pycom17g24530

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0012548
Physical Location & Seq
Forward (+)
22807537 .. 22812053
4517 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 975 bp
ATGAGAGGGGTGGCGAGGAGCTGCGTCCAATCAATGCTCAAGTTGGTGAATTGTATGATTGGGATGGTAGGGCTGGCCATGATTATGTACTCGTTGTGGTTGATTAGCGCGTGGCAGAGGAACATGCCTCACTTCCCCCCACCACTCGACCACTCTCACCACCATCCTACACCTTGGTTCATATACACTTTTCTTGGCATTGGGATCACTCTGCTTGTGATCACATGCTCAGGTCATATCGCCGCTGACACTGCAAATGGTTGTTGCCTTTATTTGTACATGGTGTTTGTCTTCTTACTCTTCATGCTTGAAGCCGGAGTGACTGCTGACATATTTTTAAACCGTGACTGGGAAGAGGACTTTCCAGATGATCCAACTAGGAGATTTGATCAATTCAAAGAATTTGTAAGGTCGAACTTTGATATCTGCAAATGGATTGGCTTGTCAATAGTTTGTGTACAGGGGCTGTCTTTGTTATTGGCAATGATACTCAAAGCTCTTGGGCCACATCCGTATTATGATAGTGATGATGAGTATATTCCTGAGAGGGTTACACTCCTAAACAATGCTGTTCTTCCACCTCCTTATGTCGTTGGTGACCCTGTATATGGTTCCAAAAGTGATTCATGGAACATAAGGATCAATGACAAGGTAGGAATGTTAGATTTTTTGAATGGGCCATGTTATATATCTGCGGCATTATTTCTTTACTTTTTTAAATCAAAGATCTATATTTCGTTTCTCATAATTTATAAAACTCTGTTATTACGTTTTCTTCTGTCTTGTTCTAATTCTTGTGCCTTAATCTTTGTTACTACTTTTCACTGGAGTATTTATAAAAAAGAAAAGGATCCTCTATCTGATTCTTTCATGAGTCATTCAGATTCTGTAGGACAGCTGAGAGTACAAACAGGTAAATTGATTGCTCAGCTGGGCTTCTACTTGTGGATGGATGGTAGTGTGGAGACACAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tetraspannin

Protein Analysis

325

Amino Acids

36.98

Weight (kDa)

6.12

Isoelectric Point (pI)

