pycom17g10460

Histidine kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0003045
Physical Location & Seq
Reverse (-)
8044171 .. 8048810
4640 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2373 bp
ATGTATCTGAATTGTAGGGTATTCTCTGGTTTTAATGGCAGATTGCCCTTCAATCTTGACTTGAATAAGGCAATGGAGGCTTTGTATGGGACTAGCTGTACTAGAAGATGGAGAGGAAAGGTTCTAATTCTGGGGCTTGTGGGTTTTGTTACAATCATTTGGGGTTTCATGAGTTTCAATGATGGAAGTTTGGGGATGAGAGAGAAAGCTCCAGATATGGGTGGACAGAAAGCTTGGATCCTGCAGCAGCATTTCAATATCAGCAAGTCCCAGGTTCTCGCTTTCGCTTCGTTGTTCTCCGAATCAGATCAGATAGCATCCCTTGAATGTCACAAAGAACCAGGACCGCGAATGATGTTAAGTAATGTCATTTCCTGTTCTCTGAAGGTGCTATGTTCAGATGAGGAGGCAGAAAATGAAGAAGCCAAGGACCAATGCCTGGTCCAAGATGAGAACATGCCCAAGAAGCGTGATGTATCATTGCTTGAGGACAAATCCGTATCATGCATCACGCAATCTACAGTTTCAGCAAATAGGATTTTTGGAAAGGATTGTGCAAAAGAGGACTCGCAGTTGCTTTGTACATTGGTAATACGATGGTGGTGGGCTTTTGTTGGAATGATCATCTGCTACAAACTGTCTGGTTTTTCTATGAAATTGTGGAGGAGTGAAAAACAGAAGGTGGTTCAAAAGGTTAAAGAGCAGCCATTGCCTCGGAGGAAGCAGCCAGAGAAACTGCAACAGCAAGCCCAAAAGCCTCCTAAGGTTGCTGGCACGTGGAGGACGAATCTACTTATAGTATTTATCATATTTGGCTGCATTACATCATTGTGGTTACTGTGTCACTTACGTGCAAGAGATCATTTGTGGAGGGAAGAAACACTTTCCAACATGTGCGACGAACGAGCCCGGATGTTGCAGGATCAGTTTAATGTGAGCCTGAATCATGTTCATGCTTTAGCTATTCTTGTATCCACCTTTCACCATGGGAAACAACCTACGGCAATTGATCAGAAAACATTTGGTGAATATACAGAGAGAACATCTTTTGAGAGACCCCTTACGAGTGGTGTTGCTTATGCTTTGAAAGTTACTCATGCTGAGAGGGAACATTTTGAGAAGGAACATGGATGGACAATAAAAAAAATGGAAACTGAGGACCAGACTCTAGTCCAAGATTTTATTACAGAAAGCTTGGCCTCTGCGCCTATTCAAGATGAATATGCGCCCGTGATCTTTTCTCAAGAAACTGTCTCCCACATTGTCTCTATTGACATGATGTCTGGAAAGGAAGACCGTGAGAACATCTTGCGAGCAAGGGCAACTGGAAAGGGAGTACTGACATCCCCGTTTAAGCTATTGAAATCCAATCACCTGGGAGTTGTACTCACATTCGCAGTCTATAATGCTGATTTGCCTCCAGATGCCACACCAGAGCAACGTATTCAAGCTACTGTGGGGTATCTAGGTGCATCTTACGATGTTCCATCGCTGGTGGAGAAGCTCTTGCACCAACTTGCCAGCAAGCAAACAATTGTTGTGAATGTTTATGATACAACTAATGCATCTGCTCCGATCAACATGTATGGTACTGATGTTGTTGATACTGGCCTGTTGGAAATTAGCAACCTTGATTTTGGGGATCCACAACGCAAACATGAAATGCACTGCAGGTTTAAGCACAAACCTCCGTTTCCTTTGACAGCAGCAAGTGCATCAATAGGAGTCCTAGTCATTACCTTGCTAGTTAGTCATATTTTTTATGCGGCCATAAGCCGAATAGCGAAAGTGGAGGCAGACTATCGTGAGATGATGGAACTCAAAGTTCGTGCAGAAGCTGCTGATGTGGCCAAATCTCAGTTCCTTGCAACTGTTTCCCATGAGATCAGGACACCAATGAATGGTGTTTTAGGCATGCTGCAAATGCTGATGGATACCGATCTAGGTCCAAACCAACAGGACTATGCTGAGACTGCTCATGCTAGCGGGAGAGAATTGATATCGCTGATAAATGAGGTTCTTGATCAGGCAAAAATTGAATCAGGCAGGCTGGAACTTGAAACCGTACCTTTTGATTTGCGTTCAGTGCTTGATAATGTTCTGTCACTTAACTCAGGAAAATCTAATGAGAAAGGAATTGAGTTGGCTGCTTATGTTTCCAATATGGTACCTGAAGTTGTCATTGGTGATCCTGGACGCTTTCGGCAGATAATTACAAATCTTGTTGGAAATTCAATTAAGTTCACCCATGACAAAGGACACATATTTGTCTCAGTGCATCTGGTGGATGAAGTGAGGGCCCCACCTTCAAAGAGATCTTATGGACGAAGTTCTAAGACAAGGCTTGAGCTTAGTTGGAGATGTCTCAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004673 