pycom16g11500

Heparan-alpha-glucosaminide N-acetyltransferase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0003628
Physical Location & Seq
Reverse (-)
8014864 .. 8020185
5322 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1437 bp
ATGGCGATGTACGAGCCTATTAAAGTGTACTTGGACGATGAAAAACGTGGTATGATGGATCTTATCATGGGTAAGAACGTTAATATCGGTGGTGGTGGTGGTGCCGGCGGTGATGGTCATATGGACGATGATGTTGAGATGGCCGTCCAGCCGATATCAAAGCCTCCCACCGGCGTCGGGGGCGCCGCTTGTAAGAATATTAGTAACGGAGAAGCAGCGGTGCCGTCATTGCCTTCGAAGTCGTGGCAACAGCAGCAACGCCTTGTTTCCCTTGACGTCTTTCGTGGACTCACGGTTGCGCTGATGATAATAGTGGACGACGTGGGTGGAATCCTGCCTGCAATTAACCATTCACCTTGGAATGGTTTAACCCTTGCAGATTTAGTCATGCCATTTTTCCTGTTTATTGTTGGTCTTTCCCTTGCACTTGCCTACAAGAAACCGTCATGCGGAGCCGATGCTACGAAAAAAGCAGTTTTACGGACGCTGAAGCTTCTAGCATTAGGCCTTTTTCTTCAAGGCGGATTTTTCCATCGCGTCAAGGATCTTACTTTCGGGGTTGATATCACAAAGATGAGATGGATGGGCATTCTACAGAGAATTGCGATTGGATATATTGTAGCAGCATTGTGCGAGATTTGGCTAAAGCGTGATAGTAATGTCAACTCAGGAAGATCGCTGCTCAGGAAATATCGATCTCAATTGGCGGTGGCATTGATAATCACCACGATATACCTGTCATTGTTATACGGATTATATGTTCCTGATTGGGAGTACCAGATTCCTGTCGATTCCTCCTCTGCACCAAAAACATTTTCAGTAAAATGTGGAGTACGGGGTGACACAGGATCGGCTTGCAATGCAGTTGGGATGATTGATCGTACGATATTGGGGATTGAGCATTTATATAAAAGACCAGTCTACGCACGAACGGAGCAATGCAGTATTAACTCCCCTGATTATGGCCCGTTGCCTGCCGATGCTCCTTCTTGGTGCCAAGCCCCTTTTGATCCTGAAGGACTTCTGAGTTCAATGATGGCTATTGTTACCTGCTTGGTTGGTTTGCACTACGGGCACATCATTGTCCATTTTAAGGATCACCGGGAAAGAATCCTACACTGGTCTATTTCATCCTCGTCTCTTGTAGTCTTAGGCCTCACCCTCGACTTAATTGGCATGCATATTAACAAGGCTCTTTACACATTCAGTTATATGTGTATCACAGCTGGTGCTGCTGGAATTCTCTTCGCTGCAATTTACTTGATGGTCGATGTGTGTGGCTATAGGCGCCTGGCTATTGTGATGGAATGGATGGGAATGCATGCACTGATGATTTTCGTACTCATAGCCTGCAATATATTGCCAGTTGTCCTCTTTGGATTCTATTGGGGGAAGCCTGAGAACAACATTCTTACCTTGATTGGAATCGGAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00531 ko01100 ko04142 map00531 map01100 map04142
Pfam Domains
Protein Families
DUF1624

Protein Analysis

479

Amino Acids

52.21

Weight (kDa)

8.38

Isoelectric Point (pI)

48.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HGSNAT_cat PF07786 87 - 211 1.5e-07 Heparan-alpha-glucosaminide N-acetyltransferase, catalytic
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 279
Acc36I ACCTGC 1 cut(s) 1058
AccB1I GGYRCC 5 cut(s) 101, 182, 220, 993, 1287
AccI GTMKAC 1 cut(s) 921
AccII CGCG 1 cut(s) 537
AciI CCGC 6 cut(s) 108, 186, 218, 450, 522, 707