33.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tetraspanin PF00335 11 - 117 3e-11 Tetraspanin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 753, 837
AccII CGCG 1 cut(s) 110
AciI CCGC 2 cut(s) 243, 695
AclWI GGATC 5 cut(s) 212, 365, 647, 845, 858
AcoI YGGCCR 1 cut(s) 75
AcsI RAATTY 1 cut(s) 401
AfaI GTAC 4 cut(s) 89, 278, 459, 906
AfiI CCNNNNNNNGG 2 cut(s) 349, 608
AgsI TTSAA 3 cut(s) 311, 397, 673
AluBI AGCT 4 cut(s) 21, 497, 898, 931
AluI AGCT 4 cut(s) 21, 497, 898, 931
Alw26I GTCTC 1 cut(s) 959
AlwI GGATC 5 cut(s) 212, 365, 647, 845, 858
AlwNI CAGNNNCTG 2 cut(s) 466, 887
AoxI GGCC 3 cut(s) 75, 503, 677
ApeKI GCWGC 1 cut(s) 21
ApoI RAATTY 1 cut(s) 401
AspLEI GCGC 1 cut(s) 110
AspS9I GGNCC 2 cut(s) 503, 677
AsuHPI GGTGA 3 cut(s) 58, 149, 608
BalI TGGCCA 1 cut(s) 77
BamHI GGATCC 1 cut(s) 850
BbsI GAAGAC 1 cut(s) 283
BbvI GCAGC 1 cut(s) 8
BccI CCATC 4 cut(s) 58, 171, 943, 947
BclI TGATCA 2 cut(s) 219, 388
BcoDI GTCTC 1 cut(s) 959
BfaI CTAG 1 cut(s) 378
BfmI CTRYAG 1 cut(s) 888
BglII AGATCT 1 cut(s) 726
BisI GCNGC 3 cut(s) 22, 243, 696
BlpI GCTNAGC 1 cut(s) 927
BlsI GCNGC 3 cut(s) 23, 244, 697
BmgT120I GGNCC 2 cut(s) 503, 677
BmiI GGNNCC 2 cut(s) 613, 852
BmrI ACTGGG 1 cut(s) 358
BmuI ACTGGG 1 cut(s) 358
BpiI GAAGAC 1 cut(s) 283
BpmI CTGGAG 1 cut(s) 847
Bpu10I CCTNAGC 1 cut(s) 229
Bpu1102I GCTNAGC 1 cut(s) 927
BpuEI CTTGAG 1 cut(s) 23
BsaJI CCNNGG 1 cut(s) 173
Bsc4I CCNNNNNNNGG 2 cut(s) 349, 608
Bse1I ACTGG 2 cut(s) 353, 830
Bse3DI GCAATG 1 cut(s) 489
BseDI CCNNGG 1 cut(s) 173
BseGI GGATG 5 cut(s) 69, 163, 508, 954, 958
BseLI CCNNNNNNNGG 2 cut(s) 349, 608
BseMI GCAATG 1 cut(s) 489
BseMII CTCAG 4 cut(s) 243, 534, 890, 941
BseNI ACTGG 2 cut(s) 353, 830
BseRI GAGGAG 1 cut(s) 31
BseXI GCAGC 1 cut(s) 8
BseYI CCCAGC 1 cut(s) 931
Bsh1236I CGCG 1 cut(s) 110
BshFI GGCC 3 cut(s) 77, 505, 679
BsiSI CCGG 1 cut(s) 315
BslI CCNNNNNNNGG 2 cut(s) 349, 608
BsmAI GTCTC 1 cut(s) 959
BsnI GGCC 3 cut(s) 77, 505, 679
Bsp1407I TGTACA 2 cut(s) 276, 457
Bsp143I GATC 7 cut(s) 204, 219, 370, 388, 639, 726, 850
Bsp1720I GCTNAGC 1 cut(s) 927
BspACI CCGC 2 cut(s) 243, 695
BspANI GGCC 3 cut(s) 77, 505, 679
BspCNI CTCAG 4 cut(s) 242, 535, 891, 940
BspFNI CGCG 1 cut(s) 110
BspHI TCATGA 1 cut(s) 870
BspLI GGNNCC 2 cut(s) 613, 852
BspPI GGATC 5 cut(s) 212, 365, 647, 845, 858
BsrDI GCAATG 1 cut(s) 489
BsrGI TGTACA 2 cut(s) 276, 457
BsrI ACTGG 2 cut(s) 353, 830
BssECI CCNNGG 1 cut(s) 173
BssMI GATC 7 cut(s) 204, 219, 370, 388, 639, 726, 850
BssT1I CCWWGG 1 cut(s) 173
Bst4CI ACNGT 1 cut(s) 344
Bst6I CTCTTC 2 cut(s) 305, 348
BstAUI TGTACA 2 cut(s) 276, 457
