pycom16g02580

ELL-associated factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0011867
Physical Location & Seq
Reverse (-)
1707736 .. 1710017
2282 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1032 bp
ATGGCGGACAACTCCAAGGAAGAGCCCAAGACCGGCCCCCAGTCCAATCGCTGGTACAACCTAACCCTCGGCCCCTCCTTCAGAGACGGCTCCGCCAACAAGTACTGCACTCTCCGATACGACTTCAAGCCGGCGTCGATCGACAAGACTAAGGCCGGAGCACTGAAGAAGACCAAGGACAATAGGGTTACGGTCGAATTTCAGAACAACCAAGTGGGGAAACCCAATGTCACGTTTAAAGGGAGCAGCGAGGAGTACAAGGGCAACGACGCCGTATTATTCTTTGACGGCCAGACGTTTCGGTTGGAGAGGCTTCACCGCTCCGTGAAGCAGCTCCGGCATGACCGCCGGCCTGGGGAATCGGCGGCTGGTATGGCTGTAGACCCGCAGCTTTCTCCGGTTGGCAAGGCGCCCAAGCTAACGCATCAGTATAATCCTGCTAGAACTGCATTCCCCATCGTGCCGGTTGAGGTGGAACGGATAGATGTTGGGGTGCCTGAGAATCCAGGTGCGAAAGCTGCCAATAAAGGTGTGATTGATTACTCATCTGATCAACCAAATGTATCTGCTGCCTCGCCAGGCCCTAAGAATGAAGAAGTTAACGACGAACACCAGGATATAGATATTGAAGACCTTTTCGGTAGTTTATCACCCGATGATGGGAATGCTGCTGAAGATGAAGCCAATCCTGAATTTGACATCATTATGCCACATCAGAATGATAGTGATAATGAGATTGCTGATGTAGATGATAGCGGTGATGAAGTTGACAAGGGGCCAAATGCCGCAGAAGCCCTTCGAGCCCAGGTGATGAATGCACAGGGGAGGGAAACACACACTGATATGTTTGGAGACGAGGGGAATGCAGAAGGGAGGAAAACTGAGACTTCTAGTTCAAGTAGTAGCAGCGGAAGTAGCAGCGGAAGTGGGAGCAGCAGCAGCGGAAATAGTAGTGGGAGCAGCAGCAGCAGCGGCAGCGAAAATGGTGGTAATAATGCAGGCAGTGATGACGATTCAGTCAACTCCATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EAF

Protein Analysis

344

Amino Acids

36.51

Weight (kDa)

4.65

Isoelectric Point (pI)

46.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EAF PF09816 19 - 118 1.4e-23 RNA polymerase II transcription elongation factor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011867)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G71080
fragaria_vesca FvH4_4g32210 FvH4_4g32210 FvH4_7g01202
malus_domestica MD13G1027200.v1.1 MD16G1030300.v1.1
prunus_persica Prupe.1G322700_v2.0.a1
pyrus_communis pycom13g02590 pycom16g02580
rosa_chinensis RchiOBHm_Chr4g0440951
rosa_laevigata RLG00000006137
rosa_multiflora Rmu_sc0000424.1_g000025
rosa_roxburghii Rroxscaffold_5G00381810
rosa_rugosa Rorug04G0329600
rosa_samantha Rh4AG381400 Rh4BG394600 Rh4CG409500 Rh4DG387900
rosa_wichuraiana Rw4G032850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1016
AccB1I GGYRCC 2 cut(s) 409, 493
AccB7I CCANNNNNTGG 1 cut(s) 51
AccBSI CCGCTC 1 cut(s) 321
AccI GTMKAC 1 cut(s) 381
AcoI YGGCCR 1 cut(s) 289
AcsI RAATTY 2 cut(s) 197, 692
AcuI CTGAAG 3 cut(s) 64, 185, 693
AcyI GRCGYC 3 cut(s) 134, 270, 410
AfaI GTAC 3 cut(s) 56, 104, 257
AfiI CCNNNNNNNGG 5 cut(s) 32, 51, 355, 659, 660
AgsI TTSAA 3 cut(s) 127, 629, 897
AjnI CCWGG 5 cut(s) 352, 505, 577, 612, 804
AluBI AGCT 4 cut(s) 334, 391, 418, 518
AluI AGCT 4 cut(s) 334, 391, 418, 518
Alw21I GWGCWC 1 cut(s) 163
Alw26I GTCTC 3 cut(s) 78, 846, 878
AoxI GGCC 7 cut(s) 34, 70, 153, 289, 350, 580, 776
ApoI RAATTY 2 cut(s) 197, 692
Asp700I GAANNNNTTC 1 cut(s) 795
AspLEI GCGC 1 cut(s) 412
AspS9I GGNCC 4 cut(s) 35, 71, 581, 776
AsuHPI GGTGA 4 cut(s) 308, 642, 770, 820
BanI GGYRCC 2 cut(s) 409, 493
BanII GRGCYC 2 cut(s) 27, 805
BbsI GAAGAC 2 cut(s) 176, 636
Bbv12I GWGCWC 1 cut(s) 163
BccI CCATC 2 cut(s) 464, 653
BceAI ACGGC 3 cut(s) 103, 257, 304
BcgI CGANNNNNNTGC 2 cut(s) 845, 879
BciT130I CCWGG 5 cut(s) 354, 507, 579, 614, 806
BclI TGATCA 1 cut(s) 550
BcoDI GTCTC 3 cut(s) 78, 846, 878
BfaI CTAG 2 cut(s) 441, 891
BfmI CTRYAG 1 cut(s) 378
BfoI RGCGCY 1 cut(s) 413
BmcAI AGTACT 1 cut(s) 104
Bme1390I CCNGG 5 cut(s) 354, 507, 579, 614, 806
BmgT120I GGNCC 4 cut(s) 35, 71, 581, 776
BmiI GGNNCC 6 cut(s) 37, 73, 91, 411, 495, 777
BmrFI CCNGG 5 cut(s) 354, 507, 579, 614, 806
BmrI ACTGGG 1 cut(s) 34
BmsI GCATC 1 cut(s) 433
BmuI ACTGGG 1 cut(s) 34
BpiI GAAGAC 2 cut(s) 176, 636
BsaHI GRCGYC 3 cut(s) 134, 270, 410
BsaJI CCNNGG 5 cut(s) 15, 67, 174, 353, 804
BsaWI WCCGGW 1 cut(s) 397
Bsc4I CCNNNNNNNGG 5 cut(s) 32, 51, 355, 659, 660
