pycom16g05340

Purple acid phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0002060
Physical Location & Seq
Reverse (-)
3459872 .. 3463652
3781 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2034 bp
ATGGGAGCAAAAGTCGAATCTTCCCTTAAGATTCCCAAAGTTGTTAACGATTTGCAATCATTGAAAACTCAGAAACCCAAAAAAATCTTGAACTTCAGTTCGACTGCATCACTCAAAGTTTGCAGCTTTCTTCCTTTGTTCTCACAAAGATTCTTCATTTTTCATGCCGTGTGGGATATTGGGATGAAAGAAATGAAACCAGTGAGGCTGTTGGCAATTCTGTTGGTTGTTGTTGTTGCAACCCTTCAAGTTGAGAAGGCAAGCTCGCATGGAGATCAACCTCTTTCCAGAATTGCTATTCATAGAGCAACCTTTGCACTTGAAGAGCCTGCTTACATTCAAGCCTCTCCTTCTGTTCTTGGATTAAGGGGGCAAAACGCAGAGTGGGTGAAGTTGGAATTTGGTACCGAAAACCCGAAAATCGATGATTGGATTGGAGTGTTTTCTCCTGCTAATTTCAGTGCTTCCACCTGCCCTGAGGAGAATCCGAGAGTTTTTCCTCCGTTTTTGTGTTCTGCGCCGATTAAGTTTCAGTATGCGAATTACTCCACTCCCGGCTACAAAGACACTGGAAAAGGGTTCTTGAAGCTTCAGCTCATCAACCAGAGATCGGAGTTCTCTTTCGCACTATTTTCTGGCGGTTTATTGAATCCAAAGGTGGTGGCGGTGTCAAATCATGTAGCTTTCGCCAATCCAGATGCACCAGTTTACCCGCGCATAGCACAAGGAAAAGAATGGAATGAAATGACCGTAACATGGACAAGTGGATATGGGCTCAATGACGCAGAACCTTTTGTTGAATGGGGTCCGAGAGGAGAAACTACGCGTTCCCCTGCTGTGACGCTAACTTTTGATCGTCGTAGCTTGTGTGGTGCACCAGCAAGAACAGTGGGATGGCGTGACCCTGGATTCATACATACTGGTTTTCTGAAGGAGTTGTGGCCAAACAGACAGTACACGTACAAGCTGGGACATAGATTGTTTAATGGTACATACATTTGGAGTCGAACGTATCAGTTTAGAGCATCCCCTTATCCTGGTCAAAATTCTTTACAGCGTGTAGTCATTTTCGGTGACATGGGAAAGGATGAAGCTGATGGTTCCAATGAATACAACAACTTCCAGCCAGGTTCGCTTAACACTACTAAGCAACTTATTCGAGACTTGAAAAACATCGATATAGTCTTCCACATTGGGGATATATGTTATGCAAATGGTTATCTTTCACAATGGGACCAATTTACTGCACAGGTCGAACCAATAGCATCAACTGTTCCTTACATGATTGCTAGTGGAAACCATGAACGAGACTGGCCAGGAACGGGTTCTTTCTATAAGAACATGGATTCAGGAGGTGAATGCGGTGTGTTGGCTGAAAATATGTTCTATGTTCCCACGGAGAATAGAGCTAAGTTTTGGTACAAGACGGACTACGGCATGTTTCGCTTCTGCATAGCAGACACGGAACATGACTGGAGAGAGGGCACAGAGCAGTACAAGTTCATCGAGCACTGCTTAGCATCAGTTGATAGGCAGAAGCAACCCTGGCTAATATTTCTCGCACATCGAGTTCTAGGATACTCTTCGGATGCCTCTTATGCGGTAGAAGGATCATTTGCAGAACCAATGGGGAGGGAGAGCCTCCAAAAACTCTGGCAAAAGTATAAGGTCGACATTGGCATCTACGGCCATGTCCATAACTACGAAAGAACCTGTCCCATATACCAGAATATTTGCACCAATGAAGAGAGACACCATTATAAGGGCAGCTTGAATGGAACAATACATGTGGCTGCTGGTGGTGCGGGAGCAAGCCTGTCACCGTTTACTTCCCTCCAAACGAAATGGAGTGTTTTCAAAGACTACGACCATGGATTCGTCAAGCTCACAGCATTCGATCGCTCAAACCTTTTGTTCGAGTACAAGAAGAGCAGGGACGGGCAGGTTTACGACTCGTTCAGAATATCCAGAGATTATAGGGACATCTTGGCCTGCGCCGTTGATAGCTGCCCTAGCACCACACTTGCCTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Metallophos Metallophos_C Pur_ac_phosph_N

