pycom16g10840
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA M5U methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0013116
Physical Location & Seq
Forward (+)
7469664 .. 7470439
776 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 369 bp
ATGCTGCCTCTTCAGATTGTTGGCAAACAAATTATACTGAGTTGGCACATCTTAATCTCTTCCCATTTCGAACACGACATGCGGAGAAGGAGTGAGGGTTGGTGCCTCTCTGCTTCCTTTCCGTTTGTCCTCCTCCGCCTCCACTCGTCACCTGCCACGTCTCCCCAACCACAGTGCACCCCCCGGCGCACCTCCACAAGTACAGCAGCACTACCTTCTCTCTCATTGAGCCCTAACTCAACCCCTCCTCCGCTGCCGCTAACCTGCGCCCTCCACAGCCCGCACTTCGAATCGTGCTCCAGGTGTACACACGAGTTCAATCTCCACCGTCTGGTCATAATCAATGAGGCGATAGAGTTCTTTTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cons_hypoth95 MTS

Protein Analysis

123

Amino Acids

13.7

Weight (kDa)

8.42

Isoelectric Point (pI)

91.69

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013116)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G27180
fragaria_vesca FvH4_6g53250
malus_domestica MD09G1012500.v1.1
prunus_persica Prupe.3G304500_v2.0.a1
pyrus_communis pycom02g22280 pycom111g01050 pycom16g10840
rosa_chinensis RchiOBHm_Chr2g0175381
rosa_laevigata RLG00000022323
rosa_multiflora Rmu_sc0003512.1_g000009
rosa_roxburghii Rroxscaffold_2G00077420
rosa_rugosa Rorug02G0583500 Rorug02G0583600
rosa_samantha Rh2AG663700 Rh2BG674500 Rh2CG637700 Rh2DG688500
rosa_wichuraiana Rw2G054390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 160
Acc36I ACCTGC 2 cut(s) 160, 272
AccB1I GGYRCC 1 cut(s) 102
AccB7I CCANNNNNTGG 1 cut(s) 331
AciI CCGC 5 cut(s) 82, 136, 251, 257, 281
AfaI GTAC 2 cut(s) 202, 307
AfiI CCNNNNNNNGG 1 cut(s) 331
AgsI TTSAA 1 cut(s) 319
AjiI CACGTC 1 cut(s) 159
AjnI CCWGG 1 cut(s) 299
Alw21I GWGCWC 2 cut(s) 179, 299
Alw26I GTCTC 1 cut(s) 165
Alw44I GTGCAC 1 cut(s) 175
ApaLI GTGCAC 1 cut(s) 175
ApeKI GCWGC 3 cut(s) 4, 206, 253
AspLEI GCGC 2 cut(s) 189, 269
AsuC2I CCSGG 1 cut(s) 184
AsuHPI GGTGA 1 cut(s) 141
AsuII TTCGAA 2 cut(s) 69, 288
BaeGI GKGCMC 1 cut(s) 179
BanI GGYRCC 1 cut(s) 102
BanII GRGCYC 1 cut(s) 233
BauI CACGAG 1 cut(s) 311
Bbv12I GWGCWC 2 cut(s) 179, 299
BbvI GCAGC 2 cut(s) 218, 240
BciT130I CCWGG 1 cut(s) 301
BcnI CCSGG 1 cut(s) 184
BcoDI GTCTC 1 cut(s) 165
BfuAI ACCTGC 2 cut(s) 160, 272
BisI GCNGC 4 cut(s) 5, 207, 254, 257
