pycom17g25400

Protein PLASTID MOVEMENT IMPAIRED

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0007774
Physical Location & Seq
Forward (+)
23493583 .. 23494068
486 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 486 bp
ATGGATGTTATAAGAAATGAGCTTAAGCATGTAAGAGACAAGACAGCTCGGTTAAGGAAGACAGAAGAGAAATCGGATTTGAGTGTTCAAAATCTCAACTCAAAGCTTCTCCGAGCAAAAGTCAAGTTGGAAGCTGTATCTGCATCCGAAGAGAAAGCAAAATCGATCGCATCCAATCTATCTCTGACTTTTGACAAGCTCAAGACAGAAGGAGAAGCTGCAAAGAAAGAAAAGGAGCTTGTCTGCGAAGAGACTGCAAGCATCAACTCCGAAATTATGAAAATGGAATCAGAGATAGAATCAACCGAGGAAAAGTTACCGTCTACAACGCAAGAGCTCGAAACAGTCAAATCATCTGAAGCTGTAGCGCTTGAAAATCTCAAAACTCTGATTGAGAACACCGTACAAGCTAGAGCTTTTCAGTCGCAGAGTAGTTCTTCAATTACCATATCCAAGTTTGAACACAAGTACGTAACTGGACTTTGA

Protein Analysis

162

Amino Acids

17.99

Weight (kDa)

5.87

Isoelectric Point (pI)

