pycom15g38420

YT521-B-like domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0004199
Physical Location & Seq
Reverse (-)
38202650 .. 38204644
1995 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g38420.1

Sequence Viewer

Length: 1344 bp
ATGGTGTATTACCTTCCCAGCTATAACCCATATGCCCCAGGAACTCTGATGGGAGCGGACGGGCAAGGTGTTGGCCAACAACAGTATTATCCTTCCTCAGGATATATGCAACCACCTGTTTCATATGGATCAGAAGCTGCGCCTTGTTATTCCTGGGACACAACATTTGGTGGAAACGTCTCGACTGCAGCAAATGCTGGTTTTGGAAATGTAAAGGCTGGACATGCATCAACTGCTTTATCTAGGTCAAATGGTTTTATATCGACTAAAGCCAATGGCCCAACCAAGTTTTCCAAGCCTCTCCCTACACAACCTAGCAAGTCACTAAACAAGATTCCTCATCTGGGTAATGATTTCTCAGCGGGTGTTTTGAAGGGATACCCACCAGCTGGAAGATTCTCGTCATTTACCAACCAGAAATATGGTTTGTTTCCTCCTACTGGTCAAACATACTACAAATCTAATGGAAGAATTGTGAATGGAAATGATAGTTTCAAGTTGAGGGAAAAATATAACAGGAATGAAGATTTTGAATCCTCAGCTGAGTTGACACGTGGTCCAAGGTCCCACAACAAGATATCTCCGTTAGACTCACCAATTGAGAAGGAAGAACTAGGAATTAGTGTCCGTAGAGATAAATATAACTTGCCAGATTTTCAGACTGATTATGAAAAAGCAAAGTTTTATGTCATCAAGTCTTACAGTGAAGACGATGTCCATAAGTCCATTAAATATGATGTTTGGGCCAGCACTCCCAATGGCAATAAGAAACTAGATGCAACATTTCATGATGTGGAATTGAAATTGAGGCAGACCAGCACTCAGTGTCCCATCTTCCTCTTCTTCTCAGTAAACGGAAGTGGACAGTTTGTTGGTGTAGCTGAGATGATTGGACAGGTAGATTTTAATAAAGATATGGATTTCTGGCAAGTTGACAAGTGGAGTGGGTTCTTCCCAGTAAAGTGGCATGTAATAAAGGACATACCGAACTCTAAGTTGCGGCACATTATCCTTGAAAACAATGACAACAGACCAGTAACTTTTACCCGGGACACTCAGGAGATTGGCCTCAAGCAAGGTTTGGAAATGCTGAACATTTTTAAAAGTTACACAGCTAAAACGTCTTTGCTAGATGACTTTACGTTCTATGAAAATCGGGAGAAGTCTCTGCAAGCCAAAAGGAGCAATAAACCGGCAACTTTGAAAATGGAAAATTACGATAATGGTGATATCACTAAGCATATGAATGGTGGAGGGAGAAACATCGATGTTGAATCTGGTGGGATGAGGATGGGTTCTCTCATCTCGCTCACGAAAAATCTATCCCTCGACGCCTGCCCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
YTH

Protein Analysis

448

Amino Acids

49.92

Weight (kDa)

8.94

Isoelectric Point (pI)

