pycom17g02730

zinc finger CCCH domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0011914
Physical Location & Seq
Reverse (-)
1820557 .. 1825604
5048 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2826 bp
ATGAGCCAACGTTCTAGTGATTTCGAGGGATCAATGGAGTTAAAAGTTTCATCTCCAAAACCTGGGGGTCTTTCCCCTTCTGATTGCAGTAGTGATCCTGAGGAGAAGGAAGTAAGTGATGATGATGATGACGATCGAAACCACAAGCATCGTAGACGAGATACTCGCTCTCAGTCTTCGGAGAGAGATACTTTTGATCAAGTCACAACAAGGCCATTCAGAAAGCGAAACAAACCTTTTGTAAATGGGAACTCTTTTAGAGATAATGACTCTCAAGCAAGTACACCATGGAGAAATCACAATTCCACTGACTTTTCTGTAAAATTTGATAAAAGACGCCCGGGGTCAGCATCACTGCCTCGAGCACCTTTTGATTTAAATCAAAGAATCCGGGCAAACCAAGCATTTCCTGGAGACCTTGGTCCTGGTAGGGGAAGAGGACGGGACTTTGGATCATGGAGCCAACGCGGTCCTAGGTTCAACTCAGATCTTGCTTCTCAAATGGTTCATCAGGGCTCCATCCGTCCGAGTCTTTTTGCTGGGCGTGGATTGCCAAATGTTTCCGGTGCACAAAGTGGATCCTGGAATGCATTTGGATTGATTCCAGGCATACCAAATGGTTCCATGGACACACTACATTCCATTGGCTTGCAAGGAACACTCAGACCACCAATCCATTCTTCATTAAATATGGGGATTCCTCGCCAAAGATGTAGAGACTTCGAGGAGCGTGGATTTTGTCTCAGAGGGGATGTGTGCCCAATGGAGCATGGTATGAATCGGATTGTTATTGAAGATGTCCAGAGTCTTTCCCAGTTCAACCTCCCTGTGTCGATTCCAAGTGCACACTTACTGGGAAAACCCACAGGCCCGGGATCTCTACCATCAGTGAGTGCATCCTCAACTGCATTGATGAATAGCAAAGGCTTAAATGTAAAAACAAGCAAGACTGCTGTCACTGATGATGGCTTGGGCTTGAGTGGTTCATATTCTGGTACTGGTTATGCAGGTGGTGCTGATTTGTATGACCCTGATCAACCCCTGTGGAATAATAACTGTCCTGAAACCTCAAATGCACTTCTAGGCCTGCGATCACCCAGGAATGATGAAACTGAAACCTTGTCGAATGATGCTGATAATGAATTCCCAATTAGAAGCGTTGGGGCAGCTGCCAGTTCACAGAGTACAAGCATGTCTGTGTGGGGTAGAATTGGTAGTTCAAAAAGTAGATTAGATGTGAAGGAGAAAGTCGATCCTGTAATAAATTCTTCAGATTATATTGAGAGTGATACCAAGGAAGCGAAGGAGGCAGTCAACACTCAAACTCCACCACATCAAGGAAAGCGTATAATTGCAGAAGATTGCCCAAACACCATGGATTCATCACCCCAGACACCTTTTGATTCCACGCGTAACATTCGGAGATCATCTCAAAAAGCACTCCGTACTCTATTTGTTAATGGCATTCCTCAGAAAAACAACAAAAGGGAGTCTCTTCTTTCTCATTTTCAAAAATTCGGAGAGGTCATTGACATCTACATTCCATCAAATAGTGAACGAGCTTTTGTCCAGTTTTCAAGGAGGGAAGAGGCTGAAGCTGCTTTAAAGGCACCTGATGCTGTAATGGGTAACCGATTTATCAGGTTGTGGTGGGCTAATCGTGATAGCATTCCTGATGAGGGGACAGGCACTCCCAGTATTTTTTCCGGAACTCCCCATGGTGTAACAACTGGTTCAGTGCCACCCCATTCATCTGGTGCTAGTATTAGCAAGGATAATCTTCAGTCTGCAGCTCTAAAGGGTAGTATTGCCCATGCATCTGATGCTTCTCTACCTGCCACTGACAACTCCAAGCCTATCACCTCAAATGGTCCCAAGGCCCCACCTCTGCAAAAAAAGCTAGAGAGTTTGGAGCAGTTGAAGGAGGAACTTCGCAAGAAGCAGGAAATGCTAGCCCAGAAAAGGAATGACTTTCGTCGCCAGTTGGACAAGCTTGAGAAACAAGCTACTGGACCCAAGAGTGAGACTGACACAGAGCAAGCTGCTAAGAGACCCAAAGTGGGAACAACAACCGAAGCTGATAAAGCTGCTACTCCAAGCTCCTCAAATCCTGCTCCTGCGGATGCAATGCATGCTGAGATGACTGATAAGAACAAATCAGAGGAGAACATTGTGTCCGGTAGTCCAAAAACCAGTACACCTGTGATGCTGCAGCAATCTCCTAGCTTGAAGCTGCAGACTATCCGTCCATTGGGACCACTCGGGCCACCTTCTCCTTTCAATAGATATAAATTAGACAACCGTCCTACTGGTTTTATAATTCTTCCTCCTTTACCAGTTGGATTTGCAAATGTTGCTATTATGAAGGAACACTTTTCACCCTATGGCGATCTTTCTACTGTGGAATTAGAAGATCTGGAATCCCGTGATTGTGGAAGTGAGTTAGAGGAATCAAAAGATTGCTCAGCTCGTATAACTTTCACTACACGCCGTTCAGCAGAGAGGGCATTTCTCAACGGTAAATGCTGGGAAGGGCACGATCTGAAATTTATGTGGCTGACATCTAGTATTTCCAGCAATGACCATAGTGGCAGAGAAAACTCTCCATCTACCACTCCTAAGGGGCCATTACTAGCTGATGCTCAGCCTGTTGCAGATAAAGAAGCATACATTGTTTCCCAGGAAGCTGCTGCATCAGGAAATGGAGAACCGGAACATTCTGAACGAATAAGTGGTGTTGAGCAGGTGGAATCGGATGAACGTGCCGAGTCTAGTCCAAGGGCAACATCAGGCGAGAAAGAGTCATCTCGGGGCGATGCATTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005488 GO:0097159 GO:1901363
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RRM_1

Protein Analysis

942

Amino Acids

102.35

Weight (kDa)

6.46

Isoelectric Point (pI)

53.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 484 - 547 1.8e-07 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011914)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G27700 AT3G27700
fragaria_vesca FvH4_6g51350
malus_domestica MD09G1028400.v1.1 MD17G1029900.v1.1
prunus_persica Prupe.3G289600_v2.0.a1 Prupe.3G290100_v2.0.a1
pyrus_communis pycom111g02240 pycom17g02730
rosa_chinensis RchiOBHm_Chr2g0173051
rosa_laevigata RLG00000022152
rosa_multiflora Rmu_sc0011236.1_g000003
rosa_roxburghii Rroxscaffold_2G00079290
rosa_rugosa Rorug02G0564800
rosa_samantha Rh2AG644300 Rh2BG655100 Rh2CG620400 Rh2DG668600
rosa_wichuraiana Rw2G052940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2318
AarI CACCTGC 2 cut(s) 998, 2734
Acc36I ACCTGC 3 cut(s) 998, 1843, 2734
AccB1I GGYRCC 1 cut(s) 1609
AccB7I CCANNNNNTGG 1 cut(s) 62
AccI GTMKAC 1 cut(s) 154
