pycom17g18800
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA M5U methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0007866
Physical Location & Seq
Forward (+)
16250496 .. 16256022
5527 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1671 bp
ATGACACAGACGGTACCAGAAGCAAGACTATCCCCAGCAAAAATGGTGAACGTGCCGAGTCACGGACTGAGGCACGTGAGCGCCATTCGCCCGTGGCTCCCCACCTTCCGTCGCTCCACCTCCCTGGCTTCTCTCTCCTCCCCTTACACCGAAGACTCGGAGCATGCCCAGCCCAAAAGCGGCGACAAGCCCAACTCGTACTTTCCGAAGCGAGGGCAGACTCTGGAGCTGGTGTGCGAGTCTCTGGCTTTCAAAGGGAAAGGTCTATGCAAGGTGGCAGAGACTGGGTTCGTCGTCATGTGCGACCGCGCGCTGCCCGGCGAGCGGTTCGTAGGGAGGGTTACTCGCCGGAAAGGGACCTATGCTGAGGTGACAAAGGTTGAGACAATTTCTCCTCCTTGGGACTTTGTGGACGCTCCTTGTGAGTATGCTTCTTACTGTGGAGGATGTAAAACACAAAATTTGATTTATGAGGCGCAAGTCAAAGCTAAGGAGCAGCAAGTTCACGAGTTGATTATAAACTTTGGAAAGTTTTCTCACACAGAACTGGAATCTTTAAGCATCATGAAGCCCATCGTTCCCTGCGAAATCCAGTTTCATTACAGGAATAAGATGGAGTTCTCATTTGGTTCTCAAAAATGGTTACCCAAGGAAAAATTGATGGAGAGACAAGATGGAATTGAGAACTATGCCCTGGGATTGCATGCTCCTGGATTCTTCGATAAGGTTCTGAATGTTGACAAGTGCTTATTGCAAAGTGAGCCGGCGAATATGGTTCTTGCTGTAGTCCAAGACTGTTGGACAGATCCACAATTAGGTCTCTCTCCTTATGACGTTTACTCTCATGTTGGATTTCTTAAGCATCTAATGCTAAGAACTGGAAGGAATGCGGAGACTGGTCTTCCTGAACTTATGGTTAATTTTGTGACCTCATCTAACAAGCCAGAGCTGCTTAAGCCTCTTCTTGAAAAGATATCAGCTATCCCTGAAGTGGTAAGTGTCATGAACAATATAAATAGTTCTGTTGGTAACACATCTCTTGGGGAGGTGGAATACAATTTGTATGGAAAATCCAGCATCACGGAGAACTTAAGAGGGCTAACATTTCAAATATCAGCAAACTCATTCTTCCAAACAAATACTCACCAGGCAGAGGTTTTATATGAACTCATAGAAGATTGTGCTGGTCTCAGAGGAGATGGCTCAGAGATTGTTCTTGACCTGTTTTGTGGAACCGGCACCATTGGGTTGACACTTGCCAGAAGGGCCAAACATGTTTATGGATATGAAATAATTCCTCAAGCCATTGCAGATGCTTGTCTAAATGCCGAGCTGAATGGCATCCAGAATGCCACATTTGTCCAAGGGGATCTTAACAAAATTGATGAAAGTTTTGGGAAAAATTTTCCTAAGCCCGACATAGTTATTTCAGATCCAAACCGTCCAGGAATGCACATGAAGTTGATAAGATATCTTCTAAAGCTTAAGGCGCCACGGATAGTTTATGTATCGTGTAATCCTGCCACATGTGCTCGTGACCTTGATTATCTCTGTCATGGTGTGACTGAGAAAGGTATAGAAGGCTGTTATAAGCTAAAGAGCGTACAGCCAGTAGACATGTTCCCTCACACTCCTCATATTGAGTGTGTTTGCCTTTTGGAGCTTCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
tRNA_U5-meth_tr

Protein Analysis

557

Amino Acids

62.0

Weight (kDa)

6.58

Isoelectric Point (pI)