Protein Analysis

678

Amino Acids

76.52

Weight (kDa)

8.27

Isoelectric Point (pI)

36.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
fn3_PAP PF17808 113 - 231 1e-40 Fn3-like domain from Purple Acid Phosphatase
Pur_ac_phosph_N PF16656 239 - 341 4.7e-11 Purple acid Phosphatase, N-terminal domain
Metallophos PF00149 353 - 568 2.7e-17 Calcineurin-like phosphoesterase
Metallophos_C PF14008 593 - 652 1.1e-14 Iron/zinc purple acid phosphatase-like protein C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1761
AarI CACCTGC 1 cut(s) 479
Acc36I ACCTGC 2 cut(s) 479, 1933
Acc65I GGTACC 1 cut(s) 404
AccB1I GGYRCC 1 cut(s) 404
AccI GTMKAC 1 cut(s) 1671
AccII CGCG 2 cut(s) 715, 826
AciI CCGC 6 cut(s) 639, 665, 713, 1362, 1601, 1805
AclWI GGATC 1 cut(s) 1618
AcoI YGGCCR 3 cut(s) 941, 1313, 1687
AcsI RAATTY 2 cut(s) 398, 1045
AcuI CTGAAG 3 cut(s) 79, 575, 950
AfaI GTAC 7 cut(s) 406, 956, 962, 991, 1421, 1496, 1922
AfiI CCNNNNNNNGG 6 cut(s) 610, 756, 1037, 1195, 1322, 1762
AflII CTTAAG 1 cut(s) 26
AflIII ACRYGT 3 cut(s) 824, 957, 1786
AjnI CCWGG 5 cut(s) 904, 1036, 1126, 1315, 1544
Alw21I GWGCWC 2 cut(s) 877, 1512
Alw26I GTCTC 3 cut(s) 1155, 1302, 1744
Alw44I GTGCAC 1 cut(s) 873
AlwI GGATC 1 cut(s) 1618
AoxI GGCC 4 cut(s) 941, 1313, 1687, 1989
ApaLI GTGCAC 1 cut(s) 873
ApeKI GCWGC 4 cut(s) 123, 1767, 1793, 2007
ApoI RAATTY 2 cut(s) 398, 1045
ArsI GACNNNNNNTTYG 2 cut(s) 1859, 1891
Asp700I GAANNNNTTC 1 cut(s) 1324
Asp718I GGTACC 1 cut(s) 404
AspLEI GCGC 3 cut(s) 520, 717, 1997
AspS9I GGNCC 2 cut(s) 806, 1234
AsuC2I CCSGG 1 cut(s) 555
AsuHPI GGTGA 4 cut(s) 400, 1085, 1367, 1812
AvaII GGWCC 2 cut(s) 806, 1234
AxyI CCTNAGG 1 cut(s) 477
BaeGI GKGCMC 2 cut(s) 877, 1487
BalI TGGCCA 2 cut(s) 943, 1315
BanI GGYRCC 1 cut(s) 404
BanII GRGCYC 1 cut(s) 777
BbsI GAAGAC 1 cut(s) 1177
Bbv12I GWGCWC 2 cut(s) 877, 1512
