pycom17g25880

Belongs to the PI3 PI4-kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0012264
Physical Location & Seq
Reverse (-)
24211044 .. 24227690
16647 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3048 bp
ATGTACACTCTTCCCTTATCTGGAATTGAGAGCTACTTGTTTCAGATATGTTACATGTCAGTGCACAAGCCTAGCCCGTCTTTGGATAAGTTTGTTATTGATATGTGTTCTAAGTCGCTTAAGATAGCTCAGAAGGTGCATTGGTTTTTATTGGCAGAGCTTGAAGACTCAGATGATAATGAAGGAATTAGTAGAATTCAGGAGAAATGTCAGATTGCGGCCACTTTGATGGGTGAGTGGCCGCCTTTGGTACGTCCTCAAAACGAGTCAGCTAGCCCTGGGAGCAAGAACCAGGTATTGAATAGAATATTGTCATCAAAACAGAAATTGTTTTCGCTAACGTCTTCACCACCTGCCCAGAGGTCGTTTTCTTTCTCGCCTTCATCGGTAAACAATTTGCAAGAGGATGGTGGTCAATTCTCTCCCGATGAGAACAAAATTTTTAAAAAATTCATACCGGGGCCAAAGGTTCGGGATGCATTGCTCTTTAGGAAGTCAATGGAGAAAGGTGACGATGACTCTGAAAAAGATGGATTCTTTAAGAGGCTTTTAAGAGACAGTAGAGGTGATGATGAGACAGGTTCTAAGATTAGAGATTCTTTGCCTTCTAGAAAGTCCTCAGAGAAGGATGATGATGATGCCGAGAAGGATGGCTTCTTTAAGAGGCTATCGAGGGATAGTAAAGGTGACGATGAGGAATTGACAGCAAGCTCAGAGGGTTTTTTAAAGAGATTGTTTCGTGATAGGAAGAGTGATTCTGACGATAAATCAATCTCTAAATCAGTGAAAGATGAAGAAAAAGAAGGGTTCTTTCGGAAGTTTTTCAAAGAGAAGTTTGAGGACAAAAAGGACCGGATTGATAAGAATGAAGATGATGATGCACCATACTCTGAAGAAAAAGGTTTGAAATCTGCTGAAGATGATGAAAAAGAGGGGTTCTTCCGAAAAATTTTCAGAGATAAGTTTGATGACAAGAAAGATGGGAATGATAAAATCGAGGAAGGGAGTGCCAATGGAGACGAAGAAGAGCCTTCTGACTTTCCATTGTTTCGAAGATTGTTCCGTGTGCACCCTGAAGATGCAAAAAGTACTGTTGCAAATGAAAACAGCAGTACGGGTGGCTTGCTAGAAAGTAGTCCAGGGACTGAGAACTTTTTTCGCAAATTGTTTAGAGACCGTGATCGTTCAGTTGAAGACTCAGAGCTATTTGGTTCAAGAAAACACAAAGGGAAATGCCCTGGTTCTCCAAAGCAACAAAATGAAAAGTCAAGTGCTAAACCCCCACTTCCAAATGCTACAGCATCCCAATATCGTAAAGGGGCTTATCATGAGTCGCTGGACTTTGTGCAGTCACTATGTGAGACATCTTATGGCTTAGTGGATATATTTCCAATTGAGGATTGCAAGAGTGCTCTTCATGAGTCACTTGCAGAGATCAATTTGCACATTGCTGAGGCTCAAAGTAGTGGAGGAGTTTGCTTTCCAATGGGGAAGGGGATGTATCGCGTGGTTTATATACCGGAAGATGAAGCTGTTCTTTTGAATTCTAGGGAAAAGGCACCGTATCTCATCTGTGTTGAAGTCCTGAAAAGTGAAATTCCTGGCAATGCAAAGGATGCATCTGGTTCTCAAAAGCTTTCTCGAGGAGGAATTCCTTTAGCAAATGGGGATGCACTTCTGCCAAGACCGCCTCCGTGGGCATATCCTTTGTGGACTGTCCAGGAGGTCTATCGCAACAGTAATGATAGGATGTCGAGTTCTACTGCTCAAGCAATTGACCAGGCGATGTCATATACCTCCGAGGCGAAAGTGAAATTTGTTACTGTAAAAATATCTGTGGAGAATAAATTGCATAGTCATACAGTAAAAACAGAGAATATATCAAGGAGTTATCAACGTGGAGAGGCTGCGACTTGTGCACAAGGGAGTGATTTGGAGTGGGTAAGGGTGGTGCTTACAGCAGACCCTGGTGTTAGAATGGAGGACATTGAGGATCAAGGCCCCCTTGGGCGGAAGGAACACCGTCGTGTACCAAGTACAGTGGCTATAGAGGAAGTAAAGGCTGCTGCAGCGAAAGGAGAAGCACCTCCTGGACTTCCTCTGAAAGGGGCTGGTCAGGATTCATCAGATGCACAGCCAATGGTTAATGGGGGTATTCCCAAGGCCAGCAATGCCTTGTCTGGCGAACTTTGGAAGGTAAAGAAGGAGAGAATAAGAAAAGCTTCAATTCATGGAAAGTTACCTGGTTGGGACTTGTGCTCCGTTATTGTGAAGAGTGGTGATGATTGTAGGCAGGAGCATCTTGCTGTGCAACTTATTTCTCACTTTTATGATATATTTCAAGAAGCTGGTCTCCCTCTCTGGTTGCGCCCTTATGAAGTCTTAGTTACTTCTTCTTATACAGCTCTCATTGAAACAATTCCAGATACGGCTTCAATTCATTCTATCAAAAGTAGATATCCCAACATCATGAGCTTGCGAGACTTCTTTGCTGCTAAGTACCAAGAAAATTCTCCTAGTTTTAAACTTGCTCAGAGGAATTTTGTTGAAAGTATGGCTGGATATTCTCTAGTGTGTTACCTTCTGCAGATAAAGGATAGGCACAACGGGAACCTCTTAATGGATGAAGAAGGTCATATTATACATATTGATTTTGGTTTCATGCTCTCCAATTCGCCTGGGGGTGTAAATTTTGAGAGTGCTCCATTCAAATTAACACGTGAACTTCTTGAGGTCATGGATTCTGATGCTGAGGGAGTTCCTAGCGAGTTCTTTGATTATTTTAAGGTTCTGTGCATTCAAGGATTCCTTACATGTCGGAAGCATGCAGAACGCATTATTCTTCTTGTTGAGATGTTGCAGGACTCTGGTTTCCCATGCTTTAAAGGTGGTACACGAACAATACAGAACCTACGGAAAAGATTTCACTTGAATTTGACAGAAGAGCAATGCGTATCCTTGGTGCTTTCGTTGATCAGCAGCAGCTTGGACGCATGGCGAACAAGGCAATATGATTATTATCAGAGGGTTTTGAATGGAATATTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000902 GO:0000904 GO:0003006 GO:0003674 GO:0003824 GO:0004430 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005886 GO:0006629 GO:0006644 GO:0006650 GO:0006793 GO:0006796 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008152 GO:0009653 GO:0009826 GO:0009856 GO:0009860 GO:0009888 GO:0009932 GO:0009987 GO:0010015 GO:0010053 GO:0010054 GO:0012506 GO:0016020 GO:0016043 GO:0016049 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017016 GO:0017137 GO:0019637 GO:0019899 GO:0019900 GO:0019901 GO:0021700 GO:0022414 GO:0022622 GO:0023052 GO:0030154 GO:0030258 GO:0030659 GO:0031090 GO:0031267 GO:0031410 GO:0031982 GO:0032501 GO:0032502 GO:0032989 GO:0035556 GO:0035618 GO:0035619 GO:0040007 GO:0042995 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043424 GO:0044237 GO:0044238 GO:0044255 GO:0044422 GO:0044424 GO:0044433 GO:0044444 GO:0044446 GO:0044463 GO:0044464 GO:0044706 GO:0046486 GO:0046488 GO:0046834 GO:0046854 GO:0048015 GO:0048017 GO:0048364 GO:0048468 GO:0048469 GO:0048588 GO:0048589 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048768 GO:0048856 GO:0048868 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051020 GO:0051704 GO:0051716 GO:0052742 GO:0060560 GO:0065007 GO:0071695 GO:0071704 GO:0071840 GO:0071944 GO:0080147 GO:0090558 GO:0090627 GO:0097708 GO:0099402 GO:0120025 GO:0120038 GO:1905392
KEGG Pathways
Metabolic & Signaling
ko00562 ko01100 ko04070 map00562 map01100 map04070
Pfam Domains
Protein Families
PI3_PI4_kinase

