pycom16g13410

Belongs to the importin alpha family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0008963
Physical Location & Seq
Forward (+)
9579796 .. 9582995
3200 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1455 bp
ATGTCGCTGAGGCCGAGCGCGAGGACCGAGGTCCGCCGGAACCGCTACAAGGTGGCGGTGGACGCCGAGGAGGGACGTCGCAGGAGAGAGGACAACATGGTGGAGATCCGGAAGAGCAAGCGCGAAGAGAGCTTGCTGAAGAAGCGTCGCGAAGGCCTCCAAGCCCAGCAGTTCGCGCCATCTCTCCAGCCCTCAAATCTTGAGAAGAAGTTAGAGAGTCTTCCTGCCATGGTTGCTGGGGTTTGGTCTGACGACAACAATTTGCAGCTGGAGTCCACTACGCAGTTCAGAAAACTTCTTTCGATTGAACGAAGCCCTCCGATTGAGGAAGTTATCCAATCTGGAGTGGTTCCTCGCTTTGTCACGTTCCTCATGAGAGAGGATTTTCCACAACTTCAGTTTGAGGCTGCTTGGGCTCTTACAAACATAGCGTCAGGAACCTCAGAGAACACCAAGGTGGTGATTGAGCAGGGGGCAGTCCCAATCTTTGTAAAGCTTCTTGCTTCTCCGAGTGATGATGTTAGGGAGCAGGCTGTGTGGGCATTGGGAAATGTTGCTGGTGATTCCCCTAGATGCCGTGATCTTGTCCTTGGCAGTGGAGCCTTGATGCCGTTGCTGGCTCAGTTGAATGAGCATGCAAAGCTTTCAATGCTGAGAAATGCAACATGGACTCTGTCTAACTTTTGCAGGGGCAAGCCACAGCCTCCATTTGACCAGGTGAAGCCAGCACTTCCAGCTCTTGAACGTTTAGTTCATTCCAATGATGAAGAAGTCTTGACAGATGCCTGCTGGGCACTTTCATATCTCTCTGATGGTACGAATGACAAAATCCAAGCTGTGATTGAGGCAGGTGTCTGCGTCCGACTTGTGCAGCTCCTCTTACATCCATCTCCTTCTGTTCTCATTCCGGCACTTCGCACTGTTGGTAATATTGTGACTGGAGATGATCTTCAGACTCAGTGTATTATTAACAATGGTTCACTCCCATGCCTTCTGAGCCTGTTGACTCATAATCATAAAAAGAGCATCAAGAAAGAAGCTTGCTGGACAATCTCAAACATTACAGCCGGAAATATCGATCAAATACAGTCTGTAATTGAAGCTGGTTTGATCGGTCCTCTGGTCAACTTGCTTCAACATGCTGAGTTTGACATAAAGAAAGAAGCTGCATGGGCAATTTCAAATGCTACTTCTGGTGGAACTCCTGATCAAATCAAGTACCTGGTTGGCGAAGGCTGTATAAAACCATTGTGTGATCTTCTTGTATGCCCTGATCCAAGGATCGTTACTGTCTGTTTAGAAGGGTTGGAGAACATTCTGAAGGTTGGGGAAGCTGAAAAAGCTGCGGGTAACAGTGGAGAAGTTAACTTGTATGCCCAGCTGATTGATGATGCTGAGGGTCTAGAAAAGATTGAAAATCTACAGAGTCACGACAACACTGATCAAGATCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Arm Arm_3 IBB

Protein Analysis

485

Amino Acids

53.21

Weight (kDa)

5.28

Isoelectric Point (pI)

53.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IBB PF01749 12 - 94 1.5e-23 Importin beta binding domain
Arm PF00514 105 - 145 8.3e-11 Armadillo/beta-catenin-like repeat
ARM_ARMC5 PF24768 108 - 230 7.2e-06 ARMC5
HEAT_EZ PF13513 130 - 185 2.7e-10 HEAT-like repeat
Arm PF00514 147 - 186 1e-12 Armadillo/beta-catenin-like repeat
HEAT_2 PF13646 161 - 267 1.6e-06 HEAT repeats
Arm PF00514 189 - 230 5.6e-07 Armadillo/beta-catenin-like repeat
