pycom16g02020

quinone binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0011990
Physical Location & Seq
Forward (+)
1270076 .. 1271393
1318 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 567 bp
ATGAGCTGCTCATTCAGCTTCCAAATCCCCCAGGCGCTGCCTTCTCTCGGCCGTTCCCGGCGTTCTTCTTCTCTCCCAATCGCCTCTCAACACAGACAGCCCAACAATGTCGATCCTCAAAAGACAGTCGGAAGCATCCGTCAAAACCCAATTGACTGTTTCCACCTGAAGAAATCGAACTTGGCAATTCAAGTTGGAGCAGTTTTGGCCACCCAGATTGAGCAGCCTGCATTTGCCGTCACCGGAATCAACTACGAGGAAGATTTGACATGGGTTTTAATACAGTCCGGAGTTGTTGCCTTCTGGTACTTCCTTATCATGCCACCAATCATCATGAACTGGCTTCGGATTAGGTGGTACAAGAGAAATCTTCTAGAGATGTACTTTCAGTTCATGTTTGTCTTCTTGTTCTTTCCTGGAGTAATACTTTGGGCACCATTTCTGAACTTCAGGAAGTTCCCACGAGATCGATCTATGAAGTACCCTTGGTCGAAGCCAGACGATCCTGACCAAATAAAAGCTGGGTTTCTAAAGTACCCATTTGCAGAACCTGAAGATTACTCGTAG

Protein Analysis

189

Amino Acids

21.99

Weight (kDa)

9.45

Isoelectric Point (pI)

