pycom17g08880

Beta-amylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0006751
Physical Location & Seq
Reverse (-)
6693868 .. 6694749
882 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 426 bp
ATGTTGTCAAGGCATCATGCAATTTTGAATTTCACATGTTTAGAGATGAGGAACTCAGAACAAAGTGCTGATGCCAAAAGTGCGCCTCAGGAACTTGTTCAGCAGGTTTTGAGTGGAGGTTGGAGAGAAAACATAGAAGTTGCTGGAGAAAATGCACTTTCTAGATACGACAGTGTGGCTTATGACCAAATCCTTCTAAATGCTAGGCCAAATGGCATCAATAAAGACGGCCAACCAAAATTAAGGATGTACGGGGTGACATACCTTCGTTTGTCTGATGAACTACTACAAAAGACGAATCTTGATACGTTCAAGATATTTGTAAAAAAGATGCATGCTGATGAGGCAAGATTACCGTCCAGACCCAGAGAAGTACAATCACCACCTAGGTCCTTTGAAGCGGTCGAAACCAGAGACTTCGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 map00500
Pfam Domains
Protein Families
Glyco_hydro_14

Protein Analysis

142

Amino Acids

16.12

Weight (kDa)

6.31

Isoelectric Point (pI)

64.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_14 PF01373 2 - 96 2.1e-13 Glycosyl hydrolase family 14
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 94
AciI CCGC 1 cut(s) 401
AcoI YGGCCR 1 cut(s) 229
AcsI RAATTY 1 cut(s) 28
AfaI GTAC 2 cut(s) 251, 375
AflIII ACRYGT 1 cut(s) 35
AgsI TTSAA 3 cut(s) 28, 313, 398
Alw26I GTCTC 1 cut(s) 408
AoxI GGCC 2 cut(s) 206, 229
ApoI RAATTY 1 cut(s) 28
Asp700I GAANNNNTTC 1 cut(s) 96
AspA2I CCTAGG 1 cut(s) 386
AspLEI GCGC 1 cut(s) 85
AspS9I GGNCC 1 cut(s) 390
AsuHPI GGTGA 2 cut(s) 268, 372
AvaII GGWCC 1 cut(s) 390
AvrII CCTAGG 1 cut(s) 386
AxyI CCTNAGG 1 cut(s) 87
BaeI ACNNNNGTAYC 2 cut(s) 157, 190
BceAI ACGGC 1 cut(s) 244
BcoDI GTCTC 1 cut(s) 408
BfaI CTAG 3 cut(s) 162, 204, 387
BfuAI ACCTGC 1 cut(s) 94
BlnI CCTAGG 1 cut(s) 386
Bme18I GGWCC 1 cut(s) 390
BmgT120I GGNCC 1 cut(s) 390
BmsI GCATC 4 cut(s) 22, 61, 225, 321
BpmI CTGGAG 1 cut(s) 165
BsaJI CCNNGG 1 cut(s) 386
Bse21I CCTNAGG 1 cut(s) 87
BseDI CCNNGG 1 cut(s) 386
BseGI GGATG 1 cut(s) 252
BseMII CTCAG 2 cut(s) 69, 101
Bsh1285I CGRYCG 1 cut(s) 405
BshFI GGCC 2 cut(s) 208, 231
BsiEI CGRYCG 1 cut(s) 405
BsmAI GTCTC 1 cut(s) 408
BsnI GGCC 2 cut(s) 208, 231
BspACI CCGC 1 cut(s) 401
BspANI GGCC 2 cut(s) 208, 231
BspCNI CTCAG 2 cut(s) 68, 100
BspMI ACCTGC 1 cut(s) 94
BssECI CCNNGG 1 cut(s) 386
BssT1I CCWWGG 1 cut(s) 386
Bst4CI ACNGT 2 cut(s) 173, 357
BstC8I GCNNGC 1 cut(s) 336
BstDEI CTNAG 2 cut(s) 55, 87
BstF5I GGATG 1 cut(s) 252
BstHHI GCGC 1 cut(s) 85
BstMAI GTCTC 1 cut(s) 408
BstMCI CGRYCG 1 cut(s) 405
BstMWI GCNNNNNNNGC 2 cut(s) 80, 344
BstNSI RCATGY 2 cut(s) 39, 338
Bsu36I CCTNAGG 1 cut(s) 87
BsuRI GGCC 2 cut(s) 208, 231
BtsCI GGATG 1 cut(s) 252
BtsIMutI CAGTG 1 cut(s) 178
BveI ACCTGC 1 cut(s) 94
Cac8I GCNNGC 1 cut(s) 336
CfoI GCGC 1 cut(s) 85
Cfr13I GGNCC 1 cut(s) 390
Csp6I GTAC 2 cut(s) 250, 374
CviAII CATG 3 cut(s) 17, 36, 335
CviJI RGCY 3 cut(s) 179, 208, 231
CviKI_1 RGCY 3 cut(s) 179, 208, 231
CviQI GTAC 2 cut(s) 250, 374
