pycom17g03480
ERF Family

2'-5' RNA ligase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0004409
Physical Location & Seq
Forward (+)
2293483 .. 2294857
1375 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 582 bp
ATGTCAGAGGGCTATGCAATCGAGCTTTACTTCGATCCAGCCCTCGAAAACCAGGTCTTGAAGGCTTGGAACGTGCTCGCCCGCCGTCAGATTAGTACCCAGCTTATCGAAATCGAATCACGACCTCACATCACCCTCTTCTCAAGTCCCTTTATTGAACCCGCCAAGCTCGAAAACGTGATCAGAACCTTCGCTTCCAAGCAAGAGCCTCTGGCCTTGTCTTTCTCTTCGATCGGGAGCCTCCCAAATGACAACAACGTCCTGTTTCTGTCGCCAACTCCGTCGGTTTCGTTGCTGCAGTTTCAGTCCCAATTGTGTGATGCAATGAAGAAAGAAGGTGTGGAAATTGGGGAGGAGTATCGCACTGACTGCTGGGTTCCTTACTGTGCTGTTGCTCAGGAAGCGCCGAAGACTCGAATGGCGGAGGCATTCTGCGTGTTGCGGGACTTGAAGTTGCCGGTTGCAGGGTATGCCATGGATATTGGATTGGTGGAGTTTTCGCCGGTTCGCGAGCTGTTCTCGTTTGTGCTAGATAATTCTGAAACCTATCTGCTTCAACAATCTAGCTATGGTTTGTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2_5_RNA_ligase2

Protein Analysis

194

Amino Acids

21.77

Weight (kDa)

4.65

Isoelectric Point (pI)