AclI AACGTT 1 cut(s) 78
AclWI GGATC 5 cut(s) 66, 552, 856, 1004, 1104
AcoI YGGCCR 1 cut(s) 141
AcsI RAATTY 1 cut(s) 1239
AcuI CTGAAG 2 cut(s) 509, 1035
AcyI GRCGYC 4 cut(s) 174, 183, 276, 1288
AfaI GTAC 6 cut(s) 11, 29, 776, 834, 883, 1341
AfiI CCNNNNNNNGG 6 cut(s) 170, 177, 362, 449, 962, 1093
AgsI TTSAA 2 cut(s) 518, 1032
AjiI CACGTC 1 cut(s) 322
AjnI CCWGG 1 cut(s) 1290
AjuI GAANNNNNNNTTGG 2 cut(s) 799, 831
AloI GAACNNNNNNTCC 2 cut(s) 744, 776
AluBI AGCT 2 cut(s) 493, 1226
AluI AGCT 2 cut(s) 493, 1226
Alw26I GTCTC 1 cut(s) 1143
AlwI GGATC 5 cut(s) 66, 552, 856, 1004, 1104
AoxI GGCC 4 cut(s) 141, 505, 964, 1153
ApeKI GCWGC 6 cut(s) 215, 253, 623, 679, 1232, 1250
ApoI RAATTY 1 cut(s) 1239
Asp700I GAANNNNTTC 1 cut(s) 1020
AspLEI GCGC 3 cut(s) 185, 301, 1290
AspS9I GGNCC 1 cut(s) 965
AsuC2I CCSGG 1 cut(s) 1103
AsuHPI GGTGA 6 cut(s) 122, 345, 715, 851, 1091, 1150
AsuII TTCGAA 1 cut(s) 236
BaeGI GKGCMC 1 cut(s) 1077
BanI GGYRCC 5 cut(s) 101, 182, 220, 993, 1287
BbvI GCAGC 6 cut(s) 227, 265, 635, 666, 1219, 1237
BceAI ACGGC 2 cut(s) 128, 208
BciT130I CCWGG 1 cut(s) 1292
BcnI CCSGG 1 cut(s) 1103
BcoDI GTCTC 1 cut(s) 1143
BfaI CTAG 1 cut(s) 497
BfmI CTRYAG 2 cut(s) 593, 1282
BfoI RGCGCY 2 cut(s) 186, 1291
BfuAI ACCTGC 1 cut(s) 1058
BisI GCNGC 7 cut(s) 186, 216, 254, 624, 680, 1233, 1251
BlsI GCNGC 7 cut(s) 187, 217, 255, 625, 681, 1234, 1252
Bme1390I CCNGG 2 cut(s) 1103, 1292
BmgBI CACGTC 1 cut(s) 322
BmgT120I GGNCC 1 cut(s) 965
BmiI GGNNCC 6 cut(s) 103, 184, 222, 454, 995, 1289
BmrFI CCNGG 2 cut(s) 1103, 1292
BmsI GCATC 2 cut(s) 448, 970
Bpu10I CCTNAGC 1 cut(s) 683
Bpu14I TTCGAA 1 cut(s) 236
BpuMI CCSGG 1 cut(s) 1103
Bsa29I ATCGAT 1 cut(s) 694
BsaBI GATNNNNATC 1 cut(s) 1424
BsaHI GRCGYC 4 cut(s) 174, 183, 276, 1288
BsaJI CCNNGG 1 cut(s) 356
Bsc4I CCNNNNNNNGG 6 cut(s) 170, 177, 362, 449, 962, 1093
Bse118I RCCGGY 2 cut(s) 104, 170
Bse1I ACTGG 3 cut(s) 917, 1124, 1364
Bse3DI GCAATG 3 cut(s) 227, 865, 944
Bse8I GATNNNNATC 1 cut(s) 1424
BseBI CCWGG 1 cut(s) 1292
BseCI ATCGAT 1 cut(s) 694
BseDI CCNNGG 1 cut(s) 356
BseGI GGATG 4 cut(s) 588, 876, 1130, 1317
BseJI GATNNNNATC 1 cut(s) 1424
BseLI CCNNNNNNNGG 6 cut(s) 170, 177, 362, 449, 962, 1093
BseMI GCAATG 3 cut(s) 227, 865, 944
BseMII CTCAG 4 cut(s) 681, 697, 1016, 1389
