pycom17g20690

Protein of unknown function (DUF642)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0005921
Physical Location & Seq
Forward (+)
19117382 .. 19118944
1563 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom17g20690.1

Sequence Viewer

Length: 1020 bp
ATGATGTCAGTTAGCTTATTACTCAACGGCAACTTCGAGTACGGTCCAAAGCATTCAGAACTCAAAGGCACAGTAGTGACGAACCCTAATGCAATTCCCAACTGGGAAATATCAGGCTTTGTCGAGTACATAAAATCAGGTCAAAAACAAGGTGACATGCTGCTGGTCGTCCCTGAGGGCGGCTTCGCAGTGAGGCTCGGGAAGGAGGCCTTGATCAAGCAGAAGTTGAAGCTCACAAAAGGCGGCTTCTACTCCATCACATTCACCGCTGGTCGGACGTGCGGGCAGGAGGAGAAGCTGAACGTGTCCGTCAATCCCAACGTTGAAGAAAATGACTGGGGAATAATACCAATCCAGACAATGTACAGCTGCAATGGATGGGATTCGTATGCATGGGGTTTTAATGCAGATTTTGATGACCCTGCTTGTGGCCCACTTATTGATTCTGTTGCTTTGAAGCTCTTGGAACCTCCTAAACGTACTAGAGCCAACTTGTTGAAAAATGGAAACTTTGAAGAAGGGCCCTATGTGTTGCCCACCGCATCATGGGGAGTCCTAATCCCACCCCGCACAGAAGACGACCACAGCCCACTCCCAGGCTGGATCATCGAGTCTCTCAAGGCCGTTAAGTACATTGACTCCAAGCACTTCTTCGTACCCGAAGGCCGTAGAGCCATCGAGCTTGTCGGCGGCAAAGAAAGCGCATTGGCCCAAGTCGTCTTCACCAAGCCTGGCAAGCTCTACGCCCTTACATTCTCCATAGGTGACTCCAACAACGCCTGCGAAGGCTCCCTCATCGTCGAGGCGTTTGCCGGCAAAGACACCCTCAAGGTGCCGTACCAGTCCAAAGGCAAAGGTGGGTTCAAGCGCGCGAGGCTCCTTTTCACCGCCGTGGGGCCTCGAACCAGGATCATGTTCTACAGCACGTTTTACACCATGTCCGCCGACCACTCTGGGTCTCTGTGCGGCCCCATCATTGACGATGTGAAGTTGCTTAGCGTTCGTAAAGTACGTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF642

Protein Analysis

340

Amino Acids

37.21

Weight (kDa)

8.63

Isoelectric Point (pI)