39.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
YTH PF04146 226 - 368 5.1e-45 YT521-B-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 389
AccBSI CCGCTC 1 cut(s) 56
AciI CCGC 3 cut(s) 56, 362, 1000
AclWI GGATC 1 cut(s) 136
AcoI YGGCCR 1 cut(s) 73
AcvI CACGTG 1 cut(s) 554
AcyI GRCGYC 1 cut(s) 1332
AdeI CACNNNGTG 1 cut(s) 825
AfiI CCNNNNNNNGG 4 cut(s) 98, 344, 389, 440
AflIII ACRYGT 1 cut(s) 551
AgsI TTSAA 7 cut(s) 373, 496, 533, 802, 1016, 1204, 1274
AhdI GACNNNNNGTC 1 cut(s) 555
AjnI CCWGG 2 cut(s) 37, 152
AjuI GAANNNNNNNTTGG 2 cut(s) 274, 306
AluBI AGCT 6 cut(s) 21, 137, 389, 542, 881, 1115
AluI AGCT 6 cut(s) 21, 137, 389, 542, 881, 1115
Alw26I GTCTC 2 cut(s) 184, 1170
AlwI GGATC 1 cut(s) 136
AlwNI CAGNNNCTG 1 cut(s) 137
Ama87I CYCGRG 1 cut(s) 1047
AoxI GGCC 4 cut(s) 73, 277, 744, 1066
ApeKI GCWGC 2 cut(s) 137, 188
ArsI GACNNNNNNTTYG 2 cut(s) 149, 181
AspLEI GCGC 1 cut(s) 142
AspS9I GGNCC 4 cut(s) 278, 557, 564, 744
AsuC2I CCSGG 2 cut(s) 1048, 1049
AsuHPI GGTGA 2 cut(s) 585, 1238
AvaI CYCGRG 1 cut(s) 1047
AvaII GGWCC 2 cut(s) 557, 564
AxyI CCTNAGG 1 cut(s) 97
BalI TGGCCA 1 cut(s) 75
BbrPI CACGTG 1 cut(s) 554
BbsI GAAGAC 1 cut(s) 714
BbvCI CCTCAGC 1 cut(s) 538
BbvI GCAGC 2 cut(s) 124, 200
BccI CCATC 3 cut(s) 43, 839, 1285
BciT130I CCWGG 2 cut(s) 39, 154
BciVI GTATCC 1 cut(s) 371
BcnI CCSGG 2 cut(s) 1048, 1049
BcoDI GTCTC 2 cut(s) 184, 1170
BfaI CTAG 5 cut(s) 243, 315, 614, 773, 1130
BfmI CTRYAG 1 cut(s) 186
BfuI GTATCC 1 cut(s) 371
BisI GCNGC 3 cut(s) 138, 189, 1001
BlsI GCNGC 3 cut(s) 139, 190, 1002
Bme1390I CCNGG 4 cut(s) 39, 154, 1048, 1049
Bme18I GGWCC 2 cut(s) 557, 564
BmeRI GACNNNNNGTC 1 cut(s) 555
BmeT110I CYCGRG 1 cut(s) 1047
BmgT120I GGNCC 4 cut(s) 278, 557, 564, 744
BmiI GGNNCC 1 cut(s) 566
BmrFI CCNGG 4 cut(s) 39, 154, 1048, 1049
BmrI ACTGGG 1 cut(s) 950
BmsI GCATC 2 cut(s) 236, 766
BmuI ACTGGG 1 cut(s) 950
BpiI GAAGAC 1 cut(s) 714
Bpu10I CCTNAGC 1 cut(s) 538
BpuEI CTTGAG 1 cut(s) 1055
BpuMI CCSGG 2 cut(s) 1048, 1049
Bsa29I ATCGAT 1 cut(s) 1266
BsaAI YACGTR 1 cut(s) 554
BsaHI GRCGYC 1 cut(s) 1332
BsaJI CCNNGG 4 cut(s) 37, 153, 560, 1047
Bsc4I CCNNNNNNNGG 4 cut(s) 98, 344, 389, 440
Bse118I RCCGGY 1 cut(s) 1192
Bse1I ACTGG 3 cut(s) 445, 956, 1034
Bse21I CCTNAGG 1 cut(s) 97
BseBI CCWGG 2 cut(s) 39, 154
BseCI ATCGAT 1 cut(s) 1266
BseDI CCNNGG 4 cut(s) 37, 153, 560, 1047
BseGI GGATG 2 cut(s) 1290, 1296
BseLI CCNNNNNNNGG 4 cut(s) 98, 344, 389, 440
BseMII CTCAG 8 cut(s) 111, 372, 534, 552, 836, 861, 873, 1070
