Rh2DG483700

RING-H2 finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0011230
Physical Location & Seq
Reverse (-)
69944895 .. 69946939
2045 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG483700.1

Sequence Viewer

Length: 918 bp
ATGGGAGACGCAGCTATCGGCCTCGATGACTCCCAGGCCGTACAACTCACTGGAAAGATCATGATGATCGCCGTACTAGTCCTCTTCTTAGTCGTCGTCTTCGTCCTCTGCCTCCACCTCTACGCCAAATGGTACTGGTGGAGCGTAGTGGAAGATCCCAACTCCTCCACCCGCCAAACCGGCCGCCGACGACGCCGTTTCGTATTCGCTCCGGGCCAGGAATCCGAACTACCAACCCTCCGGCGAGGGCTCGAGCCTTCAGTCCTTAAGTCTCTGCCTGTAGTGGTCTTCAGTCCTCAAGATTTCAAAGACGGGTTGGAATGCGCTGTTTGTCTCTCTGATCTTGTTCAAGGCGAGAAGGCCAGATTGCTGCCCAAATGTAACCATGGATTTCATGTAGAGTGCATTGACATGTGGTTTCAGTCACATTCAACATGCCCGCTTTGCCGGAGCGCCGTCGTGCCGGAGACCGTAGCGCGCTCTGATTCCTCTGTTTCTGAGCTTAGTGTTGAAATTCCATCGCCGGCTGAGACTTTGGCTTCTGGGTTCTCTTCAGAATCACCCAATTTCCCTACAAATGTGTTGTTCTGGGGGAACCAGACCCGGGTTAGCTCCGGGCCGGGTTCGGGTTTGGAGGAAGGGACTTCAGCTCAAGTGCCTTGTCCCTCTTCTTCTTCTGATACTAGAAGACCGGAGGGAATGTTGGTGATTGATATACCGAGTGAGAATCCAATGGCATCTTTAGCATCGCCTTCGCCTTCCGGCAATAACAGGTTTGCTGCCGCCGAGGAGGACTTGAAGTCTCCGGTGCCGACGAGGTTGAGGTCACTCAAGAGGTTATTGAGCCGAGATAGGAAGATAAGTCCTTGTAGTCCAAGCTCAGCTGTTGATGCTGAAGCAGGGAGGGCACAAAGTTAG

Protein Analysis

305

Amino Acids

33.05

Weight (kDa)

5.89

Isoelectric Point (pI)

