pycom17g13740
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0011195
Physical Location & Seq
Forward (+)
11309338 .. 11312611
3274 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3015 bp
ATGAAGCTTTACCCTTTCAATTTCTTCTTCTTCTTCTTCTTGTTATTCTGCTTCTCTGTGCCCATACCTATTGTATCTAGTTTGAGCTCTGATGGGGTGGCGCTGTTGTCCCTCCTCAAGCACTGGACTGTAATCCCATCCCCCATATCCTCCACCTGGAATGCCTCTGATTCCAATCCATGCCAATGGGTTGGAATCCAATGTGATAAATCCCACAATGTGGTTGCCTTAAATCTCACTGGGTTTGGAATTTCTGGCCAATTGGGGCCTCAAGTTGGCAGCTTTAGGAATAGTTTGAATGGTTCCATCCCTGCAAATGTTGGGAATTTGAGTAAATTGGAGGAGTTGTATTTGGATGACAACCATTTGAGTGGAGCCCTGCCTAAGAGTCTGAACAATCTTGGGAACCTAGTGTATCTTCAGGTTAGTGGTAATCGTCTCGACGGTAGGATTGATTTGGGTTCTGGGGATTGCAAGAATTTGATTTTCTTGGATTTGTCACGCAATCAGTTTAGTGGAGGTCTTCCATCAAGTCTGGGAAATTGTAGTAATTTAACAGAGTTTGGTGCTGTGAATAGCAACTTAACGGGGACTATCCCGTCTTCCTTTGGCCAACTAGAGAAGCTTGAACTTCTGCGCCTTCCTGAGAACCGTTTGTCTGGGAAAATACCTCCCGAACTTGGTAAATGCAAGTCCTTAACAGGACTACAGTTGTATACAAACCAACTTGAGGGAGAAATTCCTAGTGAATTGGGGACGCTGAAATTGCAGGATCTTGACTTGTTTGAGAACAGGTTAACTGGTGAAATCCCAGTTAGCATTTGGAAGATTCAGAGTCTTCAGCAAATCCTTGTGTACAATAATAACCTCACCGGGGAGCTGCCTGTAGAGATGACTGAGCTGAAGCAACTGCAGAATATTTCGTTGTTTAACAACCTCTTTTCTGGAGTTATACCTCAAGGTCTGGGGATTAACAGCAGTTTAGTGCAGTTAGATTTTCTGAATAATAACTTCACTGGTACAATCCCTCCAAATCTTTGCCATGGAAAGAGGTTAAGGGTGTTGACTTTGGCTTCCAATCGACTTCAAGGTTCCATACCTTCTGATGTTGGAAACTGCTCTACACTTTGGAGGTTGAAGCTTGAACAGAATAACCTGACCGGAGCTCTCCCGGAATTTGCAAAAAATCCAAACCTCAATTATATGGACATCAGCAGCAATGAGATAAGTGGAGCAATTCCATCAAGCTTGCAAAACTGTGGCAACCTCACAACCATCAATTTGTCCACGAATAGGTTAACTGAACCTATACCGCAGGAGCTGGGGAAGCTTGCAGAGCTTCGTACTTTGGTTCTTTTCCAGAACAATTTGATTGGTTCTCTGCCTCCTCAACTATCGAATTGTACCAAAATGGATAAGTTCGATGTGCGGTCCAATTTGTTGAATGGCTCCATTCCGTCAAGTCTGAGAAGTTGGACAGTTTTATCAACACTGATTTTAAGCGACAACAGCTTTACTGGCGGCATCCCAACTTTCTTGTCAGAGTTCGAAAAGCTTTCAGAGCTACAACTTGGTGGAAATCTATTCGGAGGTGTGATTCCATCAGCAATTGGAGCATTGCAGAGTATGTTTTATGCATTAAATCTTAGCAACAATGCATTGACAGGTCTGATTCCTTCAGAGCTAGGGAAACTGATAAGGCTCCAACGACTAGATCTTTCTCGTAACAATTTGACAGGGACTTTAAAAGTTCTCGGCGATATGAGTTCACTAACTGAGGTTAACGTTTCAGACAACAACTTCACAGGTCCAGTACCAGAGACATTGATGAACCTGTTGAGCTCATCCCCAGTGTCATTTTTGGGCAATCCCTACATATGTGTCAATTACCTTCCATCATGTGGCTCAATGTGCACAGGAAAGAACAGTTTTAAATCTTGCAACAGTCCACCAGGCAACCAGAAAGGCCTTAGTAAAGTGCAAATTGCATTTATAGCTCTAGGATCTTCGATGTTTGTTGTTTTTGTGCTTTATGGGCTGATTTATCTGTTCCTCTTGCGCAAAAAGACCAAGCACGACTTTGAGATCTCTGCTCTAGGGGGGCCATCTGCCTTGCTCAACACGGTACTGGAGGCTACAGAAAACCTAAATGATCACTATATCATTGGGAGAGGAGCCCATGGAACAGTCTATAAGGCCTCTTTGGCTCCAGACAAAGATTATGCAGTCAAGAAGCTTCAATTTGCAGGGCACGAAGGAAGGCGTTTGAGCATGATTAGAGAAATTCAAACACTTGGGTTGATTAGGCACCGGAATCTGGTTAGATTGGAAGACTTCTGGTTAAGAAAAGACCACGGTTTGATATTGTATCGGTACATGCAAAATGGGAGCCTTTATGATGTTTTACATGAAATCAAACCCCCGCCAACTCTCAAGTGGAGTGTCCGCTATAGGATAGCGCTTGGAACTGCATATGGGTTGGAATATCTCCATTATGATTGTGATCCCCCTATAGTGCATCGAGACATCAAACCAATGAACATCCTTTTGGACTCTGACATGGAGCCCCATATCGGTGATTTTGGTATTGCGAAACTTTTGGATCATCAGTCTTCTGCAGCAACATCCATCGCAGTTGTGGGTACAACCGGATATATTGCACCAGAAAATGCATTTCGAACAGCAAAGAGCGTGGAATCTGATGTGTACAGTTATGGGGTTGTTTTACTTGAGCTGATAACAAGAAAGAAGGCATTGGATCGATCATTTATGGAGCAAACTGACATTGTAGAATGGGCTAGGTCAGTGTGGAGCAGCACGGAACAAATTGATCAGATCGTCGATTCAAGCCTTAAGGAGGAACTTCTGGATTCAAACATCATGGATCAAGTTATTGACGCTCTTATGGTGGCTTTCAGATGTACTGAGAAAGATCCGAAAAGTAGACCCACAATGAGAGATGTGATCAAGCAATTGTTAGATGCAGATTCCAAAGTGAGAAGCATAAAGGGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0001653 GO:0002376 GO:0003674 GO:0003824 GO:0004383 GO:0004672 GO:0004888 GO:0005488 GO:0005575 GO:0005576 GO:0005623 GO:0005886 GO:0005911 GO:0006139 GO:0006163 GO:0006164 GO:0006182 GO:0006464 GO:0006468 GO:0006725 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009058 GO:0009117 GO:0009150 GO:0009152 GO:0009165 GO:0009187 GO:0009190 GO:0009259 GO:0009260 GO:0009506 GO:0009605 GO:0009607 GO:0009611 GO:0009617 GO:0009719 GO:0009725 GO:0009753 GO:0009975 GO:0009987 GO:0010033 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0016829 GO:0016849 GO:0018130 GO:0019199 GO:0019438 GO:0019538 GO:0019637 GO:0019693 GO:0023052 GO:0030054 GO:0033218 GO:0034641 GO:0034654 GO:0036211 GO:0038023 GO:0042221 GO:0042277 GO:0042742 GO:0043170 GO:0043207 GO:0043412 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044464 GO:0045087 GO:0046068 GO:0046390 GO:0046483 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0052652 GO:0055044 GO:0055086 GO:0060089 GO:0065007 GO:0071704 GO:0071944 GO:0072521 GO:0072522 GO:0090407 GO:0098542 GO:0140096 GO:1901135 GO:1901137 GO:1901293 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_6 LRR_8 Pkinase