AccII CGCG 2 cut(s) 468, 1411
AccIII TCCGGA 1 cut(s) 1706
AciI CCGC 2 cut(s) 468, 2120
AclI AACGTT 1 cut(s) 10
AclWI GGATC 7 cut(s) 37, 89, 460, 573, 586, 883, 1247
AcsI RAATTY 5 cut(s) 323, 1142, 1264, 1514, 2546
AcuI CTGAAG 3 cut(s) 1254, 1614, 1766
AcyI GRCGYC 1 cut(s) 337
AdeI CACNNNGTG 1 cut(s) 575
AfaI GTAC 5 cut(s) 283, 997, 1186, 1447, 2197
AfiI CCNNNNNNNGG 7 cut(s) 62, 431, 1337, 1679, 1962, 2060, 2251
AflIII ACRYGT 1 cut(s) 1409
AgsI TTSAA 9 cut(s) 481, 794, 820, 1221, 1511, 1578, 1921, 2230, 2281
AhdI GACNNNNNGTC 1 cut(s) 2244
AjnI CCWGG 7 cut(s) 61, 409, 424, 581, 604, 1097, 2679
AjuI GAANNNNNNNTTGG 2 cut(s) 664, 696
AloI GAACNNNNNNTCC 2 cut(s) 2159, 2191
Alw21I GWGCWC 3 cut(s) 367, 571, 847
Alw26I GTCTC 6 cut(s) 408, 711, 746, 1497, 2018, 2044
Alw44I GTGCAC 2 cut(s) 567, 843
AlwI GGATC 7 cut(s) 37, 89, 460, 573, 586, 883, 1247
Ama87I CYCGRG 5 cut(s) 340, 360, 871, 2261, 2808
Aor13HI TCCGGA 1 cut(s) 1706
AoxI GGCC 6 cut(s) 212, 868, 1084, 1878, 2264, 2624
ApaLI GTGCAC 2 cut(s) 567, 843
ApoI RAATTY 5 cut(s) 323, 1142, 1264, 1514, 2546
AspA2I CCTAGG 1 cut(s) 473
AspS9I GGNCC 9 cut(s) 422, 470, 869, 1871, 1879, 2012, 2255, 2264, 2624
AsuC2I CCSGG 5 cut(s) 341, 342, 392, 872, 873
AsuHPI GGTGA 4 cut(s) 1086, 1377, 1852, 2370
AsuNHI GCTAGC 1 cut(s) 1951
AvaI CYCGRG 5 cut(s) 340, 360, 871, 2261, 2808
AvaII GGWCC 5 cut(s) 422, 470, 1871, 2012, 2255
AvrII CCTAGG 1 cut(s) 473
AxyI CCTNAGG 2 cut(s) 99, 2619
BaeGI GKGCMC 4 cut(s) 571, 761, 847, 2538
BamHI GGATCC 1 cut(s) 578
BanI GGYRCC 1 cut(s) 1609
BanII GRGCYC 1 cut(s) 518
BbsI GAAGAC 1 cut(s) 168
Bbv12I GWGCWC 3 cut(s) 367, 571, 847
BccI CCATC 5 cut(s) 527, 892, 959, 1552, 2614
BceAI ACGGC 1 cut(s) 2475
BciT130I CCWGG 7 cut(s) 63, 411, 426, 583, 606, 1099, 2681
BclI TGATCA 2 cut(s) 196, 1033
BcnI CCSGG 5 cut(s) 341, 342, 392, 872, 873
BcoDI GTCTC 6 cut(s) 408, 711, 746, 1497, 2018, 2044
BfmI CTRYAG 3 cut(s) 1788, 2210, 2234
BfuAI ACCTGC 3 cut(s) 998, 1843, 2734
BglII AGATCT 2 cut(s) 487, 2413
BlnI CCTAGG 1 cut(s) 473
BlpI GCTNAGC 2 cut(s) 2464, 2643