Protein Analysis

1016

Amino Acids

114.08

Weight (kDa)

5.76

Isoelectric Point (pI)

46.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PI3_PI4_kinase PF00454 754 - 962 5.9e-39 Phosphatidylinositol 3- and 4-kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012264)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G09350 AT5G64070 AT5G64070
fragaria_vesca FvH4_1g24240
malus_domestica MD09G1261800.v1.1 MD17G1256600.v1.1
prunus_persica Prupe.3G159200_v2.0.a1 Prupe.3G159200_v2.0.a1 Prupe.3G159200_v2.0.a1
pyrus_communis pycom09g17610 pycom17g25880
rosa_chinensis RchiOBHm_Chr2g0120731
rosa_laevigata RLG00000018575
rosa_multiflora Rmu_sc0000319.1_g000011
rosa_roxburghii Rroxscaffold_2G00123200
rosa_rugosa Rorug02G0230500
rosa_samantha Rh2BG298500 Rh2CG276200
rosa_wichuraiana Rw2G023030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 361
Acc36I ACCTGC 1 cut(s) 361
AccB1I GGYRCC 1 cut(s) 1558
AccII CGCG 1 cut(s) 1506
AciI CCGC 4 cut(s) 218, 242, 1688, 2011
AclWI GGATC 1 cut(s) 2001
AcoI YGGCCR 2 cut(s) 219, 239
AcuI CTGAAG 3 cut(s) 912, 936, 1095
AcvI CACGTG 1 cut(s) 2720
AdeI CACNNNGTG 1 cut(s) 1358
AfaI GTAC 8 cut(s) 5, 252, 1090, 1114, 2031, 2038, 2501, 2893
AfiI CCNNNNNNNGG 5 cut(s) 20, 82, 2010, 2105, 2620
AflII CTTAAG 1 cut(s) 119
AflIII ACRYGT 3 cut(s) 54, 2717, 2813
AleI CACNNNNGTG 1 cut(s) 2025
AloI GAACNNNNNNTCC 2 cut(s) 1741, 1773
Alw21I GWGCWC 6 cut(s) 66, 1071, 1414, 1921, 2261, 2704
Alw26I GTCTC 7 cut(s) 549, 569, 1011, 1167, 1355, 2357, 2475
Alw44I GTGCAC 3 cut(s) 62, 1067, 1917
AlwI GGATC 1 cut(s) 2001
AlwNI CAGNNNCTG 2 cut(s) 1145, 1967
Ama87I CYCGRG 1 cut(s) 1641
AoxI GGCC 5 cut(s) 219, 239, 461, 1999, 2163
ApaLI GTGCAC 3 cut(s) 62, 1067, 1917
ApeKI GCWGC 7 cut(s) 1907, 2063, 2066, 2069, 2492, 2979, 2982
Asp700I GAANNNNTTC 4 cut(s) 821, 1533, 2221, 2418
AspLEI GCGC 1 cut(s) 2370
AspS9I GGNCC 3 cut(s) 461, 850, 2000
AsuC2I CCSGG 1 cut(s) 459
AsuHPI GGTGA 6 cut(s) 245, 339, 521, 578, 698, 2291
AsuII TTCGAA 1 cut(s) 1051
AsuNHI GCTAGC 1 cut(s) 272
