pycom16g17970

HR-like lesion-inducing

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0011782
Physical Location & Seq
Reverse (-)
14337048 .. 14338961
1914 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 477 bp
ATGGGGTTCGTCTCCTTCCTCGGCCGCGTCCTCTTCGCCTCCATCTTCATGCTCTCTGCTTGGCAAGCGTTTAATGAATTTGGTGTTGATGGTGGCCCTGCTGCAAAGGACTTGGCTCCCAAGTTTAATGTGTTCAAGAAAAATTTGTCCGCTAAGCTAGGGGTTGCAATACCGGAGATAGAGGTAAGACATTTAGCTGCTGGTGTGGTAGCAATGAAAGGGCTTGGAGGACTTCTATTCGTGCTTAACAGTTCCTTTGGAGCTTCTCTCCTGCTTCTACAATTGGCAATTACCACTCCCCTTATCTATGATTTCTACAACTACAGTCCTGAGAACGCCAAATTTGGCATACTCCTAAACGAATTCTTGCAGCACGCAGCGCTTTTTGGTGCACTGCTATTCTTCATTGGCATGAAGAACTCAATTCCAAGGAGGCAAGTCAAAAAGAAGGCTGCCCCAAAAACCAAAACGGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HR_lesion

Protein Analysis

159

Amino Acids

17.13

Weight (kDa)

10.0

Isoelectric Point (pI)