GO:0004888 GO:0005488 GO:0005515 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009266 GO:0009409 GO:0009414 GO:0009415 GO:0009628 GO:0009636 GO:0009651 GO:0009719 GO:0009725 GO:0009735 GO:0009736 GO:0009737 GO:0009755 GO:0009884 GO:0009888 GO:0009894 GO:0009909 GO:0009987 GO:0010029 GO:0010033 GO:0010035 GO:0010087 GO:0010271 GO:0010959 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0016775 GO:0018106 GO:0018193 GO:0018202 GO:0019222 GO:0019538 GO:0019899 GO:0019900 GO:0019901 GO:0023052 GO:0031323 GO:0031329 GO:0032502 GO:0032870 GO:0032879 GO:0033554 GO:0033993 GO:0034756 GO:0034757 GO:0036211 GO:0038023 GO:0040007 GO:0042221 GO:0042802 GO:0043170 GO:0043269 GO:0043271 GO:0043412 GO:0043424 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0048509 GO:0048519 GO:0048580 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051049 GO:0051051 GO:0051171 GO:0051193 GO:0051239 GO:0051716 GO:0060089 GO:0065007 GO:0070417 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071368 GO:0071396 GO:0071495 GO:0071704 GO:0071944 GO:0080117 GO:0080190 GO:0090056 GO:0097305 GO:0097306 GO:0140096 GO:1900140 GO:1901401 GO:1901404 GO:1901564 GO:1901700 GO:1901701 GO:2000026 GO:2000241
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
CHASE HATPase_c HisKA Response_reg

Protein Analysis

791

Amino Acids

88.87

Weight (kDa)

7.09

Isoelectric Point (pI)

43.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CHASE PF03924 335 - 529 1.3e-36 CHASE domain
HisKA PF00512 617 - 682 4.5e-20 His Kinase A (phospho-acceptor) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1662
Acc65I GGTACC 1 cut(s) 2167
AccB1I GGYRCC 1 cut(s) 2167
AccB7I CCANNNNNTGG 2 cut(s) 439, 1901
AccII CGCG 1 cut(s) 349
AciI CCGC 3 cut(s) 347, 1766, 1986
AclWI GGATC 6 cut(s) 232, 245, 930, 1637, 1650, 2183
AcoI YGGCCR 2 cut(s) 1767, 1848
AcsI RAATTY 1 cut(s) 2230
AcuI CTGAAG 2 cut(s) 404, 2193
AcvI CACGTG 1 cut(s) 777
AfaI GTAC 7 cut(s) 100, 583, 1338, 1386, 1591, 2067, 2169
AfiI CCNNNNNNNGG 3 cut(s) 218, 439, 1901
AflIII ACRYGT 2 cut(s) 891, 1581
