pycom17g15400

pyrophosphate-energized membrane proton pump

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0005820
Physical Location & Seq
Forward (+)
13415634 .. 13418964
3331 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1422 bp
ATGATTCTTGGGGGAACGATGGCTCAACGTTGTAAAATCGAAGATGCATCTGGTTTCATCTTGTTTCCTCTGGTTGTTCACTCTTTTGATCTGGTGATTTCATCAGTTGGAATATTCTCAATTAGAGGTACACGTGATTCCAGTGGAAAGGCTTCTTCCGAGGATCCAATGAAAATTCTACAGAGAGGATATTCTGTTACAATACTCTTGGCTGTTCTGACATTTGGTGCGTCTACGCGATGGCTGCTTTATACTGAGCAAGCACCATCTGCGTGGTTTAACTTTGCCTTGTGTGGGCTAGTTGGCATCATCACAGCATATGTCTTCGTCTTTATCACCCAATACTATACGGACTACAAACACGAGCCTGTACGCACATTAGCTCTTGCAAGCTCCACAGGTCATGGGACTAATATAATTGCTGGAGTCAGTTTGGGTCTTGAATCAACTGCTCTTCCAGTTCTTGTCATTAGTGTATCTATTATCTCAGCTTACTGGCTGGGCCAGACCTCTGGCCTTATAGACGAACATGGAATTCCAACTGGTGGGCTGTTTGGCACAGCTGTAGCAACAATGGGAATGCTCAGTACTGCTGCATATGTTCTCACTATGGATATGTTTGGTCCAATAGCTGATAATGCTGGTGGAATTGTAGAGATGAGTCAGCAGCCTGAAAGTGTTCGGGAGATCGATCATGTTCTAGACGCAGTAGGAAACACTACAAAAGCTACCACTAAAGGATTTGCCATAGGATCTGCTGCACTTGCATCTTTTCTTCTGTTTAGTGCTTATATGGACGAGGTTGCTGCATTTGCACGTGAACCTTTCAAACAGGTTGATATTGCCATTCCAGAAGTTTTTGTTGGCGGATTGTTGGGCTCTATGCTGATATTTCTATTTAGCGCGTGGGCCTGTTCAGCAGTTGGGCGAACTGCACAAGAGGTTGTTAAGGAAGTAAGGCGGCAATTTATTGAGAGGCCTGGGATAATGGAGTACAAGGAGAAGCCAGATTATGCTCGTTGTGTTGCTATTGTAGCATCTGCATCTTTAAGGGAGATGATAAAACCTGGTGCCTTGGCTATTATTTCACCTATAGCTGTTGGTATTGTGTTCCGGATCTTTGGATACTACAATGGGCAACCTCTACTTGGTGCTAAAGTTGTGGCTTCCATGCTGATGTTTGCAACTGTTTCCGGTATTCTTATGGCACTTTTCCTCAACACAGCCGGAGGTGCCTGGGATAATGCAAAGAAGTACATTGAGACAGGGGTTCTCGGTGGCAAGGGAAGTGATTGCCATAAGGCTGCAGTTACAGGAGATACTGTGGGTGACCCATTTAAAGACACAGCCGGACCTTCGCTGCACGTTCTCATCAAAATGCTTGCAACTATAACGCTGGTTATGGCTCCGGTCTTTCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00190 map00190
Pfam Domains
Protein Families
H_PPase

Protein Analysis

474

Amino Acids

50.24

Weight (kDa)

6.01

Isoelectric Point (pI)

29.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
H_PPase PF03030 10 - 468 8.4e-162 Inorganic H+ pyrophosphatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 1070, 1232
AccB7I CCANNNNNTGG 1 cut(s) 545
AccI GTMKAC 1 cut(s) 233
AccII CGCG 2 cut(s) 238, 905