41.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA_U5-meth_tr PF05958 364 - 554 8.4e-16 tRNA (Uracil-5-)-methyltransferase
Methyltransf_31 PF13847 403 - 507 4.1e-13 Methyltransferase domain
Methyltransf_15 PF09445 403 - 480 5.3e-06 RNA cap guanine-N2 methyltransferase
Methyltransf_25 PF13649 405 - 463 2.4e-07 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 518, 1590
Acc65I GGTACC 1 cut(s) 13
AccB1I GGYRCC 3 cut(s) 13, 1238, 1489
AccBSI CCGCTC 1 cut(s) 325
AccI GTMKAC 1 cut(s) 1614
AccII CGCG 2 cut(s) 309, 311
AciI CCGC 4 cut(s) 180, 307, 325, 890
AclWI GGATC 3 cut(s) 800, 1377, 1427
AcsI RAATTY 2 cut(s) 460, 1402
AcuI CTGAAG 1 cut(s) 1008
AcvI CACGTG 1 cut(s) 76
AcyI GRCGYC 1 cut(s) 1490
AfaI GTAC 3 cut(s) 15, 200, 1605
AfiI CCNNNNNNNGG 7 cut(s) 62, 179, 212, 324, 815, 991, 1153
AflII CTTAAG 4 cut(s) 857, 953, 1090, 1484
AflIII ACRYGT 3 cut(s) 1273, 1526, 1617
AgsI TTSAA 3 cut(s) 253, 968, 1109
AjnI CCWGG 5 cut(s) 123, 693, 709, 1146, 1444
AluBI AGCT 8 cut(s) 229, 488, 949, 980, 1333, 1483, 1594, 1663
AluI AGCT 8 cut(s) 229, 488, 949, 980, 1333, 1483, 1594, 1663
Alw21I GWGCWC 1 cut(s) 1534
Alw26I GTCTC 7 cut(s) 246, 275, 377, 661, 824, 887, 1193
AlwI GGATC 3 cut(s) 800, 1377, 1427
AlwNI CAGNNNCTG 2 cut(s) 223, 284
AoxI GGCC 1 cut(s) 1266
ApeKI GCWGC 3 cut(s) 313, 496, 949
ApoI RAATTY 2 cut(s) 460, 1402
Asp700I GAANNNNTTC 2 cut(s) 532, 1293
Asp718I GGTACC 1 cut(s) 13
AspLEI GCGC 5 cut(s) 83, 311, 313, 478, 1492
AspS9I GGNCC 2 cut(s) 357, 1266
AsuC2I CCSGG 1 cut(s) 318
AsuHPI GGTGA 3 cut(s) 58, 382, 1136
AvaII GGWCC 1 cut(s) 357
BanI GGYRCC 3 cut(s) 13, 1238, 1489
BauI CACGAG 2 cut(s) 506, 1533
BbrPI CACGTG 1 cut(s) 76
BbsI GAAGAC 2 cut(s) 159, 893
Bbv12I GWGCWC 1 cut(s) 1534
BbvCI CCTCAGC 1 cut(s) 366
BbvI GCAGC 3 cut(s) 300, 508, 936
BccI CCATC 5 cut(s) 581, 607, 655, 668, 1193
BciT130I CCWGG 5 cut(s) 125, 695, 711, 1148, 1446
BcnI CCSGG 1 cut(s) 318
BcoDI GTCTC 7 cut(s) 246, 275, 377, 661, 824, 887, 1193
BfmI CTRYAG 1 cut(s) 783
BfoI RGCGCY 2 cut(s) 84, 1493
BfrI CTTAAG 4 cut(s) 857, 953, 1090, 1484
BglI GCCNNNNNGGC 1 cut(s) 1265