57.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NdhL PF10716 84 - 156 2.1e-31 NADH dehydrogenase transmembrane subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011990)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G70760
fragaria_vesca FvH4_4g34090
malus_domestica MD13G1021900.v1.1 MD16G1022900.v1.1
prunus_persica Prupe.1G330000_v2.0.a1 Prupe.1G330000_v2.0.a1
pyrus_communis pycom16g02020
rosa_chinensis RchiOBHm_Chr4g0443591
rosa_laevigata RLG00000005912
rosa_multiflora Rmu_sc0011070.1_g000002 Rmu_sc0026347.1_g000005
rosa_roxburghii Rroxscaffold_5G00384140
rosa_samantha Rh4AG403300 Rh4AG405200 Rh4BG416200 Rh4CG430700 Rh4DG410600 Rh4DG411800
rosa_wichuraiana Rw4G034790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 433
AccIII TCCGGA 1 cut(s) 287
AclWI GGATC 2 cut(s) 107, 497
AcoI YGGCCR 2 cut(s) 49, 207
AcuI CTGAAG 2 cut(s) 188, 433
AfaI GTAC 5 cut(s) 308, 359, 383, 482, 536
AfiI CCNNNNNNNGG 1 cut(s) 47
AgsI TTSAA 1 cut(s) 191
AjnI CCWGG 2 cut(s) 30, 415
AjuI GAANNNNNNNTTGG 2 cut(s) 164, 196
AluBI AGCT 3 cut(s) 6, 18, 521
AluI AGCT 3 cut(s) 6, 18, 521
AlwI GGATC 2 cut(s) 107, 497
AlwNI CAGNNNCTG 2 cut(s) 37, 551
Aor13HI TCCGGA 1 cut(s) 287
AoxI GGCC 2 cut(s) 49, 207
ApeKI GCWGC 3 cut(s) 6, 37, 223
AspLEI GCGC 1 cut(s) 37
AsuC2I CCSGG 1 cut(s) 58
AsuHPI GGTGA 1 cut(s) 232
BaeGI GKGCMC 1 cut(s) 436
BalI TGGCCA 1 cut(s) 209
BanI GGYRCC 1 cut(s) 433
BauI CACGAG 1 cut(s) 462
BbsI GAAGAC 1 cut(s) 394
BbvI GCAGC 2 cut(s) 24, 235
BceAI ACGGC 2 cut(s) 36, 221
BciT130I CCWGG 2 cut(s) 32, 417
BcnI CCSGG 1 cut(s) 58
BfaI CTAG 1 cut(s) 374
BfoI RGCGCY 1 cut(s) 38
BisI GCNGC 3 cut(s) 7, 38, 224
BlsI GCNGC 3 cut(s) 8, 39, 225
Bme1390I CCNGG 3 cut(s) 32, 58, 417
BmiI GGNNCC 1 cut(s) 435
BmrFI CCNGG 3 cut(s) 32, 58, 417
BmsI GCATC 1 cut(s) 144
BpiI GAAGAC 1 cut(s) 394
BpmI CTGGAG 1 cut(s) 438
BpuMI CCSGG 1 cut(s) 58
Bsa29I ATCGAT 1 cut(s) 469
BsaJI CCNNGG 2 cut(s) 30, 485
BsaWI WCCGGW 2 cut(s) 242, 287
Bsc4I CCNNNNNNNGG 1 cut(s) 47
Bse1I ACTGG 1 cut(s) 344
BseAI TCCGGA 1 cut(s) 287
BseBI CCWGG 2 cut(s) 32, 417
BseCI ATCGAT 1 cut(s) 469
BseDI CCNNGG 2 cut(s) 30, 485
BseGI GGATG 1 cut(s) 135
BseLI CCNNNNNNNGG 1 cut(s) 47
BseNI ACTGG 1 cut(s) 344
BseSI GKGCMC 1 cut(s) 436
BseX3I CGGCCG 1 cut(s) 49
BseXI GCAGC 2 cut(s) 24, 235
BseYI CCCAGC 1 cut(s) 521
Bsh1285I CGRYCG 1 cut(s) 52
BshFI GGCC 2 cut(s) 51, 209
BshNI GGYRCC 1 cut(s) 433
BshVI ATCGAT 1 cut(s) 469
BsiEI CGRYCG 1 cut(s) 52
BsiSI CCGG 3 cut(s) 58, 243, 288
