pycom15g36690

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0012625
Physical Location & Seq
Forward (+)
36661312 .. 36661854
543 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 543 bp
ATGGGGGCTATATTGAGCTTGAATCATTCCAGGACTAAGGTCAGTGAACCTTCTCAAGATTTGCCGAGTGCATCATGCAAGAGGATGAAGTTGTCTCCAAGCTTTTTTGACGAAAACCCAAGATTGATTCCCTGCATTCCTGACGAGATATCTGTTCAGATTCTTGCCAGAATTCCTAGGATCCACTATTTGAAGCTCAAGTCTGTTTCACGGACCTGGAAAGCCACCATTACAAGTCCTGAACTTTTTTCTTTGAGAAAAGAACTAGGAACAACGGAGGAATGGCTCTACATGTTAACAAAGGTCCAAAGTGATAAGCTTGTATGGTGTGCTCTGGATCCTCTGGCCGGAAAATGGCAAAGGTTGCCATCTATGCCTAATGTTACTCCGGAAGAAGAATCTAGGAAGGGTTTAACTGGCCAGAGAACGTGGAACATGGCAGGCTCCAGTATCAGAATTGCGGATGTCATCATGGGTTGGCTTGGGAGGAAGGATTCACTGGGATCGAATGCCTTTTTGTGGTTGCTCTATTGGGGCTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like Kelch_1

Protein Analysis

181

Amino Acids

20.51

Weight (kDa)

9.48

Isoelectric Point (pI)

