Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr17

Orthogroup ID

OG0011230

Physical Location & Seq

Genomic Coordinates Reverse (-)

12497955 .. 12498896

Gene Structure 942 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom17g14760.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 942 bp

ATGGGAGACTCCGCTTTGCGTCGAACACTGGACGACTCGAACGCCGTAGAACTCACCGGAAAAATCATGATGATCGCCGTGTTGGTTCTCTTTCTGGTAGTCGTCTTCGTCCTCTGCCTCCACCTCTACGCCAAATGGTACTTGTGGCCGGCAGACGACGACCTCACCTCGTCAGTTCGCGGCCAGTCCCGACGCCGTCGCCGCCGCTTCGTTTTCGCTCCCGGGCAGGATCCAGTTGCCGCAGTGCGGAAAGGGCTCGACCCTTTAGTCCTCAGGTCCTTACCATTAGTAGTGTTTCCTTCCGATGACTTCAAAGACGGTCTCGAATGCGCGGTTTGTCTCTCCGAGCTCGTCCACGGCGAGAAAGCAAGGCTGCTCCCGAAATGCAGCCATGGATTCCATGTCGATTGTATCGACATGTGGTTTCAGTCTCACTCCACGTGTCCACTCTGTAGAAACCCTGTTGCTCCTCAGAGCTCTGTCGGTTCTAGTTCTTCTGCATTGGAAGAGAGTGCTGGTGGTGTTGAAATTCAATCACCAGCTGAGAATTTGGCATCTGGGTTTTCGATTGAGGCTCCGATTTTCCCAACAAATGTGTTGTTCTGGGGGAACCAGAGCCGGGTGAGCTCCGGACCGGGTTCGGGTTTGGAGGAAGAAGGCCCTTCTTCACAACCCAGCCCATCTCCATCATCTTGGTCATCTGCAAGTAATAGGCCGGATGGGATGTTGGTCATTGATATACCAAGCGAGACACCATCTGCTTCGCCTTCGCCTTCGCCTTCAAGTAACAGGTTTGCCGGAGAGGAGGACTTGAAGTCACCGGTGCTGACGCGGTTGAGATCACTGAAGAGGCTGTTGAGCAGGGATAGGAGGATTAGTCCCTGCAGTCCTTGTTCTGTGGACGTTGAACAAGGAGGAGGGAGAGGAGGAGGGCAGAGTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009987 GO:0010498 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019941 GO:0030163 GO:0032446 GO:0036211 GO:0043161 GO:0043170 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0051603 GO:0061630 GO:0061659 GO:0070647 GO:0071704 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

zf-RING_2

Protein Analysis

314

Amino Acids

33.63

Weight (kDa)

6.0

Isoelectric Point (pI)

83.66

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-RING_2	PF13639	109 - 152	2.8e-13	Ring finger domain
zf-RING_11	PF17123	109 - 138	1.9e-10	RING-like zinc finger
zf-C3HC4_2	PF13923	109 - 151	9.6e-07	Zinc finger, C3HC4 type (RING finger)
zf-rbx1	PF12678	125 - 152	3.8e-07	RING-H2 zinc finger domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011230)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G53820 AT1G72310
fragaria_vesca	FvH4_6g35980
malus_domestica	MD09G1172100.v1.1 MD17G1154800.v1.1
prunus_persica	Prupe.3G030500_v2.0.a1
pyrus_communis	pycom09g08970 pycom17g14760
rosa_chinensis	RchiOBHm_Chr2g0148101
rosa_laevigata	RLG00000020313
rosa_multiflora	Rmu_co8467879.1_g000001 Rmu_sc0001516.1_g000036 Rmu_sc0032339.1_g000001
rosa_roxburghii	Rroxscaffold_2G00099150
rosa_rugosa	Rorug02G0403500
rosa_samantha	Rh2AG461300 Rh2BG475100 Rh2CG448400 Rh2DG483700
rosa_wichuraiana	Rw2G037660

