Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr16

Orthogroup ID

OG0006848

Physical Location & Seq

Genomic Coordinates Forward (+)

18366368 .. 18370446

Gene Structure 4079 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1899 bp

ATGATTACAGGCAAGGACATTTACGATGTTCTTGCAGCCATAGTCCCCTTATACGTGGCTATGATCTTAGCCTACGGTTCCGTTAGGTGGTGGAAAATCTTCACGCCCGACCAATGCTCCGGCATAAACCGCTTCGTCGCGGTTTTCGCCGTCCCATTGCTCTCCTTCCACTTCATATCCTCCAACGACCCTTACGCCATGAACTACCAATTCATCCTCGCCGACTCCCTCCAAAAAGTCGTTATCCTCGTTGCCCTCTTCCTCTGGCAAGCCTTCTCCAAGCGCGGCAACCTCGAGTGGATGATCACGCTTTTCTCCCTCTCCACCCTCCCCAACACCCTCGTCATGGGCATCCCTCTTTTACGCGCCATGTATGGCGACTTCTCCGGGACGCTGATGGTTCAGATTGTCGTTTTGCAGAGCGTGGTCTGGTACACTCTCATGCTCTTCATGTTCGAGTACCGCGGCGCGAAGCTGCTCATCTCCGAGCAGTTCCCGGAGACTGCAGCTTCAATCACGTCGTTCAAAGTTGACTCTGACGTCGTTTCGCTCAACGGTCGAGAGCCGCTGCAAACCGACGCGGAAATCGGCGATGACGGGAAGCTTCACGTGGTGGTGAAAAGATCGAATGCGTCGTCTATGGTATCTTCCTTCAACAAGTCACACGGGTTGAACTCTTTGAATTCGATGACCCCACGGGCTTCGAATCTCACGGGAGTCGAGATTTACTCGGTGAAGTCCTCTCGTGAGCCCACCCCGCGGGCTTCCAGCTTCAACCAGACGGATTTTTACGCCATGTTTAACGCCAGCAAGGCCCCAAGCCCCAAACACGGATATACAAATAGTTTTCAAGGCGGATTCGGGGACGTTTATTCGGTGCAGTCATCCAAAGGGGTGACGCCGAGAACTTCGAATTTCGATGAGGAGGCGATGAAGATGAATAATATGAGTTATAAGAAGAGCGGGGCGAGGAGCATGAGTGGTGAGATCTTCAATGGTGGTCCGGCCTCTCCGTACCCGCCTCCAAATCCCTTGTTTTCAGGGTCTACGAGTGGCGGGCTGAAGAAGAAAGATAGCGCTAGTGGTGGCGGTGGTGGTAGCGGTGGGGCAGCGCCTAATAAGGAGCCTCATATGTTTGTTTGCAGTTCAAATGCATCGCCAGTTTCCGAAGGGAATCTTAGACTTGCGGTTGCTTCCACTGACTTTGGCGCGACCGATCCTTCCAAGGTTGCAGGTCTTCATCAACATGATAGTGCTGCTGCATCGAAAGGTATACACGAATTAATTGAGAATATGAGTCCTGGGAGGAAAATGAGAGGAGATAGCGAGCTTGAGATAGAAGAAGGATCGAATAAATTTCCACCGAGTGTGTCATCGTACAGTAGTAGTTGCCAAAAGAAGGTTGATATGGAAGGAGGTGTAGCAAAGAAGCACCAAATGCCTCCAGCAAGTGTCATGACTAGGCTTATTCTCATCATGGTCTGGAGAAAGCTCATTAGAAACCCCAACACTTACTCCAGCCTTCTTGGCGTGGCATGGTCTCTCATATCATACAAGTACATCTCTCTCTCTCTCTACAGTGAGTGCACCCACCATGCATTGTGGAAAATCAAAATGCCAACAATTGTAAGTGGATCCATATCAATATTATCTGATGCTGGCTTAGGAATGGCTATGTTCAGTCTTGGATTATTCATGGCCTTACAACCAAAGATCATAGCTTGTGGAAAATCTGTTGCAACATTTTCAATGGCTGTTAGGTTCTTAACAGGCCCAGCAGTGATTGCTGCAACTTCTATTGCTGTCGGTCTTCGTGGAGTTCTTTTGCATGTAGCAATAGTTCAGGTTCGCCAACTCCACCTTTGCAACCATCCCCACATTTCTAAAATCTTATGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000323 GO:0001666 GO:0003674 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005783 GO:0005886 GO:0006810 GO:0006950 GO:0008150 GO:0009605 GO:0009606 GO:0009628 GO:0009629 GO:0009630 GO:0009719 GO:0009723 GO:0009725 GO:0009733 GO:0009743 GO:0009746 GO:0009749 GO:0009914 GO:0009921 GO:0009925 GO:0009926 GO:0009958 GO:0009966 GO:0010033 GO:0010252 GO:0010315 GO:0010329 GO:0010646 GO:0010817 GO:0010928 GO:0012505 GO:0015562 GO:0016020 GO:0016323 GO:0022857 GO:0023051 GO:0032991 GO:0034284 GO:0036293 GO:0042221 GO:0042592 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044425 GO:0044444 GO:0044459 GO:0044464 GO:0045178 GO:0048583 GO:0048878 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0055085 GO:0060918 GO:0065007 GO:0065008 GO:0070482 GO:0071944 GO:0080161 GO:0098590 GO:0098796 GO:0098797 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Mem_trans

