pycom17g08360

GPI-anchored protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0011246
Physical Location & Seq
Forward (+)
6214239 .. 6215310
1072 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 987 bp
ATGTCATCATTTCTTCCCACCTCCACTCTCACATTTTTCCTCCTCCTCCTCCTCTTATTAATATCCAGCTTTCATATTTCCCTCGCCGGTCTACTCGTCGAACCAGTGACCGGTCACCCCCAGCCGCTCAAACCGGGGGAATACTCCAGCCCCAACACCATCCCGGCCCTCCCGTCCCAAACCCAGACCCAAACCTGCCACCTCGACCTCTCCGCGGAGCTCTTCGGCGGCGTCAGCCAAGCATGCGGCCGAGACCTCGACCGGAGCCGGTGCTGCCCTGTTTTAGCCGCCTGGCTCTTCGCCGCCCACGCCGGATCAGCCCTCGAGCTCCCCTCGTCGGATACGGCTCCGTCGGCGGTGCCCGACCAGCCGATGATGCCGGACGACTCGCAGAAGTGCGTCAACTCGCTGCAGTCCTCGCTCGTCAGCCGGAACATTAGGATTCCTCAGCCCAACGCCAGCTGCGACGCGGTTCTGTGCTTCTGCGGAATCCGGCTCCACCAGATCGGCTCCTTGAGCTGCCCCGCCGCCTTCAACGTGTCGCGAGGATTTCACAACGCCACCCCCACCGCCGCTGTCAGGAATCTCGAGAAGAACTGCCGCAACTCTTCGTACTCTGGATGCACCAAGTGCCTGGGCGCTCTCCAGAAGCTCAAGGGAGGGAGCAAGAACGGAACGGCGGGGGACAGGAGCACGAGCGAGAGAGCGAACAAGATGTTCAACCTTGACTGCCAGCTAATGGGCCTGACGTGGCTCCTCGCCCGGAACAAAACGGCGTACATTCCGACCGTTTCGGCCGTGCTGCGCGCCATAATGTACAGCGCGCACCCGCCGCACGAGAACACGTGTAGCCCGGACCAGGAGAACATGCCGCTGGCCGTGGATTCGCTGCAGATCGAGAACGGGCAAGGTTTCGCCGCATCGCCGCCGTCCATCTTCTTGTTACCGATTCTCCCTCTGATCATTTGGGCGTCTCTGTTTGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0031224 GO:0031225 GO:0031226 GO:0044425 GO:0044459 GO:0044464 GO:0046658 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

329

Amino Acids

35.07

Weight (kDa)

7.51

Isoelectric Point (pI)

58.29

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011246)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G28100
fragaria_vesca FvH4_6g44390
malus_domestica MD09G1097800.v1.1 MD17G1086100.v1.1
prunus_persica Prupe.3G228400_v2.0.a1
pyrus_communis pycom09g02190 pycom17g08360
rosa_chinensis RchiOBHm_Chr2g0161331 RchiOBHm_Chr2g0161381
rosa_laevigata RLG00000021280
rosa_multiflora Rmu_co8294437.1_g000001 Rmu_ssc0000196.1_g000002
rosa_roxburghii Rroxscaffold_2G00088890
rosa_rugosa Rorug02G0488500
rosa_samantha Rh2AG555000 Rh2BG568200 Rh2CG538700 Rh2CG538800 Rh2DG577900
rosa_wichuraiana Rw2G045920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 203
AccB1I GGYRCC 1 cut(s) 358
AccB7I CCANNNNNTGG 1 cut(s) 739
AccBSI CCGCTC 1 cut(s) 127
AccI GTMKAC 1 cut(s) 91
