Basic Information

Type: gene

Biological Identity

Species

rosa_multiflora

Chromosome

Rmu_sc0000307.1

Orthogroup ID

OG0004199

Physical Location & Seq

Genomic Coordinates Forward (+)

26507 .. 31805

Gene Structure 5299 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rmu_sc0000307.1_g000004.1.cds

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1614 bp

atggccggcgaaaagaagattgagaaagctgagcctgtagccactgtgttggcatcagattctgtttccggattaggagagcaacaactggtttctgggaaagatggaataccatatgattcgatctcctccatgtcttcttcgagggatgccaccgacagcatgaaaggatataatggatcgtatacgcagatggacgaacatcgctatgcaaatgctaataattcacaggcgggtgtgcagtcagacaatggttcaatggtctattatctccccggctataatccatatgcccctggaactcttatgggtgttgatggccaaggtgttggtcaacagcagtatttttcttcctcaggttatatgcagccacctgtttcgtatggttctgaagctatgccctgttattcatgggacacaacatattctggtgatatctctacagcaacaaactctggttttggaaatctaaagtctggaccaggtggtactgctttatctaggtcaagtggctttacgtcaggcaaaacaaatggaaatcttgctagcaaattttccaagtctttcccttctacacaacccatcaaatcattaaacaaggtgcctcatttaggttctgatttctcagccagtcttttgaagggatacccctcaactggaaggttctcatcattcactaaccagaagggtctgtttcctcttaatggtcaaacaaactacaaatctaatgggaggatattaaatggaaatgacagattcaagtcaagggaaaaatataacagaaatgaggattttgaatcctcaactgagttgacacgtggtccaagatctcgcaacaagatttctccttcagactcatcagtggagaaggaagtattgggatttacagtcagtagagataaatacaacctaccagattttcagactgattatgaaagtgcgaagttctatgtcattaagtcttacagtgaagatgatgtccataagagcattaaatatgatgtttgggccagcactccgaatggcaataagaaactggatgtagcatttcgtgatgctgaagcaaaatcgagtgagacaggcacacggtgtcctatattcctatttttctcggtgaatggaagtgggcagttcgtaggcctggccgagatgattggggaggtagatttcaacaaagatatggacttctggcaagttgacaagtggagtgggttcttcccagttaaatggcatataataaaggacatacctaacactcagttgcggcatattatccttgaaaacaatgataacagacctgtaacttttacccgggacactcaagagattggactgaagcaaggtttggaaatgctgaacatattcaaaacttatgcacccagatcatctttgttagatgacttcaatttctatgaaaatcgagaaaagtctctacaagccaaaaggagcggtaaacctgcaactttaaagattgaaacttacgatgatggtgttatccctgagcatatgagtgctgtaggggaagaccttgaagctgaatcaggtgggatcgggatgacttctgaccgaacatctctcatttcacttactaaaaatctctctctcaatgcttga

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

YTH

Protein Analysis

537

Amino Acids

59.07

Weight (kDa)

5.85

Isoelectric Point (pI)

37.52

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004199)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G09810 AT1G09810 AT1G09810
fragaria_vesca	FvH4_5g30420 FvH4_5g30420 FvH4_5g30420 FvH4_5g30420 FvH4_5g30420 FvH4_5g30420 FvH4_5g30420
malus_domestica	MD08G1241700.v1.1 MD15G1435500.v1.1
prunus_persica	Prupe.1G579100_v2.0.a1 Prupe.1G579100_v2.0.a1 Prupe.1G579100_v2.0.a1 Prupe.1G579100_v2.0.a1 Prupe.1G579100_v2.0.a1 Prupe.1G579100_v2.0.a1 Prupe.1G579100_v2.0.a1 Prupe.1G579100_v2.0.a1
pyrus_communis	pycom08g20970 pycom15g38420
rosa_chinensis	RchiOBHm_Chr7g0224861
rosa_laevigata	RLG00000001848
rosa_multiflora	Rmu_sc0000307.1_g000004
rosa_roxburghii	Rroxscaffold_3G00234320
rosa_rugosa	Rorug07G0226600 Rorug07G0226600
rosa_samantha	Rh7AG376800 Rh7BG362100 Rh7CG395300 Rh7DG372100
rosa_wichuraiana	Rw7G031390