Protein Analysis

633

Amino Acids

68.39

Weight (kDa)

9.27

Isoelectric Point (pI)

44.12

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Mem_trans	PF03547	10 - 615	1.4e-98	Membrane transport protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0006848)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G57090 AT5G57090
fragaria_vesca	FvH4_4g06870
malus_domestica	MD13G1239200.v1.1 MD16G1244500.v1.1
prunus_persica	Prupe.1G071700_v2.0.a1 Prupe.1G071800_v2.0.a1
pyrus_communis	pycom16g20590
rosa_chinensis	RchiOBHm_Chr4g0398751 RchiOBHm_Chr4g0398781
rosa_laevigata	RLG00000009323 RLG00000009324
rosa_multiflora	Rmu_sc0000458.1_g000007
rosa_roxburghii	Rroxscaffold_5G00343700 Rroxscaffold_5G00343710
rosa_rugosa	Rorug04G0011300 Rorug04G0011400
rosa_samantha	Rh4AG089200 Rh4AG089300 Rh4BG086600 Rh4CG097200 Rh4CG097400 Rh4DG080900 Rh4DG081100
rosa_wichuraiana	Rw4G007370 Rw4G007380

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	954
AasI	GACNNNNNNGTC	1 cut(s)	539
AatII	GACGTC	1 cut(s)	543
Acc36I	ACCTGC	1 cut(s)	1223
AccBSI	CCGCTC	1 cut(s)	963
AccI	GTMKAC	2 cut(s)	1046, 1273
AccII	CGCG	8 cut(s)	140, 285, 366, 465, 470, 581, 760, 1211
AclWI	GGATC	4 cut(s)	1211, 1354, 1629, 1642
AcsI	RAATTY	3 cut(s)	682, 913, 1355
AcuI	CTGAAG	1 cut(s)	1082
AcvI	CACGTG	1 cut(s)	610
AcyI	GRCGYC	2 cut(s)	540, 899
AdeI	CACNNNGTG	2 cut(s)	613, 1367
AfaI	GTAC	5 cut(s)	434, 461, 1016, 1379, 1559
AfeI	AGCGCT	1 cut(s)	1078
AfiI	CCNNNNNNNGG	5 cut(s)	87, 346, 759, 830, 1399
AjiI	CACGTC	1 cut(s)	519
AjnI	CCWGG	1 cut(s)	1300
AluBI	AGCT	7 cut(s)	475, 509, 604, 771, 1330, 1492, 1721
AluI	AGCT	7 cut(s)	475, 509, 604, 771, 1330, 1492, 1721
Alw21I	GWGCWC	1 cut(s)	1589
Alw26I	GTCTC	2 cut(s)	494, 1545
Alw44I	GTGCAC	1 cut(s)	1585
AlwI	GGATC	4 cut(s)	1211, 1354, 1629, 1642
Ama87I	CYCGRG	1 cut(s)	293
Aor51HI	AGCGCT	1 cut(s)	1078
AoxI	GGCC	4 cut(s)	813, 1005, 1698, 1771
ApaLI	GTGCAC	1 cut(s)	1585
ApeKI	GCWGC	8 cut(s)	35, 475, 506, 568, 1109, 1256, 1259, 1787
ApoI	RAATTY	3 cut(s)	682, 913, 1355
ArsI	GACNNNNNNTTYG	2 cut(s)	620, 652
