pycom16g03730

Homocysteine s-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0012188
Physical Location & Seq
Reverse (-)
2310754 .. 2312400
1647 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 981 bp
ATGGGATTGAAGAAGGCCATTACCTCGTCGTTGGAGGATGTAATAGAGAAAGCAGGGGGCTGTGCTGTGGTGGACGGAGGCTTCGCCACCCAGCTGGAGAGGCACGGCGCTGCCATCAACGACCCTCTCTGGAGCGCCGTCTGCCTCATCAAACAACCCGACCTCATCAAACGGGTTCACTTGGACTACTTGGAAGCCGGCGCTGATATTCTTATCACTTCTTCTTACCAGGCCACAATACCTGGATTTTTGTCCAGAGGACTCTCCATTGAACAAGGGGAGTTGTTGCTGAAGAAAAGTGTGAAACTGGCTGTCGAAGCCCGAAATAGTTTCTGGGATGCTCTGAAAGTGACACCCGATCATCGCTATAACCGAGCTTTGGTTGCTGCCTCCATTGGAAGCTATGGAGCTTATCTCGCTGACGGCTCAGAATATAGCGGGTGTTATGGACCCCGTGTGGATGTTGATAAGCTGAAGGATTTCCACCGGCGCAGATTGCAAGTTCTTGTCGAAGCAGGTCCTGATTTACTTGCATTTGAGACCATTCCTAATAAACTTGAAGCTCAGGCCTGCGTCGAGCTGCTGGACGAACAGAACGTCCAGATTCCATCTTGGATATGTTTCAGCTCTGTGGATGGTGAAAATGCCCCATCTGGAGAGAGTTTTAAGGAATGCCTTGAGGTGATAAGCAAAAGTAACAAAATACATGCTGTGGGAATAAATTGCACACCTCCTCATTTGATTCAATCTCTCATCTGCAAGTTTAAGGAGTTAACCAGTAAAGCAATTATTGTGTACCCTAATAGTGGTGAGATATGGGACGGCAAAGCTAAGAGATGGCTGCCTGCCAAGTGTTTTGATGATGAAAACTTTGAATGCTTTGCAACAATCTGGCGCGATTCGGGTGCTAAACTCATCGGAGGATGTTGTCGGACAACGCCTTCCACTGTTCAAGCCATTTCCAAGGTTCTAAAAGGATAG

Protein Analysis

327

Amino Acids

35.61

Weight (kDa)

6.12

Isoelectric Point (pI)

