pycom16g12950

The GINS complex plays an essential role in the initiation of DNA replication

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0006170
Physical Location & Seq
Forward (+)
9195687 .. 9197901
2215 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 654 bp
ATGGACTCGGGTTTGGGTTTGGATTCGGGTCTGGAATCAGGGTCAACGGAGGATTACGAGACGGACGTGGAGCTTCTGAAGAGGGCGTGGCGGCAGGAGAAGGCTGCTCCGGAGGTTCTTCAGTTCGAGAGTGCTTTGATTCGGAGAATCATTGAGCAGATCCAATTGATGGAAGAAACTGTGGAGGAGTTCACTGAGAGCGGTATTGATCCGCTTACCGTGTCACTGTACCAGATGGATTTGGACAGAACTCAGTTTTTGTTGAGATCTTATTACCGAATTCGTTTGCAGAAGATTGAGAAATACATGTTTCACATTCGGGATAATGCTGAAAGCCGTCTCTCTGATAAGGAGAAGCTGTTTGTTGAACGGTGCTGCGCTGATTTGCAGACGCATCTTGAAAAGAGCATTCTGTTGCAGTTGCCTTCTACTTATCAGTCTGTATTCCGTCAATCAAGTGAAAAGGAGACTGACATGGTGCCGAGACACCAAATGGATACATTTGTTGTCTGCAAAACCAAGTACTATCTTGGACATATCCAGCTCGAGGACGATGGAGAATCAGATAGCAGGACAGTGCGAGCCAGAGACCATTGGACGAGCCGTTTGAGATGGAGCCTAATGTGTTGTACTTTGTACGTTATAAAGCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SLD5_C Sld5

Protein Analysis

218

Amino Acids

25.64

Weight (kDa)

5.17

Isoelectric Point (pI)