71.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 107 - 150 1e-13 Ring finger domain
zf-RING_11 PF17123 107 - 136 2.4e-10 RING-like zinc finger
zf-C3HC4_2 PF13923 107 - 149 1.1e-06 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 108 - 149 9.9e-06 Zinc finger, C3HC4 type (RING finger)
zf-rbx1 PF12678 122 - 150 1.2e-07 RING-H2 zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011230)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G53820 AT1G72310
fragaria_vesca FvH4_6g35980
malus_domestica MD09G1172100.v1.1 MD17G1154800.v1.1
prunus_persica Prupe.3G030500_v2.0.a1
pyrus_communis pycom09g08970 pycom17g14760
rosa_chinensis RchiOBHm_Chr2g0148101
rosa_laevigata RLG00000020313
rosa_multiflora Rmu_co8467879.1_g000001 Rmu_sc0001516.1_g000036 Rmu_sc0032339.1_g000001
rosa_roxburghii Rroxscaffold_2G00099150
rosa_rugosa Rorug02G0403500
rosa_samantha Rh2AG461300 Rh2BG475100 Rh2CG448400 Rh2DG483700
rosa_wichuraiana Rw2G037660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 808
AccII CGCG 1 cut(s) 478
AciI CCGC 4 cut(s) 172, 184, 440, 783
AclWI GGATC 1 cut(s) 149
AcoI YGGCCR 1 cut(s) 181
AcsI RAATTY 1 cut(s) 513
AcuI CTGAAG 5 cut(s) 243, 274, 537, 630, 915
AcyI GRCGYC 1 cut(s) 193
AfaI GTAC 3 cut(s) 42, 75, 134
AfiI CCNNNNNNNGG 2 cut(s) 604, 626
AflII CTTAAG 1 cut(s) 266
AflIII ACRYGT 1 cut(s) 411
AgsI TTSAA 5 cut(s) 307, 350, 432, 512, 799
AhdI GACNNNNNGTC 1 cut(s) 799
AhlI ACTAGT 1 cut(s) 76
AjnI CCWGG 2 cut(s) 33, 216
AluBI AGCT 6 cut(s) 14, 502, 612, 650, 879, 884
AluI AGCT 6 cut(s) 14, 502, 612, 650, 879, 884
Alw26I GTCTC 5 cut(s) 276, 338, 461, 524, 807
AlwI GGATC 1 cut(s) 149
Ama87I CYCGRG 2 cut(s) 251, 603
AoxI GGCC 6 cut(s) 19, 36, 181, 214, 360, 617
ApeKI GCWGC 3 cut(s) 11, 370, 779
ApoI RAATTY 1 cut(s) 513
AspLEI GCGC 4 cut(s) 326, 455, 478, 480
AspS9I GGNCC 2 cut(s) 214, 617
AsuC2I CCSGG 5 cut(s) 213, 604, 605, 616, 621
AsuHPI GGTGA 2 cut(s) 552, 718
AvaI CYCGRG 2 cut(s) 251, 603
BaeGI GKGCMC 1 cut(s) 910
BanI GGYRCC 1 cut(s) 808
BanII GRGCYC 1 cut(s) 252
BbsI GAAGAC 3 cut(s) 91, 280, 694
BbvI GCAGC 3 cut(s) 23, 357, 766
BccI CCATC 1 cut(s) 526
BceAI ACGGC 4 cut(s) 23, 56, 180, 440
BcgI CGANNNNNNTGC 2 cut(s) 735, 769
BciT130I CCWGG 2 cut(s) 35, 218
BcnI CCSGG 5 cut(s) 213, 604, 605, 616, 621
BcoDI GTCTC 5 cut(s) 276, 338, 461, 524, 807
BcuI ACTAGT 1 cut(s) 76
BfaI CTAG 2 cut(s) 77, 684
BfmI CTRYAG 1 cut(s) 279
BfoI RGCGCY 1 cut(s) 456
BfrI CTTAAG 1 cut(s) 266
BglI GCCNNNNNGGC 1 cut(s) 180
BisI GCNGC 5 cut(s) 12, 184, 371, 780, 783
BlpI GCTNAGC 1 cut(s) 880
BlsI GCNGC 5 cut(s) 13, 185, 372, 781, 784
Bme1390I CCNGG 7 cut(s) 35, 213, 218, 604, 605, 616, 621
BmeRI GACNNNNNGTC 1 cut(s) 799
BmeT110I CYCGRG 2 cut(s) 251, 603
BmgT120I GGNCC 2 cut(s) 214, 617
BmiI GGNNCC 2 cut(s) 596, 810