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1465
AccB1I	GGYRCC	1 cut(s)	601
AccBSI	CCGCTC	1 cut(s)	1449
AccI	GTMKAC	1 cut(s)	185
AccIII	TCCGGA	1 cut(s)	68
AciI	CCGC	3 cut(s)	233, 1264, 1449
AclWI	GGATC	2 cut(s)	187, 1556
AcoI	YGGCCR	3 cut(s)	3, 319, 1143
AcsI	RAATTY	1 cut(s)	551
AcuI	CTGAAG	4 cut(s)	411, 834, 1080, 1355
AcvI	CACGTG	1 cut(s)	818
AdeI	CACNNNGTG	1 cut(s)	1089
AfaI	GTAC	1 cut(s)	490
AfiI	CCNNNNNNNGG	3 cut(s)	611, 656, 704
AflIII	ACRYGT	1 cut(s)	815
AhdI	GACNNNNNGTC	1 cut(s)	819
AjnI	CCWGG	3 cut(s)	295, 481, 1140
AjuI	GAANNNNNNNTTGG	4 cut(s)	860, 892, 1328, 1360
AluBI	AGCT	3 cut(s)	29, 395, 1535
AluI	AGCT	3 cut(s)	29, 395, 1535
Alw26I	GTCTC	2 cut(s)	1070, 1434
AlwI	GGATC	2 cut(s)	187, 1556
AlwNI	CAGNNNCTG	1 cut(s)	62
Ama87I	CYCGRG	1 cut(s)	1311
Aor13HI	TCCGGA	1 cut(s)	68
AoxI	GGCC	5 cut(s)	3, 319, 1008, 1138, 1143
ApeKI	GCWGC	1 cut(s)	367
ApoI	RAATTY	1 cut(s)	551
Asp700I	GAANNNNTTC	1 cut(s)	1361
AspS9I	GGNCC	3 cut(s)	479, 821, 1008
AsuC2I	CCSGG	3 cut(s)	276, 1312, 1313
AsuHPI	GGTGA	2 cut(s)	443, 1126
AsuNHI	GCTAGC	1 cut(s)	545
AvaI	CYCGRG	1 cut(s)	1311
AvaII	GGWCC	2 cut(s)	479, 821
AxyI	CCTNAGG	1 cut(s)	355
BalI	TGGCCA	1 cut(s)	321
BanI	GGYRCC	1 cut(s)	601
BbrPI	CACGTG	1 cut(s)	818
BbsI	GAAGAC	2 cut(s)	129, 1530
BbvI	GCAGC	1 cut(s)	379
BccI	CCATC	5 cut(s)	98, 187, 311, 590, 1481
BciT130I	CCWGG	3 cut(s)	297, 483, 1142
BciVI	GTATCC	1 cut(s)	638
BcnI	CCSGG	3 cut(s)	276, 1312, 1313
BcoDI	GTCTC	2 cut(s)	1070, 1434
BfaI	CTAG	2 cut(s)	501, 546
BfmI	CTRYAG	3 cut(s)	36, 441, 1515
BfuAI	ACCTGC	1 cut(s)	1465
BfuI	GTATCC	1 cut(s)	638
BglII	AGATCT	1 cut(s)	827
BisI	GCNGC	2 cut(s)	368, 1265
BlpI	GCTNAGC	1 cut(s)	30
BlsI	GCNGC	2 cut(s)	369, 1266
Bme1390I	CCNGG	6 cut(s)	276, 297, 483, 1142, 1312, 1313
Bme18I	GGWCC	2 cut(s)	479, 821
BmeRI	GACNNNNNGTC	1 cut(s)	819
BmeT110I	CYCGRG	1 cut(s)	1311
BmgT120I	GGNCC	3 cut(s)	479, 821, 1008
BmiI	GGNNCC	1 cut(s)	603
BmrFI	CCNGG	6 cut(s)	276, 297, 483, 1142, 1312, 1313
BmrI	ACTGGG	1 cut(s)	1214
BmsI	GCATC	3 cut(s)	62, 139, 1045
BmtI	GCTAGC	1 cut(s)	549
BmuI	ACTGGG	1 cut(s)	1214
