pycom16g01870

mediator of RNA polymerase II transcription subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0009426
Physical Location & Seq
Reverse (-)
1145764 .. 1149173
3410 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2187 bp
ATGTTAGAGCAGATATTAGCCACTAGTAATCACACATGGTCAGAGAAAACACTACGCTATTTCCCTTCTCTTCTTCGTGATTTTTCGATCCAACGGATTGATAAAAGGGGCCTGGCAATTCAAGAATGGCAGCAGGCAGAAACCACTGTAATTAACCAATGCACCCAGCTTTTGTCACCTTCCGCTGATCCTTCATATGTAATGACCTATATCAGTCATAGTTTTCCTCAACACCGACAATATCTATGTGCTGGTGCATGGATTCTGATGCAAGGGCACCCAGAAAACATCAACAGCCTAAACTTAGCACGCGTCTTGAGAGAGTTTTCTCCTGAGGAAGTATCATCTAATATTTATACAATGGTGGATGTTCTGCTTCATCATATTCAGTTGGAAATGCAGCACGGCCATTCTTTGCAGGACCTTTTATTGAAAGTATGTGCAAACCTAGCATTTTATATTTGGACCCATGAGTTGCTCCCTTTGGATATTTTGCTCTTGGCACTTATTGATCGTGACGATGACCCCCATGCTCTGCATATTGTGATTAGTCTACTTGATAGGCAAGAACTTCAACAAAGGGTGAAACAACATTGTTCGAATCGAGGTCCCCCTGAACACTGGCTTGTCTCTGCACCCTTTAAGCGAGTTGAATTGCAAAAGGCCCTCGGGAATCATCTATGTTGGAAGGACAGGTATCCCACCTTCTTTGATGATATTGCAGGGCGTTTGATCCCCGTCATTCCATTGATAATTTATAGACTAATAGAAAATGATGCCATAGATTCAGCTGATAGAATTCTGCTCATGTATTCTCCATTTCTTAAATACCACCCTTTAAGATTTACTTTTGTTCGTGACATACTTGCATATTTTTATGGCCATCTTCCCGGAAAGCTTATTGTCCGAATATTGAATGTACTCGAAGTCAACAAGATCCCATTTTCTGAGTCATTCCCGGTGCATATGAATAGTGCCATGTGCCCACCTGCTGATTACTTTGCAAGTCTCTTACTGGGACTAGTGAATAATGTCATTCCCCCATTGCATAACAACTCAAAATCTGGAACGAGTGATGCTTTAAACAATTCAATGCGTGCCCCACCTAACAAGACTCCACCACCGTCTCAGTCTGGGCAAGCAAATGTTTTTGACGGCCAAAAAGCATTCTACCAAATTCAGGATCCAGGCACATATACTCAGCTGGTTCTTGAAACAGCAGTAATTGAATTGCTCTCTCTTCCTGTATCTGCATCCCAGATTGTATCATCACTTGTTCAGATTGTCGTCAATATACAACCAACATTAATTCAATCCAGCAATGGCCTGCACGGAGCTCCCAGTGGTGTTGGGCAAGGCTCCATTTTACCAACATCCCCTTCGGGCGGAAGCACAGATTCCTTGGGCACGAGCAGGTCACCTGCTTCAGTATCAGGAATAAATGCATCTAACTTTGTTTCTCGAAGTGGTTATACATGCCAACAATTGTCTTGCTTATTGATCCAAGCCTGTGGTCTACTACTGGCTCAGCTGCCTCCTGATTTTCATGTGCAACTCTATCTGGAGGCATCACGTATAATAAAAGAAACGTGGTGGCTCACTGATGGCAAAAGGTCAATGGGGGAACTGGACTCTGCTGTCGGTTATGCTTTGTTGGATCCAACATGGGCTGCTCAAGACAATACCTCAACCGCCATTGGTAATATTGTCGCGCTGCTTCATTCTTTTTTCAGTAACCTCCCACAGGAATGGCTAGAAGGGACCCATCTTATCATCAAACATCTCAGGCCAGTGACTTCAGTTGCAATGTTGAGAATAGCATTCCGGATCATGGGTCCATTGCTCCCTAAACTAGCCAATGCGCACGCCCTCTTCAGCAAGACCTTGTCATTGATTTTAAGTATGATGGTGGATGTCTTTGGGAAGAACGTACAGCCACCGACTCCTGTCGAGCCATTAGAAATAGCAGATCTTATTGACTTCTTCCATCACGTCGTCCATTATGAAGGGCAGGGAGGTCCCGTGCAGGCTAACAGCAAGCCCAGACCAGAGGTCTTAGCTCTCTGTGGAAGAGCTGCGGAAAGTCTGCGTCCGGACATACAACACCTTCTTTCCCACTTAAAACCCGACACGAATTCTTCCATTTATGCTGCTACGCATCCCAAGTTGAACCAGAATTCATCGTAA