AhdI GACNNNNNGTC 1 cut(s) 1279
AjnI CCWGG 5 cut(s) 270, 340, 438, 1374, 2191
AjuI GAANNNNNNNTTGG 4 cut(s) 1844, 1876, 2166, 2198
AleI CACNNNNGTG 1 cut(s) 849
Alw26I GTCTC 6 cut(s) 1048, 1258, 1270, 1964, 2275, 2369
AlwI GGATC 6 cut(s) 232, 245, 930, 1637, 1650, 2183
AlwNI CAGNNNCTG 1 cut(s) 1838
AoxI GGCC 5 cut(s) 1197, 1609, 1767, 1848, 2298
ApaI GGGCCC 1 cut(s) 2302
ApeKI GCWGC 9 cut(s) 244, 247, 703, 724, 816, 1706, 1838, 1918, 2147
ApoI RAATTY 1 cut(s) 2230
Asp718I GGTACC 1 cut(s) 2167
AspLEI GCGC 2 cut(s) 1207, 1228
AspS9I GGNCC 7 cut(s) 344, 430, 442, 1159, 1946, 2298, 2299
AsuC2I CCSGG 1 cut(s) 910
AsuHPI GGTGA 5 cut(s) 974, 1037, 1364, 2198, 2236
AsuNHI GCTAGC 1 cut(s) 1982
AvaII GGWCC 5 cut(s) 344, 430, 442, 1159, 1946
AxyI CCTNAGG 1 cut(s) 762
BaeGI GKGCMC 1 cut(s) 2302
BaeI ACNNNNGTAYC 2 cut(s) 1581, 1614
BalI TGGCCA 1 cut(s) 1850
BamHI GGATCC 2 cut(s) 237, 1642
BanI GGYRCC 1 cut(s) 2167
BanII GRGCYC 2 cut(s) 910, 2302
BbrPI CACGTG 1 cut(s) 777
BbsI GAAGAC 1 cut(s) 1299
BbvI GCAGC 9 cut(s) 256, 259, 715, 736, 803, 1718, 1825, 1905, 2134
BccI CCATC 7 cut(s) 102, 176, 591, 1125, 1495, 1807, 1924
BceAI ACGGC 1 cut(s) 1017
BciT130I CCWGG 5 cut(s) 272, 342, 440, 1376, 2193
BciVI GTATCC 2 cut(s) 982, 1927
BclI TGATCA 3 cut(s) 621, 1009, 2023
BcnI CCSGG 1 cut(s) 910
BcoDI GTCTC 6 cut(s) 1048, 1258, 1270, 1964, 2275, 2369
BfaI CTAG 8 cut(s) 93, 102, 1169, 1466, 1730, 1745, 1943, 1983
BfmI CTRYAG 3 cut(s) 242, 519, 1669
BfuAI ACCTGC 1 cut(s) 1662
BfuI GTATCC 2 cut(s) 982, 1927
BglII AGATCT 1 cut(s) 2315
BmcAI AGTACT 1 cut(s) 1338
Bme1390I CCNGG 6 cut(s) 272, 342, 440, 910, 1376, 2193
Bme18I GGWCC 5 cut(s) 344, 430, 442, 1159, 1946
BmeRI GACNNNNNGTC 1 cut(s) 1279
BmgT120I GGNCC 7 cut(s) 344, 430, 442, 1159, 1946, 2298, 2299
BmiI GGNNCC 5 cut(s) 239, 1644, 2169, 2300, 2301
BmrFI CCNGG 6 cut(s) 272, 342, 440, 910, 1376, 2193
BmsI GCATC 7 cut(s) 326, 516, 1414, 1481, 1574, 1724, 2287
BmtI GCTAGC 1 cut(s) 1986
BoxI GACNNNNGTC 1 cut(s) 1169
BpiI GAAGAC 1 cut(s) 1299
BplI GAGNNNNNCTC 2 cut(s) 1371, 1403
BpmI CTGGAG 2 cut(s) 195, 1404
BpuEI CTTGAG 3 cut(s) 506, 1227, 2366
BpuMI CCSGG 1 cut(s) 910
BsaAI YACGTR 2 cut(s) 777, 851
BsaBI GATNNNNATC 1 cut(s) 1938
BsaI GGTCTC 1 cut(s) 1048
BsaJI CCNNGG 5 cut(s) 270, 426, 713, 985, 1375
BsaXI ACNNNNNCTCC 4 cut(s) 68, 98, 1716, 1746
Bsc4I CCNNNNNNNGG 3 cut(s) 218, 439, 1901
