pycom16g12990

enoyl-CoA hydratase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0012967
Physical Location & Seq
Reverse (-)
9237477 .. 9238620
1144 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 804 bp
ATGGCCCGCCACCAATCGTCGCCGGAAAATCTCATACTTGTAAACCGCGAACCGACCGGAATCGCGTTCGTGACGATCAACCGTCCCAAGTCGCTCAACTCGCTGACCCGGCCCATGATGACGGACCTTGCCCAGGCCTTCAAGGCCCTAGATCAAGACGAATCGGTCCGGGTCATCGTTCTGTCTGGGTCCGGTCGCGCGTTTTGTTCCGGCGTCGATCTCACCTCCGCCGAGGACGTTTTCAAAGGCGATGTGAAGGACGTGCAGTCCGACCCCGTTTCCCAGATGGAGCGGTGCCGAAAGCCCATCATCGGAGCAATTAACGGGTTCGCGGTCACCGCCGGGTTCGAGATCGCGCTCGCCTGCGATATCATCATCGCGACGAAAGGAGCTAAATTCATGGACACTCACGCTAGATTTGGGATATTTCCGTCGTGGGGTTTGTCCCAGAAGCTTTCGAGGATCATAGGGCCCAACAAAGCGCGCGAAGTCTCGCTGGCGGCGACGCCGTTGACAGCGGAGGTGGGCGAAAAGTTGGGGTTCGTGAACCACGTTGTGGAAGAGAGTGAGTTGCTGAAGAAAGCTCGTGAAGTAGGTGAGGCCATTGCGAAAAATAACCATGACATGGTGTTGAGGTACAAGGCTGTTATCAATGACGGGCTTAAGCTGGACCTGGCCGACGCCCTTACGCTTGAAAAGGAGAGGGGTCATGAGTATTACAAGGGAATGACCAAAGCCCAGTTTAAGAAGATGCAAGAGTTCATAGCAGGTCGGAGTTCGAAAAAGCCTTCTTCCAAGTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ECH_1

Protein Analysis

268

Amino Acids

29.24

Weight (kDa)

9.15

Isoelectric Point (pI)

