Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr16

Orthogroup ID

OG0012815

Physical Location & Seq

Genomic Coordinates Forward (+)

5880574 .. 5882121

Gene Structure 1548 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1548 bp

ATGTATCGAAGTCTTGGCGTTCACCGCCTCATATACCGGAGCCGCATAGCTTACTACGTGAAAACTGGCCTCATCGATCAAGCACTCCAGGTGTTCGACGAAATGACCCACTCGGACTGCCGCGTTTTCAGCGTCGACTACAACCGCTTCATCGGCGTACTCGTCAAGCACTCGCGTTACGATCTCGCCGAGCACTACTACTACGAAATGGTGCCAAAGGGATTCTCCTTGAACCCGTTTACTTACTCCAGGTTCATTTCTGGCCTGTGTAAGATTAGAAATTTCACCCTCATTGAGAAGCTTCTTCGAGACATGGATAGGATTGGGGCATTGCCTGACATCTGGGCTTTCAATATATATTTGAATCTTCTGTGCCAAGAAAACAGGACGGATTTTGCTTTGGAATTGTTTCATAGGATGGTTGATAAAGGAAGAGAGCCGGATGTTGTATCGTACACTATAATTATCGATGGGTTGTGTAAAGCTGGACAATTTGATATGGCAGTTGATATTTGGAATGGTATGATTAGGAAAGGGTTTAGGCCGGATAACATTGCGTGCACGGCCCTGGTTGTTGGGTTGTGCAAGGGTGGGAAGGTTGATTTGGCTTATGACCTTGTCATTGATGAAATGAAGGGTAGAGTCAATTTTAGTAGTTTGATTTATAATGCTTTGATTAGCGGGTTTTGTCGTGCCGGTAGGATTGATAAGGCACAGGCAATCAAGTCATTTATGAAGAGAAATGGGTGCGAGCCGGATTTGGTTTCTCACAATGTGTTGTTGAATTATTGCTGTAATGCGTTCATGTTGGAGGAGGCTGAGAAGTTGATGAAGAAAATGGAGAGGGGTGGGATGGAACCGGATGTGTATAGTTATAATCAGCTTCTCATGGGCCTTTGTAAAGCTAATAGACCAGATAAGGCGTATTTGTTGATGCGAACTAGGATGGAGCCAAAAGGGTTGTGTAATGTTGTGTCATATAATACCATCATAACAGCATTTTGCAAGGCAAACCGAACTGGAAGTGCTTACAAACTGTTTGAGGAAATGCGTAACAAGGGCACTGTGCCAGATTTGGTTACATTCACTATTCTTATAAATGCCTTTTTAAGAGAAGGTAGCTCAGATATAGTCAAGAAGCTTCTTGATCAGATGACAGCAATGGGTCTGTTGCCTGACCGTATATTTTACACCACAATAGTTGATCACTTATGTAAGAGTGGGAATATTGAAATGGCTTATAGTGTTTTCAGTGAAATGCTAGAGAAGGGAATCACCCCTGATGTGATTTCATATAATGCTCTCATTAACGGGCTGCTCAAGGCTTCTAAAGTGAGCGACGCTATCCATCTTTATGAGGAAATGCAGACTAGAGGATGTAGCGCTGATGAGGTAACTTTTAAATTGATAATTGGGGGTCTCATAAGGGAGAATAAGGTTTCAGTCGCTTGTAGGGTATGGGATCAGATGATGGAAAAGGGCTTTACTCTTGATGGAGCTTTCTCTGAGACTCTGGTTAATGCCATCAATTCAAAAGATGCTGCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016020 GO:0016070 GO:0016787 GO:0016788 GO:0031090 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:0098588 GO:0098805 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2 PPR_3

Protein Analysis

516

Amino Acids

58.48

Weight (kDa)

8.2

Isoelectric Point (pI)