BseNI ACTGG 3 cut(s) 917, 1124, 1364
BseRI GAGGAG 1 cut(s) 787
BseSI GKGCMC 1 cut(s) 1077
BseXI GCAGC 6 cut(s) 227, 265, 635, 666, 1219, 1237
BsgI GTGCAG 1 cut(s) 786
Bsh1236I CGCG 1 cut(s) 537
BshFI GGCC 4 cut(s) 143, 507, 966, 1155
BshNI GGYRCC 5 cut(s) 101, 182, 220, 993, 1287
BshVI ATCGAT 1 cut(s) 694
BsiSI CCGG 3 cut(s) 105, 171, 1102
BsiWI CGTACG 1 cut(s) 881
BslI CCNNNNNNNGG 6 cut(s) 170, 177, 362, 449, 962, 1093
BsmAI GTCTC 1 cut(s) 1143
BsmBI CGTCTC 1 cut(s) 1143
BsmI GAATGC 2 cut(s) 588, 1323
BsnI GGCC 4 cut(s) 143, 507, 966, 1155
Bsp119I TTCGAA 1 cut(s) 236
Bsp1286I GDGCHC 1 cut(s) 1077
Bsp143I GATC 8 cut(s) 58, 544, 674, 695, 848, 877, 1009, 1096
BspACI CCGC 6 cut(s) 108, 186, 218, 450, 522, 707
BspANI GGCC 4 cut(s) 143, 507, 966, 1155
BspCNI CTCAG 4 cut(s) 680, 696, 1017, 1390
BspDI ATCGAT 1 cut(s) 694
BspFNI CGCG 1 cut(s) 537
BspLI GGNNCC 6 cut(s) 103, 184, 222, 454, 995, 1289
BspMI ACCTGC 1 cut(s) 1058
BspPI GGATC 5 cut(s) 66, 552, 856, 1004, 1104
BspT104I TTCGAA 1 cut(s) 236
BspT107I GGYRCC 5 cut(s) 101, 182, 220, 993, 1287
BsrDI GCAATG 3 cut(s) 227, 865, 944
BsrFI RCCGGY 2 cut(s) 104, 170
BsrI ACTGG 3 cut(s) 917, 1124, 1364
BssAI RCCGGY 2 cut(s) 104, 170
BssECI CCNNGG 1 cut(s) 356
BssMI GATC 8 cut(s) 58, 544, 674, 695, 848, 877, 1009, 1096
BssNI GRCGYC 4 cut(s) 174, 183, 276, 1288
BssT1I CCWWGG 1 cut(s) 356
Bst2UI CCWGG 1 cut(s) 1292
Bst4CI ACNGT 2 cut(s) 295, 444
Bst6I CTCTTC 1 cut(s) 1250
BstACI GRCGYC 4 cut(s) 174, 183, 276, 1288
BstBI TTCGAA 1 cut(s) 236
BstC8I GCNNGC 7 cut(s) 106, 339, 856, 975, 1178, 1323, 1351
BstDEI CTNAG 5 cut(s) 667, 683, 1025, 1150, 1398
BstF5I GGATG 4 cut(s) 588, 876, 1130, 1317
BstFNI CGCG 1 cut(s) 537
BstH2I RGCGCY 2 cut(s) 186, 1291
BstHHI GCGC 3 cut(s) 185, 301, 1290
BstKTI GATC 8 cut(s) 61, 547, 677, 698, 851, 880, 1012, 1099
BstMAI GTCTC 1 cut(s) 1143
BstMBI GATC 8 cut(s) 58, 544, 674, 695, 848, 877, 1009, 1096
BstMWI GCNNNNNNNGC 7 cut(s) 180, 229, 253, 860, 1072, 1232, 1287
BstNI CCWGG 1 cut(s) 1292
BstNSI RCATGY 2 cut(s) 1180, 1325
BstSCI CCNGG 2 cut(s) 1101, 1290
BstSFI CTRYAG 2 cut(s) 593, 1282
BstSLI GKGCMC 1 cut(s) 1077
BstUI CGCG 1 cut(s) 537
BstV1I GCAGC 6 cut(s) 227, 265, 635, 666, 1219, 1237
BstX2I RGATCY 2 cut(s) 58, 544
BstYI RGATCY 2 cut(s) 58, 544
Bsu15I ATCGAT 1 cut(s) 694