AccII CGCG 5 cut(s) 215, 470, 544, 807, 824
AclWI GGATC 1 cut(s) 322
AcoI YGGCCR 3 cut(s) 247, 795, 876
AcvI CACGTG 1 cut(s) 846
AcyI GRCGYC 2 cut(s) 231, 971
AdeI CACNNNGTG 1 cut(s) 630
AfaI GTAC 3 cut(s) 614, 779, 818
AfiI CCNNNNNNNGG 6 cut(s) 110, 214, 337, 579, 739, 859
AflIII ACRYGT 3 cut(s) 537, 843, 845
AgeI ACCGGT 1 cut(s) 110
AgsI TTSAA 2 cut(s) 535, 721
AjiI CACGTC 1 cut(s) 750
AjnI CCWGG 3 cut(s) 290, 633, 858
AluBI AGCT 7 cut(s) 69, 220, 328, 462, 519, 652, 736
AluI AGCT 7 cut(s) 69, 220, 328, 462, 519, 652, 736
Alw21I GWGCWC 3 cut(s) 222, 330, 695
Alw26I GTCTC 2 cut(s) 246, 978
AlwI GGATC 1 cut(s) 322
Ama87I CYCGRG 2 cut(s) 323, 587
AoxI GGCC 5 cut(s) 165, 247, 742, 795, 876
ApeKI GCWGC 6 cut(s) 273, 409, 462, 519, 802, 889
AseI ATTAAT 1 cut(s) 59
AsiGI ACCGGT 1 cut(s) 110
AspLEI GCGC 5 cut(s) 641, 807, 809, 824, 826
AspS9I GGNCC 3 cut(s) 166, 742, 856
AsuC2I CCSGG 4 cut(s) 135, 164, 763, 854
AsuHPI GGTGA 1 cut(s) 107
AvaI CYCGRG 2 cut(s) 323, 587
AvaII GGWCC 1 cut(s) 856
BaeGI GKGCMC 1 cut(s) 363
BanI GGYRCC 1 cut(s) 358
BanII GRGCYC 2 cut(s) 222, 330
BauI CACGAG 2 cut(s) 694, 836
BbrPI CACGTG 1 cut(s) 846
Bbv12I GWGCWC 3 cut(s) 222, 330, 695
BbvCI CCTCAGC 1 cut(s) 447
BbvI GCAGC 6 cut(s) 260, 396, 449, 506, 789, 876
BccI CCATC 2 cut(s) 167, 941
BceAI ACGGC 6 cut(s) 360, 693, 782, 789, 863, 913
BciT130I CCWGG 3 cut(s) 292, 635, 860
BciVI GTATCC 1 cut(s) 334
BclI TGATCA 1 cut(s) 960
BcnI CCSGG 4 cut(s) 135, 164, 763, 854
BcoDI GTCTC 2 cut(s) 246, 978
BfmI CTRYAG 2 cut(s) 410, 890
BfoI RGCGCY 1 cut(s) 642
BfuAI ACCTGC 1 cut(s) 203
BfuI GTATCC 1 cut(s) 334
Bme1390I CCNGG 7 cut(s) 135, 164, 292, 635, 763, 854, 860
Bme18I GGWCC 1 cut(s) 856
BmeT110I CYCGRG 2 cut(s) 323, 587
BmgBI CACGTC 1 cut(s) 750
BmgT120I GGNCC 3 cut(s) 166, 742, 856
BmiI GGNNCC 6 cut(s) 266, 348, 360, 497, 511, 755
BmrFI CCNGG 7 cut(s) 135, 164, 292, 635, 763, 854, 860
BmsI GCATC 3 cut(s) 366, 611, 929
BplI GAGNNNNNCTC 2 cut(s) 317, 349
BpmI CTGGAG 2 cut(s) 130, 629
Bpu10I CCTNAGC 1 cut(s) 447
BpuEI CTTGAG 2 cut(s) 535, 638
BpuMI CCSGG 4 cut(s) 135, 164, 763, 854
BsaAI YACGTR 1 cut(s) 846
BsaHI GRCGYC 2 cut(s) 231, 971
BsaI GGTCTC 1 cut(s) 246
BsaJI CCNNGG 4 cut(s) 134, 213, 634, 879
BsaWI WCCGGW 2 cut(s) 110, 261
