pycom16g16220

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0007712
Physical Location & Seq
Forward (+)
12168808 .. 12169619
812 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g16220.1

Sequence Viewer

Length: 687 bp
ATGAGTCCAGTAACTATTTTTTTTCTCTTTTCTTTTTTATCTTTTTTTCCTTTTTTTTTCTTTTTTCTTTTTTCTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTCTTCGGTGCGAACAAACACAGCAGCAAGATCCTTTCAATTTTCTTATTTTGTTGCTTCTGGCGATGATAGTCTGTGGCAGAGTACAAGGCGAGGACGAACGCGATCCGACTTTGGTGTTCTCGGGTCGAGCAGCAACGGTGGTGTGGTGCAGTGGATGCAGGCGACAAGCGGGTCGGGTGATGGTATGAATACAGATCAATGGGTCAGGTCCTGGGTTCCTAATCGCAGCGGTTACACAGGTGCTGGTTCTCAATCGGGATTTGCAGCGGTAATTTCTGGGTTTTTGACCTCGGTTTGTGGCCACGTGATGCAACAGCGGGTAGTGGTGGGTGGCTGCAACGATCGGCTCGGTGAACTCGGATCAGCAACGACGGCATCGCTCGCTGGTCTGGGTGGATGCGGGAATGAACTCGGGTCACCAACGACGGCGCCGCTTGCTGATCTGACGGTGCGCTGCACAATTGGCTGCGCAACTGGTGGGTCTGGGTTCAGCGCACAGTACGATTTCTATATAATTTTTTTTTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

229

Amino Acids

25.36

Weight (kDa)

7.62

Isoelectric Point (pI)

