pycom17g05590

Belongs to the ClpA ClpB family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0009881
Physical Location & Seq
Reverse (-)
3980422 .. 3984105
3684 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2448 bp
ATGTTGGGACGTGATTTGGAAGCCTTGATTCAGCGAGCCAGGGATTACAAGAAAGAGTATGGGGACTCATTTGTGTCTGTCGAGCATTTGATACTTGGTTTTTCTCAAGATCAACGGTTTGGGAAGCAATTACTTCGGGATTTTCAAATATCCAAGGAAACTTTGAAATCAGCTATAGAATCCGTAAGGGGGCGCCAATCAGTTATTGACCAAGATCCTGAAGGGAAATATGAAGCACTTGAAAAGTACGGAAAAGATTTAACAGCTATGGCAAAAGTAGGAAAGCTTGACCCAGTAATAGGAAGAGATGATGAAATTCGCAGGTGCATCCAGATTCTCTCCAGAAGAACAAAGAACAACCCGGTGCTGATTGGTGAACCAGGTGTCGGAAAAACTGCAATTTCTGAAGGGCTTGCTCAAAGAATCGTGCAAGGGGATGTCCCACAAGCTCTGATGAATCGTAAGTTAATATCCCTTGACATGGGCGCACTAATTGCTGGGGCAAAGTATCGGGGAGAATTTGAGGACAGGCTGAAGGCAGTACTCAGGGAAGTAACAGAATCAGAGGGCCAGATAATCCTTTTTATCGATGAGATCCACACAGTTGTTGGAGCAGGTGCTACAAACGGTGCAATGGATGCTGGTAATCTCTTAAAACCCATGCTTGGTCGAGGAGAGTTGCGGTGTATAGGTGCAACAACACTGGATGAGTATCGTAAATATATTGAGAAAGATCCGGCATTGGAGCGTCGGTTCCAGCAAGTTTATGTTGATCAACCTACAGTGGAAGATACCATTTCAATACTCCGAGGACTGCGTGAGAGATATGAGTTGCATCATGGAGTCCGCATTTCTGATGGTGCACTGGTGGAAGCTGCAATTCTTTCAGACCGTTACATCAGTGGGCGATTTTTACCTGACAAAGCTATTGACTTAGTTGATGAAGCTGCTGCTAAATTGAAAATGGAGATTACATCAAAGCCCACAGCCCTTGATGAGATCAATCGTTCGGTGTTGAAACTTGAAATGGAGAGGTTATCTCTTACAAATGATACAGACAAAGCTTCAAAAGAGAGATTGAACCGCCTTGAAGCTGAGTTAGCTCTCTTAAAAGAAAAACAATCCGAGTTGACTGAGCAGTGGGAGCATGAAAAATCTGTCATGACTCGCATTCAGTCAATCAAGGAAGAGATTGACAGACTAAATCTTGAGATCCACCAAGCCGAACGAGAGTATGATCTCAACCGTGCAGCTGAGTTGAAGTACGGGAGTCTGAATTCTTTGCAGCGCGAACTCGTTGATGCAGAAAAAGAGCTCGATGAGTATATGAGGTCTGGGAAGTCCATGCTGAGAGAGGAAGTTAATGGAAGTGATATTGCTGAAATAGTAAGTAAGTGGACTGGTATTCCTGTTTCCAAGCTTCAACAATCGGAGAGGGAGAAGCTCTTACATTTGGAGGATGAGCTGCATAAACGTGTTGTAGGTCAGGAGCCTGCAGTGAGATCAGTGGCTGAAGCTATTCAGCGGTCTCGGGCAGGTCTCTCAGATCCTCATCGCCCCATTGCTAGTTTCATGTTTATGGGTCCCACTGGTGTTGGGAAGACAGAACTAGCTAAGGCCCTCGCCTCCTACTTGTTCAACACGGAAGAAACACTTGTAAGAATCGATATGAGTGAGTACATGGAAAAGCATGCTGTTTCAAGATTGATAGGAGCTCCACCTGGTTATGTGGGGTATGAGGAGGGAGGACAGCTGACAGAGGTGGTTCGCCGGAGACCATATTCAGTTATTTTGTTCGACGAGATAGAGAAGGCACATTCTGATGTGTTCAATGTGTTCCTTCAAATTTTAGACGATGGGAGAATAACTGACTCGCAGGGTCGTACTGTGAGCTTCACCAACACTGTCATCATTATGACCTCAAATGTGGGTTCACAGTACATACTAAATGCTGACGATGACGACACTCCTAAAGAGTTGGCTTACGACACTATAAAGCACCGGGTACTGGAGGCTGCAAGATCAATATTTCGCCCCGAGTTCATGAACCGTGTTGATGAGTACATAGTTTTCCAGCCCCTGGACCGTGATCAGATAAGCCGTATTGTCAAGCTACAGTTGGACCGAGTGCAAAAGAGAATTGCAGACCGCAAGATGAAGATCAAGGTGAGCGATGCAGCTATCGAGCTTCTCGGAAGTCTTGGGTATGATCCAAACTACGGCGCCAGGCCAGTGAAGCGGGTGATACAGCAGTATATCGAAAACGAGCTTGCCAAGGGCATACTGAGAGGAGAGTTTAGGGAAGAAGACACAGTGGCCATAGACACAGAGGTGACGACATTTTCCAGTGGCCAGCTCCCCCAGCAAAAGCTAGTGTTCAGGAGGTTGAAAACTGACTCAGAATCTCCGGCTACACAGAACCAAGAGGCTTTCTCGGAGGCTCGGTAG

Protein Analysis

816

Amino Acids

92.29

Weight (kDa)

5.48

Isoelectric Point (pI)

38.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Clp_N PF02861 2 - 47 2e-06 Clp repeat (R) N-terminal domain
NBD_SMAX1 PF23569 104 - 206 3.6e-06 SMAX1 nucleotide binding domain
AAA PF00004 122 - 253 2e-12 ATPase family associated with various cellular activities (AAA)
AAA_lid_9 PF17871 261 - 363 5.6e-34 AAA lid domain
Sigma54_activat PF00158 516 - 642 4.1e-06 Sigma-54 interaction domain
AAA_2 PF07724 519 - 687 4.3e-69 AAA domain (Cdc48 subfamily)
AAA_5 PF07728 524 - 645 9.7e-12 AAA domain (dynein-related subfamily)
AAA PF00004 525 - 641 1.9e-09 ATPase family associated with various cellular activities (AAA)
ClpB_D2-small PF10431 694 - 774 1.8e-27 C-terminal, D2-small domain, of ClpB protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 312, 605
Acc36I ACCTGC 3 cut(s) 312, 605, 1526
AccB1I GGYRCC 2 cut(s) 192, 2222
AccB7I CCANNNNNTGG 1 cut(s) 2080
AccII CGCG 1 cut(s) 1290
AciI CCGC 6 cut(s) 682, 847, 1084, 1525, 2149, 2239
AclWI GGATC 6 cut(s) 209, 589, 728, 1207, 1541, 2204
AcoI YGGCCR 2 cut(s) 2316, 2350
AcsI RAATTY 4 cut(s) 315, 518, 1276, 1845
AcuI CTGAAG 4 cut(s) 240, 426, 554, 1533
AcyI GRCGYC 2 cut(s) 193, 2223
AfaI GTAC 8 cut(s) 248, 543, 1265, 1679, 1885, 1940, 2007, 2063
AfiI CCNNNNNNNGG 8 cut(s) 189, 299, 386, 481, 665, 2008, 2080, 2219
AflIII ACRYGT 1 cut(s) 1474
AjiI CACGTC 1 cut(s) 11
AjnI CCWGG 5 cut(s) 38, 379, 1720, 2079, 2225
AleI CACNNNNGTG 1 cut(s) 2330
AloI GAACNNNNNNTCC 2 cut(s) 737, 769
Alw21I GWGCWC 3 cut(s) 865, 1317, 1717
