pycom17g19700

2Fe-2S iron-sulfur cluster binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0013153
Physical Location & Seq
Forward (+)
17628055 .. 17629307
1253 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 600 bp
ATGGTTGCTCTCCAACTGAGCTCTTCTTGTTCATCTTTTTGCTCCCTCCCATTTAACTTGATCAACTCCAACATTACCAACTCCCATTCAACTCTCAGACCTTCCAAGCCCCTCATCAGTTTCTCCAGAGCAAAAATTAGAGCAGTTAGCGCATCCCAAGAGAGCCAATCTGAAGCCATTCAACCTGAGCAACCCCCTTCCGTGGATTTCGCATTTGTTAGTTCAGTTTTACTTCCTGACCAAACACCAGATGTGCATCTTCGTCAAGCCTGTGGAGGGCAGAAACTTAGGAATATAATGTTGGATTCAAATATCGATTTGTATGGACCATACAGTAGAGTTCTGCTGAATTGTGCTGGAGGAGGAACCTGCGGGACATGCATGGTGGAGATTGTTCAAGGAAAAGAGCTGTTAACCCCTCGCACAGACAAAGAGAAGGAACACCTTAAAAAGAAACCAAAAAATTGGAGACTTGCTTGCCAAACCACAGTTGGGAAACCTGACTCTAGAGGGCTGGTTGTTGTCCAACAGCTACCGGAATGGAAAGCCCATGAATGGAAATATGAAGAAGTCCTGGTAGACCCTTCCCAGTCTTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Fer2

Protein Analysis

200

Amino Acids

21.95

Weight (kDa)

8.47

Isoelectric Point (pI)