AseI	ATTAAT	1 cut(s)	1283
Asp700I	GAANNNNTTC	1 cut(s)	98
AspLEI	GCGC	6 cut(s)	285, 368, 470, 1079, 1114, 1211
AspS9I	GGNCC	3 cut(s)	814, 1001, 1772
AsuC2I	CCSGG	2 cut(s)	388, 497
AsuHPI	GGTGA	4 cut(s)	628, 745, 907, 995
AsuII	TTCGAA	2 cut(s)	704, 911
AvaI	CYCGRG	1 cut(s)	293
AvaII	GGWCC	1 cut(s)	1001
BaeGI	GKGCMC	1 cut(s)	1589
BamHI	GGATCC	1 cut(s)	1634
BanII	GRGCYC	1 cut(s)	753
BarI	GAAGNNNNNNTAC	2 cut(s)	365, 397
BauI	CACGAG	1 cut(s)	744
BbrPI	CACGTG	1 cut(s)	610
BbsI	GAAGAC	2 cut(s)	1229, 1802
Bbv12I	GWGCWC	1 cut(s)	1589
BbvI	GCAGC	8 cut(s)	47, 462, 518, 555, 1121, 1243, 1246, 1774
BccI	CCATC	2 cut(s)	391, 1878
BceAI	ACGGC	1 cut(s)	134
BcgI	CGANNNNNNTGC	2 cut(s)	14, 48
BciT130I	CCWGG	1 cut(s)	1302
BclI	TGATCA	1 cut(s)	303
BcnI	CCSGG	2 cut(s)	388, 497
BcoDI	GTCTC	2 cut(s)	494, 1545
BfaI	CTAG	2 cut(s)	1080, 1461
BfmI	CTRYAG	2 cut(s)	504, 1576
BfoI	RGCGCY	2 cut(s)	1080, 1115
BfuAI	ACCTGC	1 cut(s)	1223
BglI	GCCNNNNNGGC	2 cut(s)	812, 1527
BglII	AGATCT	1 cut(s)	987
Bme1390I	CCNGG	3 cut(s)	388, 497, 1302
Bme18I	GGWCC	1 cut(s)	1001
BmeT110I	CYCGRG	1 cut(s)	293
BmgBI	CACGTC	1 cut(s)	519
BmgT120I	GGNCC	3 cut(s)	814, 1001, 1772
BmiI	GGNNCC	4 cut(s)	79, 816, 1125, 1636
BmrFI	CCNGG	3 cut(s)	388, 497, 1302
BmsI	GCATC	4 cut(s)	360, 1163, 1271, 1645
BpiI	GAAGAC	2 cut(s)	1229, 1802
BplI	GAGNNNNNCTC	2 cut(s)	713, 745
BpmI	CTGGAG	3 cut(s)	1428, 1501, 1504
Bpu10I	CCTNAGC	1 cut(s)	1663
Bpu14I	TTCGAA	2 cut(s)	704, 911
BpuEI	CTTGAG	1 cut(s)	1352
BpuMI	CCSGG	2 cut(s)	388, 497
BsaAI	YACGTR	2 cut(s)	55, 610
BsaBI	GATNNNNATC	1 cut(s)	1639
BsaHI	GRCGYC	2 cut(s)	540, 899
BsaI	GGTCTC	1 cut(s)	1545
BsaJI	CCNNGG	5 cut(s)	463, 695, 758, 1224, 1301
BsaXI	ACNNNNNCTCC	6 cut(s)	101, 131, 964, 994, 1499, 1529
Bsc4I	CCNNNNNNNGG	5 cut(s)	87, 346, 759, 830, 1399
Bse1I	ACTGG	1 cut(s)	1160
Bse3DI	GCAATG	1 cut(s)	155
Bse8I	GATNNNNATC	1 cut(s)	1639
BseBI	CCWGG	1 cut(s)	1302
BseDI	CCNNGG	5 cut(s)	463, 695, 758, 1224, 1301
BseGI	GGATG	5 cut(s)	213, 306, 351, 884, 1870
BseJI	GATNNNNATC	1 cut(s)	1639
BseLI	CCNNNNNNNGG	5 cut(s)	87, 346, 759, 830, 1399