Alw26I GTCTC 3 cut(s) 1533, 1544, 1768
Alw44I GTGCAC 1 cut(s) 861
AlwI GGATC 6 cut(s) 209, 589, 728, 1207, 1541, 2204
AlwNI CAGNNNCTG 2 cut(s) 1511, 2080
Ama87I CYCGRG 2 cut(s) 1530, 2036
AoxI GGCC 5 cut(s) 568, 1617, 2228, 2316, 2350
ApaLI GTGCAC 1 cut(s) 861
ApeKI GCWGC 8 cut(s) 875, 947, 950, 1250, 1285, 1465, 2015, 2177
ApoI RAATTY 4 cut(s) 315, 518, 1276, 1845
Asp700I GAANNNNTTC 1 cut(s) 1518
AspLEI GCGC 4 cut(s) 195, 488, 1290, 2225
AspS9I GGNCC 5 cut(s) 568, 1583, 1618, 2083, 2122
AsuC2I CCSGG 2 cut(s) 362, 2003
AsuHPI GGTGA 5 cut(s) 386, 1888, 2179, 2254, 2344
AvaI CYCGRG 2 cut(s) 1530, 2036
AvaII GGWCC 3 cut(s) 1583, 2083, 2122
BaeGI GKGCMC 1 cut(s) 865
BaeI ACNNNNGTAYC 2 cut(s) 2237, 2270
BalI TGGCCA 2 cut(s) 2318, 2352
BanI GGYRCC 2 cut(s) 192, 2222
BanII GRGCYC 2 cut(s) 1317, 1717
BbsI GAAGAC 2 cut(s) 1607, 2313
Bbv12I GWGCWC 3 cut(s) 865, 1317, 1717
BbvI GCAGC 8 cut(s) 862, 934, 937, 1262, 1297, 1452, 2002, 2189
BccI CCATC 2 cut(s) 851, 1850
BceAI ACGGC 2 cut(s) 2085, 2236
BcgI CGANNNNNNTGC 2 cut(s) 116, 150
BciT130I CCWGG 5 cut(s) 40, 381, 1722, 2081, 2227
BclI TGATCA 2 cut(s) 772, 2089
BcnI CCSGG 2 cut(s) 362, 2003
BcoDI GTCTC 3 cut(s) 1533, 1544, 1768
BfaI CTAG 3 cut(s) 1566, 1610, 2372
BfmI CTRYAG 4 cut(s) 174, 780, 1494, 2114
BfoI RGCGCY 2 cut(s) 196, 2226
BfuAI ACCTGC 3 cut(s) 312, 605, 1526
BisI GCNGC 8 cut(s) 876, 948, 951, 1251, 1286, 1466, 2016, 2178
BlsI GCNGC 8 cut(s) 877, 949, 952, 1252, 1287, 1467, 2017, 2179
BmcAI AGTACT 1 cut(s) 543
Bme1390I CCNGG 7 cut(s) 40, 362, 381, 1722, 2003, 2081, 2227
Bme18I GGWCC 3 cut(s) 1583, 2083, 2122
BmeT110I CYCGRG 2 cut(s) 1530, 2036
BmgBI CACGTC 1 cut(s) 11
BmgT120I GGNCC 5 cut(s) 568, 1583, 1618, 2083, 2122
BmiI GGNNCC 6 cut(s) 194, 755, 1491, 1584, 1585, 2224
BmrFI CCNGG 7 cut(s) 40, 362, 381, 1722, 2003, 2081, 2227
BmrI ACTGGG 1 cut(s) 287
BmsI GCATC 5 cut(s) 336, 628, 844, 1291, 2164
BmuI ACTGGG 1 cut(s) 287
BpiI GAAGAC 2 cut(s) 1607, 2313
BplI GAGNNNNNCTC 3 cut(s) 1024, 1056, 2417
BpmI CTGGAG 2 cut(s) 325, 2030
Bpu10I CCTNAGC 1 cut(s) 1614
BpuEI CTTGAG 2 cut(s) 90, 1229
BpuMI CCSGG 2 cut(s) 362, 2003
Bsa29I ATCGAT 2 cut(s) 588, 1665
