pycom16g05330

Purple acid phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0002060
Physical Location & Seq
Reverse (-)
3454610 .. 3456188
1579 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 801 bp
ATGCATTCCCCAGCTGCGACACTAACTTTTGATCGTCATAGCTTGTGTGGTGCACCAGCAAGAACAGTGGGATGGCGTGACCCTGGATTCATACATACTAGTTTTCTGAAGGAGTTGTGGCCAAACAGACAGTACACGTACAAGCTGGGACATAGATTGTTTAATGGTACATACATTTGGAGTCGAACGTATCAGTTTAGAGCATCTCCTTATCCTGGTCAAAATTCTTTACAGCGCATAGTCATTTTTGGTGACATGGGAAAGGATGAAGCTGATGGTTCCAATGAATACAACAATTTCCAACCAGGTTCGCTTAACACTACTAAGCAACTTATTCGTGACTTGAAAAACATCGATATAGTCTTCCACATTGGGGATATATGTTACGCGAATGGTTATCTTTCACAATGGGACCAGTTTACTGCACAGGTCGAACCAATAGCATCAACTGTTCCTTACATGATTGCAAGTGGAAACCATGAACGAGACTGGCCAGGAACAGGTTCTTTCTATCAGAACATGGATTCAGGAGGCGAATGTGGTGTGTTGGCTGAAAATATGTTCTATGTTCCCACAGAGAACAGAGCTAAGTTTTGGTACAAAACTGACTATGGCATGTTTCGCTTCTGCATAGCAGACACGGAACATGACTGGAGAGAGGGCATGGAGCAGTACAAGTTCATCGAGCACTGCTTAGCATCAGTCGATAGGCAGAAGCAACCCTGGCTAATATTTCTTGCACATCGAGTTCTGGGATACTCTTCAGATTTCTATTACGCGGAAGAAGGATCGATTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Metallophos Metallophos_C Pur_ac_phosph_N

Protein Analysis

267

Amino Acids

30.77

Weight (kDa)

5.83

Isoelectric Point (pI)