BbvI GCAGC 4 cut(s) 135, 1779, 1780, 1994
BccI CCATC 2 cut(s) 888, 1091
BceAI ACGGC 4 cut(s) 152, 1450, 1702, 1982
BcgI CGANNNNNNTGC 2 cut(s) 1139, 1173
BciT130I CCWGG 5 cut(s) 906, 1038, 1128, 1317, 1546
BciVI GTATCC 1 cut(s) 1571
BcnI CCSGG 1 cut(s) 555
BcoDI GTCTC 3 cut(s) 1155, 1302, 1744
BfaI CTAG 3 cut(s) 1290, 1574, 2013
BfrI CTTAAG 1 cut(s) 26
BfuAI ACCTGC 2 cut(s) 479, 1933
BfuI GTATCC 1 cut(s) 1571
BisI GCNGC 4 cut(s) 124, 1768, 1794, 2008
BlpI GCTNAGC 1 cut(s) 1516
BlsI GCNGC 4 cut(s) 125, 1769, 1795, 2009
Bme1390I CCNGG 6 cut(s) 555, 906, 1038, 1128, 1317, 1546
Bme18I GGWCC 2 cut(s) 806, 1234
BmgT120I GGNCC 2 cut(s) 806, 1234
BmiI GGNNCC 4 cut(s) 406, 807, 1102, 1235
BmrFI CCNGG 6 cut(s) 555, 906, 1038, 1128, 1317, 1546
BmsI GCATC 7 cut(s) 116, 688, 1034, 1274, 1529, 1579, 1689
BpiI GAAGAC 1 cut(s) 1177
BpmI CTGGAG 1 cut(s) 1495
Bpu1102I GCTNAGC 1 cut(s) 1516
BpuMI CCSGG 1 cut(s) 555
Bsa29I ATCGAT 2 cut(s) 423, 1176
BsaAI YACGTR 1 cut(s) 960
BsaJI CCNNGG 4 cut(s) 904, 1395, 1544, 1870
BsaXI ACNNNNNCTCC 1 cut(s) 27
Bsc4I CCNNNNNNNGG 6 cut(s) 610, 756, 1037, 1195, 1322, 1762
Bse1I ACTGG 6 cut(s) 200, 574, 704, 925, 1316, 1478
Bse21I CCTNAGG 1 cut(s) 477
BseBI CCWGG 5 cut(s) 906, 1038, 1128, 1317, 1546
BseCI ATCGAT 2 cut(s) 423, 1176
BseDI CCNNGG 4 cut(s) 904, 1395, 1544, 1870
BseGI GGATG 5 cut(s) 189, 899, 1025, 1093, 1594
BseLI CCNNNNNNNGG 6 cut(s) 610, 756, 1037, 1195, 1322, 1762
BseMII CTCAG 2 cut(s) 83, 468
BseNI ACTGG 6 cut(s) 200, 574, 704, 925, 1316, 1478
BseRI GAGGAG 2 cut(s) 494, 828
BseSI GKGCMC 2 cut(s) 877, 1487
BseXI GCAGC 4 cut(s) 135, 1779, 1780, 1994
BseYI CCCAGC 1 cut(s) 967
BsgI GTGCAG 1 cut(s) 1230
Bsh1236I CGCG 2 cut(s) 715, 826
Bsh1285I CGRYCG 1 cut(s) 1900
BshFI GGCC 4 cut(s) 943, 1315, 1689, 1991
BshNI GGYRCC 1 cut(s) 404
BshVI ATCGAT 2 cut(s) 423, 1176
BsiEI CGRYCG 1 cut(s) 1900
BsiHKAI GWGCWC 2 cut(s) 877, 1512
BsiSI CCGG 1 cut(s) 555
BslFI GGGAC 5 cut(s) 984, 1247, 1701, 1949, 1994
BslI CCNNNNNNNGG 6 cut(s) 610, 756, 1037, 1195, 1322, 1762
BsmAI GTCTC 3 cut(s) 1155, 1302, 1744
BsmFI GGGAC 5 cut(s) 984, 1247, 1701, 1949, 1994