Protein Analysis

1005

Amino Acids

110.44

Weight (kDa)

6.35

Isoelectric Point (pI)

36.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 29 - 69 4.5e-09 Leucine rich repeat N-terminal domain
LRR_14 PF23598 258 - 454 2.8e-08 Leucine-rich repeat region
LRR_8 PF13855 397 - 457 3e-08 Leucine rich repeat
Pkinase PF00069 719 - 991 3.8e-43 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 721 - 992 7.8e-37 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011195)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G73080
fragaria_vesca FvH4_6g37420 FvH4_6g37430
malus_domestica MD09G1158300.v1.1 MD17G1144300.v1.1
prunus_persica Prupe.3G167800_v2.0.a1 Prupe.3G167800_v2.0.a1 Prupe.3G167900_v2.0.a1
pyrus_communis pycom09g07690 pycom17g13740
rosa_chinensis RchiOBHm_Chr2g0150241
rosa_laevigata RLG00000020481
rosa_multiflora Rmu_ssc0000020.1_g000002
rosa_roxburghii Rroxscaffold_2G00097340
rosa_rugosa Rorug02G0420100
rosa_samantha Rh2AG479100 Rh2BG491400 Rh2CG465300 Rh2DG500200
rosa_wichuraiana Rw2G039090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 598
Acc16I TGCGCA 1 cut(s) 2060
AccB1I GGYRCC 1 cut(s) 2307
AccB7I CCANNNNNTGG 2 cut(s) 220, 1901
AccI GTMKAC 2 cut(s) 716, 2944
AciI CCGC 5 cut(s) 1313, 1429, 1521, 2423, 2446
AclI AACGTT 1 cut(s) 1785
AclWI GGATC 7 cut(s) 780, 2011, 2498, 2610, 2766, 2892, 2927
AcoI YGGCCR 2 cut(s) 256, 610
AcsI RAATTY 6 cut(s) 249, 325, 478, 738, 1175, 2283
AcuI CTGAAG 4 cut(s) 404, 824, 923, 1662
AdeI CACNNNGTG 1 cut(s) 220
AfeI AGCGCT 1 cut(s) 2460
AflII CTTAAG 1 cut(s) 2852
AjnI CCWGG 2 cut(s) 155, 1951
AjuI GAANNNNNNNTTGG 2 cut(s) 2531, 2563
Alw21I GWGCWC 4 cut(s) 89, 1168, 1844, 1916
Alw26I GTCTC 3 cut(s) 443, 1814, 2517
Alw44I GTGCAC 1 cut(s) 1912
AlwI GGATC 7 cut(s) 780, 2011, 2498, 2610, 2766, 2892, 2927
AlwNI CAGNNNCTG 1 cut(s) 1321
Aor51HI AGCGCT 1 cut(s) 2460
AoxI GGCC 6 cut(s) 256, 266, 610, 1966, 2102, 2196
ApaLI GTGCAC 1 cut(s) 1912
ApeKI GCWGC 5 cut(s) 279, 880, 1215, 2618, 2814
ApoI RAATTY 6 cut(s) 249, 325, 478, 738, 1175, 2283
AspLEI GCGC 4 cut(s) 103, 639, 2061, 2461
AspS9I GGNCC 4 cut(s) 266, 1431, 1808, 2102
AsuC2I CCSGG 2 cut(s) 874, 1172