39.5

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 328
Acc16I TGCGCA 1 cut(s) 628
AccB1I GGYRCC 1 cut(s) 586
AccII CGCG 1 cut(s) 259
AciI CCGC 6 cut(s) 327, 387, 425, 475, 558, 590
AclWI GGATC 3 cut(s) 180, 255, 526
AcoI YGGCCR 1 cut(s) 457
AcvI CACGTG 1 cut(s) 463
AcyI GRCGYC 1 cut(s) 587
AfaI GTAC 2 cut(s) 241, 659
AgsI TTSAA 1 cut(s) 194
AjnI CCWGG 1 cut(s) 368
AlwI GGATC 3 cut(s) 180, 255, 526
AlwNI CAGNNNCTG 2 cut(s) 369, 401
Ama87I CYCGRG 2 cut(s) 278, 569
AoxI GGCC 1 cut(s) 457
ApeKI GCWGC 7 cut(s) 179, 288, 384, 422, 492, 612, 624
AspLEI GCGC 4 cut(s) 589, 612, 629, 653
AspS9I GGNCC 1 cut(s) 366
AsuHPI GGTGA 3 cut(s) 347, 521, 567
AvaI CYCGRG 2 cut(s) 278, 569
AvaII GGWCC 1 cut(s) 366
BalI TGGCCA 1 cut(s) 459
BanI GGYRCC 1 cut(s) 586
BbrPI CACGTG 1 cut(s) 463
BbvI GCAGC 7 cut(s) 191, 300, 396, 434, 479, 599, 611
BccI CCATC 1 cut(s) 332
BceAI ACGGC 2 cut(s) 546, 600
BciT130I CCWGG 1 cut(s) 370
BfoI RGCGCY 1 cut(s) 590
BisI GCNGC 8 cut(s) 180, 289, 385, 423, 493, 590, 613, 625
BlsI GCNGC 8 cut(s) 181, 290, 386, 424, 494, 591, 614, 626
Bme1390I CCNGG 1 cut(s) 370
Bme18I GGWCC 1 cut(s) 366
BmeT110I CYCGRG 2 cut(s) 278, 569
BmgT120I GGNCC 1 cut(s) 366
BmiI GGNNCC 2 cut(s) 375, 588
BmrFI CCNGG 1 cut(s) 370
BmsI GCATC 4 cut(s) 303, 456, 542, 545
BsaAI YACGTR 1 cut(s) 463
BsaHI GRCGYC 1 cut(s) 587
BsaJI CCNNGG 2 cut(s) 369, 447
Bse1I ACTGG 2 cut(s) 8, 637
BseBI CCWGG 1 cut(s) 370
BseDI CCNNGG 2 cut(s) 369, 447
BseGI GGATG 2 cut(s) 318, 560
BseNI ACTGG 2 cut(s) 8, 637
BseXI GCAGC 7 cut(s) 191, 300, 396, 434, 479, 599, 611
BsgI GTGCAG 2 cut(s) 326, 598
Bsh1236I CGCG 1 cut(s) 259
Bsh1285I CGRYCG 1 cut(s) 502
BshFI GGCC 1 cut(s) 459
BshNI GGYRCC 1 cut(s) 586
BsiEI CGRYCG 1 cut(s) 502
BsiHKCI CYCGRG 2 cut(s) 278, 569
BsnI GGCC 1 cut(s) 459
BsoBI CYCGRG 2 cut(s) 278, 569
Bsp143I GATC 6 cut(s) 185, 260, 352, 499, 518, 598
BspACI CCGC 6 cut(s) 327, 387, 425, 475, 558, 590
BspANI GGCC 1 cut(s) 459
BspFNI CGCG 1 cut(s) 259
BspLI GGNNCC 2 cut(s) 375, 588
BspPI GGATC 3 cut(s) 180, 255, 526
BspT107I GGYRCC 1 cut(s) 586
BsrI ACTGG 2 cut(s) 8, 637
BssECI CCNNGG 2 cut(s) 369, 447
BssMI GATC 6 cut(s) 185, 260, 352, 499, 518, 598
BssNI GRCGYC 1 cut(s) 587
Bst2UI CCWGG 1 cut(s) 370
Bst4CI ACNGT 3 cut(s) 296, 607, 657
Bst6I CTCTTC 1 cut(s) 163
BstACI GRCGYC 1 cut(s) 587
BstAPI GCANNNNNTGC 1 cut(s) 313
BstBAI YACGTR 1 cut(s) 463
BstC8I GCNNGC 3 cut(s) 318, 540, 594
BstEII GGTNACC 1 cut(s) 573
BstF5I GGATG 2 cut(s) 318, 560
BstFNI CGCG 1 cut(s) 259