BsmI GAATGC 2 cut(s) 1364, 1892
BsnI GGCC 4 cut(s) 943, 1315, 1689, 1991
Bsp1286I GDGCHC 4 cut(s) 777, 877, 1487, 1512
Bsp143I GATC 5 cut(s) 274, 608, 853, 1610, 1897
Bsp1720I GCTNAGC 1 cut(s) 1516
Bsp19I CCATGG 1 cut(s) 1870
BspACI CCGC 6 cut(s) 639, 665, 713, 1362, 1601, 1805
BspANI GGCC 4 cut(s) 943, 1315, 1689, 1991
BspCNI CTCAG 2 cut(s) 82, 469
BspDI ATCGAT 2 cut(s) 423, 1176
BspFNI CGCG 2 cut(s) 715, 826
BspHI TCATGA 1 cut(s) 2030
BspLI GGNNCC 4 cut(s) 406, 807, 1102, 1235
BspMI ACCTGC 2 cut(s) 479, 1933
BspPI GGATC 1 cut(s) 1618
BspQI GCTCTTC 2 cut(s) 318, 1922
BspT107I GGYRCC 1 cut(s) 404
BspTI CTTAAG 1 cut(s) 26
BsrI ACTGG 6 cut(s) 200, 574, 704, 925, 1316, 1478
BssECI CCNNGG 4 cut(s) 904, 1395, 1544, 1870
BssMI GATC 5 cut(s) 274, 608, 853, 1610, 1897
BssT1I CCWWGG 1 cut(s) 1870
Bst2UI CCWGG 5 cut(s) 906, 1038, 1128, 1317, 1546
Bst4CI ACNGT 5 cut(s) 751, 889, 954, 1273, 1824
Bst6I CTCTTC 4 cut(s) 318, 1588, 1740, 1922
BstAFI CTTAAG 1 cut(s) 26
BstAPI GCANNNNNTGC 1 cut(s) 314
BstBAI YACGTR 1 cut(s) 960
BstC8I GCNNGC 5 cut(s) 262, 266, 330, 1813, 1993
BstDEI CTNAG 5 cut(s) 69, 477, 1146, 1410, 1516
BstDSI CCRYGG 2 cut(s) 1395, 1870
BstF5I GGATG 5 cut(s) 189, 899, 1025, 1093, 1594
BstFNI CGCG 2 cut(s) 715, 826
BstHHI GCGC 3 cut(s) 520, 717, 1997
BstKTI GATC 5 cut(s) 277, 611, 856, 1613, 1900
BstMAI GTCTC 3 cut(s) 1155, 1302, 1744
BstMBI GATC 5 cut(s) 274, 608, 853, 1610, 1897
BstMCI CGRYCG 1 cut(s) 1900
BstMWI GCNNNNNNNGC 8 cut(s) 314, 1132, 1443, 1546, 1598, 1686, 1802, 2013
BstNI CCWGG 5 cut(s) 906, 1038, 1128, 1317, 1546
BstNSI RCATGY 2 cut(s) 1441, 1790
BstSCI CCNGG 6 cut(s) 553, 904, 1036, 1126, 1315, 1544
BstSLI GKGCMC 2 cut(s) 877, 1487
BstUI CGCG 2 cut(s) 715, 826
BstV1I GCAGC 4 cut(s) 135, 1779, 1780, 1994
BstV2I GAAGAC 1 cut(s) 1177
Bsu15I ATCGAT 2 cut(s) 423, 1176
Bsu36I CCTNAGG 1 cut(s) 477
BsuI GTATCC 1 cut(s) 1571
BsuRI GGCC 4 cut(s) 943, 1315, 1689, 1991
BsuTUI ATCGAT 2 cut(s) 423, 1176
BtgI CCRYGG 2 cut(s) 1395, 1870
BtsCI GGATG 5 cut(s) 189, 899, 1025, 1093, 1594
BtsI GCAGTG 1 cut(s) 1510
BtsIMutI CAGTG 5 cut(s) 207, 466, 567, 894, 1510