AsuHPI GGTGA 3 cut(s) 815, 862, 2588
AsuII TTCGAA 2 cut(s) 1548, 2677
AvaII GGWCC 2 cut(s) 1431, 1808
BaeGI GKGCMC 3 cut(s) 63, 1916, 2253
BalI TGGCCA 2 cut(s) 258, 612
BanI GGYRCC 1 cut(s) 2307
BanII GRGCYC 6 cut(s) 89, 379, 1168, 1844, 2179, 2568
BbsI GAAGAC 5 cut(s) 515, 594, 830, 2337, 2604
Bbv12I GWGCWC 4 cut(s) 89, 1168, 1844, 1916
BbvI GCAGC 5 cut(s) 291, 867, 1227, 2630, 2826
BciT130I CCWGG 2 cut(s) 157, 1953
BclI TGATCA 3 cut(s) 2152, 2830, 2964
BcnI CCSGG 2 cut(s) 874, 1172
BcoDI GTCTC 3 cut(s) 443, 1814, 2517
BfmI CTRYAG 7 cut(s) 707, 885, 911, 2136, 2449, 2511, 2616
BfoI RGCGCY 2 cut(s) 104, 2462
BfrI CTTAAG 1 cut(s) 2852
BglI GCCNNNNNGGC 1 cut(s) 2204
BglII AGATCT 2 cut(s) 1714, 2085
BisI GCNGC 6 cut(s) 280, 881, 1216, 1522, 2619, 2815
BlsI GCNGC 6 cut(s) 281, 882, 1217, 1523, 2620, 2816
Bme1390I CCNGG 4 cut(s) 157, 874, 1172, 1953
Bme18I GGWCC 2 cut(s) 1431, 1808
BmgT120I GGNCC 4 cut(s) 266, 1431, 1808, 2102
BmrFI CCNGG 4 cut(s) 157, 874, 1172, 1953
BmrI ACTGGG 3 cut(s) 249, 806, 1844
BmsI GCATC 3 cut(s) 1533, 2527, 2971
BmuI ACTGGG 3 cut(s) 249, 806, 1844
BpiI GAAGAC 5 cut(s) 515, 594, 830, 2337, 2604
BpmI CTGGAG 3 cut(s) 966, 2150, 2193
Bpu14I TTCGAA 2 cut(s) 1548, 2677
BpuEI CTTGAG 6 cut(s) 101, 255, 749, 942, 2417, 2750
BpuMI CCSGG 2 cut(s) 874, 1172
Bsa29I ATCGAT 1 cut(s) 2761
BsaBI GATNNNNATC 2 cut(s) 174, 2502
BsaJI CCNNGG 4 cut(s) 873, 1042, 2179, 2353
BsaWI WCCGGW 3 cut(s) 1160, 2310, 2648
BsaXI ACNNNNNCTCC 4 cut(s) 332, 362, 1012, 1042
Bse1I ACTGG 9 cut(s) 128, 244, 805, 812, 1021, 1522, 1811, 1850, 2133
Bse3DI GCAATG 2 cut(s) 1225, 1616
Bse8I GATNNNNATC 2 cut(s) 174, 2502
BseBI CCWGG 2 cut(s) 157, 1953
BseCI ATCGAT 1 cut(s) 2761
BseDI CCNNGG 4 cut(s) 873, 1042, 2179, 2353
BseGI GGATG 7 cut(s) 137, 306, 361, 1524, 1844, 2541, 2624
BseJI GATNNNNATC 2 cut(s) 174, 2502
BseMI GCAATG 2 cut(s) 1225, 1616
BseMII CTCAG 5 cut(s) 636, 888, 1457, 1767, 2916
BseNI ACTGG 9 cut(s) 128, 244, 805, 812, 1021, 1522, 1811, 1850, 2133
BseRI GAGGAG 4 cut(s) 104, 356, 1377, 2187
BseSI GKGCMC 3 cut(s) 63, 1916, 2253
BseXI GCAGC 5 cut(s) 291, 867, 1227, 2630, 2826
BseYI CCCAGC 1 cut(s) 1321
BsgI GTGCAG 1 cut(s) 1007
BshFI GGCC 6 cut(s) 258, 268, 612, 1968, 2104, 2198