BsaXI ACNNNNNCTCC 2 cut(s) 936, 966
Bsc4I CCNNNNNNNGG 6 cut(s) 110, 214, 337, 579, 739, 859
Bse118I RCCGGY 3 cut(s) 86, 110, 267
Bse1I ACTGG 1 cut(s) 104
BseBI CCWGG 3 cut(s) 292, 635, 860
BseDI CCNNGG 4 cut(s) 134, 213, 634, 879
BseGI GGATG 2 cut(s) 159, 626
BseLI CCNNNNNNNGG 6 cut(s) 110, 214, 337, 579, 739, 859
BseMII CTCAG 1 cut(s) 461
BseNI ACTGG 1 cut(s) 104
BsePI GCGCGC 2 cut(s) 805, 822
BseRI GAGGAG 5 cut(s) 32, 35, 38, 41, 746
BseSI GKGCMC 1 cut(s) 363
BseX3I CGGCCG 2 cut(s) 247, 795
BseXI GCAGC 6 cut(s) 260, 396, 449, 506, 789, 876
BseYI CCCAGC 1 cut(s) 120
Bsh1236I CGCG 5 cut(s) 215, 470, 544, 807, 824
Bsh1285I CGRYCG 4 cut(s) 250, 262, 789, 798
BshFI GGCC 5 cut(s) 167, 249, 744, 797, 878
BshNI GGYRCC 1 cut(s) 358
BshTI ACCGGT 1 cut(s) 110
BsiEI CGRYCG 4 cut(s) 250, 262, 789, 798
BsiHKAI GWGCWC 3 cut(s) 222, 330, 695
BsiHKCI CYCGRG 2 cut(s) 323, 587
BslFI GGGAC 2 cut(s) 160, 698
BslI CCNNNNNNNGG 6 cut(s) 110, 214, 337, 579, 739, 859
BsmAI GTCTC 2 cut(s) 246, 978
BsmBI CGTCTC 1 cut(s) 978
BsmFI GGGAC 2 cut(s) 160, 698
BsnI GGCC 5 cut(s) 167, 249, 744, 797, 878
Bso31I GGTCTC 1 cut(s) 246
BsoBI CYCGRG 2 cut(s) 323, 587
Bsp1286I GDGCHC 4 cut(s) 222, 330, 363, 695
Bsp1407I TGTACA 1 cut(s) 816
Bsp143I GATC 4 cut(s) 314, 504, 894, 960
Bsp68I TCGCGA 1 cut(s) 544
BspANI GGCC 5 cut(s) 167, 249, 744, 797, 878
BspCNI CTCAG 1 cut(s) 460
BspFNI CGCG 5 cut(s) 215, 470, 544, 807, 824
BspLI GGNNCC 6 cut(s) 266, 348, 360, 497, 511, 755
BspMAI CTGCAG 2 cut(s) 414, 894
BspMI ACCTGC 1 cut(s) 203
BspPI GGATC 1 cut(s) 322
BspQI GCTCTTC 2 cut(s) 227, 302
BspT107I GGYRCC 1 cut(s) 358
BspTNI GGTCTC 1 cut(s) 246
BsrBI CCGCTC 1 cut(s) 127
BsrFI RCCGGY 3 cut(s) 86, 110, 267
BsrGI TGTACA 1 cut(s) 816
BsrI ACTGG 1 cut(s) 104
BssAI RCCGGY 3 cut(s) 86, 110, 267
BssECI CCNNGG 4 cut(s) 134, 213, 634, 879
BssHII GCGCGC 2 cut(s) 805, 822
BssMI GATC 4 cut(s) 314, 504, 894, 960
BssNI GRCGYC 2 cut(s) 231, 971
BssSI CACGAG 2 cut(s) 694, 836
Bst2BI CACGAG 2 cut(s) 694, 836
Bst2UI CCWGG 3 cut(s) 292, 635, 860
Bst4CI ACNGT 1 cut(s) 790
Bst6I CTCTTC 3 cut(s) 227, 302, 613
BstACI GRCGYC 2 cut(s) 231, 971
BstAPI GCANNNNNTGC 1 cut(s) 630
BstAUI TGTACA 1 cut(s) 816
BstBAI YACGTR 1 cut(s) 846
BstC8I GCNNGC 6 cut(s) 244, 460, 734, 807, 824, 876