65.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sld5 PF05916 71 - 133 4.1e-08 GINS complex protein helical bundle domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 644
AccB1I GGYRCC 1 cut(s) 478
AccB7I CCANNNNNTGG 1 cut(s) 169
AccBSI CCGCTC 1 cut(s) 201
AccIII TCCGGA 1 cut(s) 109
AciI CCGC 3 cut(s) 91, 201, 212
AclWI GGATC 2 cut(s) 154, 203
AcsI RAATTY 1 cut(s) 279
AcuI CTGAAG 2 cut(s) 98, 104
AfaI GTAC 4 cut(s) 230, 524, 631, 638
AfiI CCNNNNNNNGG 2 cut(s) 169, 547
AflIII ACRYGT 1 cut(s) 306
AgsI TTSAA 2 cut(s) 368, 401
AjiI CACGTC 1 cut(s) 67
AluBI AGCT 3 cut(s) 73, 358, 544
AluI AGCT 3 cut(s) 73, 358, 544
Alw26I GTCTC 5 cut(s) 53, 344, 461, 478, 582
AlwI GGATC 2 cut(s) 154, 203
Ama87I CYCGRG 2 cut(s) 7, 545
Aor13HI TCCGGA 1 cut(s) 109
ApeKI GCWGC 2 cut(s) 104, 375
ApoI RAATTY 1 cut(s) 279
ArsI GACNNNNNNTTYG 2 cut(s) 589, 621
AspLEI GCGC 1 cut(s) 380
AvaI CYCGRG 2 cut(s) 7, 545
BaeI ACNNNNGTAYC 2 cut(s) 489, 522
BanI GGYRCC 1 cut(s) 478
BbvI GCAGC 2 cut(s) 91, 362
BccI CCATC 4 cut(s) 163, 229, 548, 606
BceAI ACGGC 2 cut(s) 321, 588
BciVI GTATCC 1 cut(s) 490
BcoDI GTCTC 5 cut(s) 53, 344, 461, 478, 582
BfuI GTATCC 1 cut(s) 490
BglII AGATCT 1 cut(s) 266
BisI GCNGC 3 cut(s) 92, 105, 376
BlsI GCNGC 3 cut(s) 93, 106, 377
BmcAI AGTACT 1 cut(s) 524
BmeT110I CYCGRG 2 cut(s) 7, 545
BmgBI CACGTC 1 cut(s) 67
BmiI GGNNCC 2 cut(s) 480, 617
BmsI GCATC 1 cut(s) 403
BsaI GGTCTC 1 cut(s) 582
BsaWI WCCGGW 1 cut(s) 109
BsaXI ACNNNNNCTCC 2 cut(s) 344, 374
Bsc4I CCNNNNNNNGG 2 cut(s) 169, 547
BseAI TCCGGA 1 cut(s) 109
BseLI CCNNNNNNNGG 2 cut(s) 169, 547
BseMII CTCAG 2 cut(s) 186, 266
BseRI GAGGAG 1 cut(s) 200
BseXI GCAGC 2 cut(s) 91, 362
BshNI GGYRCC 1 cut(s) 478
BsiHKCI CYCGRG 2 cut(s) 7, 545
BsiSI CCGG 1 cut(s) 110
BslI CCNNNNNNNGG 2 cut(s) 169, 547
BsmAI GTCTC 5 cut(s) 53, 344, 461, 478, 582
BsmBI CGTCTC 2 cut(s) 53, 344
BsmI GAATGC 1 cut(s) 408
Bso31I GGTCTC 1 cut(s) 582
BsoBI CYCGRG 2 cut(s) 7, 545
Bsp13I TCCGGA 1 cut(s) 109
Bsp143I GATC 3 cut(s) 159, 208, 266
BspACI CCGC 3 cut(s) 91, 201, 212
BspCNI CTCAG 2 cut(s) 187, 265
BspEI TCCGGA 1 cut(s) 109
BspLI GGNNCC 2 cut(s) 480, 617
BspPI GGATC 2 cut(s) 154, 203
BspT107I GGYRCC 1 cut(s) 478
BspTNI GGTCTC 1 cut(s) 582
BsrBI CCGCTC 1 cut(s) 201
BssMI GATC 3 cut(s) 159, 208, 266
Bst4CI ACNGT 5 cut(s) 181, 220, 228, 372, 577
Bst6I CTCTTC 1 cut(s) 74
BstC8I GCNNGC 1 cut(s) 582
BstDEI CTNAG 2 cut(s) 195, 252
BstHHI GCGC 1 cut(s) 380
BstKTI GATC 3 cut(s) 162, 211, 269
BstMAI GTCTC 5 cut(s) 53, 344, 461, 478, 582
BstMBI GATC 3 cut(s) 159, 208, 266
BstNSI RCATGY 1 cut(s) 310
BstV1I GCAGC 2 cut(s) 91, 362
BstX2I RGATCY 2 cut(s) 159, 266
BstYI RGATCY 2 cut(s) 159, 266
BsuI GTATCC 1 cut(s) 490
BtrI CACGTC 1 cut(s) 67
BtsIMutI CAGTG 3 cut(s) 192, 224, 582
Cac8I GCNNGC 1 cut(s) 582
CfoI GCGC 1 cut(s) 380
CseI GACGC 1 cut(s) 400
Csp6I GTAC 4 cut(s) 229, 523, 630, 637
CviAII CATG 2 cut(s) 307, 475
CviJI RGCY 8 cut(s) 73, 104, 336, 358, 544, 584, 603, 618
CviKI_1 RGCY 8 cut(s) 73, 104, 336, 358, 544, 584, 603, 618
CviQI GTAC 4 cut(s) 229, 523, 630, 637
DdeI CTNAG 2 cut(s) 195, 252
DpnI GATC 3 cut(s) 161, 210, 268
DpnII GATC 3 cut(s) 159, 208, 266
Eam1104I CTCTTC 1 cut(s) 74
EarI CTCTTC 1 cut(s) 74