AccIII TCCGGA 1 cut(s) 1113
AciI CCGC 2 cut(s) 867, 961
AclI AACGTT 1 cut(s) 28
AclWI GGATC 4 cut(s) 158, 171, 760, 1124
AcsI RAATTY 2 cut(s) 174, 534
AcvI CACGTG 2 cut(s) 134, 818
AfaI GTAC 5 cut(s) 130, 372, 589, 995, 1256
AfiI CCNNNNNNNGG 3 cut(s) 294, 545, 1148
AflIII ACRYGT 1 cut(s) 131
AgsI TTSAA 2 cut(s) 443, 829
AjnI CCWGG 3 cut(s) 979, 1066, 1235
AjuI GAANNNNNNNTTGG 2 cut(s) 846, 878
AluBI AGCT 7 cut(s) 383, 393, 491, 563, 632, 728, 1097
AluI AGCT 7 cut(s) 383, 393, 491, 563, 632, 728, 1097
Alw26I GTCTC 1 cut(s) 1256
AlwI GGATC 4 cut(s) 158, 171, 760, 1124
Aor13HI TCCGGA 1 cut(s) 1113
AoxI GGCC 4 cut(s) 502, 514, 909, 977
ApeKI GCWGC 7 cut(s) 244, 593, 667, 758, 806, 1304, 1360
ApoI RAATTY 2 cut(s) 174, 534
Asp700I GAANNNNTTC 2 cut(s) 151, 678
AspLEI GCGC 1 cut(s) 905
AspS9I GGNCC 4 cut(s) 502, 623, 909, 1352
AsuHPI GGTGA 4 cut(s) 106, 328, 1080, 1340
AvaII GGWCC 2 cut(s) 623, 1352
BamHI GGATCC 1 cut(s) 163
BanI GGYRCC 2 cut(s) 1070, 1232
BanII GRGCYC 1 cut(s) 881
BauI CACGAG 1 cut(s) 362
BbrPI CACGTG 2 cut(s) 134, 818
BbsI GAAGAC 1 cut(s) 316
BbvI GCAGC 7 cut(s) 231, 580, 679, 745, 793, 1291, 1347
BccI CCATC 3 cut(s) 13, 234, 274
BcgI CGANNNNNNTGC 2 cut(s) 788, 822
BciT130I CCWGG 3 cut(s) 981, 1068, 1237
BciVI GTATCC 1 cut(s) 1118
BcoDI GTCTC 1 cut(s) 1256
BfaI CTAG 2 cut(s) 299, 701
BfmI CTRYAG 4 cut(s) 179, 564, 1092, 1305
BfuI GTATCC 1 cut(s) 1118
BisI GCNGC 8 cut(s) 245, 594, 668, 759, 807, 962, 1305, 1361
BlsI GCNGC 8 cut(s) 246, 595, 669, 760, 808, 963, 1306, 1362
BmcAI AGTACT 1 cut(s) 589
Bme1390I CCNGG 3 cut(s) 981, 1068, 1237
Bme18I GGWCC 2 cut(s) 623, 1352
BmgT120I GGNCC 4 cut(s) 502, 623, 909, 1352
BmiI GGNNCC 4 cut(s) 165, 1072, 1234, 1407
BmrFI CCNGG 3 cut(s) 981, 1068, 1237
BmsI GCATC 6 cut(s) 34, 56, 315, 776, 1046, 1052
BpiI GAAGAC 1 cut(s) 316
BpmI CTGGAG 1 cut(s) 444
Bsa29I ATCGAT 1 cut(s) 690
BsaAI YACGTR 2 cut(s) 134, 818
BsaJI CCNNGG 4 cut(s) 159, 980, 1074, 1236
BsaWI WCCGGW 3 cut(s) 1113, 1193, 1408
BsaXI ACNNNNNCTCC 2 cut(s) 1308, 1338
Bsc4I CCNNNNNNNGG 3 cut(s) 294, 545, 1148
Bse1I ACTGG 4 cut(s) 141, 458, 500, 547
BseAI TCCGGA 1 cut(s) 1113
BseBI CCWGG 3 cut(s) 981, 1068, 1237