BstDEI CTNAG 1 cut(s) 447
BstDSI CCRYGG 2 cut(s) 213, 879
BstEII GGTNACC 1 cut(s) 113
BstF5I GGATG 2 cut(s) 159, 626
BstFNI CGCG 5 cut(s) 215, 470, 544, 807, 824
BstH2I RGCGCY 1 cut(s) 642
BstHHI GCGC 5 cut(s) 641, 807, 809, 824, 826
BstKTI GATC 4 cut(s) 317, 507, 897, 963
BstMAI GTCTC 2 cut(s) 246, 978
BstMBI GATC 4 cut(s) 314, 504, 894, 960
BstMCI CGRYCG 4 cut(s) 250, 262, 789, 798
BstNI CCWGG 3 cut(s) 292, 635, 860
BstNSI RCATGY 2 cut(s) 246, 871
BstPI GGTNACC 1 cut(s) 113
BstSCI CCNGG 7 cut(s) 133, 162, 290, 633, 761, 852, 858
BstSFI CTRYAG 2 cut(s) 410, 890
BstSLI GKGCMC 1 cut(s) 363
BstUI CGCG 5 cut(s) 215, 470, 544, 807, 824
BstV1I GCAGC 6 cut(s) 260, 396, 449, 506, 789, 876
BstXI CCANNNNNNTGG 1 cut(s) 634
BstZI CGGCCG 2 cut(s) 247, 795
BsuI GTATCC 1 cut(s) 334
BsuRI GGCC 5 cut(s) 167, 249, 744, 797, 878
BtgI CCRYGG 2 cut(s) 213, 879
BtgZI GCGATG 1 cut(s) 906
BtrI CACGTC 1 cut(s) 750
BtsCI GGATG 2 cut(s) 159, 626
BtsIMutI CAGTG 1 cut(s) 111
BtuMI TCGCGA 1 cut(s) 544
BveI ACCTGC 1 cut(s) 203
Cac8I GCNNGC 6 cut(s) 244, 460, 734, 807, 824, 876
CfoI GCGC 5 cut(s) 641, 807, 809, 824, 826
Cfr10I RCCGGY 3 cut(s) 86, 110, 267
Cfr13I GGNCC 3 cut(s) 166, 742, 856
Cfr42I CCGCGG 1 cut(s) 216
CseI GACGC 4 cut(s) 220, 388, 476, 960
Csp6I GTAC 3 cut(s) 613, 778, 817
CspAI ACCGGT 1 cut(s) 110
CviAII CATG 2 cut(s) 243, 868
CviQI GTAC 3 cut(s) 613, 778, 817
DdeI CTNAG 1 cut(s) 447
DpnI GATC 4 cut(s) 316, 506, 896, 962
DpnII GATC 4 cut(s) 314, 504, 894, 960
DraIII CACNNNGTG 1 cut(s) 630
EaeI YGGCCR 3 cut(s) 247, 795, 876
EagI CGGCCG 2 cut(s) 247, 795
Eam1104I CTCTTC 3 cut(s) 227, 302, 613
EarI CTCTTC 3 cut(s) 227, 302, 613
Ecl136II GAGCTC 2 cut(s) 220, 328
EclXI CGGCCG 2 cut(s) 247, 795
Eco24I GRGCYC 2 cut(s) 222, 330
Eco31I GGTCTC 1 cut(s) 246
Eco47I GGWCC 1 cut(s) 856
Eco52I CGGCCG 2 cut(s) 247, 795
Eco53kI GAGCTC 2 cut(s) 220, 328
Eco72I CACGTG 1 cut(s) 846
Eco88I CYCGRG 2 cut(s) 323, 587
Eco91I GGTNACC 1 cut(s) 113
EcoICRI GAGCTC 2 cut(s) 220, 328
EcoO65I GGTNACC 1 cut(s) 113
EcoRII CCWGG 3 cut(s) 290, 633, 858
EcoT38I GRGCYC 2 cut(s) 222, 330
Esp3I CGTCTC 1 cut(s) 978
FaeI CATG 2 cut(s) 246, 871
FaiI YATR 4 cut(s) 75, 244, 812, 869
FaqI GGGAC 2 cut(s) 160, 698
FatI CATG 2 cut(s) 242, 867