Arm PF00514 243 - 271 3.5e-07 Armadillo/beta-catenin-like repeat
Arm PF00514 274 - 312 2.9e-08 Armadillo/beta-catenin-like repeat
ARM_ARMC5 PF24768 304 - 470 4.7e-09 ARMC5
Arm PF00514 316 - 356 1.3e-09 Armadillo/beta-catenin-like repeat
Arm PF00514 360 - 397 1.9e-11 Armadillo/beta-catenin-like repeat
Arm PF00514 401 - 440 1.2e-07 Armadillo/beta-catenin-like repeat
Arm_3 PF16186 456 - 481 5.7e-08 Atypical Arm repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0008963)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G32880 AT3G06720 AT3G06720 AT4G16143 AT4G16143
fragaria_vesca FvH4_4g19270 FvH4_4g19270
malus_domestica MD13G1158900.v1.1 MD16G1159100.v1.1
prunus_persica Prupe.1G125900_v2.0.a1
pyrus_communis pycom16g13410
rosa_chinensis RchiOBHm_Chr4g0424261
rosa_laevigata RLG00000007492
rosa_multiflora Rmu_sc0006403.1_g000001
rosa_roxburghii Rroxscaffold_5G00366200
rosa_rugosa Rorug04G0194700 Rorug04G0194800
rosa_samantha Rh4AG252100 Rh4BG257500 Rh4CG268200 Rh4DG251900
rosa_wichuraiana Rw4G021740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 839
AatII GACGTC 1 cut(s) 79
Acc36I ACCTGC 1 cut(s) 839
AccII CGCG 4 cut(s) 20, 123, 150, 176
AccIII TCCGGA 1 cut(s) 108
AciI CCGC 4 cut(s) 34, 43, 56, 1346
AclI AACGTT 1 cut(s) 745
AclWI GGATC 4 cut(s) 100, 1268, 1289, 1442
AcuI CTGAAG 4 cut(s) 158, 380, 935, 1340
AcyI GRCGYC 2 cut(s) 63, 76
AfaI GTAC 2 cut(s) 817, 1220
AfiI CCNNNNNNNGG 1 cut(s) 49
AgsI TTSAA 8 cut(s) 308, 628, 648, 743, 1100, 1136, 1182, 1415
AjnI CCWGG 2 cut(s) 714, 1221
AleI CACNNNNGTG 1 cut(s) 455
AlwI GGATC 4 cut(s) 100, 1268, 1289, 1442
Aor13HI TCCGGA 1 cut(s) 108
AoxI GGCC 2 cut(s) 11, 154
ApeKI GCWGC 5 cut(s) 265, 407, 871, 1166, 1343
ArsI GACNNNNNNTTYG 2 cut(s) 416, 448
AspLEI GCGC 3 cut(s) 20, 123, 178
AspS9I GGNCC 3 cut(s) 24, 31, 1115
AsuHPI GGTGA 3 cut(s) 472, 572, 730
AvaII GGWCC 3 cut(s) 24, 31, 1115
BaeGI GKGCMC 1 cut(s) 796
BanII GRGCYC 1 cut(s) 418
BbsI GAAGAC 1 cut(s) 212
BbvCI CCTCAGC 2 cut(s) 8, 1395
BbvI GCAGC 5 cut(s) 277, 394, 883, 1153, 1330
BccI CCATC 3 cut(s) 187, 806, 895
BceAI ACGGC 2 cut(s) 561, 595
BciT130I CCWGG 2 cut(s) 716, 1223
BclI TGATCA 2 cut(s) 1207, 1441
BfaI CTAG 2 cut(s) 570, 1403
BfmI CTRYAG 1 cut(s) 1421
BfuAI ACCTGC 1 cut(s) 839
BglI GCCNNNNNGGC 1 cut(s) 791