Bse118I RCCGGY 4 cut(s) 32, 130, 348, 463
Bse1I ACTGG 1 cut(s) 40
BseBI CCWGG 5 cut(s) 354, 507, 579, 614, 806
BseDI CCNNGG 5 cut(s) 15, 67, 174, 353, 804
BseLI CCNNNNNNNGG 5 cut(s) 32, 51, 355, 659, 660
BseMII CTCAG 2 cut(s) 489, 873
BseNI ACTGG 1 cut(s) 40
BseRI GAGGAG 1 cut(s) 266
BsgI GTGCAG 1 cut(s) 91
Bsh1285I CGRYCG 2 cut(s) 141, 195
BshFI GGCC 7 cut(s) 36, 72, 155, 291, 352, 582, 778
BshNI GGYRCC 2 cut(s) 409, 493
BsiEI CGRYCG 2 cut(s) 141, 195
BsiHKAI GWGCWC 1 cut(s) 163
BsiSI CCGG 7 cut(s) 33, 131, 156, 337, 349, 398, 464
BslI CCNNNNNNNGG 5 cut(s) 32, 51, 355, 659, 660
BsmAI GTCTC 3 cut(s) 78, 846, 878
BsmBI CGTCTC 2 cut(s) 78, 846
BsmI GAATGC 4 cut(s) 449, 670, 820, 868
BsnI GGCC 7 cut(s) 36, 72, 155, 291, 352, 582, 778
Bsp1286I GDGCHC 3 cut(s) 27, 163, 805
Bsp143I GATC 2 cut(s) 138, 550
BspANI GGCC 7 cut(s) 36, 72, 155, 291, 352, 582, 778
BspCNI CTCAG 2 cut(s) 490, 874
BspLI GGNNCC 6 cut(s) 37, 73, 91, 411, 495, 777
BspQI GCTCTTC 1 cut(s) 15
BspT107I GGYRCC 2 cut(s) 409, 493
BsrBI CCGCTC 1 cut(s) 321
BsrFI RCCGGY 4 cut(s) 32, 130, 348, 463
BsrI ACTGG 1 cut(s) 40
BssAI RCCGGY 4 cut(s) 32, 130, 348, 463
BssECI CCNNGG 5 cut(s) 15, 67, 174, 353, 804
BssMI GATC 2 cut(s) 138, 550
BssNI GRCGYC 3 cut(s) 134, 270, 410
BssT1I CCWWGG 2 cut(s) 15, 174
Bst2UI CCWGG 5 cut(s) 354, 507, 579, 614, 806
Bst4CI ACNGT 1 cut(s) 193
Bst6I CTCTTC 1 cut(s) 15
BstACI GRCGYC 3 cut(s) 134, 270, 410
BstC8I GCNNGC 3 cut(s) 132, 350, 1000
BstDEI CTNAG 4 cut(s) 150, 498, 585, 882
BstH2I RGCGCY 1 cut(s) 413
BstHHI GCGC 1 cut(s) 412
BstKTI GATC 2 cut(s) 141, 553
BstMAI GTCTC 3 cut(s) 78, 846, 878
BstMBI GATC 2 cut(s) 138, 550
BstMCI CGRYCG 2 cut(s) 141, 195
BstNI CCWGG 5 cut(s) 354, 507, 579, 614, 806
BstSCI CCNGG 5 cut(s) 352, 505, 577, 612, 804
BstSFI CTRYAG 1 cut(s) 378
BstV2I GAAGAC 2 cut(s) 176, 636
BsuRI GGCC 7 cut(s) 36, 72, 155, 291, 352, 582, 778
BtsI GCAGTG 1 cut(s) 1009
BtsIMutI CAGTG 3 cut(s) 161, 837, 1009
Cac8I GCNNGC 3 cut(s) 132, 350, 1000
CfoI GCGC 1 cut(s) 412
Cfr10I RCCGGY 4 cut(s) 32, 130, 348, 463
Cfr13I GGNCC 4 cut(s) 35, 71, 581, 776
CseI GACGC 2 cut(s) 123, 278
Csp6I GTAC 3 cut(s) 55, 103, 256
CviAII CATG 1 cut(s) 341
CviQI GTAC 3 cut(s) 55, 103, 256
DdeI CTNAG 4 cut(s) 150, 498, 585, 882
DinI GGCGCC 1 cut(s) 411
DpnI GATC 2 cut(s) 140, 552
DpnII GATC 2 cut(s) 138, 550
DraI TTTAAA 1 cut(s) 238
DrdI GACNNNNNNGTC 1 cut(s) 1016
