RLG00000020313

RING-H2 finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0011230
Physical Location & Seq
Reverse (-)
63567573 .. 63569167
1595 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000020313

Sequence Viewer

Length: 1176 bp
ATGAAAATTGAAGAGGAAGAGACCGAAAAACTGAGAAAAAGTCATAAAAGAGGTCAAGAGAAAGGAAAGCGTCGAGAAGGGACACGTGGAGCGGGAACAACCGATTTGGGTAGTGAAATCTACCCAACTCCTCATCTGATCTCTTCTCATTTCTCCACAAAAGCCTCAAACTTGATTTGGGTATCAAACTCCTCCCTCAACTCTCATCTGGGCTCTCCTCATTCTCCTGATTCCGAGCTAAAGCTCTCACCTTTATCAATGGGAGACACAGCTCTCGGCCTCGATGACTCACAGGCCGTACAACTCACCGGAAAGATCATGATGATCGCCGTACTAGTCCTCTTCTTAGTCGTCGTCTTCGTCCTCTGCCTCCACCTCTACGCCAAATGGTACTGGTGGAACGTAGTGGACGATCCCAACTCCTCCACCCGACAAACCGGCCGCCGACGACGCCGTTTCGTCTTCGCTCCGGGCCAGGAATCCGAACTACCAACCCTCCGGCGAGGGCTCGAGCCTTCAGTCGTAAAGTCTTTGCCTGTAGTGGTCTTCAGTCCTCAAGATTTCAAAGATGGGTTGGAATGCGCTGTTTGTCTCTCTGATCTTGTTGAAGGTGAAAAGGCCAGATTGCTTCCCAAATGTAACCATGGATTTCATGTTGAGTGCATTGACATGTGGTTTCAGTCACATTCAACATGCCCGCTTTGCCGGAACGCCGTCGTGCCGGAGACCGTAGCGCGCTCTGATTCCTCTGTTTCTGAGCTCAGTGTTGAAATTCCATCGCCGGCTGAGACTTTGGCTTCTGGGTTCTCTTCAGAATCACCCAATTTCCCTACAAATGTGTTGTTCTGGGGGAACCAGACCCGGGTTAGCTCCGGGCCGGGTTCGGGTTTGGAGGAAGGGACTTCAGCTCAAGTGCCTTGTCCCTCTTCTTCTTCTGATACTAGAAGACCGGAGGGAATGTTGGTGATTGATATACCGAGTGAGAATCCAATGGCATCTTTAGCATCGCCTTCGCCTTCCGGCAATAACAGGTTTGCTGCCGCCGAGGAGGACTTGAAGTCTCCGGTGCCGACGAGGTTGAGGTCACTCAAGAGGTTATTGAGCCGAGATAGGAGGATAAGTCCTTGTAGTCCAAGCTCAGCTGTTGATGCTGAAGCAGGGAGAGCACAAAGTTAG

Protein Analysis

392

Amino Acids

42.63

Weight (kDa)

6.53

Isoelectric Point (pI)