34.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF642 PF04862 6 - 153 2.8e-52 Protein of unknown function (DUF642)
DUF642 PF04862 164 - 331 9.5e-17 Protein of unknown function (DUF642)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 832
AccII CGCG 2 cut(s) 870, 872
AclI AACGTT 1 cut(s) 321
AclWI GGATC 2 cut(s) 611, 917
AfaI GTAC 8 cut(s) 41, 128, 365, 481, 632, 657, 839, 1011
AfiI CCNNNNNNNGG 6 cut(s) 179, 273, 428, 546, 596, 894
AflIII ACRYGT 2 cut(s) 303, 1012
AgsI TTSAA 6 cut(s) 229, 326, 457, 499, 515, 865
AjiI CACGTC 1 cut(s) 279
AjnI CCWGG 3 cut(s) 595, 730, 905
AjuI GAANNNNNNNTTGG 2 cut(s) 635, 667
AleI CACNNNNGTG 2 cut(s) 74, 890
AluBI AGCT 7 cut(s) 15, 232, 298, 369, 460, 682, 739
AluI AGCT 7 cut(s) 15, 232, 298, 369, 460, 682, 739
Alw26I GTCTC 2 cut(s) 618, 963
AlwI GGATC 2 cut(s) 611, 917
Ama87I CYCGRG 1 cut(s) 197
AoxI GGCC 8 cut(s) 207, 430, 521, 621, 664, 708, 896, 967
ApaI GGGCCC 1 cut(s) 525
ApeKI GCWGC 2 cut(s) 160, 369
AspLEI GCGC 3 cut(s) 704, 870, 872
AspS9I GGNCC 7 cut(s) 44, 431, 521, 522, 709, 896, 968
AsuHPI GGTGA 5 cut(s) 164, 256, 715, 776, 877
AvaI CYCGRG 1 cut(s) 197
AvaII GGWCC 1 cut(s) 44
AxyI CCTNAGG 1 cut(s) 174
BaeGI GKGCMC 1 cut(s) 525
BanI GGYRCC 1 cut(s) 832
BanII GRGCYC 1 cut(s) 525
BbsI GAAGAC 2 cut(s) 582, 712
BbvI GCAGC 2 cut(s) 147, 356
BccI CCATC 4 cut(s) 263, 372, 683, 980
BceAI ACGGC 5 cut(s) 43, 608, 651, 820, 875
BcgI CGANNNNNNTGC 2 cut(s) 791, 825
BciT130I CCWGG 3 cut(s) 597, 732, 907
BclI TGATCA 1 cut(s) 213
BcoDI GTCTC 2 cut(s) 618, 963
BfaI CTAG 1 cut(s) 483
BfmI CTRYAG 1 cut(s) 919
BisI GCNGC 6 cut(s) 161, 181, 244, 370, 691, 967
BlpI GCTNAGC 1 cut(s) 995
BlsI GCNGC 6 cut(s) 162, 182, 245, 371, 692, 968
Bme1390I CCNGG 3 cut(s) 597, 732, 907
Bme18I GGWCC 1 cut(s) 44
BmeT110I CYCGRG 1 cut(s) 197
BmgBI CACGTC 1 cut(s) 279
BmgT120I GGNCC 7 cut(s) 44, 431, 521, 522, 709, 896, 968
BmiI GGNNCC 7 cut(s) 468, 523, 790, 834, 878, 897, 970
BmrFI CCNGG 3 cut(s) 597, 732, 907
BmrI ACTGGG 2 cut(s) 112, 346
BmsI GCATC 1 cut(s) 551
BmuI ACTGGG 2 cut(s) 112, 346
BpiI GAAGAC 2 cut(s) 582, 712
Bpu1102I GCTNAGC 1 cut(s) 995
BpuEI CTTGAG 2 cut(s) 602, 812
BsaAI YACGTR 1 cut(s) 1013
BsaI GGTCTC 1 cut(s) 963
BsaJI CCNNGG 2 cut(s) 595, 891
BsaXI ACNNNNNCTCC 2 cut(s) 623, 653
Bsc4I CCNNNNNNNGG 6 cut(s) 179, 273, 428, 546, 596, 894
Bse118I RCCGGY 1 cut(s) 812
Bse1I ACTGG 3 cut(s) 107, 341, 841
Bse21I CCTNAGG 1 cut(s) 174
Bse3DI GCAATG 1 cut(s) 379
BseBI CCWGG 3 cut(s) 597, 732, 907
BseDI CCNNGG 2 cut(s) 595, 891