31.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S-methyl_trans PF02574 23 - 323 4.4e-78 Homocysteine S-methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012188)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G25900 AT3G25900 AT3G25900
fragaria_vesca FvH4_4g27410 FvH4_4g27410
malus_domestica MD16G1043200.v1.1
prunus_persica Prupe.1G310700_v2.0.a1
pyrus_communis pycom16g03730
rosa_chinensis RchiOBHm_Chr4g0435231
rosa_laevigata RLG00000006608
rosa_multiflora Rmu_ssc0000339.1_g000027
rosa_roxburghii Rroxscaffold_5G00376440
rosa_rugosa Rorug04G0283300 Rorug04G0283400
rosa_samantha Rh4AG338900 Rh4BG347100 Rh4CG361700 Rh4DG341500
rosa_wichuraiana Rw4G029560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 506
AccII CGCG 1 cut(s) 897
AciI CCGC 1 cut(s) 438
AcuI CTGAAG 2 cut(s) 311, 494
AfaI GTAC 1 cut(s) 797
AfiI CCNNNNNNNGG 2 cut(s) 379, 806
AgsI TTSAA 6 cut(s) 10, 272, 560, 746, 875, 953
AjnI CCWGG 2 cut(s) 228, 241
AloI GAACNNNNNNTCC 2 cut(s) 582, 614
AluBI AGCT 9 cut(s) 94, 377, 402, 410, 472, 563, 580, 627, 830
AluI AGCT 9 cut(s) 94, 377, 402, 410, 472, 563, 580, 627, 830
Alw26I GTCTC 1 cut(s) 533
AlwNI CAGNNNCTG 1 cut(s) 521
AoxI GGCC 3 cut(s) 15, 231, 567
ApeKI GCWGC 4 cut(s) 110, 386, 580, 841
ArsI GACNNNNNNTTYG 4 cut(s) 65, 97, 582, 614
Asp700I GAANNNNTTC 1 cut(s) 479
AspLEI GCGC 5 cut(s) 110, 137, 203, 492, 897
AspS9I GGNCC 2 cut(s) 449, 518
AsuHPI GGTGA 3 cut(s) 650, 694, 821
AvaII GGWCC 2 cut(s) 449, 518
BbvI GCAGC 4 cut(s) 97, 373, 567, 828
BccI CCATC 5 cut(s) 122, 616, 629, 658, 831
BceAI ACGGC 4 cut(s) 121, 122, 439, 838
BcgI CGANNNNNNTGC 2 cut(s) 887, 921
BciT130I CCWGG 2 cut(s) 230, 243
BcoDI GTCTC 1 cut(s) 533
BfoI RGCGCY 3 cut(s) 111, 138, 204
BfuAI ACCTGC 1 cut(s) 506
BisI GCNGC 4 cut(s) 111, 387, 581, 842
BlsI GCNGC 4 cut(s) 112, 388, 582, 843
Bme1390I CCNGG 2 cut(s) 230, 243
Bme18I GGWCC 2 cut(s) 449, 518
BmgT120I GGNCC 2 cut(s) 449, 518
BmiI GGNNCC 1 cut(s) 451
BmrFI CCNGG 2 cut(s) 230, 243
BmsI GCATC 1 cut(s) 328
BplI GAGNNNNNCTC 2 cut(s) 399, 431
BpmI CTGGAG 3 cut(s) 116, 151, 675
Bpu10I CCTNAGC 1 cut(s) 564
BpuEI CTTGAG 1 cut(s) 698
BsaI GGTCTC 1 cut(s) 533
BsaJI CCNNGG 1 cut(s) 963
Bsc4I CCNNNNNNNGG 2 cut(s) 379, 806
Bse118I RCCGGY 2 cut(s) 197, 486
Bse1I ACTGG 2 cut(s) 312, 777
BseBI CCWGG 2 cut(s) 230, 243
BseDI CCNNGG 1 cut(s) 963
BseGI GGATG 5 cut(s) 43, 343, 466, 640, 929
BseLI CCNNNNNNNGG 2 cut(s) 379, 806
BseMII CTCAG 2 cut(s) 441, 578
BseNI ACTGG 2 cut(s) 312, 777
BseRI GAGGAG 1 cut(s) 723
BseXI GCAGC 4 cut(s) 97, 373, 567, 828
BseYI CCCAGC 1 cut(s) 90
Bsh1236I CGCG 1 cut(s) 897
BshFI GGCC 3 cut(s) 17, 233, 569
BsiSI CCGG 2 cut(s) 198, 487
BslFI GGGAC 1 cut(s) 833
BslI CCNNNNNNNGG 2 cut(s) 379, 806
BsmAI GTCTC 1 cut(s) 533
BsmFI GGGAC 1 cut(s) 833
BsmI GAATGC 2 cut(s) 677, 881
BsnI GGCC 3 cut(s) 17, 233, 569
Bso31I GGTCTC 1 cut(s) 533