BsuRI GGCC 4 cut(s) 143, 507, 966, 1155
BsuTUI ATCGAT 1 cut(s) 694
BtgZI GCGATG 2 cut(s) 20, 518
BtrI CACGTC 1 cut(s) 322
BtsCI GGATG 4 cut(s) 588, 876, 1130, 1317
BtsIMutI CAGTG 2 cut(s) 1117, 1325
BveI ACCTGC 1 cut(s) 1058
Cac8I GCNNGC 7 cut(s) 106, 339, 856, 975, 1178, 1323, 1351
CfoI GCGC 3 cut(s) 185, 301, 1290
Cfr10I RCCGGY 2 cut(s) 104, 170
Cfr13I GGNCC 1 cut(s) 965
ClaI ATCGAT 1 cut(s) 694
CseI GACGC 3 cut(s) 163, 493, 526
Csp6I GTAC 6 cut(s) 10, 28, 775, 833, 882, 1340
CviAII CATG 5 cut(s) 67, 388, 447, 1177, 1322
CviQI GTAC 6 cut(s) 10, 28, 775, 833, 882, 1340
DdeI CTNAG 5 cut(s) 667, 683, 1025, 1150, 1398
DinI GGCGCC 2 cut(s) 184, 1289
DpnI GATC 8 cut(s) 60, 546, 676, 697, 850, 879, 1011, 1098
DpnII GATC 8 cut(s) 58, 544, 674, 695, 848, 877, 1009, 1096
EaeI YGGCCR 1 cut(s) 141
Eam1104I CTCTTC 1 cut(s) 1250
EarI CTCTTC 1 cut(s) 1250
EciI GGCGGA 1 cut(s) 537
Eco130I CCWWGG 1 cut(s) 356
Eco147I AGGCCT 2 cut(s) 507, 1155
Eco32I GATATC 2 cut(s) 156, 565
Eco57I CTGAAG 2 cut(s) 509, 1035
EcoRI GAATTC 1 cut(s) 1239
EcoRII CCWGG 1 cut(s) 1290
EcoRV GATATC 2 cut(s) 156, 565
EcoT14I CCWWGG 1 cut(s) 356
EcoT22I ATGCAT 2 cut(s) 1182, 1323
EgeI GGCGCC 2 cut(s) 184, 1289
EheI GGCGCC 2 cut(s) 184, 1289
ErhI CCWWGG 1 cut(s) 356
Esp3I CGTCTC 1 cut(s) 1143
FaeI CATG 5 cut(s) 70, 391, 450, 1180, 1325
FatI CATG 5 cut(s) 66, 387, 446, 1176, 1321
FauNDI CATATG 1 cut(s) 120
FblI GTMKAC 1 cut(s) 921
Fnu4HI GCNGC 7 cut(s) 186, 216, 254, 624, 680, 1233, 1251
FokI GGATG 4 cut(s) 595, 883, 1117, 1324
Fsp4HI GCNGC 7 cut(s) 186, 216, 254, 624, 680, 1233, 1251
FspBI CTAG 1 cut(s) 497
GlaI GCGC 3 cut(s) 184, 300, 1289
GluI GCNGC 7 cut(s) 186, 216, 254, 624, 680, 1233, 1251
HaeII RGCGCY 2 cut(s) 186, 1291
HaeIII GGCC 4 cut(s) 143, 507, 966, 1155
HapII CCGG 3 cut(s) 105, 171, 1102
HgaI GACGC 3 cut(s) 163, 493, 526
HhaI GCGC 3 cut(s) 185, 301, 1290
Hin1I GRCGYC 4 cut(s) 174, 183, 276, 1288
Hin1II CATG 5 cut(s) 70, 391, 450, 1180, 1325
Hin6I GCGC 3 cut(s) 183, 299, 1288
HinP1I GCGC 3 cut(s) 183, 299, 1288
HincII GTYRAC 1 cut(s) 664
HindII GTYRAC 1 cut(s) 664
HindIII AAGCTT 1 cut(s) 491
HinfI GANTC 7 cut(s) 288, 330, 781, 791, 1110, 1380, 1425
HpaII CCGG 3 cut(s) 105, 171, 1102
HphI GGTGA 6 cut(s) 122, 345, 715, 851, 1091, 1150
Hpy166II GTNNAC 5 cut(s) 28, 287, 316, 664, 922