BsaBI GATNNNNATC 3 cut(s) 711, 1551, 2159
BsaHI GRCGYC 2 cut(s) 193, 2223
BsaI GGTCTC 3 cut(s) 1533, 1544, 1768
BsaJI CCNNGG 5 cut(s) 39, 153, 808, 2079, 2274
BsaXI ACNNNNNCTCC 3 cut(s) 737, 767, 2429
Bsc4I CCNNNNNNNGG 8 cut(s) 189, 299, 386, 481, 665, 2008, 2080, 2219
Bse1I ACTGG 8 cut(s) 293, 708, 870, 1405, 1594, 2013, 2231, 2346
Bse3DI GCAATG 2 cut(s) 639, 1560
Bse8I GATNNNNATC 3 cut(s) 711, 1551, 2159
BseBI CCWGG 5 cut(s) 40, 381, 1722, 2081, 2227
BseCI ATCGAT 2 cut(s) 588, 1665
BseDI CCNNGG 5 cut(s) 39, 153, 808, 2079, 2274
BseGI GGATG 5 cut(s) 327, 442, 643, 712, 1465
BseJI GATNNNNATC 3 cut(s) 711, 1551, 2159
BseLI CCNNNNNNNGG 8 cut(s) 189, 299, 386, 481, 665, 2008, 2080, 2219
BseMI GCAATG 2 cut(s) 639, 1560
BseMII CTCAG 8 cut(s) 559, 1086, 1125, 1245, 1340, 1557, 2276, 2412
BseNI ACTGG 8 cut(s) 293, 708, 870, 1405, 1594, 2013, 2231, 2346
BseRI GAGGAG 3 cut(s) 687, 1754, 2304
BseSI GKGCMC 1 cut(s) 865
BseXI GCAGC 8 cut(s) 862, 934, 937, 1262, 1297, 1452, 2002, 2189
BseYI CCCAGC 2 cut(s) 497, 2361
BsgI GTGCAG 1 cut(s) 1269
Bsh1236I CGCG 1 cut(s) 1290
BshFI GGCC 5 cut(s) 570, 1619, 2230, 2318, 2352
BshNI GGYRCC 2 cut(s) 192, 2222
BshVI ATCGAT 2 cut(s) 588, 1665
BsiHKAI GWGCWC 3 cut(s) 865, 1317, 1717
BsiHKCI CYCGRG 2 cut(s) 1530, 2036
BsiSI CCGG 5 cut(s) 362, 737, 1771, 2002, 2408
BslFI GGGAC 4 cut(s) 21, 77, 425, 1569
BslI CCNNNNNNNGG 8 cut(s) 189, 299, 386, 481, 665, 2008, 2080, 2219
BsmAI GTCTC 3 cut(s) 1533, 1544, 1768
BsmFI GGGAC 4 cut(s) 21, 77, 425, 1569
BsmI GAATGC 1 cut(s) 1170
BsnI GGCC 5 cut(s) 570, 1619, 2230, 2318, 2352
Bso31I GGTCTC 3 cut(s) 1533, 1544, 1768
BsoBI CYCGRG 2 cut(s) 1530, 2036
Bsp1286I GDGCHC 3 cut(s) 865, 1317, 1717
BspACI CCGC 6 cut(s) 682, 847, 1084, 1525, 2149, 2239
BspANI GGCC 5 cut(s) 570, 1619, 2230, 2318, 2352
BspCNI CTCAG 8 cut(s) 558, 1087, 1126, 1246, 1341, 1556, 2277, 2411
BspDI ATCGAT 2 cut(s) 588, 1665
BspFNI CGCG 1 cut(s) 1290
BspHI TCATGA 2 cut(s) 1161, 2043
BspLI GGNNCC 6 cut(s) 194, 755, 1491, 1584, 1585, 2224
BspMAI CTGCAG 1 cut(s) 1498
BspMI ACCTGC 3 cut(s) 312, 605, 1526
BspPI GGATC 6 cut(s) 209, 589, 728, 1207, 1541, 2204