BseMI	GCAATG	1 cut(s)	155
BseNI	ACTGG	1 cut(s)	1160
BseRI	GAGGAG	3 cut(s)	938, 985, 1332
BseSI	GKGCMC	1 cut(s)	1589
BseXI	GCAGC	8 cut(s)	47, 462, 518, 555, 1121, 1243, 1246, 1774
BseYI	CCCAGC	1 cut(s)	1774
BsgI	GTGCAG	1 cut(s)	899
Bsh1236I	CGCG	8 cut(s)	140, 285, 366, 465, 470, 581, 760, 1211
Bsh1285I	CGRYCG	2 cut(s)	559, 1215
BshFI	GGCC	4 cut(s)	815, 1007, 1700, 1773
BsiEI	CGRYCG	2 cut(s)	559, 1215
BsiHKAI	GWGCWC	1 cut(s)	1589
BsiHKCI	CYCGRG	1 cut(s)	293
BsiSI	CCGG	4 cut(s)	120, 387, 497, 1004
BslFI	GGGAC	4 cut(s)	29, 137, 403, 878
BslI	CCNNNNNNNGG	5 cut(s)	87, 346, 759, 830, 1399
BsmAI	GTCTC	2 cut(s)	494, 1545
BsmFI	GGGAC	4 cut(s)	29, 137, 403, 878
BsmI	GAATGC	1 cut(s)	634
BsnI	GGCC	4 cut(s)	815, 1007, 1700, 1773
Bso31I	GGTCTC	1 cut(s)	1545
BsoBI	CYCGRG	1 cut(s)	293
Bsp119I	TTCGAA	2 cut(s)	704, 911
Bsp1286I	GDGCHC	2 cut(s)	753, 1589
Bsp143I	GATC	8 cut(s)	63, 303, 623, 987, 1216, 1346, 1634, 1713
BspANI	GGCC	4 cut(s)	815, 1007, 1700, 1773
BspFNI	CGCG	8 cut(s)	140, 285, 366, 465, 470, 581, 760, 1211
BspHI	TCATGA	1 cut(s)	1455
BspLI	GGNNCC	4 cut(s)	79, 816, 1125, 1636
BspMAI	CTGCAG	1 cut(s)	508
BspMI	ACCTGC	1 cut(s)	1223
BspPI	GGATC	4 cut(s)	1211, 1354, 1629, 1642
BspQI	GCTCTTC	2 cut(s)	452, 953
BspT104I	TTCGAA	2 cut(s)	704, 911
BspTNI	GGTCTC	1 cut(s)	1545
BsrBI	CCGCTC	1 cut(s)	963
BsrDI	GCAATG	1 cut(s)	155
BsrI	ACTGG	1 cut(s)	1160
BssECI	CCNNGG	5 cut(s)	463, 695, 758, 1224, 1301
BssMI	GATC	8 cut(s)	63, 303, 623, 987, 1216, 1346, 1634, 1713
BssNAI	GTATAC	1 cut(s)	1274
BssNI	GRCGYC	2 cut(s)	540, 899
BssSI	CACGAG	1 cut(s)	744
BssT1I	CCWWGG	1 cut(s)	1224
Bst1107I	GTATAC	1 cut(s)	1274
Bst2BI	CACGAG	1 cut(s)	744
Bst2UI	CCWGG	1 cut(s)	1302
Bst4CI	ACNGT	4 cut(s)	77, 557, 1382, 1580
Bst6I	CTCTTC	3 cut(s)	263, 452, 953
BstACI	GRCGYC	2 cut(s)	540, 899
BstAPI	GCANNNNNTGC	2 cut(s)	1438, 1784
BstBAI	YACGTR	2 cut(s)	55, 610
BstBI	TTCGAA	2 cut(s)	704, 911
BstC8I	GCNNGC	6 cut(s)	270, 762, 808, 1058, 1328, 1660
BstDEI	CTNAG	3 cut(s)	67, 1178, 1663
BstDSI	CCRYGG	3 cut(s)	463, 695, 758
BstF5I	GGATG	5 cut(s)	213, 306, 351, 884, 1870
