s)	1830
BcnI	CCSGG	7 cut(s)	53, 216, 434, 651, 857, 858, 1581
BcoDI	GTCTC	6 cut(s)	566, 953, 1364, 2353, 2397, 2454
BfaI	CTAG	5 cut(s)	38, 876, 1437, 1724, 2477
BfoI	RGCGCY	2 cut(s)	94, 195
BfrI	CTTAAG	1 cut(s)	1973
BfuAI	ACCTGC	2 cut(s)	787, 2249
BfuI	GTATCC	1 cut(s)	1830
BglI	GCCNNNNNGGC	1 cut(s)	1766
Bme18I	GGWCC	6 cut(s)	85, 375, 629, 891, 1322, 2599
BmeRI	GACNNNNNGTC	3 cut(s)	289, 403, 903
BmeT110I	CYCGRG	2 cut(s)	187, 856
BmgT120I	GGNCC	7 cut(s)	85, 375, 629, 766, 891, 1322, 2599
BmrI	ACTGGG	1 cut(s)	218
BmsI	GCATC	7 cut(s)	570, 1271, 1747, 2256, 2407, 2429, 2662
BmuI	ACTGGG	1 cut(s)	218
BpiI	GAAGAC	4 cut(s)	180, 198, 1453, 1494
BplI	GAGNNNNNCTC	4 cut(s)	434, 466, 2333, 2365
BpmI	CTGGAG	1 cut(s)	119
Bpu14I	TTCGAA	3 cut(s)	491, 527, 1993
BpuEI	CTTGAG	1 cut(s)	1450
BpuMI	CCSGG	7 cut(s)	53, 216, 434, 651, 857, 858, 1581
BsaBI	GATNNNNATC	1 cut(s)	645
BsaHI	GRCGYC	4 cut(s)	150, 192, 575, 742
BsaI	GGTCTC	1 cut(s)	2397
BsaJI	CCNNGG	7 cut(s)	18, 257, 856, 1318, 1325, 1570, 2523
BsaWI	WCCGGW	3 cut(s)	712, 907, 2003
Bse118I	RCCGGY	3 cut(s)	194, 712, 763
Bse1I	ACTGG	5 cut(s)	224, 853, 1526, 1990, 2293
Bse3DI	GCAATG	2 cut(s)	1118, 2235
Bse8I	GATNNNNATC	1 cut(s)	645
BseAI	TCCGGA	1 cut(s)	907
BseBI	CCWGG	5 cut(s)	259, 638, 1519, 1571, 1602
BseDI	CCNNGG	7 cut(s)	18, 257, 856, 1318, 1325, 1570, 2523
BseGI	GGATG	5 cut(s)	961, 1366, 1514, 1858, 2187
BseJI	GATNNNNATC	1 cut(s)	645
BseMI	GCAATG	2 cut(s)	1118, 2235
BseMII	CTCAG	4 cut(s)	1731, 2033, 2331, 2386
BseNI	ACTGG	5 cut(s)	224, 853, 1526, 1990, 2293
BseRI	GAGGAG	3 cut(s)	1846, 2330, 2540
BseXI	GCAGC	7 cut(s)	119, 1096, 1246, 1392, 1432, 1801, 2582
BsgI	GTGCAG	2 cut(s)	765, 1431
BshFI	GGCC	4 cut(s)	767, 1522, 1989, 2549
BshNI	GGYRCC	4 cut(s)	191, 445, 696, 2220
BshTI	ACCGGT	1 cut(s)	712
BsiHKAI	GWGCWC	3 cut(s)	1985, 2191, 2440
BsiHKCI	CYCGRG	2 cut(s)	187, 856
BslFI	GGGAC	3 cut(s)	1072, 1123, 2585
BsmAI	GTCTC	6 cut(s)	566, 953, 1364, 2353, 2397, 2454
BsmBI	CGTCTC	1 cut(s)	566
BsmFI	GGGAC	3 cut(s)	1072, 1123, 2585
BsnI	GGCC	4 cut(s)	767, 1522, 1989, 2549