BisI GCNGC 4 cut(s) 181, 314, 497, 950
BlsI GCNGC 4 cut(s) 182, 315, 498, 951
Bme1390I CCNGG 6 cut(s) 125, 318, 695, 711, 1148, 1446
Bme18I GGWCC 1 cut(s) 357
BmgT120I GGNCC 2 cut(s) 357, 1266
BmiI GGNNCC 6 cut(s) 15, 98, 358, 1234, 1240, 1491
BmrFI CCNGG 6 cut(s) 125, 318, 695, 711, 1148, 1446
BmrI ACTGGG 1 cut(s) 294
BmsI GCATC 5 cut(s) 570, 871, 1086, 1303, 1350
BmuI ACTGGG 1 cut(s) 294
BpiI GAAGAC 2 cut(s) 159, 893
BplI GAGNNNNNCTC 2 cut(s) 328, 360
BpmI CTGGAG 1 cut(s) 245
Bpu10I CCTNAGC 3 cut(s) 366, 489, 1410
BpuEI CTTGAG 1 cut(s) 1284
BpuMI CCSGG 1 cut(s) 318
BsaAI YACGTR 1 cut(s) 76
BsaHI GRCGYC 1 cut(s) 1490
BsaI GGTCTC 2 cut(s) 824, 1193
BsaJI CCNNGG 8 cut(s) 92, 123, 398, 648, 693, 694, 1363, 1493
BsaXI ACNNNNNCTCC 4 cut(s) 218, 248, 376, 406
Bsc4I CCNNNNNNNGG 7 cut(s) 62, 179, 212, 324, 815, 991, 1153
Bse118I RCCGGY 2 cut(s) 763, 1235
Bse1I ACTGG 6 cut(s) 289, 552, 592, 883, 901, 1610
Bse3DI GCAATG 1 cut(s) 1305
BseBI CCWGG 5 cut(s) 125, 695, 711, 1148, 1446
BseDI CCNNGG 8 cut(s) 92, 123, 398, 648, 693, 694, 1363, 1493
BseGI GGATG 2 cut(s) 452, 1341
BseLI CCNNNNNNNGG 7 cut(s) 62, 179, 212, 324, 815, 991, 1153
BseMI GCAATG 1 cut(s) 1305
BseMII CTCAG 5 cut(s) 59, 357, 1204, 1218, 1557
BseNI ACTGG 6 cut(s) 289, 552, 592, 883, 901, 1610
BsePI GCGCGC 1 cut(s) 309
BseRI GAGGAG 4 cut(s) 127, 384, 1209, 1623
BseXI GCAGC 3 cut(s) 300, 508, 936
BseYI CCCAGC 2 cut(s) 34, 168
Bsh1236I CGCG 2 cut(s) 309, 311
Bsh1285I CGRYCG 1 cut(s) 307
BshFI GGCC 1 cut(s) 1268
BshNI GGYRCC 3 cut(s) 13, 1238, 1489
BsiEI CGRYCG 1 cut(s) 307
BsiHKAI GWGCWC 1 cut(s) 1534
BsiSI CCGG 4 cut(s) 318, 349, 764, 1236
BslFI GGGAC 2 cut(s) 370, 416
BslI CCNNNNNNNGG 7 cut(s) 62, 179, 212, 324, 815, 991, 1153
BsmAI GTCTC 7 cut(s) 246, 275, 377, 661, 824, 887, 1193
BsmFI GGGAC 2 cut(s) 370, 416
BsmI GAATGC 3 cut(s) 892, 1354, 1455
BsnI GGCC 1 cut(s) 1268
Bso31I GGTCTC 2 cut(s) 824, 1193
Bsp1286I GDGCHC 1 cut(s) 1534
Bsp143I GATC 3 cut(s) 805, 1369, 1432
BspACI CCGC 4 cut(s) 180, 307, 325, 890
BspANI GGCC 1 cut(s) 1268
BspCNI CTCAG 5 cut(s) 60, 358, 1203, 1217, 1558
BspFNI CGCG 2 cut(s) 309, 311