54.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fer2 PF00111 116 - 164 1.7e-08 2Fe-2S iron-sulfur cluster binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013153)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G16250
fragaria_vesca FvH4_6g31980 FvH4_6g31980
malus_domestica MD09G1207300.v1.1 MD17G1188300.v1.1
prunus_persica Prupe.3G063600_v2.0.a1 Prupe.3G063600_v2.0.a1
pyrus_communis pycom17g19700
rosa_chinensis RchiOBHm_Chr2g0139561
rosa_laevigata RLG00000019819
rosa_multiflora Rmu_sc0003427.1_g000005
rosa_roxburghii Rroxscaffold_2G00105390
rosa_rugosa Rorug02G0358400
rosa_samantha Rh2AG408200 Rh2BG418800 Rh2CG394200 Rh2DG427900
rosa_wichuraiana Rw2G033540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 377
AccI GTMKAC 1 cut(s) 579
AciI CCGC 1 cut(s) 372
AcuI CTGAAG 1 cut(s) 192
AfiI CCNNNNNNNGG 4 cut(s) 202, 276, 492, 555
AgsI TTSAA 4 cut(s) 90, 182, 309, 398
AjnI CCWGG 1 cut(s) 573
AjuI GAANNNNNNNTTGG 2 cut(s) 284, 316
AluBI AGCT 3 cut(s) 21, 409, 532
AluI AGCT 3 cut(s) 21, 409, 532
Alw21I GWGCWC 1 cut(s) 23
Alw26I GTCTC 1 cut(s) 463
Asp700I GAANNNNTTC 1 cut(s) 177
AspLEI GCGC 1 cut(s) 152
AspS9I GGNCC 1 cut(s) 326
AvaII GGWCC 1 cut(s) 326
BanII GRGCYC 1 cut(s) 23
BbsI GAAGAC 1 cut(s) 585
Bbv12I GWGCWC 1 cut(s) 23
BciT130I CCWGG 1 cut(s) 575
BclI TGATCA 1 cut(s) 60
BcoDI GTCTC 1 cut(s) 463
BfaI CTAG 1 cut(s) 507
BfuAI ACCTGC 1 cut(s) 377
Bme1390I CCNGG 1 cut(s) 575
Bme18I GGWCC 1 cut(s) 326
BmgT120I GGNCC 1 cut(s) 326
BmiI GGNNCC 1 cut(s) 367
BmrFI CCNGG 1 cut(s) 575
BmrI ACTGGG 1 cut(s) 583
BmsI GCATC 2 cut(s) 161, 265
BmuI ACTGGG 1 cut(s) 583
BpiI GAAGAC 1 cut(s) 585
BpmI CTGGAG 2 cut(s) 109, 378
Bpu10I CCTNAGC 1 cut(s) 186
Bsa29I ATCGAT 1 cut(s) 315
BsaBI GATNNNNATC 1 cut(s) 255
BsaJI CCNNGG 1 cut(s) 201
BsaWI WCCGGW 1 cut(s) 535
Bsc4I CCNNNNNNNGG 4 cut(s) 202, 276, 492, 555
Bse1I ACTGG 1 cut(s) 589
Bse8I GATNNNNATC 1 cut(s) 255
BseBI CCWGG 1 cut(s) 575
BseCI ATCGAT 1 cut(s) 315
BseDI CCNNGG 1 cut(s) 201
BseGI GGATG 1 cut(s) 152
BseJI GATNNNNATC 1 cut(s) 255
BseLI CCNNNNNNNGG 4 cut(s) 202, 276, 492, 555
BseMII CTCAG 3 cut(s) 8, 109, 177
BseNI ACTGG 1 cut(s) 589
BseRI GAGGAG 1 cut(s) 375
BshVI ATCGAT 1 cut(s) 315
BsiHKAI GWGCWC 1 cut(s) 23
BsiSI CCGG 1 cut(s) 536
BslFI GGGAC 1 cut(s) 388
BslI CCNNNNNNNGG 4 cut(s) 202, 276, 492, 555
BsmAI GTCTC 1 cut(s) 463
BsmFI GGGAC 1 cut(s) 388
Bsp1286I GDGCHC 1 cut(s) 23
Bsp143I GATC 1 cut(s) 60
BspACI CCGC 1 cut(s) 372
BspCNI CTCAG 3 cut(s) 9, 108, 178
BspDI ATCGAT 1 cut(s) 315
BspLI GGNNCC 1 cut(s) 367
BspMI ACCTGC 1 cut(s) 377
BspQI GCTCTTC 1 cut(s) 28
BsrI ACTGG 1 cut(s) 589
BssECI CCNNGG 1 cut(s) 201
BssMI GATC 1 cut(s) 60
Bst2UI CCWGG 1 cut(s) 575
Bst4CI ACNGT 2 cut(s) 335, 490
Bst6I CTCTTC 1 cut(s) 28
BstC8I GCNNGC 1 cut(s) 478
BstDEI CTNAG 4 cut(s) 17, 95, 186, 287
BstDSI CCRYGG 1 cut(s) 201
BstF5I GGATG 1 cut(s) 152
BstHHI GCGC 1 cut(s) 152
BstKTI GATC 1 cut(s) 63
BstMAI GTCTC 1 cut(s) 463
BstMBI GATC 1 cut(s) 60
BstMWI GCNNNNNNNGC 2 cut(s) 149, 378
BstNI CCWGG 1 cut(s) 575
BstNSI RCATGY 1 cut(s) 381
BstSCI CCNGG 1 cut(s) 573
BstV2I GAAGAC 1 cut(s) 585
BstXI CCANNNNNNTGG 1 cut(s) 465
Bsu15I ATCGAT 1 cut(s) 315
BsuTUI ATCGAT 1 cut(s) 315
BtgI CCRYGG 1 cut(s) 201
BtsCI GGATG 1 cut(s) 152
BveI ACCTGC 1 cut(s) 377
Cac8I GCNNGC 1 cut(s) 478
CfoI GCGC 1 cut(s) 152
Cfr13I GGNCC 1 cut(s) 326
ClaI ATCGAT 1 cut(s) 315
CviAII CATG 3 cut(s) 378, 382, 551
CviJI RGCY 9 cut(s) 21, 109, 165, 176, 269, 409, 514, 532, 548