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	266
AccII	CGCG	3 cut(s)	180, 332, 832
AccIII	TCCGGA	1 cut(s)	629
AciI	CCGC	8 cut(s)	12, 180, 202, 205, 240, 247, 332, 832
AclWI	GGATC	2 cut(s)	224, 237
AcoI	YGGCCR	2 cut(s)	146, 181
AcsI	RAATTY	2 cut(s)	528, 547
AcuI	CTGAAG	1 cut(s)	866
AcvI	CACGTG	1 cut(s)	441
AcyI	GRCGYC	1 cut(s)	193
AfaI	GTAC	1 cut(s)	140
AfiI	CCNNNNNNNGG	3 cut(s)	246, 619, 641
AflIII	ACRYGT	2 cut(s)	417, 440
AgeI	ACCGGT	1 cut(s)	820
AgsI	TTSAA	6 cut(s)	313, 527, 533, 783, 814, 908
AhdI	GACNNNNNGTC	1 cut(s)	814
AluBI	AGCT	4 cut(s)	349, 477, 542, 627
AluI	AGCT	4 cut(s)	349, 477, 542, 627
Alw21I	GWGCWC	3 cut(s)	351, 479, 629
Alw26I	GTCTC	4 cut(s)	326, 344, 435, 743
AlwI	GGATC	2 cut(s)	224, 237
Ama87I	CYCGRG	1 cut(s)	221
Aor13HI	TCCGGA	1 cut(s)	629
AoxI	GGCC	4 cut(s)	146, 181, 658, 713
ApeKI	GCWGC	2 cut(s)	373, 387
ApoI	RAATTY	2 cut(s)	528, 547
AsiGI	ACCGGT	1 cut(s)	820
AspLEI	GCGC	1 cut(s)	332
AspS9I	GGNCC	3 cut(s)	276, 632, 659
AsuC2I	CCSGG	4 cut(s)	222, 223, 620, 636
AsuHPI	GGTGA	5 cut(s)	46, 157, 528, 634, 810
AvaI	CYCGRG	1 cut(s)	221
AvaII	GGWCC	2 cut(s)	276, 632
AxyI	CCTNAGG	1 cut(s)	272
BamHI	GGATCC	1 cut(s)	229
BanII	GRGCYC	4 cut(s)	258, 351, 479, 629
BbrPI	CACGTG	1 cut(s)	441
BbsI	GAAGAC	1 cut(s)	97
Bbv12I	GWGCWC	3 cut(s)	351, 479, 629
BbvI	GCAGC	2 cut(s)	360, 399
BccI	CCATC	4 cut(s)	688, 694, 713, 763
BceAI	ACGGC	4 cut(s)	29, 62, 180, 373
BcnI	CCSGG	4 cut(s)	222, 223, 620, 636
BcoDI	GTCTC	4 cut(s)	326, 344, 435, 743
BfaI	CTAG	1 cut(s)	489
BfmI	CTRYAG	2 cut(s)	451, 883
BisI	GCNGC	6 cut(s)	181, 202, 205, 240, 374, 388
BlsI	GCNGC	6 cut(s)	182, 203, 206, 241, 375, 389
Bme1390I	CCNGG	4 cut(s)	222, 223, 620, 636
Bme18I	GGWCC	2 cut(s)	276, 632
BmeRI	GACNNNNNGTC	1 cut(s)	814
BmeT110I	CYCGRG	1 cut(s)	221
BmgT120I	GGNCC	3 cut(s)	276, 632, 659
BmiI	GGNNCC	3 cut(s)	231, 576, 611
BmrFI	CCNGG	4 cut(s)	222, 223, 620, 636
BmsI	GCATC	1 cut(s)	563
BpiI	GAAGAC	1 cut(s)	97
BpuMI	CCSGG	4 cut(s)	222, 223, 620, 636
BsaAI	YACGTR	1 cut(s)	441
BsaHI	GRCGYC	1 cut(s)	193
BsaI	GGTCTC	1 cut(s)	326
BsaJI	CCNNGG	3 cut(s)	221, 355, 391
