pycom16g12340

Methyltransferase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0012435
Physical Location & Seq
Forward (+)
8663590 .. 8665189
1600 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 744 bp
ATGACGATGGGAACGACGACGCAGGCGTACGGCGAGTCGCGGTACTGGGACAACCGCTACGCCAACGAATCAGGATCGTTCGATTGGTACCAAAAGTACCAATCTTTGGCTCCGCTGATCAATCTCTACGTCCCTCGCCATCCCAACCAACACAACCGCATCCTTGTCGTCGGCTGCGGCAACTCAGCGTTCAGCGAGGGGATGGTTGATGATGGGTACGAGGATGTGGTTAGTATTGACATTTCATCTGTGGTCGTCCAAACTATGCAGAGCAAGTACTCGAATCGTCCACACCTCAAATATTTGCAAATGGATGTTCGAGATATGAGTGCTTTCGAAACTGCTTCCTTTGATGCTGTTGTTGACAAAGGAACTCTAGATTCTCTTTTGTGTGGAAGTAATTCGCGGCAAAATGCTACCGATATGCTTGAGGAAGTTTGGAGGGTCCTCAAGGATAAAGGAGTCTACTTTCTGGTCACATATGGTGCCCCATTGTATCGTTTGCGCTTGTTGAGAGAGTCATGCTCGTGGATGATAAAACTCCATGTGATAGGGAAACTTTCCCGTGAAGATAAACCAGAACCTCCAATATGGGATCTGACAAATCCTGTACCGCTGCATGGTGATGGAAGAAGCTCAGCGGAGGAATTGCTAGGAAACAACCCTGATGTTCATTATATTTACATTTGTACCAAGGATAATTCTTTAAAGCCAGGCGTTAAGCGTGAACCCTCGGTCGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_11

Protein Analysis

248

Amino Acids

28.06

Weight (kDa)

5.54

Isoelectric Point (pI)