73.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2_5_RNA_ligase2 PF13563 40 - 163 1.1e-12 2'-5' RNA ligase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 257
AccII CGCG 1 cut(s) 510
AciI CCGC 4 cut(s) 82, 162, 422, 442
AclWI GGATC 1 cut(s) 29
AfaI GTAC 1 cut(s) 97
AfiI CCNNNNNNNGG 1 cut(s) 464
AgsI TTSAA 4 cut(s) 61, 158, 451, 557
AjnI CCWGG 1 cut(s) 51
AluBI AGCT 5 cut(s) 25, 103, 169, 514, 567
AluI AGCT 5 cut(s) 25, 103, 169, 514, 567
Alw21I GWGCWC 1 cut(s) 78
AlwI GGATC 1 cut(s) 29
AoxI GGCC 1 cut(s) 213
ApeKI GCWGC 1 cut(s) 295
ArsI GACNNNNNNTTYG 2 cut(s) 39, 71
AspLEI GCGC 1 cut(s) 406
AsuHPI GGTGA 1 cut(s) 124
BbsI GAAGAC 1 cut(s) 416
Bbv12I GWGCWC 1 cut(s) 78
BbvI GCAGC 1 cut(s) 282
BceAI ACGGC 1 cut(s) 69
BciT130I CCWGG 1 cut(s) 53
BclI TGATCA 1 cut(s) 180
BfaI CTAG 2 cut(s) 530, 564
BfmI CTRYAG 1 cut(s) 296
BfoI RGCGCY 1 cut(s) 407
BisI GCNGC 1 cut(s) 296
BlsI GCNGC 1 cut(s) 297
Bme1390I CCNGG 1 cut(s) 53
BmiI GGNNCC 2 cut(s) 239, 378
BmrFI CCNGG 1 cut(s) 53
BmsI GCATC 1 cut(s) 310
BpiI GAAGAC 1 cut(s) 416
BplI GAGNNNNNCTC 2 cut(s) 503, 535
Bpu10I CCTNAGC 1 cut(s) 396
BpuEI CTTGAG 1 cut(s) 127
BsaJI CCNNGG 1 cut(s) 474
Bsc4I CCNNNNNNNGG 1 cut(s) 464
Bse118I RCCGGY 2 cut(s) 457, 502
Bse3DI GCAATG 1 cut(s) 330
BseBI CCWGG 1 cut(s) 53
BseDI CCNNGG 1 cut(s) 474
BseLI CCNNNNNNNGG 1 cut(s) 464
BseMI GCAATG 1 cut(s) 330
BseMII CTCAG 1 cut(s) 410
BseRI GAGGAG 1 cut(s) 368
BseXI GCAGC 1 cut(s) 282
BseYI CCCAGC 2 cut(s) 99, 372
Bsh1236I CGCG 1 cut(s) 510
Bsh1285I CGRYCG 1 cut(s) 234
BshFI GGCC 1 cut(s) 215
BsiEI CGRYCG 1 cut(s) 234
BsiHKAI GWGCWC 1 cut(s) 78
BsiSI CCGG 2 cut(s) 458, 503
BslFI GGGAC 3 cut(s) 132, 292, 458
BslI CCNNNNNNNGG 1 cut(s) 464
BsmFI GGGAC 3 cut(s) 132, 292, 458
BsmI GAATGC 1 cut(s) 428
BsnI GGCC 1 cut(s) 215
Bsp1286I GDGCHC 1 cut(s) 78
Bsp143I GATC 3 cut(s) 34, 180, 231
Bsp19I CCATGG 1 cut(s) 474
Bsp68I TCGCGA 1 cut(s) 510
BspACI CCGC 4 cut(s) 82, 162, 422, 442
BspANI GGCC 1 cut(s) 215
BspCNI CTCAG 1 cut(s) 409
BspFNI CGCG 1 cut(s) 510
BspLI GGNNCC 2 cut(s) 239, 378
BspMAI CTGCAG 1 cut(s) 300
BspPI GGATC 1 cut(s) 29
BsrDI GCAATG 1 cut(s) 330
BsrFI RCCGGY 2 cut(s) 457, 502
BssAI RCCGGY 2 cut(s) 457, 502
BssECI CCNNGG 1 cut(s) 474
BssMI GATC 3 cut(s) 34, 180, 231
BssT1I CCWWGG 1 cut(s) 474
Bst2UI CCWGG 1 cut(s) 53
Bst4CI ACNGT 1 cut(s) 386
Bst6I CTCTTC 2 cut(s) 143, 232
BstAPI GCANNNNNTGC 2 cut(s) 369, 470
BstC8I GCNNGC 3 cut(s) 78, 82, 512
BstDEI CTNAG 1 cut(s) 396
BstDSI CCRYGG 1 cut(s) 474
BstFNI CGCG 1 cut(s) 510
BstH2I RGCGCY 1 cut(s) 407
BstHHI GCGC 1 cut(s) 406
BstKTI GATC 3 cut(s) 37, 183, 234
BstMBI GATC 3 cut(s) 34, 180, 231
BstMCI CGRYCG 1 cut(s) 234
BstMWI GCNNNNNNNGC 3 cut(s) 369, 401, 470
BstNI CCWGG 1 cut(s) 53
BstSCI CCNGG 1 cut(s) 51
BstSFI CTRYAG 1 cut(s) 296
BstUI CGCG 1 cut(s) 510
BstV1I GCAGC 1 cut(s) 282
BstV2I GAAGAC 1 cut(s) 416
BsuRI GGCC 1 cut(s) 215
BtgI CCRYGG 1 cut(s) 474
BtsIMutI CAGTG 1 cut(s) 363
BtuMI TCGCGA 1 cut(s) 510
Cac8I GCNNGC 3 cut(s) 78, 82, 512
CfoI GCGC 1 cut(s) 406
Cfr10I RCCGGY 2 cut(s) 457, 502
CsiI ACCWGGT 1 cut(s) 51
Csp6I GTAC 1 cut(s) 96
CviAII CATG 1 cut(s) 475
CviQI GTAC 1 cut(s) 96
DdeI CTNAG 1 cut(s) 396
DpnI GATC 3 cut(s) 36, 182, 233
DpnII GATC 3 cut(s) 34, 180, 231
DrdI GACNNNNNNGTC 1 cut(s) 257