Bme18I GGWCC 5 cut(s) 422, 470, 1871, 2012, 2255
BmeRI GACNNNNNGTC 1 cut(s) 2244
BmeT110I CYCGRG 5 cut(s) 340, 360, 871, 2261, 2808
BmgT120I GGNCC 9 cut(s) 422, 470, 869, 1871, 1879, 2012, 2255, 2264, 2624
BmrI ACTGGG 3 cut(s) 808, 863, 1689
BmtI GCTAGC 1 cut(s) 1955
BmuI ACTGGG 3 cut(s) 808, 863, 1689
BoxI GACNNNNGTC 4 cut(s) 420, 953, 1974, 2301
BpiI GAAGAC 1 cut(s) 168
BplI GAGNNNNNCTC 2 cut(s) 1414, 1446
BpmI CTGGAG 1 cut(s) 432
Bpu1102I GCTNAGC 2 cut(s) 2464, 2643
BpuEI CTTGAG 3 cut(s) 258, 997, 2015
BpuMI CCSGG 5 cut(s) 341, 342, 392, 872, 873
BsaBI GATNNNNATC 2 cut(s) 132, 378
BsaHI GRCGYC 1 cut(s) 337
BsaI GGTCTC 2 cut(s) 408, 2044
BsaWI WCCGGW 4 cut(s) 563, 1706, 2177, 2710
BsaXI ACNNNNNCTCC 4 cut(s) 1309, 1339, 1675, 1705
Bsc4I CCNNNNNNNGG 7 cut(s) 62, 431, 1337, 1679, 1962, 2060, 2251
Bse21I CCTNAGG 2 cut(s) 99, 2619
Bse3DI GCAATG 2 cut(s) 2133, 2584
Bse8I GATNNNNATC 2 cut(s) 132, 378
BseAI TCCGGA 1 cut(s) 1706
BseBI CCWGG 7 cut(s) 63, 411, 426, 583, 606, 1099, 2681
BseGI GGATG 5 cut(s) 519, 757, 896, 2128, 2761
BseJI GATNNNNATC 2 cut(s) 132, 378
BseLI CCNNNNNNNGG 7 cut(s) 62, 431, 1337, 1679, 1962, 2060, 2251
BseMI GCAATG 2 cut(s) 2133, 2584
BseMII CTCAG 9 cut(s) 90, 185, 498, 676, 757, 1484, 2127, 2478, 2657
BseRI GAGGAG 4 cut(s) 116, 740, 2092, 2177
BseSI GKGCMC 4 cut(s) 571, 761, 847, 2538
BseYI CCCAGC 2 cut(s) 539, 2526
Bsh1236I CGCG 2 cut(s) 468, 1411
Bsh1285I CGRYCG 1 cut(s) 136
BshFI GGCC 6 cut(s) 214, 870, 1086, 1880, 2266, 2626
BshNI GGYRCC 1 cut(s) 1609
BsiEI CGRYCG 1 cut(s) 136
BsiHKAI GWGCWC 3 cut(s) 367, 571, 847
BsiHKCI CYCGRG 5 cut(s) 340, 360, 871, 2261, 2808
BsiSI CCGG 7 cut(s) 341, 391, 564, 872, 1707, 2178, 2711
BslFI GGGAC 4 cut(s) 458, 1696, 1857, 2268
BslI CCNNNNNNNGG 7 cut(s) 62, 431, 1337, 1679, 1962, 2060, 2251
BsmAI GTCTC 6 cut(s) 408, 711, 746, 1497, 2018, 2044
BsmFI GGGAC 4 cut(s) 458, 1696, 1857, 2268
BsmI GAATGC 3 cut(s) 592, 1464, 1668
BsnI GGCC 6 cut(s) 214, 870, 1086, 1880, 2266, 2626
Bso31I GGTCTC 2 cut(s) 408, 2044
BsoBI CYCGRG 5 cut(s) 340, 360, 871, 2261, 2808
Bsp1286I GDGCHC 6 cut(s) 367, 518, 571, 761, 847, 2538