BseGI GGATG 1 cut(s) 383
BseLI CCNNNNNNNGG 6 cut(s) 179, 273, 428, 546, 596, 894
BseMI GCAATG 1 cut(s) 379
BseMII CTCAG 1 cut(s) 165
BseNI ACTGG 3 cut(s) 107, 341, 841
BsePI GCGCGC 1 cut(s) 868
BseRI GAGGAG 1 cut(s) 305
BseSI GKGCMC 1 cut(s) 525
BseXI GCAGC 2 cut(s) 147, 356
Bsh1236I CGCG 2 cut(s) 870, 872
BshFI GGCC 8 cut(s) 209, 432, 523, 623, 666, 710, 898, 969
BshNI GGYRCC 1 cut(s) 832
BsiHKCI CYCGRG 1 cut(s) 197
BsiSI CCGG 1 cut(s) 813
BslFI GGGAC 1 cut(s) 155
BslI CCNNNNNNNGG 6 cut(s) 179, 273, 428, 546, 596, 894
BsmAI GTCTC 2 cut(s) 618, 963
BsmFI GGGAC 1 cut(s) 155
BsmI GAATGC 1 cut(s) 52
BsnI GGCC 8 cut(s) 209, 432, 523, 623, 666, 710, 898, 969
Bso31I GGTCTC 1 cut(s) 963
BsoBI CYCGRG 1 cut(s) 197
Bsp120I GGGCCC 1 cut(s) 521
Bsp1286I GDGCHC 1 cut(s) 525
Bsp1407I TGTACA 1 cut(s) 363
Bsp143I GATC 3 cut(s) 213, 603, 909
Bsp1720I GCTNAGC 1 cut(s) 995
BspANI GGCC 8 cut(s) 209, 432, 523, 623, 666, 710, 898, 969
BspCNI CTCAG 1 cut(s) 166
BspFNI CGCG 2 cut(s) 870, 872
BspLI GGNNCC 7 cut(s) 468, 523, 790, 834, 878, 897, 970
BspPI GGATC 2 cut(s) 611, 917
BspT107I GGYRCC 1 cut(s) 832
BspTNI GGTCTC 1 cut(s) 963
BsrDI GCAATG 1 cut(s) 379
BsrFI RCCGGY 1 cut(s) 812
BsrGI TGTACA 1 cut(s) 363
BsrI ACTGG 3 cut(s) 107, 341, 841
BssAI RCCGGY 1 cut(s) 812
BssECI CCNNGG 2 cut(s) 595, 891
BssHII GCGCGC 1 cut(s) 868
BssMI GATC 3 cut(s) 213, 603, 909
Bst2UI CCWGG 3 cut(s) 597, 732, 907
Bst4CI ACNGT 2 cut(s) 44, 73
BstAUI TGTACA 1 cut(s) 363
BstBAI YACGTR 1 cut(s) 1013
BstC8I GCNNGC 5 cut(s) 284, 737, 781, 814, 870
BstDEI CTNAG 2 cut(s) 174, 995
BstDSI CCRYGG 1 cut(s) 891
BstF5I GGATG 1 cut(s) 383
BstFNI CGCG 2 cut(s) 870, 872
BstHHI GCGC 3 cut(s) 704, 870, 872
BstKTI GATC 3 cut(s) 216, 606, 912
BstMAI GTCTC 2 cut(s) 618, 963
BstMBI GATC 3 cut(s) 213, 603, 909
BstMWI GCNNNNNNNGC 3 cut(s) 699, 736, 874
BstNI CCWGG 3 cut(s) 597, 732, 907
BstNSI RCATGY 1 cut(s) 160
BstSCI CCNGG 3 cut(s) 595, 730, 905
BstSFI CTRYAG 1 cut(s) 919
BstSLI GKGCMC 1 cut(s) 525
BstUI CGCG 2 cut(s) 870, 872
BstV1I GCAGC 2 cut(s) 147, 356
BstV2I GAAGAC 2 cut(s) 582, 712
Bsu36I CCTNAGG 1 cut(s) 174
BsuRI GGCC 8 cut(s) 209, 432, 523, 623, 666, 710, 898, 969
BtgI CCRYGG 1 cut(s) 891
BtrI CACGTC 1 cut(s) 279
BtsCI GGATG 1 cut(s) 383
BtsI GCAGTG 1 cut(s) 195
BtsIMutI CAGTG 1 cut(s) 195
Cac8I GCNNGC 5 cut(s) 284, 737, 781, 814, 870
CfoI GCGC 3 cut(s) 704, 870, 872
Cfr10I RCCGGY 1 cut(s) 812
Cfr13I GGNCC 7 cut(s) 44, 431, 521, 522, 709, 896, 968
Csp6I GTAC 8 cut(s) 40, 127, 364, 480, 631, 656, 838, 1010
CviAII CATG 5 cut(s) 157, 393, 546, 913, 937
