RchiOBHm_Chr7g0224861

YT521-B-like domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0004199
Physical Location & Seq
Forward (+)
47259841 .. 47265719
5879 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ20135

Sequence Viewer

Length: 1689 bp
ATGGCCGGCGAAAAGAAGATTGAGAAAGCTGAGCCTGTAGCCACTGTGTTGGCATCAGATTCTGTTTCCGGATTAGGAGAGCAACAGCGGGTTTCTGGGAAAGATGGAATACCATATGATTCGATCTCCTCCATGTCTTCTTCGAGGGATGCCACCGACAGCATGAAAGGTGAGACTGATCAAGAGTCGGTTGGAGAGCATGGTGTTTACTATCCACCCACTAGCTGTTACAATTATTATTATCCAGGATATAATGGATCCTATACGCAGATGGACGAACATCGCTATGCTAATGCTAATAATTCACAGGCGGGTGTGCAGTCAGACAATGGTTCAATGGTCTATTATCTCCCCGGCTATAATCCATATGCCCCTGGAACTCTTATGGGTGTTGATGGCCAAGGTGTTGGTCAACAGCAGTATTTTTCTTCCTCAGGTTATATGCAGCCACCTTCGTATGGTTCTGAAGCTATGCCTTGTTATCCATGGGACACAACATATTCTGGTGATATCTCTACAGCAACAAACTCTGGTTTTGGAAATCTAAAGTCTGGACCAGGTGGTACTGCTTTATCTAGGTCAAGTGGCTTTATGTCGGGCAAAACAAATGGAAATCTTGCTAGCAAATTTTCCAAGTCTTTCCCTTCTACACAACCCATCAAATCATTAAACAAGGTGCCTCATTTAGGTTCTGATTTCTCAGCCAGTCTTTTGAAGGGATACCCCTCAGCTGGAAGGTTCTCATCATTCACTAACCAGAAGGGTTTGTTTCCTCTTAATGGTCAAACAAACTACAAATCTAATGGGAGGATATTAAATGGAAACGACAGATTCAAGTCAAGGGAAAAATATAACAGAAATGAGGATTTTGAATCCTCAACTGAGTTGACACGTGGTCCAAGATCTCGCAACAAGATTACTCCTTCAGACTCATCAGTTGAGAAGGAAGTATTGGGATTTACAGTCAGTAGAGATAAATACAACCTACCAGATTTTCGGACTGATTATGAAAGTGCGAAGTTCTATGTCATTAAGTCTTACAGTGAAGATGATGTCCATAAGAGCATTAAATATGATGTTTGGGCCAGCACTCCGAATGGCAATAAGAAACTGGATGTAGCATTTCGTGATGCTGAAGCAAAATCGAGTGAGACAGGCACGCGGTGTCCTATATTCCTATTTTTCTCGGTGAATGGAAGTGGGCAGTTTGTAGGCCTGGCCGAGATGATTGGGGAGGTAGATTTCAACAAAGATATGGACTTCTGGCAAGTTGACAAGTGGAGTGGGTTCTTCCCAGTTAAATGGCATATAATAAAGGACATACCTAACACTCAGTTGCGGCATATTATCCTTGAAAACAATGATAACAGACCTGTAACTTTTACCCGGGACACTCAAGAGATTGGACTGAAGCAAGGTTTGGAAATGCTGAACATATTCAAAACTTATGCACCCAGATCATCTTTGTTAGATGACTTCAATTTCTATGAAAATCGAGAAAAGTCTCTACAAGCCAAAAGGAGCAGTAAACCTGCAACTTTAAAGATTGAAACTTACGATGATGGTGTTATCCCTGAGCATATGAGTGCCGTAGGGGAAGACCTTGAAGCTGAATCAGGTGGGATCGGGATGACTTCTGACCGAACATCTCTCATTTCACTTACTAAAAATCTCTCTCTCAATGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
YTH

Protein Analysis

562

Amino Acids

62.1

Weight (kDa)

5.7

Isoelectric Point (pI)