DseDI GACNNNNNNGTC 1 cut(s) 1016
EaeI YGGCCR 1 cut(s) 289
Eam1104I CTCTTC 1 cut(s) 15
EarI CTCTTC 1 cut(s) 15
EciI GGCGGA 2 cut(s) 20, 82
Eco130I CCWWGG 2 cut(s) 15, 174
Eco24I GRGCYC 2 cut(s) 27, 805
Eco57I CTGAAG 3 cut(s) 64, 185, 693
EcoO109I RGGNCCY 1 cut(s) 581
EcoRII CCWGG 5 cut(s) 352, 505, 577, 612, 804
EcoT14I CCWWGG 2 cut(s) 15, 174
EcoT38I GRGCYC 2 cut(s) 27, 805
EgeI GGCGCC 1 cut(s) 411
EheI GGCGCC 1 cut(s) 411
ErhI CCWWGG 2 cut(s) 15, 174
Esp3I CGTCTC 2 cut(s) 78, 846
FaeI CATG 1 cut(s) 344
FaiI YATR 6 cut(s) 342, 374, 432, 620, 707, 845
FatI CATG 1 cut(s) 340
FauI CCCGC 1 cut(s) 393
FbaI TGATCA 1 cut(s) 550
FblI GTMKAC 1 cut(s) 381
FriOI GRGCYC 2 cut(s) 27, 805
FspBI CTAG 2 cut(s) 441, 891
GlaI GCGC 1 cut(s) 411
HaeII RGCGCY 1 cut(s) 413
HaeIII GGCC 7 cut(s) 36, 72, 155, 291, 352, 582, 778
HapII CCGG 7 cut(s) 33, 131, 156, 337, 349, 398, 464
HgaI GACGC 2 cut(s) 123, 278
HhaI GCGC 1 cut(s) 412
Hin1I GRCGYC 3 cut(s) 134, 270, 410
Hin1II CATG 1 cut(s) 344
Hin6I GCGC 1 cut(s) 410
HinP1I GCGC 1 cut(s) 410
HincII GTYRAC 3 cut(s) 601, 769, 1021
HindII GTYRAC 3 cut(s) 601, 769, 1021
HinfI GANTC 3 cut(s) 359, 502, 1013
HpaI GTTAAC 1 cut(s) 601
HpaII CCGG 7 cut(s) 33, 131, 156, 337, 349, 398, 464
HphI GGTGA 4 cut(s) 308, 642, 770, 820
Hpy166II GTNNAC 4 cut(s) 382, 601, 769, 1021
Hpy188I TCNGA 6 cut(s) 83, 116, 204, 550, 717, 1031
Hpy188III TCNNGA 1 cut(s) 689
Hpy8I GTNNAC 4 cut(s) 382, 601, 769, 1021
Hpy99I CGWCG 3 cut(s) 139, 272, 608
HpyAV CCTTC 3 cut(s) 88, 806, 863
HpyCH4III ACNGT 1 cut(s) 193
HpyCH4IV ACGT 2 cut(s) 233, 296
HpyCH4V TGCA 5 cut(s) 108, 449, 818, 866, 998
HpyF3I CTNAG 4 cut(s) 150, 498, 585, 882
HpySE526I ACGT 2 cut(s) 233, 296
Hsp92I GRCGYC 3 cut(s) 134, 270, 410
Hsp92II CATG 1 cut(s) 344
HspAI GCGC 1 cut(s) 410
KasI GGCGCC 1 cut(s) 409
KroI GCCGGC 2 cut(s) 130, 348
KroNI GCCGGC 2 cut(s) 132, 350
Ksp22I TGATCA 1 cut(s) 550
KspAI GTTAAC 1 cut(s) 601
Kzo9I GATC 2 cut(s) 138, 550
LguI GCTCTTC 1 cut(s) 15
LmnI GCTCC 7 cut(s) 95, 158, 243, 326, 339, 930, 957
LweI GCATC 1 cut(s) 433
MaeI CTAG 2 cut(s) 441, 891
MaeII ACGT 2 cut(s) 233, 296
MaeIII GTNAC 2 cut(s) 187, 229
MalI GATC 2 cut(s) 140, 552
MbiI CCGCTC 1 cut(s) 321
MboI GATC 2 cut(s) 138, 550
MboII GAAGA 6 cut(s) 32, 178, 181, 605, 641, 686
MhlI GDGCHC 3 cut(s) 27, 163, 805
MluCI AATT 2 cut(s) 197, 692
Mly113I GGCGCC 1 cut(s) 410
MmeI TCCRAC 1 cut(s) 285