FauI CCCGC 3 cut(s) 532, 673, 837
FbaI TGATCA 1 cut(s) 960
FblI GTMKAC 1 cut(s) 91
FokI GGATG 2 cut(s) 146, 633
FriOI GRGCYC 2 cut(s) 222, 330
GlaI GCGC 5 cut(s) 640, 806, 808, 823, 825
GsaI CCCAGC 1 cut(s) 124
GsuI CTGGAG 2 cut(s) 130, 629
HaeII RGCGCY 1 cut(s) 642
HaeIII GGCC 5 cut(s) 167, 249, 744, 797, 878
HgaI GACGC 4 cut(s) 220, 388, 476, 960
HhaI GCGC 5 cut(s) 641, 807, 809, 824, 826
Hin1I GRCGYC 2 cut(s) 231, 971
Hin1II CATG 2 cut(s) 246, 871
Hin6I GCGC 5 cut(s) 639, 805, 807, 822, 824
HinP1I GCGC 5 cut(s) 639, 805, 807, 822, 824
HincII GTYRAC 1 cut(s) 403
HindII GTYRAC 1 cut(s) 403
HinfI GANTC 6 cut(s) 386, 442, 489, 583, 884, 949
HphI GGTGA 1 cut(s) 107
Hpy166II GTNNAC 2 cut(s) 92, 403
Hpy188I TCNGA 3 cut(s) 340, 786, 960
Hpy188III TCNNGA 7 cut(s) 543, 580, 587, 589, 618, 646, 898
Hpy8I GTNNAC 2 cut(s) 92, 403
Hpy99I CGWCG 4 cut(s) 101, 340, 355, 470
HpyAV CCTTC 1 cut(s) 541
HpyCH4III ACNGT 1 cut(s) 790
HpyCH4IV ACGT 3 cut(s) 537, 749, 845
HpyCH4V TGCA 3 cut(s) 412, 624, 892
HpyF3I CTNAG 1 cut(s) 447
HpySE526I ACGT 3 cut(s) 537, 749, 845
Hsp92I GRCGYC 2 cut(s) 231, 971
Hsp92II CATG 2 cut(s) 246, 871
HspAI GCGC 5 cut(s) 639, 805, 807, 822, 824
Ksp22I TGATCA 1 cut(s) 960
KspI CCGCGG 1 cut(s) 216
Kzo9I GATC 4 cut(s) 314, 504, 894, 960
LguI GCTCTTC 2 cut(s) 227, 302
LmnI GCTCC 9 cut(s) 217, 264, 333, 352, 501, 515, 663, 690, 759
Lsp1109I GCAGC 6 cut(s) 260, 396, 449, 506, 789, 876
LweI GCATC 3 cut(s) 366, 611, 929
MaeII ACGT 3 cut(s) 537, 749, 845
MaeIII GTNAC 3 cut(s) 106, 113, 942
MalI GATC 4 cut(s) 316, 506, 896, 962
MbiI CCGCTC 1 cut(s) 127
MboI GATC 4 cut(s) 314, 504, 894, 960
MboII GAAGA 6 cut(s) 5, 214, 289, 600, 604, 928
MhlI GDGCHC 4 cut(s) 222, 330, 363, 695
MlyI GAGTC 1 cut(s) 380
MmeI TCCRAC 2 cut(s) 318, 809
MseI TTAA 1 cut(s) 59
MspA1I CMGCKG 4 cut(s) 215, 462, 575, 874
MspR9I CCNGG 7 cut(s) 135, 164, 292, 635, 763, 854, 860
MvaI CCWGG 3 cut(s) 292, 635, 860
MvnI CGCG 5 cut(s) 215, 470, 544, 807, 824
NciI CCSGG 4 cut(s) 135, 164, 763, 854
NdeII GATC 4 cut(s) 314, 504, 894, 960
NlaIII CATG 2 cut(s) 246, 871
NlaIV GGNNCC 6 cut(s) 266, 348, 360, 497, 511, 755
NmeAIII GCCGAG 1 cut(s) 275
NmuCI GTSAC 2 cut(s) 106, 113
NruI TCGCGA 1 cut(s) 544
NspI RCATGY 2 cut(s) 246, 871