70.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 193 - 236 7.2e-14 Ring finger domain
zf-RING_11 PF17123 193 - 222 2.5e-10 RING-like zinc finger
zf-C3HC4_2 PF13923 193 - 235 1e-06 Zinc finger, C3HC4 type (RING finger)
zf-rbx1 PF12678 208 - 236 9.4e-08 RING-H2 zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011230)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G53820 AT1G72310
fragaria_vesca FvH4_6g35980
malus_domestica MD09G1172100.v1.1 MD17G1154800.v1.1
prunus_persica Prupe.3G030500_v2.0.a1
pyrus_communis pycom09g08970 pycom17g14760
rosa_chinensis RchiOBHm_Chr2g0148101
rosa_laevigata RLG00000020313
rosa_multiflora Rmu_co8467879.1_g000001 Rmu_sc0001516.1_g000036 Rmu_sc0032339.1_g000001
rosa_roxburghii Rroxscaffold_2G00099150
rosa_rugosa Rorug02G0403500
rosa_samantha Rh2AG461300 Rh2BG475100 Rh2CG448400 Rh2DG483700
rosa_wichuraiana Rw2G037660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1066
AccBSI CCGCTC 1 cut(s) 92
AccII CGCG 1 cut(s) 736
AciI CCGC 4 cut(s) 92, 442, 698, 1041
AclWI GGATC 1 cut(s) 407
AcoI YGGCCR 1 cut(s) 439
AcsI RAATTY 1 cut(s) 771
AcuI CTGAAG 5 cut(s) 501, 532, 795, 888, 1173
AcvI CACGTG 1 cut(s) 86
AcyI GRCGYC 1 cut(s) 451
AfaI GTAC 3 cut(s) 300, 333, 392
AfiI CCNNNNNNNGG 2 cut(s) 862, 884
AflIII ACRYGT 2 cut(s) 83, 669
AgsI TTSAA 6 cut(s) 11, 565, 608, 690, 770, 1057
AhdI GACNNNNNGTC 1 cut(s) 1057
AhlI ACTAGT 1 cut(s) 334
AjnI CCWGG 1 cut(s) 474
AluBI AGCT 8 cut(s) 238, 244, 272, 760, 870, 908, 1137, 1142
AluI AGCT 8 cut(s) 238, 244, 272, 760, 870, 908, 1137, 1142
Alw21I GWGCWC 2 cut(s) 762, 1168
Alw26I GTCTC 6 cut(s) 14, 258, 596, 719, 782, 1065
AlwI GGATC 1 cut(s) 407
Ama87I CYCGRG 2 cut(s) 509, 861
AoxI GGCC 6 cut(s) 277, 294, 439, 472, 618, 875
ApeKI GCWGC 1 cut(s) 1037
ApoI RAATTY 1 cut(s) 771
AspLEI GCGC 3 cut(s) 584, 736, 738
AspS9I GGNCC 2 cut(s) 472, 875
AsuC2I CCSGG 5 cut(s) 471, 862, 863, 874, 879
AsuHPI GGTGA 5 cut(s) 240, 298, 623, 810, 976
AvaI CYCGRG 2 cut(s) 509, 861
BanI GGYRCC 1 cut(s) 1066
BanII GRGCYC 3 cut(s) 215, 510, 762
BbrPI CACGTG 1 cut(s) 86
BbsI GAAGAC 4 cut(s) 349, 454, 538, 952
Bbv12I GWGCWC 2 cut(s) 762, 1168
BbvI GCAGC 1 cut(s) 1024
BccI CCATC 2 cut(s) 563, 784
BceAI ACGGC 4 cut(s) 281, 314, 438, 698
BcgI CGANNNNNNTGC 2 cut(s) 993, 1027
BciT130I CCWGG 1 cut(s) 476