BpiI	GAAGAC	2 cut(s)	129, 1530
Bpu10I	CCTNAGC	1 cut(s)	1500
Bpu1102I	GCTNAGC	1 cut(s)	30
BpuEI	CTTGAG	1 cut(s)	1305
BpuMI	CCSGG	3 cut(s)	276, 1312, 1313
BsaAI	YACGTR	1 cut(s)	818
BsaJI	CCNNGG	4 cut(s)	274, 295, 322, 1311
BsaWI	WCCGGW	1 cut(s)	68
Bsc4I	CCNNNNNNNGG	3 cut(s)	611, 656, 704
Bse118I	RCCGGY	1 cut(s)	5
Bse1I	ACTGG	5 cut(s)	93, 630, 661, 1041, 1220
Bse21I	CCTNAGG	1 cut(s)	355
BseAI	TCCGGA	1 cut(s)	68
BseBI	CCWGG	3 cut(s)	297, 483, 1142
BseDI	CCNNGG	4 cut(s)	274, 295, 322, 1311
BseGI	GGATG	3 cut(s)	154, 1045, 1560
BseLI	CCNNNNNNNGG	3 cut(s)	611, 656, 704
BseMII	CTCAG	6 cut(s)	21, 369, 639, 798, 1271, 1491
BseNI	ACTGG	5 cut(s)	93, 630, 661, 1041, 1220
BseRI	GAGGAG	1 cut(s)	118
BseXI	GCAGC	1 cut(s)	379
BsgI	GTGCAG	1 cut(s)	260
BshFI	GGCC	5 cut(s)	5, 321, 1010, 1140, 1145
BshNI	GGYRCC	1 cut(s)	601
BsiHKCI	CYCGRG	1 cut(s)	1311
BsiSI	CCGG	4 cut(s)	6, 69, 276, 1312
BslFI	GGGAC	2 cut(s)	428, 1328
BslI	CCNNNNNNNGG	3 cut(s)	611, 656, 704
BsmAI	GTCTC	2 cut(s)	1070, 1434
BsmFI	GGGAC	2 cut(s)	428, 1328
BsnI	GGCC	5 cut(s)	5, 321, 1010, 1140, 1145
BsoBI	CYCGRG	1 cut(s)	1311
Bsp13I	TCCGGA	1 cut(s)	68
Bsp143I	GATC	5 cut(s)	123, 179, 827, 1382, 1548
Bsp1720I	GCTNAGC	1 cut(s)	30
BspACI	CCGC	3 cut(s)	233, 1264, 1449
BspANI	GGCC	5 cut(s)	5, 321, 1010, 1140, 1145
BspCNI	CTCAG	6 cut(s)	22, 368, 638, 799, 1270, 1492
BspEI	TCCGGA	1 cut(s)	68
BspLI	GGNNCC	1 cut(s)	603
BspMI	ACCTGC	1 cut(s)	1465
BspOI	GCTAGC	1 cut(s)	549
BspPI	GGATC	2 cut(s)	187, 1556
BspT107I	GGYRCC	1 cut(s)	601
BsrBI	CCGCTC	1 cut(s)	1449
BsrFI	RCCGGY	1 cut(s)	5
BsrI	ACTGG	5 cut(s)	93, 630, 661, 1041, 1220
BssAI	RCCGGY	1 cut(s)	5
BssECI	CCNNGG	4 cut(s)	274, 295, 322, 1311
BssMI	GATC	5 cut(s)	123, 179, 827, 1382, 1548
BssNAI	GTATAC	1 cut(s)	186
BssT1I	CCWWGG	1 cut(s)	322
Bst1107I	GTATAC	1 cut(s)	186
Bst2UI	CCWGG	3 cut(s)	297, 483, 1142
Bst4CI	ACNGT	4 cut(s)	46, 889, 968, 1089
BstBAI	YACGTR	1 cut(s)	818
BstC8I	GCNNGC	3 cut(s)	7, 547, 1012
BstDEI	CTNAG	6 cut(s)	30, 355, 625, 807, 1257, 1500
BstENI	CCTNNNNNAGG	1 cut(s)	609
BstF5I	GGATG	3 cut(s)	154, 1045, 1560