Bse1I ACTGG 2 cut(s) 1330, 1612
Bse21I CCTNAGG 1 cut(s) 762
Bse3DI GCAATG 3 cut(s) 78, 479, 707
Bse8I GATNNNNATC 1 cut(s) 1938
BseBI CCWGG 5 cut(s) 272, 342, 440, 1376, 2193
BseDI CCNNGG 5 cut(s) 270, 426, 713, 985, 1375
BseGI GGATG 6 cut(s) 201, 317, 918, 1136, 1343, 2293
BseJI GATNNNNATC 1 cut(s) 1938
BseLI CCNNNNNNNGG 3 cut(s) 218, 439, 1901
BseMI GCAATG 3 cut(s) 78, 479, 707
BseMII CTCAG 6 cut(s) 1092, 1146, 1871, 1959, 2127, 2286
BseNI ACTGG 2 cut(s) 1330, 1612
BseRI GAGGAG 2 cut(s) 419, 679
BseSI GKGCMC 1 cut(s) 2302
BseXI GCAGC 9 cut(s) 256, 259, 715, 736, 803, 1718, 1825, 1905, 2134
BsgI GTGCAG 1 cut(s) 1851
Bsh1236I CGCG 1 cut(s) 349
BshFI GGCC 5 cut(s) 1199, 1611, 1769, 1850, 2300
BshNI GGYRCC 1 cut(s) 2167
BsiSI CCGG 1 cut(s) 910
BslFI GGGAC 2 cut(s) 103, 253
BslI CCNNNNNNNGG 3 cut(s) 218, 439, 1901
BsmAI GTCTC 6 cut(s) 1048, 1258, 1270, 1964, 2275, 2369
BsmFI GGGAC 2 cut(s) 103, 253
BsnI GGCC 5 cut(s) 1199, 1611, 1769, 1850, 2300
Bso31I GGTCTC 1 cut(s) 1048
Bsp120I GGGCCC 1 cut(s) 2298
Bsp1286I GDGCHC 2 cut(s) 910, 2302
Bsp1407I TGTACA 1 cut(s) 581
Bsp19I CCATGG 1 cut(s) 985
BspACI CCGC 3 cut(s) 347, 1766, 1986
BspANI GGCC 5 cut(s) 1199, 1611, 1769, 1850, 2300
BspCNI CTCAG 6 cut(s) 1093, 1147, 1870, 1960, 2126, 2285
BspFNI CGCG 1 cut(s) 349
BspHI TCATGA 1 cut(s) 168
BspLI GGNNCC 5 cut(s) 239, 1644, 2169, 2300, 2301
BspMAI CTGCAG 2 cut(s) 246, 1673
BspMI ACCTGC 1 cut(s) 1662
BspOI GCTAGC 1 cut(s) 1986
BspPI GGATC 6 cut(s) 232, 245, 930, 1637, 1650, 2183
BspT107I GGYRCC 1 cut(s) 2167
BspTNI GGTCTC 1 cut(s) 1048
BsrDI GCAATG 3 cut(s) 78, 479, 707
BsrGI TGTACA 1 cut(s) 581
BsrI ACTGG 2 cut(s) 1330, 1612
BssECI CCNNGG 5 cut(s) 270, 426, 713, 985, 1375
BssT1I CCWWGG 2 cut(s) 426, 985
Bst2UI CCWGG 5 cut(s) 272, 342, 440, 1376, 2193
Bst4CI ACNGT 8 cut(s) 523, 639, 840, 1252, 1298, 1456, 1873, 2065
BstAPI GCANNNNNTGC 3 cut(s) 709, 1712, 1838
BstAUI TGTACA 1 cut(s) 581
BstBAI YACGTR 2 cut(s) 777, 851
BstC8I GCNNGC 8 cut(s) 747, 772, 1314, 1522, 1526, 1916, 1984, 2048
BstDEI CTNAG 9 cut(s) 762, 1101, 1155, 1857, 1968, 2113, 2272, 2334, 2351
BstDSI CCRYGG 1 cut(s) 985
BstF5I GGATG 6 cut(s) 201, 317, 918, 1136, 1343, 2293
BstFNI CGCG 1 cut(s) 349
BstHHI GCGC 2 cut(s) 1207, 1228
BstMAI GTCTC 6 cut(s) 1048, 1258, 1270, 1964, 2275, 2369
BstMWI GCNNNNNNNGC 9 cut(s) 77, 466, 709, 1712, 1838, 1847, 1924, 1973, 2086
