pycom16g04110

mediator of RNA polymerase II transcription subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0005569
Physical Location & Seq
Reverse (-)
2527541 .. 2534883
7343 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2487 bp
ATGGCTGAGAAACAACTGATCATCGCTGTTGAAGGGACTGCAGCCATGGGTCCCTTCTGGGAACTCATTTTTAAAGATTATTTAGAGAAGATCGTAAGGAGTTTCTGTGGAAACGAGCCAAGTGGGCAGAAGCTCTCGACTTCTACTGTTGAGCTCTCCCTGGTCACATTCAATACTCATGGATCTTATTGTGGTTGCCTGGTACAACGGAGTGGCTGGACAAGGGATGTGGATCTCTTCTTACATTGGCTTTCAGCTATACCCTTTTCTGGGGGTGGGTTTAGTGATGCTGCAATTGCGGAAGGGCTTTCGGAAGCTCTAATGATGTTCCCTACAGTTCAAAATGGAAGCCAAAATCAACAAAATGTGGATTTTCAGAAGCATTGTGTTCTTATTGCTGCGAGTAACCCTCACCCTCTGCCCACACCAGTGTATCGGCCACAAATTCAAAATTTGGAGCAAAATGAAAATATTGATGCACAAACTGAAAATCGTCTTTATGATGCTGAGGCTGTTGCTAAATCATTTCCACAGAGCTCTGTTTCTCTGTCAGTCATTTGTCCAAAACAGCTACCTAAGCTCAGAGCAATATACAATGCAGGAAAGCGCAACCCTAGAGCGGCAGATCCACCAATTGATAATGCGAAAAATCCCCACTTTATTGTTTTAATCTCAGAGAATTTTATGGAGGCCCGTGCTACTTTAAGTCGTCCGGGAATTACAACTTTGCCCTCAAATCAGAGTCCTGTAAAAATGGACATAGCTCCTGTTGCTTCAGTTACTGGGCCGCCTCCAACTTCTATGCCCTCAGTGAATGGACCTGTTATGAATCGGCAACCAATTTCTGTTGGAAATGTGCCTCCTGCTACTGTAAAAGTTGAGCCAAGTACCATAAGTTCCATGGGACCTTCTTTTGCACACATTCCATCTGTCCGTCCTCCTTCCCAAGGAGTCCCAAGCTTGCAGACTTCGTCACCATCTTCTGCTTCGCAAGAGATGGCCACAAATAATGATAATGTGCCAGATTTGAAACCTGTAGTGAGTGGTGTGACACATCCCTCGCGTCCTGTGGGTAGCATCCTCAATAACATCTCTCAAGCACGTGTGATGAACGCAGCAGCCTTAACTGGAGGAACTTCTATGGGACTCCCAACTATGGGTCAAAATCCTATGGCCATGCATGTATCAAACATGATATCTAGTGGAATGGCATCTTCAGTTGGTGCTGCTCAAAATGTATTTTCATCTTCAGGGTCCGGCAATCTTACACAGGTTGCGCAGAACCCAGGTCTTAATTCATTCACTTCAGGTACCTCTAATGTCTCTGGCAACAATAACCTTGGGATGTCACAACCAATGAGTAACCTCCAAGGTGGTGTTAGTATGGGTCAAGCTGTTCCTGGTATGAGCCAAGGAAATCTCTCGGGACCGCAAATGGTTCAAAGTGGAATTGGCATGAACCCAAACATGATGAGTGCCCTTGGTTCAACAGGTGCCTCTTCTGGAACTGGTACAATGATTCCTACTCCAGGAATGGCTCAACAGGTGCAATCTGGGATGCAGTCACTTGGTGCAAACAATAATTCAGCATCTAATGTGCCGTTTTCACAACAGACATCAAGTGCTCTGGCATCTGCACAATCCAAATATGTGAAAGTCTGGGAGGGAAATTTATCTGGGCAGAGACAAGGGCAGCCTGTGTTTATCACTAGATTGGAGGGTTACAGGAGTGCTTCAGCTTCTGAGACGCTTGCAGCAAACTGGCCACCAACAATGCAAATAGTGAGGCTCATATCTCAGGACCACATGAATAACAAGCAATATGTTGGAAAGGCAGACTTTTTAGTTTTTCGGGCAATGAATCAGCATGGGTTTCTTGGGCAATTGCAAGAGAAGAAACTTTGTGCAGTGATCCAATTGCCCTCTCAGACACTACTGCTCTCTGTTTCAGACAAAGCATGCCGCTTGATTGGAATGCTGTTTCCAGGGGATATGGTTGTGTTTAAGCCACAAGTAGGCGGTCAGCAACAGCAGCAGCAAATGCAACAGCTACAACAGCAACAAATTCAAGTAGGTGGTCAGCAACAACACATGCAAGTTCCCGGTCAGCAACAGCAGCAACAAATGCAAGTACCCGGTCAGCAACAACAGCAACAAATGCAAGTACCCGGTCAGCAACAGCAGCAACAAATGCAAGTACCCGGTCAGCAACAACAGCATCCTCAGATTCAACAACAACATCCACAGATTCAACAGCAGCACCCTCAGATTCAACAACAGCAGCAACTCTCGCAGCAGCAGCTCTCACAACTTCAATCACAACAGCAACTGCCGCAGCTTCAGCAATTGCAGCAACAGCATCAACAACAACAGTTGGTTGGGACAGGGATGACTCAAGCTTATGTTCAAGGCCGGTCGCAATTAGTCTCACAGGGACAGGTTTCGTCACAAGGGCCTAATATGCCTGGAGGGGGCTTTATGGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Med25_VWA

Protein Analysis

829

Amino Acids

88.74

Weight (kDa)

8.87

Isoelectric Point (pI)

55.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Med25_VWA PF11265 3 - 226 1.9e-42 Mediator complex subunit 25 von Willebrand factor type A
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1278
Acc65I GGTACC 1 cut(s) 1310
AccB1I GGYRCC 2 cut(s) 1310, 1493
AccBSI CCGCTC 1 cut(s) 620
AccII CGCG 1 cut(s) 1063
AciI CCGC 7 cut(s) 299, 620, 788, 1430, 1963, 2019, 2333
AclWI GGATC 4 cut(s) 190, 240, 620, 1906
AcoI YGGCCR 4 cut(s) 437, 999, 1173, 1763
AcsI RAATTY 5 cut(s) 444, 451, 679, 1669, 2064
AcuI CTGAAG 6 cut(s) 759, 1200, 1233, 1290, 1719, 2324
AcvI CACGTG 1 cut(s) 1103
AdeI CACNNNGTG 1 cut(s) 1571
AfaI GTAC 7 cut(s) 204, 889, 1312, 1513, 2133, 2166, 2199
AfiI CCNNNNNNNGG 9 cut(s) 269, 270, 619, 947, 1156, 1157, 1529, 2015, 2471
AflIII ACRYGT 1 cut(s) 1102
AjnI CCWGG 7 cut(s) 159, 198, 1285, 1399, 1528, 1984, 2464
AleI CACNNNNGTG 1 cut(s) 428
Alw21I GWGCWC 3 cut(s) 156, 539, 1627
Alw26I GTCTC 4 cut(s) 1327, 1678, 1739, 2431
AlwI GGATC 4 cut(s) 190, 240, 620, 1906
AlwNI CAGNNNCTG 3 cut(s) 782, 1742, 2329
Ama87I CYCGRG 1 cut(s) 1423
AoxI GGCC 8 cut(s) 437, 690, 785, 999, 1173, 1763, 2410, 2453
ApoI RAATTY 5 cut(s) 444, 451, 679, 1669, 2064
Asp718I GGTACC 1 cut(s) 1310
AspLEI GCGC 2 cut(s) 609, 1279
AspS9I GGNCC 9 cut(s) 50, 691, 785, 818, 905, 1254, 1427, 1801, 2453
AsuC2I CCSGG 5 cut(s) 714, 2103, 2136, 2169, 2202
AsuHPI GGTGA 2 cut(s) 404, 966
AvaI CYCGRG 1 cut(s) 1423
AvaII GGWCC 6 cut(s) 50, 818, 905, 1254, 1427, 1801
BaeGI GKGCMC 1 cut(s) 1480