BspHI TCATGA 2 cut(s) 564, 1002
BspLI GGNNCC 6 cut(s) 15, 98, 358, 1234, 1240, 1491
BspPI GGATC 3 cut(s) 800, 1377, 1427
BspT107I GGYRCC 3 cut(s) 13, 1238, 1489
BspTI CTTAAG 4 cut(s) 857, 953, 1090, 1484
BspTNI GGTCTC 2 cut(s) 824, 1193
BsrBI CCGCTC 1 cut(s) 325
BsrDI GCAATG 1 cut(s) 1305
BsrFI RCCGGY 2 cut(s) 763, 1235
BsrI ACTGG 6 cut(s) 289, 552, 592, 883, 901, 1610
BssAI RCCGGY 2 cut(s) 763, 1235
BssECI CCNNGG 8 cut(s) 92, 123, 398, 648, 693, 694, 1363, 1493
BssHII GCGCGC 1 cut(s) 309
BssMI GATC 3 cut(s) 805, 1369, 1432
BssNI GRCGYC 1 cut(s) 1490
BssSI CACGAG 2 cut(s) 506, 1533
BssT1I CCWWGG 3 cut(s) 398, 648, 1363
Bst2BI CACGAG 2 cut(s) 506, 1533
Bst2UI CCWGG 5 cut(s) 125, 695, 711, 1148, 1446
Bst4CI ACNGT 4 cut(s) 13, 440, 797, 1442
Bst6I CTCTTC 1 cut(s) 966
BstACI GRCGYC 1 cut(s) 1490
BstAFI CTTAAG 4 cut(s) 857, 953, 1090, 1484
BstAPI GCANNNNNTGC 1 cut(s) 868
BstBAI YACGTR 1 cut(s) 76
BstC8I GCNNGC 5 cut(s) 165, 311, 323, 705, 765
BstDEI CTNAG 8 cut(s) 68, 366, 489, 872, 1190, 1204, 1410, 1566
BstDSI CCRYGG 2 cut(s) 92, 1493
BstEII GGTNACC 1 cut(s) 642
BstF5I GGATG 2 cut(s) 452, 1341
BstFNI CGCG 2 cut(s) 309, 311
BstH2I RGCGCY 2 cut(s) 84, 1493
BstHHI GCGC 5 cut(s) 83, 311, 313, 478, 1492
BstKTI GATC 3 cut(s) 808, 1372, 1435
BstMAI GTCTC 7 cut(s) 246, 275, 377, 661, 824, 887, 1193
BstMBI GATC 3 cut(s) 805, 1369, 1432
BstMCI CGRYCG 1 cut(s) 307
BstNI CCWGG 5 cut(s) 125, 695, 711, 1148, 1446
BstNSI RCATGY 5 cut(s) 167, 707, 1277, 1530, 1621
BstPI GGTNACC 1 cut(s) 642
BstSCI CCNGG 6 cut(s) 123, 316, 693, 709, 1146, 1444
BstSFI CTRYAG 1 cut(s) 783
BstUI CGCG 2 cut(s) 309, 311
BstV1I GCAGC 3 cut(s) 300, 508, 936
BstV2I GAAGAC 2 cut(s) 159, 893
BstX2I RGATCY 3 cut(s) 805, 1369, 1432
BstXI CCANNNNNNTGG 1 cut(s) 124
BstYI RGATCY 3 cut(s) 805, 1369, 1432
BsuRI GGCC 1 cut(s) 1268
BtgI CCRYGG 2 cut(s) 92, 1493
BtsCI GGATG 2 cut(s) 452, 1341
Cac8I GCNNGC 5 cut(s) 165, 311, 323, 705, 765
CaiI CAGNNNCTG 2 cut(s) 223, 284
CciI TCATGA 2 cut(s) 564, 1002
CfoI GCGC 5 cut(s) 83, 311, 313, 478, 1492
Cfr10I RCCGGY 2 cut(s) 763, 1235
Cfr13I GGNCC 2 cut(s) 357, 1266
CseI GACGC 1 cut(s) 422