BveI ACCTGC 2 cut(s) 479, 1933
Cac8I GCNNGC 5 cut(s) 262, 266, 330, 1813, 1993
CciI TCATGA 1 cut(s) 2030
CfoI GCGC 3 cut(s) 520, 717, 1997
Cfr13I GGNCC 2 cut(s) 806, 1234
ClaI ATCGAT 2 cut(s) 423, 1176
CseI GACGC 2 cut(s) 791, 850
Csp6I GTAC 7 cut(s) 405, 955, 961, 990, 1420, 1495, 1921
CspCI CAANNNNNGTGG 6 cut(s) 642, 677, 870, 905, 1770, 1805
CviQI GTAC 7 cut(s) 405, 955, 961, 990, 1420, 1495, 1921
DdeI CTNAG 5 cut(s) 69, 477, 1146, 1410, 1516
DpnI GATC 5 cut(s) 276, 610, 855, 1612, 1899
DpnII GATC 5 cut(s) 274, 608, 853, 1610, 1897
EaeI YGGCCR 3 cut(s) 941, 1313, 1687
Eam1104I CTCTTC 4 cut(s) 318, 1588, 1740, 1922
EarI CTCTTC 4 cut(s) 318, 1588, 1740, 1922
Eco130I CCWWGG 1 cut(s) 1870
Eco24I GRGCYC 1 cut(s) 777
Eco47I GGWCC 2 cut(s) 806, 1234
Eco57I CTGAAG 3 cut(s) 79, 575, 950
Eco81I CCTNAGG 1 cut(s) 477
EcoRII CCWGG 5 cut(s) 904, 1036, 1126, 1315, 1544
EcoT14I CCWWGG 1 cut(s) 1870
EcoT38I GRGCYC 1 cut(s) 777
ErhI CCWWGG 1 cut(s) 1870
FalI AAGNNNNNCTT 2 cut(s) 1754, 1786
FaqI GGGAC 5 cut(s) 984, 1247, 1701, 1949, 1994
FauI CCCGC 2 cut(s) 720, 1798
FblI GTMKAC 1 cut(s) 1671
Fnu4HI GCNGC 4 cut(s) 124, 1768, 1794, 2008
FokI GGATG 5 cut(s) 196, 906, 1012, 1100, 1601
FriOI GRGCYC 1 cut(s) 777
Fsp4HI GCNGC 4 cut(s) 124, 1768, 1794, 2008
FspBI CTAG 3 cut(s) 1290, 1574, 2013
GlaI GCGC 3 cut(s) 519, 716, 1996
GluI GCNGC 4 cut(s) 124, 1768, 1794, 2008
GsaI CCCAGC 1 cut(s) 971
GsuI CTGGAG 1 cut(s) 1495
HaeIII GGCC 4 cut(s) 943, 1315, 1689, 1991
HapII CCGG 1 cut(s) 555
HgaI GACGC 2 cut(s) 791, 850
HhaI GCGC 3 cut(s) 520, 717, 1997
Hin6I GCGC 3 cut(s) 518, 715, 1995
HinP1I GCGC 3 cut(s) 518, 715, 1995
HincII GTYRAC 2 cut(s) 46, 1672
HindII GTYRAC 2 cut(s) 46, 1672
HindIII AAGCTT 1 cut(s) 587
HpaI GTTAAC 1 cut(s) 46
HpaII CCGG 1 cut(s) 555
HphI GGTGA 4 cut(s) 400, 1085, 1367, 1812
Hpy166II GTNNAC 7 cut(s) 46, 709, 875, 957, 1672, 1827, 1948
Hpy188I TCNGA 7 cut(s) 72, 489, 613, 810, 930, 1588, 1961
Hpy188III TCNNGA 8 cut(s) 88, 288, 583, 695, 1160, 1350, 1968, 2031
Hpy8I GTNNAC 7 cut(s) 46, 709, 875, 957, 1672, 1827, 1948
Hpy99I CGWCG 1 cut(s) 861