38.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
YTH PF04146 339 - 481 2.7e-45 YT521-B-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1540
AccB1I GGYRCC 1 cut(s) 676
AccII CGCG 1 cut(s) 1162
AccIII TCCGGA 1 cut(s) 68
AciI CCGC 4 cut(s) 88, 311, 1162, 1339
AclWI GGATC 3 cut(s) 252, 265, 1631
AcoI YGGCCR 3 cut(s) 3, 397, 1218
AcsI RAATTY 1 cut(s) 626
AcuI CTGAAG 4 cut(s) 486, 909, 1155, 1430
AcvI CACGTG 1 cut(s) 893
AdeI CACNNNGTG 1 cut(s) 1164
AfaI GTAC 1 cut(s) 565
AfiI CCNNNNNNNGG 4 cut(s) 458, 686, 731, 779
AflIII ACRYGT 1 cut(s) 890
AhdI GACNNNNNGTC 1 cut(s) 894
AjnI CCWGG 4 cut(s) 244, 373, 556, 1215
AjuI GAANNNNNNNTTGG 4 cut(s) 935, 967, 1403, 1435
AluBI AGCT 5 cut(s) 29, 225, 470, 731, 1610
AluI AGCT 5 cut(s) 29, 225, 470, 731, 1610
Alw26I GTCTC 3 cut(s) 167, 1145, 1509
AlwI GGATC 3 cut(s) 252, 265, 1631
AlwNI CAGNNNCTG 1 cut(s) 62
Ama87I CYCGRG 1 cut(s) 1386
Aor13HI TCCGGA 1 cut(s) 68
AoxI GGCC 5 cut(s) 3, 397, 1083, 1213, 1218
ApeKI GCWGC 1 cut(s) 445
ApoI RAATTY 1 cut(s) 626
Asp700I GAANNNNTTC 1 cut(s) 1436
AspS9I GGNCC 3 cut(s) 554, 896, 1083
AsuC2I CCSGG 3 cut(s) 354, 1387, 1388
AsuHPI GGTGA 3 cut(s) 182, 518, 1201
AsuNHI GCTAGC 1 cut(s) 620
AvaI CYCGRG 1 cut(s) 1386
AvaII GGWCC 2 cut(s) 554, 896
AxyI CCTNAGG 1 cut(s) 433
BalI TGGCCA 1 cut(s) 399
BamHI GGATCC 1 cut(s) 257
BanI GGYRCC 1 cut(s) 676
BbrPI CACGTG 1 cut(s) 893
BbsI GAAGAC 2 cut(s) 129, 1605
BbvCI CCTCAGC 1 cut(s) 727
BbvI GCAGC 1 cut(s) 457
BccI CCATC 5 cut(s) 98, 265, 389, 665, 1556
BceAI ACGGC 1 cut(s) 1574
BciT130I CCWGG 4 cut(s) 246, 375, 558, 1217
BciVI GTATCC 1 cut(s) 713
BclI TGATCA 1 cut(s) 178
BcnI CCSGG 3 cut(s) 354, 1387, 1388
BcoDI GTCTC 3 cut(s) 167, 1145, 1509
BfaI CTAG 3 cut(s) 222, 576, 621
BfmI CTRYAG 2 cut(s) 36, 516
BfuAI ACCTGC 1 cut(s) 1540
BfuI GTATCC 1 cut(s) 713
BglII AGATCT 1 cut(s) 902
BisI GCNGC 2 cut(s) 446, 1340
BlpI GCTNAGC 1 cut(s) 30
BlsI GCNGC 2 cut(s) 447, 1341
Bme1390I CCNGG 7 cut(s) 246, 354, 375, 558, 1217, 1387, 1388
Bme18I GGWCC 2 cut(s) 554, 896
BmeRI GACNNNNNGTC 1 cut(s) 894
BmeT110I CYCGRG 1 cut(s) 1386
BmgT120I GGNCC 3 cut(s) 554, 896, 1083
BmiI GGNNCC 2 cut(s) 259, 678
BmrFI CCNGG 7 cut(s) 246, 354, 375, 558, 1217, 1387, 1388