27.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ECH_1 PF00378 18 - 241 4.3e-53 Enoyl-CoA hydratase/isomerase
ECH_2 PF16113 22 - 203 4.9e-25 Enoyl-CoA hydratase/isomerase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012967)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G16210
fragaria_vesca FvH4_4g19880
malus_domestica MD16G1152800.v1.1
prunus_persica Prupe.1G116000_v2.0.a1
pyrus_communis pycom16g12990
rosa_chinensis RchiOBHm_Chr4g0425501 RchiOBHm_Chr4g0425511
rosa_laevigata RLG00000007359 RLG00000007363 RLG00000007365 RLG00000007367
rosa_multiflora Rmu_sc0001900.1_g000011
rosa_roxburghii Rroxscaffold_5G00367450
rosa_rugosa Rorug04G0203600
rosa_samantha Rh4AG260600 Rh4BG266300 Rh4DG262600
rosa_wichuraiana Rw4G022560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 164
Acc36I ACCTGC 1 cut(s) 758
AccB1I GGYRCC 1 cut(s) 294
AccB7I CCANNNNNTGG 2 cut(s) 556, 625
AccBSI CCGCTC 1 cut(s) 292
AccII CGCG 9 cut(s) 48, 65, 198, 200, 332, 356, 380, 484, 486
AciI CCGC 8 cut(s) 7, 46, 228, 292, 332, 339, 500, 518
AclWI GGATC 1 cut(s) 470
AcoI YGGCCR 1 cut(s) 675
AcsI RAATTY 1 cut(s) 395
AcuI CTGAAG 1 cut(s) 596
AcyI GRCGYC 3 cut(s) 213, 506, 681
AdeI CACNNNGTG 1 cut(s) 556
AfaI GTAC 1 cut(s) 638
AfiI CCNNNNNNNGG 5 cut(s) 133, 311, 437, 556, 625
AflII CTTAAG 1 cut(s) 662
AgsI TTSAA 3 cut(s) 142, 244, 695
AhdI GACNNNNNGTC 1 cut(s) 265
AjiI CACGTC 1 cut(s) 262
AjnI CCWGG 2 cut(s) 132, 672
AluBI AGCT 4 cut(s) 392, 454, 584, 667
AluI AGCT 4 cut(s) 392, 454, 584, 667
Alw26I GTCTC 1 cut(s) 496
AlwI GGATC 1 cut(s) 470
AoxI GGCC 7 cut(s) 3, 110, 135, 144, 470, 600, 675
ApaI GGGCCC 1 cut(s) 474
ApoI RAATTY 1 cut(s) 395
AspLEI GCGC 4 cut(s) 200, 358, 484, 486
AspS9I GGNCC 9 cut(s) 4, 111, 124, 145, 166, 189, 470, 471, 670
AsuC2I CCSGG 3 cut(s) 109, 170, 343
AsuHPI GGTGA 3 cut(s) 214, 328, 608
AsuII TTCGAA 1 cut(s) 779
AvaII GGWCC 4 cut(s) 124, 166, 189, 670
BaeGI GKGCMC 1 cut(s) 474
BanI GGYRCC 1 cut(s) 294
BanII GRGCYC 1 cut(s) 474
BauI CACGAG 1 cut(s) 585
BccI CCATC 2 cut(s) 280, 314
BceAI ACGGC 1 cut(s) 493
BciT130I CCWGG 2 cut(s) 134, 674
BcnI CCSGG 3 cut(s) 109, 170, 343
BcoDI GTCTC 1 cut(s) 496
BfaI CTAG 2 cut(s) 149, 414
BfrI CTTAAG 1 cut(s) 662
BfuAI ACCTGC 1 cut(s) 758
BglI GCCNNNNNGGC 1 cut(s) 143
BisI GCNGC 1 cut(s) 501
BlsI GCNGC 1 cut(s) 502
Bme1390I CCNGG 5 cut(s) 109, 134, 170, 343, 674
Bme18I GGWCC 4 cut(s) 124, 166, 189, 670
BmeRI GACNNNNNGTC 1 cut(s) 265
BmgBI CACGTC 1 cut(s) 262
BmgT120I GGNCC 9 cut(s) 4, 111, 124, 145, 166, 189, 470, 471, 670
BmiI GGNNCC 3 cut(s) 190, 296, 472
BmrFI CCNGG 5 cut(s) 109, 134, 170, 343, 674
BmrI ACTGGG 1 cut(s) 733
BmsI GCATC 1 cut(s) 741
BmuI ACTGGG 1 cut(s) 733
Bpu14I TTCGAA 1 cut(s) 779