BcnI CCSGG 5 cut(s) 471, 862, 863, 874, 879
BcoDI GTCTC 6 cut(s) 14, 258, 596, 719, 782, 1065
BcuI ACTAGT 1 cut(s) 334
BfaI CTAG 2 cut(s) 335, 942
BfmI CTRYAG 1 cut(s) 537
BisI GCNGC 3 cut(s) 442, 1038, 1041
BlpI GCTNAGC 1 cut(s) 1138
BlsI GCNGC 3 cut(s) 443, 1039, 1042
Bme1390I CCNGG 6 cut(s) 471, 476, 862, 863, 874, 879
BmeRI GACNNNNNGTC 1 cut(s) 1057
BmeT110I CYCGRG 2 cut(s) 509, 861
BmgT120I GGNCC 2 cut(s) 472, 875
BmiI GGNNCC 2 cut(s) 854, 1068
BmrFI CCNGG 6 cut(s) 471, 476, 862, 863, 874, 879
BmsI GCATC 3 cut(s) 1004, 1013, 1138
BpiI GAAGAC 4 cut(s) 349, 454, 538, 952
Bpu1102I GCTNAGC 1 cut(s) 1138
BpuEI CTTGAG 3 cut(s) 540, 894, 1073
BpuMI CCSGG 5 cut(s) 471, 862, 863, 874, 879
BsaAI YACGTR 1 cut(s) 86
BsaHI GRCGYC 1 cut(s) 451
BsaI GGTCTC 2 cut(s) 14, 719
BsaJI CCNNGG 3 cut(s) 643, 861, 1044
BsaWI WCCGGW 3 cut(s) 308, 949, 1063
BsaXI ACNNNNNCTCC 6 cut(s) 480, 510, 944, 974, 1105, 1135
Bsc4I CCNNNNNNNGG 2 cut(s) 862, 884
Bse118I RCCGGY 2 cut(s) 437, 781
Bse1I ACTGG 1 cut(s) 398
BseBI CCWGG 1 cut(s) 476
BseDI CCNNGG 3 cut(s) 643, 861, 1044
BseLI CCNNNNNNNGG 2 cut(s) 862, 884
BseMII CTCAG 5 cut(s) 23, 747, 775, 777, 1152
BseNI ACTGG 1 cut(s) 398
BsePI GCGCGC 1 cut(s) 734
BseRI GAGGAG 5 cut(s) 120, 181, 207, 412, 1061
BseX3I CGGCCG 1 cut(s) 439
BseXI GCAGC 1 cut(s) 1024
Bsh1236I CGCG 1 cut(s) 736
Bsh1285I CGRYCG 1 cut(s) 442
BshFI GGCC 6 cut(s) 279, 296, 441, 474, 620, 877
BshNI GGYRCC 1 cut(s) 1066
BsiEI CGRYCG 1 cut(s) 442
BsiHKAI GWGCWC 2 cut(s) 762, 1168
BsiHKCI CYCGRG 2 cut(s) 509, 861
BslFI GGGAC 3 cut(s) 94, 906, 913
BslI CCNNNNNNNGG 2 cut(s) 862, 884
BsmAI GTCTC 6 cut(s) 14, 258, 596, 719, 782, 1065
BsmFI GGGAC 3 cut(s) 94, 906, 913
BsmI GAATGC 1 cut(s) 584
BsnI GGCC 6 cut(s) 279, 296, 441, 474, 620, 877
Bso31I GGTCTC 2 cut(s) 14, 719
BsoBI CYCGRG 2 cut(s) 509, 861
Bsp1286I GDGCHC 4 cut(s) 215, 510, 762, 1168
Bsp143I GATC 5 cut(s) 138, 315, 324, 412, 598
Bsp1720I GCTNAGC 1 cut(s) 1138
Bsp19I CCATGG 1 cut(s) 643
BspACI CCGC 4 cut(s) 92, 442, 698, 1041
BspANI GGCC 6 cut(s) 279, 296, 441, 474, 620, 877
BspCNI CTCAG 5 cut(s) 24, 748, 774, 778, 1151
BspFNI CGCG 1 cut(s) 736
BspHI TCATGA 1 cut(s) 318
BspLI GGNNCC 2 cut(s) 854, 1068
BspPI GGATC 1 cut(s) 407
BspT107I GGYRCC 1 cut(s) 1066
BspTNI GGTCTC 2 cut(s) 14, 719
BsrBI CCGCTC 1 cut(s) 92