BmrI ACTGGG 1 cut(s) 1289
BmsI GCATC 3 cut(s) 62, 139, 1120
BmtI GCTAGC 1 cut(s) 624
BmuI ACTGGG 1 cut(s) 1289
BpiI GAAGAC 2 cut(s) 129, 1605
Bpu10I CCTNAGC 2 cut(s) 727, 1575
Bpu1102I GCTNAGC 1 cut(s) 30
BpuEI CTTGAG 1 cut(s) 1380
BpuMI CCSGG 3 cut(s) 354, 1387, 1388
BsaAI YACGTR 1 cut(s) 893
BsaJI CCNNGG 5 cut(s) 352, 373, 400, 485, 1386
BsaWI WCCGGW 1 cut(s) 68
Bsc4I CCNNNNNNNGG 4 cut(s) 458, 686, 731, 779
Bse118I RCCGGY 1 cut(s) 5
Bse1I ACTGG 3 cut(s) 705, 1116, 1295
Bse21I CCTNAGG 1 cut(s) 433
BseAI TCCGGA 1 cut(s) 68
BseBI CCWGG 4 cut(s) 246, 375, 558, 1217
BseDI CCNNGG 5 cut(s) 352, 373, 400, 485, 1386
BseGI GGATG 3 cut(s) 154, 1120, 1635
BseLI CCNNNNNNNGG 4 cut(s) 458, 686, 731, 779
BseMII CTCAG 7 cut(s) 21, 447, 714, 741, 873, 1346, 1566
BseNI ACTGG 3 cut(s) 705, 1116, 1295
BseRI GAGGAG 1 cut(s) 118
BseXI GCAGC 1 cut(s) 457
BsgI GTGCAG 1 cut(s) 338
Bsh1236I CGCG 1 cut(s) 1162
BshFI GGCC 5 cut(s) 5, 399, 1085, 1215, 1220
BshNI GGYRCC 1 cut(s) 676
BsiHKCI CYCGRG 1 cut(s) 1386
BsiSI CCGG 4 cut(s) 6, 69, 354, 1387
BslFI GGGAC 2 cut(s) 503, 1403
BslI CCNNNNNNNGG 4 cut(s) 458, 686, 731, 779
BsmAI GTCTC 3 cut(s) 167, 1145, 1509
BsmFI GGGAC 2 cut(s) 503, 1403
BsnI GGCC 5 cut(s) 5, 399, 1085, 1215, 1220
BsoBI CYCGRG 1 cut(s) 1386
Bsp13I TCCGGA 1 cut(s) 68
Bsp143I GATC 6 cut(s) 123, 178, 257, 902, 1457, 1623
Bsp1720I GCTNAGC 1 cut(s) 30
Bsp19I CCATGG 1 cut(s) 485
BspACI CCGC 4 cut(s) 88, 311, 1162, 1339
BspANI GGCC 5 cut(s) 5, 399, 1085, 1215, 1220
BspCNI CTCAG 7 cut(s) 22, 446, 713, 740, 874, 1345, 1567
BspEI TCCGGA 1 cut(s) 68
BspFNI CGCG 1 cut(s) 1162
BspLI GGNNCC 2 cut(s) 259, 678
BspMI ACCTGC 1 cut(s) 1540
BspOI GCTAGC 1 cut(s) 624
BspPI GGATC 3 cut(s) 252, 265, 1631
BspT107I GGYRCC 1 cut(s) 676
BsrFI RCCGGY 1 cut(s) 5
BsrI ACTGG 3 cut(s) 705, 1116, 1295
BssAI RCCGGY 1 cut(s) 5
BssECI CCNNGG 5 cut(s) 352, 373, 400, 485, 1386
BssMI GATC 6 cut(s) 123, 178, 257, 902, 1457, 1623
BssT1I CCWWGG 2 cut(s) 400, 485
Bst2UI CCWGG 4 cut(s) 246, 375, 558, 1217
Bst4CI ACNGT 3 cut(s) 46, 964, 1043
BstBAI YACGTR 1 cut(s) 893
BstC8I GCNNGC 4 cut(s) 7, 622, 1087, 1160
BstDEI CTNAG 7 cut(s) 30, 433, 700, 727, 882, 1332, 1575
BstDSI CCRYGG 1 cut(s) 485
BstENI CCTNNNNNAGG 1 cut(s) 684