BslI CCNNNNNNNGG 1 cut(s) 47
BsnI GGCC 2 cut(s) 51, 209
Bsp1286I GDGCHC 1 cut(s) 436
Bsp13I TCCGGA 1 cut(s) 287
Bsp143I GATC 4 cut(s) 112, 466, 470, 502
BspANI GGCC 2 cut(s) 51, 209
BspDI ATCGAT 1 cut(s) 469
BspEI TCCGGA 1 cut(s) 287
BspHI TCATGA 1 cut(s) 333
BspLI GGNNCC 1 cut(s) 435
BspPI GGATC 2 cut(s) 107, 497
BspT107I GGYRCC 1 cut(s) 433
BsrI ACTGG 1 cut(s) 344
BssECI CCNNGG 2 cut(s) 30, 485
BssMI GATC 4 cut(s) 112, 466, 470, 502
BssSI CACGAG 1 cut(s) 462
BssT1I CCWWGG 1 cut(s) 485
Bst2BI CACGAG 1 cut(s) 462
Bst2UI CCWGG 2 cut(s) 32, 417
Bst4CI ACNGT 3 cut(s) 127, 158, 285
BstC8I GCNNGC 1 cut(s) 228
BstF5I GGATG 1 cut(s) 135
BstH2I RGCGCY 1 cut(s) 38
BstHHI GCGC 1 cut(s) 37
BstKTI GATC 4 cut(s) 115, 469, 473, 505
BstMBI GATC 4 cut(s) 112, 466, 470, 502
BstMCI CGRYCG 1 cut(s) 52
BstMWI GCNNNNNNNGC 2 cut(s) 15, 206
BstNI CCWGG 2 cut(s) 32, 417
BstSCI CCNGG 3 cut(s) 30, 56, 415
BstSLI GKGCMC 1 cut(s) 436
BstV1I GCAGC 2 cut(s) 24, 235
BstV2I GAAGAC 1 cut(s) 394
BstZI CGGCCG 1 cut(s) 49
Bsu15I ATCGAT 1 cut(s) 469
BsuRI GGCC 2 cut(s) 51, 209
BsuTUI ATCGAT 1 cut(s) 469
BtsCI GGATG 1 cut(s) 135
Cac8I GCNNGC 1 cut(s) 228
CaiI CAGNNNCTG 2 cut(s) 37, 551
CciI TCATGA 1 cut(s) 333
CfoI GCGC 1 cut(s) 37
ClaI ATCGAT 1 cut(s) 469
Csp6I GTAC 5 cut(s) 307, 358, 382, 481, 535
CspCI CAANNNNNGTGG 2 cut(s) 199, 234
CviAII CATG 4 cut(s) 270, 319, 334, 394
CviJI RGCY 9 cut(s) 6, 18, 51, 100, 209, 226, 343, 496, 521
CviKI_1 RGCY 9 cut(s) 6, 18, 51, 100, 209, 226, 343, 496, 521
CviQI GTAC 5 cut(s) 307, 358, 382, 481, 535
DpnI GATC 4 cut(s) 114, 468, 472, 504
DpnII GATC 4 cut(s) 112, 466, 470, 502
EaeI YGGCCR 2 cut(s) 49, 207
EagI CGGCCG 1 cut(s) 49
EclXI CGGCCG 1 cut(s) 49
Eco130I CCWWGG 1 cut(s) 485
Eco52I CGGCCG 1 cut(s) 49
Eco57I CTGAAG 2 cut(s) 188, 433
EcoRII CCWGG 2 cut(s) 30, 415
EcoT14I CCWWGG 1 cut(s) 485
ErhI CCWWGG 1 cut(s) 485
FaeI CATG 4 cut(s) 273, 322, 337, 397
FaiI YATR 5 cut(s) 271, 320, 335, 395, 476
FatI CATG 4 cut(s) 269, 318, 333, 393
Fnu4HI GCNGC 3 cut(s) 7, 38, 224
FokI GGATG 1 cut(s) 122
Fsp4HI GCNGC 3 cut(s) 7, 38, 224
FspBI CTAG 1 cut(s) 374
GlaI GCGC 1 cut(s) 36
GluI GCNGC 3 cut(s) 7, 38, 224
GsaI CCCAGC 1 cut(s) 525
GsuI CTGGAG 1 cut(s) 438
HaeII RGCGCY 1 cut(s) 38
HaeIII GGCC 2 cut(s) 51, 209
HapII CCGG 3 cut(s) 58, 243, 288
HhaI GCGC 1 cut(s) 37
Hin1II CATG 4 cut(s) 273, 322, 337, 397
Hin6I GCGC 1 cut(s) 35
HinP1I GCGC 1 cut(s) 35
HinfI GANTC 1 cut(s) 246
HpaII CCGG 3 cut(s) 58, 243, 288
HphI GGTGA 1 cut(s) 232
