pycom16g12770

Cleavage inducing molecular chaperone

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0003374
Physical Location & Seq
Forward (+)
9043797 .. 9044982
1186 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 486 bp
ATGGCTTCTAATGGAGGGCAGGGTGTCTTTCCCCCTGGATTTGCACACCCGAAAGCCGATGGTGAAGACCCATTTGGGGATTCAAGGAGAATCGCCTGCAATAAGTGCGGCAATTTTCATGTCTGGGTGCTCACCAGGAAATCAAAATCTCAAGCACGGTGGTGCCAGGGAATGAGATGTCCAGCCAACACTCACAAGCCAAGTTTTCATGTTAACATTAGTGTGACCTCTAAGCATAATACTGGAAAGGGAACAAGTTCGGGGCAAAGAGGAGGTCAGATGCCAACATCGAACTTGGAAGAGAACATGACAGAGGAAGAATTCTTCGAGTGGTTACAGAATGCAGTGCAAACTGGCATGTTTGAAAATTTTGGTACCGGCACCTCCACTGAGAGCCCATCTGCCAAATCTGGACCTGGTACCAAGAGTGGTGGCAGCACCAGTGGCAGTGCTAACAAGAGAAAGAAAAAGGGAAAGAAATGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DnaJ Jiv90

Protein Analysis

162

Amino Acids

17.33

Weight (kDa)

9.77

Isoelectric Point (pI)