BsaWI	WCCGGW	3 cut(s)	56, 629, 820
BsaXI	ACNNNNNCTCC	2 cut(s)	862, 892
Bsc4I	CCNNNNNNNGG	3 cut(s)	246, 619, 641
Bse118I	RCCGGY	2 cut(s)	148, 820
Bse1I	ACTGG	3 cut(s)	33, 184, 233
Bse21I	CCTNAGG	1 cut(s)	272
BseAI	TCCGGA	1 cut(s)	629
BseDI	CCNNGG	3 cut(s)	221, 355, 391
BseGI	GGATG	2 cut(s)	724, 729
BseLI	CCNNNNNNNGG	3 cut(s)	246, 619, 641
BseMII	CTCAG	3 cut(s)	286, 485, 534
BseNI	ACTGG	3 cut(s)	33, 184, 233
BseRI	GAGGAG	5 cut(s)	459, 818, 930, 939, 942
BseXI	GCAGC	2 cut(s)	360, 399
BseYI	CCCAGC	1 cut(s)	674
Bsh1236I	CGCG	3 cut(s)	180, 332, 832
BshFI	GGCC	4 cut(s)	148, 183, 660, 715
BshTI	ACCGGT	1 cut(s)	820
BsiHKAI	GWGCWC	3 cut(s)	351, 479, 629
BsiHKCI	CYCGRG	1 cut(s)	221
BsiSI	CCGG	9 cut(s)	57, 149, 222, 619, 630, 635, 716, 798, 821
BslFI	GGGAC	2 cut(s)	172, 864
BslI	CCNNNNNNNGG	3 cut(s)	246, 619, 641
BsmAI	GTCTC	4 cut(s)	326, 344, 435, 743
BsmFI	GGGAC	2 cut(s)	172, 864
BsmI	GAATGC	1 cut(s)	332
BsnI	GGCC	4 cut(s)	148, 183, 660, 715
Bso31I	GGTCTC	1 cut(s)	326
BsoBI	CYCGRG	1 cut(s)	221
Bsp1286I	GDGCHC	4 cut(s)	258, 351, 479, 629
Bsp13I	TCCGGA	1 cut(s)	629
Bsp143I	GATC	3 cut(s)	72, 229, 839
Bsp19I	CCATGG	1 cut(s)	391
BspACI	CCGC	8 cut(s)	12, 180, 202, 205, 240, 247, 332, 832
BspANI	GGCC	4 cut(s)	148, 183, 660, 715
BspCNI	CTCAG	3 cut(s)	285, 484, 535
BspEI	TCCGGA	1 cut(s)	629
BspFNI	CGCG	3 cut(s)	180, 332, 832
BspHI	TCATGA	1 cut(s)	66
BspLI	GGNNCC	3 cut(s)	231, 576, 611
BspMAI	CTGCAG	1 cut(s)	887
BspPI	GGATC	2 cut(s)	224, 237
BspTNI	GGTCTC	1 cut(s)	326
BsrFI	RCCGGY	2 cut(s)	148, 820
BsrI	ACTGG	3 cut(s)	33, 184, 233
BssAI	RCCGGY	2 cut(s)	148, 820
BssECI	CCNNGG	3 cut(s)	221, 355, 391
BssMI	GATC	3 cut(s)	72, 229, 839
BssNI	GRCGYC	1 cut(s)	193
BssT1I	CCWWGG	1 cut(s)	391
Bst4CI	ACNGT	1 cut(s)	320
Bst6I	CTCTTC	2 cut(s)	501, 842
BstACI	GRCGYC	1 cut(s)	193
BstBAI	YACGTR	1 cut(s)	441
BstC8I	GCNNGC	1 cut(s)	150
BstDEI	CTNAG	3 cut(s)	272, 471, 543
BstDSI	CCRYGG	2 cut(s)	355, 391
BstF5I	GGATG	2 cut(s)	724, 729
BstFNI	CGCG	3 cut(s)	180, 332, 832
BstHHI	GCGC	1 cut(s)	332
BstKTI	GATC	3 cut(s)	75, 232, 842
BstMAI	GTCTC	4 cut(s)	326, 344, 435, 743
BstMBI	GATC	3 cut(s)	72, 229, 839