36.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPMT PF05724 14 - 160 3e-07 Thiopurine S-methyltransferase (TPMT)
Methyltransf_31 PF13847 51 - 162 2.6e-10 Methyltransferase domain
Methyltransf_25 PF13649 54 - 154 7.6e-12 Methyltransferase domain
Methyltransf_11 PF08241 55 - 157 1e-14 Methyltransferase domain
Methyltransf_12 PF08242 55 - 155 6.4e-07 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012435)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G17365 AT3G17365
fragaria_vesca FvH4_4g21180 FvH4_4g21180
malus_domestica MD13G1144200.v1.1
prunus_persica Prupe.1G108600_v2.0.a1 Prupe.1G108600_v2.0.a1
pyrus_communis pycom16g12340
rosa_chinensis RchiOBHm_Chr4g0426751
rosa_laevigata RLG00000007280
rosa_multiflora Rmu_co8157368.1_g000001 Rmu_sc0000953.1_g000002
rosa_roxburghii Rroxscaffold_5G00368750
rosa_rugosa Rorug04G0214600
rosa_samantha Rh4AG269700 Rh4BG276000 Rh4CG290600 Rh4DG273000
rosa_wichuraiana Rw4G023400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 87
AccB1I GGYRCC 2 cut(s) 87, 485
AccB7I CCANNNNNTGG 1 cut(s) 106
AccI GTMKAC 1 cut(s) 465
AccII CGCG 2 cut(s) 40, 406
AciI CCGC 8 cut(s) 40, 55, 113, 157, 177, 406, 614, 641
AclWI GGATC 2 cut(s) 82, 603
AfaI GTAC 8 cut(s) 29, 44, 89, 98, 218, 278, 612, 691
AfiI CCNNNNNNNGG 2 cut(s) 106, 620
AjnI CCWGG 1 cut(s) 712
AluBI AGCT 1 cut(s) 636
AluI AGCT 1 cut(s) 636
AlwI GGATC 2 cut(s) 82, 603
ApeKI GCWGC 2 cut(s) 174, 616
Asp700I GAANNNNTTC 1 cut(s) 400
Asp718I GGTACC 1 cut(s) 87
AspLEI GCGC 1 cut(s) 507
AspS9I GGNCC 1 cut(s) 445
AsuHPI GGTGA 1 cut(s) 635
AsuII TTCGAA 1 cut(s) 336
AvaII GGWCC 1 cut(s) 445
BaeGI GKGCMC 1 cut(s) 490
BaeI ACNNNNGTAYC 2 cut(s) 673, 706
BanI GGYRCC 2 cut(s) 87, 485
BauI CACGAG 1 cut(s) 526
BbvI GCAGC 2 cut(s) 161, 603
BccI CCATC 4 cut(s) 147, 196, 206, 620
BceAI ACGGC 1 cut(s) 46
BcgI CGANNNNNNTGC 2 cut(s) 148, 182
BciT130I CCWGG 1 cut(s) 714
BclI TGATCA 1 cut(s) 117
BfaI CTAG 2 cut(s) 377, 653
BisI GCNGC 4 cut(s) 175, 178, 407, 617
BlpI GCTNAGC 1 cut(s) 637
BlsI GCNGC 4 cut(s) 176, 179, 408, 618
BmcAI AGTACT 1 cut(s) 278
Bme1390I CCNGG 1 cut(s) 714
Bme18I GGWCC 1 cut(s) 445
BmgT120I GGNCC 1 cut(s) 445
BmiI GGNNCC 4 cut(s) 89, 111, 446, 487
BmrFI CCNGG 1 cut(s) 714
BmrI ACTGGG 1 cut(s) 55
BmsI GCATC 2 cut(s) 168, 343
BmuI ACTGGG 1 cut(s) 55
BplI GAGNNNNNCTC 2 cut(s) 509, 541
Bpu1102I GCTNAGC 1 cut(s) 637
Bpu14I TTCGAA 1 cut(s) 336
BpuEI CTTGAG 2 cut(s) 434, 449
BsaJI CCNNGG 2 cut(s) 693, 732
Bsc4I CCNNNNNNNGG 2 cut(s) 106, 620
Bse1I ACTGG 1 cut(s) 50
BseBI CCWGG 1 cut(s) 714
BseDI CCNNGG 2 cut(s) 693, 732
BseGI GGATG 6 cut(s) 139, 159, 207, 229, 319, 537
BseLI CCNNNNNNNGG 2 cut(s) 106, 620
BseMII CTCAG 2 cut(s) 198, 651
BseNI ACTGG 1 cut(s) 50
BseSI GKGCMC 1 cut(s) 490
BseXI GCAGC 2 cut(s) 161, 603
Bsh1236I CGCG 2 cut(s) 40, 406
Bsh1285I CGRYCG 1 cut(s) 738
BshNI GGYRCC 2 cut(s) 87, 485