PaeI GCATGC 1 cut(s) 246
PaeR7I CTCGAG 2 cut(s) 323, 587
PauI GCGCGC 2 cut(s) 805, 822
PciSI GCTCTTC 2 cut(s) 227, 302
PcsI WCGNNNNNNNCGW 1 cut(s) 462
PfeI GAWTC 5 cut(s) 442, 489, 583, 884, 949
PflMI CCANNNNNTGG 1 cut(s) 739
PinAI ACCGGT 1 cut(s) 110
PleI GAGTC 1 cut(s) 380
PmaCI CACGTG 1 cut(s) 846
PmlI CACGTG 1 cut(s) 846
PpsI GAGTC 1 cut(s) 380
Ppu21I YACGTR 1 cut(s) 846
PshBI ATTAAT 1 cut(s) 59
Psp124BI GAGCTC 2 cut(s) 222, 330
Psp6I CCWGG 3 cut(s) 290, 633, 858
PspCI CACGTG 1 cut(s) 846
PspEI GGTNACC 1 cut(s) 113
PspFI CCCAGC 1 cut(s) 120
PspGI CCWGG 3 cut(s) 290, 633, 858
PspN4I GGNNCC 6 cut(s) 266, 348, 360, 497, 511, 755
PspPI GGNCC 3 cut(s) 166, 742, 856
PspXI VCTCGAGB 1 cut(s) 323
PstI CTGCAG 2 cut(s) 414, 894
PteI GCGCGC 2 cut(s) 805, 822
PvuII CAGCTG 1 cut(s) 462
RruI TCGCGA 1 cut(s) 544
RsaI GTAC 3 cut(s) 614, 779, 818
RsaNI GTAC 3 cut(s) 613, 778, 817
SacI GAGCTC 2 cut(s) 222, 330
SacII CCGCGG 1 cut(s) 216
SapI GCTCTTC 2 cut(s) 227, 302
SaqAI TTAA 1 cut(s) 59
Sau3AI GATC 4 cut(s) 314, 504, 894, 960
Sau96I GGNCC 3 cut(s) 166, 742, 856
SchI GAGTC 1 cut(s) 380
ScrFI CCNGG 7 cut(s) 135, 164, 292, 635, 763, 854, 860
SduI GDGCHC 4 cut(s) 222, 330, 363, 695
SfaNI GCATC 3 cut(s) 366, 611, 929
SfcI CTRYAG 2 cut(s) 410, 890
Sfr274I CTCGAG 2 cut(s) 323, 587
Sfr303I CCGCGG 1 cut(s) 216
SgrBI CCGCGG 1 cut(s) 216
SinI GGWCC 1 cut(s) 856
SlaI CTCGAG 2 cut(s) 323, 587
SmlI CTYRAG 4 cut(s) 323, 514, 587, 653
SmoI CTYRAG 4 cut(s) 323, 514, 587, 653
SphI GCATGC 1 cut(s) 246
SstI GAGCTC 2 cut(s) 222, 330
StyD4I CCNGG 7 cut(s) 133, 162, 290, 633, 761, 852, 858
TaaI ACNGT 1 cut(s) 790
TaiI ACGT 3 cut(s) 540, 752, 848
TaqI TCGA 6 cut(s) 99, 204, 258, 324, 588, 897
TatI WGTACW 1 cut(s) 816
TfiI GAWTC 5 cut(s) 442, 489, 583, 884, 949
Tru1I TTAA 1 cut(s) 59
Tru9I TTAA 1 cut(s) 59
TscAI CASTG 1 cut(s) 111
TseFI GTSAC 2 cut(s) 106, 113
TseI GCWGC 6 cut(s) 273, 409, 462, 519, 802, 889
Tsp45I GTSAC 2 cut(s) 106, 113
TspDTI ATGAA 1 cut(s) 62
TspGWI ACGGA 2 cut(s) 339, 687
TspRI CASTG 1 cut(s) 111
Van91I CCANNNNNTGG 1 cut(s) 739
VpaK11BI GGWCC 1 cut(s) 856
VspI ATTAAT 1 cut(s) 59
XceI RCATGY 2 cut(s) 246, 871
XhoI CTCGAG 2 cut(s) 323, 587
XmiI GTMKAC 1 cut(s) 91
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.