BseNI ACTGG 3 cut(s) 445, 956, 1034
BseXI GCAGC 2 cut(s) 124, 200
BseYI CCCAGC 1 cut(s) 17
BshFI GGCC 4 cut(s) 75, 279, 746, 1068
BshVI ATCGAT 1 cut(s) 1266
BsiHKCI CYCGRG 1 cut(s) 1047
BsiSI CCGG 2 cut(s) 1048, 1193
BslFI GGGAC 4 cut(s) 170, 550, 813, 1064
BslI CCNNNNNNNGG 4 cut(s) 98, 344, 389, 440
BsmAI GTCTC 2 cut(s) 184, 1170
BsmBI CGTCTC 1 cut(s) 184
BsmFI GGGAC 4 cut(s) 170, 550, 813, 1064
BsnI GGCC 4 cut(s) 75, 279, 746, 1068
BsoBI CYCGRG 1 cut(s) 1047
Bsp143I GATC 1 cut(s) 128
BspACI CCGC 3 cut(s) 56, 362, 1000
BspANI GGCC 4 cut(s) 75, 279, 746, 1068
BspCNI CTCAG 8 cut(s) 110, 371, 535, 551, 835, 860, 874, 1069
BspDI ATCGAT 1 cut(s) 1266
BspHI TCATGA 1 cut(s) 787
BspLI GGNNCC 1 cut(s) 566
BspMAI CTGCAG 1 cut(s) 190
BspPI GGATC 1 cut(s) 136
BsrBI CCGCTC 1 cut(s) 56
BsrFI RCCGGY 1 cut(s) 1192
BsrI ACTGG 3 cut(s) 445, 956, 1034
BssAI RCCGGY 1 cut(s) 1192
BssECI CCNNGG 4 cut(s) 37, 153, 560, 1047
BssMI GATC 1 cut(s) 128
BssNI GRCGYC 1 cut(s) 1332
BssT1I CCWWGG 1 cut(s) 560
Bst2UI CCWGG 2 cut(s) 39, 154
Bst4CI ACNGT 3 cut(s) 84, 704, 867
Bst6I CTCTTC 1 cut(s) 845
BstACI GRCGYC 1 cut(s) 1332
BstAPI GCANNNNNTGC 2 cut(s) 194, 233
BstBAI YACGTR 1 cut(s) 554
BstC8I GCNNGC 3 cut(s) 748, 1173, 1336
BstENI CCTNNNNNAGG 1 cut(s) 96
BstF5I GGATG 2 cut(s) 1290, 1296
BstHHI GCGC 1 cut(s) 142
BstKTI GATC 1 cut(s) 131
BstMAI GTCTC 2 cut(s) 184, 1170
BstMBI GATC 1 cut(s) 128
BstMWI GCNNNNNNNGC 3 cut(s) 194, 224, 233
BstNI CCWGG 2 cut(s) 39, 154
BstNSI RCATGY 2 cut(s) 227, 971
BstSCI CCNGG 4 cut(s) 37, 152, 1046, 1047
BstSFI CTRYAG 1 cut(s) 186
BstV1I GCAGC 2 cut(s) 124, 200
BstV2I GAAGAC 1 cut(s) 714
BstXI CCANNNNNNTGG 2 cut(s) 422, 963
Bsu15I ATCGAT 1 cut(s) 1266
Bsu36I CCTNAGG 1 cut(s) 97
BsuI GTATCC 1 cut(s) 371
BsuRI GGCC 4 cut(s) 75, 279, 746, 1068
BsuTUI ATCGAT 1 cut(s) 1266
BtsCI GGATG 2 cut(s) 1290, 1296
BtsIMutI CAGTG 2 cut(s) 709, 830
Cac8I GCNNGC 3 cut(s) 748, 1173, 1336
CaiI CAGNNNCTG 1 cut(s) 137
CciI TCATGA 1 cut(s) 787
CfoI GCGC 1 cut(s) 142
Cfr10I RCCGGY 1 cut(s) 1192
Cfr13I GGNCC 4 cut(s) 278, 557, 564, 744
Cfr9I CCCGGG 1 cut(s) 1047
ClaI ATCGAT 1 cut(s) 1266
CspCI CAANNNNNGTGG 2 cut(s) 925, 960
CviAII CATG 3 cut(s) 224, 788, 968
DpnI GATC 1 cut(s) 130
DpnII GATC 1 cut(s) 128
DraI TTTAAA 1 cut(s) 1102
DraIII CACNNNGTG 1 cut(s) 825
DriI GACNNNNNGTC 1 cut(s) 555
EaeI YGGCCR 1 cut(s) 73
Eam1104I CTCTTC 1 cut(s) 845
Eam1105I GACNNNNNGTC 1 cut(s) 555
EarI CTCTTC 1 cut(s) 845
Eco130I CCWWGG 1 cut(s) 560
Eco32I GATATC 2 cut(s) 579, 1231