51.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 43 - 83 3.7e-08 F-box domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 388
AciI CCGC 1 cut(s) 461
AclWI GGATC 5 cut(s) 175, 188, 332, 345, 511
AcoI YGGCCR 2 cut(s) 345, 418
AcsI RAATTY 1 cut(s) 171
AfiI CCNNNNNNNGG 3 cut(s) 347, 354, 519
AflIII ACRYGT 1 cut(s) 291
AgsI TTSAA 2 cut(s) 22, 193
AjnI CCWGG 2 cut(s) 29, 215
AluBI AGCT 4 cut(s) 18, 102, 196, 319
AluI AGCT 4 cut(s) 18, 102, 196, 319
Alw21I GWGCWC 1 cut(s) 334
Alw26I GTCTC 1 cut(s) 99
AlwI GGATC 5 cut(s) 175, 188, 332, 345, 511
Aor13HI TCCGGA 1 cut(s) 388
AoxI GGCC 2 cut(s) 345, 418
ApoI RAATTY 1 cut(s) 171
AspA2I CCTAGG 1 cut(s) 176
AspS9I GGNCC 2 cut(s) 213, 304
AvaII GGWCC 2 cut(s) 213, 304
AvrII CCTAGG 1 cut(s) 176
BalI TGGCCA 1 cut(s) 420
BamHI GGATCC 2 cut(s) 180, 337
Bbv12I GWGCWC 1 cut(s) 334
BccI CCATC 1 cut(s) 376
BciT130I CCWGG 2 cut(s) 31, 217
BcoDI GTCTC 1 cut(s) 99
BfaI CTAG 3 cut(s) 177, 266, 402
BlnI CCTAGG 1 cut(s) 176
Bme1390I CCNGG 2 cut(s) 31, 217
Bme18I GGWCC 2 cut(s) 213, 304
BmgT120I GGNCC 2 cut(s) 213, 304
BmiI GGNNCC 3 cut(s) 182, 339, 445
BmrFI CCNGG 2 cut(s) 31, 217
BmrI ACTGGG 1 cut(s) 509
BmsI GCATC 1 cut(s) 80
BmuI ACTGGG 1 cut(s) 509
BoxI GACNNNNGTC 1 cut(s) 38
BpmI CTGGAG 1 cut(s) 430
BpuEI CTTGAG 2 cut(s) 39, 182
BsaJI CCNNGG 1 cut(s) 176
BsaWI WCCGGW 1 cut(s) 388
Bsc4I CCNNNNNNNGG 3 cut(s) 347, 354, 519
Bse1I ACTGG 3 cut(s) 421, 447, 504
BseAI TCCGGA 1 cut(s) 388
BseBI CCWGG 2 cut(s) 31, 217
BseDI CCNNGG 1 cut(s) 176
BseGI GGATG 2 cut(s) 90, 469
BseLI CCNNNNNNNGG 3 cut(s) 347, 354, 519
BseNI ACTGG 3 cut(s) 421, 447, 504
BshFI GGCC 2 cut(s) 347, 420
BsiHKAI GWGCWC 1 cut(s) 334
BsiSI CCGG 2 cut(s) 348, 389
BslI CCNNNNNNNGG 3 cut(s) 347, 354, 519
BsmAI GTCTC 1 cut(s) 99
BsmI GAATGC 2 cut(s) 135, 514
BsnI GGCC 2 cut(s) 347, 420
Bsp1286I GDGCHC 1 cut(s) 334
Bsp13I TCCGGA 1 cut(s) 388
Bsp143I GATC 3 cut(s) 180, 337, 503
BspACI CCGC 1 cut(s) 461
BspANI GGCC 2 cut(s) 347, 420
BspEI TCCGGA 1 cut(s) 388
BspLI GGNNCC 3 cut(s) 182, 339, 445
BspPI GGATC 5 cut(s) 175, 188, 332, 345, 511
BsrI ACTGG 3 cut(s) 421, 447, 504
BssECI CCNNGG 1 cut(s) 176
BssMI GATC 3 cut(s) 180, 337, 503
BssT1I CCWWGG 1 cut(s) 176
Bst2UI CCWGG 2 cut(s) 31, 217
BstAPI GCANNNNNTGC 1 cut(s) 364
BstC8I GCNNGC 1 cut(s) 442
BstDEI CTNAG 1 cut(s) 36
BstF5I GGATG 2 cut(s) 90, 469
BstKTI GATC 3 cut(s) 183, 340, 506
BstMAI GTCTC 1 cut(s) 99
BstMBI GATC 3 cut(s) 180, 337, 503
BstMWI GCNNNNNNNGC 2 cut(s) 364, 373
BstNI CCWGG 2 cut(s) 31, 217
BstNSI RCATGY 1 cut(s) 295
BstPAI GACNNNNGTC 1 cut(s) 38
BstSCI CCNGG 2 cut(s) 29, 215
BstX2I RGATCY 2 cut(s) 180, 337
BstYI RGATCY 2 cut(s) 180, 337
BsuRI GGCC 2 cut(s) 347, 420
BtsCI GGATG 2 cut(s) 90, 469
BtsIMutI CAGTG 2 cut(s) 49, 497
Cac8I GCNNGC 1 cut(s) 442
Cfr13I GGNCC 2 cut(s) 213, 304
CviAII CATG 4 cut(s) 75, 292, 436, 472
DdeI CTNAG 1 cut(s) 36
DpnI GATC 3 cut(s) 182, 339, 505
DpnII GATC 3 cut(s) 180, 337, 503
EaeI YGGCCR 2 cut(s) 345, 418
Eco130I CCWWGG 1 cut(s) 176
Eco32I GATATC 1 cut(s) 150
Eco47I GGWCC 2 cut(s) 213, 304
EcoRI GAATTC 1 cut(s) 171
EcoRII CCWGG 2 cut(s) 29, 215