BlsI GCNGC 4 cut(s) 6, 208, 255, 258
Bme1390I CCNGG 2 cut(s) 184, 301
BmgBI CACGTC 1 cut(s) 159
BmiI GGNNCC 1 cut(s) 104
BmrFI CCNGG 2 cut(s) 184, 301
BpmI CTGGAG 1 cut(s) 283
Bpu14I TTCGAA 2 cut(s) 69, 288
BpuMI CCSGG 1 cut(s) 184
BsaJI CCNNGG 1 cut(s) 182
BsaXI ACNNNNNCTCC 6 cut(s) 76, 82, 106, 112, 232, 262
Bsc4I CCNNNNNNNGG 1 cut(s) 331
BseBI CCWGG 1 cut(s) 301
BseDI CCNNGG 1 cut(s) 182
BseLI CCNNNNNNNGG 1 cut(s) 331
BseMII CTCAG 1 cut(s) 29
BseRI GAGGAG 2 cut(s) 122, 237
BseSI GKGCMC 1 cut(s) 179
BseXI GCAGC 2 cut(s) 218, 240
BshNI GGYRCC 1 cut(s) 102
BsiHKAI GWGCWC 2 cut(s) 179, 299
BsiSI CCGG 1 cut(s) 184
BslI CCNNNNNNNGG 1 cut(s) 331
BsmAI GTCTC 1 cut(s) 165
BsmBI CGTCTC 1 cut(s) 165
Bsp119I TTCGAA 2 cut(s) 69, 288
Bsp1286I GDGCHC 3 cut(s) 179, 233, 299
Bsp1407I TGTACA 1 cut(s) 305
BspACI CCGC 5 cut(s) 82, 136, 251, 257, 281
BspCNI CTCAG 1 cut(s) 30
BspLI GGNNCC 1 cut(s) 104
BspMI ACCTGC 2 cut(s) 160, 272
BspT104I TTCGAA 2 cut(s) 69, 288
BspT107I GGYRCC 1 cut(s) 102
BsrGI TGTACA 1 cut(s) 305
BssECI CCNNGG 1 cut(s) 182
BssSI CACGAG 1 cut(s) 311
Bst2BI CACGAG 1 cut(s) 311
Bst2UI CCWGG 1 cut(s) 301
Bst4CI ACNGT 2 cut(s) 174, 329
Bst6I CTCTTC 2 cut(s) 15, 64
BstAUI TGTACA 1 cut(s) 305
BstBI TTCGAA 2 cut(s) 69, 288
BstC8I GCNNGC 1 cut(s) 281
BstDEI CTNAG 1 cut(s) 38
BstHHI GCGC 2 cut(s) 189, 269
BstMAI GTCTC 1 cut(s) 165
BstNI CCWGG 1 cut(s) 301
BstNSI RCATGY 1 cut(s) 82
BstSCI CCNGG 2 cut(s) 182, 299
BstSLI GKGCMC 1 cut(s) 179
BstV1I GCAGC 2 cut(s) 218, 240
BtrI CACGTC 1 cut(s) 159
BtsIMutI CAGTG 1 cut(s) 179
BveI ACCTGC 2 cut(s) 160, 272
Cac8I GCNNGC 1 cut(s) 281
CfoI GCGC 2 cut(s) 189, 269
Csp6I GTAC 2 cut(s) 201, 306
CviAII CATG 1 cut(s) 79
CviJI RGCY 2 cut(s) 231, 279
CviKI_1 RGCY 2 cut(s) 231, 279
CviQI GTAC 2 cut(s) 201, 306
DdeI CTNAG 1 cut(s) 38
Eam1104I CTCTTC 2 cut(s) 15, 64
EarI CTCTTC 2 cut(s) 15, 64
EciI GGCGGA 1 cut(s) 125
Eco24I GRGCYC 1 cut(s) 233
EcoRII CCWGG 1 cut(s) 299
EcoT38I GRGCYC 1 cut(s) 233
Esp3I CGTCTC 1 cut(s) 165
FaeI CATG 1 cut(s) 82
FaiI YATR 3 cut(s) 35, 80, 338
FatI CATG 1 cut(s) 78
FauI CCCGC 1 cut(s) 288
Fnu4HI GCNGC 4 cut(s) 5, 207, 254, 257
FriOI GRGCYC 1 cut(s) 233
Fsp4HI GCNGC 4 cut(s) 5, 207, 254, 257
GlaI GCGC 2 cut(s) 188, 268