BspT107I GGYRCC 2 cut(s) 192, 2222
BspTNI GGTCTC 3 cut(s) 1533, 1544, 1768
BsrDI GCAATG 2 cut(s) 639, 1560
BsrI ACTGG 8 cut(s) 293, 708, 870, 1405, 1594, 2013, 2231, 2346
BssECI CCNNGG 5 cut(s) 39, 153, 808, 2079, 2274
BssNI GRCGYC 2 cut(s) 193, 2223
BssT1I CCWWGG 2 cut(s) 153, 2274
Bst2UI CCWGG 5 cut(s) 40, 381, 1722, 2081, 2227
Bst6I CTCTTC 2 cut(s) 298, 1182
BstACI GRCGYC 2 cut(s) 193, 2223
BstAPI GCANNNNNTGC 2 cut(s) 494, 638
BstC8I GCNNGC 6 cut(s) 36, 414, 1494, 1692, 2271, 2354
BstF5I GGATG 5 cut(s) 327, 442, 643, 712, 1465
BstFNI CGCG 1 cut(s) 1290
BstH2I RGCGCY 2 cut(s) 196, 2226
BstHHI GCGC 4 cut(s) 195, 488, 1290, 2225
BstMAI GTCTC 3 cut(s) 1533, 1544, 1768
BstMWI GCNNNNNNNGC 6 cut(s) 494, 638, 1100, 1144, 2236, 2362
BstNI CCWGG 5 cut(s) 40, 381, 1722, 2081, 2227
BstNSI RCATGY 1 cut(s) 1694
BstSCI CCNGG 7 cut(s) 38, 360, 379, 1720, 2001, 2079, 2225
BstSFI CTRYAG 4 cut(s) 174, 780, 1494, 2114
BstSLI GKGCMC 1 cut(s) 865
BstUI CGCG 1 cut(s) 1290
BstV1I GCAGC 8 cut(s) 862, 934, 937, 1262, 1297, 1452, 2002, 2189
BstV2I GAAGAC 2 cut(s) 1607, 2313
BstX2I RGATCY 5 cut(s) 214, 594, 733, 1212, 1546
BstYI RGATCY 5 cut(s) 214, 594, 733, 1212, 1546
Bsu15I ATCGAT 2 cut(s) 588, 1665
BsuRI GGCC 5 cut(s) 570, 1619, 2230, 2318, 2352
BsuTUI ATCGAT 2 cut(s) 588, 1665
BtgZI GCGATG 2 cut(s) 1538, 2187
BtrI CACGTC 1 cut(s) 11
BtsCI GGATG 5 cut(s) 327, 442, 643, 712, 1465
BtsI GCAGTG 2 cut(s) 1145, 1503
BveI ACCTGC 3 cut(s) 312, 605, 1526
Cac8I GCNNGC 6 cut(s) 36, 414, 1494, 1692, 2271, 2354
CaiI CAGNNNCTG 2 cut(s) 1511, 2080
CciI TCATGA 2 cut(s) 1161, 2043
CfoI GCGC 4 cut(s) 195, 488, 1290, 2225
Cfr13I GGNCC 5 cut(s) 568, 1583, 1618, 2083, 2122
ClaI ATCGAT 2 cut(s) 588, 1665
CseI GACGC 1 cut(s) 737
CsiI ACCWGGT 2 cut(s) 379, 1720
Csp6I GTAC 8 cut(s) 247, 542, 1264, 1678, 1884, 1939, 2006, 2062
CspCI CAANNNNNGTGG 4 cut(s) 973, 1008, 1576, 1611
CviQI GTAC 8 cut(s) 247, 542, 1264, 1678, 1884, 1939, 2006, 2062
DinI GGCGCC 2 cut(s) 194, 2224
EaeI YGGCCR 2 cut(s) 2316, 2350
Eam1104I CTCTTC 2 cut(s) 298, 1182
EarI CTCTTC 2 cut(s) 298, 1182
Ecl136II GAGCTC 2 cut(s) 1315, 1715
Eco130I CCWWGG 2 cut(s) 153, 2274