49.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Jiv90 PF14901 28 - 56 3.6e-08 Cleavage inducing molecular chaperone
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 374, 419
AccB1I GGYRCC 4 cut(s) 162, 374, 380, 419
AciI CCGC 1 cut(s) 108
AcsI RAATTY 2 cut(s) 320, 367
AfaI GTAC 2 cut(s) 376, 421
AfiI CCNNNNNNNGG 1 cut(s) 76
AgsI TTSAA 2 cut(s) 84, 365
AjnI CCWGG 4 cut(s) 34, 134, 165, 415
AjuI GAANNNNNNNTTGG 2 cut(s) 57, 89
AleI CACNNNNGTG 1 cut(s) 160
Alw21I GWGCWC 1 cut(s) 132
ApeKI GCWGC 1 cut(s) 435
ApoI RAATTY 2 cut(s) 320, 367
ArsI GACNNNNNNTTYG 2 cut(s) 259, 291
Asp700I GAANNNNTTC 1 cut(s) 256
Asp718I GGTACC 2 cut(s) 374, 419
AspS9I GGNCC 1 cut(s) 413
AsuHPI GGTGA 2 cut(s) 74, 124
AvaII GGWCC 1 cut(s) 413
BanI GGYRCC 4 cut(s) 162, 374, 380, 419
BanII GRGCYC 1 cut(s) 398
BbsI GAAGAC 1 cut(s) 72
Bbv12I GWGCWC 1 cut(s) 132
BbvI GCAGC 1 cut(s) 447
BccI CCATC 2 cut(s) 53, 406
BciT130I CCWGG 4 cut(s) 36, 136, 167, 417
BisI GCNGC 2 cut(s) 109, 436
BlsI GCNGC 2 cut(s) 110, 437
Bme1390I CCNGG 4 cut(s) 36, 136, 167, 417
Bme18I GGWCC 1 cut(s) 413
BmgT120I GGNCC 1 cut(s) 413
BmiI GGNNCC 4 cut(s) 164, 376, 382, 421
BmrFI CCNGG 4 cut(s) 36, 136, 167, 417
BmsI GCATC 1 cut(s) 270
BpiI GAAGAC 1 cut(s) 72
BpuEI CTTGAG 1 cut(s) 135
BsaJI CCNNGG 2 cut(s) 34, 166
Bsc4I CCNNNNNNNGG 1 cut(s) 76
Bse118I RCCGGY 1 cut(s) 377
Bse1I ACTGG 3 cut(s) 247, 358, 441
BseBI CCWGG 4 cut(s) 36, 136, 167, 417
BseDI CCNNGG 2 cut(s) 34, 166
BseLI CCNNNNNNNGG 1 cut(s) 76
BseMII CTCAG 1 cut(s) 381
BseNI ACTGG 3 cut(s) 247, 358, 441
BseRI GAGGAG 1 cut(s) 285
BseXI GCAGC 1 cut(s) 447
BshNI GGYRCC 4 cut(s) 162, 374, 380, 419
BsiHKAI GWGCWC 1 cut(s) 132
BsiSI CCGG 1 cut(s) 378
BslI CCNNNNNNNGG 1 cut(s) 76
BsmI GAATGC 1 cut(s) 346
Bsp1286I GDGCHC 2 cut(s) 132, 398
BspACI CCGC 1 cut(s) 108
BspCNI CTCAG 1 cut(s) 382
BspLI GGNNCC 4 cut(s) 164, 376, 382, 421
BspT107I GGYRCC 4 cut(s) 162, 374, 380, 419
BsrFI RCCGGY 1 cut(s) 377
BsrI ACTGG 3 cut(s) 247, 358, 441
BssAI RCCGGY 1 cut(s) 377
BssECI CCNNGG 2 cut(s) 34, 166
Bst2UI CCWGG 4 cut(s) 36, 136, 167, 417
Bst4CI ACNGT 1 cut(s) 159
Bst6I CTCTTC 1 cut(s) 294
BstAPI GCANNNNNTGC 1 cut(s) 105
BstC8I GCNNGC 1 cut(s) 97
BstDEI CTNAG 2 cut(s) 231, 390
BstMWI GCNNNNNNNGC 2 cut(s) 105, 444
BstNI CCWGG 4 cut(s) 36, 136, 167, 417
BstNSI RCATGY 1 cut(s) 361
BstSCI CCNGG 4 cut(s) 34, 134, 165, 415
BstV1I GCAGC 1 cut(s) 447
BstV2I GAAGAC 1 cut(s) 72
BtsI GCAGTG 2 cut(s) 351, 454
BtsIMutI CAGTG 4 cut(s) 351, 387, 448, 454
Cac8I GCNNGC 1 cut(s) 97
Cfr10I RCCGGY 1 cut(s) 377
Cfr13I GGNCC 1 cut(s) 413
CsiI ACCWGGT 1 cut(s) 415
Csp6I GTAC 2 cut(s) 375, 420
CspCI CAANNNNNGTGG 4 cut(s) 140, 175, 412, 447
CviAII CATG 4 cut(s) 119, 209, 307, 358
CviJI RGCY 5 cut(s) 5, 56, 185, 199, 396
CviKI_1 RGCY 5 cut(s) 5, 56, 185, 199, 396
CviQI GTAC 2 cut(s) 375, 420
DdeI CTNAG 2 cut(s) 231, 390
Eam1104I CTCTTC 1 cut(s) 294
EarI CTCTTC 1 cut(s) 294
Eco24I GRGCYC 1 cut(s) 398