CviQI GTAC 8 cut(s) 40, 127, 364, 480, 631, 656, 838, 1010
DdeI CTNAG 2 cut(s) 174, 995
DpnI GATC 3 cut(s) 215, 605, 911
DpnII GATC 3 cut(s) 213, 603, 909
EciI GGCGGA 1 cut(s) 931
Eco147I AGGCCT 1 cut(s) 209
Eco24I GRGCYC 1 cut(s) 525
Eco31I GGTCTC 1 cut(s) 963
Eco47I GGWCC 1 cut(s) 44
Eco81I CCTNAGG 1 cut(s) 174
Eco88I CYCGRG 1 cut(s) 197
EcoO109I RGGNCCY 3 cut(s) 521, 522, 896
EcoRII CCWGG 3 cut(s) 595, 730, 905
EcoT22I ATGCAT 1 cut(s) 394
EcoT38I GRGCYC 1 cut(s) 525
FaeI CATG 5 cut(s) 160, 396, 549, 916, 940
FaiI YATR 9 cut(s) 131, 158, 390, 394, 528, 547, 761, 914, 938
FalI AAGNNNNNCTT 4 cut(s) 194, 226, 635, 667
FaqI GGGAC 1 cut(s) 155
FatI CATG 5 cut(s) 156, 392, 545, 912, 936
FauI CCCGC 2 cut(s) 275, 575
FbaI TGATCA 1 cut(s) 213
Fnu4HI GCNGC 6 cut(s) 161, 181, 244, 370, 691, 967
FokI GGATG 1 cut(s) 390
FriOI GRGCYC 1 cut(s) 525
Fsp4HI GCNGC 6 cut(s) 161, 181, 244, 370, 691, 967
FspBI CTAG 1 cut(s) 483
GlaI GCGC 3 cut(s) 703, 869, 871
GluI GCNGC 6 cut(s) 161, 181, 244, 370, 691, 967
HaeIII GGCC 8 cut(s) 209, 432, 523, 623, 666, 710, 898, 969
HapII CCGG 1 cut(s) 813
HhaI GCGC 3 cut(s) 704, 870, 872
Hin1II CATG 5 cut(s) 160, 396, 549, 916, 940
Hin6I GCGC 3 cut(s) 702, 868, 870
HinP1I GCGC 3 cut(s) 702, 868, 870
HinfI GANTC 6 cut(s) 383, 443, 552, 611, 638, 767
HpaII CCGG 1 cut(s) 813
HphI GGTGA 5 cut(s) 164, 256, 715, 776, 877
Hpy188I TCNGA 2 cut(s) 58, 276
Hpy188III TCNNGA 2 cut(s) 199, 355
Hpy99I CGWCG 1 cut(s) 803
HpyAV CCTTC 4 cut(s) 196, 512, 656, 779
HpyCH4III ACNGT 2 cut(s) 44, 73
HpyCH4IV ACGT 6 cut(s) 278, 303, 321, 478, 926, 1012
HpyCH4V TGCA 4 cut(s) 92, 372, 392, 407
HpyF10VI GCNNNNNNNGC 3 cut(s) 699, 736, 874
HpyF3I CTNAG 2 cut(s) 174, 995
HpySE526I ACGT 6 cut(s) 278, 303, 321, 478, 926, 1012
Hsp92II CATG 5 cut(s) 160, 396, 549, 916, 940
HspAI GCGC 3 cut(s) 702, 868, 870
KroI GCCGGC 1 cut(s) 812
KroNI GCCGGC 1 cut(s) 814
Ksp22I TGATCA 1 cut(s) 213
Kzo9I GATC 3 cut(s) 213, 603, 909
LmnI GCTCC 2 cut(s) 794, 882
Lsp1109I GCAGC 2 cut(s) 147, 356
LweI GCATC 1 cut(s) 551
MaeI CTAG 1 cut(s) 483
MaeII ACGT 6 cut(s) 278, 303, 321, 478, 926, 1012
MaeIII GTNAC 3 cut(s) 76, 152, 764
MalI GATC 3 cut(s) 215, 605, 911
MboI GATC 3 cut(s) 213, 603, 909
MboII GAAGA 5 cut(s) 338, 527, 587, 643, 712
MhlI GDGCHC 1 cut(s) 525
MluCI AATT 1 cut(s) 93
MlyI GAGTC 4 cut(s) 561, 620, 632, 761
MmeI TCCRAC 2 cut(s) 254, 795
Mph1103I ATGCAT 1 cut(s) 394
MroNI GCCGGC 1 cut(s) 812
MseI TTAA 2 cut(s) 402, 627
MslI CAYNNNNRTG 2 cut(s) 74, 890