BsrFI RCCGGY 2 cut(s) 437, 781
BsrI ACTGG 1 cut(s) 398
BssAI RCCGGY 2 cut(s) 437, 781
BssECI CCNNGG 3 cut(s) 643, 861, 1044
BssHII GCGCGC 1 cut(s) 734
BssMI GATC 5 cut(s) 138, 315, 324, 412, 598
BssNI GRCGYC 1 cut(s) 451
BssT1I CCWWGG 1 cut(s) 643
Bst2UI CCWGG 1 cut(s) 476
Bst4CI ACNGT 1 cut(s) 730
Bst6I CTCTTC 6 cut(s) 6, 12, 148, 347, 814, 931
BstACI GRCGYC 1 cut(s) 451
BstBAI YACGTR 1 cut(s) 86
BstC8I GCNNGC 3 cut(s) 698, 736, 783
BstDEI CTNAG 6 cut(s) 32, 346, 756, 761, 786, 1138
BstDSI CCRYGG 1 cut(s) 643
BstFNI CGCG 1 cut(s) 736
BstHHI GCGC 3 cut(s) 584, 736, 738
BstKTI GATC 5 cut(s) 141, 318, 327, 415, 601
BstMAI GTCTC 6 cut(s) 14, 258, 596, 719, 782, 1065
BstMBI GATC 5 cut(s) 138, 315, 324, 412, 598
BstMCI CGRYCG 1 cut(s) 442
BstMWI GCNNNNNNNGC 5 cut(s) 450, 702, 1001, 1148, 1163
BstNI CCWGG 1 cut(s) 476
BstNSI RCATGY 2 cut(s) 673, 696
BstSCI CCNGG 6 cut(s) 469, 474, 860, 861, 872, 877
BstSFI CTRYAG 1 cut(s) 537
BstUI CGCG 1 cut(s) 736
BstV1I GCAGC 1 cut(s) 1024
BstV2I GAAGAC 4 cut(s) 349, 454, 538, 952
BstZI CGGCCG 1 cut(s) 439
BsuRI GGCC 6 cut(s) 279, 296, 441, 474, 620, 877
BtgI CCRYGG 1 cut(s) 643
BtgZI GCGATG 2 cut(s) 762, 990
BtsIMutI CAGTG 1 cut(s) 769
Cac8I GCNNGC 3 cut(s) 698, 736, 783
CciI TCATGA 1 cut(s) 318
CfoI GCGC 3 cut(s) 584, 736, 738
Cfr10I RCCGGY 2 cut(s) 437, 781
Cfr13I GGNCC 2 cut(s) 472, 875
Cfr9I CCCGGG 1 cut(s) 861
CseI GACGC 2 cut(s) 59, 459
Csp6I GTAC 3 cut(s) 299, 332, 391
CviAII CATG 5 cut(s) 319, 644, 653, 670, 693
CviQI GTAC 3 cut(s) 299, 332, 391
DdeI CTNAG 6 cut(s) 32, 346, 756, 761, 786, 1138
DpnI GATC 5 cut(s) 140, 317, 326, 414, 600
DpnII GATC 5 cut(s) 138, 315, 324, 412, 598
DriI GACNNNNNGTC 1 cut(s) 1057
EaeI YGGCCR 1 cut(s) 439
EagI CGGCCG 1 cut(s) 439
Eam1104I CTCTTC 6 cut(s) 6, 12, 148, 347, 814, 931
Eam1105I GACNNNNNGTC 1 cut(s) 1057
EarI CTCTTC 6 cut(s) 6, 12, 148, 347, 814, 931
Ecl136II GAGCTC 1 cut(s) 760
EclXI CGGCCG 1 cut(s) 439
Eco130I CCWWGG 1 cut(s) 643
Eco24I GRGCYC 3 cut(s) 215, 510, 762
Eco31I GGTCTC 2 cut(s) 14, 719
Eco52I CGGCCG 1 cut(s) 439
Eco53kI GAGCTC 1 cut(s) 760
Eco57I CTGAAG 5 cut(s) 501, 532, 795, 888, 1173
Eco72I CACGTG 1 cut(s) 86
Eco88I CYCGRG 2 cut(s) 509, 861
EcoICRI GAGCTC 1 cut(s) 760
EcoRII CCWGG 1 cut(s) 474
EcoT14I CCWWGG 1 cut(s) 643
EcoT38I GRGCYC 3 cut(s) 215, 510, 762