BstF5I GGATG 3 cut(s) 154, 1120, 1635
BstFNI CGCG 1 cut(s) 1162
BstKTI GATC 6 cut(s) 126, 181, 260, 905, 1460, 1626
BstMAI GTCTC 3 cut(s) 167, 1145, 1509
BstMBI GATC 6 cut(s) 123, 178, 257, 902, 1457, 1623
BstNI CCWGG 4 cut(s) 246, 375, 558, 1217
BstSCI CCNGG 7 cut(s) 244, 352, 373, 556, 1215, 1385, 1386
BstSFI CTRYAG 2 cut(s) 36, 516
BstUI CGCG 1 cut(s) 1162
BstV1I GCAGC 1 cut(s) 457
BstV2I GAAGAC 2 cut(s) 129, 1605
BstX2I RGATCY 2 cut(s) 257, 902
BstXI CCANNNNNNTGG 3 cut(s) 49, 407, 1302
BstYI RGATCY 2 cut(s) 257, 902
Bsu36I CCTNAGG 1 cut(s) 433
BsuI GTATCC 1 cut(s) 713
BsuRI GGCC 5 cut(s) 5, 399, 1085, 1215, 1220
BtgI CCRYGG 1 cut(s) 485
BtgZI GCGATG 1 cut(s) 266
BtsCI GGATG 3 cut(s) 154, 1120, 1635
BtsIMutI CAGTG 2 cut(s) 42, 1048
BveI ACCTGC 1 cut(s) 1540
Cac8I GCNNGC 4 cut(s) 7, 622, 1087, 1160
CaiI CAGNNNCTG 1 cut(s) 62
Cfr10I RCCGGY 1 cut(s) 5
Cfr13I GGNCC 3 cut(s) 554, 896, 1083
Cfr9I CCCGGG 1 cut(s) 1386
CsiI ACCWGGT 1 cut(s) 556
Csp6I GTAC 1 cut(s) 564
CspCI CAANNNNNGTGG 2 cut(s) 1264, 1299
CviAII CATG 4 cut(s) 133, 163, 200, 486
CviQI GTAC 1 cut(s) 564
DdeI CTNAG 7 cut(s) 30, 433, 700, 727, 882, 1332, 1575
DpnI GATC 6 cut(s) 125, 180, 259, 904, 1459, 1625
DpnII GATC 6 cut(s) 123, 178, 257, 902, 1457, 1623
DraI TTTAAA 1 cut(s) 1542
DraIII CACNNNGTG 1 cut(s) 1164
DriI GACNNNNNGTC 1 cut(s) 894
EaeI YGGCCR 3 cut(s) 3, 397, 1218
Eam1105I GACNNNNNGTC 1 cut(s) 894
Eco130I CCWWGG 2 cut(s) 400, 485
Eco147I AGGCCT 1 cut(s) 1215
Eco32I GATATC 1 cut(s) 511
Eco47I GGWCC 2 cut(s) 554, 896
Eco57I CTGAAG 4 cut(s) 486, 909, 1155, 1430
Eco72I CACGTG 1 cut(s) 893
Eco81I CCTNAGG 1 cut(s) 433
Eco88I CYCGRG 1 cut(s) 1386
EcoNI CCTNNNNNAGG 1 cut(s) 684
EcoRII CCWGG 4 cut(s) 244, 373, 556, 1215
EcoRV GATATC 1 cut(s) 511
EcoT14I CCWWGG 2 cut(s) 400, 485
ErhI CCWWGG 2 cut(s) 400, 485
FaeI CATG 4 cut(s) 136, 166, 203, 489
FaqI GGGAC 2 cut(s) 503, 1403
FatI CATG 4 cut(s) 132, 162, 199, 485
FauI CCCGC 2 cut(s) 81, 304
FauNDI CATATG 3 cut(s) 115, 367, 1581
FbaI TGATCA 1 cut(s) 178
Fnu4HI GCNGC 2 cut(s) 446, 1340
FokI GGATG 3 cut(s) 161, 1127, 1642
Fsp4HI GCNGC 2 cut(s) 446, 1340
FspBI CTAG 3 cut(s) 222, 576, 621
GluI GCNGC 2 cut(s) 446, 1340
HaeIII GGCC 5 cut(s) 5, 399, 1085, 1215, 1220