23.65

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	47 - 91	3.7e-07	PPR repeat family
PPR_2	PF13041	112 - 161	3.4e-15	PPR repeat family
PPR_1	PF12854	143 - 175	6e-10	PPR repeat
PPR_2	PF13041	147 - 196	1.1e-14	PPR repeat family
PPR	PF01535	150 - 180	4.1e-08	PPR repeat
PPR_3	PF13812	215 - 266	1.8e-07	Pentatricopeptide repeat domain
PPR_2	PF13041	220 - 266	1.8e-13	PPR repeat family
PPR	PF01535	221 - 250	1.7e-06	PPR repeat
PPR_1	PF12854	221 - 245	1.5e-07	PPR repeat
PPR_1	PF12854	248 - 281	3.1e-06	PPR repeat
PPR_2	PF13041	252 - 301	5.9e-14	PPR repeat family
PPR_1	PF12854	284 - 313	9.4e-08	PPR repeat
PPR_1	PF12854	320 - 351	4.8e-11	PPR repeat
PPR_3	PF13812	322 - 369	3.1e-07	Pentatricopeptide repeat domain
PPR_2	PF13041	323 - 370	5.4e-18	PPR repeat family
PPR	PF01535	325 - 354	1.4e-07	PPR repeat
TPR_24	PF23276	361 - 455	8.8e-06	Fungal tetratrico peptide repeats
PPR_long	PF17177	379 - 466	2.2e-06	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	380 - 437	6.6e-10	Pentatricopeptide repeat domain
PPR_1	PF12854	388 - 420	1.4e-07	PPR repeat
PPR_2	PF13041	392 - 439	3.1e-15	PPR repeat family
PPR	PF01535	397 - 425	1.4e-06	PPR repeat
PPR_1	PF12854	423 - 456	3.7e-08	PPR repeat
PPR_2	PF13041	427 - 475	7.5e-15	PPR repeat family
PPR	PF01535	430 - 460	6.7e-07	PPR repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0012815)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G13040 AT1G13040
fragaria_vesca	FvH4_4g25420
malus_domestica	MD13G1103100.v1.1 MD16G1103300.v1.1
prunus_persica	Prupe.1G242700_v2.0.a1
pyrus_communis	pycom13g09010 pycom16g08810
rosa_chinensis	RchiOBHm_Chr4g0432731
rosa_laevigata	RLG00000006817
rosa_multiflora	Rmu_co8522421.1_g000001
rosa_roxburghii	Rroxscaffold_5G00374190
rosa_rugosa	Rorug04G0262000
rosa_samantha	Rh4AG316500 Rh4BG324500 Rh4CG339500 Rh4DG319800
rosa_wichuraiana	Rw4G027530