BisI GCNGC 5 cut(s) 266, 408, 872, 1167, 1344
BlsI GCNGC 5 cut(s) 267, 409, 873, 1168, 1345
Bme1390I CCNGG 2 cut(s) 716, 1223
Bme18I GGWCC 3 cut(s) 24, 31, 1115
BmgT120I GGNCC 3 cut(s) 24, 31, 1115
BmiI GGNNCC 4 cut(s) 41, 351, 439, 601
BmrFI CCNGG 2 cut(s) 716, 1223
BmsI GCATC 5 cut(s) 563, 597, 772, 1035, 1381
BoxI GACNNNNGTC 1 cut(s) 29
BpiI GAAGAC 1 cut(s) 212
BpmI CTGGAG 4 cut(s) 170, 290, 363, 960
Bpu10I CCTNAGC 2 cut(s) 8, 1395
BpuEI CTTGAG 1 cut(s) 221
Bsa29I ATCGAT 1 cut(s) 1077
BsaBI GATNNNNATC 1 cut(s) 1446
BsaHI GRCGYC 2 cut(s) 63, 76
BsaJI CCNNGG 6 cut(s) 27, 66, 228, 453, 589, 1277
BsaWI WCCGGW 1 cut(s) 108
Bsc4I CCNNNNNNNGG 1 cut(s) 49
Bse1I ACTGG 1 cut(s) 943
Bse8I GATNNNNATC 1 cut(s) 1446
BseAI TCCGGA 1 cut(s) 108
BseBI CCWGG 2 cut(s) 716, 1223
BseCI ATCGAT 1 cut(s) 1077
BseDI CCNNGG 6 cut(s) 27, 66, 228, 453, 589, 1277
BseGI GGATG 1 cut(s) 883
BseJI GATNNNNATC 1 cut(s) 1446
BseLI CCNNNNNNNGG 1 cut(s) 49
BseMII CTCAG 7 cut(s) 456, 635, 644, 971, 986, 1134, 1386
BseNI ACTGG 1 cut(s) 943
BseRI GAGGAG 2 cut(s) 83, 866
BseSI GKGCMC 1 cut(s) 796
BseXI GCAGC 5 cut(s) 277, 394, 883, 1153, 1330
BseYI CCCAGC 4 cut(s) 165, 236, 789, 1377
BsgI GTGCAG 1 cut(s) 890
Bsh1236I CGCG 4 cut(s) 20, 123, 150, 176
BshFI GGCC 2 cut(s) 13, 156
BshVI ATCGAT 1 cut(s) 1077
BsiSI CCGG 4 cut(s) 37, 109, 908, 1068
BslFI GGGAC 2 cut(s) 87, 464
BslI CCNNNNNNNGG 1 cut(s) 49
BsmFI GGGAC 2 cut(s) 87, 464
BsnI GGCC 2 cut(s) 13, 156
Bsp1286I GDGCHC 2 cut(s) 418, 796
Bsp13I TCCGGA 1 cut(s) 108
Bsp19I CCATGG 1 cut(s) 228
Bsp68I TCGCGA 1 cut(s) 150
BspACI CCGC 4 cut(s) 34, 43, 56, 1346
BspANI GGCC 2 cut(s) 13, 156
BspCNI CTCAG 7 cut(s) 455, 634, 645, 970, 987, 1135, 1387
BspDI ATCGAT 1 cut(s) 1077
BspEI TCCGGA 1 cut(s) 108
BspFNI CGCG 4 cut(s) 20, 123, 150, 176
BspHI TCATGA 1 cut(s) 372
BspLI GGNNCC 4 cut(s) 41, 351, 439, 601
BspMI ACCTGC 1 cut(s) 839
BspPI GGATC 4 cut(s) 100, 1268, 1289, 1442
BspQI GCTCTTC 1 cut(s) 107
BsrI ACTGG 1 cut(s) 943
BssECI CCNNGG 6 cut(s) 27, 66, 228, 453, 589, 1277
BssNI GRCGYC 2 cut(s) 63, 76
BssT1I CCWWGG 4 cut(s) 228, 453, 589, 1277
Bst2UI CCWGG 2 cut(s) 716, 1223
Bst4CI ACNGT 4 cut(s) 922, 1089, 1291, 1355
Bst6I CTCTTC 2 cut(s) 107, 120
BstACI GRCGYC 2 cut(s) 63, 76
BstC8I GCNNGC 9 cut(s) 119, 134, 531, 618, 636, 695, 726, 787, 1042