Protein Analysis

729

Amino Acids

80.97

Weight (kDa)

6.37

Isoelectric Point (pI)

45.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Med23 PF11573 2 - 296 1.3e-20 Mediator complex subunit 23
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 997, 1429
AasI GACNNNNNNGTC 1 cut(s) 1637
Acc16I TGCGCA 1 cut(s) 1863
Acc36I ACCTGC 3 cut(s) 997, 1404, 1429
AccB1I GGYRCC 1 cut(s) 276
AccI GTMKAC 2 cut(s) 553, 1516
AccII CGCG 2 cut(s) 312, 1712
AccIII TCCGGA 2 cut(s) 1824, 2092
AciI CCGC 4 cut(s) 183, 1386, 1692, 2078
AcoI YGGCCR 3 cut(s) 406, 880, 1156
AcsI RAATTY 4 cut(s) 798, 1176, 2134, 2176
AcuI CTGAAG 3 cut(s) 1410, 1782, 1858
AfaI GTAC 2 cut(s) 921, 1932
AfiI CCNNNNNNNGG 4 cut(s) 1180, 1385, 1744, 1831
AflIII ACRYGT 1 cut(s) 310
AhdI GACNNNNNGTC 1 cut(s) 2051
AhlI ACTAGT 2 cut(s) 23, 1021
AjiI CACGTC 1 cut(s) 1993
AjnI CCWGG 2 cut(s) 111, 1186
AjuI GAANNNNNNNTTGG 2 cut(s) 1363, 1395
AluBI AGCT 8 cut(s) 169, 791, 898, 1204, 1337, 1531, 2060, 2075
AluI AGCT 8 cut(s) 169, 791, 898, 1204, 1337, 1531, 2060, 2075
Alw21I GWGCWC 1 cut(s) 1339
Alw26I GTCTC 3 cut(s) 634, 1013, 1131
Ama87I CYCGRG 1 cut(s) 668
Aor13HI TCCGGA 2 cut(s) 1824, 2092
AoxI GGCC 7 cut(s) 109, 406, 663, 880, 1156, 1324, 1787
ApeKI GCWGC 7 cut(s) 130, 400, 1531, 1670, 1714, 2075, 2150
ApoI RAATTY 4 cut(s) 798, 1176, 2134, 2176
AseI ATTAAT 1 cut(s) 1307
AspLEI GCGC 2 cut(s) 1714, 1864
AspS9I GGNCC 8 cut(s) 109, 421, 465, 608, 664, 1761, 1835, 2018
AsuC2I CCSGG 2 cut(s) 891, 959
AsuHPI GGTGA 3 cut(s) 168, 595, 1410
AsuII TTCGAA 1 cut(s) 599
AvaI CYCGRG 1 cut(s) 668
AvaII GGWCC 6 cut(s) 421, 465, 608, 1761, 1835, 2018
AxyI CCTNAGG 1 cut(s) 333
BaeGI GKGCMC 4 cut(s) 279, 986, 1102, 1409
BalI TGGCCA 1 cut(s) 882
BamHI GGATCC 2 cut(s) 1183, 1657
BanI GGYRCC 1 cut(s) 276
BanII GRGCYC 1 cut(s) 1339
BarI GAAGNNNNNNTAC 4 cut(s) 689, 721, 1456, 1488
BauI CACGAG 1 cut(s) 1408
Bbv12I GWGCWC 1 cut(s) 1339
BbvI GCAGC 7 cut(s) 142, 412, 1518, 1657, 1701, 2062, 2137
BccI CCATC 5 cut(s) 891, 1598, 1773, 1900, 1995
BceAI ACGGC 2 cut(s) 421, 1171
BciT130I CCWGG 2 cut(s) 113, 1188
BciVI GTATCC 1 cut(s) 708
BcnI CCSGG 2 cut(s) 891, 959