BsiEI CGRYCG 1 cut(s) 738
BsiWI CGTACG 1 cut(s) 27
BslFI GGGAC 2 cut(s) 62, 116
BslI CCNNNNNNNGG 2 cut(s) 106, 620
BsmFI GGGAC 2 cut(s) 62, 116
Bsp119I TTCGAA 1 cut(s) 336
Bsp1286I GDGCHC 1 cut(s) 490
Bsp143I GATC 3 cut(s) 74, 117, 595
Bsp1720I GCTNAGC 1 cut(s) 637
BspACI CCGC 8 cut(s) 40, 55, 113, 157, 177, 406, 614, 641
BspCNI CTCAG 2 cut(s) 197, 650
BspFNI CGCG 2 cut(s) 40, 406
BspLI GGNNCC 4 cut(s) 89, 111, 446, 487
BspPI GGATC 2 cut(s) 82, 603
BspT104I TTCGAA 1 cut(s) 336
BspT107I GGYRCC 2 cut(s) 87, 485
BsrI ACTGG 1 cut(s) 50
BssECI CCNNGG 2 cut(s) 693, 732
BssMI GATC 3 cut(s) 74, 117, 595
BssSI CACGAG 1 cut(s) 526
BssT1I CCWWGG 1 cut(s) 693
Bst2BI CACGAG 1 cut(s) 526
Bst2UI CCWGG 1 cut(s) 714
BstBI TTCGAA 1 cut(s) 336
BstC8I GCNNGC 1 cut(s) 24
BstDEI CTNAG 2 cut(s) 184, 637
BstF5I GGATG 6 cut(s) 139, 159, 207, 229, 319, 537
BstFNI CGCG 2 cut(s) 40, 406
BstHHI GCGC 1 cut(s) 507
BstKTI GATC 3 cut(s) 77, 120, 598
BstMBI GATC 3 cut(s) 74, 117, 595
BstMCI CGRYCG 1 cut(s) 738
BstNI CCWGG 1 cut(s) 714
BstSCI CCNGG 1 cut(s) 712
BstSLI GKGCMC 1 cut(s) 490
BstUI CGCG 2 cut(s) 40, 406
BstV1I GCAGC 2 cut(s) 161, 603
BstX2I RGATCY 1 cut(s) 595
BstYI RGATCY 1 cut(s) 595
BtsCI GGATG 6 cut(s) 139, 159, 207, 229, 319, 537
Cac8I GCNNGC 1 cut(s) 24
CfoI GCGC 1 cut(s) 507
Cfr13I GGNCC 1 cut(s) 445
CseI GACGC 1 cut(s) 28
Csp6I GTAC 8 cut(s) 28, 43, 88, 97, 217, 277, 611, 690
CviAII CATG 3 cut(s) 522, 545, 620
CviJI RGCY 4 cut(s) 110, 174, 636, 712
CviKI_1 RGCY 4 cut(s) 110, 174, 636, 712
CviQI GTAC 8 cut(s) 28, 43, 88, 97, 217, 277, 611, 690
DdeI CTNAG 2 cut(s) 184, 637
DpnI GATC 3 cut(s) 76, 119, 597
DpnII GATC 3 cut(s) 74, 117, 595
DraI TTTAAA 1 cut(s) 708
Eco130I CCWWGG 1 cut(s) 693
Eco47I GGWCC 1 cut(s) 445
EcoO109I RGGNCCY 1 cut(s) 445
EcoRII CCWGG 1 cut(s) 712
EcoT14I CCWWGG 1 cut(s) 693
ErhI CCWWGG 1 cut(s) 693
FaeI CATG 3 cut(s) 525, 548, 623
FaqI GGGAC 2 cut(s) 62, 116
FatI CATG 3 cut(s) 521, 544, 619
FauNDI CATATG 1 cut(s) 481
FbaI TGATCA 1 cut(s) 117
FblI GTMKAC 1 cut(s) 465
Fnu4HI GCNGC 4 cut(s) 175, 178, 407, 617
FokI GGATG 6 cut(s) 126, 146, 214, 236, 326, 544
Fsp4HI GCNGC 4 cut(s) 175, 178, 407, 617
FspBI CTAG 2 cut(s) 377, 653
GlaI GCGC 1 cut(s) 506
GluI GCNGC 4 cut(s) 175, 178, 407, 617
HgaI GACGC 1 cut(s) 28
HhaI GCGC 1 cut(s) 507
Hin1II CATG 3 cut(s) 525, 548, 623
Hin6I GCGC 1 cut(s) 505
HinP1I GCGC 1 cut(s) 505
HincII GTYRAC 1 cut(s) 364
HindII GTYRAC 1 cut(s) 364
HinfI GANTC 6 cut(s) 35, 68, 283, 380, 462, 518
HphI GGTGA 1 cut(s) 635
Hpy166II GTNNAC 4 cut(s) 290, 364, 466, 728
Hpy188I TCNGA 1 cut(s) 600
Hpy188III TCNNGA 3 cut(s) 72, 320, 377
Hpy8I GTNNAC 4 cut(s) 290, 364, 466, 728
Hpy99I CGWCG 3 cut(s) 19, 22, 173
HpyCH4IV ACGT 1 cut(s) 129
HpyCH4V TGCA 3 cut(s) 268, 307, 619
HpyF3I CTNAG 2 cut(s) 184, 637
HpySE526I ACGT 1 cut(s) 129
Hsp92II CATG 3 cut(s) 525, 548, 623
HspAI GCGC 1 cut(s) 505