BshNI GGYRCC 1 cut(s) 2307
BshVI ATCGAT 1 cut(s) 2761
BsiHKAI GWGCWC 4 cut(s) 89, 1168, 1844, 1916
BsiSI CCGG 5 cut(s) 873, 1161, 1172, 2311, 2649
BslFI GGGAC 4 cut(s) 94, 604, 769, 1753
BsmAI GTCTC 3 cut(s) 443, 1814, 2517
BsmBI CGTCTC 1 cut(s) 443
BsmFI GGGAC 4 cut(s) 94, 604, 769, 1753
BsmI GAATGC 1 cut(s) 166
BsnI GGCC 6 cut(s) 258, 268, 612, 1968, 2104, 2198
Bsp119I TTCGAA 2 cut(s) 1548, 2677
Bsp1286I GDGCHC 9 cut(s) 63, 89, 379, 1168, 1844, 1916, 2179, 2253, 2568
Bsp1407I TGTACA 2 cut(s) 855, 2706
Bsp19I CCATGG 2 cut(s) 1042, 2179
BspACI CCGC 5 cut(s) 1313, 1429, 1521, 2423, 2446
BspANI GGCC 6 cut(s) 258, 268, 612, 1968, 2104, 2198
BspCNI CTCAG 5 cut(s) 637, 889, 1458, 1768, 2917
BspDI ATCGAT 1 cut(s) 2761
BspMAI CTGCAG 2 cut(s) 915, 2620
BspPI GGATC 7 cut(s) 780, 2011, 2498, 2610, 2766, 2892, 2927
BspT104I TTCGAA 2 cut(s) 1548, 2677
BspT107I GGYRCC 1 cut(s) 2307
BspTI CTTAAG 1 cut(s) 2852
BsrDI GCAATG 2 cut(s) 1225, 1616
BsrGI TGTACA 2 cut(s) 855, 2706
BsrI ACTGG 9 cut(s) 128, 244, 805, 812, 1021, 1522, 1811, 1850, 2133
BssECI CCNNGG 4 cut(s) 873, 1042, 2179, 2353
BssNAI GTATAC 1 cut(s) 717
BssT1I CCWWGG 2 cut(s) 1042, 2179
Bst1107I GTATAC 1 cut(s) 717
Bst2UI CCWGG 2 cut(s) 157, 1953
BstAFI CTTAAG 1 cut(s) 2852
BstAUI TGTACA 2 cut(s) 855, 2706
BstBI TTCGAA 2 cut(s) 1548, 2677
BstC8I GCNNGC 2 cut(s) 1250, 1332
BstDEI CTNAG 8 cut(s) 384, 645, 897, 1466, 1646, 1776, 1970, 2925
BstDSI CCRYGG 3 cut(s) 1042, 2179, 2353
BstENI CCTNNNNNAGG 1 cut(s) 2855
BstF5I GGATG 7 cut(s) 137, 306, 361, 1524, 1844, 2541, 2624
BstH2I RGCGCY 2 cut(s) 104, 2462
BstHHI GCGC 4 cut(s) 103, 639, 2061, 2461
BstMAI GTCTC 3 cut(s) 443, 1814, 2517
BstNI CCWGG 2 cut(s) 157, 1953
BstNSI RCATGY 1 cut(s) 2380
BstSCI CCNGG 4 cut(s) 155, 872, 1170, 1951
BstSFI CTRYAG 7 cut(s) 707, 885, 911, 2136, 2449, 2511, 2616
BstSLI GKGCMC 3 cut(s) 63, 1916, 2253
BstV1I GCAGC 5 cut(s) 291, 867, 1227, 2630, 2826
BstV2I GAAGAC 5 cut(s) 515, 594, 830, 2337, 2604
BstX2I RGATCY 5 cut(s) 772, 1714, 2003, 2085, 2932
BstXI CCANNNNNNTGG 3 cut(s) 186, 191, 371
BstYI RGATCY 5 cut(s) 772, 1714, 2003, 2085, 2932
BstZ17I GTATAC 1 cut(s) 717
Bsu15I ATCGAT 1 cut(s) 2761
BsuRI GGCC 6 cut(s) 258, 268, 612, 1968, 2104, 2198