BstDEI CTNAG 8 cut(s) 8, 442, 621, 653, 957, 995, 1143, 1395
BstDSI CCRYGG 1 cut(s) 228
BstF5I GGATG 1 cut(s) 883
BstFNI CGCG 4 cut(s) 20, 123, 150, 176
BstHHI GCGC 3 cut(s) 20, 123, 178
BstNI CCWGG 2 cut(s) 716, 1223
BstNSI RCATGY 2 cut(s) 638, 1142
BstPAI GACNNNNGTC 1 cut(s) 29
BstSCI CCNGG 2 cut(s) 714, 1221
BstSFI CTRYAG 1 cut(s) 1421
BstSLI GKGCMC 1 cut(s) 796
BstUI CGCG 4 cut(s) 20, 123, 150, 176
BstV1I GCAGC 5 cut(s) 277, 394, 883, 1153, 1330
BstV2I GAAGAC 1 cut(s) 212
BstX2I RGATCY 2 cut(s) 105, 1447
BstYI RGATCY 2 cut(s) 105, 1447
Bsu15I ATCGAT 1 cut(s) 1077
BsuRI GGCC 2 cut(s) 13, 156
BsuTUI ATCGAT 1 cut(s) 1077
BtgI CCRYGG 1 cut(s) 228
BtsCI GGATG 1 cut(s) 883
BtsI GCAGTG 1 cut(s) 601
BtsIMutI CAGTG 5 cut(s) 601, 918, 965, 1360, 1437
BtuMI TCGCGA 1 cut(s) 150
BveI ACCTGC 1 cut(s) 839
Cac8I GCNNGC 9 cut(s) 119, 134, 531, 618, 636, 695, 726, 787, 1042
CciI TCATGA 1 cut(s) 372
CfoI GCGC 3 cut(s) 20, 123, 178
Cfr13I GGNCC 3 cut(s) 24, 31, 1115
ClaI ATCGAT 1 cut(s) 1077
CseI GACGC 4 cut(s) 71, 134, 420, 847
CsiI ACCWGGT 2 cut(s) 714, 1221
Csp6I GTAC 2 cut(s) 816, 1219
CviAII CATG 9 cut(s) 97, 229, 373, 635, 666, 987, 1139, 1170, 1452
CviQI GTAC 2 cut(s) 816, 1219
DdeI CTNAG 8 cut(s) 8, 442, 621, 653, 957, 995, 1143, 1395
Eam1104I CTCTTC 2 cut(s) 107, 120
EarI CTCTTC 2 cut(s) 107, 120
EciI GGCGGA 1 cut(s) 23
Eco130I CCWWGG 4 cut(s) 228, 453, 589, 1277
Eco147I AGGCCT 1 cut(s) 156
Eco24I GRGCYC 1 cut(s) 418
Eco47I GGWCC 3 cut(s) 24, 31, 1115
Eco57I CTGAAG 4 cut(s) 158, 380, 935, 1340
EcoRII CCWGG 2 cut(s) 714, 1221
EcoT14I CCWWGG 4 cut(s) 228, 453, 589, 1277
EcoT38I GRGCYC 1 cut(s) 418
ErhI CCWWGG 4 cut(s) 228, 453, 589, 1277
FaeI CATG 9 cut(s) 100, 232, 376, 638, 669, 990, 1142, 1173, 1455
FaqI GGGAC 2 cut(s) 87, 464
FatI CATG 9 cut(s) 96, 228, 372, 634, 665, 986, 1138, 1169, 1451
FauI CCCGC 1 cut(s) 1339
FbaI TGATCA 2 cut(s) 1207, 1441
Fnu4HI GCNGC 5 cut(s) 266, 408, 872, 1167, 1344
FokI GGATG 1 cut(s) 870
FriOI GRGCYC 1 cut(s) 418
Fsp4HI GCNGC 5 cut(s) 266, 408, 872, 1167, 1344
FspBI CTAG 2 cut(s) 570, 1403
GlaI GCGC 3 cut(s) 19, 122, 177
GluI GCNGC 5 cut(s) 266, 408, 872, 1167, 1344
GsaI CCCAGC 4 cut(s) 169, 240, 793, 1381
GsuI CTGGAG 4 cut(s) 170, 290, 363, 960
HaeIII GGCC 2 cut(s) 13, 156
HapII CCGG 4 cut(s) 37, 109, 908, 1068
