pycom17g08750

Belongs to the NPH3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0004787
Physical Location & Seq
Reverse (-)
6599443 .. 6600605
1163 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1020 bp
ATGCTTGTCAAGGCCATGGTTTCACGAAAACTCGATCATGTTATCATTAGTAGGTTCCTCTTTTATTACCAGAAATCGAAGTTTTACACGGTCAAATCTGATACCAAGCGCACCATTGCTGAAACGGTCATTGAGATGCTGTTTATGCTTGATCAGAGCTGTGTTTCTTGCAAGAGCTTATTCGGGATTCTACATGTTTCGCTGAATTTTAACTTAAACAGAAGTATCAGGAATAAGTTGGAGAATATGATTGGTTCACAACTAGATAAAGCAACATTGGACAATTTGCTTGTTCCATCCCCACAAGGAATCAATTACTTGTATGATGTCAATCTTGTTCTAAGGTTCTTGAAATCATTTCTGCGTGATGGAGGAACCTGTCAAGCGTCTCCAATCCGATTAAGGAAAGTTTCTGGCTTGGTTGATTTGTATATAGCAGAAGTAGCCCCAGATCCTTGTTTGAAGCATTCAAAGTTTCTGGCTTTAGCTATCGCCCTGCCGGATTCGGCAAGAGACTCCTATGATGAGCTCTACCATGCCATAGACATGTATATACAGGTGCACGCAGGATTGTCTGAAGACAAAAAGATGAAAATATGTTGTGTGCTGAACTATGAGAAGCTCTCGGCAGAGACTTGTATACACCTTTCAAAGAACACAAAATTTCCATCAAAATCTGCAGTCCAAGCTCTTGTCTCTCAGCAATCCAAGCTCAAAAAGTTACTCCAAACAACCAACAACTTTAATTCCTATGCCACCTCCCCTTATAGCGCCACCACTGAGGCAAAAAATTGGGGACTAAGGAAAGATGATGCCAACGAACAAAATGTGCTTTACGCCGGAAAACTCGATCTCTCAACTGACAAGGTGAAGCTCAGAGCACATTTGCAAGGCATGCAATGCAGGGTCATGGAATTGGAGAAAGTTTGCAAGAAAATGCAATCTCAAATGGCAAAGTTTACAAAATCGAAAGCATCAAGCCACAGCCATACAAGATCATTGCCCAAACTTTGTTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BTB NPH3

Protein Analysis

340

Amino Acids

38.34

Weight (kDa)

9.45

Isoelectric Point (pI)