Bsp143I GATC 1 cut(s) 358
BspACI CCGC 1 cut(s) 438
BspANI GGCC 3 cut(s) 17, 233, 569
BspCNI CTCAG 2 cut(s) 440, 577
BspFNI CGCG 1 cut(s) 897
BspLI GGNNCC 1 cut(s) 451
BspMI ACCTGC 1 cut(s) 506
BspTNI GGTCTC 1 cut(s) 533
BsrFI RCCGGY 2 cut(s) 197, 486
BsrI ACTGG 2 cut(s) 312, 777
BssAI RCCGGY 2 cut(s) 197, 486
BssECI CCNNGG 1 cut(s) 963
BssMI GATC 1 cut(s) 358
BssT1I CCWWGG 1 cut(s) 963
Bst2UI CCWGG 2 cut(s) 230, 243
Bst4CI ACNGT 1 cut(s) 949
BstC8I GCNNGC 3 cut(s) 199, 571, 846
BstDEI CTNAG 3 cut(s) 427, 564, 831
BstF5I GGATG 5 cut(s) 43, 343, 466, 640, 929
BstFNI CGCG 1 cut(s) 897
BstH2I RGCGCY 3 cut(s) 111, 138, 204
BstHHI GCGC 5 cut(s) 110, 137, 203, 492, 897
BstKTI GATC 1 cut(s) 361
BstMAI GTCTC 1 cut(s) 533
BstMBI GATC 1 cut(s) 358
BstMWI GCNNNNNNNGC 6 cut(s) 100, 141, 317, 383, 416, 496
BstNI CCWGG 2 cut(s) 230, 243
BstNSI RCATGY 1 cut(s) 710
BstSCI CCNGG 2 cut(s) 228, 241
BstUI CGCG 1 cut(s) 897
BstV1I GCAGC 4 cut(s) 97, 373, 567, 828
BstXI CCANNNNNNTGG 1 cut(s) 94
BsuRI GGCC 3 cut(s) 17, 233, 569
BtgZI GCGATG 1 cut(s) 347
BtsCI GGATG 5 cut(s) 43, 343, 466, 640, 929
BtsIMutI CAGTG 1 cut(s) 945
BveI ACCTGC 1 cut(s) 506
Cac8I GCNNGC 3 cut(s) 199, 571, 846
CaiI CAGNNNCTG 1 cut(s) 521
CfoI GCGC 5 cut(s) 110, 137, 203, 492, 897
Cfr10I RCCGGY 2 cut(s) 197, 486
Cfr13I GGNCC 2 cut(s) 449, 518
CseI GACGC 1 cut(s) 562
Csp6I GTAC 1 cut(s) 796
CviAII CATG 1 cut(s) 707
CviQI GTAC 1 cut(s) 796
DdeI CTNAG 3 cut(s) 427, 564, 831
DpnI GATC 1 cut(s) 360
DpnII GATC 1 cut(s) 358
Eco130I CCWWGG 1 cut(s) 963
Eco147I AGGCCT 1 cut(s) 569
Eco31I GGTCTC 1 cut(s) 533
Eco47I GGWCC 2 cut(s) 449, 518
Eco57I CTGAAG 2 cut(s) 311, 494
EcoO109I RGGNCCY 1 cut(s) 518
EcoRII CCWGG 2 cut(s) 228, 241
EcoT14I CCWWGG 1 cut(s) 963
ErhI CCWWGG 1 cut(s) 963
FaeI CATG 1 cut(s) 710
FaiI YATR 7 cut(s) 369, 405, 435, 447, 619, 708, 817
FaqI GGGAC 1 cut(s) 833
FatI CATG 1 cut(s) 706
FauI CCCGC 1 cut(s) 431
Fnu4HI GCNGC 4 cut(s) 111, 387, 581, 842
FokI GGATG 5 cut(s) 50, 350, 473, 647, 936
Fsp4HI GCNGC 4 cut(s) 111, 387, 581, 842
GlaI GCGC 5 cut(s) 109, 136, 202, 491, 896
GluI GCNGC 4 cut(s) 111, 387, 581, 842
GsaI CCCAGC 1 cut(s) 94
GsuI CTGGAG 3 cut(s) 116, 151, 675
HaeII RGCGCY 3 cut(s) 111, 138, 204
HaeIII GGCC 3 cut(s) 17, 233, 569
HapII CCGG 2 cut(s) 198, 487
HgaI GACGC 1 cut(s) 562
HhaI GCGC 5 cut(s) 110, 137, 203, 492, 897
Hin1II CATG 1 cut(s) 710
Hin6I GCGC 5 cut(s) 108, 135, 201, 490, 895
HinP1I GCGC 5 cut(s) 108, 135, 201, 490, 895
HincII GTYRAC 1 cut(s) 774
HindII GTYRAC 1 cut(s) 774
HinfI GANTC 4 cut(s) 261, 604, 742, 899
HpaI GTTAAC 1 cut(s) 774
HpaII CCGG 2 cut(s) 198, 487
HphI GGTGA 3 cut(s) 650, 694, 821
Hpy166II GTNNAC 4 cut(s) 73, 178, 774, 796
Hpy188I TCNGA 4 cut(s) 345, 430, 920, 933
Hpy188III TCNNGA 5 cut(s) 130, 255, 521, 601, 654
Hpy8I GTNNAC 4 cut(s) 73, 178, 774, 796
Hpy99I CGWCG 2 cut(s) 31, 578