BstC8I GCNNGC 1 cut(s) 75
BstDEI CTNAG 4 cut(s) 229, 543, 899, 927
BstEII GGTNACC 1 cut(s) 596
BstF5I GGATG 5 cut(s) 69, 163, 508, 954, 958
BstFNI CGCG 1 cut(s) 110
BstHHI GCGC 1 cut(s) 110
BstKTI GATC 7 cut(s) 207, 222, 373, 391, 642, 729, 853
BstMAI GTCTC 1 cut(s) 959
BstMBI GATC 7 cut(s) 204, 219, 370, 388, 639, 726, 850
BstMWI GCNNNNNNNGC 1 cut(s) 251
BstNSI RCATGY 2 cut(s) 127, 228
BstPI GGTNACC 1 cut(s) 596
BstSFI CTRYAG 1 cut(s) 888
BstUI CGCG 1 cut(s) 110
BstV1I GCAGC 1 cut(s) 8
BstV2I GAAGAC 1 cut(s) 283
BstX2I RGATCY 2 cut(s) 726, 850
BstYI RGATCY 2 cut(s) 726, 850
BsuRI GGCC 3 cut(s) 77, 505, 679
BtsCI GGATG 5 cut(s) 69, 163, 508, 954, 958
BtsI GCAGTG 1 cut(s) 249
BtsIMutI CAGTG 2 cut(s) 249, 823
Cac8I GCNNGC 1 cut(s) 75
CaiI CAGNNNCTG 2 cut(s) 466, 887
CciI TCATGA 1 cut(s) 870
CfoI GCGC 1 cut(s) 110
Cfr13I GGNCC 2 cut(s) 503, 677
CseI GACGC 1 cut(s) 13
Csp6I GTAC 4 cut(s) 88, 277, 458, 905
CviAII CATG 8 cut(s) 79, 124, 225, 280, 304, 627, 681, 871
CviQI GTAC 4 cut(s) 88, 277, 458, 905
DdeI CTNAG 4 cut(s) 229, 543, 899, 927
DpnI GATC 7 cut(s) 206, 221, 372, 390, 641, 728, 852
DpnII GATC 7 cut(s) 204, 219, 370, 388, 639, 726, 850
DraI TTTAAA 2 cut(s) 339, 718
EaeI YGGCCR 1 cut(s) 75
Eam1104I CTCTTC 2 cut(s) 305, 348
EarI CTCTTC 2 cut(s) 305, 348
Eco130I CCWWGG 1 cut(s) 173
Eco32I GATATC 1 cut(s) 424
Eco91I GGTNACC 1 cut(s) 596
EcoO65I GGTNACC 1 cut(s) 596
EcoRV GATATC 1 cut(s) 424
EcoT14I CCWWGG 1 cut(s) 173
ErhI CCWWGG 1 cut(s) 173
FaeI CATG 8 cut(s) 82, 127, 228, 283, 307, 630, 684, 874
FatI CATG 8 cut(s) 78, 123, 224, 279, 303, 626, 680, 870
FbaI TGATCA 2 cut(s) 219, 388
Fnu4HI GCNGC 3 cut(s) 22, 243, 696
FokI GGATG 5 cut(s) 76, 150, 495, 961, 965
Fsp4HI GCNGC 3 cut(s) 22, 243, 696
FspBI CTAG 1 cut(s) 378
GlaI GCGC 1 cut(s) 109
GluI GCNGC 3 cut(s) 22, 243, 696
GsaI CCCAGC 1 cut(s) 935
GsuI CTGGAG 1 cut(s) 847
HaeIII GGCC 3 cut(s) 77, 505, 679
HapII CCGG 1 cut(s) 315
HgaI GACGC 1 cut(s) 13
HhaI GCGC 1 cut(s) 110
Hin1II CATG 8 cut(s) 82, 127, 228, 283, 307, 630, 684, 874
Hin6I GCGC 1 cut(s) 108
HinP1I GCGC 1 cut(s) 108
HinfI GANTC 4 cut(s) 623, 863, 874, 884
HpaII CCGG 1 cut(s) 315
HphI GGTGA 3 cut(s) 58, 149, 608
Hpy166II GTNNAC 1 cut(s) 458
Hpy188I TCNGA 2 cut(s) 862, 883
Hpy188III TCNNGA 3 cut(s) 365, 542, 871
Hpy8I GTNNAC 1 cut(s) 458
HpyCH4III ACNGT 1 cut(s) 344
HpyCH4IV ACGT 1 cut(s) 769
HpyCH4V TGCA 2 cut(s) 254, 429
HpyF10VI GCNNNNNNNGC 1 cut(s) 251
HpyF3I CTNAG 4 cut(s) 229, 543, 899, 927
HpySE526I ACGT 1 cut(s) 769
Hsp92II CATG 8 cut(s) 82, 127, 228, 283, 307, 630, 684, 874
HspAI GCGC 1 cut(s) 108
Ksp22I TGATCA 2 cut(s) 219, 388