BcoDI GTCTC 3 cut(s) 634, 1013, 1131
BcuI ACTAGT 2 cut(s) 23, 1021
BfaI CTAG 5 cut(s) 24, 449, 1022, 1754, 1853
BfuAI ACCTGC 3 cut(s) 997, 1404, 1429
BfuI GTATCC 1 cut(s) 708
BglII AGATCT 1 cut(s) 1969
BisI GCNGC 7 cut(s) 131, 401, 1532, 1671, 1715, 2076, 2151
BlpI GCTNAGC 1 cut(s) 1527
BlsI GCNGC 7 cut(s) 132, 402, 1533, 1672, 1716, 2077, 2152
Bme1390I CCNGG 4 cut(s) 113, 891, 959, 1188
Bme18I GGWCC 6 cut(s) 421, 465, 608, 1761, 1835, 2018
BmeRI GACNNNNNGTC 1 cut(s) 2051
BmeT110I CYCGRG 1 cut(s) 668
BmgBI CACGTC 1 cut(s) 1993
BmgT120I GGNCC 8 cut(s) 109, 421, 465, 608, 664, 1761, 1835, 2018
BmrFI CCNGG 4 cut(s) 113, 891, 959, 1188
BmrI ACTGGG 2 cut(s) 1025, 1335
BmsI GCATC 7 cut(s) 258, 766, 1066, 1262, 1454, 1577, 2167
BmuI ACTGGG 2 cut(s) 1025, 1335
BoxI GACNNNNGTC 1 cut(s) 1946
BpmI CTGGAG 1 cut(s) 1583
Bpu1102I GCTNAGC 1 cut(s) 1527
Bpu14I TTCGAA 1 cut(s) 599
BpuEI CTTGAG 2 cut(s) 337, 1659
BpuMI CCSGG 2 cut(s) 891, 959
BsaAI YACGTR 1 cut(s) 1574
BsaJI CCNNGG 2 cut(s) 667, 1401
BsaWI WCCGGW 2 cut(s) 1824, 2092
BsaXI ACNNNNNCTCC 2 cut(s) 2007, 2037
Bsc4I CCNNNNNNNGG 4 cut(s) 1180, 1385, 1744, 1831
Bse1I ACTGG 6 cut(s) 626, 1020, 1341, 1527, 1632, 1790
Bse21I CCTNAGG 1 cut(s) 333
Bse3DI GCAATG 4 cut(s) 1043, 1327, 1812, 1838
BseAI TCCGGA 2 cut(s) 1824, 2092
BseBI CCWGG 2 cut(s) 113, 1188
BseDI CCNNGG 2 cut(s) 667, 1401
BseGI GGATG 5 cut(s) 373, 1253, 1373, 1918, 2158
BseLI CCNNNNNNNGG 4 cut(s) 1180, 1385, 1744, 1831
BseMI GCAATG 4 cut(s) 1043, 1327, 1812, 1838
BseMII CTCAG 6 cut(s) 324, 939, 1142, 1214, 1541, 1798
BseNI ACTGG 6 cut(s) 626, 1020, 1341, 1527, 1632, 1790
BseSI GKGCMC 4 cut(s) 279, 986, 1102, 1409
BseXI GCAGC 7 cut(s) 142, 412, 1518, 1657, 1701, 2062, 2137
BseYI CCCAGC 1 cut(s) 165
BsgI GTGCAG 3 cut(s) 618, 1313, 2045
Bsh1236I CGCG 2 cut(s) 312, 1712
BshFI GGCC 7 cut(s) 111, 408, 665, 882, 1158, 1326, 1789
BshNI GGYRCC 1 cut(s) 276
BsiHKAI GWGCWC 1 cut(s) 1339
BsiHKCI CYCGRG 1 cut(s) 668
BsiSI CCGG 4 cut(s) 891, 959, 1825, 2093
BslFI GGGAC 4 cut(s) 594, 1032, 1774, 2004
BslI CCNNNNNNNGG 4 cut(s) 1180, 1385, 1744, 1831
BsmAI GTCTC 3 cut(s) 634, 1013, 1131
BsmBI CGTCTC 1 cut(s) 1131
BsmFI GGGAC 4 cut(s) 594, 1032, 1774, 2004
BsmI GAATGC 2 cut(s) 1166, 1820
BsnI GGCC 7 cut(s) 111, 408, 665, 882, 1158, 1326, 1789