GluI GCNGC 4 cut(s) 5, 207, 254, 257
GsuI CTGGAG 1 cut(s) 283
HapII CCGG 1 cut(s) 184
HhaI GCGC 2 cut(s) 189, 269
Hin1II CATG 1 cut(s) 82
Hin6I GCGC 2 cut(s) 187, 267
HinP1I GCGC 2 cut(s) 187, 267
HinfI GANTC 1 cut(s) 290
HpaII CCGG 1 cut(s) 184
HphI GGTGA 1 cut(s) 141
Hpy166II GTNNAC 3 cut(s) 177, 306, 308
Hpy188I TCNGA 1 cut(s) 15
Hpy8I GTNNAC 3 cut(s) 177, 306, 308
HpyAV CCTTC 2 cut(s) 81, 225
HpyCH4III ACNGT 2 cut(s) 174, 329
HpyCH4IV ACGT 1 cut(s) 158
HpyCH4V TGCA 1 cut(s) 177
HpyF3I CTNAG 1 cut(s) 38
HpySE526I ACGT 1 cut(s) 158
Hsp92II CATG 1 cut(s) 82
HspAI GCGC 2 cut(s) 187, 267
LmnI GCTCC 1 cut(s) 302
LpnPI CCDG 6 cut(s) 165, 197, 277, 286, 313, 317
Lsp1109I GCAGC 2 cut(s) 218, 240
MaeII ACGT 1 cut(s) 158
MaeIII GTNAC 1 cut(s) 147
MboII GAAGA 1 cut(s) 51
MhlI GDGCHC 3 cut(s) 179, 233, 299
MluCI AATT 1 cut(s) 30
MseI TTAA 2 cut(s) 53, 367
MspA1I CMGCKG 1 cut(s) 253
MspI CCGG 1 cut(s) 184
MspR9I CCNGG 2 cut(s) 184, 301
MvaI CCWGG 1 cut(s) 301
NciI CCSGG 1 cut(s) 184
NlaIII CATG 1 cut(s) 82
NlaIV GGNNCC 1 cut(s) 104
NmuCI GTSAC 1 cut(s) 147
NspI RCATGY 1 cut(s) 82
NspV TTCGAA 2 cut(s) 69, 288
PaqCI CACCTGC 1 cut(s) 160
PfeI GAWTC 1 cut(s) 290
PflMI CCANNNNNTGG 1 cut(s) 331
PkrI GCNGC 4 cut(s) 6, 208, 255, 258
Psp6I CCWGG 1 cut(s) 299
PspGI CCWGG 1 cut(s) 299
PspN4I GGNNCC 1 cut(s) 104
RsaI GTAC 2 cut(s) 202, 307
RsaNI GTAC 2 cut(s) 201, 306
SaqAI TTAA 2 cut(s) 53, 367
SatI GCNGC 4 cut(s) 5, 207, 254, 257
ScrFI CCNGG 2 cut(s) 184, 301
SduI GDGCHC 3 cut(s) 179, 233, 299
SetI ASST 6 cut(s) 154, 161, 194, 217, 266, 305
SfuI TTCGAA 2 cut(s) 69, 288
Sse9I AATT 1 cut(s) 30
SsiI CCGC 5 cut(s) 82, 136, 251, 257, 281
StyD4I CCNGG 2 cut(s) 182, 299
TaaI ACNGT 2 cut(s) 174, 329
TaiI ACGT 1 cut(s) 161
TaqI TCGA 2 cut(s) 69, 288
TasI AATT 1 cut(s) 30
TatI WGTACW 2 cut(s) 200, 305
TauI GCSGC 1 cut(s) 259
TfiI GAWTC 1 cut(s) 290
Tru1I TTAA 2 cut(s) 53, 367
Tru9I TTAA 2 cut(s) 53, 367
TscAI CASTG 1 cut(s) 179
TseFI GTSAC 1 cut(s) 147
TseI GCWGC 3 cut(s) 4, 206, 253
Tsp45I GTSAC 1 cut(s) 147
TspGWI ACGGA 1 cut(s) 111
TspRI CASTG 1 cut(s) 179
Van91I CCANNNNNTGG 1 cut(s) 331
VneI GTGCAC 1 cut(s) 175
XceI RCATGY 1 cut(s) 82
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.