BsuTUI ATCGAT 1 cut(s) 2761
BtgI CCRYGG 3 cut(s) 1042, 2179, 2353
BtgZI GCGATG 1 cut(s) 2614
BtsCI GGATG 7 cut(s) 137, 306, 361, 1524, 1844, 2541, 2624
BtsIMutI CAGTG 6 cut(s) 121, 237, 1014, 1490, 1857, 2811
Cac8I GCNNGC 2 cut(s) 1250, 1332
CaiI CAGNNNCTG 1 cut(s) 1321
CfoI GCGC 4 cut(s) 103, 639, 2061, 2461
Cfr13I GGNCC 4 cut(s) 266, 1431, 1808, 2102
ClaI ATCGAT 1 cut(s) 2761
CseI GACGC 2 cut(s) 766, 2906
CspCI CAANNNNNGTGG 2 cut(s) 2673, 2708
CviAII CATG 9 cut(s) 180, 1043, 1899, 2180, 2272, 2377, 2408, 2560, 2881
DdeI CTNAG 8 cut(s) 384, 645, 897, 1466, 1646, 1776, 1970, 2925
DraI TTTAAA 2 cut(s) 1746, 1933
DraIII CACNNNGTG 1 cut(s) 220
DrdI GACNNNNNNGTC 1 cut(s) 598
DseDI GACNNNNNNGTC 1 cut(s) 598
EaeI YGGCCR 2 cut(s) 256, 610
Ecl136II GAGCTC 3 cut(s) 87, 1166, 1842
Eco130I CCWWGG 2 cut(s) 1042, 2179
Eco147I AGGCCT 2 cut(s) 1968, 2198
Eco24I GRGCYC 6 cut(s) 89, 379, 1168, 1844, 2179, 2568
Eco47I GGWCC 2 cut(s) 1431, 1808
Eco47III AGCGCT 1 cut(s) 2460
Eco53kI GAGCTC 3 cut(s) 87, 1166, 1842
Eco57I CTGAAG 4 cut(s) 404, 824, 923, 1662
EcoICRI GAGCTC 3 cut(s) 87, 1166, 1842
EcoNI CCTNNNNNAGG 1 cut(s) 2855
EcoO109I RGGNCCY 1 cut(s) 266
EcoRII CCWGG 2 cut(s) 155, 1951
EcoT14I CCWWGG 2 cut(s) 1042, 2179
EcoT22I ATGCAT 3 cut(s) 1639, 1660, 2674
EcoT38I GRGCYC 6 cut(s) 89, 379, 1168, 1844, 2179, 2568
ErhI CCWWGG 2 cut(s) 1042, 2179
Esp3I CGTCTC 1 cut(s) 443
FaeI CATG 9 cut(s) 183, 1046, 1902, 2183, 2275, 2380, 2411, 2563, 2884
FalI AAGNNNNNCTT 2 cut(s) 2063, 2095
FaqI GGGAC 4 cut(s) 94, 604, 769, 1753
FatI CATG 9 cut(s) 179, 1042, 1898, 2179, 2271, 2376, 2407, 2559, 2880
FauI CCCGC 1 cut(s) 2430
FauNDI CATATG 2 cut(s) 1877, 2473
FbaI TGATCA 3 cut(s) 2152, 2830, 2964
FblI GTMKAC 2 cut(s) 716, 2944
Fnu4HI GCNGC 6 cut(s) 280, 881, 1216, 1522, 2619, 2815
FokI GGATG 7 cut(s) 124, 293, 368, 1511, 1831, 2528, 2611
FriOI GRGCYC 6 cut(s) 89, 379, 1168, 1844, 2179, 2568
Fsp4HI GCNGC 6 cut(s) 280, 881, 1216, 1522, 2619, 2815
FspI TGCGCA 1 cut(s) 2060
GlaI GCGC 4 cut(s) 102, 638, 2060, 2460
GluI GCNGC 6 cut(s) 280, 881, 1216, 1522, 2619, 2815
GsaI CCCAGC 1 cut(s) 1325
GsuI CTGGAG 3 cut(s) 966, 2150, 2193
HaeII RGCGCY 2 cut(s) 104, 2462