39.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Metallophos PF00149 79 - 257 6.1e-13 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 389, 779
AciI CCGC 1 cut(s) 779
AclWI GGATC 1 cut(s) 796
AcoI YGGCCR 2 cut(s) 119, 491
AcsI RAATTY 1 cut(s) 223
AcuI CTGAAG 2 cut(s) 128, 747
AfaI GTAC 5 cut(s) 134, 140, 169, 599, 674
AfiI CCNNNNNNNGG 3 cut(s) 215, 373, 500
AflIII ACRYGT 1 cut(s) 135
AgsI TTSAA 1 cut(s) 346
AhlI ACTAGT 1 cut(s) 98
AjnI CCWGG 5 cut(s) 82, 214, 304, 493, 722
AluBI AGCT 5 cut(s) 14, 42, 145, 272, 587
AluI AGCT 5 cut(s) 14, 42, 145, 272, 587
Alw21I GWGCWC 2 cut(s) 55, 690
Alw26I GTCTC 1 cut(s) 480
Alw44I GTGCAC 1 cut(s) 51
AlwI GGATC 1 cut(s) 796
AoxI GGCC 2 cut(s) 119, 491
ApaLI GTGCAC 1 cut(s) 51
ApeKI GCWGC 1 cut(s) 14
ApoI RAATTY 1 cut(s) 223
Asp700I GAANNNNTTC 1 cut(s) 502
AspLEI GCGC 1 cut(s) 237
AspS9I GGNCC 1 cut(s) 412
AsuHPI GGTGA 1 cut(s) 263
AvaII GGWCC 1 cut(s) 412
BaeGI GKGCMC 1 cut(s) 55
BalI TGGCCA 2 cut(s) 121, 493
BbsI GAAGAC 1 cut(s) 355
Bbv12I GWGCWC 2 cut(s) 55, 690
BccI CCATC 2 cut(s) 66, 269
BcgI CGANNNNNNTGC 2 cut(s) 317, 351
BciT130I CCWGG 5 cut(s) 84, 216, 306, 495, 724
BciVI GTATCC 1 cut(s) 749
BcoDI GTCTC 1 cut(s) 480
BcuI ACTAGT 1 cut(s) 98
BfaI CTAG 1 cut(s) 99
BfuI GTATCC 1 cut(s) 749
BisI GCNGC 1 cut(s) 15
BlpI GCTNAGC 1 cut(s) 694
BlsI GCNGC 1 cut(s) 16
Bme1390I CCNGG 5 cut(s) 84, 216, 306, 495, 724
Bme18I GGWCC 1 cut(s) 412
BmgT120I GGNCC 1 cut(s) 412
BmiI GGNNCC 2 cut(s) 280, 413
BmrFI CCNGG 5 cut(s) 84, 216, 306, 495, 724
BmsI GCATC 3 cut(s) 212, 452, 707
BpiI GAAGAC 1 cut(s) 355
BpmI CTGGAG 1 cut(s) 673
Bpu1102I GCTNAGC 1 cut(s) 694
Bsa29I ATCGAT 2 cut(s) 354, 791
BsaAI YACGTR 1 cut(s) 138
BsaJI CCNNGG 2 cut(s) 82, 722
Bsc4I CCNNNNNNNGG 3 cut(s) 215, 373, 500
Bse1I ACTGG 3 cut(s) 415, 494, 656
BseBI CCWGG 5 cut(s) 84, 216, 306, 495, 724
BseCI ATCGAT 2 cut(s) 354, 791
BseDI CCNNGG 2 cut(s) 82, 722
BseGI GGATG 2 cut(s) 77, 271
BseLI CCNNNNNNNGG 3 cut(s) 215, 373, 500
BseNI ACTGG 3 cut(s) 415, 494, 656
BseSI GKGCMC 1 cut(s) 55
BseYI CCCAGC 2 cut(s) 10, 145
BsgI GTGCAG 1 cut(s) 408
Bsh1236I CGCG 2 cut(s) 389, 779
BshFI GGCC 2 cut(s) 121, 493
BshVI ATCGAT 2 cut(s) 354, 791
BsiHKAI GWGCWC 2 cut(s) 55, 690
BslFI GGGAC 2 cut(s) 162, 425
BslI CCNNNNNNNGG 3 cut(s) 215, 373, 500
BsmAI GTCTC 1 cut(s) 480
BsmFI GGGAC 2 cut(s) 162, 425
BsmI GAATGC 1 cut(s) 4
BsnI GGCC 2 cut(s) 121, 493
Bsp1286I GDGCHC 2 cut(s) 55, 690
Bsp143I GATC 2 cut(s) 31, 788
Bsp1720I GCTNAGC 1 cut(s) 694
BspACI CCGC 1 cut(s) 779
BspANI GGCC 2 cut(s) 121, 493
BspDI ATCGAT 2 cut(s) 354, 791
BspFNI CGCG 2 cut(s) 389, 779
BspLI GGNNCC 2 cut(s) 280, 413
BspPI GGATC 1 cut(s) 796