BsoBI CYCGRG 1 cut(s) 668
Bsp119I TTCGAA 1 cut(s) 599
Bsp1286I GDGCHC 5 cut(s) 279, 986, 1102, 1339, 1409
Bsp13I TCCGGA 2 cut(s) 1824, 2092
Bsp1720I GCTNAGC 1 cut(s) 1527
BspACI CCGC 4 cut(s) 183, 1386, 1692, 2078
BspANI GGCC 7 cut(s) 111, 408, 665, 882, 1158, 1326, 1789
BspCNI CTCAG 6 cut(s) 325, 940, 1141, 1213, 1540, 1797
BspEI TCCGGA 2 cut(s) 1824, 2092
BspFNI CGCG 2 cut(s) 312, 1712
BspMI ACCTGC 3 cut(s) 997, 1404, 1429
BspQI GCTCTTC 1 cut(s) 2065
BspT104I TTCGAA 1 cut(s) 599
BspT107I GGYRCC 1 cut(s) 276
BsrDI GCAATG 4 cut(s) 1043, 1327, 1812, 1838
BsrI ACTGG 6 cut(s) 626, 1020, 1341, 1527, 1632, 1790
BssECI CCNNGG 2 cut(s) 667, 1401
BssSI CACGAG 1 cut(s) 1408
BssT1I CCWWGG 1 cut(s) 1401
Bst2BI CACGAG 1 cut(s) 1408
Bst2UI CCWGG 2 cut(s) 113, 1188
Bst4CI ACNGT 2 cut(s) 148, 1125
Bst6I CTCTTC 4 cut(s) 75, 1245, 1877, 2065
BstBAI YACGTR 1 cut(s) 1574
BstBI TTCGAA 1 cut(s) 599
BstC8I GCNNGC 8 cut(s) 135, 310, 1098, 1140, 1328, 1866, 2028, 2039
BstDEI CTNAG 8 cut(s) 304, 333, 948, 1128, 1200, 1527, 1784, 2056
BstEII GGTNACC 1 cut(s) 1416
BstENI CCTNNNNNAGG 1 cut(s) 1742
BstF5I GGATG 5 cut(s) 373, 1253, 1373, 1918, 2158
BstFNI CGCG 2 cut(s) 312, 1712
BstHHI GCGC 2 cut(s) 1714, 1864
BstMAI GTCTC 3 cut(s) 634, 1013, 1131
BstMWI GCNNNNNNNGC 1 cut(s) 449
BstNI CCWGG 2 cut(s) 113, 1188
BstNSI RCATGY 1 cut(s) 1479
BstPAI GACNNNNGTC 1 cut(s) 1946
BstPI GGTNACC 1 cut(s) 1416
BstSCI CCNGG 4 cut(s) 111, 889, 957, 1186
BstSLI GKGCMC 4 cut(s) 279, 986, 1102, 1409
BstUI CGCG 2 cut(s) 312, 1712
BstV1I GCAGC 7 cut(s) 142, 412, 1518, 1657, 1701, 2062, 2137
BstX2I RGATCY 4 cut(s) 936, 1183, 1657, 1969
BstXI CCANNNNNNTGG 2 cut(s) 1511, 1749
BstYI RGATCY 4 cut(s) 936, 1183, 1657, 1969
Bsu36I CCTNAGG 1 cut(s) 333
BsuI GTATCC 1 cut(s) 708
BsuRI GGCC 7 cut(s) 111, 408, 665, 882, 1158, 1326, 1789
BtrI CACGTC 1 cut(s) 1993
BtsCI GGATG 5 cut(s) 373, 1253, 1373, 1918, 2158
BtsIMutI CAGTG 5 cut(s) 144, 619, 1348, 1599, 1797
BveI ACCTGC 3 cut(s) 997, 1404, 1429
Cac8I GCNNGC 8 cut(s) 135, 310, 1098, 1140, 1328, 1866, 2028, 2039
CfoI GCGC 2 cut(s) 1714, 1864
Cfr13I GGNCC 8 cut(s) 109, 421, 465, 608, 664, 1761, 1835, 2018
CseI GACGC 2 cut(s) 301, 2078
Csp6I GTAC 2 cut(s) 920, 1931
CspCI CAANNNNNGTGG 2 cut(s) 691, 726
CviQI GTAC 2 cut(s) 920, 1931