BaeI ACNNNNGTAYC 2 cut(s) 416, 449
BalI TGGCCA 3 cut(s) 1001, 1175, 1765
BanI GGYRCC 2 cut(s) 1310, 1493
BanII GRGCYC 2 cut(s) 156, 539
BbrPI CACGTG 1 cut(s) 1103
Bbv12I GWGCWC 3 cut(s) 156, 539, 1627
BbvCI CCTCAGC 1 cut(s) 507
BccI CCATC 3 cut(s) 934, 985, 991
BceAI ACGGC 1 cut(s) 1585
BciT130I CCWGG 7 cut(s) 161, 200, 1287, 1401, 1530, 1986, 2466
BclI TGATCA 1 cut(s) 18
BcnI CCSGG 5 cut(s) 714, 2103, 2136, 2169, 2202
BcoDI GTCTC 4 cut(s) 1327, 1678, 1739, 2431
BfaI CTAG 3 cut(s) 615, 1200, 1710
BfmI CTRYAG 3 cut(s) 39, 333, 1035
BglI GCCNNNNNGGC 1 cut(s) 124
Bme18I GGWCC 6 cut(s) 50, 818, 905, 1254, 1427, 1801
BmeT110I CYCGRG 1 cut(s) 1423
BmgT120I GGNCC 9 cut(s) 50, 691, 785, 818, 905, 1254, 1427, 1801, 2453
BmiI GGNNCC 7 cut(s) 51, 52, 906, 1255, 1312, 1428, 1495
BmrI ACTGGG 1 cut(s) 792
BmuI ACTGGG 1 cut(s) 792
BplI GAGNNNNNCTC 2 cut(s) 394, 426
BpmI CTGGAG 3 cut(s) 1149, 1512, 2487
Bpu10I CCTNAGC 2 cut(s) 507, 576
BpuEI CTTGAG 2 cut(s) 1080, 2379
BpuMI CCSGG 5 cut(s) 714, 2103, 2136, 2169, 2202
BsaAI YACGTR 1 cut(s) 1103
BsaBI GATNNNNATC 1 cut(s) 231
BsaXI ACNNNNNCTCC 2 cut(s) 91, 121
Bsc4I CCNNNNNNNGG 9 cut(s) 269, 270, 619, 947, 1156, 1157, 1529, 2015, 2471
Bse118I RCCGGY 1 cut(s) 2412
Bse1I ACTGG 5 cut(s) 428, 787, 1132, 1513, 1766
Bse3DI GCAATG 1 cut(s) 1863
Bse8I GATNNNNATC 1 cut(s) 231
BseBI CCWGG 7 cut(s) 161, 200, 1287, 1401, 1530, 1986, 2466
BseGI GGATG 8 cut(s) 232, 1054, 1077, 1350, 1563, 2218, 2239, 2394
BseJI GATNNNNATC 1 cut(s) 231
BseLI CCNNNNNNNGG 9 cut(s) 269, 270, 619, 947, 1156, 1157, 1529, 2015, 2471
BseMI GCAATG 1 cut(s) 1863
BseMII CTCAG 9 cut(s) 498, 595, 687, 822, 1734, 1811, 1940, 2237, 2279
BseNI ACTGG 5 cut(s) 428, 787, 1132, 1513, 1766
BseSI GKGCMC 1 cut(s) 1480
BsgI GTGCAG 2 cut(s) 1620, 1926
Bsh1236I CGCG 1 cut(s) 1063
Bsh1285I CGRYCG 1 cut(s) 2417
BshFI GGCC 8 cut(s) 439, 692, 787, 1001, 1175, 1765, 2412, 2455
BshNI GGYRCC 2 cut(s) 1310, 1493
BsiEI CGRYCG 1 cut(s) 2417
BsiHKAI GWGCWC 3 cut(s) 156, 539, 1627
BsiHKCI CYCGRG 1 cut(s) 1423
BsiSI CCGG 7 cut(s) 713, 1257, 2103, 2136, 2169, 2202, 2413
BslFI GGGAC 8 cut(s) 36, 49, 918, 938, 1158, 1440, 2395, 2448