BsrI ACTGG 3 cut(s) 415, 494, 656
BssECI CCNNGG 2 cut(s) 82, 722
BssMI GATC 2 cut(s) 31, 788
Bst2UI CCWGG 5 cut(s) 84, 216, 306, 495, 724
Bst4CI ACNGT 3 cut(s) 67, 132, 451
Bst6I CTCTTC 1 cut(s) 766
BstBAI YACGTR 1 cut(s) 138
BstDEI CTNAG 3 cut(s) 324, 588, 694
BstF5I GGATG 2 cut(s) 77, 271
BstFNI CGCG 2 cut(s) 389, 779
BstHHI GCGC 1 cut(s) 237
BstKTI GATC 2 cut(s) 34, 791
BstMAI GTCTC 1 cut(s) 480
BstMBI GATC 2 cut(s) 31, 788
BstMWI GCNNNNNNNGC 2 cut(s) 621, 724
BstNI CCWGG 5 cut(s) 84, 216, 306, 495, 724
BstNSI RCATGY 1 cut(s) 619
BstSCI CCNGG 5 cut(s) 82, 214, 304, 493, 722
BstSLI GKGCMC 1 cut(s) 55
BstUI CGCG 2 cut(s) 389, 779
BstV2I GAAGAC 1 cut(s) 355
Bsu15I ATCGAT 2 cut(s) 354, 791
BsuI GTATCC 1 cut(s) 749
BsuRI GGCC 2 cut(s) 121, 493
BsuTUI ATCGAT 2 cut(s) 354, 791
BtsCI GGATG 2 cut(s) 77, 271
BtsI GCAGTG 1 cut(s) 688
BtsIMutI CAGTG 2 cut(s) 72, 688
CfoI GCGC 1 cut(s) 237
Cfr13I GGNCC 1 cut(s) 412
ClaI ATCGAT 2 cut(s) 354, 791
CsiI ACCWGGT 1 cut(s) 304
Csp6I GTAC 5 cut(s) 133, 139, 168, 598, 673
CspCI CAANNNNNGTGG 2 cut(s) 48, 83
CviAII CATG 7 cut(s) 256, 460, 479, 520, 616, 647, 664
CviJI RGCY 9 cut(s) 14, 42, 121, 145, 272, 493, 551, 587, 727
CviKI_1 RGCY 9 cut(s) 14, 42, 121, 145, 272, 493, 551, 587, 727
CviQI GTAC 5 cut(s) 133, 139, 168, 598, 673
DdeI CTNAG 3 cut(s) 324, 588, 694
DpnI GATC 2 cut(s) 33, 790
DpnII GATC 2 cut(s) 31, 788
EaeI YGGCCR 2 cut(s) 119, 491
Eam1104I CTCTTC 1 cut(s) 766
EarI CTCTTC 1 cut(s) 766
Eco47I GGWCC 1 cut(s) 412
Eco57I CTGAAG 2 cut(s) 128, 747
EcoRII CCWGG 5 cut(s) 82, 214, 304, 493, 722
EcoT22I ATGCAT 1 cut(s) 6
FaeI CATG 7 cut(s) 259, 463, 482, 523, 619, 650, 667
FaqI GGGAC 2 cut(s) 162, 425
FatI CATG 7 cut(s) 255, 459, 478, 519, 615, 646, 663
Fnu4HI GCNGC 1 cut(s) 15
FokI GGATG 2 cut(s) 84, 278
Fsp4HI GCNGC 1 cut(s) 15
FspBI CTAG 1 cut(s) 99
GlaI GCGC 1 cut(s) 236
GluI GCNGC 1 cut(s) 15
GsaI CCCAGC 2 cut(s) 14, 149
GsuI CTGGAG 1 cut(s) 673
HaeIII GGCC 2 cut(s) 121, 493
HhaI GCGC 1 cut(s) 237
Hin1II CATG 7 cut(s) 259, 463, 482, 523, 619, 650, 667
Hin6I GCGC 1 cut(s) 235
HinP1I GCGC 1 cut(s) 235
HinfI GANTC 3 cut(s) 87, 181, 524
HphI GGTGA 1 cut(s) 263
Hpy166II GTNNAC 3 cut(s) 53, 135, 420
Hpy188I TCNGA 3 cut(s) 108, 516, 766
Hpy188III TCNNGA 2 cut(s) 338, 528
Hpy8I GTNNAC 3 cut(s) 53, 135, 420
HpyAV CCTTC 2 cut(s) 103, 779
HpyCH4III ACNGT 3 cut(s) 67, 132, 451
HpyCH4IV ACGT 2 cut(s) 137, 188
HpyCH4V TGCA 6 cut(s) 4, 53, 425, 467, 630, 740
HpyF10VI GCNNNNNNNGC 2 cut(s) 621, 724
HpyF3I CTNAG 3 cut(s) 324, 588, 694
HpySE526I ACGT 2 cut(s) 137, 188
Hsp92II CATG 7 cut(s) 259, 463, 482, 523, 619, 650, 667
HspAI GCGC 1 cut(s) 235
Kzo9I GATC 2 cut(s) 31, 788
LmnI GCTCC 1 cut(s) 667