BmrFI CCNGG 7 cut(s) 35, 213, 218, 604, 605, 616, 621
BmsI GCATC 3 cut(s) 746, 755, 880
BpiI GAAGAC 3 cut(s) 91, 280, 694
Bpu1102I GCTNAGC 1 cut(s) 880
BpuEI CTTGAG 3 cut(s) 282, 636, 815
BpuMI CCSGG 5 cut(s) 213, 604, 605, 616, 621
BsaHI GRCGYC 1 cut(s) 193
BsaI GGTCTC 1 cut(s) 461
BsaJI CCNNGG 4 cut(s) 33, 385, 603, 786
BsaWI WCCGGW 2 cut(s) 691, 805
BsaXI ACNNNNNCTCC 4 cut(s) 222, 252, 686, 716
Bsc4I CCNNNNNNNGG 2 cut(s) 604, 626
Bse118I RCCGGY 2 cut(s) 179, 523
Bse1I ACTGG 2 cut(s) 55, 140
BseBI CCWGG 2 cut(s) 35, 218
BseDI CCNNGG 4 cut(s) 33, 385, 603, 786
BseLI CCNNNNNNNGG 2 cut(s) 604, 626
BseMII CTCAG 3 cut(s) 489, 519, 894
BseNI ACTGG 2 cut(s) 55, 140
BsePI GCGCGC 1 cut(s) 476
BseRI GAGGAG 2 cut(s) 154, 803
BseSI GKGCMC 1 cut(s) 910
BseX3I CGGCCG 1 cut(s) 181
BseXI GCAGC 3 cut(s) 23, 357, 766
Bsh1236I CGCG 1 cut(s) 478
Bsh1285I CGRYCG 1 cut(s) 184
BshFI GGCC 6 cut(s) 21, 38, 183, 216, 362, 619
BshNI GGYRCC 1 cut(s) 808
BsiEI CGRYCG 1 cut(s) 184
BsiHKCI CYCGRG 2 cut(s) 251, 603
BslFI GGGAC 2 cut(s) 648, 655
BslI CCNNNNNNNGG 2 cut(s) 604, 626
BsmAI GTCTC 5 cut(s) 276, 338, 461, 524, 807
BsmFI GGGAC 2 cut(s) 648, 655
BsmI GAATGC 1 cut(s) 326
BsnI GGCC 6 cut(s) 21, 38, 183, 216, 362, 619
Bso31I GGTCTC 1 cut(s) 461
BsoBI CYCGRG 2 cut(s) 251, 603
Bsp1286I GDGCHC 2 cut(s) 252, 910
Bsp143I GATC 4 cut(s) 57, 66, 154, 340
Bsp1720I GCTNAGC 1 cut(s) 880
Bsp19I CCATGG 1 cut(s) 385
BspACI CCGC 4 cut(s) 172, 184, 440, 783
BspANI GGCC 6 cut(s) 21, 38, 183, 216, 362, 619
BspCNI CTCAG 3 cut(s) 490, 520, 893
BspFNI CGCG 1 cut(s) 478
BspHI TCATGA 1 cut(s) 60
BspLI GGNNCC 2 cut(s) 596, 810
BspPI GGATC 1 cut(s) 149
BspT107I GGYRCC 1 cut(s) 808
BspTI CTTAAG 1 cut(s) 266
BspTNI GGTCTC 1 cut(s) 461
BsrFI RCCGGY 2 cut(s) 179, 523
BsrI ACTGG 2 cut(s) 55, 140
BssAI RCCGGY 2 cut(s) 179, 523
BssECI CCNNGG 4 cut(s) 33, 385, 603, 786
BssHII GCGCGC 1 cut(s) 476
BssMI GATC 4 cut(s) 57, 66, 154, 340
BssNI GRCGYC 1 cut(s) 193
BssT1I CCWWGG 1 cut(s) 385
Bst2UI CCWGG 2 cut(s) 35, 218
Bst4CI ACNGT 1 cut(s) 472
Bst6I CTCTTC 3 cut(s) 89, 556, 673
BstACI GRCGYC 1 cut(s) 193
BstAFI CTTAAG 1 cut(s) 266
BstC8I GCNNGC 3 cut(s) 440, 478, 525
BstDEI CTNAG 5 cut(s) 88, 498, 503, 528, 880
BstDSI CCRYGG 1 cut(s) 385
BstFNI CGCG 1 cut(s) 478
BstH2I RGCGCY 1 cut(s) 456
BstHHI GCGC 4 cut(s) 326, 455, 478, 480
BstKTI GATC 4 cut(s) 60, 69, 157, 343
BstMAI GTCTC 5 cut(s) 276, 338, 461, 524, 807
BstMBI GATC 4 cut(s) 57, 66, 154, 340
BstMCI CGRYCG 1 cut(s) 184
BstMWI GCNNNNNNNGC 6 cut(s) 180, 192, 444, 743, 890, 905
BstNI CCWGG 2 cut(s) 35, 218
BstNSI RCATGY 2 cut(s) 415, 438
BstSCI CCNGG 7 cut(s) 33, 211, 216, 602, 603, 614, 619
BstSFI CTRYAG 1 cut(s) 279
BstSLI GKGCMC 1 cut(s) 910
BstUI CGCG 1 cut(s) 478
BstV1I GCAGC 3 cut(s) 23, 357, 766