HaeIII GGCC 6 cut(s) 258, 268, 612, 1968, 2104, 2198
HapII CCGG 5 cut(s) 873, 1161, 1172, 2311, 2649
HgaI GACGC 2 cut(s) 766, 2906
HhaI GCGC 4 cut(s) 103, 639, 2061, 2461
Hin1II CATG 9 cut(s) 183, 1046, 1902, 2183, 2275, 2380, 2411, 2563, 2884
Hin6I GCGC 4 cut(s) 101, 637, 2059, 2459
HinP1I GCGC 4 cut(s) 101, 637, 2059, 2459
HincII GTYRAC 4 cut(s) 798, 1065, 1299, 1783
HindII GTYRAC 4 cut(s) 798, 1065, 1299, 1783
HindIII AAGCTT 7 cut(s) 5, 623, 1139, 1246, 1328, 1553, 2234
HpaI GTTAAC 3 cut(s) 798, 1299, 1783
HpaII CCGG 5 cut(s) 873, 1161, 1172, 2311, 2649
HphI GGTGA 3 cut(s) 815, 862, 2588
Hpy99I CGWCG 2 cut(s) 446, 2843
HpyAV CCTTC 7 cut(s) 650, 1110, 1686, 1901, 2249, 2253, 2743
HpyCH4IV ACGT 1 cut(s) 1785
HpyF3I CTNAG 8 cut(s) 384, 645, 897, 1466, 1646, 1776, 1970, 2925
HpySE526I ACGT 1 cut(s) 1785
Hsp92II CATG 9 cut(s) 183, 1046, 1902, 2183, 2275, 2380, 2411, 2563, 2884
HspAI GCGC 4 cut(s) 101, 637, 2059, 2459
Ksp22I TGATCA 3 cut(s) 2152, 2830, 2964
KspAI GTTAAC 3 cut(s) 798, 1299, 1783
Lsp1109I GCAGC 5 cut(s) 291, 867, 1227, 2630, 2826
LweI GCATC 3 cut(s) 1533, 2527, 2971
MaeII ACGT 1 cut(s) 1785
MaeIII GTNAC 2 cut(s) 498, 1724
MfeI CAATTG 3 cut(s) 260, 1608, 2972
MflI RGATCY 5 cut(s) 772, 1714, 2003, 2085, 2932
MhlI GDGCHC 9 cut(s) 63, 89, 379, 1168, 1844, 1916, 2179, 2253, 2568
MlsI TGGCCA 2 cut(s) 258, 612
MluNI TGGCCA 2 cut(s) 258, 612
MlyI GAGTC 3 cut(s) 397, 844, 2546
MmeI TCCRAC 5 cut(s) 172, 1090, 1454, 1729, 2460
Mox20I TGGCCA 2 cut(s) 258, 612
Mph1103I ATGCAT 3 cut(s) 1639, 1660, 2674
MscI TGGCCA 2 cut(s) 258, 612
MslI CAYNNNNRTG 3 cut(s) 184, 369, 2574
Msp20I TGGCCA 2 cut(s) 258, 612
MspCI CTTAAG 1 cut(s) 2852
MspI CCGG 5 cut(s) 873, 1161, 1172, 2311, 2649
MspR9I CCNGG 4 cut(s) 157, 874, 1172, 1953
MunI CAATTG 3 cut(s) 260, 1608, 2972
Mva1269I GAATGC 1 cut(s) 166
MvaI CCWGG 2 cut(s) 157, 1953
NciI CCSGG 2 cut(s) 874, 1172
NcoI CCATGG 2 cut(s) 1042, 2179
NdeI CATATG 2 cut(s) 1877, 2473
NlaIII CATG 9 cut(s) 183, 1046, 1902, 2183, 2275, 2380, 2411, 2563, 2884
NmeAIII GCCGAG 1 cut(s) 1734
NmuCI GTSAC 1 cut(s) 498
NsbI TGCGCA 1 cut(s) 2060
NsiI ATGCAT 3 cut(s) 1639, 1660, 2674
NspI RCATGY 1 cut(s) 2380
NspV TTCGAA 2 cut(s) 1548, 2677
PceI AGGCCT 2 cut(s) 1968, 2198
