pycom16g20430

E2F-associated phosphoprotein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0014441
Physical Location & Seq
Reverse (-)
18212569 .. 18215944
3376 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 648 bp
ATGATATGGTTTTGTGCAACTCCTTCCAGAATCCAGCCGAGCCCAGATAATGCTGGGTGGGCTCATCAAAATATCAGCAACCCAAATACAACCGCCATAAATAAAACTAAACAAATTAAAACAAAGCAAAGGGGAAAGAGGCGAGACAGAGTCTTAAACCCTGGACAGATGGAGATGGACAAGATGACAGACCACATGGCCTCTCCATCCAATTCTCAGAAAACTGTTTCTGATGATGATGAAATTGACTACTCCGTCAAACCAGAGTTCTATGATTCAGATATTGATTACAAGGATGAGTCATGGGTTCAAAAGAAAAGGAAAGGTGGCACTTCTGATGCTGTGCTTAGTTGTCCATCTTGTTTCACCACGCTCTGCCTTGACTGTCAAAGGCATGAAAAATATGTGACCCAGTACAGAGCAATTTTTGTTGAGAACTGCAAGATTGGAAGTGATCAAATAGTGCATCAAAAAGAAGCAAGACCTAGAAGGGGCAAAAGAGGGAGAGAATCTTCGGAGCATGAAGCAACTCCTGCAGGTAACCAGACCGTCAAGCAAGTTTGCTGCTTAGTTTGCTCAACTGAGGTAGGTGTAATCGACGCTGATGAAGTTTATCATTTCTTCAATGTTCTTCCAAGCGAGTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Eapp_C

Protein Analysis

216

Amino Acids

24.4

Weight (kDa)

6.98

Isoelectric Point (pI)