40.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NPH3 PF03000 2 - 213 3.7e-55 NPH3 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 640
AclWI GGATC 1 cut(s) 446
AcsI RAATTY 2 cut(s) 205, 662
AcuI CTGAAG 1 cut(s) 597
AflIII ACRYGT 2 cut(s) 193, 546
AgsI TTSAA 4 cut(s) 352, 463, 471, 651
AluBI AGCT 8 cut(s) 159, 177, 488, 529, 622, 689, 712, 874
AluI AGCT 8 cut(s) 159, 177, 488, 529, 622, 689, 712, 874
Alw21I GWGCWC 3 cut(s) 531, 564, 883
Alw26I GTCTC 4 cut(s) 393, 507, 626, 700
Alw44I GTGCAC 1 cut(s) 560
AlwI GGATC 1 cut(s) 446
AoxI GGCC 1 cut(s) 12
ApaLI GTGCAC 1 cut(s) 560
ApoI RAATTY 2 cut(s) 205, 662
AspLEI GCGC 2 cut(s) 111, 773
AsuHPI GGTGA 1 cut(s) 880
BaeGI GKGCMC 1 cut(s) 564
BaeI ACNNNNGTAYC 2 cut(s) 208, 241
BanII GRGCYC 1 cut(s) 531
BbsI GAAGAC 1 cut(s) 585
Bbv12I GWGCWC 3 cut(s) 531, 564, 883
BccI CCATC 3 cut(s) 304, 362, 676
BclI TGATCA 1 cut(s) 151
BcoDI GTCTC 4 cut(s) 393, 507, 626, 700
BfaI CTAG 1 cut(s) 263
BfmI CTRYAG 1 cut(s) 678
BfoI RGCGCY 1 cut(s) 774
BmiI GGNNCC 2 cut(s) 56, 376
BmsI GCATC 3 cut(s) 126, 802, 983
BpiI GAAGAC 1 cut(s) 585
BplI GAGNNNNNCTC 2 cut(s) 608, 640
BsaBI GATNNNNATC 1 cut(s) 330
BsaJI CCNNGG 1 cut(s) 15
Bse3DI GCAATG 3 cut(s) 114, 905, 998
Bse8I GATNNNNATC 1 cut(s) 330
BseDI CCNNGG 1 cut(s) 15
BseGI GGATG 1 cut(s) 296
BseJI GATNNNNATC 1 cut(s) 330
BseMI GCAATG 3 cut(s) 114, 905, 998
BseMII CTCAG 3 cut(s) 713, 771, 889
BseSI GKGCMC 1 cut(s) 564
BshFI GGCC 1 cut(s) 14
BsiHKAI GWGCWC 3 cut(s) 531, 564, 883
BsiSI CCGG 2 cut(s) 500, 840
BslFI GGGAC 1 cut(s) 810
BsmAI GTCTC 4 cut(s) 393, 507, 626, 700
BsmBI CGTCTC 1 cut(s) 393
BsmFI GGGAC 1 cut(s) 810
BsmI GAATGC 1 cut(s) 466
BsnI GGCC 1 cut(s) 14
Bsp1286I GDGCHC 3 cut(s) 531, 564, 883
Bsp143I GATC 5 cut(s) 34, 151, 451, 850, 995
Bsp19I CCATGG 1 cut(s) 15
BspANI GGCC 1 cut(s) 14
BspCNI CTCAG 3 cut(s) 712, 772, 888
BspHI TCATGA 1 cut(s) 1016
BspLI GGNNCC 2 cut(s) 56, 376
BspMAI CTGCAG 1 cut(s) 682
BspPI GGATC 1 cut(s) 446
BsrDI GCAATG 3 cut(s) 114, 905, 998
BssECI CCNNGG 1 cut(s) 15
BssMI GATC 5 cut(s) 34, 151, 451, 850, 995
BssNAI GTATAC 1 cut(s) 641
BssT1I CCWWGG 1 cut(s) 15
Bst1107I GTATAC 1 cut(s) 641
Bst4CI ACNGT 2 cut(s) 91, 127
BstAPI GCANNNNNTGC 2 cut(s) 895, 900
BstC8I GCNNGC 2 cut(s) 564, 896
BstDEI CTNAG 5 cut(s) 341, 699, 780, 800, 875
BstDSI CCRYGG 1 cut(s) 15
BstF5I GGATG 1 cut(s) 296
BstH2I RGCGCY 1 cut(s) 774
BstHHI GCGC 2 cut(s) 111, 773
BstKTI GATC 5 cut(s) 37, 154, 454, 853, 998
BstMAI GTCTC 4 cut(s) 393, 507, 626, 700
BstMBI GATC 5 cut(s) 34, 151, 451, 850, 995
BstMWI GCNNNNNNNGC 6 cut(s) 145, 443, 686, 709, 895, 900
BstNSI RCATGY 3 cut(s) 197, 550, 898
BstSFI CTRYAG 1 cut(s) 678
BstSLI GKGCMC 1 cut(s) 564
BstV2I GAAGAC 1 cut(s) 585
BstX2I RGATCY 1 cut(s) 451
BstYI RGATCY 1 cut(s) 451
BstZ17I GTATAC 1 cut(s) 641
BsuRI GGCC 1 cut(s) 14
BtgI CCRYGG 1 cut(s) 15
BtsCI GGATG 1 cut(s) 296
BtsIMutI CAGTG 1 cut(s) 777
Cac8I GCNNGC 2 cut(s) 564, 896
CciI TCATGA 1 cut(s) 1016
CfoI GCGC 2 cut(s) 111, 773
CseI GACGC 1 cut(s) 375
CviAII CATG 8 cut(s) 16, 38, 194, 536, 547, 895, 910, 1017
DdeI CTNAG 5 cut(s) 341, 699, 780, 800, 875
DpnI GATC 5 cut(s) 36, 153, 453, 852, 997
DpnII GATC 5 cut(s) 34, 151, 451, 850, 995
Ecl136II GAGCTC 1 cut(s) 529
Eco130I CCWWGG 1 cut(s) 15
Eco24I GRGCYC 1 cut(s) 531
Eco53kI GAGCTC 1 cut(s) 529
Eco57I CTGAAG 1 cut(s) 597
EcoICRI GAGCTC 1 cut(s) 529