Eco47I GGWCC 2 cut(s) 557, 564
Eco72I CACGTG 1 cut(s) 554
Eco81I CCTNAGG 1 cut(s) 97
Eco88I CYCGRG 1 cut(s) 1047
EcoNI CCTNNNNNAGG 1 cut(s) 96
EcoO109I RGGNCCY 1 cut(s) 564
EcoRII CCWGG 2 cut(s) 37, 152
EcoRV GATATC 2 cut(s) 579, 1231
EcoT14I CCWWGG 1 cut(s) 560
EcoT22I ATGCAT 1 cut(s) 229
ErhI CCWWGG 1 cut(s) 560
Esp3I CGTCTC 1 cut(s) 184
FaeI CATG 3 cut(s) 227, 791, 971
FaqI GGGAC 4 cut(s) 170, 550, 813, 1064
FatI CATG 3 cut(s) 223, 787, 967
FauI CCCGC 1 cut(s) 355
FauNDI CATATG 3 cut(s) 31, 124, 1242
Fnu4HI GCNGC 3 cut(s) 138, 189, 1001
FokI GGATG 2 cut(s) 1297, 1303
Fsp4HI GCNGC 3 cut(s) 138, 189, 1001
FspBI CTAG 5 cut(s) 243, 315, 614, 773, 1130
GlaI GCGC 1 cut(s) 141
GluI GCNGC 3 cut(s) 138, 189, 1001
GsaI CCCAGC 1 cut(s) 21
HaeIII GGCC 4 cut(s) 75, 279, 746, 1068
HapII CCGG 2 cut(s) 1048, 1193
HhaI GCGC 1 cut(s) 142
Hin1I GRCGYC 1 cut(s) 1332
Hin1II CATG 3 cut(s) 227, 791, 971
Hin6I GCGC 1 cut(s) 140
HinP1I GCGC 1 cut(s) 140
HincII GTYRAC 2 cut(s) 549, 934
HindII GTYRAC 2 cut(s) 549, 934
HinfI GANTC 5 cut(s) 334, 396, 533, 590, 1274
HpaII CCGG 2 cut(s) 1048, 1193
HphI GGTGA 2 cut(s) 585, 1238
Hpy166II GTNNAC 4 cut(s) 549, 853, 863, 934
Hpy188I TCNGA 3 cut(s) 48, 133, 660
Hpy188III TCNNGA 6 cut(s) 99, 181, 788, 1058, 1157, 1312
Hpy8I GTNNAC 4 cut(s) 549, 853, 863, 934
Hpy99I CGWCG 1 cut(s) 1334
HpyAV CCTTC 4 cut(s) 23, 102, 367, 598
HpyCH4III ACNGT 3 cut(s) 84, 704, 867
HpyCH4IV ACGT 4 cut(s) 177, 553, 1121, 1142
HpyCH4V TGCA 5 cut(s) 109, 188, 227, 779, 1171
HpyF10VI GCNNNNNNNGC 3 cut(s) 194, 224, 233
HpySE526I ACGT 4 cut(s) 177, 553, 1121, 1142
Hsp92I GRCGYC 1 cut(s) 1332
Hsp92II CATG 3 cut(s) 227, 791, 971
HspAI GCGC 1 cut(s) 140
Kzo9I GATC 1 cut(s) 128
LmnI GCTCC 2 cut(s) 53, 1182
Lsp1109I GCAGC 2 cut(s) 124, 200
LweI GCATC 2 cut(s) 236, 766
MaeI CTAG 5 cut(s) 243, 315, 614, 773, 1130
MaeII ACGT 4 cut(s) 177, 553, 1121, 1142
MaeIII GTNAC 3 cut(s) 321, 1036, 1106
MalI GATC 1 cut(s) 130
MbiI CCGCTC 1 cut(s) 56
MboI GATC 1 cut(s) 128
MboII GAAGA 9 cut(s) 405, 480, 536, 620, 719, 826, 832, 835, 943
MfeI CAATTG 1 cut(s) 597
MlsI TGGCCA 1 cut(s) 75
MluCI AATT 6 cut(s) 471, 597, 618, 797, 803, 1213
MluNI TGGCCA 1 cut(s) 75
MlyI GAGTC 1 cut(s) 584
Mox20I TGGCCA 1 cut(s) 75
Mph1103I ATGCAT 1 cut(s) 229
MscI TGGCCA 1 cut(s) 75
MseI TTAA 3 cut(s) 729, 906, 1101
MslI CAYNNNNRTG 1 cut(s) 1245
Msp20I TGGCCA 1 cut(s) 75
MspA1I CMGCKG 3 cut(s) 362, 389, 542
MspI CCGG 2 cut(s) 1048, 1193
MspR9I CCNGG 4 cut(s) 39, 154, 1048, 1049