Bso31I	GGTCTC	1 cut(s)	2397
BsoBI	CYCGRG	2 cut(s)	187, 856
Bsp119I	TTCGAA	3 cut(s)	491, 527, 1993
Bsp1286I	GDGCHC	4 cut(s)	755, 1985, 2191, 2440
Bsp13I	TCCGGA	1 cut(s)	907
Bsp1407I	TGTACA	1 cut(s)	1653
Bsp143I	GATC	9 cut(s)	218, 640, 1187, 1280, 1330, 1470, 1967, 2331, 2482
BspANI	GGCC	4 cut(s)	767, 1522, 1989, 2549
BspCNI	CTCAG	4 cut(s)	1732, 2034, 2332, 2385
BspEI	TCCGGA	1 cut(s)	907
BspMI	ACCTGC	2 cut(s)	787, 2249
BspQI	GCTCTTC	1 cut(s)	594
BspT104I	TTCGAA	3 cut(s)	491, 527, 1993
BspT107I	GGYRCC	4 cut(s)	191, 445, 696, 2220
BspTI	CTTAAG	1 cut(s)	1973
BspTNI	GGTCTC	1 cut(s)	2397
BsrBI	CCGCTC	1 cut(s)	58
BsrDI	GCAATG	2 cut(s)	1118, 2235
BsrFI	RCCGGY	3 cut(s)	194, 712, 763
BsrGI	TGTACA	1 cut(s)	1653
BsrI	ACTGG	5 cut(s)	224, 853, 1526, 1990, 2293
BssAI	RCCGGY	3 cut(s)	194, 712, 763
BssECI	CCNNGG	7 cut(s)	18, 257, 856, 1318, 1325, 1570, 2523
BssMI	GATC	9 cut(s)	218, 640, 1187, 1280, 1330, 1470, 1967, 2331, 2482
BssNI	GRCGYC	4 cut(s)	150, 192, 575, 742
BssSI	CACGAG	1 cut(s)	2651
BssT1I	CCWWGG	2 cut(s)	18, 1325
Bst2BI	CACGAG	1 cut(s)	2651
Bst2UI	CCWGG	5 cut(s)	259, 638, 1519, 1571, 1602
Bst4CI	ACNGT	5 cut(s)	589, 1114, 1322, 1549, 1938
Bst6I	CTCTTC	5 cut(s)	457, 594, 1008, 1197, 2507
BstACI	GRCGYC	4 cut(s)	150, 192, 575, 742
BstAFI	CTTAAG	1 cut(s)	1973
BstAUI	TGTACA	1 cut(s)	1653
BstBI	TTCGAA	3 cut(s)	491, 527, 1993
BstC8I	GCNNGC	2 cut(s)	196, 254
BstDEI	CTNAG	6 cut(s)	1542, 1740, 1749, 2042, 2340, 2372
BstDSI	CCRYGG	1 cut(s)	1318
BstEII	GGTNACC	2 cut(s)	708, 851
BstF5I	GGATG	5 cut(s)	961, 1366, 1514, 1858, 2187
BstH2I	RGCGCY	2 cut(s)	94, 195
BstHHI	GCGC	3 cut(s)	93, 194, 2054
BstKTI	GATC	9 cut(s)	221, 643, 1190, 1283, 1333, 1473, 1970, 2334, 2485
BstMAI	GTCTC	6 cut(s)	566, 953, 1364, 2353, 2397, 2454
BstMBI	GATC	9 cut(s)	218, 640, 1187, 1280, 1330, 1470, 1967, 2331, 2482
BstMWI	GCNNNNNNNGC	7 cut(s)	12, 43, 104, 1081, 1432, 1745, 1766
BstNI	CCWGG	5 cut(s)	259, 638, 1519, 1571, 1602
BstPI	GGTNACC	2 cut(s)	708, 851
BstV1I	GCAGC	7 cut(s)	119, 1096, 1246, 1392, 1432, 1801, 2582
BstV2I	GAAGAC	4 cut(s)	180, 198, 1453, 1494