BstNI CCWGG 5 cut(s) 272, 342, 440, 1376, 2193
BstNSI RCATGY 4 cut(s) 460, 895, 1585, 1918
BstPAI GACNNNNGTC 1 cut(s) 1169
BstSCI CCNGG 6 cut(s) 270, 340, 438, 908, 1374, 2191
BstSFI CTRYAG 3 cut(s) 242, 519, 1669
BstSLI GKGCMC 1 cut(s) 2302
BstUI CGCG 1 cut(s) 349
BstV1I GCAGC 9 cut(s) 256, 259, 715, 736, 803, 1718, 1825, 1905, 2134
BstV2I GAAGAC 1 cut(s) 1299
BstX2I RGATCY 3 cut(s) 237, 1642, 2315
BstXI CCANNNNNNTGG 1 cut(s) 1375
BstYI RGATCY 3 cut(s) 237, 1642, 2315
Bsu36I CCTNAGG 1 cut(s) 762
BsuI GTATCC 2 cut(s) 982, 1927
BsuRI GGCC 5 cut(s) 1199, 1611, 1769, 1850, 2300
BtgI CCRYGG 1 cut(s) 985
BtgZI GCGATG 1 cut(s) 1473
BtsCI GGATG 6 cut(s) 201, 317, 918, 1136, 1343, 2293
BtsI GCAGTG 1 cut(s) 1666
BtsIMutI CAGTG 3 cut(s) 1666, 2091, 2280
BveI ACCTGC 1 cut(s) 1662
Cac8I GCNNGC 8 cut(s) 747, 772, 1314, 1522, 1526, 1916, 1984, 2048
CaiI CAGNNNCTG 1 cut(s) 1838
CciI TCATGA 1 cut(s) 168
CfoI GCGC 2 cut(s) 1207, 1228
Cfr13I GGNCC 7 cut(s) 344, 430, 442, 1159, 1946, 2298, 2299
CseI GACGC 1 cut(s) 2205
Csp6I GTAC 7 cut(s) 99, 582, 1337, 1385, 1590, 2066, 2168
CviQI GTAC 7 cut(s) 99, 582, 1337, 1385, 1590, 2066, 2168
DdeI CTNAG 9 cut(s) 762, 1101, 1155, 1857, 1968, 2113, 2272, 2334, 2351
DriI GACNNNNNGTC 1 cut(s) 1279
EaeI YGGCCR 2 cut(s) 1767, 1848
Eam1105I GACNNNNNGTC 1 cut(s) 1279
Eco130I CCWWGG 2 cut(s) 426, 985
Eco24I GRGCYC 2 cut(s) 910, 2302
Eco31I GGTCTC 1 cut(s) 1048
Eco32I GATATC 1 cut(s) 2001
Eco47I GGWCC 5 cut(s) 344, 430, 442, 1159, 1946
Eco57I CTGAAG 2 cut(s) 404, 2193
Eco72I CACGTG 1 cut(s) 777
Eco81I CCTNAGG 1 cut(s) 762
EcoO109I RGGNCCY 2 cut(s) 2298, 2299
EcoRII CCWGG 5 cut(s) 270, 340, 438, 1374, 2191
EcoRV GATATC 1 cut(s) 2001
EcoT14I CCWWGG 2 cut(s) 426, 985
EcoT22I ATGCAT 2 cut(s) 509, 1567
EcoT38I GRGCYC 2 cut(s) 910, 2302
ErhI CCWWGG 2 cut(s) 426, 985
FalI AAGNNNNNCTT 2 cut(s) 867, 899
FaqI GGGAC 2 cut(s) 103, 253
FauI CCCGC 1 cut(s) 1979
FbaI TGATCA 3 cut(s) 621, 1009, 2023
FokI GGATG 6 cut(s) 208, 304, 925, 1143, 1330, 2300
FriOI GRGCYC 2 cut(s) 910, 2302
FspBI CTAG 8 cut(s) 93, 102, 1169, 1466, 1730, 1745, 1943, 1983
GlaI GCGC 2 cut(s) 1206, 1227
GsuI CTGGAG 2 cut(s) 195, 1404
HaeIII GGCC 5 cut(s) 1199, 1611, 1769, 1850, 2300
HapII CCGG 1 cut(s) 910
HgaI GACGC 1 cut(s) 2205
HhaI GCGC 2 cut(s) 1207, 1228
Hin6I GCGC 2 cut(s) 1205, 1226
HinP1I GCGC 2 cut(s) 1205, 1226
HindIII AAGCTT 2 cut(s) 231, 1192