MnlI CCTC 9 cut(s) 77, 85, 244, 303, 463, 583, 819, 850, 867
MroNI GCCGGC 2 cut(s) 130, 348
MroXI GAANNNNTTC 1 cut(s) 795
MseI TTAA 2 cut(s) 237, 600
MslI CAYNNNNRTG 3 cut(s) 704, 717, 842
MspA1I CMGCKG 4 cut(s) 909, 921, 942, 972
MspI CCGG 7 cut(s) 33, 131, 156, 337, 349, 398, 464
MspR9I CCNGG 5 cut(s) 354, 507, 579, 614, 806
Mva1269I GAATGC 4 cut(s) 449, 670, 820, 868
MvaI CCWGG 5 cut(s) 354, 507, 579, 614, 806
NaeI GCCGGC 2 cut(s) 132, 350
NarI GGCGCC 1 cut(s) 410
NdeII GATC 2 cut(s) 138, 550
NgoMIV GCCGGC 2 cut(s) 130, 348
NlaIII CATG 1 cut(s) 344
NlaIV GGNNCC 6 cut(s) 37, 73, 91, 411, 495, 777
NmeAIII GCCGAG 1 cut(s) 48
NmuCI GTSAC 1 cut(s) 229
PciSI GCTCTTC 1 cut(s) 15
PctI GAATGC 4 cut(s) 449, 670, 820, 868
PdiI GCCGGC 2 cut(s) 132, 350
PdmI GAANNNNTTC 1 cut(s) 795
PfeI GAWTC 3 cut(s) 359, 502, 1013
PflMI CCANNNNNTGG 1 cut(s) 51
Ple19I CGATCG 1 cut(s) 141
PluTI GGCGCC 1 cut(s) 413
Psp6I CCWGG 5 cut(s) 352, 505, 577, 612, 804
PspGI CCWGG 5 cut(s) 352, 505, 577, 612, 804
PspN4I GGNNCC 6 cut(s) 37, 73, 91, 411, 495, 777
PspPI GGNCC 4 cut(s) 35, 71, 581, 776
PvuI CGATCG 1 cut(s) 141
RsaI GTAC 3 cut(s) 56, 104, 257
RsaNI GTAC 3 cut(s) 55, 103, 256
RseI CAYNNNNRTG 3 cut(s) 704, 717, 842
SapI GCTCTTC 1 cut(s) 15
SaqAI TTAA 2 cut(s) 237, 600
Sau3AI GATC 2 cut(s) 138, 550
Sau96I GGNCC 4 cut(s) 35, 71, 581, 776
ScaI AGTACT 1 cut(s) 104
ScrFI CCNGG 5 cut(s) 354, 507, 579, 614, 806
SduI GDGCHC 3 cut(s) 27, 163, 805
SfaNI GCATC 1 cut(s) 433
SfcI CTRYAG 1 cut(s) 378
SfoI GGCGCC 1 cut(s) 411
SmiMI CAYNNNNRTG 3 cut(s) 704, 717, 842
Sse9I AATT 2 cut(s) 197, 692
SspDI GGCGCC 1 cut(s) 409
SspMI CTAG 2 cut(s) 441, 891
StyD4I CCNGG 5 cut(s) 352, 505, 577, 612, 804
StyI CCWWGG 2 cut(s) 15, 174
TaaI ACNGT 1 cut(s) 193
TaiI ACGT 2 cut(s) 236, 299
TaqI TCGA 4 cut(s) 137, 141, 195, 799
TasI AATT 2 cut(s) 197, 692
TatI WGTACW 2 cut(s) 102, 255
TauI GCSGC 3 cut(s) 368, 788, 975
TfiI GAWTC 3 cut(s) 359, 502, 1013
Tru1I TTAA 2 cut(s) 237, 600
Tru9I TTAA 2 cut(s) 237, 600
TscAI CASTG 3 cut(s) 168, 844, 1009
TseFI GTSAC 1 cut(s) 229
Tsp45I GTSAC 1 cut(s) 229
TspDTI ATGAA 4 cut(s) 606, 693, 777, 827
TspGWI ACGGA 2 cut(s) 313, 493
TspRI CASTG 3 cut(s) 168, 844, 1009
Van91I CCANNNNNTGG 1 cut(s) 51
XapI RAATTY 2 cut(s) 197, 692
XmiI GTMKAC 1 cut(s) 381
XmnI GAANNNNTTC 1 cut(s) 795
XspI CTAG 2 cut(s) 441, 891
ZrmI AGTACT 1 cut(s) 104
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.