Eco24I GRGCYC 2 cut(s) 1317, 1717
Eco31I GGTCTC 3 cut(s) 1533, 1544, 1768
Eco47I GGWCC 3 cut(s) 1583, 2083, 2122
Eco53kI GAGCTC 2 cut(s) 1315, 1715
Eco57I CTGAAG 4 cut(s) 240, 426, 554, 1533
Eco88I CYCGRG 2 cut(s) 1530, 2036
EcoICRI GAGCTC 2 cut(s) 1315, 1715
EcoO109I RGGNCCY 2 cut(s) 1583, 1618
EcoRI GAATTC 1 cut(s) 1276
EcoRII CCWGG 5 cut(s) 38, 379, 1720, 2079, 2225
EcoT14I CCWWGG 2 cut(s) 153, 2274
EcoT38I GRGCYC 2 cut(s) 1317, 1717
EgeI GGCGCC 2 cut(s) 194, 2224
EheI GGCGCC 2 cut(s) 194, 2224
ErhI CCWWGG 2 cut(s) 153, 2274
FalI AAGNNNNNCTT 2 cut(s) 1638, 1670
FaqI GGGAC 4 cut(s) 21, 77, 425, 1569
FauI CCCGC 1 cut(s) 2232
FbaI TGATCA 2 cut(s) 772, 2089
Fnu4HI GCNGC 8 cut(s) 876, 948, 951, 1251, 1286, 1466, 2016, 2178
FokI GGATG 5 cut(s) 314, 449, 650, 719, 1472
FriOI GRGCYC 2 cut(s) 1317, 1717
Fsp4HI GCNGC 8 cut(s) 876, 948, 951, 1251, 1286, 1466, 2016, 2178
FspBI CTAG 3 cut(s) 1566, 1610, 2372
GlaI GCGC 4 cut(s) 194, 487, 1289, 2224
GluI GCNGC 8 cut(s) 876, 948, 951, 1251, 1286, 1466, 2016, 2178
GsaI CCCAGC 2 cut(s) 501, 2365
GsuI CTGGAG 2 cut(s) 325, 2030
HaeII RGCGCY 2 cut(s) 196, 2226
HaeIII GGCC 5 cut(s) 570, 1619, 2230, 2318, 2352
HapII CCGG 5 cut(s) 362, 737, 1771, 2002, 2408
HgaI GACGC 1 cut(s) 737
HhaI GCGC 4 cut(s) 195, 488, 1290, 2225
Hin1I GRCGYC 2 cut(s) 193, 2223
Hin6I GCGC 4 cut(s) 193, 486, 1288, 2223
HinP1I GCGC 4 cut(s) 193, 486, 1288, 2223
HincII GTYRAC 1 cut(s) 1131
HindII GTYRAC 1 cut(s) 1131
HindIII AAGCTT 3 cut(s) 284, 1062, 1418
HpaII CCGG 5 cut(s) 362, 737, 1771, 2002, 2408
HphI GGTGA 5 cut(s) 386, 1888, 2179, 2254, 2344
Hpy166II GTNNAC 5 cut(s) 377, 863, 1131, 1398, 1934
Hpy8I GTNNAC 5 cut(s) 377, 863, 1131, 1398, 1934
Hpy99I CGWCG 2 cut(s) 753, 1802
HpyAV CCTTC 5 cut(s) 215, 401, 529, 1804, 1850
HpyCH4IV ACGT 2 cut(s) 10, 1474
HpyF10VI GCNNNNNNNGC 6 cut(s) 494, 638, 1100, 1144, 2236, 2362
HpySE526I ACGT 2 cut(s) 10, 1474
Hsp92I GRCGYC 2 cut(s) 193, 2223
HspAI GCGC 4 cut(s) 193, 486, 1288, 2223
KasI GGCGCC 2 cut(s) 192, 2222
KflI GGGWCCC 1 cut(s) 1583
Ksp22I TGATCA 2 cut(s) 772, 2089
LmnI GCTCC 7 cut(s) 611, 745, 1144, 1489, 1712, 1720, 2361