Eco31I GGTCTC 1 cut(s) 582
Eco57I CTGAAG 2 cut(s) 98, 104
Eco88I CYCGRG 2 cut(s) 7, 545
EcoRI GAATTC 1 cut(s) 279
Esp3I CGTCTC 2 cut(s) 53, 344
FaeI CATG 2 cut(s) 310, 478
FaiI YATR 4 cut(s) 308, 476, 537, 644
FatI CATG 2 cut(s) 306, 474
Fnu4HI GCNGC 3 cut(s) 92, 105, 376
Fsp4HI GCNGC 3 cut(s) 92, 105, 376
GlaI GCGC 1 cut(s) 379
GluI GCNGC 3 cut(s) 92, 105, 376
HapII CCGG 1 cut(s) 110
HgaI GACGC 1 cut(s) 400
HhaI GCGC 1 cut(s) 380
Hin1II CATG 2 cut(s) 310, 478
Hin6I GCGC 1 cut(s) 378
HinP1I GCGC 1 cut(s) 378
HincII GTYRAC 1 cut(s) 45
HindII GTYRAC 1 cut(s) 45
HinfI GANTC 6 cut(s) 5, 23, 35, 139, 147, 560
HpaII CCGG 1 cut(s) 110
Hpy166II GTNNAC 2 cut(s) 45, 192
Hpy188I TCNGA 4 cut(s) 78, 144, 346, 565
Hpy188III TCNNGA 5 cut(s) 32, 110, 127, 320, 398
Hpy8I GTNNAC 2 cut(s) 45, 192
HpyAV CCTTC 2 cut(s) 94, 435
HpyCH4III ACNGT 5 cut(s) 181, 220, 228, 372, 577
HpyCH4IV ACGT 2 cut(s) 66, 639
HpyCH4V TGCA 4 cut(s) 289, 388, 418, 513
HpyF3I CTNAG 2 cut(s) 195, 252
HpySE526I ACGT 2 cut(s) 66, 639
Hsp92II CATG 2 cut(s) 310, 478
HspAI GCGC 1 cut(s) 378
Kpn2I TCCGGA 1 cut(s) 109
Kzo9I GATC 3 cut(s) 159, 208, 266
LmnI GCTCC 3 cut(s) 70, 112, 615
LpnPI CCDG 8 cut(s) 17, 24, 80, 123, 245, 554, 556, 598
Lsp1109I GCAGC 2 cut(s) 91, 362
LweI GCATC 1 cut(s) 403
MaeII ACGT 2 cut(s) 66, 639
MaeIII GTNAC 1 cut(s) 222
MalI GATC 3 cut(s) 161, 210, 268
MbiI CCGCTC 1 cut(s) 201
MboI GATC 3 cut(s) 159, 208, 266
MboII GAAGA 4 cut(s) 91, 110, 185, 304
MfeI CAATTG 1 cut(s) 164
MflI RGATCY 2 cut(s) 159, 266
MluCI AATT 2 cut(s) 164, 279
MnlI CCTC 5 cut(s) 43, 75, 106, 178, 541
MroI TCCGGA 1 cut(s) 109
MspI CCGG 1 cut(s) 110
MunI CAATTG 1 cut(s) 164
Mva1269I GAATGC 1 cut(s) 408
NdeII GATC 3 cut(s) 159, 208, 266
NlaIII CATG 2 cut(s) 310, 478
NlaIV GGNNCC 2 cut(s) 480, 617
NmeAIII GCCGAG 1 cut(s) 507
NmuCI GTSAC 1 cut(s) 222
NspI RCATGY 1 cut(s) 310
PaeR7I CTCGAG 1 cut(s) 545
PciI ACATGT 1 cut(s) 306
PcsI WCGNNNNNNNCGW 1 cut(s) 63
PctI GAATGC 1 cut(s) 408
PfeI GAWTC 5 cut(s) 23, 35, 139, 147, 560
PflMI CCANNNNNTGG 1 cut(s) 169
PkrI GCNGC 3 cut(s) 93, 106, 377
PscI ACATGT 1 cut(s) 306
PsiI TTATAA 1 cut(s) 644
PspN4I GGNNCC 2 cut(s) 480, 617
PspXI VCTCGAGB 1 cut(s) 545
PsuI RGATCY 2 cut(s) 159, 266
RsaI GTAC 4 cut(s) 230, 524, 631, 638
RsaNI GTAC 4 cut(s) 229, 523, 630, 637
SatI GCNGC 3 cut(s) 92, 105, 376
Sau3AI GATC 3 cut(s) 159, 208, 266
ScaI AGTACT 1 cut(s) 524
SetI ASST 6 cut(s) 69, 75, 117, 360, 546, 642
SfaNI GCATC 1 cut(s) 403
Sfr274I CTCGAG 1 cut(s) 545
SlaI CTCGAG 1 cut(s) 545
SmlI CTYRAG 1 cut(s) 545
SmoI CTYRAG 1 cut(s) 545
Sse9I AATT 2 cut(s) 164, 279
SsiI CCGC 3 cut(s) 91, 201, 212
TaaI ACNGT 5 cut(s) 181, 220, 228, 372, 577
TaiI ACGT 2 cut(s) 69, 642
TaqI TCGA 2 cut(s) 126, 546
TasI AATT 2 cut(s) 164, 279
TatI WGTACW 2 cut(s) 522, 629
TauI GCSGC 1 cut(s) 94
TfiI GAWTC 5 cut(s) 23, 35, 139, 147, 560
TscAI CASTG 3 cut(s) 199, 231, 582
TseFI GTSAC 1 cut(s) 222
TseI GCWGC 2 cut(s) 104, 375
Tsp45I GTSAC 1 cut(s) 222
TspGWI ACGGA 3 cut(s) 62, 77, 437
TspRI CASTG 3 cut(s) 199, 231, 582
Van91I CCANNNNNTGG 1 cut(s) 169
XapI RAATTY 1 cut(s) 279
XceI RCATGY 1 cut(s) 310
XhoI CTCGAG 1 cut(s) 545
ZrmI AGTACT 1 cut(s) 524
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.