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	666, 876, 1097
AccB1I	GGYRCC	1 cut(s)	211
AccI	GTMKAC	1 cut(s)	135
AccII	CGCG	2 cut(s)	123, 175
AciI	CCGC	5 cut(s)	25, 43, 121, 145, 681
AclWI	GGATC	1 cut(s)	1470
AcsI	RAATTY	1 cut(s)	280
AdeI	CACNNNGTG	1 cut(s)	775
AfaI	GTAC	2 cut(s)	159, 455
AfeI	AGCGCT	1 cut(s)	1384
AgsI	TTSAA	6 cut(s)	232, 352, 364, 784, 1232, 1533
AjnI	CCWGG	3 cut(s)	87, 248, 567
AjuI	GAANNNNNNNTTGG	2 cut(s)	587, 619
AluBI	AGCT	8 cut(s)	50, 301, 485, 883, 905, 1122, 1141, 1499
AluI	AGCT	8 cut(s)	50, 301, 485, 883, 905, 1122, 1141, 1499
Alw21I	GWGCWC	2 cut(s)	195, 563
Alw26I	GTCTC	3 cut(s)	303, 1424, 1502
Alw44I	GTGCAC	1 cut(s)	559
AlwI	GGATC	1 cut(s)	1470
Aor51HI	AGCGCT	1 cut(s)	1384
AoxI	GGCC	5 cut(s)	67, 262, 542, 564, 892
ApaLI	GTGCAC	1 cut(s)	559
ApeKI	GCWGC	2 cut(s)	1315, 1541
ApoI	RAATTY	1 cut(s)	280
Asp700I	GAANNNNTTC	1 cut(s)	408
AspLEI	GCGC	1 cut(s)	1385
AspS9I	GGNCC	2 cut(s)	565, 892
AsuHPI	GGTGA	3 cut(s)	14, 277, 1267
BaeGI	GKGCMC	2 cut(s)	563, 1064
BanI	GGYRCC	1 cut(s)	211
Bbv12I	GWGCWC	2 cut(s)	195, 563
BbvI	GCAGC	2 cut(s)	1302, 1528
BccI	CCATC	9 cut(s)	412, 464, 847, 940, 995, 1356, 1465, 1487, 1532
BceAI	ACGGC	1 cut(s)	579
BciT130I	CCWGG	3 cut(s)	89, 250, 569
BclI	TGATCA	2 cut(s)	1147, 1204
BcoDI	GTCTC	3 cut(s)	303, 1424, 1502
BfaI	CTAG	3 cut(s)	942, 1262, 1371
BfoI	RGCGCY	1 cut(s)	1386
BisI	GCNGC	4 cut(s)	43, 121, 1316, 1542
BlsI	GCNGC	4 cut(s)	44, 122, 1317, 1543
Bme1390I	CCNGG	3 cut(s)	89, 250, 569
BmgT120I	GGNCC	2 cut(s)	565, 892
BmiI	GGNNCC	4 cut(s)	41, 213, 858, 951
BmrFI	CCNGG	3 cut(s)	89, 250, 569
BmsI	GCATC	2 cut(s)	924, 1528
BpmI	CTGGAG	2 cut(s)	71, 232
BpuEI	CTTGAG	1 cut(s)	1304
Bsa29I	ATCGAT	2 cut(s)	75, 468
BsaAI	YACGTR	1 cut(s)	58
BsaI	GGTCTC	1 cut(s)	1424
BsaJI	CCNNGG	1 cut(s)	567
BsaWI	WCCGGW	2 cut(s)	36, 859
Bse118I	RCCGGY	1 cut(s)	695
Bse1I	ACTGG	2 cut(s)	70, 1024
Bse3DI	GCAATG	3 cut(s)	329, 552, 1167
BseBI	CCWGG	3 cut(s)	89, 250, 569
BseCI	ATCGAT	2 cut(s)	75, 468
BseDI	CCNNGG	1 cut(s)	567
BseGI	GGATG	6 cut(s)	423, 448, 858, 868, 951, 1382
BseMI	GCAATG	3 cut(s)	329, 552, 1167
BseMII	CTCAG	3 cut(s)	810, 1137, 1497
BseNI	ACTGG	2 cut(s)	70, 1024
BseRI	GAGGAG	1 cut(s)	827
BseSI	GKGCMC	2 cut(s)	563, 1064
BseXI	GCAGC	2 cut(s)	1302, 1528
Bsh1236I	CGCG	2 cut(s)	123, 175