HinfI GANTC 7 cut(s) 302, 566, 787, 943, 1165, 1725, 2039
HpaII CCGG 1 cut(s) 910
HphI GGTGA 5 cut(s) 974, 1037, 1364, 2198, 2236
Hpy166II GTNNAC 2 cut(s) 224, 2244
Hpy188I TCNGA 9 cut(s) 9, 301, 307, 312, 384, 400, 717, 1014, 1575
Hpy8I GTNNAC 2 cut(s) 224, 2244
Hpy99I CGWCG 1 cut(s) 902
HpyAV CCTTC 5 cut(s) 58, 379, 673, 1114, 2316
HpyCH4III ACNGT 8 cut(s) 523, 639, 840, 1252, 1298, 1456, 1873, 2065
HpyCH4IV ACGT 3 cut(s) 776, 850, 1441
HpyF10VI GCNNNNNNNGC 9 cut(s) 77, 466, 709, 1712, 1838, 1847, 1924, 1973, 2086
HpyF3I CTNAG 9 cut(s) 762, 1101, 1155, 1857, 1968, 2113, 2272, 2334, 2351
HpySE526I ACGT 3 cut(s) 776, 850, 1441
HspAI GCGC 2 cut(s) 1205, 1226
KpnI GGTACC 1 cut(s) 2171
Ksp22I TGATCA 3 cut(s) 621, 1009, 2023
LmnI GCTCC 2 cut(s) 214, 1576
Lsp1109I GCAGC 9 cut(s) 256, 259, 715, 736, 803, 1718, 1825, 1905, 2134
LweI GCATC 7 cut(s) 326, 516, 1414, 1481, 1574, 1724, 2287
MaeI CTAG 8 cut(s) 93, 102, 1169, 1466, 1730, 1745, 1943, 1983
MaeII ACGT 3 cut(s) 776, 850, 1441
MaeIII GTNAC 6 cut(s) 148, 329, 834, 842, 1090, 2103
MboII GAAGA 4 cut(s) 117, 431, 887, 1304
MfeI CAATTG 2 cut(s) 1005, 1533
MflI RGATCY 3 cut(s) 237, 1642, 2315
MhlI GDGCHC 2 cut(s) 910, 2302
MlsI TGGCCA 1 cut(s) 1850
MluNI TGGCCA 1 cut(s) 1850
MlyI GAGTC 3 cut(s) 560, 1159, 1734
MmeI TCCRAC 5 cut(s) 595, 912, 1596, 2206, 2336
Mox20I TGGCCA 1 cut(s) 1850
Mph1103I ATGCAT 2 cut(s) 509, 1567
MscI TGGCCA 1 cut(s) 1850
MseI TTAA 8 cut(s) 33, 359, 696, 930, 1353, 1677, 2109, 2238
MslI CAYNNNNRTG 3 cut(s) 829, 849, 951
Msp20I TGGCCA 1 cut(s) 1850
MspI CCGG 1 cut(s) 910
MspR9I CCNGG 6 cut(s) 272, 342, 440, 910, 1376, 2193
MunI CAATTG 2 cut(s) 1005, 1533
MvaI CCWGG 5 cut(s) 272, 342, 440, 1376, 2193
MvnI CGCG 1 cut(s) 349
MwoI GCNNNNNNNGC 9 cut(s) 77, 466, 709, 1712, 1838, 1847, 1924, 1973, 2086
NciI CCSGG 1 cut(s) 910
NcoI CCATGG 1 cut(s) 985
NheI GCTAGC 1 cut(s) 1982
NlaIV GGNNCC 5 cut(s) 239, 1644, 2169, 2300, 2301
NmuCI GTSAC 3 cut(s) 329, 842, 2103
NsiI ATGCAT 2 cut(s) 509, 1567
NspI RCATGY 4 cut(s) 460, 895, 1585, 1918
OliI CACNNNNGTG 1 cut(s) 849
PaeI GCATGC 1 cut(s) 1918
PagI TCATGA 1 cut(s) 168
PciI ACATGT 2 cut(s) 891, 1581
PcsI WCGNNNNNNNCGW 1 cut(s) 782
PfeI GAWTC 4 cut(s) 302, 787, 943, 2039
PflMI CCANNNNNTGG 2 cut(s) 439, 1901
PfoI TCCNGGA 1 cut(s) 2191
PleI GAGTC 3 cut(s) 560, 1159, 1733
PmaCI CACGTG 1 cut(s) 777
PmlI CACGTG 1 cut(s) 777