CviKI_1 RGCY 9 cut(s) 21, 109, 165, 176, 269, 409, 514, 532, 548
DdeI CTNAG 4 cut(s) 17, 95, 186, 287
DpnI GATC 1 cut(s) 62
DpnII GATC 1 cut(s) 60
Eam1104I CTCTTC 1 cut(s) 28
EarI CTCTTC 1 cut(s) 28
Ecl136II GAGCTC 1 cut(s) 21
Eco24I GRGCYC 1 cut(s) 23
Eco47I GGWCC 1 cut(s) 326
Eco53kI GAGCTC 1 cut(s) 21
Eco57I CTGAAG 1 cut(s) 192
EcoICRI GAGCTC 1 cut(s) 21
EcoRII CCWGG 1 cut(s) 573
EcoT22I ATGCAT 1 cut(s) 383
EcoT38I GRGCYC 1 cut(s) 23
FaeI CATG 3 cut(s) 381, 385, 554
FaiI YATR 8 cut(s) 296, 324, 331, 379, 383, 552, 564, 598
FaqI GGGAC 1 cut(s) 388
FatI CATG 3 cut(s) 377, 381, 550
FauI CCCGC 1 cut(s) 365
FbaI TGATCA 1 cut(s) 60
FblI GTMKAC 1 cut(s) 579
FokI GGATG 1 cut(s) 139
FriOI GRGCYC 1 cut(s) 23
FspBI CTAG 1 cut(s) 507
GlaI GCGC 1 cut(s) 151
GsuI CTGGAG 2 cut(s) 109, 378
HapII CCGG 1 cut(s) 536
HhaI GCGC 1 cut(s) 152
Hin1II CATG 3 cut(s) 381, 385, 554
Hin6I GCGC 1 cut(s) 150
HinP1I GCGC 1 cut(s) 150
HincII GTYRAC 1 cut(s) 414
HindII GTYRAC 1 cut(s) 414
HinfI GANTC 2 cut(s) 305, 503
HpaI GTTAAC 1 cut(s) 414
HpaII CCGG 1 cut(s) 536
Hpy166II GTNNAC 2 cut(s) 414, 580
Hpy188I TCNGA 2 cut(s) 98, 172
Hpy188III TCNNGA 3 cut(s) 126, 236, 507
Hpy8I GTNNAC 2 cut(s) 414, 580
HpyAV CCTTC 4 cut(s) 111, 207, 430, 594
HpyCH4III ACNGT 2 cut(s) 335, 490
HpyCH4V TGCA 2 cut(s) 256, 381
HpyF10VI GCNNNNNNNGC 2 cut(s) 149, 378
HpyF3I CTNAG 4 cut(s) 17, 95, 186, 287
Hsp92II CATG 3 cut(s) 381, 385, 554
HspAI GCGC 1 cut(s) 150
Ksp22I TGATCA 1 cut(s) 60
KspAI GTTAAC 1 cut(s) 414
Kzo9I GATC 1 cut(s) 60
LguI GCTCTTC 1 cut(s) 28
LmnI GCTCC 1 cut(s) 47
LweI GCATC 2 cut(s) 161, 265
MaeI CTAG 1 cut(s) 507
MalI GATC 1 cut(s) 62
MboI GATC 1 cut(s) 60
MboII GAAGA 4 cut(s) 15, 251, 578, 585
MhlI GDGCHC 1 cut(s) 23
MluCI AATT 3 cut(s) 135, 349, 463
MlyI GAGTC 1 cut(s) 497
MmeI TCCRAC 4 cut(s) 37, 93, 282, 550
MnlI CCTC 7 cut(s) 56, 122, 269, 353, 356, 429, 503
Mph1103I ATGCAT 1 cut(s) 383
MroXI GAANNNNTTC 1 cut(s) 177
MseI TTAA 3 cut(s) 54, 413, 447
MspI CCGG 1 cut(s) 536
MspR9I CCNGG 1 cut(s) 575
MvaI CCWGG 1 cut(s) 575
MwoI GCNNNNNNNGC 2 cut(s) 149, 378
NdeII GATC 1 cut(s) 60
NlaIII CATG 3 cut(s) 381, 385, 554
NlaIV GGNNCC 1 cut(s) 367
NsiI ATGCAT 1 cut(s) 383
NspI RCATGY 1 cut(s) 381
PciSI GCTCTTC 1 cut(s) 28
PdmI GAANNNNTTC 1 cut(s) 177
PfeI GAWTC 1 cut(s) 305
PleI GAGTC 1 cut(s) 497
PpsI GAGTC 1 cut(s) 497
Psp124BI GAGCTC 1 cut(s) 23
Psp6I CCWGG 1 cut(s) 573
PspGI CCWGG 1 cut(s) 573
PspN4I GGNNCC 1 cut(s) 367
PspPI GGNCC 1 cut(s) 326
SacI GAGCTC 1 cut(s) 23
SapI GCTCTTC 1 cut(s) 28
SaqAI TTAA 3 cut(s) 54, 413, 447
Sau3AI GATC 1 cut(s) 60
Sau96I GGNCC 1 cut(s) 326
SchI GAGTC 1 cut(s) 497
ScrFI CCNGG 1 cut(s) 575
SduI GDGCHC 1 cut(s) 23
SetI ASST 8 cut(s) 23, 103, 187, 371, 411, 447, 502, 534
SfaNI GCATC 2 cut(s) 161, 265
SinI GGWCC 1 cut(s) 326
Sse9I AATT 3 cut(s) 135, 349, 463
SsiI CCGC 1 cut(s) 372
SspMI CTAG 1 cut(s) 507
SstI GAGCTC 1 cut(s) 23
StyD4I CCNGG 1 cut(s) 573
TaaI ACNGT 2 cut(s) 335, 490
TaqI TCGA 1 cut(s) 315
TasI AATT 3 cut(s) 135, 349, 463
TfiI GAWTC 1 cut(s) 305
Tru1I TTAA 3 cut(s) 54, 413, 447
Tru9I TTAA 3 cut(s) 54, 413, 447
TspDTI ATGAA 4 cut(s) 21, 567, 579, 585
TspGWI ACGGA 1 cut(s) 190
VpaK11BI GGWCC 1 cut(s) 326
XbaI TCTAGA 1 cut(s) 506
XceI RCATGY 1 cut(s) 381
XcmI CCANNNNNNNNNTGG 1 cut(s) 488
XmiI GTMKAC 1 cut(s) 579
XmnI GAANNNNTTC 1 cut(s) 177
XspI CTAG 1 cut(s) 507
Zsp2I ATGCAT 1 cut(s) 383
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.