Hpy188I TCNGA 2 cut(s) 1026, 1430
Hpy188III TCNNGA 4 cut(s) 669, 685, 764, 1013
Hpy8I GTNNAC 5 cut(s) 28, 287, 316, 664, 922
Hpy99I CGWCG 2 cut(s) 179, 323
HpyAV CCTTC 3 cut(s) 243, 996, 1010
HpyCH4III ACNGT 2 cut(s) 295, 444
HpyCH4IV ACGT 4 cut(s) 46, 78, 276, 321
HpyF10VI GCNNNNNNNGC 7 cut(s) 180, 229, 253, 860, 1072, 1232, 1287
HpyF3I CTNAG 5 cut(s) 667, 683, 1025, 1150, 1398
HpySE526I ACGT 4 cut(s) 46, 78, 276, 321
Hsp92I GRCGYC 4 cut(s) 174, 183, 276, 1288
Hsp92II CATG 5 cut(s) 70, 391, 450, 1180, 1325
HspAI GCGC 3 cut(s) 183, 299, 1288
KasI GGCGCC 2 cut(s) 182, 1287
KroI GCCGGC 1 cut(s) 104
KroNI GCCGGC 1 cut(s) 106
Kzo9I GATC 8 cut(s) 58, 544, 674, 695, 848, 877, 1009, 1096
LmnI GCTCC 3 cut(s) 452, 934, 988
Lsp1109I GCAGC 6 cut(s) 227, 265, 635, 666, 1219, 1237
LweI GCATC 2 cut(s) 448, 970
MaeI CTAG 1 cut(s) 497
MaeII ACGT 4 cut(s) 46, 78, 276, 321
MaeIII GTNAC 3 cut(s) 203, 839, 1045
MalI GATC 8 cut(s) 60, 546, 676, 697, 850, 879, 1011, 1098
MboI GATC 8 cut(s) 58, 544, 674, 695, 848, 877, 1009, 1096
MboII GAAGA 3 cut(s) 506, 684, 1237
MfeI CAATTG 1 cut(s) 701
MflI RGATCY 2 cut(s) 58, 544
MhlI GDGCHC 1 cut(s) 1077
MluCI AATT 6 cut(s) 342, 600, 701, 1170, 1239, 1254
Mly113I GGCGCC 2 cut(s) 183, 1288
MlyI GAGTC 1 cut(s) 282
MnlI CCTC 7 cut(s) 174, 805, 808, 1144, 1166, 1172, 1382
Mph1103I ATGCAT 2 cut(s) 1182, 1323
MroNI GCCGGC 1 cut(s) 104
MroXI GAANNNNTTC 1 cut(s) 1020
MseI TTAA 8 cut(s) 21, 81, 345, 368, 948, 1092, 1169, 1185
MslI CAYNNNNRTG 1 cut(s) 572
MspA1I CMGCKG 2 cut(s) 218, 1226
MspI CCGG 3 cut(s) 105, 171, 1102
MspR9I CCNGG 2 cut(s) 1103, 1292
MunI CAATTG 1 cut(s) 701
Mva1269I GAATGC 2 cut(s) 588, 1323
MvaI CCWGG 1 cut(s) 1292
MvnI CGCG 1 cut(s) 537
MwoI GCNNNNNNNGC 7 cut(s) 180, 229, 253, 860, 1072, 1232, 1287
NaeI GCCGGC 1 cut(s) 106
NarI GGCGCC 2 cut(s) 183, 1288
NciI CCSGG 1 cut(s) 1103
NdeI CATATG 1 cut(s) 120
NdeII GATC 8 cut(s) 58, 544, 674, 695, 848, 877, 1009, 1096
NgoMIV GCCGGC 1 cut(s) 104
NlaIII CATG 5 cut(s) 70, 391, 450, 1180, 1325
NlaIV GGNNCC 6 cut(s) 103, 184, 222, 454, 995, 1289
NmuCI GTSAC 1 cut(s) 839
NsiI ATGCAT 2 cut(s) 1182, 1323
NspI RCATGY 2 cut(s) 1180, 1325
NspV TTCGAA 1 cut(s) 236
PaeI GCATGC 2 cut(s) 1180, 1325
PceI AGGCCT 2 cut(s) 507, 1155
PctI GAATGC 2 cut(s) 588, 1323
PdiI GCCGGC 1 cut(s) 106