Bsp13I TCCGGA 1 cut(s) 1706
Bsp1720I GCTNAGC 2 cut(s) 2464, 2643
Bsp19I CCATGG 4 cut(s) 287, 624, 1374, 1717
BspACI CCGC 2 cut(s) 468, 2120
BspANI GGCC 6 cut(s) 214, 870, 1086, 1880, 2266, 2626
BspCNI CTCAG 9 cut(s) 91, 184, 497, 675, 756, 1483, 2128, 2477, 2656
BspEI TCCGGA 1 cut(s) 1706
BspFNI CGCG 2 cut(s) 468, 1411
BspMAI CTGCAG 3 cut(s) 1792, 2214, 2238
BspMI ACCTGC 3 cut(s) 998, 1843, 2734
BspOI GCTAGC 1 cut(s) 1955
BspPI GGATC 7 cut(s) 37, 89, 460, 573, 586, 883, 1247
BspT107I GGYRCC 1 cut(s) 1609
BspTNI GGTCTC 2 cut(s) 408, 2044
BsrDI GCAATG 2 cut(s) 2133, 2584
BssNI GRCGYC 1 cut(s) 337
BssT1I CCWWGG 9 cut(s) 287, 418, 473, 624, 1293, 1374, 1717, 1875, 2777
Bst2UI CCWGG 7 cut(s) 63, 411, 426, 583, 606, 1099, 2681
Bst4CI ACNGT 4 cut(s) 1058, 2303, 2401, 2519
Bst6I CTCTTC 3 cut(s) 430, 1500, 1581
BstACI GRCGYC 1 cut(s) 337
BstAPI GCANNNNNTGC 6 cut(s) 1013, 1616, 1823, 1948, 2132, 2354
BstC8I GCNNGC 5 cut(s) 650, 1088, 1953, 2040, 2133
BstDSI CCRYGG 4 cut(s) 287, 624, 1374, 1717
BstEII GGTNACC 1 cut(s) 1628
BstF5I GGATG 5 cut(s) 519, 757, 896, 2128, 2761
BstFNI CGCG 2 cut(s) 468, 1411
BstMAI GTCTC 6 cut(s) 408, 711, 746, 1497, 2018, 2044
BstMCI CGRYCG 1 cut(s) 136
BstNI CCWGG 7 cut(s) 63, 411, 426, 583, 606, 1099, 2681
BstNSI RCATGY 2 cut(s) 1195, 2135
BstPAI GACNNNNGTC 4 cut(s) 420, 953, 1974, 2301
BstPI GGTNACC 1 cut(s) 1628
BstSFI CTRYAG 3 cut(s) 1788, 2210, 2234
BstSLI GKGCMC 4 cut(s) 571, 761, 847, 2538
BstUI CGCG 2 cut(s) 468, 1411
BstV2I GAAGAC 1 cut(s) 168
BstX2I RGATCY 4 cut(s) 487, 578, 875, 2413
BstXI CCANNNNNNTGG 1 cut(s) 1754
BstYI RGATCY 4 cut(s) 487, 578, 875, 2413
Bsu36I CCTNAGG 2 cut(s) 99, 2619
BsuRI GGCC 6 cut(s) 214, 870, 1086, 1880, 2266, 2626
BtgI CCRYGG 4 cut(s) 287, 624, 1374, 1717
BtsCI GGATG 5 cut(s) 519, 757, 896, 2128, 2761
BtsI GCAGTG 1 cut(s) 353
BtsIMutI CAGTG 6 cut(s) 306, 353, 894, 957, 1743, 1839
BveI ACCTGC 3 cut(s) 998, 1843, 2734
Cac8I GCNNGC 5 cut(s) 650, 1088, 1953, 2040, 2133
Cfr13I GGNCC 9 cut(s) 422, 470, 869, 1871, 1879, 2012, 2255, 2264, 2624
Cfr9I CCCGGG 2 cut(s) 340, 871
CseI GACGC 1 cut(s) 345
Csp6I GTAC 5 cut(s) 282, 996, 1185, 1446, 2196
CspCI CAANNNNNGTGG 2 cut(s) 1025, 1060
CviAII CATG 9 cut(s) 288, 456, 625, 770, 1192, 1375, 1718, 1814, 2132
CviQI GTAC 5 cut(s) 282, 996, 1185, 1446, 2196
DraI TTTAAA 2 cut(s) 378, 1605
DraIII CACNNNGTG 1 cut(s) 575
DriI GACNNNNNGTC 1 cut(s) 2244
Eam1104I CTCTTC 3 cut(s) 430, 1500, 1581
Eam1105I GACNNNNNGTC 1 cut(s) 2244
EarI CTCTTC 3 cut(s) 430, 1500, 1581
Eco130I CCWWGG 9 cut(s) 287, 418, 473, 624, 1293, 1374, 1717, 1875, 2777
Eco147I AGGCCT 1 cut(s) 1086
Eco24I GRGCYC 1 cut(s) 518
Eco31I GGTCTC 2 cut(s) 408, 2044
Eco47I GGWCC 5 cut(s) 422, 470, 1871, 2012, 2255
Eco57I CTGAAG 3 cut(s) 1254, 1614, 1766
Eco81I CCTNAGG 2 cut(s) 99, 2619
Eco88I CYCGRG 5 cut(s) 340, 360, 871, 2261, 2808
Eco91I GGTNACC 1 cut(s) 1628
EcoO109I RGGNCCY 1 cut(s) 1879
EcoO65I GGTNACC 1 cut(s) 1628
EcoRI GAATTC 1 cut(s) 1142
EcoRII CCWGG 7 cut(s) 61, 409, 424, 581, 604, 1097, 2679
EcoT14I CCWWGG 9 cut(s) 287, 418, 473, 624, 1293, 1374, 1717, 1875, 2777
EcoT22I ATGCAT 4 cut(s) 592, 1819, 2133, 2821
EcoT38I GRGCYC 1 cut(s) 518
ErhI CCWWGG 9 cut(s) 287, 418, 473, 624, 1293, 1374, 1717, 1875, 2777
FaeI CATG 9 cut(s) 291, 459, 628, 773, 1195, 1378, 1721, 1817, 2135
FalI AAGNNNNNCTT 2 cut(s) 2357, 2389
FaqI GGGAC 4 cut(s) 458, 1696, 1857, 2268
FatI CATG 9 cut(s) 287, 455, 624, 769, 1191, 1374, 1717, 1813, 2131
FbaI TGATCA 2 cut(s) 196, 1033
FblI GTMKAC 1 cut(s) 154
FokI GGATG 5 cut(s) 506, 764, 883, 2135, 2768
FriOI GRGCYC 1 cut(s) 518
GsaI CCCAGC 2 cut(s) 543, 2530
GsuI CTGGAG 1 cut(s) 432
HaeIII GGCC 6 cut(s) 214, 870, 1086, 1880, 2266, 2626
HapII CCGG 7 cut(s) 341, 391, 564, 872, 1707, 2178, 2711
HgaI GACGC 1 cut(s) 345
Hin1I GRCGYC 1 cut(s) 337
Hin1II CATG 9 cut(s) 291, 459, 628, 773, 1195, 1378, 1721, 1817, 2135
HincII GTYRAC 1 cut(s) 1315
HindII GTYRAC 1 cut(s) 1315
HindIII AAGCTT 1 cut(s) 1991
HpaII CCGG 7 cut(s) 341, 391, 564, 872, 1707, 2178, 2711
HphI GGTGA 4 cut(s) 1086, 1377, 1852, 2370
Hpy166II GTNNAC 8 cut(s) 155, 284, 569, 845, 1178, 1315, 1556, 2198