HgaI GACGC 4 cut(s) 71, 134, 420, 847
HhaI GCGC 3 cut(s) 20, 123, 178
Hin1I GRCGYC 2 cut(s) 63, 76
Hin1II CATG 9 cut(s) 100, 232, 376, 638, 669, 990, 1142, 1173, 1455
Hin6I GCGC 3 cut(s) 18, 121, 176
HinP1I GCGC 3 cut(s) 18, 121, 176
HincII GTYRAC 3 cut(s) 1005, 1126, 1366
HindII GTYRAC 3 cut(s) 1005, 1126, 1366
HindIII AAGCTT 3 cut(s) 494, 641, 1038
HinfI GANTC 7 cut(s) 217, 272, 563, 670, 955, 1006, 1426
HpaI GTTAAC 1 cut(s) 1366
HpaII CCGG 4 cut(s) 37, 109, 908, 1068
HphI GGTGA 3 cut(s) 472, 572, 730
Hpy166II GTNNAC 6 cut(s) 61, 276, 980, 1005, 1126, 1366
Hpy8I GTNNAC 6 cut(s) 61, 276, 980, 1005, 1126, 1366
Hpy99I CGWCG 2 cut(s) 81, 150
HpyAV CCTTC 6 cut(s) 146, 903, 1001, 1226, 1295, 1315
HpyCH4III ACNGT 4 cut(s) 922, 1089, 1291, 1355
HpyCH4IV ACGT 3 cut(s) 76, 365, 745
HpyCH4V TGCA 6 cut(s) 265, 638, 662, 687, 871, 1169
HpyF3I CTNAG 8 cut(s) 8, 442, 621, 653, 957, 995, 1143, 1395
HpySE526I ACGT 3 cut(s) 76, 365, 745
Hsp92I GRCGYC 2 cut(s) 63, 76
Hsp92II CATG 9 cut(s) 100, 232, 376, 638, 669, 990, 1142, 1173, 1455
HspAI GCGC 3 cut(s) 18, 121, 176
Kpn2I TCCGGA 1 cut(s) 108
Ksp22I TGATCA 2 cut(s) 1207, 1441
KspAI GTTAAC 1 cut(s) 1366
LguI GCTCTTC 1 cut(s) 107
LmnI GCTCC 3 cut(s) 526, 599, 879
Lsp1109I GCAGC 5 cut(s) 277, 394, 883, 1153, 1330
LweI GCATC 5 cut(s) 563, 597, 772, 1035, 1381
MabI ACCWGGT 2 cut(s) 714, 1221
MaeI CTAG 2 cut(s) 570, 1403
MaeII ACGT 3 cut(s) 76, 365, 745
MaeIII GTNAC 5 cut(s) 361, 934, 1285, 1349, 1427
MboII GAAGA 8 cut(s) 124, 137, 151, 212, 217, 779, 941, 1250
MflI RGATCY 2 cut(s) 105, 1447
MhlI GDGCHC 2 cut(s) 418, 796
MluCI AATT 3 cut(s) 259, 1095, 1176
MlyI GAGTC 6 cut(s) 226, 281, 664, 949, 1000, 1435
MmeI TCCRAC 2 cut(s) 886, 1287
MroI TCCGGA 1 cut(s) 108
MseI TTAA 2 cut(s) 969, 1365
MslI CAYNNNNRTG 3 cut(s) 455, 759, 985
MspA1I CMGCKG 2 cut(s) 268, 1381
MspI CCGG 4 cut(s) 37, 109, 908, 1068
MspR9I CCNGG 2 cut(s) 716, 1223
MvaI CCWGG 2 cut(s) 716, 1223
MvnI CGCG 4 cut(s) 20, 123, 150, 176
NcoI CCATGG 1 cut(s) 228
NlaIII CATG 9 cut(s) 100, 232, 376, 638, 669, 990, 1142, 1173, 1455
NlaIV GGNNCC 4 cut(s) 41, 351, 439, 601
NmeAIII GCCGAG 2 cut(s) 39, 91
NmuCI GTSAC 3 cut(s) 361, 934, 1427
NruI TCGCGA 1 cut(s) 150
NspI RCATGY 2 cut(s) 638, 1142
OliI CACNNNNGTG 1 cut(s) 455
PaeI GCATGC 1 cut(s) 638