EcoT14I CCWWGG 1 cut(s) 15
EcoT38I GRGCYC 1 cut(s) 531
ErhI CCWWGG 1 cut(s) 15
Esp3I CGTCTC 1 cut(s) 393
FaeI CATG 8 cut(s) 19, 41, 197, 539, 550, 898, 913, 1020
FaqI GGGAC 1 cut(s) 810
FatI CATG 8 cut(s) 15, 37, 193, 535, 546, 894, 909, 1016
FbaI TGATCA 1 cut(s) 151
FblI GTMKAC 1 cut(s) 640
FokI GGATG 1 cut(s) 283
FriOI GRGCYC 1 cut(s) 531
FspBI CTAG 1 cut(s) 263
GlaI GCGC 2 cut(s) 110, 772
HaeII RGCGCY 1 cut(s) 774
HaeIII GGCC 1 cut(s) 14
HapII CCGG 2 cut(s) 500, 840
HgaI GACGC 1 cut(s) 375
HhaI GCGC 2 cut(s) 111, 773
Hin1II CATG 8 cut(s) 19, 41, 197, 539, 550, 898, 913, 1020
Hin6I GCGC 2 cut(s) 109, 771
HinP1I GCGC 2 cut(s) 109, 771
HinfI GANTC 4 cut(s) 187, 309, 503, 515
HpaII CCGG 2 cut(s) 500, 840
HphI GGTGA 1 cut(s) 880
Hpy166II GTNNAC 4 cut(s) 257, 562, 641, 960
Hpy188I TCNGA 5 cut(s) 100, 156, 398, 577, 878
Hpy188III TCNNGA 5 cut(s) 24, 184, 229, 349, 1017
Hpy8I GTNNAC 4 cut(s) 257, 562, 641, 960
HpyCH4III ACNGT 2 cut(s) 91, 127
HpyCH4V TGCA 8 cut(s) 171, 562, 680, 889, 898, 903, 930, 940
HpyF10VI GCNNNNNNNGC 6 cut(s) 145, 443, 686, 709, 895, 900
HpyF3I CTNAG 5 cut(s) 341, 699, 780, 800, 875
Hsp92II CATG 8 cut(s) 19, 41, 197, 539, 550, 898, 913, 1020
HspAI GCGC 2 cut(s) 109, 771
Ksp22I TGATCA 1 cut(s) 151
Kzo9I GATC 5 cut(s) 34, 151, 451, 850, 995
LweI GCATC 3 cut(s) 126, 802, 983
MaeI CTAG 1 cut(s) 263
MaeIII GTNAC 1 cut(s) 720
MalI GATC 5 cut(s) 36, 153, 453, 852, 997
MboI GATC 5 cut(s) 34, 151, 451, 850, 995
MboII GAAGA 1 cut(s) 590
MflI RGATCY 1 cut(s) 451
MhlI GDGCHC 3 cut(s) 531, 564, 883
MluCI AATT 7 cut(s) 205, 283, 313, 662, 745, 790, 914
MlyI GAGTC 1 cut(s) 509
MmeI TCCRAC 1 cut(s) 219
MnlI CCTC 4 cut(s) 68, 365, 769, 775
MseI TTAA 4 cut(s) 210, 215, 401, 744
MslI CAYNNNNRTG 2 cut(s) 134, 545
MspI CCGG 2 cut(s) 500, 840
Mva1269I GAATGC 1 cut(s) 466
MwoI GCNNNNNNNGC 6 cut(s) 145, 443, 686, 709, 895, 900
NcoI CCATGG 1 cut(s) 15
NdeII GATC 5 cut(s) 34, 151, 451, 850, 995
NlaIII CATG 8 cut(s) 19, 41, 197, 539, 550, 898, 913, 1020
NlaIV GGNNCC 2 cut(s) 56, 376
NmeAIII GCCGAG 1 cut(s) 605
NspI RCATGY 3 cut(s) 197, 550, 898
PaeI GCATGC 1 cut(s) 898
PagI TCATGA 1 cut(s) 1016
PciI ACATGT 2 cut(s) 193, 546
PctI GAATGC 1 cut(s) 466
PfeI GAWTC 3 cut(s) 187, 309, 503
PleI GAGTC 1 cut(s) 509
PpsI GAGTC 1 cut(s) 509
PscI ACATGT 2 cut(s) 193, 546
Psp124BI GAGCTC 1 cut(s) 531
PspN4I GGNNCC 2 cut(s) 56, 376
PstI CTGCAG 1 cut(s) 682
PsuI RGATCY 1 cut(s) 451
RseI CAYNNNNRTG 2 cut(s) 134, 545
SacI GAGCTC 1 cut(s) 531
SaqAI TTAA 4 cut(s) 210, 215, 401, 744
Sau3AI GATC 5 cut(s) 34, 151, 451, 850, 995
SchI GAGTC 1 cut(s) 509
SduI GDGCHC 3 cut(s) 531, 564, 883
SfaNI GCATC 3 cut(s) 126, 802, 983
SfcI CTRYAG 1 cut(s) 678
SmiMI CAYNNNNRTG 2 cut(s) 134, 545
SphI GCATGC 1 cut(s) 898
Sse9I AATT 7 cut(s) 205, 283, 313, 662, 745, 790, 914
SspMI CTAG 1 cut(s) 263
SstI GAGCTC 1 cut(s) 531
StyI CCWWGG 1 cut(s) 15
TaaI ACNGT 2 cut(s) 91, 127
TaqI TCGA 4 cut(s) 33, 77, 849, 968
TasI AATT 7 cut(s) 205, 283, 313, 662, 745, 790, 914
TfiI GAWTC 3 cut(s) 187, 309, 503
Tru1I TTAA 4 cut(s) 210, 215, 401, 744
Tru9I TTAA 4 cut(s) 210, 215, 401, 744
TscAI CASTG 1 cut(s) 784
TspDTI ATGAA 2 cut(s) 605, 1005
TspRI CASTG 1 cut(s) 784
VneI GTGCAC 1 cut(s) 560
XapI RAATTY 2 cut(s) 205, 662
XceI RCATGY 3 cut(s) 197, 550, 898
XmiI GTMKAC 1 cut(s) 640
XspI CTAG 1 cut(s) 263
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.