BstMWI	GCNNNNNNNGC	3 cut(s)	201, 253, 624
BstNSI	RCATGY	1 cut(s)	421
BstSCI	CCNGG	4 cut(s)	220, 221, 618, 634
BstSFI	CTRYAG	2 cut(s)	451, 883
BstUI	CGCG	3 cut(s)	180, 332, 832
BstV1I	GCAGC	2 cut(s)	360, 399
BstV2I	GAAGAC	1 cut(s)	97
BstX2I	RGATCY	1 cut(s)	229
BstXI	CCANNNNNNTGG	1 cut(s)	693
BstYI	RGATCY	1 cut(s)	229
Bsu36I	CCTNAGG	1 cut(s)	272
BsuRI	GGCC	4 cut(s)	148, 183, 660, 715
BtgI	CCRYGG	2 cut(s)	355, 391
BtsCI	GGATG	2 cut(s)	724, 729
BtsI	GCAGTG	1 cut(s)	249
BtsIMutI	CAGTG	3 cut(s)	26, 249, 842
Cac8I	GCNNGC	1 cut(s)	150
CciI	TCATGA	1 cut(s)	66
CfoI	GCGC	1 cut(s)	332
Cfr10I	RCCGGY	2 cut(s)	148, 820
Cfr13I	GGNCC	3 cut(s)	276, 632, 659
Cfr9I	CCCGGG	1 cut(s)	221
CpoI	CGGWCCG	1 cut(s)	632
CseI	GACGC	3 cut(s)	8, 201, 838
Csp6I	GTAC	1 cut(s)	139
CspAI	ACCGGT	1 cut(s)	820
CspI	CGGWCCG	1 cut(s)	632
CviAII	CATG	4 cut(s)	67, 392, 401, 418
CviQI	GTAC	1 cut(s)	139
DdeI	CTNAG	3 cut(s)	272, 471, 543
DpnI	GATC	3 cut(s)	74, 231, 841
DpnII	GATC	3 cut(s)	72, 229, 839
DrdI	GACNNNNNNGTC	1 cut(s)	266
DriI	GACNNNNNGTC	1 cut(s)	814
DseDI	GACNNNNNNGTC	1 cut(s)	266
EaeI	YGGCCR	2 cut(s)	146, 181
Eam1104I	CTCTTC	2 cut(s)	501, 842
Eam1105I	GACNNNNNGTC	1 cut(s)	814
EarI	CTCTTC	2 cut(s)	501, 842
Ecl136II	GAGCTC	3 cut(s)	349, 477, 627
Eco130I	CCWWGG	1 cut(s)	391
Eco24I	GRGCYC	4 cut(s)	258, 351, 479, 629
Eco31I	GGTCTC	1 cut(s)	326
Eco47I	GGWCC	2 cut(s)	276, 632
Eco53kI	GAGCTC	3 cut(s)	349, 477, 627
Eco57I	CTGAAG	1 cut(s)	866
Eco72I	CACGTG	1 cut(s)	441
Eco81I	CCTNAGG	1 cut(s)	272
Eco88I	CYCGRG	1 cut(s)	221
EcoICRI	GAGCTC	3 cut(s)	349, 477, 627
EcoO109I	RGGNCCY	2 cut(s)	276, 659
EcoT14I	CCWWGG	1 cut(s)	391
EcoT38I	GRGCYC	4 cut(s)	258, 351, 479, 629
ErhI	CCWWGG	1 cut(s)	391
FaeI	CATG	4 cut(s)	70, 395, 404, 421
FaiI	YATR	5 cut(s)	68, 393, 402, 419, 740
FaqI	GGGAC	2 cut(s)	172, 864
FatI	CATG	4 cut(s)	66, 391, 400, 417
Fnu4HI	GCNGC	6 cut(s)	181, 202, 205, 240, 374, 388
FokI	GGATG	2 cut(s)	731, 736
FriOI	GRGCYC	4 cut(s)	258, 351, 479, 629
Fsp4HI	GCNGC	6 cut(s)	181, 202, 205, 240, 374, 388
FspBI	CTAG	1 cut(s)	489
GlaI	GCGC	1 cut(s)	331
GluI	GCNGC	6 cut(s)	181, 202, 205, 240, 374, 388