Hpy188I TCNGA 3 cut(s) 131, 348, 444
Hpy188III TCNNGA 5 cut(s) 288, 334, 374, 451, 506
HpyAV CCTTC 2 cut(s) 51, 310
HpyCH4III ACNGT 3 cut(s) 127, 158, 285
HpyCH4V TGCA 2 cut(s) 230, 545
HpyF10VI GCNNNNNNNGC 2 cut(s) 15, 206
Hsp92II CATG 4 cut(s) 273, 322, 337, 397
HspAI GCGC 1 cut(s) 35
Kpn2I TCCGGA 1 cut(s) 287
Kzo9I GATC 4 cut(s) 112, 466, 470, 502
LmnI GCTCC 1 cut(s) 197
Lsp1109I GCAGC 2 cut(s) 24, 235
LweI GCATC 1 cut(s) 144
MaeI CTAG 1 cut(s) 374
MaeIII GTNAC 1 cut(s) 238
MalI GATC 4 cut(s) 114, 468, 472, 504
MboI GATC 4 cut(s) 112, 466, 470, 502
MboII GAAGA 7 cut(s) 57, 60, 181, 272, 362, 394, 566
MfeI CAATTG 1 cut(s) 150
MhlI GDGCHC 1 cut(s) 436
MlsI TGGCCA 1 cut(s) 209
MluCI AATT 2 cut(s) 150, 186
MluNI TGGCCA 1 cut(s) 209
MmeI TCCRAC 2 cut(s) 109, 175
MnlI CCTC 3 cut(s) 94, 126, 250
Mox20I TGGCCA 1 cut(s) 209
MroI TCCGGA 1 cut(s) 287
MscI TGGCCA 1 cut(s) 209
MseI TTAA 1 cut(s) 278
Msp20I TGGCCA 1 cut(s) 209
MspI CCGG 3 cut(s) 58, 243, 288
MspR9I CCNGG 3 cut(s) 32, 58, 417
MunI CAATTG 1 cut(s) 150
MvaI CCWGG 2 cut(s) 32, 417
MwoI GCNNNNNNNGC 2 cut(s) 15, 206
NciI CCSGG 1 cut(s) 58
NdeII GATC 4 cut(s) 112, 466, 470, 502
NlaIII CATG 4 cut(s) 273, 322, 337, 397
NlaIV GGNNCC 1 cut(s) 435
NmeAIII GCCGAG 1 cut(s) 27
NmuCI GTSAC 1 cut(s) 238
PagI TCATGA 1 cut(s) 333
PfeI GAWTC 1 cut(s) 246
PfoI TCCNGGA 1 cut(s) 415
PkrI GCNGC 3 cut(s) 8, 39, 225
Psp6I CCWGG 2 cut(s) 30, 415
PspFI CCCAGC 1 cut(s) 521
PspGI CCWGG 2 cut(s) 30, 415
PspN4I GGNNCC 1 cut(s) 435
PsrI GAACNNNNNNTAC 2 cut(s) 374, 406
PstNI CAGNNNCTG 2 cut(s) 37, 551
RsaI GTAC 5 cut(s) 308, 359, 383, 482, 536
RsaNI GTAC 5 cut(s) 307, 358, 382, 481, 535
SaqAI TTAA 1 cut(s) 278
SatI GCNGC 3 cut(s) 7, 38, 224
Sau3AI GATC 4 cut(s) 112, 466, 470, 502
ScrFI CCNGG 3 cut(s) 32, 58, 417
SduI GDGCHC 1 cut(s) 436
SetI ASST 6 cut(s) 8, 20, 168, 356, 523, 553
SfaNI GCATC 1 cut(s) 144
Sse9I AATT 2 cut(s) 150, 186
SspMI CTAG 1 cut(s) 374
StyD4I CCNGG 3 cut(s) 30, 56, 415
StyI CCWWGG 1 cut(s) 485
TaaI ACNGT 3 cut(s) 127, 158, 285
TaqI TCGA 4 cut(s) 111, 176, 469, 491
TasI AATT 2 cut(s) 150, 186
TatI WGTACW 1 cut(s) 381
TfiI GAWTC 1 cut(s) 246
Tru1I TTAA 1 cut(s) 278
Tru9I TTAA 1 cut(s) 278
TseFI GTSAC 1 cut(s) 238
TseI GCWGC 3 cut(s) 6, 37, 223
Tsp45I GTSAC 1 cut(s) 238
TspDTI ATGAA 3 cut(s) 350, 382, 491
TspGWI ACGGA 1 cut(s) 128
XbaI TCTAGA 1 cut(s) 373
XcmI CCANNNNNNNNNTGG 1 cut(s) 518
XspI CTAG 1 cut(s) 374
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.