AvaI CYCGRG 1 cut(s) 1641
AvaII GGWCC 1 cut(s) 850
BaeGI GKGCMC 3 cut(s) 66, 1071, 1921
BanI GGYRCC 1 cut(s) 1558
BbrPI CACGTG 1 cut(s) 2720
BbsI GAAGAC 3 cut(s) 171, 336, 1200
Bbv12I GWGCWC 6 cut(s) 66, 1071, 1414, 1921, 2261, 2704
BbvCI CCTCAGC 2 cut(s) 1452, 2751
BbvI GCAGC 7 cut(s) 1894, 2050, 2053, 2081, 2479, 2991, 2994
BccI CCATC 5 cut(s) 223, 401, 524, 644, 974
BceAI ACGGC 1 cut(s) 2445
BciVI GTATCC 1 cut(s) 2965
BclI TGATCA 1 cut(s) 2973
BcnI CCSGG 1 cut(s) 459
BcoDI GTCTC 7 cut(s) 549, 569, 1011, 1167, 1355, 2357, 2475
BfaI CTAG 8 cut(s) 72, 273, 609, 1127, 1548, 2517, 2570, 2763
BfmI CTRYAG 4 cut(s) 1296, 2046, 2067, 2585
BfrI CTTAAG 1 cut(s) 119
BfuAI ACCTGC 1 cut(s) 361
BfuI GTATCC 1 cut(s) 2965
BisI GCNGC 9 cut(s) 219, 242, 1908, 2064, 2067, 2070, 2493, 2980, 2983
BlsI GCNGC 9 cut(s) 220, 243, 1909, 2065, 2068, 2071, 2494, 2981, 2984
BmcAI AGTACT 1 cut(s) 1090
Bme18I GGWCC 1 cut(s) 850
BmeT110I CYCGRG 1 cut(s) 1641
BmgT120I GGNCC 3 cut(s) 461, 850, 2000
BmiI GGNNCC 4 cut(s) 462, 1560, 2002, 2612
BmtI GCTAGC 1 cut(s) 276
BpiI GAAGAC 3 cut(s) 171, 336, 1200
Bpu10I CCTNAGC 2 cut(s) 1452, 2751
Bpu14I TTCGAA 1 cut(s) 1051
BpuEI CTTGAG 2 cut(s) 1752, 2750
BpuMI CCSGG 1 cut(s) 459
BsaAI YACGTR 1 cut(s) 2720
BsaBI GATNNNNATC 1 cut(s) 3018
BsaI GGTCTC 2 cut(s) 1167, 2357
BsaWI WCCGGW 2 cut(s) 852, 1519
BsaXI ACNNNNNCTCC 2 cut(s) 2243, 2273
Bsc4I CCNNNNNNNGG 5 cut(s) 20, 82, 2010, 2105, 2620
Bse3DI GCAATG 5 cut(s) 479, 1446, 1612, 2176, 2954
Bse8I GATNNNNATC 1 cut(s) 3018
BseJI GATNNNNATC 1 cut(s) 3018
BseLI CCNNNNNNNGG 5 cut(s) 20, 82, 2010, 2105, 2620
BseMI GCAATG 5 cut(s) 479, 1446, 1612, 2176, 2954
BseMII CTCAG 9 cut(s) 143, 183, 633, 726, 1137, 1212, 1443, 2546, 2742
BseRI GAGGAG 2 cut(s) 1485, 1659
BseSI GKGCMC 3 cut(s) 66, 1071, 1921
BseXI GCAGC 7 cut(s) 1894, 2050, 2053, 2081, 2479, 2991, 2994
BsgI GTGCAG 1 cut(s) 1367
Bsh1236I CGCG 1 cut(s) 1506
BshFI GGCC 5 cut(s) 221, 241, 463, 2001, 2165
BshNI GGYRCC 1 cut(s) 1558
BsiHKAI GWGCWC 6 cut(s) 66, 1071, 1414, 1921, 2261, 2704