BstV2I GAAGAC 3 cut(s) 91, 280, 694
BstX2I RGATCY 1 cut(s) 154
BstYI RGATCY 1 cut(s) 154
BstZI CGGCCG 1 cut(s) 181
BsuRI GGCC 6 cut(s) 21, 38, 183, 216, 362, 619
BtgI CCRYGG 1 cut(s) 385
BtgZI GCGATG 2 cut(s) 504, 732
BtsIMutI CAGTG 1 cut(s) 48
Cac8I GCNNGC 3 cut(s) 440, 478, 525
CciI TCATGA 1 cut(s) 60
CfoI GCGC 4 cut(s) 326, 455, 478, 480
Cfr10I RCCGGY 2 cut(s) 179, 523
Cfr13I GGNCC 2 cut(s) 214, 617
Cfr9I CCCGGG 1 cut(s) 603
CseI GACGC 2 cut(s) 17, 201
Csp6I GTAC 3 cut(s) 41, 74, 133
CviAII CATG 5 cut(s) 61, 386, 395, 412, 435
CviQI GTAC 3 cut(s) 41, 74, 133
DdeI CTNAG 5 cut(s) 88, 498, 503, 528, 880
DpnI GATC 4 cut(s) 59, 68, 156, 342
DpnII GATC 4 cut(s) 57, 66, 154, 340
DriI GACNNNNNGTC 1 cut(s) 799
EaeI YGGCCR 1 cut(s) 181
EagI CGGCCG 1 cut(s) 181
Eam1104I CTCTTC 3 cut(s) 89, 556, 673
Eam1105I GACNNNNNGTC 1 cut(s) 799
EarI CTCTTC 3 cut(s) 89, 556, 673
EclXI CGGCCG 1 cut(s) 181
Eco130I CCWWGG 1 cut(s) 385
Eco24I GRGCYC 1 cut(s) 252
Eco31I GGTCTC 1 cut(s) 461
Eco52I CGGCCG 1 cut(s) 181
Eco57I CTGAAG 5 cut(s) 243, 274, 537, 630, 915
Eco88I CYCGRG 2 cut(s) 251, 603
EcoRII CCWGG 2 cut(s) 33, 216
EcoT14I CCWWGG 1 cut(s) 385
EcoT38I GRGCYC 1 cut(s) 252
ErhI CCWWGG 1 cut(s) 385
FaeI CATG 5 cut(s) 64, 389, 398, 415, 438
FaiI YATR 6 cut(s) 62, 387, 396, 413, 436, 716
FaqI GGGAC 2 cut(s) 648, 655
FatI CATG 5 cut(s) 60, 385, 394, 411, 434
FauI CCCGC 2 cut(s) 179, 447
Fnu4HI GCNGC 5 cut(s) 12, 184, 371, 780, 783
FriOI GRGCYC 1 cut(s) 252
Fsp4HI GCNGC 5 cut(s) 12, 184, 371, 780, 783
FspBI CTAG 2 cut(s) 77, 684
GlaI GCGC 4 cut(s) 325, 454, 477, 479
GluI GCNGC 5 cut(s) 12, 184, 371, 780, 783
HaeII RGCGCY 1 cut(s) 456
HaeIII GGCC 6 cut(s) 21, 38, 183, 216, 362, 619
HgaI GACGC 2 cut(s) 17, 201
HhaI GCGC 4 cut(s) 326, 455, 478, 480
Hin1I GRCGYC 1 cut(s) 193
Hin1II CATG 5 cut(s) 64, 389, 398, 415, 438
Hin6I GCGC 4 cut(s) 324, 453, 476, 478
HinP1I GCGC 4 cut(s) 324, 453, 476, 478
HinfI GANTC 5 cut(s) 29, 221, 485, 557, 727
HphI GGTGA 2 cut(s) 552, 718
Hpy188I TCNGA 6 cut(s) 226, 340, 484, 499, 556, 679
Hpy188III TCNNGA 3 cut(s) 61, 299, 832
Hpy99I CGWCG 5 cut(s) 98, 192, 195, 461, 817
HpyAV CCTTC 5 cut(s) 267, 352, 632, 762, 768
HpyCH4III ACNGT 1 cut(s) 472
HpyCH4V TGCA 1 cut(s) 405
HpyF10VI GCNNNNNNNGC 6 cut(s) 180, 192, 444, 743, 890, 905
HpyF3I CTNAG 5 cut(s) 88, 498, 503, 528, 880
Hsp92I GRCGYC 1 cut(s) 193
Hsp92II CATG 5 cut(s) 64, 389, 398, 415, 438
HspAI GCGC 4 cut(s) 324, 453, 476, 478
KroI GCCGGC 1 cut(s) 523
KroNI GCCGGC 1 cut(s) 525
Kzo9I GATC 4 cut(s) 57, 66, 154, 340
LmnI GCTCC 4 cut(s) 141, 214, 450, 617
Lsp1109I GCAGC 3 cut(s) 23, 357, 766
LweI GCATC 3 cut(s) 746, 755, 880
MaeI CTAG 2 cut(s) 77, 684
MaeIII GTNAC 3 cut(s) 380, 423, 825
MalI GATC 4 cut(s) 59, 68, 156, 342
MboI GATC 4 cut(s) 57, 66, 154, 340