BslI CCNNNNNNNGG 9 cut(s) 269, 270, 619, 947, 1156, 1157, 1529, 2015, 2471
BsmAI GTCTC 4 cut(s) 1327, 1678, 1739, 2431
BsmBI CGTCTC 1 cut(s) 1739
BsmFI GGGAC 8 cut(s) 36, 49, 918, 938, 1158, 1440, 2395, 2448
BsmI GAATGC 1 cut(s) 1980
BsnI GGCC 8 cut(s) 439, 692, 787, 1001, 1175, 1765, 2412, 2455
BsoBI CYCGRG 1 cut(s) 1423
Bsp1286I GDGCHC 4 cut(s) 156, 539, 1480, 1627
Bsp143I GATC 6 cut(s) 18, 90, 182, 232, 625, 1911
Bsp19I CCATGG 2 cut(s) 45, 900
BspACI CCGC 7 cut(s) 299, 620, 788, 1430, 1963, 2019, 2333
BspANI GGCC 8 cut(s) 439, 692, 787, 1001, 1175, 1765, 2412, 2455
BspCNI CTCAG 9 cut(s) 499, 594, 686, 821, 1735, 1810, 1939, 2236, 2278
BspFNI CGCG 1 cut(s) 1063
BspLI GGNNCC 7 cut(s) 51, 52, 906, 1255, 1312, 1428, 1495
BspMAI CTGCAG 1 cut(s) 43
BspPI GGATC 4 cut(s) 190, 240, 620, 1906
BspT107I GGYRCC 2 cut(s) 1310, 1493
BsrBI CCGCTC 1 cut(s) 620
BsrDI GCAATG 1 cut(s) 1863
BsrFI RCCGGY 1 cut(s) 2412
BsrI ACTGG 5 cut(s) 428, 787, 1132, 1513, 1766
BssAI RCCGGY 1 cut(s) 2412
BssMI GATC 6 cut(s) 18, 90, 182, 232, 625, 1911
BssT1I CCWWGG 7 cut(s) 45, 900, 946, 1339, 1369, 1411, 1480
Bst2UI CCWGG 7 cut(s) 161, 200, 1287, 1401, 1530, 1986, 2466
Bst4CI ACNGT 4 cut(s) 148, 337, 871, 2373
Bst6I CTCTTC 2 cut(s) 242, 1504
BstAPI GCANNNNNTGC 4 cut(s) 2041, 2125, 2158, 2191
BstBAI YACGTR 1 cut(s) 1103
BstC8I GCNNGC 3 cut(s) 962, 1752, 1960
BstDSI CCRYGG 2 cut(s) 45, 900
BstENI CCTNNNNNAGG 2 cut(s) 945, 1527
BstF5I GGATG 8 cut(s) 232, 1054, 1077, 1350, 1563, 2218, 2239, 2394
BstFNI CGCG 1 cut(s) 1063
BstHHI GCGC 2 cut(s) 609, 1279
BstKTI GATC 6 cut(s) 21, 93, 185, 235, 628, 1914
BstMAI GTCTC 4 cut(s) 1327, 1678, 1739, 2431
BstMBI GATC 6 cut(s) 18, 90, 182, 232, 625, 1911
BstMCI CGRYCG 1 cut(s) 2417
BstNI CCWGG 7 cut(s) 161, 200, 1287, 1401, 1530, 1986, 2466
BstNSI RCATGY 3 cut(s) 1184, 1962, 2095
BstSFI CTRYAG 3 cut(s) 39, 333, 1035
BstSLI GKGCMC 1 cut(s) 1480
BstUI CGCG 1 cut(s) 1063
BstX2I RGATCY 3 cut(s) 182, 232, 625
BstYI RGATCY 3 cut(s) 182, 232, 625
BsuRI GGCC 8 cut(s) 439, 692, 787, 1001, 1175, 1765, 2412, 2455
BtgI CCRYGG 2 cut(s) 45, 900
BtgZI GCGATG 1 cut(s) 7
BtsCI GGATG 8 cut(s) 232, 1054, 1077, 1350, 1563, 2218, 2239, 2394
BtsI GCAGTG 1 cut(s) 1914
BtsIMutI CAGTG 3 cut(s) 435, 816, 1914