KpnI GGTACC 1 cut(s) 91
Ksp22I TGATCA 1 cut(s) 117
Kzo9I GATC 3 cut(s) 74, 117, 595
LmnI GCTCC 1 cut(s) 115
LpnPI CCDG 9 cut(s) 8, 31, 57, 458, 591, 621, 678, 699, 726
Lsp1109I GCAGC 2 cut(s) 161, 603
LweI GCATC 2 cut(s) 168, 343
MaeI CTAG 2 cut(s) 377, 653
MaeII ACGT 1 cut(s) 129
MaeIII GTNAC 1 cut(s) 475
MalI GATC 3 cut(s) 76, 119, 597
MboI GATC 3 cut(s) 74, 117, 595
MboII GAAGA 2 cut(s) 581, 642
MflI RGATCY 1 cut(s) 595
MhlI GDGCHC 1 cut(s) 490
MluCI AATT 3 cut(s) 400, 647, 700
MlyI GAGTC 3 cut(s) 44, 471, 527
MroXI GAANNNNTTC 1 cut(s) 400
MseI TTAA 2 cut(s) 707, 720
MslI CAYNNNNRTG 2 cut(s) 526, 624
MspA1I CMGCKG 3 cut(s) 115, 616, 641
MspR9I CCNGG 1 cut(s) 714
MvaI CCWGG 1 cut(s) 714
MvnI CGCG 2 cut(s) 40, 406
NdeI CATATG 1 cut(s) 481
NdeII GATC 3 cut(s) 74, 117, 595
NlaIII CATG 3 cut(s) 525, 548, 623
NlaIV GGNNCC 4 cut(s) 89, 111, 446, 487
NmuCI GTSAC 1 cut(s) 475
NspV TTCGAA 1 cut(s) 336
PcsI WCGNNNNNNNCGW 2 cut(s) 11, 23
PdmI GAANNNNTTC 1 cut(s) 400
PfeI GAWTC 3 cut(s) 68, 283, 380
Pfl23II CGTACG 1 cut(s) 27
PflMI CCANNNNNTGG 1 cut(s) 106
PkrI GCNGC 4 cut(s) 176, 179, 408, 618
PleI GAGTC 3 cut(s) 43, 470, 526
PpsI GAGTC 3 cut(s) 43, 470, 526
PpuMI RGGWCCY 1 cut(s) 445
Psp5II RGGWCCY 1 cut(s) 445
Psp6I CCWGG 1 cut(s) 712
PspGI CCWGG 1 cut(s) 712
PspLI CGTACG 1 cut(s) 27
PspN4I GGNNCC 4 cut(s) 89, 111, 446, 487
PspPI GGNCC 1 cut(s) 445
PspPPI RGGWCCY 1 cut(s) 445
PsuI RGATCY 1 cut(s) 595
RsaI GTAC 8 cut(s) 29, 44, 89, 98, 218, 278, 612, 691
RsaNI GTAC 8 cut(s) 28, 43, 88, 97, 217, 277, 611, 690
RseI CAYNNNNRTG 2 cut(s) 526, 624
SaqAI TTAA 2 cut(s) 707, 720
SatI GCNGC 4 cut(s) 175, 178, 407, 617
Sau3AI GATC 3 cut(s) 74, 117, 595
Sau96I GGNCC 1 cut(s) 445
ScaI AGTACT 1 cut(s) 278
SchI GAGTC 3 cut(s) 44, 471, 527
ScrFI CCNGG 1 cut(s) 714
SduI GDGCHC 1 cut(s) 490
SetI ASST 4 cut(s) 132, 297, 586, 638
SfaNI GCATC 2 cut(s) 168, 343
SfuI TTCGAA 1 cut(s) 336
SinI GGWCC 1 cut(s) 445
SmiMI CAYNNNNRTG 2 cut(s) 526, 624
SmlI CTYRAG 2 cut(s) 428, 449
SmoI CTYRAG 2 cut(s) 428, 449
Sse9I AATT 3 cut(s) 400, 647, 700
SsiI CCGC 8 cut(s) 40, 55, 113, 157, 177, 406, 614, 641
SspI AATATT 1 cut(s) 302
SspMI CTAG 2 cut(s) 377, 653
StyD4I CCNGG 1 cut(s) 712
StyI CCWWGG 1 cut(s) 693
TaiI ACGT 1 cut(s) 132
TaqI TCGA 5 cut(s) 81, 281, 319, 336, 738
TaqII GACCGA 1 cut(s) 724
TasI AATT 3 cut(s) 400, 647, 700
TatI WGTACW 1 cut(s) 276
TauI GCSGC 2 cut(s) 180, 409
TfiI GAWTC 3 cut(s) 68, 283, 380
Tru1I TTAA 2 cut(s) 707, 720
Tru9I TTAA 2 cut(s) 707, 720
TseFI GTSAC 1 cut(s) 475
TseI GCWGC 2 cut(s) 174, 616
Tsp45I GTSAC 1 cut(s) 475
TspDTI ATGAA 2 cut(s) 234, 662
Van91I CCANNNNNTGG 1 cut(s) 106
VpaK11BI GGWCC 1 cut(s) 445
XbaI TCTAGA 1 cut(s) 376
XmiI GTMKAC 1 cut(s) 465
XmnI GAANNNNTTC 1 cut(s) 400
XspI CTAG 2 cut(s) 377, 653
ZrmI AGTACT 1 cut(s) 278
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.