51.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WEMBL PF05701 4 - 156 3e-10 Weak chloroplast movement under blue light
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 11
AccI GTMKAC 1 cut(s) 323
AcuI CTGAAG 1 cut(s) 378
AfaI GTAC 2 cut(s) 405, 470
AfeI AGCGCT 1 cut(s) 369
AflII CTTAAG 1 cut(s) 23
AgsI TTSAA 4 cut(s) 89, 374, 441, 461
Alw21I GWGCWC 1 cut(s) 339
Alw26I GTCTC 2 cut(s) 30, 245
Aor51HI AGCGCT 1 cut(s) 369
ApeKI GCWGC 1 cut(s) 218
AspLEI GCGC 1 cut(s) 370
BanII GRGCYC 1 cut(s) 339
BbsI GAAGAC 1 cut(s) 65
Bbv12I GWGCWC 1 cut(s) 339
BbvI GCAGC 1 cut(s) 205
BcgI CGANNNNNNTGC 2 cut(s) 236, 270
BcoDI GTCTC 2 cut(s) 30, 245
BfaI CTAG 1 cut(s) 411
BfmI CTRYAG 1 cut(s) 363
BfoI RGCGCY 1 cut(s) 371
BfrI CTTAAG 1 cut(s) 23
BisI GCNGC 1 cut(s) 219
BlsI GCNGC 1 cut(s) 220
BmsI GCATC 3 cut(s) 152, 179, 270
BpiI GAAGAC 1 cut(s) 65
BpuEI CTTGAG 1 cut(s) 185
Bsa29I ATCGAT 1 cut(s) 164
BsaAI YACGTR 1 cut(s) 472
BsaJI CCNNGG 1 cut(s) 306
Bse1I ACTGG 1 cut(s) 481
BseCI ATCGAT 1 cut(s) 164
BseDI CCNNGG 1 cut(s) 306
BseGI GGATG 3 cut(s) 10, 143, 170
BseNI ACTGG 1 cut(s) 481
BseXI GCAGC 1 cut(s) 205
Bsh1285I CGRYCG 1 cut(s) 168
BshVI ATCGAT 1 cut(s) 164
BsiEI CGRYCG 1 cut(s) 168
BsiHKAI GWGCWC 1 cut(s) 339
BsmAI GTCTC 2 cut(s) 30, 245
Bsp1286I GDGCHC 1 cut(s) 339
Bsp143I GATC 1 cut(s) 165
BspDI ATCGAT 1 cut(s) 164
BspTI CTTAAG 1 cut(s) 23
BsrI ACTGG 1 cut(s) 481
BssECI CCNNGG 1 cut(s) 306
BssMI GATC 1 cut(s) 165
Bst4CI ACNGT 3 cut(s) 321, 346, 403
Bst6I CTCTTC 3 cut(s) 60, 144, 243
BstAFI CTTAAG 1 cut(s) 23
BstBAI YACGTR 1 cut(s) 472
BstC8I GCNNGC 1 cut(s) 259
BstF5I GGATG 3 cut(s) 10, 143, 170
BstH2I RGCGCY 1 cut(s) 371
BstHHI GCGC 1 cut(s) 370
BstKTI GATC 1 cut(s) 168
BstMAI GTCTC 2 cut(s) 30, 245
BstMBI GATC 1 cut(s) 165
BstMCI CGRYCG 1 cut(s) 168
BstMWI GCNNNNNNNGC 1 cut(s) 140
BstNSI RCATGY 1 cut(s) 32
BstSFI CTRYAG 1 cut(s) 363
BstSNI TACGTA 1 cut(s) 472
BstV1I GCAGC 1 cut(s) 205
BstV2I GAAGAC 1 cut(s) 65
Bsu15I ATCGAT 1 cut(s) 164
BsuTUI ATCGAT 1 cut(s) 164
BtsCI GGATG 3 cut(s) 10, 143, 170
Cac8I GCNNGC 1 cut(s) 259
CfoI GCGC 1 cut(s) 370
ClaI ATCGAT 1 cut(s) 164
Csp6I GTAC 2 cut(s) 404, 469
CviAII CATG 1 cut(s) 29
CviQI GTAC 2 cut(s) 404, 469
DpnI GATC 1 cut(s) 167
DpnII GATC 1 cut(s) 165
Eam1104I CTCTTC 3 cut(s) 60, 144, 243
EarI CTCTTC 3 cut(s) 60, 144, 243
Ecl136II GAGCTC 1 cut(s) 337
Eco105I TACGTA 1 cut(s) 472
Eco24I GRGCYC 1 cut(s) 339
Eco47III AGCGCT 1 cut(s) 369
Eco53kI GAGCTC 1 cut(s) 337
Eco57I CTGAAG 1 cut(s) 378
EcoICRI GAGCTC 1 cut(s) 337
EcoT38I GRGCYC 1 cut(s) 339
FaeI CATG 1 cut(s) 32
FaiI YATR 4 cut(s) 11, 30, 278, 449
FatI CATG 1 cut(s) 28
FblI GTMKAC 1 cut(s) 323
Fnu4HI GCNGC 1 cut(s) 219
FokI GGATG 3 cut(s) 17, 130, 157
FriOI GRGCYC 1 cut(s) 339
Fsp4HI GCNGC 1 cut(s) 219
FspBI CTAG 1 cut(s) 411
GlaI GCGC 1 cut(s) 369
GluI GCNGC 1 cut(s) 219
HaeII RGCGCY 1 cut(s) 371
HhaI GCGC 1 cut(s) 370
Hin1II CATG 1 cut(s) 32
Hin6I GCGC 1 cut(s) 368
HinP1I GCGC 1 cut(s) 368
HindIII AAGCTT 1 cut(s) 104
HinfI GANTC 2 cut(s) 287, 299
Hpy166II GTNNAC 1 cut(s) 324
Hpy188I TCNGA 8 cut(s) 76, 113, 148, 186, 271, 292, 358, 390
Hpy188III TCNNGA 1 cut(s) 202
Hpy8I GTNNAC 1 cut(s) 324
HpyAV CCTTC 1 cut(s) 203
HpyCH4III ACNGT 3 cut(s) 321, 346, 403
HpyCH4IV ACGT 1 cut(s) 471
HpyCH4V TGCA 3 cut(s) 143, 221, 257
HpyF10VI GCNNNNNNNGC 1 cut(s) 140
HpySE526I ACGT 1 cut(s) 471
Hsp92II CATG 1 cut(s) 32
HspAI GCGC 1 cut(s) 368
Kzo9I GATC 1 cut(s) 165
LmnI GCTCC 1 cut(s) 235
LpnPI CCDG 1 cut(s) 462
Lsp1109I GCAGC 1 cut(s) 205
LweI GCATC 3 cut(s) 152, 179, 270
MaeI CTAG 1 cut(s) 411
MaeII ACGT 1 cut(s) 471
MaeIII GTNAC 2 cut(s) 315, 472
MalI GATC 1 cut(s) 167
MboI GATC 1 cut(s) 165
MboII GAAGA 5 cut(s) 70, 77, 161, 260, 429
MhlI GDGCHC 1 cut(s) 339
MluCI AATT 2 cut(s) 273, 441
MmeI TCCRAC 1 cut(s) 108
MnlI CCTC 1 cut(s) 301
MseI TTAA 2 cut(s) 24, 53
MspCI CTTAAG 1 cut(s) 23
MwoI GCNNNNNNNGC 1 cut(s) 140
NdeII GATC 1 cut(s) 165
NlaIII CATG 1 cut(s) 32
NspI RCATGY 1 cut(s) 32
PfeI GAWTC 2 cut(s) 287, 299
PkrI GCNGC 1 cut(s) 220
Ple19I CGATCG 1 cut(s) 168
Ppu21I YACGTR 1 cut(s) 472
PsiI TTATAA 1 cut(s) 11
Psp124BI GAGCTC 1 cut(s) 339
PvuI CGATCG 1 cut(s) 168
RsaI GTAC 2 cut(s) 405, 470
RsaNI GTAC 2 cut(s) 404, 469
SacI GAGCTC 1 cut(s) 339
SaqAI TTAA 2 cut(s) 24, 53
SatI GCNGC 1 cut(s) 219
Sau3AI GATC 1 cut(s) 165
SduI GDGCHC 1 cut(s) 339
SfaNI GCATC 3 cut(s) 152, 179, 270
SfcI CTRYAG 1 cut(s) 363
SmlI CTYRAG 2 cut(s) 23, 200
SmoI CTYRAG 2 cut(s) 23, 200
SnaBI TACGTA 1 cut(s) 472
Sse9I AATT 2 cut(s) 273, 441
SspMI CTAG 1 cut(s) 411
SstI GAGCTC 1 cut(s) 339
TaaI ACNGT 3 cut(s) 321, 346, 403
TaiI ACGT 1 cut(s) 474
TaqI TCGA 2 cut(s) 164, 339
TasI AATT 2 cut(s) 273, 441
TfiI GAWTC 2 cut(s) 287, 299
Tru1I TTAA 2 cut(s) 24, 53
Tru9I TTAA 2 cut(s) 24, 53
TseI GCWGC 1 cut(s) 218
TspDTI ATGAA 1 cut(s) 293
Vha464I CTTAAG 1 cut(s) 23
XceI RCATGY 1 cut(s) 32
XmiI GTMKAC 1 cut(s) 323
XspI CTAG 1 cut(s) 411
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.