Csp6I GTAC 3 cut(s) 14, 199, 1604
CviQI GTAC 3 cut(s) 14, 199, 1604
DdeI CTNAG 8 cut(s) 68, 366, 489, 872, 1190, 1204, 1410, 1566
DinI GGCGCC 1 cut(s) 1491
DpnI GATC 3 cut(s) 807, 1371, 1434
DpnII GATC 3 cut(s) 805, 1369, 1432
Eam1104I CTCTTC 1 cut(s) 966
EarI CTCTTC 1 cut(s) 966
Eco130I CCWWGG 3 cut(s) 398, 648, 1363
Eco31I GGTCTC 2 cut(s) 824, 1193
Eco32I GATATC 2 cut(s) 975, 1472
Eco47I GGWCC 1 cut(s) 357
Eco57I CTGAAG 1 cut(s) 1008
Eco72I CACGTG 1 cut(s) 76
Eco91I GGTNACC 1 cut(s) 642
EcoO109I RGGNCCY 1 cut(s) 357
EcoO65I GGTNACC 1 cut(s) 642
EcoRII CCWGG 5 cut(s) 123, 693, 709, 1146, 1444
EcoRV GATATC 2 cut(s) 975, 1472
EcoT14I CCWWGG 3 cut(s) 398, 648, 1363
EgeI GGCGCC 1 cut(s) 1491
EheI GGCGCC 1 cut(s) 1491
ErhI CCWWGG 3 cut(s) 398, 648, 1363
FalI AAGNNNNNCTT 2 cut(s) 1356, 1388
FaqI GGGAC 2 cut(s) 370, 416
FblI GTMKAC 1 cut(s) 1614
Fnu4HI GCNGC 4 cut(s) 181, 314, 497, 950
FokI GGATG 2 cut(s) 459, 1328
Fsp4HI GCNGC 4 cut(s) 181, 314, 497, 950
GlaI GCGC 5 cut(s) 82, 310, 312, 477, 1491
GluI GCNGC 4 cut(s) 181, 314, 497, 950
GsaI CCCAGC 2 cut(s) 38, 172
GsuI CTGGAG 1 cut(s) 245
HaeII RGCGCY 2 cut(s) 84, 1493
HaeIII GGCC 1 cut(s) 1268
HapII CCGG 4 cut(s) 318, 349, 764, 1236
HgaI GACGC 1 cut(s) 422
HhaI GCGC 5 cut(s) 83, 311, 313, 478, 1492
Hin1I GRCGYC 1 cut(s) 1490
Hin6I GCGC 5 cut(s) 81, 309, 311, 476, 1490
HinP1I GCGC 5 cut(s) 81, 309, 311, 476, 1490
HincII GTYRAC 2 cut(s) 739, 1251
HindII GTYRAC 2 cut(s) 739, 1251
HindIII AAGCTT 1 cut(s) 1481
HinfI GANTC 6 cut(s) 58, 155, 220, 239, 551, 714
HpaII CCGG 4 cut(s) 318, 349, 764, 1236
HphI GGTGA 3 cut(s) 58, 382, 1136
Hpy166II GTNNAC 7 cut(s) 49, 412, 505, 739, 838, 1251, 1615
Hpy188I TCNGA 6 cut(s) 160, 207, 732, 1193, 1207, 1432
Hpy188III TCNNGA 9 cut(s) 224, 506, 565, 905, 965, 1003, 1217, 1345, 1535
Hpy8I GTNNAC 7 cut(s) 49, 412, 505, 739, 838, 1251, 1615
Hpy99I CGWCG 2 cut(s) 114, 296
HpyAV CCTTC 4 cut(s) 115, 876, 1257, 1574
HpyCH4III ACNGT 4 cut(s) 13, 440, 797, 1442
HpyCH4IV ACGT 3 cut(s) 51, 75, 834
HpyCH4V TGCA 5 cut(s) 270, 703, 754, 1310, 1453
HpyF3I CTNAG 8 cut(s) 68, 366, 489, 872, 1190, 1204, 1410, 1566