BstKTI	GATC	5 cut(s)	126, 182, 830, 1385, 1551
BstMAI	GTCTC	2 cut(s)	1070, 1434
BstMBI	GATC	5 cut(s)	123, 179, 827, 1382, 1548
BstNI	CCWGG	3 cut(s)	297, 483, 1142
BstSCI	CCNGG	6 cut(s)	274, 295, 481, 1140, 1310, 1311
BstSFI	CTRYAG	3 cut(s)	36, 441, 1515
BstV1I	GCAGC	1 cut(s)	379
BstV2I	GAAGAC	2 cut(s)	129, 1530
BstX2I	RGATCY	1 cut(s)	827
BstXI	CCANNNNNNTGG	3 cut(s)	49, 329, 1227
BstYI	RGATCY	1 cut(s)	827
BstZ17I	GTATAC	1 cut(s)	186
Bsu36I	CCTNAGG	1 cut(s)	355
BsuI	GTATCC	1 cut(s)	638
BsuRI	GGCC	5 cut(s)	5, 321, 1010, 1140, 1145
BtgZI	GCGATG	1 cut(s)	188
BtsCI	GGATG	3 cut(s)	154, 1045, 1560
BtsIMutI	CAGTG	3 cut(s)	42, 867, 973
BveI	ACCTGC	1 cut(s)	1465
Cac8I	GCNNGC	3 cut(s)	7, 547, 1012
CaiI	CAGNNNCTG	1 cut(s)	62
Cfr10I	RCCGGY	1 cut(s)	5
Cfr13I	GGNCC	3 cut(s)	479, 821, 1008
Cfr9I	CCCGGG	1 cut(s)	1311
CsiI	ACCWGGT	1 cut(s)	481
Csp6I	GTAC	1 cut(s)	489
CspCI	CAANNNNNGTGG	2 cut(s)	1189, 1224
CviAII	CATG	3 cut(s)	133, 163, 411
CviQI	GTAC	1 cut(s)	489
DdeI	CTNAG	6 cut(s)	30, 355, 625, 807, 1257, 1500
DpnI	GATC	5 cut(s)	125, 181, 829, 1384, 1550
DpnII	GATC	5 cut(s)	123, 179, 827, 1382, 1548
DraI	TTTAAA	1 cut(s)	1467
DraIII	CACNNNGTG	1 cut(s)	1089
DriI	GACNNNNNGTC	1 cut(s)	819
EaeI	YGGCCR	3 cut(s)	3, 319, 1143
Eam1105I	GACNNNNNGTC	1 cut(s)	819
Eco130I	CCWWGG	1 cut(s)	322
Eco147I	AGGCCT	1 cut(s)	1140
Eco32I	GATATC	1 cut(s)	436
Eco47I	GGWCC	2 cut(s)	479, 821
Eco57I	CTGAAG	4 cut(s)	411, 834, 1080, 1355
Eco72I	CACGTG	1 cut(s)	818
Eco81I	CCTNAGG	1 cut(s)	355
Eco88I	CYCGRG	1 cut(s)	1311
EcoNI	CCTNNNNNAGG	1 cut(s)	609
EcoRII	CCWGG	3 cut(s)	295, 481, 1140
EcoRV	GATATC	1 cut(s)	436
EcoT14I	CCWWGG	1 cut(s)	322
ErhI	CCWWGG	1 cut(s)	322
FaeI	CATG	3 cut(s)	136, 166, 414
FaqI	GGGAC	2 cut(s)	428, 1328
FatI	CATG	3 cut(s)	132, 162, 410
FauI	CCCGC	1 cut(s)	226
FauNDI	CATATG	3 cut(s)	115, 289, 1506
FblI	GTMKAC	1 cut(s)	185
Fnu4HI	GCNGC	2 cut(s)	368, 1265
FokI	GGATG	3 cut(s)	161, 1052, 1567
Fsp4HI	GCNGC	2 cut(s)	368, 1265
FspBI	CTAG	2 cut(s)	501, 546
GluI	GCNGC	2 cut(s)	368, 1265
HaeIII	GGCC	5 cut(s)	5, 321, 1010, 1140, 1145