BpuMI CCSGG 3 cut(s) 109, 170, 343
BsaHI GRCGYC 3 cut(s) 213, 506, 681
BsaJI CCNNGG 2 cut(s) 132, 231
BsaWI WCCGGW 2 cut(s) 56, 191
Bsc4I CCNNNNNNNGG 5 cut(s) 133, 311, 437, 556, 625
Bse1I ACTGG 1 cut(s) 739
Bse3DI GCAATG 1 cut(s) 603
BseBI CCWGG 2 cut(s) 134, 674
BseDI CCNNGG 2 cut(s) 132, 231
BseLI CCNNNNNNNGG 5 cut(s) 133, 311, 437, 556, 625
BseMI GCAATG 1 cut(s) 603
BseNI ACTGG 1 cut(s) 739
BsePI GCGCGC 1 cut(s) 482
BseSI GKGCMC 1 cut(s) 474
BsgI GTGCAG 1 cut(s) 284
Bsh1236I CGCG 9 cut(s) 48, 65, 198, 200, 332, 356, 380, 484, 486
Bsh1285I CGRYCG 2 cut(s) 57, 196
BshFI GGCC 7 cut(s) 5, 112, 137, 146, 472, 602, 677
BshNI GGYRCC 1 cut(s) 294
BsiEI CGRYCG 2 cut(s) 57, 196
BsiSI CCGG 7 cut(s) 23, 57, 109, 169, 192, 210, 342
BslFI GGGAC 2 cut(s) 69, 430
BslI CCNNNNNNNGG 5 cut(s) 133, 311, 437, 556, 625
BsmAI GTCTC 1 cut(s) 496
BsmFI GGGAC 2 cut(s) 69, 430
BsnI GGCC 7 cut(s) 5, 112, 137, 146, 472, 602, 677
Bsp119I TTCGAA 1 cut(s) 779
Bsp120I GGGCCC 1 cut(s) 470
Bsp1286I GDGCHC 1 cut(s) 474
Bsp143I GATC 5 cut(s) 75, 151, 217, 351, 462
Bsp68I TCGCGA 1 cut(s) 380
BspACI CCGC 8 cut(s) 7, 46, 228, 292, 332, 339, 500, 518
BspANI GGCC 7 cut(s) 5, 112, 137, 146, 472, 602, 677
BspFNI CGCG 9 cut(s) 48, 65, 198, 200, 332, 356, 380, 484, 486
BspHI TCATGA 1 cut(s) 709
BspLI GGNNCC 3 cut(s) 190, 296, 472
BspMI ACCTGC 1 cut(s) 758
BspPI GGATC 1 cut(s) 470
BspT104I TTCGAA 1 cut(s) 779
BspT107I GGYRCC 1 cut(s) 294
BspTI CTTAAG 1 cut(s) 662
BsrBI CCGCTC 1 cut(s) 292
BsrDI GCAATG 1 cut(s) 603
BsrI ACTGG 1 cut(s) 739
BssECI CCNNGG 2 cut(s) 132, 231
BssHII GCGCGC 1 cut(s) 482
BssMI GATC 5 cut(s) 75, 151, 217, 351, 462
BssNI GRCGYC 3 cut(s) 213, 506, 681
BssSI CACGAG 1 cut(s) 585
Bst2BI CACGAG 1 cut(s) 585
Bst2UI CCWGG 2 cut(s) 134, 674
Bst4CI ACNGT 1 cut(s) 83
Bst6I CTCTTC 1 cut(s) 555
BstACI GRCGYC 3 cut(s) 213, 506, 681
BstAFI CTTAAG 1 cut(s) 662
BstBI TTCGAA 1 cut(s) 779
BstC8I GCNNGC 5 cut(s) 7, 360, 364, 484, 498
BstEII GGTNACC 1 cut(s) 334
BstENI CCTNNNNNAGG 1 cut(s) 131
BstFNI CGCG 9 cut(s) 48, 65, 198, 200, 332, 356, 380, 484, 486
BstHHI GCGC 4 cut(s) 200, 358, 484, 486
BstKTI GATC 5 cut(s) 78, 154, 220, 354, 465
BstMAI GTCTC 1 cut(s) 496
BstMBI GATC 5 cut(s) 75, 151, 217, 351, 462
BstMCI CGRYCG 2 cut(s) 57, 196
BstMWI GCNNNNNNNGC 4 cut(s) 100, 109, 143, 338
BstNI CCWGG 2 cut(s) 134, 674
BstPI GGTNACC 1 cut(s) 334
BstSCI CCNGG 5 cut(s) 107, 132, 168, 341, 672
BstSLI GKGCMC 1 cut(s) 474
BstUI CGCG 9 cut(s) 48, 65, 198, 200, 332, 356, 380, 484, 486
BsuRI GGCC 7 cut(s) 5, 112, 137, 146, 472, 602, 677
BtgZI GCGATG 2 cut(s) 264, 361
BtrI CACGTC 1 cut(s) 262
BtuMI TCGCGA 1 cut(s) 380
BveI ACCTGC 1 cut(s) 758
Cac8I GCNNGC 5 cut(s) 7, 360, 364, 484, 498