BshFI	GGCC	5 cut(s)	69, 264, 544, 566, 894
BshNI	GGYRCC	1 cut(s)	211
BshVI	ATCGAT	2 cut(s)	75, 468
BsiHKAI	GWGCWC	2 cut(s)	195, 563
BsiSI	CCGG	6 cut(s)	37, 440, 545, 696, 755, 860
BsmAI	GTCTC	3 cut(s)	303, 1424, 1502
BsnI	GGCC	5 cut(s)	69, 264, 544, 566, 894
Bso31I	GGTCTC	1 cut(s)	1424
Bsp1286I	GDGCHC	3 cut(s)	195, 563, 1064
Bsp143I	GATC	5 cut(s)	76, 181, 1147, 1204, 1462
BspACI	CCGC	5 cut(s)	25, 43, 121, 145, 681
BspANI	GGCC	5 cut(s)	69, 264, 544, 566, 894
BspCNI	CTCAG	3 cut(s)	811, 1136, 1498
BspDI	ATCGAT	2 cut(s)	75, 468
BspFNI	CGCG	2 cut(s)	123, 175
BspLI	GGNNCC	4 cut(s)	41, 213, 858, 951
BspPI	GGATC	1 cut(s)	1470
BspT107I	GGYRCC	1 cut(s)	211
BspTNI	GGTCTC	1 cut(s)	1424
BsrDI	GCAATG	3 cut(s)	329, 552, 1167
BsrFI	RCCGGY	1 cut(s)	695
BsrI	ACTGG	2 cut(s)	70, 1024
BssAI	RCCGGY	1 cut(s)	695
BssECI	CCNNGG	1 cut(s)	567
BssMI	GATC	5 cut(s)	76, 181, 1147, 1204, 1462
Bst2UI	CCWGG	3 cut(s)	89, 250, 569
Bst4CI	ACNGT	3 cut(s)	1038, 1066, 1181
Bst6I	CTCTTC	2 cut(s)	427, 731
BstBAI	YACGTR	1 cut(s)	58
BstC8I	GCNNGC	2 cut(s)	559, 752
BstDEI	CTNAG	3 cut(s)	819, 1123, 1506
BstF5I	GGATG	6 cut(s)	423, 448, 858, 868, 951, 1382
BstFNI	CGCG	2 cut(s)	123, 175
BstH2I	RGCGCY	1 cut(s)	1386
BstHHI	GCGC	1 cut(s)	1385
BstKTI	GATC	5 cut(s)	79, 184, 1150, 1207, 1465
BstMAI	GTCTC	3 cut(s)	303, 1424, 1502
BstMBI	GATC	5 cut(s)	76, 181, 1147, 1204, 1462
BstMWI	GCNNNNNNNGC	4 cut(s)	24, 129, 153, 563
BstNI	CCWGG	3 cut(s)	89, 250, 569
BstSCI	CCNGG	3 cut(s)	87, 248, 567
BstSLI	GKGCMC	2 cut(s)	563, 1064
BstUI	CGCG	2 cut(s)	123, 175
BstV1I	GCAGC	2 cut(s)	1302, 1528
Bsu15I	ATCGAT	2 cut(s)	75, 468
BsuRI	GGCC	5 cut(s)	69, 264, 544, 566, 894
BsuTUI	ATCGAT	2 cut(s)	75, 468
BtsCI	GGATG	6 cut(s)	423, 448, 858, 868, 951, 1382
BtsIMutI	CAGTG	2 cut(s)	1062, 1258
Cac8I	GCNNGC	2 cut(s)	559, 752
CfoI	GCGC	1 cut(s)	1385
Cfr10I	RCCGGY	1 cut(s)	695
Cfr13I	GGNCC	2 cut(s)	565, 892
ClaI	ATCGAT	2 cut(s)	75, 468
CseI	GACGC	2 cut(s)	121, 1349
Csp6I	GTAC	2 cut(s)	158, 454
CspCI	CAANNNNNGTGG	2 cut(s)	1183, 1218
CviAII	CATG	3 cut(s)	313, 805, 889
CviQI	GTAC	2 cut(s)	158, 454
DdeI	CTNAG	3 cut(s)	819, 1123, 1506
DpnI	GATC	5 cut(s)	78, 183, 1149, 1206, 1464
DpnII	GATC	5 cut(s)	76, 181, 1147, 1204, 1462
DraI	TTTAAA	1 cut(s)	1402
DraIII	CACNNNGTG	1 cut(s)	775