DdeI CTNAG 2 cut(s) 55, 87
EaeI YGGCCR 1 cut(s) 229
Eco130I CCWWGG 1 cut(s) 386
Eco47I GGWCC 1 cut(s) 390
Eco81I CCTNAGG 1 cut(s) 87
EcoO109I RGGNCCY 1 cut(s) 390
EcoT14I CCWWGG 1 cut(s) 386
EcoT22I ATGCAT 1 cut(s) 336
ErhI CCWWGG 1 cut(s) 386
FaeI CATG 3 cut(s) 20, 39, 338
FaiI YATR 6 cut(s) 18, 37, 134, 183, 262, 336
FatI CATG 3 cut(s) 16, 35, 334
FokI GGATG 1 cut(s) 259
FspBI CTAG 3 cut(s) 162, 204, 387
GlaI GCGC 1 cut(s) 84
GsuI CTGGAG 1 cut(s) 165
HaeIII GGCC 2 cut(s) 208, 231
HhaI GCGC 1 cut(s) 85
Hin1II CATG 3 cut(s) 20, 39, 338
Hin6I GCGC 1 cut(s) 83
HinP1I GCGC 1 cut(s) 83
HinfI GANTC 1 cut(s) 298
HphI GGTGA 2 cut(s) 268, 372
Hpy188I TCNGA 2 cut(s) 58, 277
Hpy188III TCNNGA 5 cut(s) 89, 162, 302, 313, 360
HpyAV CCTTC 2 cut(s) 203, 275
HpyCH4III ACNGT 2 cut(s) 173, 357
HpyCH4IV ACGT 1 cut(s) 308
HpyCH4V TGCA 3 cut(s) 20, 155, 334
HpyF10VI GCNNNNNNNGC 2 cut(s) 80, 344
HpyF3I CTNAG 2 cut(s) 55, 87
HpySE526I ACGT 1 cut(s) 308
Hsp92II CATG 3 cut(s) 20, 39, 338
HspAI GCGC 1 cut(s) 83
LpnPI CCDG 5 cut(s) 74, 89, 129, 373, 379
LweI GCATC 4 cut(s) 22, 61, 225, 321
MaeI CTAG 3 cut(s) 162, 204, 387
MaeII ACGT 1 cut(s) 308
MaeIII GTNAC 1 cut(s) 256
MluCI AATT 3 cut(s) 21, 28, 239
MmeI TCCRAC 1 cut(s) 101
MnlI CCTC 4 cut(s) 42, 96, 110, 337
Mph1103I ATGCAT 1 cut(s) 336
MroXI GAANNNNTTC 1 cut(s) 96
MseI TTAA 1 cut(s) 242
MslI CAYNNNNRTG 1 cut(s) 339
MwoI GCNNNNNNNGC 2 cut(s) 80, 344
NlaIII CATG 3 cut(s) 20, 39, 338
NmuCI GTSAC 1 cut(s) 256
NsiI ATGCAT 1 cut(s) 336
NspI RCATGY 2 cut(s) 39, 338
PaeI GCATGC 1 cut(s) 338
PciI ACATGT 1 cut(s) 35
PdmI GAANNNNTTC 1 cut(s) 96
PfeI GAWTC 1 cut(s) 298
PpuMI RGGWCCY 1 cut(s) 390
PscI ACATGT 1 cut(s) 35
Psp5II RGGWCCY 1 cut(s) 390
PspPI GGNCC 1 cut(s) 390
PspPPI RGGWCCY 1 cut(s) 390
RsaI GTAC 2 cut(s) 251, 375
RsaNI GTAC 2 cut(s) 250, 374
RseI CAYNNNNRTG 1 cut(s) 339
SaqAI TTAA 1 cut(s) 242
Sau96I GGNCC 1 cut(s) 390
SetI ASST 6 cut(s) 108, 121, 267, 311, 388, 392
SfaNI GCATC 4 cut(s) 22, 61, 225, 321
SinI GGWCC 1 cut(s) 390
SmiMI CAYNNNNRTG 1 cut(s) 339
SphI GCATGC 1 cut(s) 338
Sse9I AATT 3 cut(s) 21, 28, 239
SsiI CCGC 1 cut(s) 401
SspMI CTAG 3 cut(s) 162, 204, 387
StyI CCWWGG 1 cut(s) 386
TaaI ACNGT 2 cut(s) 173, 357
TaiI ACGT 1 cut(s) 311
TaqI TCGA 2 cut(s) 405, 420
TasI AATT 3 cut(s) 21, 28, 239
TatI WGTACW 1 cut(s) 373
TfiI GAWTC 1 cut(s) 298
Tru1I TTAA 1 cut(s) 242
Tru9I TTAA 1 cut(s) 242
TscAI CASTG 1 cut(s) 178
TseFI GTSAC 1 cut(s) 256
Tsp45I GTSAC 1 cut(s) 256
TspDTI ATGAA 1 cut(s) 294
TspRI CASTG 1 cut(s) 178
VpaK11BI GGWCC 1 cut(s) 390
XapI RAATTY 1 cut(s) 28
XbaI TCTAGA 1 cut(s) 161
XceI RCATGY 2 cut(s) 39, 338
XmaJI CCTAGG 1 cut(s) 386
XmnI GAANNNNTTC 1 cut(s) 96
XspI CTAG 3 cut(s) 162, 204, 387
Zsp2I ATGCAT 1 cut(s) 336
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.