HpySE526I ACGT 3 cut(s) 51, 75, 834
Hsp92I GRCGYC 1 cut(s) 1490
HspAI GCGC 5 cut(s) 81, 309, 311, 476, 1490
KasI GGCGCC 1 cut(s) 1489
KpnI GGTACC 1 cut(s) 17
KroI GCCGGC 1 cut(s) 763
KroNI GCCGGC 1 cut(s) 765
Kzo9I GATC 3 cut(s) 805, 1369, 1432
LmnI GCTCC 8 cut(s) 102, 119, 160, 226, 421, 493, 712, 1660
Lsp1109I GCAGC 3 cut(s) 300, 508, 936
LweI GCATC 5 cut(s) 570, 871, 1086, 1303, 1350
MaeII ACGT 3 cut(s) 51, 75, 834
MaeIII GTNAC 8 cut(s) 59, 340, 370, 642, 925, 1028, 1535, 1561
MalI GATC 3 cut(s) 807, 1371, 1434
MbiI CCGCTC 1 cut(s) 325
MboI GATC 3 cut(s) 805, 1369, 1432
MboII GAAGA 7 cut(s) 164, 709, 893, 953, 1120, 1187, 1466
MflI RGATCY 3 cut(s) 805, 1369, 1432
MhlI GDGCHC 1 cut(s) 1534
Mly113I GGCGCC 1 cut(s) 1490
MlyI GAGTC 4 cut(s) 67, 149, 214, 248
MmeI TCCRAC 2 cut(s) 779, 829
MroNI GCCGGC 1 cut(s) 763
MroXI GAANNNNTTC 2 cut(s) 532, 1293
MseI TTAA 7 cut(s) 557, 858, 918, 954, 1091, 1374, 1485
MslI CAYNNNNRTG 1 cut(s) 1278
MspCI CTTAAG 4 cut(s) 857, 953, 1090, 1484
MspI CCGG 4 cut(s) 318, 349, 764, 1236
MspR9I CCNGG 6 cut(s) 125, 318, 695, 711, 1148, 1446
Mva1269I GAATGC 3 cut(s) 892, 1354, 1455
MvaI CCWGG 5 cut(s) 125, 695, 711, 1148, 1446
MvnI CGCG 2 cut(s) 309, 311
NaeI GCCGGC 1 cut(s) 765
NarI GGCGCC 1 cut(s) 1490
NciI CCSGG 1 cut(s) 318
NdeII GATC 3 cut(s) 805, 1369, 1432
NgoMIV GCCGGC 1 cut(s) 763
NlaIV GGNNCC 6 cut(s) 15, 98, 358, 1234, 1240, 1491
NmeAIII GCCGAG 2 cut(s) 81, 1354
NmuCI GTSAC 5 cut(s) 59, 370, 925, 1535, 1561
NspI RCATGY 5 cut(s) 167, 707, 1277, 1530, 1621
PaeI GCATGC 2 cut(s) 167, 707
PagI TCATGA 2 cut(s) 564, 1002
PasI CCCWGGG 1 cut(s) 694
PauI GCGCGC 1 cut(s) 309
PciI ACATGT 3 cut(s) 1273, 1526, 1617
PcsI WCGNNNNNNNCGW 3 cut(s) 203, 300, 582
PctI GAATGC 3 cut(s) 892, 1354, 1455
PdiI GCCGGC 1 cut(s) 765
PdmI GAANNNNTTC 2 cut(s) 532, 1293
PfeI GAWTC 2 cut(s) 551, 714
PfoI TCCNGGA 2 cut(s) 709, 1444
PkrI GCNGC 4 cut(s) 182, 315, 498, 951
PleI GAGTC 4 cut(s) 66, 149, 214, 247
PluTI GGCGCC 1 cut(s) 1493
PmaCI CACGTG 1 cut(s) 76
PmlI CACGTG 1 cut(s) 76
PpsI GAGTC 4 cut(s) 66, 149, 214, 247