BseCI ATCGAT 1 cut(s) 690
BseDI CCNNGG 4 cut(s) 159, 980, 1074, 1236
BseLI CCNNNNNNNGG 3 cut(s) 294, 545, 1148
BseMII CTCAG 3 cut(s) 246, 501, 598
BseNI ACTGG 4 cut(s) 141, 458, 500, 547
BseXI GCAGC 7 cut(s) 231, 580, 679, 745, 793, 1291, 1347
BseYI CCCAGC 1 cut(s) 499
BsgI GTGCAG 3 cut(s) 744, 918, 1346
Bsh1236I CGCG 2 cut(s) 238, 905
BshFI GGCC 4 cut(s) 504, 516, 911, 979
BshNI GGYRCC 2 cut(s) 1070, 1232
BshVI ATCGAT 1 cut(s) 690
BsiSI CCGG 5 cut(s) 1114, 1194, 1227, 1350, 1409
BslFI GGGAC 1 cut(s) 421
BslI CCNNNNNNNGG 3 cut(s) 294, 545, 1148
BsmAI GTCTC 1 cut(s) 1256
BsmFI GGGAC 1 cut(s) 421
BsmI GAATGC 1 cut(s) 585
BsnI GGCC 4 cut(s) 504, 516, 911, 979
Bsp1286I GDGCHC 1 cut(s) 881
Bsp13I TCCGGA 1 cut(s) 1113
Bsp143I GATC 6 cut(s) 88, 163, 687, 691, 752, 1116
BspACI CCGC 2 cut(s) 867, 961
BspANI GGCC 4 cut(s) 504, 516, 911, 979
BspCNI CTCAG 3 cut(s) 247, 500, 597
BspDI ATCGAT 1 cut(s) 690
BspEI TCCGGA 1 cut(s) 1113
BspFNI CGCG 2 cut(s) 238, 905
BspLI GGNNCC 4 cut(s) 165, 1072, 1234, 1407
BspMAI CTGCAG 1 cut(s) 1309
BspPI GGATC 4 cut(s) 158, 171, 760, 1124
BspQI GCTCTTC 1 cut(s) 459
BspT107I GGYRCC 2 cut(s) 1070, 1232
BsrI ACTGG 4 cut(s) 141, 458, 500, 547
BssECI CCNNGG 4 cut(s) 159, 980, 1074, 1236
BssMI GATC 6 cut(s) 88, 163, 687, 691, 752, 1116
BssSI CACGAG 1 cut(s) 362
BssT1I CCWWGG 1 cut(s) 1074
Bst2BI CACGAG 1 cut(s) 362
Bst2UI CCWGG 3 cut(s) 981, 1068, 1237
Bst4CI ACNGT 2 cut(s) 1189, 1324
Bst6I CTCTTC 1 cut(s) 459
BstAPI GCANNNNNTGC 1 cut(s) 269
BstBAI YACGTR 2 cut(s) 134, 818
BstC8I GCNNGC 3 cut(s) 261, 391, 1383
BstDEI CTNAG 3 cut(s) 255, 487, 584
BstEII GGTNACC 1 cut(s) 1328
BstFNI CGCG 2 cut(s) 238, 905
BstHHI GCGC 1 cut(s) 905
BstKTI GATC 6 cut(s) 91, 166, 690, 694, 755, 1119
BstMAI GTCTC 1 cut(s) 1256
BstMBI GATC 6 cut(s) 88, 163, 687, 691, 752, 1116
BstMWI GCNNNNNNNGC 8 cut(s) 244, 269, 638, 764, 812, 917, 1034, 1232
BstNI CCWGG 3 cut(s) 981, 1068, 1237
BstPI GGTNACC 1 cut(s) 1328
BstSCI CCNGG 3 cut(s) 979, 1066, 1235
BstSFI CTRYAG 4 cut(s) 179, 564, 1092, 1305
BstUI CGCG 2 cut(s) 238, 905
BstV1I GCAGC 7 cut(s) 231, 580, 679, 745, 793, 1291, 1347
BstV2I GAAGAC 1 cut(s) 316