Cac8I GCNNGC 3 cut(s) 962, 1752, 1960
CaiI CAGNNNCTG 3 cut(s) 782, 1742, 2329
CfoI GCGC 2 cut(s) 609, 1279
Cfr10I RCCGGY 1 cut(s) 2412
Cfr13I GGNCC 9 cut(s) 50, 691, 785, 818, 905, 1254, 1427, 1801, 2453
CseI GACGC 2 cut(s) 1052, 1756
Csp6I GTAC 7 cut(s) 203, 888, 1311, 1512, 2132, 2165, 2198
CspCI CAANNNNNGTGG 2 cut(s) 210, 245
CviQI GTAC 7 cut(s) 203, 888, 1311, 1512, 2132, 2165, 2198
DpnI GATC 6 cut(s) 20, 92, 184, 234, 627, 1913
DpnII GATC 6 cut(s) 18, 90, 182, 232, 625, 1911
DraI TTTAAA 1 cut(s) 73
DraIII CACNNNGTG 1 cut(s) 1571
EaeI YGGCCR 4 cut(s) 437, 999, 1173, 1763
Eam1104I CTCTTC 2 cut(s) 242, 1504
EarI CTCTTC 2 cut(s) 242, 1504
Ecl136II GAGCTC 2 cut(s) 154, 537
Eco130I CCWWGG 7 cut(s) 45, 900, 946, 1339, 1369, 1411, 1480
Eco24I GRGCYC 2 cut(s) 156, 539
Eco32I GATATC 1 cut(s) 1197
Eco47I GGWCC 6 cut(s) 50, 818, 905, 1254, 1427, 1801
Eco53kI GAGCTC 2 cut(s) 154, 537
Eco57I CTGAAG 6 cut(s) 759, 1200, 1233, 1290, 1719, 2324
Eco72I CACGTG 1 cut(s) 1103
Eco88I CYCGRG 1 cut(s) 1423
EcoICRI GAGCTC 2 cut(s) 154, 537
EcoNI CCTNNNNNAGG 2 cut(s) 945, 1527
EcoO109I RGGNCCY 3 cut(s) 50, 905, 2453
EcoRII CCWGG 7 cut(s) 159, 198, 1285, 1399, 1528, 1984, 2464
EcoRV GATATC 1 cut(s) 1197
EcoT14I CCWWGG 7 cut(s) 45, 900, 946, 1339, 1369, 1411, 1480
EcoT22I ATGCAT 1 cut(s) 1182
EcoT38I GRGCYC 2 cut(s) 156, 539
ErhI CCWWGG 7 cut(s) 45, 900, 946, 1339, 1369, 1411, 1480
Esp3I CGTCTC 1 cut(s) 1739
FalI AAGNNNNNCTT 2 cut(s) 1823, 1855
FaqI GGGAC 8 cut(s) 36, 49, 918, 938, 1158, 1440, 2395, 2448
FbaI TGATCA 1 cut(s) 18
FokI GGATG 8 cut(s) 239, 1041, 1064, 1357, 1570, 2205, 2226, 2401
FriOI GRGCYC 2 cut(s) 156, 539
FspBI CTAG 3 cut(s) 615, 1200, 1710
FspI TGCGCA 1 cut(s) 1278
GlaI GCGC 2 cut(s) 608, 1278
GsuI CTGGAG 3 cut(s) 1149, 1512, 2487
HaeIII GGCC 8 cut(s) 439, 692, 787, 1001, 1175, 1765, 2412, 2455
HapII CCGG 7 cut(s) 713, 1257, 2103, 2136, 2169, 2202, 2413
HgaI GACGC 2 cut(s) 1052, 1756
HhaI GCGC 2 cut(s) 609, 1279
Hin6I GCGC 2 cut(s) 607, 1277
HinP1I GCGC 2 cut(s) 607, 1277
HindIII AAGCTT 2 cut(s) 958, 2397
HpaII CCGG 7 cut(s) 713, 1257, 2103, 2136, 2169, 2202, 2413
HphI GGTGA 2 cut(s) 404, 966
Hpy188III TCNNGA 4 cut(s) 136, 1425, 1503, 1799