Kzo9I GATC 7 cut(s) 204, 219, 370, 388, 639, 726, 850
LmnI GCTCC 1 cut(s) 18
Lsp1109I GCAGC 1 cut(s) 8
MaeI CTAG 1 cut(s) 378
MaeII ACGT 1 cut(s) 769
MaeIII GTNAC 5 cut(s) 319, 344, 550, 596, 811
MalI GATC 7 cut(s) 206, 221, 372, 390, 641, 728, 852
MboI GATC 7 cut(s) 204, 219, 370, 388, 639, 726, 850
MboII GAAGA 5 cut(s) 283, 292, 365, 566, 767
MflI RGATCY 2 cut(s) 726, 850
MlsI TGGCCA 1 cut(s) 77
MluCI AATT 6 cut(s) 49, 392, 401, 747, 790, 917
MluNI TGGCCA 1 cut(s) 77
MlyI GAGTC 1 cut(s) 883
MmeI TCCRAC 1 cut(s) 398
MnlI CCTC 7 cut(s) 9, 111, 138, 349, 540, 591, 864
Mox20I TGGCCA 1 cut(s) 77
MscI TGGCCA 1 cut(s) 77
MseI TTAA 3 cut(s) 338, 717, 803
MslI CAYNNNNRTG 1 cut(s) 83
Msp20I TGGCCA 1 cut(s) 77
MspA1I CMGCKG 3 cut(s) 245, 898, 931
MspI CCGG 1 cut(s) 315
MvnI CGCG 1 cut(s) 110
MwoI GCNNNNNNNGC 1 cut(s) 251
NdeII GATC 7 cut(s) 204, 219, 370, 388, 639, 726, 850
NlaIII CATG 8 cut(s) 82, 127, 228, 283, 307, 630, 684, 874
NlaIV GGNNCC 2 cut(s) 613, 852
NmuCI GTSAC 3 cut(s) 319, 344, 596
NspI RCATGY 2 cut(s) 127, 228
PagI TCATGA 1 cut(s) 870
PfeI GAWTC 3 cut(s) 623, 863, 884
PkrI GCNGC 3 cut(s) 23, 244, 697
PleI GAGTC 1 cut(s) 882
PpsI GAGTC 1 cut(s) 882
PsiI TTATAA 2 cut(s) 753, 837
PspEI GGTNACC 1 cut(s) 596
PspFI CCCAGC 1 cut(s) 931
PspN4I GGNNCC 2 cut(s) 613, 852
PspPI GGNCC 2 cut(s) 503, 677
PstNI CAGNNNCTG 2 cut(s) 466, 887
PsuI RGATCY 2 cut(s) 726, 850
PvuII CAGCTG 2 cut(s) 898, 931
RsaI GTAC 4 cut(s) 89, 278, 459, 906
RsaNI GTAC 4 cut(s) 88, 277, 458, 905
RseI CAYNNNNRTG 1 cut(s) 83
SaqAI TTAA 3 cut(s) 338, 717, 803
SatI GCNGC 3 cut(s) 22, 243, 696
Sau3AI GATC 7 cut(s) 204, 219, 370, 388, 639, 726, 850
Sau96I GGNCC 2 cut(s) 503, 677
SchI GAGTC 1 cut(s) 883
SfcI CTRYAG 1 cut(s) 888
SmiMI CAYNNNNRTG 1 cut(s) 83
SmlI CTYRAG 1 cut(s) 38
SmoI CTYRAG 1 cut(s) 38
Sse9I AATT 6 cut(s) 49, 392, 401, 747, 790, 917
SsiI CCGC 2 cut(s) 243, 695
SspMI CTAG 1 cut(s) 378
StyI CCWWGG 1 cut(s) 173
TaaI ACNGT 1 cut(s) 344
TaiI ACGT 1 cut(s) 772
TaqI TCGA 2 cut(s) 147, 413
TasI AATT 6 cut(s) 49, 392, 401, 747, 790, 917
TatI WGTACW 4 cut(s) 87, 276, 457, 904
TauI GCSGC 2 cut(s) 245, 698
TfiI GAWTC 3 cut(s) 623, 863, 884
Tru1I TTAA 3 cut(s) 338, 717, 803
Tru9I TTAA 3 cut(s) 338, 717, 803
TscAI CASTG 2 cut(s) 256, 830
TseFI GTSAC 3 cut(s) 319, 344, 596
TseI GCWGC 1 cut(s) 21
Tsp45I GTSAC 3 cut(s) 319, 344, 596
TspDTI ATGAA 4 cut(s) 169, 292, 615, 859
TspGWI ACGGA 1 cut(s) 501
TspRI CASTG 2 cut(s) 256, 830
XapI RAATTY 1 cut(s) 401
XceI RCATGY 2 cut(s) 127, 228
XspI CTAG 1 cut(s) 378
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.