BstX2I RGATCY 3 cut(s) 163, 752, 1116
BstXI CCANNNNNNTGG 2 cut(s) 273, 512
BstYI RGATCY 3 cut(s) 163, 752, 1116
Bsu15I ATCGAT 1 cut(s) 690
BsuI GTATCC 1 cut(s) 1118
BsuRI GGCC 4 cut(s) 504, 516, 911, 979
BsuTUI ATCGAT 1 cut(s) 690
BtgZI GCGATG 1 cut(s) 253
BtsIMutI CAGTG 1 cut(s) 148
Cac8I GCNNGC 3 cut(s) 261, 391, 1383
CfoI GCGC 1 cut(s) 905
Cfr13I GGNCC 4 cut(s) 502, 623, 909, 1352
ClaI ATCGAT 1 cut(s) 690
CseI GACGC 2 cut(s) 219, 713
CsiI ACCWGGT 1 cut(s) 1066
Csp6I GTAC 5 cut(s) 129, 371, 588, 994, 1255
CviAII CATG 4 cut(s) 404, 530, 695, 1171
CviQI GTAC 5 cut(s) 129, 371, 588, 994, 1255
DdeI CTNAG 3 cut(s) 255, 487, 584
DpnI GATC 6 cut(s) 90, 165, 689, 693, 754, 1118
DpnII GATC 6 cut(s) 88, 163, 687, 691, 752, 1116
DraI TTTAAA 1 cut(s) 1339
Eam1104I CTCTTC 1 cut(s) 459
EarI CTCTTC 1 cut(s) 459
EciI GGCGGA 1 cut(s) 882
Eco130I CCWWGG 1 cut(s) 1074
Eco147I AGGCCT 1 cut(s) 979
Eco24I GRGCYC 1 cut(s) 881
Eco47I GGWCC 2 cut(s) 623, 1352
Eco72I CACGTG 2 cut(s) 134, 818
Eco91I GGTNACC 1 cut(s) 1328
EcoO65I GGTNACC 1 cut(s) 1328
EcoRI GAATTC 1 cut(s) 534
EcoRII CCWGG 3 cut(s) 979, 1066, 1235
EcoT14I CCWWGG 1 cut(s) 1074
EcoT22I ATGCAT 1 cut(s) 49
EcoT38I GRGCYC 1 cut(s) 881
ErhI CCWWGG 1 cut(s) 1074
FaeI CATG 4 cut(s) 407, 533, 698, 1174
FaqI GGGAC 1 cut(s) 421
FatI CATG 4 cut(s) 403, 529, 694, 1170
FauNDI CATATG 2 cut(s) 319, 598
FblI GTMKAC 1 cut(s) 233
Fnu4HI GCNGC 8 cut(s) 245, 594, 668, 759, 807, 962, 1305, 1361
FriOI GRGCYC 1 cut(s) 881
Fsp4HI GCNGC 8 cut(s) 245, 594, 668, 759, 807, 962, 1305, 1361
FspBI CTAG 2 cut(s) 299, 701
GlaI GCGC 1 cut(s) 904
GluI GCNGC 8 cut(s) 245, 594, 668, 759, 807, 962, 1305, 1361
GsaI CCCAGC 1 cut(s) 503
GsuI CTGGAG 1 cut(s) 444
HaeIII GGCC 4 cut(s) 504, 516, 911, 979
HapII CCGG 5 cut(s) 1114, 1194, 1227, 1350, 1409
HgaI GACGC 2 cut(s) 219, 713
HhaI GCGC 1 cut(s) 905
Hin1II CATG 4 cut(s) 407, 533, 698, 1174
Hin6I GCGC 1 cut(s) 903
HinP1I GCGC 1 cut(s) 903
HinfI GANTC 5 cut(s) 4, 137, 426, 443, 661
HpaII CCGG 5 cut(s) 1114, 1194, 1227, 1350, 1409
HphI GGTGA 4 cut(s) 106, 328, 1080, 1340
Hpy166II GTNNAC 4 cut(s) 79, 131, 234, 821
Hpy188I TCNGA 3 cut(s) 160, 219, 1421