DseDI GACNNNNNNGTC 1 cut(s) 257
Eam1104I CTCTTC 2 cut(s) 143, 232
EarI CTCTTC 2 cut(s) 143, 232
EciI GGCGGA 1 cut(s) 437
Eco130I CCWWGG 1 cut(s) 474
EcoRII CCWGG 1 cut(s) 51
EcoT14I CCWWGG 1 cut(s) 474
ErhI CCWWGG 1 cut(s) 474
FaeI CATG 1 cut(s) 478
FaiI YATR 4 cut(s) 15, 471, 476, 570
FaqI GGGAC 3 cut(s) 132, 292, 458
FatI CATG 1 cut(s) 474
FauI CCCGC 3 cut(s) 89, 169, 435
FbaI TGATCA 1 cut(s) 180
Fnu4HI GCNGC 1 cut(s) 296
Fsp4HI GCNGC 1 cut(s) 296
FspBI CTAG 2 cut(s) 530, 564
GlaI GCGC 1 cut(s) 405
GluI GCNGC 1 cut(s) 296
GsaI CCCAGC 2 cut(s) 103, 376
HaeII RGCGCY 1 cut(s) 407
HaeIII GGCC 1 cut(s) 215
HapII CCGG 2 cut(s) 458, 503
HhaI GCGC 1 cut(s) 406
Hin1II CATG 1 cut(s) 478
Hin6I GCGC 1 cut(s) 404
HinP1I GCGC 1 cut(s) 404
HinfI GANTC 2 cut(s) 116, 412
HpaII CCGG 2 cut(s) 458, 503
HphI GGTGA 1 cut(s) 124
Hpy188I TCNGA 4 cut(s) 7, 90, 185, 541
Hpy188III TCNNGA 5 cut(s) 58, 120, 235, 398, 509
Hpy99I CGWCG 1 cut(s) 286
HpyAV CCTTC 3 cut(s) 55, 199, 329
HpyCH4III ACNGT 1 cut(s) 386
HpyCH4IV ACGT 3 cut(s) 72, 177, 258
HpyCH4V TGCA 4 cut(s) 17, 298, 323, 464
HpyF10VI GCNNNNNNNGC 3 cut(s) 369, 401, 470
HpyF3I CTNAG 1 cut(s) 396
HpySE526I ACGT 3 cut(s) 72, 177, 258
Hsp92II CATG 1 cut(s) 478
HspAI GCGC 1 cut(s) 404
Ksp22I TGATCA 1 cut(s) 180
Kzo9I GATC 3 cut(s) 34, 180, 231
LmnI GCTCC 1 cut(s) 237
Lsp1109I GCAGC 1 cut(s) 282
LweI GCATC 1 cut(s) 310
MabI ACCWGGT 1 cut(s) 51
MaeI CTAG 2 cut(s) 530, 564
MaeII ACGT 3 cut(s) 72, 177, 258
MalI GATC 3 cut(s) 36, 182, 233
MboI GATC 3 cut(s) 34, 180, 231
MboII GAAGA 4 cut(s) 130, 219, 340, 421
MfeI CAATTG 1 cut(s) 311
MhlI GDGCHC 1 cut(s) 78
MluCI AATT 3 cut(s) 311, 345, 535
MlyI GAGTC 1 cut(s) 406
MnlI CCTC 7 cut(s) 53, 135, 146, 219, 251, 346, 418
MspI CCGG 2 cut(s) 458, 503
MspR9I CCNGG 1 cut(s) 53
MunI CAATTG 1 cut(s) 311
Mva1269I GAATGC 1 cut(s) 428
MvaI CCWGG 1 cut(s) 53
MvnI CGCG 1 cut(s) 510
MwoI GCNNNNNNNGC 3 cut(s) 369, 401, 470
NcoI CCATGG 1 cut(s) 474
NdeII GATC 3 cut(s) 34, 180, 231
NlaIII CATG 1 cut(s) 478
NlaIV GGNNCC 2 cut(s) 239, 378
NruI TCGCGA 1 cut(s) 510
PcsI WCGNNNNNNNCGW 1 cut(s) 278
PctI GAATGC 1 cut(s) 428
PfeI GAWTC 1 cut(s) 116
PkrI GCNGC 1 cut(s) 297
Ple19I CGATCG 1 cut(s) 234
PleI GAGTC 1 cut(s) 406
PpsI GAGTC 1 cut(s) 406
Psp6I CCWGG 1 cut(s) 51
PspFI CCCAGC 2 cut(s) 99, 372
PspGI CCWGG 1 cut(s) 51
PspN4I GGNNCC 2 cut(s) 239, 378
PstI CTGCAG 1 cut(s) 300
PvuI CGATCG 1 cut(s) 234
RruI TCGCGA 1 cut(s) 510
RsaI GTAC 1 cut(s) 97
RsaNI GTAC 1 cut(s) 96
SatI GCNGC 1 cut(s) 296
Sau3AI GATC 3 cut(s) 34, 180, 231
SchI GAGTC 1 cut(s) 406
ScrFI CCNGG 1 cut(s) 53
SduI GDGCHC 1 cut(s) 78
SexAI ACCWGGT 1 cut(s) 51
SfaNI GCATC 1 cut(s) 310
SfcI CTRYAG 1 cut(s) 296
SmlI CTYRAG 1 cut(s) 142
SmoI CTYRAG 1 cut(s) 142
Sse9I AATT 3 cut(s) 311, 345, 535
SsiI CCGC 4 cut(s) 82, 162, 422, 442
SspMI CTAG 2 cut(s) 530, 564
StyD4I CCNGG 1 cut(s) 51
StyI CCWWGG 1 cut(s) 474
TaaI ACNGT 1 cut(s) 386
TaiI ACGT 3 cut(s) 75, 180, 261
TaqI TCGA 8 cut(s) 21, 33, 45, 108, 114, 171, 230, 415
TasI AATT 3 cut(s) 311, 345, 535
TfiI GAWTC 1 cut(s) 116
TscAI CASTG 1 cut(s) 370
TseI GCWGC 1 cut(s) 295
TspDTI ATGAA 1 cut(s) 341
TspGWI ACGGA 1 cut(s) 270
TspRI CASTG 1 cut(s) 370
XspI CTAG 2 cut(s) 530, 564
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.