33.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HR_lesion PF05514 1 - 138 3e-57 HR-like lesion-inducing
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011782)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G04340 AT5G43460 AT5G43460
fragaria_vesca FvH4_4g03211 FvH4_5g30450
malus_domestica MD13G1210700.v1.1 MD16G1213900.v1.1
prunus_persica Prupe.1G035100_v2.0.a1
pyrus_communis pycom13g18320 pycom16g17970
rosa_chinensis RchiOBHm_Chr4g0391891
rosa_laevigata RLG00000009851
rosa_multiflora Rmu_co8463711.1_g000001 Rmu_sc0000383.1_g000013 Rmu_sc0007693.1_g000011
rosa_rugosa Rorug03G0336700
rosa_samantha Rh4AG039100 Rh4BG033800
rosa_wichuraiana Rw4G003050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 27
AciI CCGC 2 cut(s) 25, 150
AcoI YGGCCR 1 cut(s) 22
AcsI RAATTY 4 cut(s) 77, 142, 341, 362
AfeI AGCGCT 1 cut(s) 381
AgsI TTSAA 1 cut(s) 136
AluBI AGCT 3 cut(s) 157, 197, 263
AluI AGCT 3 cut(s) 157, 197, 263
Alw21I GWGCWC 1 cut(s) 394
Alw26I GTCTC 1 cut(s) 16
Alw44I GTGCAC 1 cut(s) 390
Aor51HI AGCGCT 1 cut(s) 381
AoxI GGCC 2 cut(s) 22, 94
ApaLI GTGCAC 1 cut(s) 390
ApeKI GCWGC 5 cut(s) 101, 197, 370, 377, 452
ApoI RAATTY 4 cut(s) 77, 142, 341, 362
AspLEI GCGC 1 cut(s) 382
AspS9I GGNCC 1 cut(s) 95
BaeGI GKGCMC 1 cut(s) 394
Bbv12I GWGCWC 1 cut(s) 394
BbvI GCAGC 5 cut(s) 88, 184, 382, 389, 439
BccI CCATC 2 cut(s) 50, 83
BcoDI GTCTC 1 cut(s) 16
BfaI CTAG 1 cut(s) 158
BfmI CTRYAG 1 cut(s) 322
BfoI RGCGCY 1 cut(s) 383
BisI GCNGC 6 cut(s) 25, 102, 198, 371, 378, 453
BlpI GCTNAGC 1 cut(s) 153
BlsI GCNGC 6 cut(s) 26, 103, 199, 372, 379, 454
BmgT120I GGNCC 1 cut(s) 95
BmiI GGNNCC 1 cut(s) 117
BplI GAGNNNNNCTC 2 cut(s) 252, 284
Bpu1102I GCTNAGC 1 cut(s) 153
BsaJI CCNNGG 2 cut(s) 19, 428
BsaWI WCCGGW 1 cut(s) 172
Bse3DI GCAATG 1 cut(s) 219
BseDI CCNNGG 2 cut(s) 19, 428
BseMI GCAATG 1 cut(s) 219
BseMII CTCAG 1 cut(s) 321
BseSI GKGCMC 1 cut(s) 394
BseX3I CGGCCG 1 cut(s) 22
BseXI GCAGC 5 cut(s) 88, 184, 382, 389, 439
Bsh1236I CGCG 1 cut(s) 27
Bsh1285I CGRYCG 1 cut(s) 25
BshFI GGCC 2 cut(s) 24, 96
BsiEI CGRYCG 1 cut(s) 25
BsiHKAI GWGCWC 1 cut(s) 394
BsiSI CCGG 1 cut(s) 173
BsmAI GTCTC 1 cut(s) 16
BsmBI CGTCTC 1 cut(s) 16
BsnI GGCC 2 cut(s) 24, 96
Bsp1286I GDGCHC 1 cut(s) 394
Bsp1720I GCTNAGC 1 cut(s) 153
BspACI CCGC 2 cut(s) 25, 150
BspANI GGCC 2 cut(s) 24, 96
BspCNI CTCAG 1 cut(s) 322
BspFNI CGCG 1 cut(s) 27
BspLI GGNNCC 1 cut(s) 117
BsrDI GCAATG 1 cut(s) 219
BssECI CCNNGG 2 cut(s) 19, 428
BssT1I CCWWGG 1 cut(s) 428
Bst4CI ACNGT 2 cut(s) 251, 326
Bst6I CTCTTC 1 cut(s) 38
BstC8I GCNNGC 2 cut(s) 66, 375
BstDEI CTNAG 2 cut(s) 153, 330
BstFNI CGCG 1 cut(s) 27
BstH2I RGCGCY 1 cut(s) 383
BstHHI GCGC 1 cut(s) 382
BstMAI GTCTC 1 cut(s) 16
BstMCI CGRYCG 1 cut(s) 25
BstMWI GCNNNNNNNGC 2 cut(s) 65, 379
BstSFI CTRYAG 1 cut(s) 322
BstSLI GKGCMC 1 cut(s) 394
BstUI CGCG 1 cut(s) 27
BstV1I GCAGC 5 cut(s) 88, 184, 382, 389, 439
BstZI CGGCCG 1 cut(s) 22
BsuRI GGCC 2 cut(s) 24, 96
BtsI GCAGTG 1 cut(s) 392