ErhI CCWWGG 1 cut(s) 643
FaeI CATG 5 cut(s) 322, 647, 656, 673, 696
FaiI YATR 7 cut(s) 45, 320, 645, 654, 671, 694, 974
FaqI GGGAC 3 cut(s) 94, 906, 913
FatI CATG 5 cut(s) 318, 643, 652, 669, 692
FauI CCCGC 2 cut(s) 85, 705
Fnu4HI GCNGC 3 cut(s) 442, 1038, 1041
FriOI GRGCYC 3 cut(s) 215, 510, 762
Fsp4HI GCNGC 3 cut(s) 442, 1038, 1041
FspBI CTAG 2 cut(s) 335, 942
GlaI GCGC 3 cut(s) 583, 735, 737
GluI GCNGC 3 cut(s) 442, 1038, 1041
HaeIII GGCC 6 cut(s) 279, 296, 441, 474, 620, 877
HgaI GACGC 2 cut(s) 59, 459
HhaI GCGC 3 cut(s) 584, 736, 738
Hin1I GRCGYC 1 cut(s) 451
Hin1II CATG 5 cut(s) 322, 647, 656, 673, 696
Hin6I GCGC 3 cut(s) 582, 734, 736
HinP1I GCGC 3 cut(s) 582, 734, 736
HinfI GANTC 6 cut(s) 230, 287, 479, 743, 815, 985
HphI GGTGA 5 cut(s) 240, 298, 623, 810, 976
Hpy166II GTNNAC 1 cut(s) 409
Hpy188I TCNGA 8 cut(s) 138, 235, 484, 598, 742, 757, 814, 937
Hpy188III TCNNGA 6 cut(s) 56, 74, 227, 319, 557, 1090
Hpy8I GTNNAC 1 cut(s) 409
Hpy99I CGWCG 6 cut(s) 75, 356, 450, 453, 719, 1075
HpyAV CCTTC 6 cut(s) 71, 525, 602, 890, 1020, 1026
HpyCH4III ACNGT 1 cut(s) 730
HpyCH4IV ACGT 2 cut(s) 85, 402
HpyCH4V TGCA 1 cut(s) 663
HpyF10VI GCNNNNNNNGC 5 cut(s) 450, 702, 1001, 1148, 1163
HpyF3I CTNAG 6 cut(s) 32, 346, 756, 761, 786, 1138
HpySE526I ACGT 2 cut(s) 85, 402
Hsp92I GRCGYC 1 cut(s) 451
Hsp92II CATG 5 cut(s) 322, 647, 656, 673, 696
HspAI GCGC 3 cut(s) 582, 734, 736
KroI GCCGGC 1 cut(s) 781
KroNI GCCGGC 1 cut(s) 783
Kzo9I GATC 5 cut(s) 138, 315, 324, 412, 598
LmnI GCTCC 3 cut(s) 89, 472, 875
Lsp1109I GCAGC 1 cut(s) 1024
LweI GCATC 3 cut(s) 1004, 1013, 1138
MaeI CTAG 2 cut(s) 335, 942
MaeII ACGT 2 cut(s) 85, 402
MaeIII GTNAC 3 cut(s) 638, 681, 1083
MalI GATC 5 cut(s) 140, 317, 326, 414, 600
MbiI CCGCTC 1 cut(s) 92
MboI GATC 5 cut(s) 138, 315, 324, 412, 598
MhlI GDGCHC 4 cut(s) 215, 510, 762, 1168
MluCI AATT 3 cut(s) 6, 771, 823
MlyI GAGTC 1 cut(s) 281
MmeI TCCRAC 1 cut(s) 555
MroNI GCCGGC 1 cut(s) 781
MslI CAYNNNNRTG 1 cut(s) 668
MspA1I CMGCKG 1 cut(s) 1142
MspR9I CCNGG 6 cut(s) 471, 476, 862, 863, 874, 879
Mva1269I GAATGC 1 cut(s) 584
MvaI CCWGG 1 cut(s) 476
MvnI CGCG 1 cut(s) 736
MwoI GCNNNNNNNGC 5 cut(s) 450, 702, 1001, 1148, 1163
NaeI GCCGGC 1 cut(s) 783
NciI CCSGG 5 cut(s) 471, 862, 863, 874, 879
NcoI CCATGG 1 cut(s) 643
NdeII GATC 5 cut(s) 138, 315, 324, 412, 598