GsaI	CCCAGC	1 cut(s)	678
HaeIII	GGCC	4 cut(s)	148, 183, 660, 715
HapII	CCGG	9 cut(s)	57, 149, 222, 619, 630, 635, 716, 798, 821
HgaI	GACGC	3 cut(s)	8, 201, 838
HhaI	GCGC	1 cut(s)	332
Hin1I	GRCGYC	1 cut(s)	193
Hin1II	CATG	4 cut(s)	70, 395, 404, 421
Hin6I	GCGC	1 cut(s)	330
HinP1I	GCGC	1 cut(s)	330
HinfI	GANTC	3 cut(s)	8, 35, 396
HpaII	CCGG	9 cut(s)	57, 149, 222, 619, 630, 635, 716, 798, 821
HphI	GGTGA	5 cut(s)	46, 157, 528, 634, 810
Hpy166II	GTNNAC	3 cut(s)	355, 446, 901
Hpy188I	TCNGA	4 cut(s)	304, 346, 474, 579
Hpy188III	TCNNGA	5 cut(s)	67, 189, 323, 379, 630
Hpy8I	GTNNAC	3 cut(s)	355, 446, 901
Hpy99I	CGWCG	4 cut(s)	24, 161, 195, 201
HpyAV	CCTTC	6 cut(s)	309, 650, 672, 777, 783, 789
HpyCH4III	ACNGT	1 cut(s)	320
HpyCH4IV	ACGT	2 cut(s)	440, 903
HpyCH4V	TGCA	4 cut(s)	387, 500, 704, 885
HpyF10VI	GCNNNNNNNGC	3 cut(s)	201, 253, 624
HpyF3I	CTNAG	3 cut(s)	272, 471, 543
HpySE526I	ACGT	2 cut(s)	440, 903
Hsp92I	GRCGYC	1 cut(s)	193
Hsp92II	CATG	4 cut(s)	70, 395, 404, 421
HspAI	GCGC	1 cut(s)	330
Kpn2I	TCCGGA	1 cut(s)	629
KroI	GCCGGC	1 cut(s)	148
KroNI	GCCGGC	1 cut(s)	150
Kzo9I	GATC	3 cut(s)	72, 229, 839
LmnI	GCTCC	5 cut(s)	223, 381, 472, 580, 632
Lsp1109I	GCAGC	2 cut(s)	360, 399
LweI	GCATC	1 cut(s)	563
MaeI	CTAG	1 cut(s)	489
MaeII	ACGT	2 cut(s)	440, 903
MaeIII	GTNAC	2 cut(s)	785, 816
MalI	GATC	3 cut(s)	74, 231, 841
MboI	GATC	3 cut(s)	72, 229, 839
MboII	GAAGA	6 cut(s)	97, 486, 518, 657, 665, 859
MflI	RGATCY	1 cut(s)	229
MhlI	GDGCHC	4 cut(s)	258, 351, 479, 629
MluCI	AATT	2 cut(s)	528, 547
MlyI	GAGTC	2 cut(s)	2, 29
MroI	TCCGGA	1 cut(s)	629
MroNI	GCCGGC	1 cut(s)	148
MspA1I	CMGCKG	1 cut(s)	542
MspI	CCGG	9 cut(s)	57, 149, 222, 619, 630, 635, 716, 798, 821
MspR9I	CCNGG	4 cut(s)	222, 223, 620, 636
Mva1269I	GAATGC	1 cut(s)	332
MvnI	CGCG	3 cut(s)	180, 332, 832
MwoI	GCNNNNNNNGC	3 cut(s)	201, 253, 624
NaeI	GCCGGC	1 cut(s)	150
NciI	CCSGG	4 cut(s)	222, 223, 620, 636
NcoI	CCATGG	1 cut(s)	391
NdeII	GATC	3 cut(s)	72, 229, 839
NgoMIV	GCCGGC	1 cut(s)	148
NlaIII	CATG	4 cut(s)	70, 395, 404, 421
NlaIV	GGNNCC	3 cut(s)	231, 576, 611
NmuCI	GTSAC	1 cut(s)	816
NspI	RCATGY	1 cut(s)	421