CciI TCATGA 1 cut(s) 709
CfoI GCGC 4 cut(s) 200, 358, 484, 486
Cfr13I GGNCC 9 cut(s) 4, 111, 124, 145, 166, 189, 470, 471, 670
CpoI CGGWCCG 1 cut(s) 166
CseI GACGC 3 cut(s) 202, 514, 689
Csp6I GTAC 1 cut(s) 637
CspI CGGWCCG 1 cut(s) 166
CviAII CATG 5 cut(s) 115, 400, 620, 625, 710
CviQI GTAC 1 cut(s) 637
DpnI GATC 5 cut(s) 77, 153, 219, 353, 464
DpnII GATC 5 cut(s) 75, 151, 217, 351, 462
DraIII CACNNNGTG 1 cut(s) 556
DrdI GACNNNNNNGTC 1 cut(s) 164
DriI GACNNNNNGTC 1 cut(s) 265
DseDI GACNNNNNNGTC 1 cut(s) 164
EaeI YGGCCR 1 cut(s) 675
Eam1104I CTCTTC 1 cut(s) 555
Eam1105I GACNNNNNGTC 1 cut(s) 265
EarI CTCTTC 1 cut(s) 555
EciI GGCGGA 1 cut(s) 217
Eco147I AGGCCT 1 cut(s) 137
Eco24I GRGCYC 1 cut(s) 474
Eco32I GATATC 1 cut(s) 370
Eco47I GGWCC 4 cut(s) 124, 166, 189, 670
Eco57I CTGAAG 1 cut(s) 596
Eco91I GGTNACC 1 cut(s) 334
EcoNI CCTNNNNNAGG 1 cut(s) 131
EcoO109I RGGNCCY 2 cut(s) 145, 470
EcoO65I GGTNACC 1 cut(s) 334
EcoRII CCWGG 2 cut(s) 132, 672
EcoRV GATATC 1 cut(s) 370
EcoT38I GRGCYC 1 cut(s) 474
FaeI CATG 5 cut(s) 118, 403, 623, 628, 713
FaiI YATR 8 cut(s) 35, 116, 401, 467, 621, 626, 711, 764
FaqI GGGAC 2 cut(s) 69, 430
FatI CATG 5 cut(s) 114, 399, 619, 624, 709
FauI CCCGC 1 cut(s) 14
Fnu4HI GCNGC 1 cut(s) 501
FriOI GRGCYC 1 cut(s) 474
Fsp4HI GCNGC 1 cut(s) 501
FspBI CTAG 2 cut(s) 149, 414
GlaI GCGC 4 cut(s) 199, 357, 483, 485
GluI GCNGC 1 cut(s) 501
HaeIII GGCC 7 cut(s) 5, 112, 137, 146, 472, 602, 677
HapII CCGG 7 cut(s) 23, 57, 109, 169, 192, 210, 342
HgaI GACGC 3 cut(s) 202, 514, 689
HhaI GCGC 4 cut(s) 200, 358, 484, 486
Hin1I GRCGYC 3 cut(s) 213, 506, 681
Hin1II CATG 5 cut(s) 118, 403, 623, 628, 713
Hin6I GCGC 4 cut(s) 198, 356, 482, 484
HinP1I GCGC 4 cut(s) 198, 356, 482, 484
HincII GTYRAC 1 cut(s) 513
HindII GTYRAC 1 cut(s) 513
HindIII AAGCTT 1 cut(s) 452
HinfI GANTC 2 cut(s) 60, 161
HpaII CCGG 7 cut(s) 23, 57, 109, 169, 192, 210, 342
HphI GGTGA 3 cut(s) 214, 328, 608
Hpy166II GTNNAC 3 cut(s) 43, 513, 547
Hpy188I TCNGA 3 cut(s) 271, 314, 774
Hpy188III TCNNGA 7 cut(s) 70, 155, 349, 379, 544, 587, 710
Hpy8I GTNNAC 3 cut(s) 43, 513, 547
Hpy99I CGWCG 6 cut(s) 22, 218, 385, 436, 508, 683
HpyAV CCTTC 3 cut(s) 148, 250, 798
HpyCH4III ACNGT 1 cut(s) 83
HpyCH4IV ACGT 3 cut(s) 237, 261, 552
HpyCH4V TGCA 2 cut(s) 265, 754
HpyF10VI GCNNNNNNNGC 4 cut(s) 100, 109, 143, 338
HpySE526I ACGT 3 cut(s) 237, 261, 552
Hsp92I GRCGYC 3 cut(s) 213, 506, 681
Hsp92II CATG 5 cut(s) 118, 403, 623, 628, 713
HspAI GCGC 4 cut(s) 198, 356, 482, 484
Kzo9I GATC 5 cut(s) 75, 151, 217, 351, 462
LmnI GCTCC 3 cut(s) 289, 314, 389
LweI GCATC 1 cut(s) 741
MaeI CTAG 2 cut(s) 149, 414
MaeII ACGT 3 cut(s) 237, 261, 552
MaeIII GTNAC 2 cut(s) 70, 334