LweI GCATC 3 cut(s) 212, 452, 707
MabI ACCWGGT 1 cut(s) 304
MaeI CTAG 1 cut(s) 99
MaeII ACGT 2 cut(s) 137, 188
MaeIII GTNAC 4 cut(s) 77, 251, 338, 383
MalI GATC 2 cut(s) 33, 790
MboI GATC 2 cut(s) 31, 788
MboII GAAGA 3 cut(s) 355, 753, 794
MhlI GDGCHC 2 cut(s) 55, 690
MlsI TGGCCA 2 cut(s) 121, 493
MluCI AATT 2 cut(s) 223, 295
MluNI TGGCCA 2 cut(s) 121, 493
MlyI GAGTC 1 cut(s) 190
MmeI TCCRAC 1 cut(s) 325
MnlI CCTC 2 cut(s) 524, 652
Mox20I TGGCCA 2 cut(s) 121, 493
Mph1103I ATGCAT 1 cut(s) 6
MroXI GAANNNNTTC 1 cut(s) 502
MscI TGGCCA 2 cut(s) 121, 493
MseI TTAA 3 cut(s) 162, 315, 799
Msp20I TGGCCA 2 cut(s) 121, 493
MspA1I CMGCKG 1 cut(s) 14
MspR9I CCNGG 5 cut(s) 84, 216, 306, 495, 724
Mva1269I GAATGC 1 cut(s) 4
MvaI CCWGG 5 cut(s) 84, 216, 306, 495, 724
MvnI CGCG 2 cut(s) 389, 779
MwoI GCNNNNNNNGC 2 cut(s) 621, 724
NdeII GATC 2 cut(s) 31, 788
NlaIII CATG 7 cut(s) 259, 463, 482, 523, 619, 650, 667
NlaIV GGNNCC 2 cut(s) 280, 413
NmuCI GTSAC 3 cut(s) 77, 251, 338
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 1 cut(s) 619
PctI GAATGC 1 cut(s) 4
PdmI GAANNNNTTC 1 cut(s) 502
PfeI GAWTC 2 cut(s) 87, 524
PkrI GCNGC 1 cut(s) 16
PleI GAGTC 1 cut(s) 189
PpsI GAGTC 1 cut(s) 189
Ppu21I YACGTR 1 cut(s) 138
Psp6I CCWGG 5 cut(s) 82, 214, 304, 493, 722
PspFI CCCAGC 2 cut(s) 10, 145
PspGI CCWGG 5 cut(s) 82, 214, 304, 493, 722
PspN4I GGNNCC 2 cut(s) 280, 413
PspPI GGNCC 1 cut(s) 412
PvuII CAGCTG 1 cut(s) 14
RsaI GTAC 5 cut(s) 134, 140, 169, 599, 674
RsaNI GTAC 5 cut(s) 133, 139, 168, 598, 673
SaqAI TTAA 3 cut(s) 162, 315, 799
SatI GCNGC 1 cut(s) 15
Sau3AI GATC 2 cut(s) 31, 788
Sau96I GGNCC 1 cut(s) 412
SchI GAGTC 1 cut(s) 190
ScrFI CCNGG 5 cut(s) 84, 216, 306, 495, 724
SduI GDGCHC 2 cut(s) 55, 690
SexAI ACCWGGT 1 cut(s) 304
SfaNI GCATC 3 cut(s) 212, 452, 707
SinI GGWCC 1 cut(s) 412
SpeI ACTAGT 1 cut(s) 98
Sse9I AATT 2 cut(s) 223, 295
SsiI CCGC 1 cut(s) 779
SspI AATATT 1 cut(s) 732
SspMI CTAG 1 cut(s) 99
StyD4I CCNGG 5 cut(s) 82, 214, 304, 493, 722
TaaI ACNGT 3 cut(s) 67, 132, 451
TaiI ACGT 2 cut(s) 140, 191
TaqI TCGA 7 cut(s) 184, 354, 432, 684, 705, 745, 791
TasI AATT 2 cut(s) 223, 295
TatI WGTACW 2 cut(s) 132, 672
TfiI GAWTC 2 cut(s) 87, 524
Tru1I TTAA 3 cut(s) 162, 315, 799
Tru9I TTAA 3 cut(s) 162, 315, 799
TscAI CASTG 2 cut(s) 72, 695
TseFI GTSAC 3 cut(s) 77, 251, 338
TseI GCWGC 1 cut(s) 14
Tsp45I GTSAC 3 cut(s) 77, 251, 338
TspDTI ATGAA 5 cut(s) 79, 282, 300, 495, 670
TspGWI ACGGA 1 cut(s) 656
TspRI CASTG 2 cut(s) 72, 695
VneI GTGCAC 1 cut(s) 51
VpaK11BI GGWCC 1 cut(s) 412
XapI RAATTY 1 cut(s) 223
XceI RCATGY 1 cut(s) 619
XmnI GAANNNNTTC 1 cut(s) 502
XspI CTAG 1 cut(s) 99
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.