Eam1104I	CTCTTC	2 cut(s)	427, 731
EarI	CTCTTC	2 cut(s)	427, 731
Eco31I	GGTCTC	1 cut(s)	1424
Eco47III	AGCGCT	1 cut(s)	1384
EcoRII	CCWGG	3 cut(s)	87, 248, 567
FaeI	CATG	3 cut(s)	316, 808, 892
FatI	CATG	3 cut(s)	312, 804, 888
FauI	CCCGC	1 cut(s)	674
FbaI	TGATCA	2 cut(s)	1147, 1204
FblI	GTMKAC	1 cut(s)	135
Fnu4HI	GCNGC	4 cut(s)	43, 121, 1316, 1542
FokI	GGATG	6 cut(s)	430, 455, 865, 875, 958, 1389
Fsp4HI	GCNGC	4 cut(s)	43, 121, 1316, 1542
FspBI	CTAG	3 cut(s)	942, 1262, 1371
GlaI	GCGC	1 cut(s)	1384
GluI	GCNGC	4 cut(s)	43, 121, 1316, 1542
GsuI	CTGGAG	2 cut(s)	71, 232
HaeII	RGCGCY	1 cut(s)	1386
HaeIII	GGCC	5 cut(s)	69, 264, 544, 566, 894
HapII	CCGG	6 cut(s)	37, 440, 545, 696, 755, 860
HgaI	GACGC	2 cut(s)	121, 1349
HhaI	GCGC	1 cut(s)	1385
Hin1II	CATG	3 cut(s)	316, 808, 892
Hin6I	GCGC	1 cut(s)	1383
HinP1I	GCGC	1 cut(s)	1383
HincII	GTYRAC	1 cut(s)	136
HindII	GTYRAC	1 cut(s)	136
HindIII	AAGCTT	2 cut(s)	299, 1139
HinfI	GANTC	5 cut(s)	222, 364, 642, 1272, 1510
HpaII	CCGG	6 cut(s)	37, 440, 545, 696, 755, 860
HphI	GGTGA	3 cut(s)	14, 277, 1267
Hpy166II	GTNNAC	5 cut(s)	22, 136, 240, 456, 561
Hpy188I	TCNGA	5 cut(s)	115, 1126, 1152, 1467, 1507
Hpy188III	TCNNGA	4 cut(s)	308, 1135, 1145, 1490
Hpy8I	GTNNAC	5 cut(s)	22, 136, 240, 456, 561
Hpy99I	CGWCG	3 cut(s)	101, 137, 1343
HpyAV	CCTTC	4 cut(s)	589, 628, 1109, 1261
HpyCH4III	ACNGT	3 cut(s)	1038, 1066, 1181
HpyCH4IV	ACGT	1 cut(s)	57
HpyCH4V	TGCA	5 cut(s)	561, 585, 1005, 1366, 1544
HpyF10VI	GCNNNNNNNGC	4 cut(s)	24, 129, 153, 563
HpyF3I	CTNAG	3 cut(s)	819, 1123, 1506
HpySE526I	ACGT	1 cut(s)	57
Hsp92II	CATG	3 cut(s)	316, 808, 892
HspAI	GCGC	1 cut(s)	1383
Ksp22I	TGATCA	2 cut(s)	1147, 1204
Kzo9I	GATC	5 cut(s)	76, 181, 1147, 1204, 1462
LmnI	GCTCC	3 cut(s)	39, 949, 1496
Lsp1109I	GCAGC	2 cut(s)	1302, 1528
LweI	GCATC	2 cut(s)	924, 1528
MaeI	CTAG	3 cut(s)	942, 1262, 1371
MaeII	ACGT	1 cut(s)	57
MaeIII	GTNAC	4 cut(s)	176, 1052, 1078, 1393
MalI	GATC	5 cut(s)	78, 183, 1149, 1206, 1464
MboI	GATC	5 cut(s)	76, 181, 1147, 1204, 1462
MboII	GAAGA	5 cut(s)	296, 359, 444, 748, 844
MhlI	GDGCHC	3 cut(s)	195, 563, 1064
MluCI	AATT	9 cut(s)	280, 404, 462, 491, 646, 784, 1403, 1410, 1528
MlyI	GAGTC	2 cut(s)	651, 1504
MmeI	TCCRAC	1 cut(s)	789
MroXI	GAANNNNTTC	1 cut(s)	408
MseI	TTAA	4 cut(s)	1109, 1308, 1401, 1518