Hpy188III TCNNGA 5 cut(s) 440, 683, 701, 851, 1114
Hpy8I GTNNAC 4 cut(s) 79, 131, 234, 821
HpyAV CCTTC 1 cut(s) 1365
HpyCH4III ACNGT 2 cut(s) 1189, 1324
HpyCH4IV ACGT 4 cut(s) 28, 133, 817, 1365
HpyF10VI GCNNNNNNNGC 8 cut(s) 244, 269, 638, 764, 812, 917, 1034, 1232
HpyF3I CTNAG 3 cut(s) 255, 487, 584
HpySE526I ACGT 4 cut(s) 28, 133, 817, 1365
Hsp92II CATG 4 cut(s) 407, 533, 698, 1174
HspAI GCGC 1 cut(s) 903
Kpn2I TCCGGA 1 cut(s) 1113
Kzo9I GATC 6 cut(s) 88, 163, 687, 691, 752, 1116
LguI GCTCTTC 1 cut(s) 459
LmnI GCTCC 2 cut(s) 398, 1411
Lsp1109I GCAGC 7 cut(s) 231, 580, 679, 745, 793, 1291, 1347
LweI GCATC 6 cut(s) 34, 56, 315, 776, 1046, 1052
MabI ACCWGGT 1 cut(s) 1066
MaeI CTAG 2 cut(s) 299, 701
MaeII ACGT 4 cut(s) 28, 133, 817, 1365
MaeIII GTNAC 3 cut(s) 196, 1309, 1328
MalI GATC 6 cut(s) 90, 165, 689, 693, 754, 1118
MboI GATC 6 cut(s) 88, 163, 687, 691, 752, 1116
MboII GAAGA 5 cut(s) 53, 147, 316, 446, 767
MflI RGATCY 3 cut(s) 163, 752, 1116
MhlI GDGCHC 1 cut(s) 881
MluCI AATT 6 cut(s) 120, 174, 417, 534, 648, 965
MlyI GAGTC 2 cut(s) 435, 670
MmeI TCCRAC 2 cut(s) 88, 563
Mph1103I ATGCAT 1 cut(s) 49
MroI TCCGGA 1 cut(s) 1113
MroXI GAANNNNTTC 2 cut(s) 151, 678
MseI TTAA 4 cut(s) 279, 948, 1049, 1338
MslI CAYNNNNRTG 3 cut(s) 271, 1175, 1376
MspA1I CMGCKG 1 cut(s) 563
MspI CCGG 5 cut(s) 1114, 1194, 1227, 1350, 1409
MspR9I CCNGG 3 cut(s) 981, 1068, 1237
Mva1269I GAATGC 1 cut(s) 585
MvaI CCWGG 3 cut(s) 981, 1068, 1237
MvnI CGCG 2 cut(s) 238, 905
MwoI GCNNNNNNNGC 8 cut(s) 244, 269, 638, 764, 812, 917, 1034, 1232
NdeI CATATG 2 cut(s) 319, 598
NdeII GATC 6 cut(s) 88, 163, 687, 691, 752, 1116
NlaIII CATG 4 cut(s) 407, 533, 698, 1174
NlaIV GGNNCC 4 cut(s) 165, 1072, 1234, 1407
NmuCI GTSAC 1 cut(s) 1328
NsiI ATGCAT 1 cut(s) 49
PceI AGGCCT 1 cut(s) 979
PciSI GCTCTTC 1 cut(s) 459
PctI GAATGC 1 cut(s) 585
PdmI GAANNNNTTC 2 cut(s) 151, 678
PfeI GAWTC 3 cut(s) 4, 137, 443
PflMI CCANNNNNTGG 1 cut(s) 545
PkrI GCNGC 8 cut(s) 246, 595, 669, 760, 808, 963, 1306, 1362
PleI GAGTC 2 cut(s) 434, 669
PmaCI CACGTG 2 cut(s) 134, 818
PmlI CACGTG 2 cut(s) 134, 818
PpsI GAGTC 2 cut(s) 434, 669