BstH2I RGCGCY 1 cut(s) 590
BstHHI GCGC 4 cut(s) 589, 612, 629, 653
BstKTI GATC 6 cut(s) 188, 263, 355, 502, 521, 601
BstMBI GATC 6 cut(s) 185, 260, 352, 499, 518, 598
BstMCI CGRYCG 1 cut(s) 502
BstMWI GCNNNNNNNGC 5 cut(s) 313, 530, 539, 593, 621
BstNI CCWGG 1 cut(s) 370
BstPI GGTNACC 1 cut(s) 573
BstSCI CCNGG 1 cut(s) 368
BstUI CGCG 1 cut(s) 259
BstV1I GCAGC 7 cut(s) 191, 300, 396, 434, 479, 599, 611
BstX2I RGATCY 1 cut(s) 185
BstYI RGATCY 1 cut(s) 185
BsuRI GGCC 1 cut(s) 459
BtgZI GCGATG 2 cut(s) 234, 519
BtsCI GGATG 2 cut(s) 318, 560
BtsI GCAGTG 1 cut(s) 314
BtsIMutI CAGTG 1 cut(s) 314
Cac8I GCNNGC 3 cut(s) 318, 540, 594
CaiI CAGNNNCTG 2 cut(s) 369, 401
CfoI GCGC 4 cut(s) 589, 612, 629, 653
Cfr13I GGNCC 1 cut(s) 366
Csp6I GTAC 2 cut(s) 240, 658
CviJI RGCY 4 cut(s) 459, 492, 505, 624
CviKI_1 RGCY 4 cut(s) 459, 492, 505, 624
CviQI GTAC 2 cut(s) 240, 658
DinI GGCGCC 1 cut(s) 588
DpnI GATC 6 cut(s) 187, 262, 354, 501, 520, 600
DpnII GATC 6 cut(s) 185, 260, 352, 499, 518, 598
DrdI GACNNNNNNGTC 1 cut(s) 328
DseDI GACNNNNNNGTC 1 cut(s) 328
EaeI YGGCCR 1 cut(s) 457
Eam1104I CTCTTC 1 cut(s) 163
EarI CTCTTC 1 cut(s) 163
Eco47I GGWCC 1 cut(s) 366
Eco72I CACGTG 1 cut(s) 463
Eco88I CYCGRG 2 cut(s) 278, 569
Eco91I GGTNACC 1 cut(s) 573
EcoO109I RGGNCCY 1 cut(s) 366
EcoO65I GGTNACC 1 cut(s) 573
EcoRII CCWGG 1 cut(s) 368
EgeI GGCGCC 1 cut(s) 588
EheI GGCGCC 1 cut(s) 588
FaiI YATR 3 cut(s) 344, 669, 671
FauI CCCGC 3 cut(s) 320, 468, 551
Fnu4HI GCNGC 8 cut(s) 180, 289, 385, 423, 493, 590, 613, 625
FokI GGATG 2 cut(s) 325, 567
Fsp4HI GCNGC 8 cut(s) 180, 289, 385, 423, 493, 590, 613, 625
FspI TGCGCA 1 cut(s) 628
GlaI GCGC 4 cut(s) 588, 611, 628, 652
GluI GCNGC 8 cut(s) 180, 289, 385, 423, 493, 590, 613, 625
HaeII RGCGCY 1 cut(s) 590
HaeIII GGCC 1 cut(s) 459
HhaI GCGC 4 cut(s) 589, 612, 629, 653
Hin1I GRCGYC 1 cut(s) 587
Hin6I GCGC 4 cut(s) 587, 610, 627, 651
HinP1I GCGC 4 cut(s) 587, 610, 627, 651
HinfI GANTC 1 cut(s) 4
HphI GGTGA 3 cut(s) 347, 521, 567
Hpy166II GTNNAC 1 cut(s) 512
Hpy188I TCNGA 3 cut(s) 265, 518, 603
Hpy188III TCNNGA 1 cut(s) 414
Hpy8I GTNNAC 1 cut(s) 512
Hpy99I CGWCG 2 cut(s) 532, 586
HpyCH4III ACNGT 3 cut(s) 296, 607, 657
HpyCH4IV ACGT 1 cut(s) 462
HpyCH4V TGCA 6 cut(s) 307, 316, 422, 469, 495, 615
HpyF10VI GCNNNNNNNGC 5 cut(s) 313, 530, 539, 593, 621
HpySE526I ACGT 1 cut(s) 462
Hsp92I GRCGYC 1 cut(s) 587
HspAI GCGC 4 cut(s) 587, 610, 627, 651
KasI GGCGCC 1 cut(s) 586
Kzo9I GATC 6 cut(s) 185, 260, 352, 499, 518, 598
Lsp1109I GCAGC 7 cut(s) 191, 300, 396, 434, 479, 599, 611