HapII CCGG 4 cut(s) 6, 69, 354, 1387
Hin1II CATG 4 cut(s) 136, 166, 203, 489
HincII GTYRAC 3 cut(s) 413, 888, 1273
HindII GTYRAC 3 cut(s) 413, 888, 1273
HinfI GANTC 7 cut(s) 59, 119, 185, 831, 872, 929, 1613
HpaII CCGG 4 cut(s) 6, 69, 354, 1387
HphI GGTGA 3 cut(s) 182, 518, 1201
Hpy166II GTNNAC 5 cut(s) 208, 413, 888, 1273, 1529
Hpy188I TCNGA 8 cut(s) 58, 325, 466, 694, 928, 999, 1095, 1639
Hpy188III TCNNGA 7 cut(s) 69, 182, 552, 1127, 1397, 1496, 1627
Hpy8I GTNNAC 5 cut(s) 208, 413, 888, 1273, 1529
HpyAV CCTTC 7 cut(s) 462, 654, 709, 729, 754, 933, 937
HpyCH4III ACNGT 3 cut(s) 46, 964, 1043
HpyCH4IV ACGT 1 cut(s) 892
HpyCH4V TGCA 4 cut(s) 319, 445, 1451, 1535
HpyF3I CTNAG 7 cut(s) 30, 433, 700, 727, 882, 1332, 1575
HpySE526I ACGT 1 cut(s) 892
Hsp92II CATG 4 cut(s) 136, 166, 203, 489
Kpn2I TCCGGA 1 cut(s) 68
KroI GCCGGC 1 cut(s) 5
KroNI GCCGGC 1 cut(s) 7
Ksp22I TGATCA 1 cut(s) 178
Kzo9I GATC 6 cut(s) 123, 178, 257, 902, 1457, 1623
LmnI GCTCC 1 cut(s) 1521
Lsp1109I GCAGC 1 cut(s) 457
LweI GCATC 3 cut(s) 62, 139, 1120
MabI ACCWGGT 1 cut(s) 556
MaeI CTAG 3 cut(s) 222, 576, 621
MaeII ACGT 1 cut(s) 892
MaeIII GTNAC 2 cut(s) 227, 1375
MalI GATC 6 cut(s) 125, 180, 259, 904, 1459, 1625
MboI GATC 6 cut(s) 123, 178, 257, 902, 1457, 1623
MboII GAAGA 7 cut(s) 28, 129, 132, 420, 1058, 1282, 1610
MflI RGATCY 2 cut(s) 257, 902
MlsI TGGCCA 1 cut(s) 399
MluCI AATT 4 cut(s) 232, 301, 626, 1480
MluNI TGGCCA 1 cut(s) 399
MlyI GAGTC 2 cut(s) 194, 923
MmeI TCCRAC 1 cut(s) 172
Mox20I TGGCCA 1 cut(s) 399
MroI TCCGGA 1 cut(s) 68
MroNI GCCGGC 1 cut(s) 5
MroXI GAANNNNTTC 1 cut(s) 1436
MscI TGGCCA 1 cut(s) 399
MseI TTAA 7 cut(s) 668, 777, 815, 1032, 1068, 1299, 1541
MslI CAYNNNNRTG 2 cut(s) 285, 1584
Msp20I TGGCCA 1 cut(s) 399
MspA1I CMGCKG 2 cut(s) 88, 731
MspI CCGG 4 cut(s) 6, 69, 354, 1387
MspR9I CCNGG 7 cut(s) 246, 354, 375, 558, 1217, 1387, 1388
MvaI CCWGG 4 cut(s) 246, 375, 558, 1217
MvnI CGCG 1 cut(s) 1162
NaeI GCCGGC 1 cut(s) 7
NciI CCSGG 3 cut(s) 354, 1387, 1388
NcoI CCATGG 1 cut(s) 485
NdeI CATATG 3 cut(s) 115, 367, 1581
NdeII GATC 6 cut(s) 123, 178, 257, 902, 1457, 1623
NgoMIV GCCGGC 1 cut(s) 5
NheI GCTAGC 1 cut(s) 620
NlaIII CATG 4 cut(s) 136, 166, 203, 489