MflI RGATCY 1 cut(s) 154
MhlI GDGCHC 2 cut(s) 252, 910
MluCI AATT 2 cut(s) 513, 565
MlyI GAGTC 1 cut(s) 23
MmeI TCCRAC 1 cut(s) 297
MroNI GCCGGC 1 cut(s) 523
MseI TTAA 1 cut(s) 267
MslI CAYNNNNRTG 1 cut(s) 410
MspA1I CMGCKG 1 cut(s) 884
MspCI CTTAAG 1 cut(s) 266
MspR9I CCNGG 7 cut(s) 35, 213, 218, 604, 605, 616, 621
Mva1269I GAATGC 1 cut(s) 326
MvaI CCWGG 2 cut(s) 35, 218
MvnI CGCG 1 cut(s) 478
MwoI GCNNNNNNNGC 6 cut(s) 180, 192, 444, 743, 890, 905
NaeI GCCGGC 1 cut(s) 525
NciI CCSGG 5 cut(s) 213, 604, 605, 616, 621
NcoI CCATGG 1 cut(s) 385
NdeII GATC 4 cut(s) 57, 66, 154, 340
NgoMIV GCCGGC 1 cut(s) 523
NlaIII CATG 5 cut(s) 64, 389, 398, 415, 438
NlaIV GGNNCC 2 cut(s) 596, 810
NmeAIII GCCGAG 2 cut(s) 811, 872
NmuCI GTSAC 2 cut(s) 423, 825
NspI RCATGY 2 cut(s) 415, 438
PaeR7I CTCGAG 1 cut(s) 251
PagI TCATGA 1 cut(s) 60
PauI GCGCGC 1 cut(s) 476
PciI ACATGT 1 cut(s) 411
PcsI WCGNNNNNNNCGW 1 cut(s) 99
PctI GAATGC 1 cut(s) 326
PdiI GCCGGC 1 cut(s) 525
PfeI GAWTC 4 cut(s) 221, 485, 557, 727
PkrI GCNGC 5 cut(s) 13, 185, 372, 781, 784
PleI GAGTC 1 cut(s) 23
PpsI GAGTC 1 cut(s) 23
PscI ACATGT 1 cut(s) 411
Psp6I CCWGG 2 cut(s) 33, 216
PspGI CCWGG 2 cut(s) 33, 216
PspN4I GGNNCC 2 cut(s) 596, 810
PspPI GGNCC 2 cut(s) 214, 617
PspXI VCTCGAGB 1 cut(s) 251
PsuI RGATCY 1 cut(s) 154
PteI GCGCGC 1 cut(s) 476
PvuII CAGCTG 1 cut(s) 884
RsaI GTAC 3 cut(s) 42, 75, 134
RsaNI GTAC 3 cut(s) 41, 74, 133
RseI CAYNNNNRTG 1 cut(s) 410
SaqAI TTAA 1 cut(s) 267
SatI GCNGC 5 cut(s) 12, 184, 371, 780, 783
Sau3AI GATC 4 cut(s) 57, 66, 154, 340
Sau96I GGNCC 2 cut(s) 214, 617
SchI GAGTC 1 cut(s) 23
ScrFI CCNGG 7 cut(s) 35, 213, 218, 604, 605, 616, 621
SduI GDGCHC 2 cut(s) 252, 910
SfaNI GCATC 3 cut(s) 746, 755, 880
SfcI CTRYAG 1 cut(s) 279
Sfr274I CTCGAG 1 cut(s) 251
SlaI CTCGAG 1 cut(s) 251
SmaI CCCGGG 1 cut(s) 605
SmiMI CAYNNNNRTG 1 cut(s) 410
SmlI CTYRAG 5 cut(s) 251, 266, 297, 651, 830
SmoI CTYRAG 5 cut(s) 251, 266, 297, 651, 830
SpeI ACTAGT 1 cut(s) 76
Sse9I AATT 2 cut(s) 513, 565
SsiI CCGC 4 cut(s) 172, 184, 440, 783
SspMI CTAG 2 cut(s) 77, 684
StyD4I CCNGG 7 cut(s) 33, 211, 216, 602, 603, 614, 619
StyI CCWWGG 1 cut(s) 385
TaaI ACNGT 1 cut(s) 472
TaqI TCGA 2 cut(s) 24, 252
TasI AATT 2 cut(s) 513, 565
TauI GCSGC 2 cut(s) 186, 785
TfiI GAWTC 4 cut(s) 221, 485, 557, 727
Tru1I TTAA 1 cut(s) 267
Tru9I TTAA 1 cut(s) 267
TscAI CASTG 1 cut(s) 55
TseFI GTSAC 2 cut(s) 423, 825
TseI GCWGC 3 cut(s) 11, 370, 779
Tsp45I GTSAC 2 cut(s) 423, 825
TspDTI ATGAA 1 cut(s) 383
TspMI CCCGGG 1 cut(s) 603
TspRI CASTG 1 cut(s) 55
Vha464I CTTAAG 1 cut(s) 266
XapI RAATTY 1 cut(s) 513
XceI RCATGY 2 cut(s) 415, 438
XhoI CTCGAG 1 cut(s) 251
XmaI CCCGGG 1 cut(s) 603
XspI CTAG 2 cut(s) 77, 684
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.