48.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Eapp_C PF10238 90 - 213 5.3e-48 E2F-associated phosphoprotein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014441)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G08320
fragaria_vesca FvH4_4g06750
malus_domestica MD16G1243400.v1.1
prunus_persica Prupe.1G070600_v2.0.a1
pyrus_communis pycom16g20430
rosa_chinensis RchiOBHm_Chr4g0398681
rosa_laevigata RLG00000009333
rosa_multiflora Rmu_sc0000586.1_g000015 Rmu_sc0008600.1_g000024
rosa_roxburghii Rroxscaffold_5G00343630
rosa_rugosa Rorug04G0010700
rosa_samantha Rh4AG088300 Rh4BG085000 Rh4BG085200 Rh4CG096500 Rh4DG080000
rosa_wichuraiana Rw4G007320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 254
Acc36I ACCTGC 1 cut(s) 527
AciI CCGC 1 cut(s) 93
AfaI GTAC 1 cut(s) 416
AfiI CCNNNNNNNGG 1 cut(s) 491
AgsI TTSAA 2 cut(s) 311, 625
AjnI CCWGG 1 cut(s) 160
Alw26I GTCTC 1 cut(s) 138
AoxI GGCC 1 cut(s) 198
ApeKI GCWGC 1 cut(s) 564
AsuHPI GGTGA 1 cut(s) 358
BanII GRGCYC 2 cut(s) 44, 64
BbvI GCAGC 1 cut(s) 551
BccI CCATC 4 cut(s) 163, 169, 214, 364
BciT130I CCWGG 1 cut(s) 162
BclI TGATCA 1 cut(s) 454
BcoDI GTCTC 1 cut(s) 138
BfaI CTAG 1 cut(s) 486
BfmI CTRYAG 1 cut(s) 534
BfuAI ACCTGC 1 cut(s) 527
BisI GCNGC 1 cut(s) 565
BlsI GCNGC 1 cut(s) 566
Bme1390I CCNGG 1 cut(s) 162
BmrFI CCNGG 1 cut(s) 162
BmrI ACTGGG 1 cut(s) 406
BmsI GCATC 2 cut(s) 328, 475
BmuI ACTGGG 1 cut(s) 406
BsaJI CCNNGG 1 cut(s) 160
Bsc4I CCNNNNNNNGG 1 cut(s) 491
Bse1I ACTGG 1 cut(s) 412
BseBI CCWGG 1 cut(s) 162
BseDI CCNNGG 1 cut(s) 160
BseGI GGATG 2 cut(s) 206, 301
BseLI CCNNNNNNNGG 1 cut(s) 491
BseMII CTCAG 2 cut(s) 230, 573
BseNI ACTGG 1 cut(s) 412
BseXI GCAGC 1 cut(s) 551
BseYI CCCAGC 1 cut(s) 53
BshFI GGCC 1 cut(s) 200
BslI CCNNNNNNNGG 1 cut(s) 491
BsmAI GTCTC 1 cut(s) 138
BsnI GGCC 1 cut(s) 200
Bsp1286I GDGCHC 2 cut(s) 44, 64
Bsp143I GATC 1 cut(s) 454
BspACI CCGC 1 cut(s) 93
BspANI GGCC 1 cut(s) 200
BspCNI CTCAG 2 cut(s) 229, 574
BspMAI CTGCAG 1 cut(s) 538
BspMI ACCTGC 1 cut(s) 527
BsrI ACTGG 1 cut(s) 412
BssECI CCNNGG 1 cut(s) 160
BssMI GATC 1 cut(s) 454
Bst2UI CCWGG 1 cut(s) 162
Bst4CI ACNGT 3 cut(s) 226, 386, 550
BstAPI GCANNNNNTGC 1 cut(s) 533
BstDEI CTNAG 4 cut(s) 216, 347, 568, 582
BstEII GGTNACC 1 cut(s) 539
BstF5I GGATG 2 cut(s) 206, 301
BstKTI GATC 1 cut(s) 457
BstMAI GTCTC 1 cut(s) 138
BstMBI GATC 1 cut(s) 454
BstMWI GCNNNNNNNGC 3 cut(s) 59, 533, 573
BstNI CCWGG 1 cut(s) 162
BstPI GGTNACC 1 cut(s) 539
BstSCI CCNGG 1 cut(s) 160
BstSFI CTRYAG 1 cut(s) 534
BstV1I GCAGC 1 cut(s) 551
BsuRI GGCC 1 cut(s) 200
BtsCI GGATG 2 cut(s) 206, 301
BveI ACCTGC 1 cut(s) 527
CseI GACGC 1 cut(s) 608
Csp6I GTAC 1 cut(s) 415
CviAII CATG 4 cut(s) 196, 303, 395, 521
CviJI RGCY 4 cut(s) 37, 42, 62, 200
CviKI_1 RGCY 4 cut(s) 37, 42, 62, 200
CviQI GTAC 1 cut(s) 415
DdeI CTNAG 4 cut(s) 216, 347, 568, 582
DpnI GATC 1 cut(s) 456
DpnII GATC 1 cut(s) 454
DrdI GACNNNNNNGTC 1 cut(s) 254
DseDI GACNNNNNNGTC 1 cut(s) 254