BstX2I	RGATCY	6 cut(s)	218, 640, 1187, 1280, 1330, 1470
BstXI	CCANNNNNNTGG	1 cut(s)	2359
BstYI	RGATCY	6 cut(s)	218, 640, 1187, 1280, 1330, 1470
BsuI	GTATCC	1 cut(s)	1830
BsuRI	GGCC	4 cut(s)	767, 1522, 1989, 2549
BtgI	CCRYGG	1 cut(s)	1318
BtgZI	GCGATG	1 cut(s)	2279
BtsCI	GGATG	5 cut(s)	961, 1366, 1514, 1858, 2187
BtsI	GCAGTG	2 cut(s)	1099, 1135
BtsIMutI	CAGTG	4 cut(s)	1099, 1119, 1135, 1983
BveI	ACCTGC	2 cut(s)	787, 2249
Cac8I	GCNNGC	2 cut(s)	196, 254
CfoI	GCGC	3 cut(s)	93, 194, 2054
Cfr10I	RCCGGY	3 cut(s)	194, 712, 763
Cfr13I	GGNCC	7 cut(s)	85, 375, 629, 766, 891, 1322, 2599
Cfr9I	CCCGGG	1 cut(s)	856
CseI	GACGC	4 cut(s)	158, 583, 1276, 1365
Csp6I	GTAC	5 cut(s)	446, 697, 886, 1654, 1775
CspAI	ACCGGT	1 cut(s)	712
CviAII	CATG	8 cut(s)	1402, 1495, 1638, 1733, 1889, 1915, 2408, 2414
CviQI	GTAC	5 cut(s)	446, 697, 886, 1654, 1775
DdeI	CTNAG	6 cut(s)	1542, 1740, 1749, 2042, 2340, 2372
DinI	GGCGCC	1 cut(s)	193
DpnI	GATC	9 cut(s)	220, 642, 1189, 1282, 1332, 1472, 1969, 2333, 2484
DpnII	GATC	9 cut(s)	218, 640, 1187, 1280, 1330, 1470, 1967, 2331, 2482
DraI	TTTAAA	1 cut(s)	2619
DriI	GACNNNNNGTC	3 cut(s)	289, 403, 903
Eam1104I	CTCTTC	5 cut(s)	457, 594, 1008, 1197, 2507
Eam1105I	GACNNNNNGTC	3 cut(s)	289, 403, 903
EarI	CTCTTC	5 cut(s)	457, 594, 1008, 1197, 2507
EciI	GGCGGA	1 cut(s)	735
Ecl136II	GAGCTC	1 cut(s)	2189
Eco130I	CCWWGG	2 cut(s)	18, 1325
Eco24I	GRGCYC	2 cut(s)	755, 2191
Eco31I	GGTCTC	1 cut(s)	2397
Eco32I	GATATC	2 cut(s)	614, 1591
Eco47I	GGWCC	6 cut(s)	85, 375, 629, 891, 1322, 2599
Eco47III	AGCGCT	1 cut(s)	92
Eco53kI	GAGCTC	1 cut(s)	2189
Eco57I	CTGAAG	5 cut(s)	1221, 1319, 2160, 2507, 2616
Eco88I	CYCGRG	2 cut(s)	187, 856
Eco91I	GGTNACC	2 cut(s)	708, 851
EcoICRI	GAGCTC	1 cut(s)	2189
EcoO65I	GGTNACC	2 cut(s)	708, 851
EcoRI	GAATTC	2 cut(s)	541, 939
EcoRII	CCWGG	5 cut(s)	257, 636, 1517, 1569, 1600
EcoRV	GATATC	2 cut(s)	614, 1591
EcoT14I	CCWWGG	2 cut(s)	18, 1325
EcoT22I	ATGCAT	2 cut(s)	1639, 2422
EcoT38I	GRGCYC	2 cut(s)	755, 2191
EgeI	GGCGCC	1 cut(s)	193
EheI	GGCGCC	1 cut(s)	193