BtsIMutI CAGTG 1 cut(s) 392
Cac8I GCNNGC 2 cut(s) 66, 375
CfoI GCGC 1 cut(s) 382
Cfr13I GGNCC 1 cut(s) 95
CseI GACGC 1 cut(s) 16
CviAII CATG 2 cut(s) 49, 412
CviJI RGCY 8 cut(s) 24, 96, 116, 157, 197, 223, 263, 452
CviKI_1 RGCY 8 cut(s) 24, 96, 116, 157, 197, 223, 263, 452
DdeI CTNAG 2 cut(s) 153, 330
EaeI YGGCCR 1 cut(s) 22
EagI CGGCCG 1 cut(s) 22
Eam1104I CTCTTC 1 cut(s) 38
EarI CTCTTC 1 cut(s) 38
EclXI CGGCCG 1 cut(s) 22
Eco130I CCWWGG 1 cut(s) 428
Eco47III AGCGCT 1 cut(s) 381
Eco52I CGGCCG 1 cut(s) 22
EcoRI GAATTC 1 cut(s) 362
EcoT14I CCWWGG 1 cut(s) 428
ErhI CCWWGG 1 cut(s) 428
Esp3I CGTCTC 1 cut(s) 16
FaeI CATG 2 cut(s) 52, 415
FaiI YATR 4 cut(s) 50, 309, 350, 413
FatI CATG 2 cut(s) 48, 411
Fnu4HI GCNGC 6 cut(s) 25, 102, 198, 371, 378, 453
Fsp4HI GCNGC 6 cut(s) 25, 102, 198, 371, 378, 453
FspBI CTAG 1 cut(s) 158
GlaI GCGC 1 cut(s) 381
GluI GCNGC 6 cut(s) 25, 102, 198, 371, 378, 453
HaeII RGCGCY 1 cut(s) 383
HaeIII GGCC 2 cut(s) 24, 96
HapII CCGG 1 cut(s) 173
HgaI GACGC 1 cut(s) 16
HhaI GCGC 1 cut(s) 382
Hin1II CATG 2 cut(s) 52, 415
Hin6I GCGC 1 cut(s) 380
HinP1I GCGC 1 cut(s) 380
HpaII CCGG 1 cut(s) 173
Hpy166II GTNNAC 1 cut(s) 392
Hpy188III TCNNGA 2 cut(s) 136, 329
Hpy8I GTNNAC 1 cut(s) 392
HpyAV CCTTC 2 cut(s) 25, 442
HpyCH4III ACNGT 2 cut(s) 251, 326
HpyCH4V TGCA 4 cut(s) 104, 167, 370, 392
HpyF10VI GCNNNNNNNGC 2 cut(s) 65, 379
HpyF3I CTNAG 2 cut(s) 153, 330
Hsp92II CATG 2 cut(s) 52, 415
HspAI GCGC 1 cut(s) 380
LmnI GCTCC 2 cut(s) 121, 260
LpnPI CCDG 5 cut(s) 111, 186, 186, 284, 342
Lsp1109I GCAGC 5 cut(s) 88, 184, 382, 389, 439
MaeI CTAG 1 cut(s) 158
MboII GAAGA 4 cut(s) 25, 37, 394, 427
MfeI CAATTG 1 cut(s) 281
MhlI GDGCHC 1 cut(s) 394
MluCI AATT 7 cut(s) 77, 142, 281, 288, 341, 362, 423
MnlI CCTC 6 cut(s) 29, 41, 49, 175, 221, 426
MseI TTAA 3 cut(s) 72, 126, 246
MslI CAYNNNNRTG 2 cut(s) 47, 410
MspI CCGG 1 cut(s) 173
MunI CAATTG 1 cut(s) 281
MvnI CGCG 1 cut(s) 27
MwoI GCNNNNNNNGC 2 cut(s) 65, 379
NlaIII CATG 2 cut(s) 52, 415
NlaIV GGNNCC 1 cut(s) 117
PkrI GCNGC 6 cut(s) 26, 103, 199, 372, 379, 454
PspN4I GGNNCC 1 cut(s) 117
PspPI GGNCC 1 cut(s) 95
RseI CAYNNNNRTG 2 cut(s) 47, 410
SaqAI TTAA 3 cut(s) 72, 126, 246
SatI GCNGC 6 cut(s) 25, 102, 198, 371, 378, 453
Sau96I GGNCC 1 cut(s) 95
SduI GDGCHC 1 cut(s) 394
SetI ASST 4 cut(s) 159, 186, 199, 265
SfcI CTRYAG 1 cut(s) 322
SmiMI CAYNNNNRTG 2 cut(s) 47, 410
Sse9I AATT 7 cut(s) 77, 142, 281, 288, 341, 362, 423
SsiI CCGC 2 cut(s) 25, 150
SspMI CTAG 1 cut(s) 158
StyI CCWWGG 1 cut(s) 428
TaaI ACNGT 2 cut(s) 251, 326
TasI AATT 7 cut(s) 77, 142, 281, 288, 341, 362, 423
TauI GCSGC 1 cut(s) 27
Tru1I TTAA 3 cut(s) 72, 126, 246
Tru9I TTAA 3 cut(s) 72, 126, 246
TscAI CASTG 1 cut(s) 399
TseI GCWGC 5 cut(s) 101, 197, 370, 377, 452
TspDTI ATGAA 5 cut(s) 37, 90, 230, 394, 428
TspRI CASTG 1 cut(s) 399
VneI GTGCAC 1 cut(s) 390
XapI RAATTY 4 cut(s) 77, 142, 341, 362
XspI CTAG 1 cut(s) 158
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.