Ppu21I YACGTR 2 cut(s) 134, 818
Psp1406I AACGTT 1 cut(s) 28
Psp6I CCWGG 3 cut(s) 979, 1066, 1235
PspCI CACGTG 2 cut(s) 134, 818
PspEI GGTNACC 1 cut(s) 1328
PspFI CCCAGC 1 cut(s) 499
PspGI CCWGG 3 cut(s) 979, 1066, 1235
PspN4I GGNNCC 4 cut(s) 165, 1072, 1234, 1407
PspPI GGNCC 4 cut(s) 502, 623, 909, 1352
PstI CTGCAG 1 cut(s) 1309
PsuI RGATCY 3 cut(s) 163, 752, 1116
PvuII CAGCTG 1 cut(s) 563
RsaI GTAC 5 cut(s) 130, 372, 589, 995, 1256
RsaNI GTAC 5 cut(s) 129, 371, 588, 994, 1255
RseI CAYNNNNRTG 3 cut(s) 271, 1175, 1376
SapI GCTCTTC 1 cut(s) 459
SaqAI TTAA 4 cut(s) 279, 948, 1049, 1338
SatI GCNGC 8 cut(s) 245, 594, 668, 759, 807, 962, 1305, 1361
Sau3AI GATC 6 cut(s) 88, 163, 687, 691, 752, 1116
Sau96I GGNCC 4 cut(s) 502, 623, 909, 1352
ScaI AGTACT 1 cut(s) 589
SchI GAGTC 2 cut(s) 435, 670
ScrFI CCNGG 3 cut(s) 981, 1068, 1237
SduI GDGCHC 1 cut(s) 881
SexAI ACCWGGT 1 cut(s) 1066
SfaNI GCATC 6 cut(s) 34, 56, 315, 776, 1046, 1052
SfcI CTRYAG 4 cut(s) 179, 564, 1092, 1305
SinI GGWCC 2 cut(s) 623, 1352
SmiMI CAYNNNNRTG 3 cut(s) 271, 1175, 1376
Sse9I AATT 6 cut(s) 120, 174, 417, 534, 648, 965
SseBI AGGCCT 1 cut(s) 979
SsiI CCGC 2 cut(s) 867, 961
SspI AATATT 1 cut(s) 114
SspMI CTAG 2 cut(s) 299, 701
StuI AGGCCT 1 cut(s) 979
StyD4I CCNGG 3 cut(s) 979, 1066, 1235
StyI CCWWGG 1 cut(s) 1074
TaaI ACNGT 2 cut(s) 1189, 1324
TaiI ACGT 4 cut(s) 31, 136, 820, 1368
TaqI TCGA 2 cut(s) 39, 690
TasI AATT 6 cut(s) 120, 174, 417, 534, 648, 965
TatI WGTACW 3 cut(s) 587, 993, 1254
TauI GCSGC 1 cut(s) 964
TfiI GAWTC 3 cut(s) 4, 137, 443
Tru1I TTAA 4 cut(s) 279, 948, 1049, 1338
Tru9I TTAA 4 cut(s) 279, 948, 1049, 1338
TscAI CASTG 1 cut(s) 148
TseFI GTSAC 1 cut(s) 1328
TseI GCWGC 7 cut(s) 244, 593, 667, 758, 806, 1304, 1360
Tsp45I GTSAC 1 cut(s) 1328
TspDTI ATGAA 3 cut(s) 46, 90, 185
TspGWI ACGGA 1 cut(s) 365
TspRI CASTG 1 cut(s) 148
Van91I CCANNNNNTGG 1 cut(s) 545
VpaK11BI GGWCC 2 cut(s) 623, 1352
XapI RAATTY 2 cut(s) 174, 534
XbaI TCTAGA 1 cut(s) 700
XmiI GTMKAC 1 cut(s) 233
XmnI GAANNNNTTC 2 cut(s) 151, 678
XspI CTAG 2 cut(s) 299, 701
ZrmI AGTACT 1 cut(s) 589
Zsp2I ATGCAT 1 cut(s) 49
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.