HpyAV CCTTC 5 cut(s) 26, 64, 296, 918, 951
HpyCH4III ACNGT 4 cut(s) 148, 337, 871, 2373
HpyCH4IV ACGT 1 cut(s) 1102
HpySE526I ACGT 1 cut(s) 1102
HspAI GCGC 2 cut(s) 607, 1277
KflI GGGWCCC 1 cut(s) 50
KpnI GGTACC 1 cut(s) 1314
Ksp22I TGATCA 1 cut(s) 18
Kzo9I GATC 6 cut(s) 18, 90, 182, 232, 625, 1911
LmnI GCTCC 2 cut(s) 457, 769
MaeI CTAG 3 cut(s) 615, 1200, 1710
MaeII ACGT 1 cut(s) 1102
MalI GATC 6 cut(s) 20, 92, 184, 234, 627, 1913
MbiI CCGCTC 1 cut(s) 620
MboI GATC 6 cut(s) 18, 90, 182, 232, 625, 1911
MboII GAAGA 7 cut(s) 100, 229, 972, 1206, 1239, 1491, 1906
MfeI CAATTG 5 cut(s) 294, 633, 1883, 1916, 2345
MflI RGATCY 3 cut(s) 182, 232, 625
MhlI GDGCHC 4 cut(s) 156, 539, 1480, 1627
MlsI TGGCCA 3 cut(s) 1001, 1175, 1765
MluNI TGGCCA 3 cut(s) 1001, 1175, 1765
MlyI GAGTC 4 cut(s) 751, 960, 1140, 2386
MmeI TCCRAC 3 cut(s) 818, 829, 1807
Mox20I TGGCCA 3 cut(s) 1001, 1175, 1765
Mph1103I ATGCAT 1 cut(s) 1182
MscI TGGCCA 3 cut(s) 1001, 1175, 1765
MseI TTAA 7 cut(s) 72, 668, 704, 1124, 1293, 2004, 2485
MslI CAYNNNNRTG 1 cut(s) 428
Msp20I TGGCCA 3 cut(s) 1001, 1175, 1765
MspI CCGG 7 cut(s) 713, 1257, 2103, 2136, 2169, 2202, 2413
MunI CAATTG 5 cut(s) 294, 633, 1883, 1916, 2345
Mva1269I GAATGC 1 cut(s) 1980
MvaI CCWGG 7 cut(s) 161, 200, 1287, 1401, 1530, 1986, 2466
MvnI CGCG 1 cut(s) 1063
NciI CCSGG 5 cut(s) 714, 2103, 2136, 2169, 2202
NcoI CCATGG 2 cut(s) 45, 900
NdeII GATC 6 cut(s) 18, 90, 182, 232, 625, 1911
NlaIV GGNNCC 7 cut(s) 51, 52, 906, 1255, 1312, 1428, 1495
NmuCI GTSAC 6 cut(s) 163, 972, 1048, 1347, 1563, 2445
NsbI TGCGCA 1 cut(s) 1278
NsiI ATGCAT 1 cut(s) 1182
NspI RCATGY 3 cut(s) 1184, 1962, 2095
OliI CACNNNNGTG 1 cut(s) 428
PaeI GCATGC 1 cut(s) 1962
PctI GAATGC 1 cut(s) 1980
PfeI GAWTC 6 cut(s) 829, 1519, 1861, 2227, 2248, 2269
PflFI GACNNNGTC 1 cut(s) 970
PfoI TCCNGGA 2 cut(s) 712, 1528
PleI GAGTC 4 cut(s) 750, 959, 1140, 2386
PmaCI CACGTG 1 cut(s) 1103
PmlI CACGTG 1 cut(s) 1103
PpsI GAGTC 4 cut(s) 750, 959, 1140, 2386
Ppu21I YACGTR 1 cut(s) 1103
PpuMI RGGWCCY 2 cut(s) 50, 905
Psp124BI GAGCTC 2 cut(s) 156, 539
Psp5II RGGWCCY 2 cut(s) 50, 905
Psp6I CCWGG 7 cut(s) 159, 198, 1285, 1399, 1528, 1984, 2464
PspCI CACGTG 1 cut(s) 1103