BstFNI	CGCG	8 cut(s)	140, 285, 366, 465, 470, 581, 760, 1211
BstH2I	RGCGCY	2 cut(s)	1080, 1115
BstHHI	GCGC	6 cut(s)	285, 368, 470, 1079, 1114, 1211
BstKTI	GATC	8 cut(s)	66, 306, 626, 990, 1219, 1349, 1637, 1716
BstMAI	GTCTC	2 cut(s)	494, 1545
BstMBI	GATC	8 cut(s)	63, 303, 623, 987, 1216, 1346, 1634, 1713
BstMCI	CGRYCG	2 cut(s)	559, 1215
BstMWI	GCNNNNNNNGC	7 cut(s)	129, 146, 757, 812, 1438, 1527, 1784
BstNI	CCWGG	1 cut(s)	1302
BstNSI	RCATGY	1 cut(s)	1832
BstSCI	CCNGG	3 cut(s)	386, 495, 1300
BstSFI	CTRYAG	2 cut(s)	504, 1576
BstSLI	GKGCMC	1 cut(s)	1589
BstUI	CGCG	8 cut(s)	140, 285, 366, 465, 470, 581, 760, 1211
BstV1I	GCAGC	8 cut(s)	47, 462, 518, 555, 1121, 1243, 1246, 1774
BstV2I	GAAGAC	2 cut(s)	1229, 1802
BstX2I	RGATCY	2 cut(s)	987, 1634
BstYI	RGATCY	2 cut(s)	987, 1634
BstZ17I	GTATAC	1 cut(s)	1274
BsuRI	GGCC	4 cut(s)	815, 1007, 1700, 1773
BtgI	CCRYGG	3 cut(s)	463, 695, 758
BtgZI	GCGATG	3 cut(s)	606, 944, 1140
BtrI	CACGTC	1 cut(s)	519
BtsCI	GGATG	5 cut(s)	213, 306, 351, 884, 1870
BtsI	GCAGTG	1 cut(s)	1785
BtsIMutI	CAGTG	3 cut(s)	1197, 1585, 1785
BveI	ACCTGC	1 cut(s)	1223
Cac8I	GCNNGC	6 cut(s)	270, 762, 808, 1058, 1328, 1660
CciI	TCATGA	1 cut(s)	1455
CfoI	GCGC	6 cut(s)	285, 368, 470, 1079, 1114, 1211
Cfr13I	GGNCC	3 cut(s)	814, 1001, 1772
Cfr42I	CCGCGG	2 cut(s)	466, 761
CseI	GACGC	4 cut(s)	400, 587, 621, 907
Csp6I	GTAC	5 cut(s)	433, 460, 1015, 1378, 1558
CspCI	CAANNNNNGTGG	2 cut(s)	1186, 1221
CviQI	GTAC	5 cut(s)	433, 460, 1015, 1378, 1558
DdeI	CTNAG	3 cut(s)	67, 1178, 1663
DpnI	GATC	8 cut(s)	65, 305, 625, 989, 1218, 1348, 1636, 1715
DpnII	GATC	8 cut(s)	63, 303, 623, 987, 1216, 1346, 1634, 1713
DraIII	CACNNNGTG	2 cut(s)	613, 1367
DrdI	GACNNNNNNGTC	1 cut(s)	539
DseDI	GACNNNNNNGTC	1 cut(s)	539
Eam1104I	CTCTTC	3 cut(s)	263, 452, 953
EarI	CTCTTC	3 cut(s)	263, 452, 953
EciI	GGCGGA	1 cut(s)	870
Eco130I	CCWWGG	1 cut(s)	1224
Eco24I	GRGCYC	1 cut(s)	753
Eco31I	GGTCTC	1 cut(s)	1545
Eco47I	GGWCC	1 cut(s)	1001
Eco47III	AGCGCT	1 cut(s)	1078
Eco57I	CTGAAG	1 cut(s)	1082
Eco72I	CACGTG	1 cut(s)	610
Eco88I	CYCGRG	1 cut(s)	293