PagI TCATGA 1 cut(s) 372
PaqCI CACCTGC 1 cut(s) 839
PceI AGGCCT 1 cut(s) 156
PciSI GCTCTTC 1 cut(s) 107
PcsI WCGNNNNNNNCGW 1 cut(s) 11
PfeI GAWTC 1 cut(s) 563
PflFI GACNNNGTC 1 cut(s) 673
PkrI GCNGC 5 cut(s) 267, 409, 873, 1168, 1345
PleI GAGTC 6 cut(s) 225, 280, 664, 949, 1000, 1434
PpsI GAGTC 6 cut(s) 225, 280, 664, 949, 1000, 1434
PshAI GACNNNNGTC 1 cut(s) 29
Psp1406I AACGTT 1 cut(s) 745
Psp6I CCWGG 2 cut(s) 714, 1221
PspFI CCCAGC 4 cut(s) 165, 236, 789, 1377
PspGI CCWGG 2 cut(s) 714, 1221
PspN4I GGNNCC 4 cut(s) 41, 351, 439, 601
PspPI GGNCC 3 cut(s) 24, 31, 1115
PsuI RGATCY 2 cut(s) 105, 1447
PsyI GACNNNGTC 1 cut(s) 673
PvuII CAGCTG 2 cut(s) 268, 1381
RruI TCGCGA 1 cut(s) 150
RsaI GTAC 2 cut(s) 817, 1220
RsaNI GTAC 2 cut(s) 816, 1219
RseI CAYNNNNRTG 3 cut(s) 455, 759, 985
SapI GCTCTTC 1 cut(s) 107
SaqAI TTAA 2 cut(s) 969, 1365
SatI GCNGC 5 cut(s) 266, 408, 872, 1167, 1344
Sau96I GGNCC 3 cut(s) 24, 31, 1115
SchI GAGTC 6 cut(s) 226, 281, 664, 949, 1000, 1435
ScrFI CCNGG 2 cut(s) 716, 1223
SduI GDGCHC 2 cut(s) 418, 796
SexAI ACCWGGT 2 cut(s) 714, 1221
SfaNI GCATC 5 cut(s) 563, 597, 772, 1035, 1381
SfcI CTRYAG 1 cut(s) 1421
SinI GGWCC 3 cut(s) 24, 31, 1115
SmiMI CAYNNNNRTG 3 cut(s) 455, 759, 985
SmlI CTYRAG 1 cut(s) 200
SmoI CTYRAG 1 cut(s) 200
SphI GCATGC 1 cut(s) 638
Sse9I AATT 3 cut(s) 259, 1095, 1176
SseBI AGGCCT 1 cut(s) 156
SsiI CCGC 4 cut(s) 34, 43, 56, 1346
SspI AATATT 1 cut(s) 931
SspMI CTAG 2 cut(s) 570, 1403
StuI AGGCCT 1 cut(s) 156
StyD4I CCNGG 2 cut(s) 714, 1221
StyI CCWWGG 4 cut(s) 228, 453, 589, 1277
TaaI ACNGT 4 cut(s) 922, 1089, 1291, 1355
TaiI ACGT 3 cut(s) 79, 368, 748
TaqI TCGA 2 cut(s) 302, 1077
TaqII GACCGA 2 cut(s) 41, 1103
TasI AATT 3 cut(s) 259, 1095, 1176
TfiI GAWTC 1 cut(s) 563
Tru1I TTAA 2 cut(s) 969, 1365
Tru9I TTAA 2 cut(s) 969, 1365
TscAI CASTG 5 cut(s) 601, 925, 965, 1360, 1444
TseFI GTSAC 3 cut(s) 361, 934, 1427
TseI GCWGC 5 cut(s) 265, 407, 871, 1166, 1343
Tsp45I GTSAC 3 cut(s) 361, 934, 1427
TspDTI ATGAA 3 cut(s) 743, 780, 789
TspRI CASTG 5 cut(s) 601, 925, 965, 1360, 1444
Tth111I GACNNNGTC 1 cut(s) 673
VpaK11BI GGWCC 3 cut(s) 24, 31, 1115
XbaI TCTAGA 1 cut(s) 1402
XceI RCATGY 2 cut(s) 638, 1142
XspI CTAG 2 cut(s) 570, 1403
ZraI GACGTC 1 cut(s) 77
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.