HpyAV CCTTC 5 cut(s) 250, 254, 360, 925, 1601
HpyCH4III ACNGT 5 cut(s) 751, 889, 954, 1273, 1824
HpyCH4IV ACGT 2 cut(s) 959, 1010
HpyF10VI GCNNNNNNNGC 8 cut(s) 314, 1132, 1443, 1546, 1598, 1686, 1802, 2013
HpyF3I CTNAG 5 cut(s) 69, 477, 1146, 1410, 1516
HpySE526I ACGT 2 cut(s) 959, 1010
HspAI GCGC 3 cut(s) 518, 715, 1995
KpnI GGTACC 1 cut(s) 408
KspAI GTTAAC 1 cut(s) 46
Kzo9I GATC 5 cut(s) 274, 608, 853, 1610, 1897
LguI GCTCTTC 2 cut(s) 318, 1922
LmnI GCTCC 2 cut(s) 5, 1808
Lsp1109I GCAGC 4 cut(s) 135, 1779, 1780, 1994
LweI GCATC 7 cut(s) 116, 688, 1034, 1274, 1529, 1579, 1689
MaeI CTAG 3 cut(s) 1290, 1574, 2013
MaeII ACGT 2 cut(s) 959, 1010
MaeIII GTNAC 5 cut(s) 751, 838, 899, 1073, 1818
MalI GATC 5 cut(s) 276, 610, 855, 1612, 1899
MboI GATC 5 cut(s) 274, 608, 853, 1610, 1897
MboII GAAGA 8 cut(s) 12, 122, 145, 335, 1177, 1575, 1757, 1939
MhlI GDGCHC 4 cut(s) 777, 877, 1487, 1512
MlsI TGGCCA 2 cut(s) 943, 1315
MluCI AATT 7 cut(s) 216, 291, 398, 454, 541, 1045, 1238
MluI ACGCGT 1 cut(s) 824
MluNI TGGCCA 2 cut(s) 943, 1315
MlyI GAGTC 2 cut(s) 1012, 1946
MmeI TCCRAC 1 cut(s) 375
Mox20I TGGCCA 2 cut(s) 943, 1315
MroXI GAANNNNTTC 1 cut(s) 1324
MscI TGGCCA 2 cut(s) 943, 1315
MseI TTAA 6 cut(s) 27, 45, 365, 525, 984, 1137
Msp20I TGGCCA 2 cut(s) 943, 1315
MspCI CTTAAG 1 cut(s) 26
MspI CCGG 1 cut(s) 555
MspR9I CCNGG 6 cut(s) 555, 906, 1038, 1128, 1317, 1546
Mva1269I GAATGC 2 cut(s) 1364, 1892
MvaI CCWGG 5 cut(s) 906, 1038, 1128, 1317, 1546
MvnI CGCG 2 cut(s) 715, 826
MwoI GCNNNNNNNGC 8 cut(s) 314, 1132, 1443, 1546, 1598, 1686, 1802, 2013
NciI CCSGG 1 cut(s) 555
NcoI CCATGG 1 cut(s) 1870
NdeII GATC 5 cut(s) 274, 608, 853, 1610, 1897
NlaIV GGNNCC 4 cut(s) 406, 807, 1102, 1235
NmuCI GTSAC 4 cut(s) 838, 899, 1073, 1818
NspI RCATGY 2 cut(s) 1441, 1790
PagI TCATGA 1 cut(s) 2030
PaqCI CACCTGC 1 cut(s) 479
PciI ACATGT 1 cut(s) 1786
PciSI GCTCTTC 2 cut(s) 318, 1922
PctI GAATGC 2 cut(s) 1364, 1892
PdmI GAANNNNTTC 1 cut(s) 1324
PfeI GAWTC 8 cut(s) 17, 31, 150, 484, 649, 909, 1346, 1875
PkrI GCNGC 4 cut(s) 125, 1769, 1795, 2009
Ple19I CGATCG 1 cut(s) 1900
PleI GAGTC 2 cut(s) 1011, 1946