DdeI CTNAG 8 cut(s) 304, 333, 948, 1128, 1200, 1527, 1784, 2056
DraI TTTAAA 1 cut(s) 1083
DrdI GACNNNNNNGTC 1 cut(s) 1637
DriI GACNNNNNGTC 1 cut(s) 2051
DseDI GACNNNNNNGTC 1 cut(s) 1637
EaeI YGGCCR 3 cut(s) 406, 880, 1156
Eam1104I CTCTTC 4 cut(s) 75, 1245, 1877, 2065
Eam1105I GACNNNNNGTC 1 cut(s) 2051
EarI CTCTTC 4 cut(s) 75, 1245, 1877, 2065
EciI GGCGGA 1 cut(s) 1401
Ecl136II GAGCTC 1 cut(s) 1337
Eco130I CCWWGG 1 cut(s) 1401
Eco24I GRGCYC 1 cut(s) 1339
Eco47I GGWCC 6 cut(s) 421, 465, 608, 1761, 1835, 2018
Eco53kI GAGCTC 1 cut(s) 1337
Eco57I CTGAAG 3 cut(s) 1410, 1782, 1858
Eco81I CCTNAGG 1 cut(s) 333
Eco88I CYCGRG 1 cut(s) 668
Eco91I GGTNACC 1 cut(s) 1416
EcoICRI GAGCTC 1 cut(s) 1337
EcoNI CCTNNNNNAGG 1 cut(s) 1742
EcoO109I RGGNCCY 6 cut(s) 109, 421, 608, 664, 1761, 2018
EcoO65I GGTNACC 1 cut(s) 1416
EcoRI GAATTC 3 cut(s) 798, 2134, 2176
EcoRII CCWGG 2 cut(s) 111, 1186
EcoT14I CCWWGG 1 cut(s) 1401
EcoT22I ATGCAT 1 cut(s) 1447
EcoT38I GRGCYC 1 cut(s) 1339
ErhI CCWWGG 1 cut(s) 1401
Esp3I CGTCTC 1 cut(s) 1131
FalI AAGNNNNNCTT 2 cut(s) 832, 864
FaqI GGGAC 4 cut(s) 594, 1032, 1774, 2004
FauNDI CATATG 2 cut(s) 196, 966
FblI GTMKAC 2 cut(s) 553, 1516
Fnu4HI GCNGC 7 cut(s) 131, 401, 1532, 1671, 1715, 2076, 2151
FokI GGATG 5 cut(s) 380, 1240, 1360, 1925, 2145
FriOI GRGCYC 1 cut(s) 1339
Fsp4HI GCNGC 7 cut(s) 131, 401, 1532, 1671, 1715, 2076, 2151
FspAI RTGCGCAY 1 cut(s) 1863
FspBI CTAG 5 cut(s) 24, 449, 1022, 1754, 1853
FspI TGCGCA 1 cut(s) 1863
GlaI GCGC 2 cut(s) 1713, 1863
GluI GCNGC 7 cut(s) 131, 401, 1532, 1671, 1715, 2076, 2151
GsaI CCCAGC 1 cut(s) 169
GsuI CTGGAG 1 cut(s) 1583
HaeIII GGCC 7 cut(s) 111, 408, 665, 882, 1158, 1326, 1789
HapII CCGG 4 cut(s) 891, 959, 1825, 2093
HgaI GACGC 2 cut(s) 301, 2078
HhaI GCGC 2 cut(s) 1714, 1864
Hin6I GCGC 2 cut(s) 1712, 1862
HinP1I GCGC 2 cut(s) 1712, 1862
HincII GTYRAC 1 cut(s) 931
HindII GTYRAC 1 cut(s) 931
HindIII AAGCTT 1 cut(s) 896
HinfI GANTC 9 cut(s) 262, 601, 673, 785, 950, 1114, 1397, 1631, 1942
HpaII CCGG 4 cut(s) 891, 959, 1825, 2093
HphI GGTGA 3 cut(s) 168, 595, 1410
Hpy166II GTNNAC 3 cut(s) 554, 931, 1517
Hpy188I TCNGA 5 cut(s) 43, 267, 908, 949, 1281
Hpy8I GTNNAC 3 cut(s) 554, 931, 1517
Hpy99I CGWCG 1 cut(s) 1997
HpyAV CCTTC 9 cut(s) 75, 189, 201, 682, 715, 1389, 1751, 2000, 2117
HpyCH4III ACNGT 2 cut(s) 148, 1125