LweI GCATC 4 cut(s) 303, 456, 542, 545
MaeII ACGT 1 cut(s) 462
MaeIII GTNAC 3 cut(s) 10, 389, 573
MalI GATC 6 cut(s) 187, 262, 354, 501, 520, 600
MboI GATC 6 cut(s) 185, 260, 352, 499, 518, 598
MboII GAAGA 1 cut(s) 150
MfeI CAATTG 1 cut(s) 618
MflI RGATCY 1 cut(s) 185
MlsI TGGCCA 1 cut(s) 459
MluCI AATT 4 cut(s) 194, 429, 618, 672
MluNI TGGCCA 1 cut(s) 459
Mly113I GGCGCC 1 cut(s) 587
MlyI GAGTC 1 cut(s) 13
MmeI TCCRAC 1 cut(s) 288
MnlI CCTC 2 cut(s) 242, 457
Mox20I TGGCCA 1 cut(s) 459
MscI TGGCCA 1 cut(s) 459
MseI TTAA 1 cut(s) 685
Msp20I TGGCCA 1 cut(s) 459
MspA1I CMGCKG 3 cut(s) 387, 425, 475
MspR9I CCNGG 1 cut(s) 370
MunI CAATTG 1 cut(s) 618
MvaI CCWGG 1 cut(s) 370
MvnI CGCG 1 cut(s) 259
MwoI GCNNNNNNNGC 5 cut(s) 313, 530, 539, 593, 621
NarI GGCGCC 1 cut(s) 587
NdeII GATC 6 cut(s) 185, 260, 352, 499, 518, 598
NlaIV GGNNCC 2 cut(s) 375, 588
NmuCI GTSAC 1 cut(s) 573
NsbI TGCGCA 1 cut(s) 628
PkrI GCNGC 8 cut(s) 181, 290, 386, 424, 494, 591, 614, 626
Ple19I CGATCG 1 cut(s) 502
PleI GAGTC 1 cut(s) 12
PluTI GGCGCC 1 cut(s) 590
PmaCI CACGTG 1 cut(s) 463
PmlI CACGTG 1 cut(s) 463
PpsI GAGTC 1 cut(s) 12
Ppu21I YACGTR 1 cut(s) 463
PpuMI RGGWCCY 1 cut(s) 366
Psp5II RGGWCCY 1 cut(s) 366
Psp6I CCWGG 1 cut(s) 368
PspCI CACGTG 1 cut(s) 463
PspEI GGTNACC 1 cut(s) 573
PspGI CCWGG 1 cut(s) 368
PspN4I GGNNCC 2 cut(s) 375, 588
PspPI GGNCC 1 cut(s) 366
PspPPI RGGWCCY 1 cut(s) 366
PstNI CAGNNNCTG 2 cut(s) 369, 401
PsuI RGATCY 1 cut(s) 185
PvuI CGATCG 1 cut(s) 502
RsaI GTAC 2 cut(s) 241, 659
RsaNI GTAC 2 cut(s) 240, 658
SaqAI TTAA 1 cut(s) 685
SatI GCNGC 8 cut(s) 180, 289, 385, 423, 493, 590, 613, 625
Sau3AI GATC 6 cut(s) 185, 260, 352, 499, 518, 598
Sau96I GGNCC 1 cut(s) 366
SchI GAGTC 1 cut(s) 13
ScrFI CCNGG 1 cut(s) 370
SetI ASST 4 cut(s) 368, 400, 449, 465
SfaNI GCATC 4 cut(s) 303, 456, 542, 545
SfoI GGCGCC 1 cut(s) 588
SinI GGWCC 1 cut(s) 366
Sse9I AATT 4 cut(s) 194, 429, 618, 672
SsiI CCGC 6 cut(s) 327, 387, 425, 475, 558, 590
SspDI GGCGCC 1 cut(s) 586
StyD4I CCNGG 1 cut(s) 368
TaaI ACNGT 3 cut(s) 296, 607, 657
TaiI ACGT 1 cut(s) 465
TaqI TCGA 1 cut(s) 284
TasI AATT 4 cut(s) 194, 429, 618, 672
TatI WGTACW 1 cut(s) 239
TauI GCSGC 1 cut(s) 592
Tru1I TTAA 1 cut(s) 685
Tru9I TTAA 1 cut(s) 685
TscAI CASTG 1 cut(s) 314
TseFI GTSAC 1 cut(s) 573
TseI GCWGC 7 cut(s) 179, 288, 384, 422, 492, 612, 624
Tsp45I GTSAC 1 cut(s) 573
TspDTI ATGAA 2 cut(s) 359, 579
TspRI CASTG 1 cut(s) 314
VpaK11BI GGWCC 1 cut(s) 366
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.