MalI GATC 5 cut(s) 77, 153, 219, 353, 464
MbiI CCGCTC 1 cut(s) 292
MboI GATC 5 cut(s) 75, 151, 217, 351, 462
MboII GAAGA 4 cut(s) 572, 589, 760, 783
MhlI GDGCHC 1 cut(s) 474
MluCI AATT 2 cut(s) 318, 395
MmeI TCCRAC 2 cut(s) 294, 752
MnlI CCTC 7 cut(s) 226, 235, 453, 514, 592, 627, 696
MseI TTAA 3 cut(s) 321, 663, 744
MspA1I CMGCKG 1 cut(s) 518
MspCI CTTAAG 1 cut(s) 662
MspI CCGG 7 cut(s) 23, 57, 109, 169, 192, 210, 342
MspR9I CCNGG 5 cut(s) 109, 134, 170, 343, 674
MvaI CCWGG 2 cut(s) 134, 674
MvnI CGCG 9 cut(s) 48, 65, 198, 200, 332, 356, 380, 484, 486
MwoI GCNNNNNNNGC 4 cut(s) 100, 109, 143, 338
NciI CCSGG 3 cut(s) 109, 170, 343
NdeII GATC 5 cut(s) 75, 151, 217, 351, 462
NlaIII CATG 5 cut(s) 118, 403, 623, 628, 713
NlaIV GGNNCC 3 cut(s) 190, 296, 472
NmeAIII GCCGAG 1 cut(s) 256
NmuCI GTSAC 2 cut(s) 70, 334
NruI TCGCGA 1 cut(s) 380
NspV TTCGAA 1 cut(s) 779
PagI TCATGA 1 cut(s) 709
PauI GCGCGC 1 cut(s) 482
PceI AGGCCT 1 cut(s) 137
PcsI WCGNNNNNNNCGW 3 cut(s) 267, 500, 549
PfeI GAWTC 2 cut(s) 60, 161
PflMI CCANNNNNTGG 2 cut(s) 556, 625
PkrI GCNGC 1 cut(s) 502
Psp6I CCWGG 2 cut(s) 132, 672
PspEI GGTNACC 1 cut(s) 334
PspGI CCWGG 2 cut(s) 132, 672
PspN4I GGNNCC 3 cut(s) 190, 296, 472
PspOMI GGGCCC 1 cut(s) 470
PspPI GGNCC 9 cut(s) 4, 111, 124, 145, 166, 189, 470, 471, 670
PteI GCGCGC 1 cut(s) 482
RruI TCGCGA 1 cut(s) 380
RsaI GTAC 1 cut(s) 638
RsaNI GTAC 1 cut(s) 637
Rsr2I CGGWCCG 1 cut(s) 166
RsrII CGGWCCG 1 cut(s) 166
SaqAI TTAA 3 cut(s) 321, 663, 744
SatI GCNGC 1 cut(s) 501
Sau3AI GATC 5 cut(s) 75, 151, 217, 351, 462
Sau96I GGNCC 9 cut(s) 4, 111, 124, 145, 166, 189, 470, 471, 670
ScrFI CCNGG 5 cut(s) 109, 134, 170, 343, 674
SduI GDGCHC 1 cut(s) 474
SfaNI GCATC 1 cut(s) 741
SfiI GGCCNNNNNGGCC 1 cut(s) 143
SfuI TTCGAA 1 cut(s) 779
SinI GGWCC 4 cut(s) 124, 166, 189, 670
SmlI CTYRAG 1 cut(s) 662
SmoI CTYRAG 1 cut(s) 662
Sse9I AATT 2 cut(s) 318, 395
SseBI AGGCCT 1 cut(s) 137
SsiI CCGC 8 cut(s) 7, 46, 228, 292, 332, 339, 500, 518
SspMI CTAG 2 cut(s) 149, 414
StuI AGGCCT 1 cut(s) 137
StyD4I CCNGG 5 cut(s) 107, 132, 168, 341, 672
TaaI ACNGT 1 cut(s) 83
TaiI ACGT 3 cut(s) 240, 264, 555
TaqI TCGA 4 cut(s) 216, 348, 458, 779
TaqII GACCGA 1 cut(s) 154
TasI AATT 2 cut(s) 318, 395
TauI GCSGC 1 cut(s) 503
TfiI GAWTC 2 cut(s) 60, 161
Tru1I TTAA 3 cut(s) 321, 663, 744
Tru9I TTAA 3 cut(s) 321, 663, 744
TseFI GTSAC 2 cut(s) 70, 334
Tsp45I GTSAC 2 cut(s) 70, 334
TspDTI ATGAA 2 cut(s) 388, 751
TspGWI ACGGA 2 cut(s) 137, 420
Van91I CCANNNNNTGG 2 cut(s) 556, 625
Vha464I CTTAAG 1 cut(s) 662
VpaK11BI GGWCC 4 cut(s) 124, 166, 189, 670
XagI CCTNNNNNAGG 1 cut(s) 131
XapI RAATTY 1 cut(s) 395
XspI CTAG 2 cut(s) 149, 414
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.