HapII	CCGG	4 cut(s)	6, 69, 276, 1312
Hin1II	CATG	3 cut(s)	136, 166, 414
HincII	GTYRAC	3 cut(s)	335, 813, 1198
HindII	GTYRAC	3 cut(s)	335, 813, 1198
HinfI	GANTC	6 cut(s)	59, 119, 756, 797, 854, 1538
HpaII	CCGG	4 cut(s)	6, 69, 276, 1312
HphI	GGTGA	2 cut(s)	443, 1126
Hpy166II	GTNNAC	5 cut(s)	186, 335, 813, 1198, 1454
Hpy188I	TCNGA	8 cut(s)	58, 247, 391, 619, 853, 924, 1020, 1564
Hpy188III	TCNNGA	6 cut(s)	69, 477, 1052, 1322, 1421, 1552
Hpy8I	GTNNAC	5 cut(s)	186, 335, 813, 1198, 1454
HpyAV	CCTTC	6 cut(s)	579, 634, 654, 679, 858, 862
HpyCH4III	ACNGT	4 cut(s)	46, 889, 968, 1089
HpyCH4IV	ACGT	2 cut(s)	518, 817
HpyCH4V	TGCA	5 cut(s)	212, 241, 367, 1376, 1460
HpyF3I	CTNAG	6 cut(s)	30, 355, 625, 807, 1257, 1500
HpySE526I	ACGT	2 cut(s)	518, 817
Hsp92II	CATG	3 cut(s)	136, 166, 414
Kpn2I	TCCGGA	1 cut(s)	68
KroI	GCCGGC	1 cut(s)	5
KroNI	GCCGGC	1 cut(s)	7
Kzo9I	GATC	5 cut(s)	123, 179, 827, 1382, 1548
LmnI	GCTCC	1 cut(s)	1446
Lsp1109I	GCAGC	1 cut(s)	379
LweI	GCATC	3 cut(s)	62, 139, 1045
MabI	ACCWGGT	1 cut(s)	481
MaeI	CTAG	2 cut(s)	501, 546
MaeII	ACGT	2 cut(s)	518, 817
MaeIII	GTNAC	1 cut(s)	1300
MalI	GATC	5 cut(s)	125, 181, 829, 1384, 1550
MbiI	CCGCTC	1 cut(s)	1449
MboI	GATC	5 cut(s)	123, 179, 827, 1382, 1548
MboII	GAAGA	7 cut(s)	28, 129, 132, 342, 983, 1207, 1535
MflI	RGATCY	1 cut(s)	827
MlsI	TGGCCA	1 cut(s)	321
MluCI	AATT	3 cut(s)	223, 551, 1405
MluNI	TGGCCA	1 cut(s)	321
MlyI	GAGTC	1 cut(s)	848
Mox20I	TGGCCA	1 cut(s)	321
MroI	TCCGGA	1 cut(s)	68
MroNI	GCCGGC	1 cut(s)	5
MroXI	GAANNNNTTC	1 cut(s)	1361
MscI	TGGCCA	1 cut(s)	321
MseI	TTAA	7 cut(s)	593, 702, 740, 957, 993, 1224, 1466
MslI	CAYNNNNRTG	2 cut(s)	207, 1509
Msp20I	TGGCCA	1 cut(s)	321
MspI	CCGG	4 cut(s)	6, 69, 276, 1312
MspR9I	CCNGG	6 cut(s)	276, 297, 483, 1142, 1312, 1313
MvaI	CCWGG	3 cut(s)	297, 483, 1142
NaeI	GCCGGC	1 cut(s)	7
NciI	CCSGG	3 cut(s)	276, 1312, 1313
NdeI	CATATG	3 cut(s)	115, 289, 1506
NdeII	GATC	5 cut(s)	123, 179, 827, 1382, 1548
NgoMIV	GCCGGC	1 cut(s)	5
NheI	GCTAGC	1 cut(s)	545
NlaIII	CATG	3 cut(s)	136, 166, 414
NlaIV	GGNNCC	1 cut(s)	603
NmeAIII	GCCGAG	1 cut(s)	1171