EcoRV GATATC 1 cut(s) 150
EcoT14I CCWWGG 1 cut(s) 176
ErhI CCWWGG 1 cut(s) 176
FaeI CATG 4 cut(s) 78, 295, 439, 475
FaiI YATR 7 cut(s) 11, 76, 293, 325, 374, 437, 473
FatI CATG 4 cut(s) 74, 291, 435, 471
FokI GGATG 2 cut(s) 97, 476
FspBI CTAG 3 cut(s) 177, 266, 402
GsuI CTGGAG 1 cut(s) 430
HaeIII GGCC 2 cut(s) 347, 420
HapII CCGG 2 cut(s) 348, 389
Hin1II CATG 4 cut(s) 78, 295, 439, 475
HincII GTYRAC 1 cut(s) 297
HindII GTYRAC 1 cut(s) 297
HindIII AAGCTT 2 cut(s) 100, 317
HinfI GANTC 5 cut(s) 22, 127, 160, 398, 494
HpaI GTTAAC 1 cut(s) 297
HpaII CCGG 2 cut(s) 348, 389
Hpy166II GTNNAC 2 cut(s) 47, 297
Hpy188I TCNGA 2 cut(s) 159, 455
Hpy188III TCNNGA 5 cut(s) 56, 140, 239, 335, 389
Hpy8I GTNNAC 2 cut(s) 47, 297
HpyAV CCTTC 3 cut(s) 60, 400, 484
HpyCH4IV ACGT 1 cut(s) 428
HpyCH4V TGCA 3 cut(s) 71, 78, 135
HpyF10VI GCNNNNNNNGC 2 cut(s) 364, 373
HpyF3I CTNAG 1 cut(s) 36
HpySE526I ACGT 1 cut(s) 428
Hsp92II CATG 4 cut(s) 78, 295, 439, 475
Kpn2I TCCGGA 1 cut(s) 388
KspAI GTTAAC 1 cut(s) 297
Kzo9I GATC 3 cut(s) 180, 337, 503
LmnI GCTCC 1 cut(s) 449
LweI GCATC 1 cut(s) 80
MaeI CTAG 3 cut(s) 177, 266, 402
MaeII ACGT 1 cut(s) 428
MaeIII GTNAC 1 cut(s) 382
MalI GATC 3 cut(s) 182, 339, 505
MboI GATC 3 cut(s) 180, 337, 503
MboII GAAGA 2 cut(s) 404, 407
MflI RGATCY 2 cut(s) 180, 337
MhlI GDGCHC 1 cut(s) 334
MlsI TGGCCA 1 cut(s) 420
MluCI AATT 2 cut(s) 171, 456
MluNI TGGCCA 1 cut(s) 420
MnlI CCTC 4 cut(s) 75, 271, 351, 480
Mox20I TGGCCA 1 cut(s) 420
MroI TCCGGA 1 cut(s) 388
MscI TGGCCA 1 cut(s) 420
MseI TTAA 2 cut(s) 296, 413
Msp20I TGGCCA 1 cut(s) 420
MspI CCGG 2 cut(s) 348, 389
MspR9I CCNGG 2 cut(s) 31, 217
Mva1269I GAATGC 2 cut(s) 135, 514
MvaI CCWGG 2 cut(s) 31, 217
MwoI GCNNNNNNNGC 2 cut(s) 364, 373
NdeII GATC 3 cut(s) 180, 337, 503
NlaIII CATG 4 cut(s) 78, 295, 439, 475
NlaIV GGNNCC 3 cut(s) 182, 339, 445
NmeAIII GCCGAG 1 cut(s) 90
NspI RCATGY 1 cut(s) 295
PciI ACATGT 1 cut(s) 291
PctI GAATGC 2 cut(s) 135, 514
PfeI GAWTC 5 cut(s) 22, 127, 160, 398, 494
PfoI TCCNGGA 1 cut(s) 29
PscI ACATGT 1 cut(s) 291
PshAI GACNNNNGTC 1 cut(s) 38
Psp6I CCWGG 2 cut(s) 29, 215
PspGI CCWGG 2 cut(s) 29, 215
PspN4I GGNNCC 3 cut(s) 182, 339, 445
PspPI GGNCC 2 cut(s) 213, 304
PsuI RGATCY 2 cut(s) 180, 337
SaqAI TTAA 2 cut(s) 296, 413
Sau3AI GATC 3 cut(s) 180, 337, 503
Sau96I GGNCC 2 cut(s) 213, 304
ScrFI CCNGG 2 cut(s) 31, 217
SduI GDGCHC 1 cut(s) 334
SfaNI GCATC 1 cut(s) 80
SinI GGWCC 2 cut(s) 213, 304
SmlI CTYRAG 2 cut(s) 54, 197
SmoI CTYRAG 2 cut(s) 54, 197
Sse9I AATT 2 cut(s) 171, 456
SsiI CCGC 1 cut(s) 461
SspMI CTAG 3 cut(s) 177, 266, 402
StyD4I CCNGG 2 cut(s) 29, 215
StyI CCWWGG 1 cut(s) 176
TaiI ACGT 1 cut(s) 431
TaqI TCGA 1 cut(s) 506
TasI AATT 2 cut(s) 171, 456
TfiI GAWTC 5 cut(s) 22, 127, 160, 398, 494
Tru1I TTAA 2 cut(s) 296, 413
Tru9I TTAA 2 cut(s) 296, 413
TscAI CASTG 2 cut(s) 49, 504
TspDTI ATGAA 1 cut(s) 101
TspGWI ACGGA 2 cut(s) 226, 290
TspRI CASTG 2 cut(s) 49, 504
VpaK11BI GGWCC 2 cut(s) 213, 304
XapI RAATTY 1 cut(s) 171
XceI RCATGY 1 cut(s) 295
XmaJI CCTAGG 1 cut(s) 176
XspI CTAG 3 cut(s) 177, 266, 402
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.