PdmI GAANNNNTTC 1 cut(s) 1020
PfeI GAWTC 6 cut(s) 330, 781, 791, 1110, 1380, 1425
Pfl23II CGTACG 1 cut(s) 881
PkrI GCNGC 7 cut(s) 187, 217, 255, 625, 681, 1234, 1252
PleI GAGTC 1 cut(s) 282
PluTI GGCGCC 2 cut(s) 186, 1291
PpsI GAGTC 1 cut(s) 282
Psp1406I AACGTT 1 cut(s) 78
Psp6I CCWGG 1 cut(s) 1290
PspGI CCWGG 1 cut(s) 1290
PspLI CGTACG 1 cut(s) 881
PspN4I GGNNCC 6 cut(s) 103, 184, 222, 454, 995, 1289
PspPI GGNCC 1 cut(s) 965
PsuI RGATCY 2 cut(s) 58, 544
PvuII CAGCTG 1 cut(s) 1226
RsaI GTAC 6 cut(s) 11, 29, 776, 834, 883, 1341
RsaNI GTAC 6 cut(s) 10, 28, 775, 833, 882, 1340
RseI CAYNNNNRTG 1 cut(s) 572
SaqAI TTAA 8 cut(s) 21, 81, 345, 368, 948, 1092, 1169, 1185
SatI GCNGC 7 cut(s) 186, 216, 254, 624, 680, 1233, 1251
Sau3AI GATC 8 cut(s) 58, 544, 674, 695, 848, 877, 1009, 1096
Sau96I GGNCC 1 cut(s) 965
SchI GAGTC 1 cut(s) 282
ScrFI CCNGG 2 cut(s) 1103, 1292
SduI GDGCHC 1 cut(s) 1077
SfaNI GCATC 2 cut(s) 448, 970
SfcI CTRYAG 2 cut(s) 593, 1282
SfoI GGCGCC 2 cut(s) 184, 1289
SfuI TTCGAA 1 cut(s) 236
SgrAI CRCCGGYG 1 cut(s) 170
SmiMI CAYNNNNRTG 1 cut(s) 572
SphI GCATGC 2 cut(s) 1180, 1325
Sse9I AATT 6 cut(s) 342, 600, 701, 1170, 1239, 1254
SseBI AGGCCT 2 cut(s) 507, 1155
SsiI CCGC 6 cut(s) 108, 186, 218, 450, 522, 707
SspDI GGCGCC 2 cut(s) 182, 1287
SspI AATATT 1 cut(s) 199
SspMI CTAG 1 cut(s) 497
StuI AGGCCT 2 cut(s) 507, 1155
StyD4I CCNGG 2 cut(s) 1101, 1290
StyI CCWWGG 1 cut(s) 356
TaaI ACNGT 2 cut(s) 295, 444
TaiI ACGT 4 cut(s) 49, 81, 279, 324
TaqI TCGA 5 cut(s) 236, 694, 789, 1164, 1269
TasI AATT 6 cut(s) 342, 600, 701, 1170, 1239, 1254
TatI WGTACW 1 cut(s) 27
TauI GCSGC 1 cut(s) 188
TfiI GAWTC 6 cut(s) 330, 781, 791, 1110, 1380, 1425
Tru1I TTAA 8 cut(s) 21, 81, 345, 368, 948, 1092, 1169, 1185
Tru9I TTAA 8 cut(s) 21, 81, 345, 368, 948, 1092, 1169, 1185
TscAI CASTG 2 cut(s) 1124, 1332
TseFI GTSAC 1 cut(s) 839
TseI GCWGC 6 cut(s) 215, 253, 623, 679, 1232, 1250
Tsp45I GTSAC 1 cut(s) 839
TspDTI ATGAA 2 cut(s) 54, 1119
TspGWI ACGGA 4 cut(s) 222, 496, 765, 947
TspRI CASTG 2 cut(s) 1124, 1332
XapI RAATTY 1 cut(s) 1239
XceI RCATGY 2 cut(s) 1180, 1325
XmiI GTMKAC 1 cut(s) 921
XmnI GAANNNNTTC 1 cut(s) 1020
XspI CTAG 1 cut(s) 497
ZraI GACGTC 1 cut(s) 277
Zsp2I ATGCAT 2 cut(s) 1182, 1323
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.