EcoO109I	RGGNCCY	1 cut(s)	814
EcoRI	GAATTC	1 cut(s)	682
EcoRII	CCWGG	1 cut(s)	1300
EcoT14I	CCWWGG	1 cut(s)	1224
EcoT22I	ATGCAT	2 cut(s)	1156, 1600
EcoT38I	GRGCYC	1 cut(s)	753
ErhI	CCWWGG	1 cut(s)	1224
FalI	AAGNNNNNCTT	2 cut(s)	1161, 1193
FaqI	GGGAC	4 cut(s)	29, 137, 403, 878
FauI	CCCGC	5 cut(s)	753, 765, 956, 1026, 1049
FauNDI	CATATG	1 cut(s)	1131
FbaI	TGATCA	1 cut(s)	303
FblI	GTMKAC	2 cut(s)	1046, 1273
FokI	GGATG	5 cut(s)	200, 313, 338, 871, 1857
FriOI	GRGCYC	1 cut(s)	753
FspBI	CTAG	2 cut(s)	1080, 1461
GlaI	GCGC	6 cut(s)	284, 367, 469, 1078, 1113, 1210
GsaI	CCCAGC	1 cut(s)	1778
GsuI	CTGGAG	3 cut(s)	1428, 1501, 1504
HaeII	RGCGCY	2 cut(s)	1080, 1115
HaeIII	GGCC	4 cut(s)	815, 1007, 1700, 1773
HapII	CCGG	4 cut(s)	120, 387, 497, 1004
HgaI	GACGC	4 cut(s)	400, 587, 621, 907
HhaI	GCGC	6 cut(s)	285, 368, 470, 1079, 1114, 1211
Hin1I	GRCGYC	2 cut(s)	540, 899
Hin6I	GCGC	6 cut(s)	283, 366, 468, 1077, 1112, 1209
HinP1I	GCGC	6 cut(s)	283, 366, 468, 1077, 1112, 1209
HincII	GTYRAC	1 cut(s)	532
HindII	GTYRAC	1 cut(s)	532
HindIII	AAGCTT	1 cut(s)	602
HinfI	GANTC	7 cut(s)	224, 533, 706, 717, 858, 1174, 1297
HpaII	CCGG	4 cut(s)	120, 387, 497, 1004
HphI	GGTGA	4 cut(s)	628, 745, 907, 995
Hpy166II	GTNNAC	5 cut(s)	435, 532, 1047, 1274, 1587
Hpy188I	TCNGA	5 cut(s)	405, 487, 538, 1168, 1654
Hpy188III	TCNNGA	5 cut(s)	560, 721, 746, 1456, 1483
Hpy8I	GTNNAC	5 cut(s)	435, 532, 1047, 1274, 1587
Hpy99I	CGWCG	5 cut(s)	140, 523, 545, 581, 637
HpyAV	CCTTC	9 cut(s)	175, 283, 661, 1163, 1230, 1337, 1393, 1406, 1532
HpyCH4III	ACNGT	4 cut(s)	77, 557, 1382, 1580
HpyCH4IV	ACGT	5 cut(s)	54, 518, 540, 609, 867
HpyF10VI	GCNNNNNNNGC	7 cut(s)	129, 146, 757, 812, 1438, 1527, 1784
HpyF3I	CTNAG	3 cut(s)	67, 1178, 1663
HpySE526I	ACGT	5 cut(s)	54, 518, 540, 609, 867
Hsp92I	GRCGYC	2 cut(s)	540, 899
HspAI	GCGC	6 cut(s)	283, 366, 468, 1077, 1112, 1209
Ksp22I	TGATCA	1 cut(s)	303
KspI	CCGCGG	2 cut(s)	466, 761
Kzo9I	GATC	8 cut(s)	63, 303, 623, 987, 1216, 1346, 1634, 1713
LguI	GCTCTTC	2 cut(s)	452, 953
LmnI	GCTCC	3 cut(s)	122, 972, 1123