PagI	TCATGA	1 cut(s)	66
PciI	ACATGT	1 cut(s)	417
PcsI	WCGNNNNNNNCGW	2 cut(s)	357, 411
PctI	GAATGC	1 cut(s)	332
PdiI	GCCGGC	1 cut(s)	150
PfeI	GAWTC	1 cut(s)	396
PflFI	GACNNNGTC	1 cut(s)	195
PinAI	ACCGGT	1 cut(s)	820
PkrI	GCNGC	6 cut(s)	182, 203, 206, 241, 375, 389
PleI	GAGTC	2 cut(s)	2, 29
PmaCI	CACGTG	1 cut(s)	441
PmlI	CACGTG	1 cut(s)	441
PpsI	GAGTC	2 cut(s)	2, 29
Ppu21I	YACGTR	1 cut(s)	441
PpuMI	RGGWCCY	1 cut(s)	276
PscI	ACATGT	1 cut(s)	417
Psp124BI	GAGCTC	3 cut(s)	351, 479, 629
Psp5II	RGGWCCY	1 cut(s)	276
PspCI	CACGTG	1 cut(s)	441
PspFI	CCCAGC	1 cut(s)	674
PspN4I	GGNNCC	3 cut(s)	231, 576, 611
PspPI	GGNCC	3 cut(s)	276, 632, 659
PspPPI	RGGWCCY	1 cut(s)	276
PstI	CTGCAG	1 cut(s)	887
PsuI	RGATCY	1 cut(s)	229
PsyI	GACNNNGTC	1 cut(s)	195
PvuII	CAGCTG	1 cut(s)	542
RsaI	GTAC	1 cut(s)	140
RsaNI	GTAC	1 cut(s)	139
Rsr2I	CGGWCCG	1 cut(s)	632
RsrII	CGGWCCG	1 cut(s)	632
SacI	GAGCTC	3 cut(s)	351, 479, 629
SatI	GCNGC	6 cut(s)	181, 202, 205, 240, 374, 388
Sau3AI	GATC	3 cut(s)	72, 229, 839
Sau96I	GGNCC	3 cut(s)	276, 632, 659
SchI	GAGTC	2 cut(s)	2, 29
ScrFI	CCNGG	4 cut(s)	222, 223, 620, 636
SduI	GDGCHC	4 cut(s)	258, 351, 479, 629
SfaNI	GCATC	1 cut(s)	563
SfcI	CTRYAG	2 cut(s)	451, 883
SgrAI	CRCCGGYG	1 cut(s)	820
SinI	GGWCC	2 cut(s)	276, 632
SmaI	CCCGGG	1 cut(s)	223
Sse9I	AATT	2 cut(s)	528, 547
SsiI	CCGC	8 cut(s)	12, 180, 202, 205, 240, 247, 332, 832
SspMI	CTAG	1 cut(s)	489
SstI	GAGCTC	3 cut(s)	351, 479, 629
StyD4I	CCNGG	4 cut(s)	220, 221, 618, 634
StyI	CCWWGG	1 cut(s)	391
TaaI	ACNGT	1 cut(s)	320
TaiI	ACGT	2 cut(s)	443, 906
TaqI	TCGA	7 cut(s)	22, 38, 258, 324, 405, 414, 566
TasI	AATT	2 cut(s)	528, 547
TauI	GCSGC	4 cut(s)	183, 204, 207, 242
TfiI	GAWTC	1 cut(s)	396
TscAI	CASTG	3 cut(s)	33, 249, 849
TseFI	GTSAC	1 cut(s)	816
TseI	GCWGC	2 cut(s)	373, 387
Tsp45I	GTSAC	1 cut(s)	816
TspMI	CCCGGG	1 cut(s)	221
TspRI	CASTG	3 cut(s)	33, 249, 849
Tth111I	GACNNNGTC	1 cut(s)	195
VpaK11BI	GGWCC	2 cut(s)	276, 632
XapI	RAATTY	2 cut(s)	528, 547
XceI	RCATGY	1 cut(s)	421
XmaI	CCCGGG	1 cut(s)	221
XspI	CTAG	1 cut(s)	489

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.