Eco47I GGWCC 1 cut(s) 413
EcoRI GAATTC 1 cut(s) 320
EcoRII CCWGG 4 cut(s) 34, 134, 165, 415
EcoT38I GRGCYC 1 cut(s) 398
FaeI CATG 4 cut(s) 122, 212, 310, 361
FaiI YATR 5 cut(s) 120, 210, 237, 308, 359
FatI CATG 4 cut(s) 118, 208, 306, 357
Fnu4HI GCNGC 2 cut(s) 109, 436
FriOI GRGCYC 1 cut(s) 398
Fsp4HI GCNGC 2 cut(s) 109, 436
GluI GCNGC 2 cut(s) 109, 436
HapII CCGG 1 cut(s) 378
Hin1II CATG 4 cut(s) 122, 212, 310, 361
HincII GTYRAC 1 cut(s) 214
HindII GTYRAC 1 cut(s) 214
HinfI GANTC 2 cut(s) 80, 90
HpaI GTTAAC 1 cut(s) 214
HpaII CCGG 1 cut(s) 378
HphI GGTGA 2 cut(s) 74, 124
Hpy166II GTNNAC 1 cut(s) 214
Hpy188I TCNGA 1 cut(s) 279
Hpy188III TCNNGA 1 cut(s) 411
Hpy8I GTNNAC 1 cut(s) 214
HpyCH4III ACNGT 1 cut(s) 159
HpyCH4V TGCA 4 cut(s) 44, 99, 344, 349
HpyF10VI GCNNNNNNNGC 2 cut(s) 105, 444
HpyF3I CTNAG 2 cut(s) 231, 390
Hsp92II CATG 4 cut(s) 122, 212, 310, 361
KpnI GGTACC 2 cut(s) 378, 423
KspAI GTTAAC 1 cut(s) 214
Lsp1109I GCAGC 1 cut(s) 447
LweI GCATC 1 cut(s) 270
MabI ACCWGGT 1 cut(s) 415
MaeIII GTNAC 2 cut(s) 223, 333
MboII GAAGA 4 cut(s) 77, 311, 316, 329
MhlI GDGCHC 2 cut(s) 132, 398
MluCI AATT 3 cut(s) 112, 320, 367
MnlI CCTC 6 cut(s) 8, 238, 263, 266, 307, 394
MroXI GAANNNNTTC 1 cut(s) 256
MseI TTAA 1 cut(s) 213
MslI CAYNNNNRTG 2 cut(s) 160, 221
MspI CCGG 1 cut(s) 378
MspR9I CCNGG 4 cut(s) 36, 136, 167, 417
Mva1269I GAATGC 1 cut(s) 346
MvaI CCWGG 4 cut(s) 36, 136, 167, 417
MwoI GCNNNNNNNGC 2 cut(s) 105, 444
NlaIII CATG 4 cut(s) 122, 212, 310, 361
NlaIV GGNNCC 4 cut(s) 164, 376, 382, 421
NmuCI GTSAC 1 cut(s) 223
NspI RCATGY 1 cut(s) 361
OliI CACNNNNGTG 1 cut(s) 160
PctI GAATGC 1 cut(s) 346
PdmI GAANNNNTTC 1 cut(s) 256
PfeI GAWTC 2 cut(s) 80, 90
PkrI GCNGC 2 cut(s) 110, 437
Psp6I CCWGG 4 cut(s) 34, 134, 165, 415
PspGI CCWGG 4 cut(s) 34, 134, 165, 415
PspN4I GGNNCC 4 cut(s) 164, 376, 382, 421
PspPI GGNCC 1 cut(s) 413
RsaI GTAC 2 cut(s) 376, 421
RsaNI GTAC 2 cut(s) 375, 420
RseI CAYNNNNRTG 2 cut(s) 160, 221
SaqAI TTAA 1 cut(s) 213
SatI GCNGC 2 cut(s) 109, 436
Sau96I GGNCC 1 cut(s) 413
ScrFI CCNGG 4 cut(s) 36, 136, 167, 417
SduI GDGCHC 2 cut(s) 132, 398
SetI ASST 4 cut(s) 230, 277, 386, 418
SexAI ACCWGGT 1 cut(s) 415
SfaNI GCATC 1 cut(s) 270
SinI GGWCC 1 cut(s) 413
SmiMI CAYNNNNRTG 2 cut(s) 160, 221
SmlI CTYRAG 1 cut(s) 150
SmoI CTYRAG 1 cut(s) 150
Sse9I AATT 3 cut(s) 112, 320, 367
SsiI CCGC 1 cut(s) 108
StyD4I CCNGG 4 cut(s) 34, 134, 165, 415
TaaI ACNGT 1 cut(s) 159
TaqI TCGA 2 cut(s) 290, 327
TasI AATT 3 cut(s) 112, 320, 367
TauI GCSGC 1 cut(s) 111
TfiI GAWTC 2 cut(s) 80, 90
Tru1I TTAA 1 cut(s) 213
Tru9I TTAA 1 cut(s) 213
TscAI CASTG 4 cut(s) 351, 394, 448, 454
TseFI GTSAC 1 cut(s) 223
TseI GCWGC 1 cut(s) 435
Tsp45I GTSAC 1 cut(s) 223
TspDTI ATGAA 2 cut(s) 107, 197
TspRI CASTG 4 cut(s) 351, 394, 448, 454
VpaK11BI GGWCC 1 cut(s) 413
XapI RAATTY 2 cut(s) 320, 367
XceI RCATGY 1 cut(s) 361
XmnI GAANNNNTTC 1 cut(s) 256
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.