Lsp1109I	GCAGC	8 cut(s)	47, 462, 518, 555, 1121, 1243, 1246, 1774
LweI	GCATC	4 cut(s)	360, 1163, 1271, 1645
MaeI	CTAG	2 cut(s)	1080, 1461
MaeII	ACGT	5 cut(s)	54, 518, 540, 609, 867
MaeIII	GTNAC	2 cut(s)	660, 895
MalI	GATC	8 cut(s)	65, 305, 625, 989, 1218, 1348, 1636, 1715
MbiI	CCGCTC	1 cut(s)	963
MboI	GATC	8 cut(s)	63, 303, 623, 987, 1216, 1346, 1634, 1713
MfeI	CAATTG	1 cut(s)	1623
MflI	RGATCY	2 cut(s)	987, 1634
MhlI	GDGCHC	2 cut(s)	753, 1589
MluCI	AATT	7 cut(s)	209, 682, 913, 1280, 1284, 1355, 1623
MlyI	GAGTC	4 cut(s)	218, 527, 726, 1306
MmeI	TCCRAC	1 cut(s)	207
Mph1103I	ATGCAT	2 cut(s)	1156, 1600
MroXI	GAANNNNTTC	1 cut(s)	98
MseI	TTAA	4 cut(s)	801, 1283, 1766, 1897
MslI	CAYNNNNRTG	3 cut(s)	440, 1245, 1251
MspA1I	CMGCKG	3 cut(s)	465, 568, 760
MspI	CCGG	4 cut(s)	120, 387, 497, 1004
MspR9I	CCNGG	3 cut(s)	388, 497, 1302
MunI	CAATTG	1 cut(s)	1623
Mva1269I	GAATGC	1 cut(s)	634
MvaI	CCWGG	1 cut(s)	1302
MvnI	CGCG	8 cut(s)	140, 285, 366, 465, 470, 581, 760, 1211
MwoI	GCNNNNNNNGC	7 cut(s)	129, 146, 757, 812, 1438, 1527, 1784
NciI	CCSGG	2 cut(s)	388, 497
NdeI	CATATG	1 cut(s)	1131
NdeII	GATC	8 cut(s)	63, 303, 623, 987, 1216, 1346, 1634, 1713
NlaIV	GGNNCC	4 cut(s)	79, 816, 1125, 1636
NmeAIII	GCCGAG	1 cut(s)	927
NmuCI	GTSAC	2 cut(s)	660, 895
NsiI	ATGCAT	2 cut(s)	1156, 1600
NspI	RCATGY	1 cut(s)	1832
NspV	TTCGAA	2 cut(s)	704, 911
PaeR7I	CTCGAG	1 cut(s)	293
PagI	TCATGA	1 cut(s)	1455
PciSI	GCTCTTC	2 cut(s)	452, 953
PcsI	WCGNNNNNNNCGW	2 cut(s)	246, 632
PctI	GAATGC	1 cut(s)	634
PdmI	GAANNNNTTC	1 cut(s)	98
PfeI	GAWTC	3 cut(s)	706, 858, 1174
PfoI	TCCNGGA	2 cut(s)	386, 495
PleI	GAGTC	4 cut(s)	218, 527, 725, 1305
PmaCI	CACGTG	1 cut(s)	610
PmlI	CACGTG	1 cut(s)	610
PpsI	GAGTC	4 cut(s)	218, 527, 725, 1305
Ppu21I	YACGTR	2 cut(s)	55, 610
PshBI	ATTAAT	1 cut(s)	1283
PsiI	TTATAA	1 cut(s)	954
Psp6I	CCWGG	1 cut(s)	1300
PspCI	CACGTG	1 cut(s)	610
PspFI	CCCAGC	1 cut(s)	1774
PspGI	CCWGG	1 cut(s)	1300
PspN4I	GGNNCC	4 cut(s)	79, 816, 1125, 1636
PspPI	GGNCC	3 cut(s)	814, 1001, 1772
PspXI	VCTCGAGB	1 cut(s)	293
PsrI	GAACNNNNNNTAC	2 cut(s)	1824, 1856
PstI	CTGCAG	1 cut(s)	508