PceI	AGGCCT	1 cut(s)	1140
PdiI	GCCGGC	1 cut(s)	7
PdmI	GAANNNNTTC	1 cut(s)	1361
PfeI	GAWTC	5 cut(s)	59, 119, 756, 797, 1538
PkrI	GCNGC	2 cut(s)	369, 1266
PleI	GAGTC	1 cut(s)	848
PmaCI	CACGTG	1 cut(s)	818
PmlI	CACGTG	1 cut(s)	818
PpsI	GAGTC	1 cut(s)	848
Ppu21I	YACGTR	1 cut(s)	818
Psp6I	CCWGG	3 cut(s)	295, 481, 1140
PspCI	CACGTG	1 cut(s)	818
PspGI	CCWGG	3 cut(s)	295, 481, 1140
PspN4I	GGNNCC	1 cut(s)	603
PspPI	GGNCC	3 cut(s)	479, 821, 1008
PstNI	CAGNNNCTG	1 cut(s)	62
PsuI	RGATCY	1 cut(s)	827
RsaI	GTAC	1 cut(s)	490
RsaNI	GTAC	1 cut(s)	489
RseI	CAYNNNNRTG	2 cut(s)	207, 1509
SaqAI	TTAA	7 cut(s)	593, 702, 740, 957, 993, 1224, 1466
SatI	GCNGC	2 cut(s)	368, 1265
Sau3AI	GATC	5 cut(s)	123, 179, 827, 1382, 1548
Sau96I	GGNCC	3 cut(s)	479, 821, 1008
SchI	GAGTC	1 cut(s)	848
ScrFI	CCNGG	6 cut(s)	276, 297, 483, 1142, 1312, 1313
SexAI	ACCWGGT	1 cut(s)	481
SfaNI	GCATC	3 cut(s)	62, 139, 1045
SfcI	CTRYAG	3 cut(s)	36, 441, 1515
SinI	GGWCC	2 cut(s)	479, 821
SmaI	CCCGGG	1 cut(s)	1313
SmiMI	CAYNNNNRTG	2 cut(s)	207, 1509
SmlI	CTYRAG	1 cut(s)	1320
SmoI	CTYRAG	1 cut(s)	1320
Sse9I	AATT	3 cut(s)	223, 551, 1405
SseBI	AGGCCT	1 cut(s)	1140
SsiI	CCGC	3 cut(s)	233, 1264, 1449
SspMI	CTAG	2 cut(s)	501, 546
StuI	AGGCCT	1 cut(s)	1140
StyD4I	CCNGG	6 cut(s)	274, 295, 481, 1140, 1310, 1311
StyI	CCWWGG	1 cut(s)	322
TaaI	ACNGT	4 cut(s)	46, 889, 968, 1089
TaiI	ACGT	2 cut(s)	521, 820
TaqI	TCGA	4 cut(s)	122, 143, 1070, 1420
TaqII	GACCGA	1 cut(s)	1581
TasI	AATT	3 cut(s)	223, 551, 1405
TauI	GCSGC	1 cut(s)	1267
TfiI	GAWTC	5 cut(s)	59, 119, 756, 797, 1538
Tru1I	TTAA	7 cut(s)	593, 702, 740, 957, 993, 1224, 1466
Tru9I	TTAA	7 cut(s)	593, 702, 740, 957, 993, 1224, 1466
TscAI	CASTG	3 cut(s)	49, 867, 973
TseI	GCWGC	1 cut(s)	367
TspDTI	ATGAA	4 cut(s)	179, 399, 948, 1428
TspMI	CCCGGG	1 cut(s)	1311
TspRI	CASTG	3 cut(s)	49, 867, 973
VpaK11BI	GGWCC	2 cut(s)	479, 821
XagI	CCTNNNNNAGG	1 cut(s)	609
XapI	RAATTY	1 cut(s)	551
XmaI	CCCGGG	1 cut(s)	1311
XmiI	GTMKAC	1 cut(s)	185
XmnI	GAANNNNTTC	1 cut(s)	1361
XspI	CTAG	2 cut(s)	501, 546

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.