Ppu21I YACGTR 1 cut(s) 76
PpuMI RGGWCCY 1 cut(s) 357
PscI ACATGT 3 cut(s) 1273, 1526, 1617
PsiI TTATAA 2 cut(s) 518, 1590
Psp5II RGGWCCY 1 cut(s) 357
Psp6I CCWGG 5 cut(s) 123, 693, 709, 1146, 1444
PspCI CACGTG 1 cut(s) 76
PspEI GGTNACC 1 cut(s) 642
PspFI CCCAGC 2 cut(s) 34, 168
PspGI CCWGG 5 cut(s) 123, 693, 709, 1146, 1444
PspN4I GGNNCC 6 cut(s) 15, 98, 358, 1234, 1240, 1491
PspPI GGNCC 2 cut(s) 357, 1266
PspPPI RGGWCCY 1 cut(s) 357
PstNI CAGNNNCTG 2 cut(s) 223, 284
PsuI RGATCY 3 cut(s) 805, 1369, 1432
PteI GCGCGC 1 cut(s) 309
RsaI GTAC 3 cut(s) 15, 200, 1605
RsaNI GTAC 3 cut(s) 14, 199, 1604
RseI CAYNNNNRTG 1 cut(s) 1278
SaqAI TTAA 7 cut(s) 557, 858, 918, 954, 1091, 1374, 1485
SatI GCNGC 4 cut(s) 181, 314, 497, 950
Sau3AI GATC 3 cut(s) 805, 1369, 1432
Sau96I GGNCC 2 cut(s) 357, 1266
SchI GAGTC 4 cut(s) 67, 149, 214, 248
ScrFI CCNGG 6 cut(s) 125, 318, 695, 711, 1148, 1446
SduI GDGCHC 1 cut(s) 1534
SfaNI GCATC 5 cut(s) 570, 871, 1086, 1303, 1350
SfcI CTRYAG 1 cut(s) 783
SfoI GGCGCC 1 cut(s) 1491
SinI GGWCC 1 cut(s) 357
SmiMI CAYNNNNRTG 1 cut(s) 1278
SmlI CTYRAG 5 cut(s) 857, 953, 1090, 1299, 1484
SmoI CTYRAG 5 cut(s) 857, 953, 1090, 1299, 1484
SphI GCATGC 2 cut(s) 167, 707
SsiI CCGC 4 cut(s) 180, 307, 325, 890
SspDI GGCGCC 1 cut(s) 1489
StyD4I CCNGG 6 cut(s) 123, 316, 693, 709, 1146, 1444
StyI CCWWGG 3 cut(s) 398, 648, 1363
TaaI ACNGT 4 cut(s) 13, 440, 797, 1442
TaiI ACGT 3 cut(s) 54, 78, 837
TaqI TCGA 1 cut(s) 720
TauI GCSGC 1 cut(s) 183
TfiI GAWTC 2 cut(s) 551, 714
Tru1I TTAA 7 cut(s) 557, 858, 918, 954, 1091, 1374, 1485
Tru9I TTAA 7 cut(s) 557, 858, 918, 954, 1091, 1374, 1485
TseFI GTSAC 5 cut(s) 59, 370, 925, 1535, 1561
TseI GCWGC 3 cut(s) 313, 496, 949
Tsp45I GTSAC 5 cut(s) 59, 370, 925, 1535, 1561
TspDTI ATGAA 7 cut(s) 581, 587, 1019, 1179, 1302, 1401, 1472
TspGWI ACGGA 4 cut(s) 78, 98, 1097, 1510
Vha464I CTTAAG 4 cut(s) 857, 953, 1090, 1484
VpaK11BI GGWCC 1 cut(s) 357
XapI RAATTY 2 cut(s) 460, 1402
XceI RCATGY 5 cut(s) 167, 707, 1277, 1530, 1621
XmiI GTMKAC 1 cut(s) 1614
XmnI GAANNNNTTC 2 cut(s) 532, 1293
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.