PctI GAATGC 1 cut(s) 166
PflMI CCANNNNNTGG 2 cut(s) 220, 1901
PfoI TCCNGGA 1 cut(s) 1170
PkrI GCNGC 6 cut(s) 281, 882, 1217, 1523, 2620, 2816
PleI GAGTC 3 cut(s) 396, 843, 2546
PpsI GAGTC 3 cut(s) 396, 843, 2546
Psp124BI GAGCTC 3 cut(s) 89, 1168, 1844
Psp1406I AACGTT 1 cut(s) 1785
Psp6I CCWGG 2 cut(s) 155, 1951
PspFI CCCAGC 1 cut(s) 1321
PspGI CCWGG 2 cut(s) 155, 1951
PspPI GGNCC 4 cut(s) 266, 1431, 1808, 2102
PstI CTGCAG 2 cut(s) 915, 2620
PstNI CAGNNNCTG 1 cut(s) 1321
PsuI RGATCY 5 cut(s) 772, 1714, 2003, 2085, 2932
RseI CAYNNNNRTG 3 cut(s) 184, 369, 2574
SacI GAGCTC 3 cut(s) 89, 1168, 1844
SatI GCNGC 6 cut(s) 280, 881, 1216, 1522, 2619, 2815
Sau96I GGNCC 4 cut(s) 266, 1431, 1808, 2102
SchI GAGTC 3 cut(s) 397, 844, 2546
ScrFI CCNGG 4 cut(s) 157, 874, 1172, 1953
SduI GDGCHC 9 cut(s) 63, 89, 379, 1168, 1844, 1916, 2179, 2253, 2568
SfaNI GCATC 3 cut(s) 1533, 2527, 2971
SfcI CTRYAG 7 cut(s) 707, 885, 911, 2136, 2449, 2511, 2616
SfuI TTCGAA 2 cut(s) 1548, 2677
SinI GGWCC 2 cut(s) 1431, 1808
SmiMI CAYNNNNRTG 3 cut(s) 184, 369, 2574
SmlI CTYRAG 7 cut(s) 116, 270, 728, 957, 2432, 2729, 2852
SmoI CTYRAG 7 cut(s) 116, 270, 728, 957, 2432, 2729, 2852
SseBI AGGCCT 2 cut(s) 1968, 2198
SsiI CCGC 5 cut(s) 1313, 1429, 1521, 2423, 2446
SspI AATATT 1 cut(s) 919
SstI GAGCTC 3 cut(s) 89, 1168, 1844
StuI AGGCCT 2 cut(s) 1968, 2198
StyD4I CCNGG 4 cut(s) 155, 872, 1170, 1951
StyI CCWWGG 2 cut(s) 1042, 2179
TaiI ACGT 1 cut(s) 1788
TatI WGTACW 3 cut(s) 855, 2706, 2921
TauI GCSGC 1 cut(s) 1524
TscAI CASTG 6 cut(s) 128, 244, 1021, 1497, 1857, 2811
TseFI GTSAC 1 cut(s) 498
TseI GCWGC 5 cut(s) 279, 880, 1215, 2618, 2814
Tsp45I GTSAC 1 cut(s) 498
TspDTI ATGAA 4 cut(s) 17, 1844, 2424, 2552
TspGWI ACGGA 2 cut(s) 1446, 2834
TspRI CASTG 6 cut(s) 128, 244, 1021, 1497, 1857, 2811
Van91I CCANNNNNTGG 2 cut(s) 220, 1901
Vha464I CTTAAG 1 cut(s) 2852
VneI GTGCAC 1 cut(s) 1912
VpaK11BI GGWCC 2 cut(s) 1431, 1808
XagI CCTNNNNNAGG 1 cut(s) 2855
XapI RAATTY 6 cut(s) 249, 325, 478, 738, 1175, 2283
XceI RCATGY 1 cut(s) 2380
XcmI CCANNNNNNNNNTGG 3 cut(s) 819, 2433, 2635
XmiI GTMKAC 2 cut(s) 716, 2944
Zsp2I ATGCAT 3 cut(s) 1639, 1660, 2674
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.