NgoMIV GCCGGC 1 cut(s) 781
NlaIII CATG 5 cut(s) 322, 647, 656, 673, 696
NlaIV GGNNCC 2 cut(s) 854, 1068
NmeAIII GCCGAG 3 cut(s) 255, 1069, 1130
NmuCI GTSAC 2 cut(s) 681, 1083
NspI RCATGY 2 cut(s) 673, 696
PaeR7I CTCGAG 1 cut(s) 509
PagI TCATGA 1 cut(s) 318
PauI GCGCGC 1 cut(s) 734
PciI ACATGT 1 cut(s) 669
PcsI WCGNNNNNNNCGW 2 cut(s) 357, 408
PctI GAATGC 1 cut(s) 584
PdiI GCCGGC 1 cut(s) 783
PfeI GAWTC 5 cut(s) 230, 479, 743, 815, 985
PkrI GCNGC 3 cut(s) 443, 1039, 1042
PleI GAGTC 1 cut(s) 281
PmaCI CACGTG 1 cut(s) 86
PmlI CACGTG 1 cut(s) 86
PpsI GAGTC 1 cut(s) 281
Ppu21I YACGTR 1 cut(s) 86
PscI ACATGT 1 cut(s) 669
Psp124BI GAGCTC 1 cut(s) 762
Psp6I CCWGG 1 cut(s) 474
PspCI CACGTG 1 cut(s) 86
PspGI CCWGG 1 cut(s) 474
PspN4I GGNNCC 2 cut(s) 854, 1068
PspPI GGNCC 2 cut(s) 472, 875
PspXI VCTCGAGB 1 cut(s) 509
PteI GCGCGC 1 cut(s) 734
PvuII CAGCTG 1 cut(s) 1142
RsaI GTAC 3 cut(s) 300, 333, 392
RsaNI GTAC 3 cut(s) 299, 332, 391
RseI CAYNNNNRTG 1 cut(s) 668
SacI GAGCTC 1 cut(s) 762
SatI GCNGC 3 cut(s) 442, 1038, 1041
Sau3AI GATC 5 cut(s) 138, 315, 324, 412, 598
Sau96I GGNCC 2 cut(s) 472, 875
SchI GAGTC 1 cut(s) 281
ScrFI CCNGG 6 cut(s) 471, 476, 862, 863, 874, 879
SduI GDGCHC 4 cut(s) 215, 510, 762, 1168
SfaNI GCATC 3 cut(s) 1004, 1013, 1138
SfcI CTRYAG 1 cut(s) 537
Sfr274I CTCGAG 1 cut(s) 509
SlaI CTCGAG 1 cut(s) 509
SmaI CCCGGG 1 cut(s) 863
SmiMI CAYNNNNRTG 1 cut(s) 668
SmlI CTYRAG 4 cut(s) 509, 555, 909, 1088
SmoI CTYRAG 4 cut(s) 509, 555, 909, 1088
SpeI ACTAGT 1 cut(s) 334
Sse9I AATT 3 cut(s) 6, 771, 823
SsiI CCGC 4 cut(s) 92, 442, 698, 1041
SspMI CTAG 2 cut(s) 335, 942
SstI GAGCTC 1 cut(s) 762
StyD4I CCNGG 6 cut(s) 469, 474, 860, 861, 872, 877
StyI CCWWGG 1 cut(s) 643
TaaI ACNGT 1 cut(s) 730
TaiI ACGT 2 cut(s) 88, 405
TaqI TCGA 3 cut(s) 73, 282, 510
TaqII GACCGA 1 cut(s) 38
TasI AATT 3 cut(s) 6, 771, 823
TauI GCSGC 2 cut(s) 444, 1043
TfiI GAWTC 5 cut(s) 230, 479, 743, 815, 985
TscAI CASTG 1 cut(s) 769
TseFI GTSAC 2 cut(s) 681, 1083
TseI GCWGC 1 cut(s) 1037
Tsp45I GTSAC 2 cut(s) 681, 1083
TspDTI ATGAA 2 cut(s) 17, 641
TspMI CCCGGG 1 cut(s) 861
TspRI CASTG 1 cut(s) 769
XapI RAATTY 1 cut(s) 771
XceI RCATGY 2 cut(s) 673, 696
XhoI CTCGAG 1 cut(s) 509
XmaI CCCGGG 1 cut(s) 861
XspI CTAG 2 cut(s) 335, 942
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.