HpyCH4IV ACGT 4 cut(s) 1573, 1589, 1929, 1992
HpyF10VI GCNNNNNNNGC 1 cut(s) 449
HpyF3I CTNAG 8 cut(s) 304, 333, 948, 1128, 1200, 1527, 1784, 2056
HpySE526I ACGT 4 cut(s) 1573, 1589, 1929, 1992
HspAI GCGC 2 cut(s) 1712, 1862
KflI GGGWCCC 1 cut(s) 1761
Kpn2I TCCGGA 2 cut(s) 1824, 2092
LguI GCTCTTC 1 cut(s) 2065
LmnI GCTCC 5 cut(s) 483, 1334, 1342, 1364, 1848
Lsp1109I GCAGC 7 cut(s) 142, 412, 1518, 1657, 1701, 2062, 2137
LweI GCATC 7 cut(s) 258, 766, 1066, 1262, 1454, 1577, 2167
MaeI CTAG 5 cut(s) 24, 449, 1022, 1754, 1853
MaeII ACGT 4 cut(s) 1573, 1589, 1929, 1992
MaeIII GTNAC 6 cut(s) 174, 515, 857, 1416, 1733, 1792
MboII GAAGA 9 cut(s) 62, 65, 878, 1232, 1864, 1936, 1975, 2082, 2130
MfeI CAATTG 1 cut(s) 1484
MflI RGATCY 4 cut(s) 936, 1183, 1657, 1969
MhlI GDGCHC 5 cut(s) 279, 986, 1102, 1339, 1409
MlsI TGGCCA 1 cut(s) 882
MluI ACGCGT 1 cut(s) 310
MluNI TGGCCA 1 cut(s) 882
MlyI GAGTC 4 cut(s) 959, 1108, 1625, 1936
MmeI TCCRAC 5 cut(s) 115, 372, 665, 1635, 1685
Mox20I TGGCCA 1 cut(s) 882
Mph1103I ATGCAT 1 cut(s) 1447
MroI TCCGGA 2 cut(s) 1824, 2092
MscI TGGCCA 1 cut(s) 882
MseI TTAA 8 cut(s) 153, 642, 825, 839, 1082, 1307, 1898, 2120
MslI CAYNNNNRTG 1 cut(s) 1747
Msp20I TGGCCA 1 cut(s) 882
MspA1I CMGCKG 4 cut(s) 185, 791, 1204, 1531
MspI CCGG 4 cut(s) 891, 959, 1825, 2093
MspR9I CCNGG 4 cut(s) 113, 891, 959, 1188
MunI CAATTG 1 cut(s) 1484
Mva1269I GAATGC 2 cut(s) 1166, 1820
MvaI CCWGG 2 cut(s) 113, 1188
MvnI CGCG 2 cut(s) 312, 1712
MwoI GCNNNNNNNGC 1 cut(s) 449
NciI CCSGG 2 cut(s) 891, 959
NdeI CATATG 2 cut(s) 196, 966
NmuCI GTSAC 5 cut(s) 174, 515, 857, 1416, 1792
NsbI TGCGCA 1 cut(s) 1863
NsiI ATGCAT 1 cut(s) 1447
NspI RCATGY 1 cut(s) 1479
NspV TTCGAA 1 cut(s) 599
PaqCI CACCTGC 2 cut(s) 997, 1429
PciSI GCTCTTC 1 cut(s) 2065
PctI GAATGC 2 cut(s) 1166, 1820
PfeI GAWTC 5 cut(s) 262, 601, 673, 785, 1397
PflFI GACNNNGTC 1 cut(s) 1885
PfoI TCCNGGA 1 cut(s) 889
PkrI GCNGC 7 cut(s) 132, 402, 1533, 1672, 1716, 2077, 2152
PleI GAGTC 4 cut(s) 958, 1108, 1625, 1936
PpsI GAGTC 4 cut(s) 958, 1108, 1625, 1936
Ppu21I YACGTR 1 cut(s) 1574
PpuMI RGGWCCY 4 cut(s) 421, 608, 1761, 2018
PshAI GACNNNNGTC 1 cut(s) 1946
PshBI ATTAAT 1 cut(s) 1307
Psp124BI GAGCTC 1 cut(s) 1339
Psp5II RGGWCCY 4 cut(s) 421, 608, 1761, 2018
Psp6I CCWGG 2 cut(s) 111, 1186