Lsp1109I GCAGC 8 cut(s) 862, 934, 937, 1262, 1297, 1452, 2002, 2189
LweI GCATC 5 cut(s) 336, 628, 844, 1291, 2164
MabI ACCWGGT 2 cut(s) 379, 1720
MaeI CTAG 3 cut(s) 1566, 1610, 2372
MaeII ACGT 2 cut(s) 10, 1474
MaeIII GTNAC 3 cut(s) 553, 893, 2332
MboII GAAGA 9 cut(s) 315, 357, 800, 1199, 1612, 1658, 2170, 2315, 2318
MflI RGATCY 5 cut(s) 214, 594, 733, 1212, 1546
MhlI GDGCHC 3 cut(s) 865, 1317, 1717
MlsI TGGCCA 2 cut(s) 2318, 2352
MluNI TGGCCA 2 cut(s) 2318, 2352
Mly113I GGCGCC 2 cut(s) 193, 2223
MlyI GAGTC 6 cut(s) 59, 852, 1159, 1279, 1865, 2390
MmeI TCCRAC 3 cut(s) 367, 589, 2100
Mox20I TGGCCA 2 cut(s) 2318, 2352
MroXI GAANNNNTTC 1 cut(s) 1518
MscI TGGCCA 2 cut(s) 2318, 2352
MseI TTAA 5 cut(s) 260, 467, 653, 1109, 1362
MslI CAYNNNNRTG 5 cut(s) 1473, 1577, 1821, 1913, 2330
Msp20I TGGCCA 2 cut(s) 2318, 2352
MspA1I CMGCKG 3 cut(s) 1253, 1525, 1753
MspI CCGG 5 cut(s) 362, 737, 1771, 2002, 2408
MspR9I CCNGG 7 cut(s) 40, 362, 381, 1722, 2003, 2081, 2227
Mva1269I GAATGC 1 cut(s) 1170
MvaI CCWGG 5 cut(s) 40, 381, 1722, 2081, 2227
MvnI CGCG 1 cut(s) 1290
MwoI GCNNNNNNNGC 6 cut(s) 494, 638, 1100, 1144, 2236, 2362
NarI GGCGCC 2 cut(s) 193, 2223
NciI CCSGG 2 cut(s) 362, 2003
NlaIV GGNNCC 6 cut(s) 194, 755, 1491, 1584, 1585, 2224
NmuCI GTSAC 1 cut(s) 2332
NspI RCATGY 1 cut(s) 1694
OliI CACNNNNGTG 1 cut(s) 2330
PaeI GCATGC 1 cut(s) 1694
PagI TCATGA 2 cut(s) 1161, 2043
PaqCI CACCTGC 2 cut(s) 312, 605
PctI GAATGC 1 cut(s) 1170
PdmI GAANNNNTTC 1 cut(s) 1518
PfeI GAWTC 8 cut(s) 28, 179, 334, 423, 457, 560, 1662, 2402
PflMI CCANNNNNTGG 1 cut(s) 2080
PkrI GCNGC 8 cut(s) 877, 949, 952, 1252, 1287, 1467, 2017, 2179
PleI GAGTC 6 cut(s) 59, 851, 1159, 1278, 1865, 2390
PluTI GGCGCC 2 cut(s) 196, 2226
PpsI GAGTC 6 cut(s) 59, 851, 1159, 1278, 1865, 2390
PpuMI RGGWCCY 1 cut(s) 1583
Psp124BI GAGCTC 2 cut(s) 1317, 1717
Psp5II RGGWCCY 1 cut(s) 1583
Psp6I CCWGG 5 cut(s) 38, 379, 1720, 2079, 2225
PspFI CCCAGC 2 cut(s) 497, 2361
PspGI CCWGG 5 cut(s) 38, 379, 1720, 2079, 2225
PspN4I GGNNCC 6 cut(s) 194, 755, 1491, 1584, 1585, 2224
PspPI GGNCC 5 cut(s) 568, 1583, 1618, 2083, 2122
PspPPI RGGWCCY 1 cut(s) 1583
PstI CTGCAG 1 cut(s) 1498
PstNI CAGNNNCTG 2 cut(s) 1511, 2080