MunI CAATTG 1 cut(s) 597
MvaI CCWGG 2 cut(s) 39, 154
MwoI GCNNNNNNNGC 3 cut(s) 194, 224, 233
NciI CCSGG 2 cut(s) 1048, 1049
NdeI CATATG 3 cut(s) 31, 124, 1242
NdeII GATC 1 cut(s) 128
NlaIII CATG 3 cut(s) 227, 791, 971
NlaIV GGNNCC 1 cut(s) 566
NmuCI GTSAC 1 cut(s) 321
NsiI ATGCAT 1 cut(s) 229
NspI RCATGY 2 cut(s) 227, 971
PagI TCATGA 1 cut(s) 787
PfeI GAWTC 4 cut(s) 334, 396, 533, 1274
PflFI GACNNNGTC 1 cut(s) 713
PflMI CCANNNNNTGG 1 cut(s) 389
PkrI GCNGC 3 cut(s) 139, 190, 1002
PleI GAGTC 1 cut(s) 584
PmaCI CACGTG 1 cut(s) 554
PmlI CACGTG 1 cut(s) 554
PpsI GAGTC 1 cut(s) 584
Ppu21I YACGTR 1 cut(s) 554
PpuMI RGGWCCY 1 cut(s) 564
Psp5II RGGWCCY 1 cut(s) 564
Psp6I CCWGG 2 cut(s) 37, 152
PspCI CACGTG 1 cut(s) 554
PspFI CCCAGC 1 cut(s) 17
PspGI CCWGG 2 cut(s) 37, 152
PspN4I GGNNCC 1 cut(s) 566
PspPI GGNCC 4 cut(s) 278, 557, 564, 744
PspPPI RGGWCCY 1 cut(s) 564
PstI CTGCAG 1 cut(s) 190
PstNI CAGNNNCTG 1 cut(s) 137
PsyI GACNNNGTC 1 cut(s) 713
PvuII CAGCTG 2 cut(s) 389, 542
RseI CAYNNNNRTG 1 cut(s) 1245
SaqAI TTAA 3 cut(s) 729, 906, 1101
SatI GCNGC 3 cut(s) 138, 189, 1001
Sau3AI GATC 1 cut(s) 128
Sau96I GGNCC 4 cut(s) 278, 557, 564, 744
SchI GAGTC 1 cut(s) 584
ScrFI CCNGG 4 cut(s) 39, 154, 1048, 1049
SfaNI GCATC 2 cut(s) 236, 766
SfcI CTRYAG 1 cut(s) 186
SinI GGWCC 2 cut(s) 557, 564
SmaI CCCGGG 1 cut(s) 1049
SmiMI CAYNNNNRTG 1 cut(s) 1245
SmlI CTYRAG 1 cut(s) 1070
SmoI CTYRAG 1 cut(s) 1070
Sse9I AATT 6 cut(s) 471, 597, 618, 797, 803, 1213
SsiI CCGC 3 cut(s) 56, 362, 1000
SspMI CTAG 5 cut(s) 243, 315, 614, 773, 1130
StyD4I CCNGG 4 cut(s) 37, 152, 1046, 1047
StyI CCWWGG 1 cut(s) 560
TaaI ACNGT 3 cut(s) 84, 704, 867
TaiI ACGT 4 cut(s) 180, 556, 1124, 1145
TaqI TCGA 4 cut(s) 182, 263, 1266, 1329
TasI AATT 6 cut(s) 471, 597, 618, 797, 803, 1213
TauI GCSGC 1 cut(s) 1003
TfiI GAWTC 4 cut(s) 334, 396, 533, 1274
Tru1I TTAA 3 cut(s) 729, 906, 1101
Tru9I TTAA 3 cut(s) 729, 906, 1101
TscAI CASTG 2 cut(s) 709, 830
TseFI GTSAC 1 cut(s) 321
TseI GCWGC 2 cut(s) 137, 188
Tsp45I GTSAC 1 cut(s) 321
TspDTI ATGAA 6 cut(s) 111, 537, 684, 776, 1164, 1259
TspGWI ACGGA 3 cut(s) 573, 617, 870
TspMI CCCGGG 1 cut(s) 1047
TspRI CASTG 2 cut(s) 709, 830
Tth111I GACNNNGTC 1 cut(s) 713
Van91I CCANNNNNTGG 1 cut(s) 389
VpaK11BI GGWCC 2 cut(s) 557, 564
XagI CCTNNNNNAGG 1 cut(s) 96
XceI RCATGY 2 cut(s) 227, 971
XmaI CCCGGG 1 cut(s) 1047
XspI CTAG 5 cut(s) 243, 315, 614, 773, 1130
Zsp2I ATGCAT 1 cut(s) 229
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.