Hpy188III TCNNGA 8 cut(s) 98, 802, 1061, 1661, 1673, 1707, 2417, 2697
Hpy8I GTNNAC 8 cut(s) 155, 284, 569, 845, 1178, 1315, 1556, 2198
Hpy99I CGWCG 1 cut(s) 1980
HpyAV CCTTC 8 cut(s) 87, 100, 1234, 1297, 1915, 2280, 2359, 2525
HpyCH4III ACNGT 4 cut(s) 1058, 2303, 2401, 2519
HpyCH4IV ACGT 2 cut(s) 10, 2761
HpySE526I ACGT 2 cut(s) 10, 2761
Hsp92I GRCGYC 1 cut(s) 337
Hsp92II CATG 9 cut(s) 291, 459, 628, 773, 1195, 1378, 1721, 1817, 2135
Kpn2I TCCGGA 1 cut(s) 1706
Ksp22I TGATCA 2 cut(s) 196, 1033
LmnI GCTCC 7 cut(s) 459, 521, 727, 766, 1912, 2105, 2119
MaeII ACGT 2 cut(s) 10, 2761
MaeIII GTNAC 5 cut(s) 202, 955, 1412, 1628, 1723
MflI RGATCY 4 cut(s) 487, 578, 875, 2413
MhlI GDGCHC 6 cut(s) 367, 518, 571, 761, 847, 2538
MluI ACGCGT 1 cut(s) 1409
MlyI GAGTC 6 cut(s) 263, 538, 814, 1499, 2777, 2810
MmeI TCCRAC 2 cut(s) 1965, 2320
Mph1103I ATGCAT 4 cut(s) 592, 1819, 2133, 2821
MroI TCCGGA 1 cut(s) 1706
MseI TTAA 6 cut(s) 41, 377, 686, 929, 1458, 1604
MslI CAYNNNNRTG 1 cut(s) 1196
MspA1I CMGCKG 1 cut(s) 1169
MspI CCGG 7 cut(s) 341, 391, 564, 872, 1707, 2178, 2711
Mva1269I GAATGC 3 cut(s) 592, 1464, 1668
MvaI CCWGG 7 cut(s) 63, 411, 426, 583, 606, 1099, 2681
MvnI CGCG 2 cut(s) 468, 1411
NciI CCSGG 5 cut(s) 341, 342, 392, 872, 873
NcoI CCATGG 4 cut(s) 287, 624, 1374, 1717
NheI GCTAGC 1 cut(s) 1951
NlaIII CATG 9 cut(s) 291, 459, 628, 773, 1195, 1378, 1721, 1817, 2135
NmeAIII GCCGAG 1 cut(s) 2791
NmuCI GTSAC 2 cut(s) 202, 955
NsiI ATGCAT 4 cut(s) 592, 1819, 2133, 2821
NspI RCATGY 2 cut(s) 1195, 2135
PaeI GCATGC 1 cut(s) 2135
PaeR7I CTCGAG 1 cut(s) 360
PaqCI CACCTGC 2 cut(s) 998, 2734
PceI AGGCCT 1 cut(s) 1086
PctI GAATGC 3 cut(s) 592, 1464, 1668
PflMI CCANNNNNTGG 1 cut(s) 62
PfoI TCCNGGA 2 cut(s) 409, 581
Ple19I CGATCG 1 cut(s) 136
PleI GAGTC 6 cut(s) 263, 537, 813, 1498, 2776, 2809
PpsI GAGTC 6 cut(s) 263, 537, 813, 1498, 2776, 2809
PshAI GACNNNNGTC 4 cut(s) 420, 953, 1974, 2301
PsiI TTATAA 1 cut(s) 2318
Psp1406I AACGTT 1 cut(s) 10
Psp6I CCWGG 7 cut(s) 61, 409, 424, 581, 604, 1097, 2679
PspEI GGTNACC 1 cut(s) 1628
PspFI CCCAGC 2 cut(s) 539, 2526