ErhI	CCWWGG	2 cut(s)	18, 1325
Esp3I	CGTCTC	1 cut(s)	566
FaeI	CATG	8 cut(s)	1405, 1498, 1641, 1736, 1892, 1918, 2411, 2417
FalI	AAGNNNNNCTT	6 cut(s)	1848, 1880, 2004, 2036, 2649, 2681
FaqI	GGGAC	3 cut(s)	1072, 1123, 2585
FatI	CATG	8 cut(s)	1401, 1494, 1637, 1732, 1888, 1914, 2407, 2413
FauI	CCCGC	1 cut(s)	191
FauNDI	CATATG	2 cut(s)	549, 673
FblI	GTMKAC	4 cut(s)	24, 168, 293, 1379
FokI	GGATG	5 cut(s)	968, 1373, 1501, 1865, 2194
FriOI	GRGCYC	2 cut(s)	755, 2191
FspBI	CTAG	5 cut(s)	38, 876, 1437, 1724, 2477
GlaI	GCGC	3 cut(s)	92, 193, 2053
GsuI	CTGGAG	1 cut(s)	119
HaeII	RGCGCY	2 cut(s)	94, 195
HaeIII	GGCC	4 cut(s)	767, 1522, 1989, 2549
HgaI	GACGC	4 cut(s)	158, 583, 1276, 1365
HhaI	GCGC	3 cut(s)	93, 194, 2054
Hin1I	GRCGYC	4 cut(s)	150, 192, 575, 742
Hin1II	CATG	8 cut(s)	1405, 1498, 1641, 1736, 1892, 1918, 2411, 2417
Hin6I	GCGC	3 cut(s)	91, 192, 2052
HinP1I	GCGC	3 cut(s)	91, 192, 2052
HincII	GTYRAC	3 cut(s)	25, 169, 1690
HindII	GTYRAC	3 cut(s)	25, 169, 1690
HindIII	AAGCTT	1 cut(s)	1563
HphI	GGTGA	9 cut(s)	702, 727, 753, 934, 1064, 1240, 1497, 1990, 2463
HpyCH4III	ACNGT	5 cut(s)	589, 1114, 1322, 1549, 1938
HpyCH4IV	ACGT	4 cut(s)	233, 296, 742, 2323
HpyF10VI	GCNNNNNNNGC	7 cut(s)	12, 43, 104, 1081, 1432, 1745, 1766
HpyF3I	CTNAG	6 cut(s)	1542, 1740, 1749, 2042, 2340, 2372
HpySE526I	ACGT	4 cut(s)	233, 296, 742, 2323
Hsp92I	GRCGYC	4 cut(s)	150, 192, 575, 742
Hsp92II	CATG	8 cut(s)	1405, 1498, 1641, 1736, 1892, 1918, 2411, 2417
HspAI	GCGC	3 cut(s)	91, 192, 2052
KasI	GGCGCC	1 cut(s)	191
Kpn2I	TCCGGA	1 cut(s)	907
KpnI	GGTACC	2 cut(s)	449, 700
KroI	GCCGGC	1 cut(s)	194
KroNI	GCCGGC	1 cut(s)	196
Kzo9I	GATC	9 cut(s)	218, 640, 1187, 1280, 1330, 1470, 1967, 2331, 2482
LguI	GCTCTTC	1 cut(s)	594
LmnI	GCTCC	8 cut(s)	20, 55, 436, 1736, 2035, 2186, 2194, 2317
Lsp1109I	GCAGC	7 cut(s)	119, 1096, 1246, 1392, 1432, 1801, 2582
LweI	GCATC	7 cut(s)	570, 1271, 1747, 2256, 2407, 2429, 2662
MaeI	CTAG	5 cut(s)	38, 876, 1437, 1724, 2477
MaeII	ACGT	4 cut(s)	233, 296, 742, 2323
MaeIII	GTNAC	8 cut(s)	40, 234, 410, 470, 482, 708, 851, 1873