HpyAV CCTTC 3 cut(s) 7, 469, 951
HpyCH4III ACNGT 1 cut(s) 949
HpyCH4IV ACGT 1 cut(s) 597
HpyCH4V TGCA 5 cut(s) 499, 533, 726, 759, 884
HpyF10VI GCNNNNNNNGC 6 cut(s) 100, 141, 317, 383, 416, 496
HpyF3I CTNAG 3 cut(s) 427, 564, 831
HpySE526I ACGT 1 cut(s) 597
Hsp92II CATG 1 cut(s) 710
HspAI GCGC 5 cut(s) 108, 135, 201, 490, 895
KroI GCCGGC 1 cut(s) 197
KroNI GCCGGC 1 cut(s) 199
KspAI GTTAAC 1 cut(s) 774
Kzo9I GATC 1 cut(s) 358
LmnI GCTCC 2 cut(s) 132, 407
Lsp1109I GCAGC 4 cut(s) 97, 373, 567, 828
LweI GCATC 1 cut(s) 328
MaeII ACGT 1 cut(s) 597
MaeIII GTNAC 2 cut(s) 349, 695
MalI GATC 1 cut(s) 360
MboI GATC 1 cut(s) 358
MboII GAAGA 3 cut(s) 22, 213, 304
MluCI AATT 2 cut(s) 721, 786
MlyI GAGTC 1 cut(s) 255
MmeI TCCRAC 2 cut(s) 12, 911
MroNI GCCGGC 1 cut(s) 197
MroXI GAANNNNTTC 1 cut(s) 479
MseI TTAA 3 cut(s) 666, 765, 773
MspA1I CMGCKG 1 cut(s) 94
MspI CCGG 2 cut(s) 198, 487
MspR9I CCNGG 2 cut(s) 230, 243
Mva1269I GAATGC 2 cut(s) 677, 881
MvaI CCWGG 2 cut(s) 230, 243
MvnI CGCG 1 cut(s) 897
MwoI GCNNNNNNNGC 6 cut(s) 100, 141, 317, 383, 416, 496
NaeI GCCGGC 1 cut(s) 199
NdeII GATC 1 cut(s) 358
NgoMIV GCCGGC 1 cut(s) 197
NlaIII CATG 1 cut(s) 710
NlaIV GGNNCC 1 cut(s) 451
NmuCI GTSAC 1 cut(s) 349
NspI RCATGY 1 cut(s) 710
PceI AGGCCT 1 cut(s) 569
PcsI WCGNNNNNNNCGW 2 cut(s) 370, 594
PctI GAATGC 2 cut(s) 677, 881
PdiI GCCGGC 1 cut(s) 199
PdmI GAANNNNTTC 1 cut(s) 479
PfeI GAWTC 3 cut(s) 604, 742, 899
PkrI GCNGC 4 cut(s) 112, 388, 582, 843
PleI GAGTC 1 cut(s) 255
PpsI GAGTC 1 cut(s) 255
PpuMI RGGWCCY 1 cut(s) 518
Psp5II RGGWCCY 1 cut(s) 518
Psp6I CCWGG 2 cut(s) 228, 241
PspFI CCCAGC 1 cut(s) 90
PspGI CCWGG 2 cut(s) 228, 241
PspN4I GGNNCC 1 cut(s) 451
PspPI GGNCC 2 cut(s) 449, 518
PspPPI RGGWCCY 1 cut(s) 518
PstNI CAGNNNCTG 1 cut(s) 521
PvuII CAGCTG 1 cut(s) 94
RsaI GTAC 1 cut(s) 797
RsaNI GTAC 1 cut(s) 796
SaqAI TTAA 3 cut(s) 666, 765, 773
SatI GCNGC 4 cut(s) 111, 387, 581, 842
Sau3AI GATC 1 cut(s) 358
Sau96I GGNCC 2 cut(s) 449, 518
SchI GAGTC 1 cut(s) 255
ScrFI CCNGG 2 cut(s) 230, 243
SfaNI GCATC 1 cut(s) 328
SgrAI CRCCGGYG 1 cut(s) 486
SinI GGWCC 2 cut(s) 449, 518
SmlI CTYRAG 1 cut(s) 677
SmoI CTYRAG 1 cut(s) 677
Sse9I AATT 2 cut(s) 721, 786
SseBI AGGCCT 1 cut(s) 569
SsiI CCGC 1 cut(s) 438
StuI AGGCCT 1 cut(s) 569
StyD4I CCNGG 2 cut(s) 228, 241
StyI CCWWGG 1 cut(s) 963
TaaI ACNGT 1 cut(s) 949
TaiI ACGT 1 cut(s) 600
TaqI TCGA 3 cut(s) 315, 510, 576
TasI AATT 2 cut(s) 721, 786
TfiI GAWTC 3 cut(s) 604, 742, 899
Tru1I TTAA 3 cut(s) 666, 765, 773
Tru9I TTAA 3 cut(s) 666, 765, 773
TscAI CASTG 1 cut(s) 952
TseFI GTSAC 1 cut(s) 349
TseI GCWGC 4 cut(s) 110, 386, 580, 841
Tsp45I GTSAC 1 cut(s) 349
TspDTI ATGAA 1 cut(s) 879
TspGWI ACGGA 1 cut(s) 90
TspRI CASTG 1 cut(s) 952
VpaK11BI GGWCC 2 cut(s) 449, 518
XceI RCATGY 1 cut(s) 710
XmnI GAANNNNTTC 1 cut(s) 479
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.