PpsI GAGTC 2 cut(s) 1011, 1946
Ppu21I YACGTR 1 cut(s) 960
PscI ACATGT 1 cut(s) 1786
PsiI TTATAA 1 cut(s) 1761
Psp6I CCWGG 5 cut(s) 904, 1036, 1126, 1315, 1544
PspFI CCCAGC 1 cut(s) 967
PspGI CCWGG 5 cut(s) 904, 1036, 1126, 1315, 1544
PspN4I GGNNCC 4 cut(s) 406, 807, 1102, 1235
PspPI GGNCC 2 cut(s) 806, 1234
PvuI CGATCG 1 cut(s) 1900
RsaI GTAC 7 cut(s) 406, 956, 962, 991, 1421, 1496, 1922
RsaNI GTAC 7 cut(s) 405, 955, 961, 990, 1420, 1495, 1921
SalI GTCGAC 1 cut(s) 1670
SapI GCTCTTC 2 cut(s) 318, 1922
SaqAI TTAA 6 cut(s) 27, 45, 365, 525, 984, 1137
SatI GCNGC 4 cut(s) 124, 1768, 1794, 2008
Sau3AI GATC 5 cut(s) 274, 608, 853, 1610, 1897
Sau96I GGNCC 2 cut(s) 806, 1234
SchI GAGTC 2 cut(s) 1012, 1946
ScrFI CCNGG 6 cut(s) 555, 906, 1038, 1128, 1317, 1546
SduI GDGCHC 4 cut(s) 777, 877, 1487, 1512
SfaNI GCATC 7 cut(s) 116, 688, 1034, 1274, 1529, 1579, 1689
SinI GGWCC 2 cut(s) 806, 1234
SmlI CTYRAG 1 cut(s) 26
SmoI CTYRAG 1 cut(s) 26
Sse9I AATT 7 cut(s) 216, 291, 398, 454, 541, 1045, 1238
SsiI CCGC 6 cut(s) 639, 665, 713, 1362, 1601, 1805
SspI AATATT 2 cut(s) 1554, 1732
SspMI CTAG 3 cut(s) 1290, 1574, 2013
StyD4I CCNGG 6 cut(s) 553, 904, 1036, 1126, 1315, 1544
StyI CCWWGG 1 cut(s) 1870
TaaI ACNGT 5 cut(s) 751, 889, 954, 1273, 1824
TaiI ACGT 2 cut(s) 962, 1013
TasI AATT 7 cut(s) 216, 291, 398, 454, 541, 1045, 1238
TatI WGTACW 3 cut(s) 954, 1494, 1920
TfiI GAWTC 8 cut(s) 17, 31, 150, 484, 649, 909, 1346, 1875
Tru1I TTAA 6 cut(s) 27, 45, 365, 525, 984, 1137
Tru9I TTAA 6 cut(s) 27, 45, 365, 525, 984, 1137
TscAI CASTG 5 cut(s) 207, 466, 574, 894, 1517
TseFI GTSAC 4 cut(s) 838, 899, 1073, 1818
TseI GCWGC 4 cut(s) 123, 1767, 1793, 2007
Tsp45I GTSAC 4 cut(s) 838, 899, 1073, 1818
TspGWI ACGGA 4 cut(s) 492, 1412, 1442, 1478
TspRI CASTG 5 cut(s) 207, 466, 574, 894, 1517
Vha464I CTTAAG 1 cut(s) 26
VneI GTGCAC 1 cut(s) 873
VpaK11BI GGWCC 2 cut(s) 806, 1234
XapI RAATTY 2 cut(s) 398, 1045
XceI RCATGY 2 cut(s) 1441, 1790
XmiI GTMKAC 1 cut(s) 1671
XmnI GAANNNNTTC 1 cut(s) 1324
XspI CTAG 3 cut(s) 1290, 1574, 2013
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.