PspGI CCWGG 7 cut(s) 159, 198, 1285, 1399, 1528, 1984, 2464
PspN4I GGNNCC 7 cut(s) 51, 52, 906, 1255, 1312, 1428, 1495
PspPI GGNCC 9 cut(s) 50, 691, 785, 818, 905, 1254, 1427, 1801, 2453
PspPPI RGGWCCY 2 cut(s) 50, 905
PsrI GAACNNNNNNTAC 2 cut(s) 880, 912
PstI CTGCAG 1 cut(s) 43
PstNI CAGNNNCTG 3 cut(s) 782, 1742, 2329
PsuI RGATCY 3 cut(s) 182, 232, 625
PsyI GACNNNGTC 1 cut(s) 970
RsaI GTAC 7 cut(s) 204, 889, 1312, 1513, 2133, 2166, 2199
RsaNI GTAC 7 cut(s) 203, 888, 1311, 1512, 2132, 2165, 2198
RseI CAYNNNNRTG 1 cut(s) 428
SacI GAGCTC 2 cut(s) 156, 539
SaqAI TTAA 7 cut(s) 72, 668, 704, 1124, 1293, 2004, 2485
Sau3AI GATC 6 cut(s) 18, 90, 182, 232, 625, 1911
Sau96I GGNCC 9 cut(s) 50, 691, 785, 818, 905, 1254, 1427, 1801, 2453
SchI GAGTC 4 cut(s) 751, 960, 1140, 2386
SduI GDGCHC 4 cut(s) 156, 539, 1480, 1627
SfcI CTRYAG 3 cut(s) 39, 333, 1035
SinI GGWCC 6 cut(s) 50, 818, 905, 1254, 1427, 1801
SmiMI CAYNNNNRTG 1 cut(s) 428
SmlI CTYRAG 2 cut(s) 1095, 2394
SmoI CTYRAG 2 cut(s) 1095, 2394
SphI GCATGC 1 cut(s) 1962
SsiI CCGC 7 cut(s) 299, 620, 788, 1430, 1963, 2019, 2333
SspI AATATT 1 cut(s) 472
SspMI CTAG 3 cut(s) 615, 1200, 1710
SstI GAGCTC 2 cut(s) 156, 539
StyI CCWWGG 7 cut(s) 45, 900, 946, 1339, 1369, 1411, 1480
TaaI ACNGT 4 cut(s) 148, 337, 871, 2373
TaiI ACGT 1 cut(s) 1105
TaqI TCGA 1 cut(s) 137
TauI GCSGC 4 cut(s) 623, 790, 1965, 2335
TfiI GAWTC 6 cut(s) 829, 1519, 1861, 2227, 2248, 2269
Tru1I TTAA 7 cut(s) 72, 668, 704, 1124, 1293, 2004, 2485
Tru9I TTAA 7 cut(s) 72, 668, 704, 1124, 1293, 2004, 2485
TscAI CASTG 3 cut(s) 435, 816, 1914
TseFI GTSAC 6 cut(s) 163, 972, 1048, 1347, 1563, 2445
Tsp45I GTSAC 6 cut(s) 163, 972, 1048, 1347, 1563, 2445
TspDTI ATGAA 8 cut(s) 480, 842, 1124, 1233, 1287, 1472, 1823, 1874
TspGWI ACGGA 2 cut(s) 223, 923
TspRI CASTG 3 cut(s) 435, 816, 1914
Tth111I GACNNNGTC 1 cut(s) 970
VpaK11BI GGWCC 6 cut(s) 50, 818, 905, 1254, 1427, 1801
XagI CCTNNNNNAGG 2 cut(s) 945, 1527
XapI RAATTY 5 cut(s) 444, 451, 679, 1669, 2064
XceI RCATGY 3 cut(s) 1184, 1962, 2095
XcmI CCANNNNNNNNNTGG 1 cut(s) 898
XspI CTAG 3 cut(s) 615, 1200, 1710
Zsp2I ATGCAT 1 cut(s) 1182
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.