PsuI RGATCY 5 cut(s) 214, 594, 733, 1212, 1546
PvuII CAGCTG 2 cut(s) 1253, 1753
RsaI GTAC 8 cut(s) 248, 543, 1265, 1679, 1885, 1940, 2007, 2063
RsaNI GTAC 8 cut(s) 247, 542, 1264, 1678, 1884, 1939, 2006, 2062
RseI CAYNNNNRTG 5 cut(s) 1473, 1577, 1821, 1913, 2330
SacI GAGCTC 2 cut(s) 1317, 1717
SaqAI TTAA 5 cut(s) 260, 467, 653, 1109, 1362
SatI GCNGC 8 cut(s) 876, 948, 951, 1251, 1286, 1466, 2016, 2178
Sau96I GGNCC 5 cut(s) 568, 1583, 1618, 2083, 2122
ScaI AGTACT 1 cut(s) 543
SchI GAGTC 6 cut(s) 59, 852, 1159, 1279, 1865, 2390
ScrFI CCNGG 7 cut(s) 40, 362, 381, 1722, 2003, 2081, 2227
SduI GDGCHC 3 cut(s) 865, 1317, 1717
SexAI ACCWGGT 2 cut(s) 379, 1720
SfaNI GCATC 5 cut(s) 336, 628, 844, 1291, 2164
SfcI CTRYAG 4 cut(s) 174, 780, 1494, 2114
SfoI GGCGCC 2 cut(s) 194, 2224
SinI GGWCC 3 cut(s) 1583, 2083, 2122
SmiMI CAYNNNNRTG 5 cut(s) 1473, 1577, 1821, 1913, 2330
SmlI CTYRAG 2 cut(s) 105, 1208
SmoI CTYRAG 2 cut(s) 105, 1208
SphI GCATGC 1 cut(s) 1694
SsiI CCGC 6 cut(s) 682, 847, 1084, 1525, 2149, 2239
SspDI GGCGCC 2 cut(s) 192, 2222
SspI AATATT 1 cut(s) 2028
SspMI CTAG 3 cut(s) 1566, 1610, 2372
SstI GAGCTC 2 cut(s) 1317, 1717
StyD4I CCNGG 7 cut(s) 38, 360, 379, 1720, 2001, 2079, 2225
StyI CCWWGG 2 cut(s) 153, 2274
TaiI ACGT 2 cut(s) 13, 1477
TaqI TCGA 8 cut(s) 81, 588, 670, 1317, 1665, 1797, 2184, 2259
TaqII GACCGA 1 cut(s) 2139
TatI WGTACW 4 cut(s) 541, 1677, 1938, 2061
TfiI GAWTC 8 cut(s) 28, 179, 334, 423, 457, 560, 1662, 2402
Tru1I TTAA 5 cut(s) 260, 467, 653, 1109, 1362
Tru9I TTAA 5 cut(s) 260, 467, 653, 1109, 1362
TseFI GTSAC 1 cut(s) 2332
TseI GCWGC 8 cut(s) 875, 947, 950, 1250, 1285, 1465, 2015, 2177
Tsp45I GTSAC 1 cut(s) 2332
TspDTI ATGAA 9 cut(s) 246, 327, 470, 957, 1164, 1561, 2032, 2060, 2171
TspGWI ACGGA 3 cut(s) 172, 264, 1658
Van91I CCANNNNNTGG 1 cut(s) 2080
VneI GTGCAC 1 cut(s) 861
VpaK11BI GGWCC 3 cut(s) 1583, 2083, 2122
XapI RAATTY 4 cut(s) 315, 518, 1276, 1845
XceI RCATGY 1 cut(s) 1694
XcmI CCANNNNNNNNNTGG 1 cut(s) 605
XmnI GAANNNNTTC 1 cut(s) 1518
XspI CTAG 3 cut(s) 1566, 1610, 2372
ZrmI AGTACT 1 cut(s) 543
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.