MslI	CAYNNNNRTG	1 cut(s)	1353
MspI	CCGG	6 cut(s)	37, 440, 545, 696, 755, 860
MspR9I	CCNGG	3 cut(s)	89, 250, 569
MvaI	CCWGG	3 cut(s)	89, 250, 569
MvnI	CGCG	2 cut(s)	123, 175
MwoI	GCNNNNNNNGC	4 cut(s)	24, 129, 153, 563
NdeII	GATC	5 cut(s)	76, 181, 1147, 1204, 1462
NlaIII	CATG	3 cut(s)	316, 808, 892
NlaIV	GGNNCC	4 cut(s)	41, 213, 858, 951
NmeAIII	GCCGAG	1 cut(s)	214
PcsI	WCGNNNNNNNCGW	2 cut(s)	159, 186
PdmI	GAANNNNTTC	1 cut(s)	408
PfeI	GAWTC	3 cut(s)	222, 364, 1272
PflFI	GACNNNGTC	1 cut(s)	617
PkrI	GCNGC	4 cut(s)	44, 122, 1317, 1543
PleI	GAGTC	2 cut(s)	650, 1504
PpsI	GAGTC	2 cut(s)	650, 1504
Ppu21I	YACGTR	1 cut(s)	58
PsiI	TTATAA	3 cut(s)	666, 876, 1097
Psp6I	CCWGG	3 cut(s)	87, 248, 567
PspGI	CCWGG	3 cut(s)	87, 248, 567
PspN4I	GGNNCC	4 cut(s)	41, 213, 858, 951
PspPI	GGNCC	2 cut(s)	565, 892
PsyI	GACNNNGTC	1 cut(s)	617
RsaI	GTAC	2 cut(s)	159, 455
RsaNI	GTAC	2 cut(s)	158, 454
RseI	CAYNNNNRTG	1 cut(s)	1353
SalI	GTCGAC	1 cut(s)	134
SaqAI	TTAA	4 cut(s)	1109, 1308, 1401, 1518
SatI	GCNGC	4 cut(s)	43, 121, 1316, 1542
Sau3AI	GATC	5 cut(s)	76, 181, 1147, 1204, 1462
Sau96I	GGNCC	2 cut(s)	565, 892
SchI	GAGTC	2 cut(s)	651, 1504
ScrFI	CCNGG	3 cut(s)	89, 250, 569
SduI	GDGCHC	3 cut(s)	195, 563, 1064
SfaNI	GCATC	2 cut(s)	924, 1528
SmiMI	CAYNNNNRTG	1 cut(s)	1353
SmlI	CTYRAG	1 cut(s)	1319
SmoI	CTYRAG	1 cut(s)	1319
Sse9I	AATT	9 cut(s)	280, 404, 462, 491, 646, 784, 1403, 1410, 1528
SsiI	CCGC	5 cut(s)	25, 43, 121, 145, 681
SspI	AATATT	1 cut(s)	1228
SspMI	CTAG	3 cut(s)	942, 1262, 1371
StyD4I	CCNGG	3 cut(s)	87, 248, 567
TaaI	ACNGT	3 cut(s)	1038, 1066, 1181
TaiI	ACGT	1 cut(s)	60
TaqI	TCGA	6 cut(s)	7, 75, 96, 135, 307, 468
TasI	AATT	9 cut(s)	280, 404, 462, 491, 646, 784, 1403, 1410, 1528
TauI	GCSGC	2 cut(s)	45, 123
TfiI	GAWTC	3 cut(s)	222, 364, 1272
Tru1I	TTAA	4 cut(s)	1109, 1308, 1401, 1518
Tru9I	TTAA	4 cut(s)	1109, 1308, 1401, 1518
TscAI	CASTG	2 cut(s)	1069, 1258
TseI	GCWGC	2 cut(s)	1315, 1541
TspDTI	ATGAA	9 cut(s)	139, 244, 401, 642, 647, 749, 793, 845, 1281
TspGWI	ACGGA	1 cut(s)	404
TspRI	CASTG	2 cut(s)	1069, 1258
Tth111I	GACNNNGTC	1 cut(s)	617
VneI	GTGCAC	1 cut(s)	559
XapI	RAATTY	1 cut(s)	280
XmiI	GTMKAC	1 cut(s)	135
XmnI	GAANNNNTTC	1 cut(s)	408
XspI	CTAG	3 cut(s)	942, 1262, 1371

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.