NlaIV GGNNCC 2 cut(s) 259, 678
NmeAIII GCCGAG 1 cut(s) 1246
PceI AGGCCT 1 cut(s) 1215
PdiI GCCGGC 1 cut(s) 7
PdmI GAANNNNTTC 1 cut(s) 1436
PfeI GAWTC 5 cut(s) 59, 119, 831, 872, 1613
PfoI TCCNGGA 1 cut(s) 244
PkrI GCNGC 2 cut(s) 447, 1341
PleI GAGTC 2 cut(s) 193, 923
PmaCI CACGTG 1 cut(s) 893
PmlI CACGTG 1 cut(s) 893
PpsI GAGTC 2 cut(s) 193, 923
Ppu21I YACGTR 1 cut(s) 893
Psp6I CCWGG 4 cut(s) 244, 373, 556, 1215
PspCI CACGTG 1 cut(s) 893
PspGI CCWGG 4 cut(s) 244, 373, 556, 1215
PspN4I GGNNCC 2 cut(s) 259, 678
PspPI GGNCC 3 cut(s) 554, 896, 1083
PstNI CAGNNNCTG 1 cut(s) 62
PsuI RGATCY 2 cut(s) 257, 902
PvuII CAGCTG 1 cut(s) 731
RsaI GTAC 1 cut(s) 565
RsaNI GTAC 1 cut(s) 564
RseI CAYNNNNRTG 2 cut(s) 285, 1584
SaqAI TTAA 7 cut(s) 668, 777, 815, 1032, 1068, 1299, 1541
SatI GCNGC 2 cut(s) 446, 1340
Sau3AI GATC 6 cut(s) 123, 178, 257, 902, 1457, 1623
Sau96I GGNCC 3 cut(s) 554, 896, 1083
SchI GAGTC 2 cut(s) 194, 923
ScrFI CCNGG 7 cut(s) 246, 354, 375, 558, 1217, 1387, 1388
SexAI ACCWGGT 1 cut(s) 556
SfaNI GCATC 3 cut(s) 62, 139, 1120
SfcI CTRYAG 2 cut(s) 36, 516
SinI GGWCC 2 cut(s) 554, 896
SmaI CCCGGG 1 cut(s) 1388
SmiMI CAYNNNNRTG 2 cut(s) 285, 1584
SmlI CTYRAG 1 cut(s) 1395
SmoI CTYRAG 1 cut(s) 1395
Sse9I AATT 4 cut(s) 232, 301, 626, 1480
SseBI AGGCCT 1 cut(s) 1215
SsiI CCGC 4 cut(s) 88, 311, 1162, 1339
SspMI CTAG 3 cut(s) 222, 576, 621
StuI AGGCCT 1 cut(s) 1215
StyD4I CCNGG 7 cut(s) 244, 352, 373, 556, 1215, 1385, 1386
StyI CCWWGG 2 cut(s) 400, 485
TaaI ACNGT 3 cut(s) 46, 964, 1043
TaiI ACGT 1 cut(s) 895
TaqI TCGA 4 cut(s) 122, 143, 1145, 1495
TaqII GACCGA 1 cut(s) 1656
TasI AATT 4 cut(s) 232, 301, 626, 1480
TauI GCSGC 1 cut(s) 1342
TfiI GAWTC 5 cut(s) 59, 119, 831, 872, 1613
Tru1I TTAA 7 cut(s) 668, 777, 815, 1032, 1068, 1299, 1541
Tru9I TTAA 7 cut(s) 668, 777, 815, 1032, 1068, 1299, 1541
TscAI CASTG 2 cut(s) 49, 1048
TseI GCWGC 1 cut(s) 445
TspDTI ATGAA 3 cut(s) 179, 1023, 1503
TspMI CCCGGG 1 cut(s) 1386
TspRI CASTG 2 cut(s) 49, 1048
VpaK11BI GGWCC 2 cut(s) 554, 896
XagI CCTNNNNNAGG 1 cut(s) 684
XapI RAATTY 1 cut(s) 626
XmaI CCCGGG 1 cut(s) 1386
XmnI GAANNNNTTC 1 cut(s) 1436
XspI CTAG 3 cut(s) 222, 576, 621
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.