Eco24I GRGCYC 2 cut(s) 44, 64
Eco91I GGTNACC 1 cut(s) 539
EcoO65I GGTNACC 1 cut(s) 539
EcoRII CCWGG 1 cut(s) 160
EcoT38I GRGCYC 2 cut(s) 44, 64
FaeI CATG 4 cut(s) 199, 306, 398, 524
FaiI YATR 8 cut(s) 7, 98, 197, 273, 304, 396, 405, 522
FalI AAGNNNNNCTT 1 cut(s) 628
FatI CATG 4 cut(s) 195, 302, 394, 520
FbaI TGATCA 1 cut(s) 454
Fnu4HI GCNGC 1 cut(s) 565
FokI GGATG 2 cut(s) 193, 308
FriOI GRGCYC 2 cut(s) 44, 64
Fsp4HI GCNGC 1 cut(s) 565
FspBI CTAG 1 cut(s) 486
GluI GCNGC 1 cut(s) 565
GsaI CCCAGC 1 cut(s) 57
HaeIII GGCC 1 cut(s) 200
HgaI GACGC 1 cut(s) 608
Hin1II CATG 4 cut(s) 199, 306, 398, 524
HinfI GANTC 6 cut(s) 30, 150, 275, 299, 509, 641
HphI GGTGA 1 cut(s) 358
Hpy188I TCNGA 5 cut(s) 219, 232, 280, 337, 517
Hpy188III TCNNGA 2 cut(s) 27, 645
Hpy99I CGWCG 1 cut(s) 602
HpyAV CCTTC 2 cut(s) 33, 483
HpyCH4III ACNGT 3 cut(s) 226, 386, 550
HpyCH4V TGCA 4 cut(s) 17, 441, 466, 536
HpyF10VI GCNNNNNNNGC 3 cut(s) 59, 533, 573
HpyF3I CTNAG 4 cut(s) 216, 347, 568, 582
Hsp92II CATG 4 cut(s) 199, 306, 398, 524
Ksp22I TGATCA 1 cut(s) 454
Kzo9I GATC 1 cut(s) 454
LmnI GCTCC 1 cut(s) 517
Lsp1109I GCAGC 1 cut(s) 551
LweI GCATC 2 cut(s) 328, 475
MaeI CTAG 1 cut(s) 486
MaeIII GTNAC 2 cut(s) 406, 539
MalI GATC 1 cut(s) 456
MboI GATC 1 cut(s) 454
MboII GAAGA 3 cut(s) 504, 613, 623
MhlI GDGCHC 2 cut(s) 44, 64
MluCI AATT 4 cut(s) 114, 211, 243, 423
MlyI GAGTC 2 cut(s) 159, 308
MnlI CCTC 4 cut(s) 132, 211, 494, 577
MseI TTAA 2 cut(s) 117, 155
MspR9I CCNGG 1 cut(s) 162
MvaI CCWGG 1 cut(s) 162
MwoI GCNNNNNNNGC 3 cut(s) 59, 533, 573
NdeII GATC 1 cut(s) 454
NlaIII CATG 4 cut(s) 199, 306, 398, 524
NmeAIII GCCGAG 1 cut(s) 63
NmuCI GTSAC 1 cut(s) 406
PfeI GAWTC 3 cut(s) 30, 275, 509
PflFI GACNNNGTC 1 cut(s) 149
PkrI GCNGC 1 cut(s) 566
PleI GAGTC 2 cut(s) 158, 307
PpsI GAGTC 2 cut(s) 158, 307
Psp6I CCWGG 1 cut(s) 160
PspEI GGTNACC 1 cut(s) 539
PspFI CCCAGC 1 cut(s) 53
PspGI CCWGG 1 cut(s) 160
PstI CTGCAG 1 cut(s) 538
PsyI GACNNNGTC 1 cut(s) 149
RsaI GTAC 1 cut(s) 416
RsaNI GTAC 1 cut(s) 415
SaqAI TTAA 2 cut(s) 117, 155
SatI GCNGC 1 cut(s) 565
Sau3AI GATC 1 cut(s) 454
SbfI CCTGCAGG 1 cut(s) 538
SchI GAGTC 2 cut(s) 159, 308
ScrFI CCNGG 1 cut(s) 162
SdaI CCTGCAGG 1 cut(s) 538
SduI GDGCHC 2 cut(s) 44, 64
SetI ASST 5 cut(s) 328, 487, 541, 588, 592
SfaNI GCATC 2 cut(s) 328, 475
SfcI CTRYAG 1 cut(s) 534
Sse8387I CCTGCAGG 1 cut(s) 538
Sse9I AATT 4 cut(s) 114, 211, 243, 423
SsiI CCGC 1 cut(s) 93
SspMI CTAG 1 cut(s) 486
StyD4I CCNGG 1 cut(s) 160
TaaI ACNGT 3 cut(s) 226, 386, 550
TaqI TCGA 1 cut(s) 597
TasI AATT 4 cut(s) 114, 211, 243, 423
TatI WGTACW 1 cut(s) 414
TfiI GAWTC 3 cut(s) 30, 275, 509
Tru1I TTAA 2 cut(s) 117, 155
Tru9I TTAA 2 cut(s) 117, 155
TseFI GTSAC 1 cut(s) 406
TseI GCWGC 1 cut(s) 564
Tsp45I GTSAC 1 cut(s) 406
TspDTI ATGAA 4 cut(s) 255, 411, 537, 621
TspGWI ACGGA 1 cut(s) 244
Tth111I GACNNNGTC 1 cut(s) 149
XspI CTAG 1 cut(s) 486
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.