PspGI CCWGG 7 cut(s) 61, 409, 424, 581, 604, 1097, 2679
PspPI GGNCC 9 cut(s) 422, 470, 869, 1871, 1879, 2012, 2255, 2264, 2624
PspXI VCTCGAGB 1 cut(s) 360
PstI CTGCAG 3 cut(s) 1792, 2214, 2238
PsuI RGATCY 4 cut(s) 487, 578, 875, 2413
PvuI CGATCG 1 cut(s) 136
PvuII CAGCTG 1 cut(s) 1169
RsaI GTAC 5 cut(s) 283, 997, 1186, 1447, 2197
RsaNI GTAC 5 cut(s) 282, 996, 1185, 1446, 2196
RseI CAYNNNNRTG 1 cut(s) 1196
SaqAI TTAA 6 cut(s) 41, 377, 686, 929, 1458, 1604
Sau96I GGNCC 9 cut(s) 422, 470, 869, 1871, 1879, 2012, 2255, 2264, 2624
SchI GAGTC 6 cut(s) 263, 538, 814, 1499, 2777, 2810
SduI GDGCHC 6 cut(s) 367, 518, 571, 761, 847, 2538
SfcI CTRYAG 3 cut(s) 1788, 2210, 2234
Sfr274I CTCGAG 1 cut(s) 360
SinI GGWCC 5 cut(s) 422, 470, 1871, 2012, 2255
SlaI CTCGAG 1 cut(s) 360
SmaI CCCGGG 2 cut(s) 342, 873
SmiI ATTTAAAT 1 cut(s) 378
SmiMI CAYNNNNRTG 1 cut(s) 1196
SmlI CTYRAG 4 cut(s) 273, 360, 976, 1994
SmoI CTYRAG 4 cut(s) 273, 360, 976, 1994
SphI GCATGC 1 cut(s) 2135
SseBI AGGCCT 1 cut(s) 1086
SsiI CCGC 2 cut(s) 468, 2120
StuI AGGCCT 1 cut(s) 1086
StyI CCWWGG 9 cut(s) 287, 418, 473, 624, 1293, 1374, 1717, 1875, 2777
SwaI ATTTAAAT 1 cut(s) 378
TaaI ACNGT 4 cut(s) 1058, 2303, 2401, 2519
TaiI ACGT 2 cut(s) 13, 2764
TaqI TCGA 7 cut(s) 24, 136, 361, 723, 833, 1124, 1251
TatI WGTACW 3 cut(s) 281, 1184, 2195
Tru1I TTAA 6 cut(s) 41, 377, 686, 929, 1458, 1604
Tru9I TTAA 6 cut(s) 41, 377, 686, 929, 1458, 1604
TscAI CASTG 6 cut(s) 313, 360, 894, 964, 1743, 1846
TseFI GTSAC 2 cut(s) 202, 955
Tsp45I GTSAC 2 cut(s) 202, 955
TspGWI ACGGA 3 cut(s) 512, 1433, 2234
TspMI CCCGGG 2 cut(s) 340, 871
TspRI CASTG 6 cut(s) 313, 360, 894, 964, 1743, 1846
Van91I CCANNNNNTGG 1 cut(s) 62
VneI GTGCAC 2 cut(s) 567, 843
VpaK11BI GGWCC 5 cut(s) 422, 470, 1871, 2012, 2255
XapI RAATTY 5 cut(s) 323, 1142, 1264, 1514, 2546
XceI RCATGY 2 cut(s) 1195, 2135
XcmI CCANNNNNNNNNTGG 1 cut(s) 407
XhoI CTCGAG 1 cut(s) 360
XmaI CCCGGG 2 cut(s) 340, 871
XmaJI CCTAGG 1 cut(s) 473
XmiI GTMKAC 1 cut(s) 154
Zsp2I ATGCAT 4 cut(s) 592, 1819, 2133, 2821
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.