PpsI GAGTC 3 cut(s) 560, 1159, 1733
Ppu21I YACGTR 2 cut(s) 777, 851
PscI ACATGT 2 cut(s) 891, 1581
PshAI GACNNNNGTC 1 cut(s) 1169
Psp6I CCWGG 5 cut(s) 270, 340, 438, 1374, 2191
PspCI CACGTG 1 cut(s) 777
PspGI CCWGG 5 cut(s) 270, 340, 438, 1374, 2191
PspN4I GGNNCC 5 cut(s) 239, 1644, 2169, 2300, 2301
PspOMI GGGCCC 1 cut(s) 2298
PspPI GGNCC 7 cut(s) 344, 430, 442, 1159, 1946, 2298, 2299
PstI CTGCAG 2 cut(s) 246, 1673
PstNI CAGNNNCTG 1 cut(s) 1838
PsuI RGATCY 3 cut(s) 237, 1642, 2315
RsaI GTAC 7 cut(s) 100, 583, 1338, 1386, 1591, 2067, 2169
RsaNI GTAC 7 cut(s) 99, 582, 1337, 1385, 1590, 2066, 2168
RseI CAYNNNNRTG 3 cut(s) 829, 849, 951
SaqAI TTAA 8 cut(s) 33, 359, 696, 930, 1353, 1677, 2109, 2238
Sau96I GGNCC 7 cut(s) 344, 430, 442, 1159, 1946, 2298, 2299
ScaI AGTACT 1 cut(s) 1338
SchI GAGTC 3 cut(s) 560, 1159, 1734
ScrFI CCNGG 6 cut(s) 272, 342, 440, 910, 1376, 2193
SduI GDGCHC 2 cut(s) 910, 2302
SfaNI GCATC 7 cut(s) 326, 516, 1414, 1481, 1574, 1724, 2287
SfcI CTRYAG 3 cut(s) 242, 519, 1669
SinI GGWCC 5 cut(s) 344, 430, 442, 1159, 1946
SmiMI CAYNNNNRTG 3 cut(s) 829, 849, 951
SmlI CTYRAG 3 cut(s) 485, 1242, 2345
SmoI CTYRAG 3 cut(s) 485, 1242, 2345
SphI GCATGC 1 cut(s) 1918
SsiI CCGC 3 cut(s) 347, 1766, 1986
SspMI CTAG 8 cut(s) 93, 102, 1169, 1466, 1730, 1745, 1943, 1983
StyD4I CCNGG 6 cut(s) 270, 340, 438, 908, 1374, 2191
StyI CCWWGG 2 cut(s) 426, 985
TaaI ACNGT 8 cut(s) 523, 639, 840, 1252, 1298, 1456, 1873, 2065
TaiI ACGT 3 cut(s) 779, 853, 1444
TatI WGTACW 4 cut(s) 98, 581, 1336, 1384
TauI GCSGC 1 cut(s) 1769
TfiI GAWTC 4 cut(s) 302, 787, 943, 2039
Tru1I TTAA 8 cut(s) 33, 359, 696, 930, 1353, 1677, 2109, 2238
Tru9I TTAA 8 cut(s) 33, 359, 696, 930, 1353, 1677, 2109, 2238
TscAI CASTG 3 cut(s) 1673, 2091, 2280
TseFI GTSAC 3 cut(s) 329, 842, 2103
TseI GCWGC 9 cut(s) 244, 247, 703, 724, 816, 1706, 1838, 1918, 2147
Tsp45I GTSAC 3 cut(s) 329, 842, 2103
TspDTI ATGAA 8 cut(s) 157, 432, 668, 941, 1233, 1674, 1913, 2304
TspGWI ACGGA 2 cut(s) 487, 1680
TspRI CASTG 3 cut(s) 1673, 2091, 2280
Van91I CCANNNNNTGG 2 cut(s) 439, 1901
VpaK11BI GGWCC 5 cut(s) 344, 430, 442, 1159, 1946
XapI RAATTY 1 cut(s) 2230
XceI RCATGY 4 cut(s) 460, 895, 1585, 1918
XspI CTAG 8 cut(s) 93, 102, 1169, 1466, 1730, 1745, 1943, 1983
ZrmI AGTACT 1 cut(s) 1338
Zsp2I ATGCAT 2 cut(s) 509, 1567
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.