MspA1I CMGCKG 2 cut(s) 269, 369
MspI CCGG 1 cut(s) 813
MspR9I CCNGG 3 cut(s) 597, 732, 907
Mva1269I GAATGC 1 cut(s) 52
MvaI CCWGG 3 cut(s) 597, 732, 907
MvnI CGCG 2 cut(s) 870, 872
MwoI GCNNNNNNNGC 3 cut(s) 699, 736, 874
NaeI GCCGGC 1 cut(s) 814
NdeII GATC 3 cut(s) 213, 603, 909
NgoMIV GCCGGC 1 cut(s) 812
NlaIII CATG 5 cut(s) 160, 396, 549, 916, 940
NlaIV GGNNCC 7 cut(s) 468, 523, 790, 834, 878, 897, 970
NmuCI GTSAC 3 cut(s) 76, 152, 764
NsiI ATGCAT 1 cut(s) 394
NspI RCATGY 1 cut(s) 160
OliI CACNNNNGTG 2 cut(s) 74, 890
PauI GCGCGC 1 cut(s) 868
PceI AGGCCT 1 cut(s) 209
PcsI WCGNNNNNNNCGW 2 cut(s) 33, 1009
PctI GAATGC 1 cut(s) 52
PdiI GCCGGC 1 cut(s) 814
PfeI GAWTC 2 cut(s) 383, 443
PkrI GCNGC 6 cut(s) 162, 182, 245, 371, 692, 968
PleI GAGTC 4 cut(s) 560, 619, 632, 761
PpsI GAGTC 4 cut(s) 560, 619, 632, 761
Ppu21I YACGTR 1 cut(s) 1013
Psp1406I AACGTT 1 cut(s) 321
Psp6I CCWGG 3 cut(s) 595, 730, 905
PspGI CCWGG 3 cut(s) 595, 730, 905
PspN4I GGNNCC 7 cut(s) 468, 523, 790, 834, 878, 897, 970
PspOMI GGGCCC 1 cut(s) 521
PspPI GGNCC 7 cut(s) 44, 431, 521, 522, 709, 896, 968
PteI GCGCGC 1 cut(s) 868
PvuII CAGCTG 1 cut(s) 369
RsaI GTAC 8 cut(s) 41, 128, 365, 481, 632, 657, 839, 1011
RsaNI GTAC 8 cut(s) 40, 127, 364, 480, 631, 656, 838, 1010
RseI CAYNNNNRTG 2 cut(s) 74, 890
SaqAI TTAA 2 cut(s) 402, 627
SatI GCNGC 6 cut(s) 161, 181, 244, 370, 691, 967
Sau3AI GATC 3 cut(s) 213, 603, 909
Sau96I GGNCC 7 cut(s) 44, 431, 521, 522, 709, 896, 968
SchI GAGTC 4 cut(s) 561, 620, 632, 761
ScrFI CCNGG 3 cut(s) 597, 732, 907
SduI GDGCHC 1 cut(s) 525
SfaNI GCATC 1 cut(s) 551
SfcI CTRYAG 1 cut(s) 919
SinI GGWCC 1 cut(s) 44
SmiMI CAYNNNNRTG 2 cut(s) 74, 890
SmlI CTYRAG 2 cut(s) 617, 827
SmoI CTYRAG 2 cut(s) 617, 827
Sse9I AATT 1 cut(s) 93
SseBI AGGCCT 1 cut(s) 209
SspMI CTAG 1 cut(s) 483
StuI AGGCCT 1 cut(s) 209
StyD4I CCNGG 3 cut(s) 595, 730, 905
TaaI ACNGT 2 cut(s) 44, 73
TaiI ACGT 6 cut(s) 281, 306, 324, 481, 929, 1015
TaqI TCGA 6 cut(s) 36, 123, 609, 678, 801, 901
TasI AATT 1 cut(s) 93
TatI WGTACW 3 cut(s) 126, 363, 630
TauI GCSGC 4 cut(s) 183, 246, 693, 969
TfiI GAWTC 2 cut(s) 383, 443
Tru1I TTAA 2 cut(s) 402, 627
Tru9I TTAA 2 cut(s) 402, 627
TscAI CASTG 1 cut(s) 195
TseFI GTSAC 3 cut(s) 76, 152, 764
TseI GCWGC 2 cut(s) 160, 369
Tsp45I GTSAC 3 cut(s) 76, 152, 764
TspGWI ACGGA 1 cut(s) 298
TspRI CASTG 1 cut(s) 195
VpaK11BI GGWCC 1 cut(s) 44
XceI RCATGY 1 cut(s) 160
XcmI CCANNNNNNNNNTGG 1 cut(s) 597
XspI CTAG 1 cut(s) 483
Zsp2I ATGCAT 1 cut(s) 394
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.