PspEI GGTNACC 1 cut(s) 1416
PspFI CCCAGC 1 cut(s) 165
PspGI CCWGG 2 cut(s) 111, 1186
PspPI GGNCC 8 cut(s) 109, 421, 465, 608, 664, 1761, 1835, 2018
PspPPI RGGWCCY 4 cut(s) 421, 608, 1761, 2018
PsuI RGATCY 4 cut(s) 936, 1183, 1657, 1969
PsyI GACNNNGTC 1 cut(s) 1885
PvuII CAGCTG 3 cut(s) 791, 1204, 1531
RsaI GTAC 2 cut(s) 921, 1932
RsaNI GTAC 2 cut(s) 920, 1931
RseI CAYNNNNRTG 1 cut(s) 1747
SacI GAGCTC 1 cut(s) 1339
SapI GCTCTTC 1 cut(s) 2065
SaqAI TTAA 8 cut(s) 153, 642, 825, 839, 1082, 1307, 1898, 2120
SatI GCNGC 7 cut(s) 131, 401, 1532, 1671, 1715, 2076, 2151
Sau96I GGNCC 8 cut(s) 109, 421, 465, 608, 664, 1761, 1835, 2018
SchI GAGTC 4 cut(s) 959, 1108, 1625, 1936
ScrFI CCNGG 4 cut(s) 113, 891, 959, 1188
SduI GDGCHC 5 cut(s) 279, 986, 1102, 1339, 1409
SfaNI GCATC 7 cut(s) 258, 766, 1066, 1262, 1454, 1577, 2167
SfuI TTCGAA 1 cut(s) 599
SinI GGWCC 6 cut(s) 421, 465, 608, 1761, 1835, 2018
SmiMI CAYNNNNRTG 1 cut(s) 1747
SmlI CTYRAG 2 cut(s) 316, 1674
SmoI CTYRAG 2 cut(s) 316, 1674
SpeI ACTAGT 2 cut(s) 23, 1021
SsiI CCGC 4 cut(s) 183, 1386, 1692, 2078
SspI AATATT 3 cut(s) 352, 912, 1705
SspMI CTAG 5 cut(s) 24, 449, 1022, 1754, 1853
SstI GAGCTC 1 cut(s) 1339
StyD4I CCNGG 4 cut(s) 111, 889, 957, 1186
StyI CCWWGG 1 cut(s) 1401
TaaI ACNGT 2 cut(s) 148, 1125
TaiI ACGT 4 cut(s) 1576, 1592, 1932, 1995
TaqI TCGA 6 cut(s) 86, 599, 604, 924, 1462, 1950
TatI WGTACW 1 cut(s) 919
TfiI GAWTC 5 cut(s) 262, 601, 673, 785, 1397
Tru1I TTAA 8 cut(s) 153, 642, 825, 839, 1082, 1307, 1898, 2120
Tru9I TTAA 8 cut(s) 153, 642, 825, 839, 1082, 1307, 1898, 2120
TscAI CASTG 5 cut(s) 151, 626, 1348, 1606, 1797
TseFI GTSAC 5 cut(s) 174, 515, 857, 1416, 1792
TseI GCWGC 7 cut(s) 130, 400, 1531, 1670, 1714, 2075, 2150
Tsp45I GTSAC 5 cut(s) 174, 515, 857, 1416, 1792
TspDTI ATGAA 7 cut(s) 183, 368, 983, 1535, 1709, 2019, 2169
TspGWI ACGGA 2 cut(s) 109, 1347
TspRI CASTG 5 cut(s) 151, 626, 1348, 1606, 1797
Tth111I GACNNNGTC 1 cut(s) 1885
VpaK11BI GGWCC 6 cut(s) 421, 465, 608, 1761, 1835, 2018
VspI ATTAAT 1 cut(s) 1307
XagI CCTNNNNNAGG 1 cut(s) 1742
XapI RAATTY 4 cut(s) 798, 1176, 2134, 2176
XceI RCATGY 1 cut(s) 1479
XmiI GTMKAC 2 cut(s) 553, 1516
XspI CTAG 5 cut(s) 24, 449, 1022, 1754, 1853
Zsp2I ATGCAT 1 cut(s) 1447
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.