MalI	GATC	9 cut(s)	220, 642, 1189, 1282, 1332, 1472, 1969, 2333, 2484
MbiI	CCGCTC	1 cut(s)	58
MboI	GATC	9 cut(s)	218, 640, 1187, 1280, 1330, 1470, 1967, 2331, 2482
MfeI	CAATTG	2 cut(s)	1182, 2065
MflI	RGATCY	6 cut(s)	218, 640, 1187, 1280, 1330, 1470
MhlI	GDGCHC	4 cut(s)	755, 1985, 2191, 2440
Mly113I	GGCGCC	1 cut(s)	192
MlyI	GAGTC	6 cut(s)	278, 344, 389, 905, 1751, 2105
MmeI	TCCRAC	5 cut(s)	63, 267, 641, 821, 1258
Mph1103I	ATGCAT	2 cut(s)	1639, 2422
MreI	CGCCGGCG	1 cut(s)	194
MroI	TCCGGA	1 cut(s)	907
MroNI	GCCGGC	1 cut(s)	194
MroXI	GAANNNNTTC	5 cut(s)	65, 1863, 1884, 1937, 1999
MslI	CAYNNNNRTG	2 cut(s)	1128, 2412
MspA1I	CMGCKG	1 cut(s)	320
MspCI	CTTAAG	1 cut(s)	1973
MunI	CAATTG	2 cut(s)	1182, 2065
MvaI	CCWGG	5 cut(s)	259, 638, 1519, 1571, 1602
MwoI	GCNNNNNNNGC	7 cut(s)	12, 43, 104, 1081, 1432, 1745, 1766
NaeI	GCCGGC	1 cut(s)	196
NarI	GGCGCC	1 cut(s)	192
NciI	CCSGG	7 cut(s)	53, 216, 434, 651, 857, 858, 1581
NdeI	CATATG	2 cut(s)	549, 673
NdeII	GATC	9 cut(s)	218, 640, 1187, 1280, 1330, 1470, 1967, 2331, 2482
NgoMIV	GCCGGC	1 cut(s)	194
NlaIII	CATG	8 cut(s)	1405, 1498, 1641, 1736, 1892, 1918, 2411, 2417
NmeAIII	GCCGAG	2 cut(s)	774, 2548
NmuCI	GTSAC	4 cut(s)	40, 410, 708, 1873
NsiI	ATGCAT	2 cut(s)	1639, 2422
NspV	TTCGAA	3 cut(s)	491, 527, 1993
PaqCI	CACCTGC	2 cut(s)	787, 2249
PciSI	GCTCTTC	1 cut(s)	594
PcsI	WCGNNNNNNNCGW	3 cut(s)	293, 296, 345
PdiI	GCCGGC	1 cut(s)	196
PdmI	GAANNNNTTC	5 cut(s)	65, 1863, 1884, 1937, 1999
PfeI	GAWTC	7 cut(s)	323, 386, 1039, 1208, 1885, 2000, 2501
PflFI	GACNNNGTC	1 cut(s)	1375
PflMI	CCANNNNNTGG	2 cut(s)	2230, 2609
PinAI	ACCGGT	1 cut(s)	712
PleI	GAGTC	6 cut(s)	278, 344, 388, 905, 1750, 2104
PluTI	GGCGCC	1 cut(s)	195
PpsI	GAGTC	6 cut(s)	278, 344, 388, 905, 1750, 2104
PshBI	ATTAAT	2 cut(s)	1797, 2127
Psp124BI	GAGCTC	1 cut(s)	2191
Psp6I	CCWGG	5 cut(s)	257, 636, 1517, 1569, 1600
PspEI	GGTNACC	2 cut(s)	708, 851
PspGI	CCWGG	5 cut(s)	257, 636, 1517, 1569, 1600
PspPI	GGNCC	7 cut(s)	85, 375, 629, 766, 891, 1322, 2599
PsuI	RGATCY	6 cut(s)	218, 640, 1187, 1280, 1330, 1470
PsyI	GACNNNGTC	1 cut(s)	1375