PsuI	RGATCY	2 cut(s)	987, 1634
RsaI	GTAC	5 cut(s)	434, 461, 1016, 1379, 1559
RsaNI	GTAC	5 cut(s)	433, 460, 1015, 1378, 1558
RseI	CAYNNNNRTG	3 cut(s)	440, 1245, 1251
SacII	CCGCGG	2 cut(s)	466, 761
SapI	GCTCTTC	2 cut(s)	452, 953
SaqAI	TTAA	4 cut(s)	801, 1283, 1766, 1897
Sau3AI	GATC	8 cut(s)	63, 303, 623, 987, 1216, 1346, 1634, 1713
Sau96I	GGNCC	3 cut(s)	814, 1001, 1772
SchI	GAGTC	4 cut(s)	218, 527, 726, 1306
ScrFI	CCNGG	3 cut(s)	388, 497, 1302
SduI	GDGCHC	2 cut(s)	753, 1589
SfaNI	GCATC	4 cut(s)	360, 1163, 1271, 1645
SfcI	CTRYAG	2 cut(s)	504, 1576
Sfr274I	CTCGAG	1 cut(s)	293
Sfr303I	CCGCGG	2 cut(s)	466, 761
SfuI	TTCGAA	2 cut(s)	704, 911
SgrBI	CCGCGG	2 cut(s)	466, 761
SinI	GGWCC	1 cut(s)	1001
SlaI	CTCGAG	1 cut(s)	293
SmiMI	CAYNNNNRTG	3 cut(s)	440, 1245, 1251
SmlI	CTYRAG	2 cut(s)	293, 1331
SmoI	CTYRAG	2 cut(s)	293, 1331
Sse9I	AATT	7 cut(s)	209, 682, 913, 1280, 1284, 1355, 1623
SspI	AATATT	1 cut(s)	1647
SspMI	CTAG	2 cut(s)	1080, 1461
StyD4I	CCNGG	3 cut(s)	386, 495, 1300
StyI	CCWWGG	1 cut(s)	1224
TaaI	ACNGT	4 cut(s)	77, 557, 1382, 1580
TaiI	ACGT	5 cut(s)	57, 521, 543, 612, 870
TaqII	GACCGA	2 cut(s)	1229, 1796
TasI	AATT	7 cut(s)	209, 682, 913, 1280, 1284, 1355, 1623
TatI	WGTACW	1 cut(s)	1557
TauI	GCSGC	3 cut(s)	288, 468, 568
TfiI	GAWTC	3 cut(s)	706, 858, 1174
Tru1I	TTAA	4 cut(s)	801, 1283, 1766, 1897
Tru9I	TTAA	4 cut(s)	801, 1283, 1766, 1897
TscAI	CASTG	3 cut(s)	1204, 1585, 1785
TseFI	GTSAC	2 cut(s)	660, 895
TseI	GCWGC	8 cut(s)	35, 475, 506, 568, 1109, 1256, 1259, 1787
Tsp45I	GTSAC	2 cut(s)	660, 895
TspDTI	ATGAA	8 cut(s)	163, 202, 215, 439, 947, 953, 1229, 1684
TspGWI	ACGGA	4 cut(s)	70, 797, 846, 1002
TspRI	CASTG	3 cut(s)	1204, 1585, 1785
VneI	GTGCAC	1 cut(s)	1585
VpaK11BI	GGWCC	1 cut(s)	1001
VspI	ATTAAT	1 cut(s)	1283
XapI	RAATTY	3 cut(s)	682, 913, 1355
XceI	RCATGY	1 cut(s)	1832
XhoI	CTCGAG	1 cut(s)	293
XmiI	GTMKAC	2 cut(s)	1046, 1273
XmnI	GAANNNNTTC	1 cut(s)	98
XspI	CTAG	2 cut(s)	1080, 1461
ZraI	GACGTC	1 cut(s)	541
Zsp2I	ATGCAT	2 cut(s)	1156, 1600

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.