RsaI	GTAC	5 cut(s)	447, 698, 887, 1655, 1776
RsaNI	GTAC	5 cut(s)	446, 697, 886, 1654, 1775
RseI	CAYNNNNRTG	2 cut(s)	1128, 2412
SacI	GAGCTC	1 cut(s)	2191
SalI	GTCGAC	2 cut(s)	23, 167
SapI	GCTCTTC	1 cut(s)	594
Sau3AI	GATC	9 cut(s)	218, 640, 1187, 1280, 1330, 1470, 1967, 2331, 2482
Sau96I	GGNCC	7 cut(s)	85, 375, 629, 766, 891, 1322, 2599
SchI	GAGTC	6 cut(s)	278, 344, 389, 905, 1751, 2105
SduI	GDGCHC	4 cut(s)	755, 1985, 2191, 2440
SfaNI	GCATC	7 cut(s)	570, 1271, 1747, 2256, 2407, 2429, 2662
SfoI	GGCGCC	1 cut(s)	193
SfuI	TTCGAA	3 cut(s)	491, 527, 1993
SgrAI	CRCCGGYG	2 cut(s)	194, 712
SgrDI	CGTCGACG	1 cut(s)	167
SinI	GGWCC	6 cut(s)	85, 375, 629, 891, 1322, 2599
SmaI	CCCGGG	1 cut(s)	858
SmiMI	CAYNNNNRTG	2 cut(s)	1128, 2412
SmlI	CTYRAG	2 cut(s)	1465, 1973
SmoI	CTYRAG	2 cut(s)	1465, 1973
SspDI	GGCGCC	1 cut(s)	191
SspMI	CTAG	5 cut(s)	38, 876, 1437, 1724, 2477
SstI	GAGCTC	1 cut(s)	2191
StyI	CCWWGG	2 cut(s)	18, 1325
TaaI	ACNGT	5 cut(s)	589, 1114, 1322, 1549, 1938
TaiI	ACGT	4 cut(s)	236, 299, 745, 2326
TatI	WGTACW	1 cut(s)	1653
TauI	GCSGC	3 cut(s)	37, 107, 214
TfiI	GAWTC	7 cut(s)	323, 386, 1039, 1208, 1885, 2000, 2501
TscAI	CASTG	4 cut(s)	1099, 1119, 1135, 1990
TseFI	GTSAC	4 cut(s)	40, 410, 708, 1873
TseI	GCWGC	8 cut(s)	6, 107, 1084, 1259, 1405, 1445, 1814, 2570
Tsp45I	GTSAC	4 cut(s)	40, 410, 708, 1873
TspDTI	ATGAA	7 cut(s)	58, 1026, 1503, 1710, 1869, 1877, 2522
TspGWI	ACGGA	2 cut(s)	1792, 1892
TspMI	CCCGGG	1 cut(s)	856
TspRI	CASTG	4 cut(s)	1099, 1119, 1135, 1990
Tth111I	GACNNNGTC	1 cut(s)	1375
Van91I	CCANNNNNTGG	2 cut(s)	2230, 2609
Vha464I	CTTAAG	1 cut(s)	1973
VpaK11BI	GGWCC	6 cut(s)	85, 375, 629, 891, 1322, 2599
VspI	ATTAAT	2 cut(s)	1797, 2127
XapI	RAATTY	7 cut(s)	361, 541, 939, 1045, 1962, 2238, 2356
XbaI	TCTAGA	1 cut(s)	1723
XmaI	CCCGGG	1 cut(s)	856
XmiI	GTMKAC	4 cut(s)	24, 168, 293, 1379
XmnI	GAANNNNTTC	5 cut(s)	65, 1863, 1884, 1937, 1999
XspI	CTAG	5 cut(s)	38, 876, 1437, 1724, 2477
ZraI	GACGTC	1 cut(s)	743
Zsp2I	ATGCAT	2 cut(s)	1639, 2422

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.