BsiHKCI CYCGRG 1 cut(s) 1641
BsiSI CCGG 3 cut(s) 458, 853, 1520
BslFI GGGAC 2 cut(s) 1156, 2264
BslI CCNNNNNNNGG 5 cut(s) 20, 82, 2010, 2105, 2620
BsmAI GTCTC 7 cut(s) 549, 569, 1011, 1167, 1355, 2357, 2475
BsmBI CGTCTC 1 cut(s) 1011
BsmFI GGGAC 2 cut(s) 1156, 2264
BsmI GAATGC 1 cut(s) 2796
BsnI GGCC 5 cut(s) 221, 241, 463, 2001, 2165
Bso31I GGTCTC 2 cut(s) 1167, 2357
BsoBI CYCGRG 1 cut(s) 1641
Bsp119I TTCGAA 1 cut(s) 1051
Bsp1286I GDGCHC 6 cut(s) 66, 1071, 1414, 1921, 2261, 2704
Bsp1407I TGTACA 1 cut(s) 3
Bsp143I GATC 4 cut(s) 1180, 1434, 1993, 2973
BspACI CCGC 4 cut(s) 218, 242, 1688, 2011
BspANI GGCC 5 cut(s) 221, 241, 463, 2001, 2165
BspCNI CTCAG 9 cut(s) 142, 182, 632, 725, 1138, 1211, 1444, 2545, 2743
BspFNI CGCG 1 cut(s) 1506
BspHI TCATGA 3 cut(s) 1327, 1417, 2469
BspLI GGNNCC 4 cut(s) 462, 1560, 2002, 2612
BspMAI CTGCAG 2 cut(s) 2071, 2589
BspMI ACCTGC 1 cut(s) 361
BspOI GCTAGC 1 cut(s) 276
BspPI GGATC 1 cut(s) 2001
BspQI GCTCTTC 3 cut(s) 1020, 1419, 2937
BspT104I TTCGAA 1 cut(s) 1051
BspT107I GGYRCC 1 cut(s) 1558
BspTI CTTAAG 1 cut(s) 119
BspTNI GGTCTC 2 cut(s) 1167, 2357
BsrDI GCAATG 5 cut(s) 479, 1446, 1612, 2176, 2954
BsrGI TGTACA 1 cut(s) 3
BssMI GATC 4 cut(s) 1180, 1434, 1993, 2973
BssT1I CCWWGG 3 cut(s) 2005, 2160, 2958
Bst6I CTCTTC 6 cut(s) 15, 743, 1020, 1419, 2267, 2937
BstAFI CTTAAG 1 cut(s) 119
BstAPI GCANNNNNTGC 1 cut(s) 1616
BstAUI TGTACA 1 cut(s) 3
BstBAI YACGTR 1 cut(s) 2720
BstBI TTCGAA 1 cut(s) 1051
BstC8I GCNNGC 6 cut(s) 274, 709, 1124, 2167, 2477, 2826
BstDSI CCRYGG 1 cut(s) 1694
BstENI CCTNNNNNAGG 1 cut(s) 2103
BstFNI CGCG 1 cut(s) 1506
BstHHI GCGC 1 cut(s) 2370
BstKTI GATC 4 cut(s) 1183, 1437, 1996, 2976
BstMAI GTCTC 7 cut(s) 549, 569, 1011, 1167, 1355, 2357, 2475
BstMBI GATC 4 cut(s) 1180, 1434, 1993, 2973
BstMWI GCNNNNNNNGC 7 cut(s) 282, 1616, 1687, 1916, 2069, 2171, 3004
BstNSI RCATGY 3 cut(s) 58, 2817, 2828
BstSFI CTRYAG 4 cut(s) 1296, 2046, 2067, 2585
BstSLI GKGCMC 3 cut(s) 66, 1071, 1921
BstUI CGCG 1 cut(s) 1506
BstV1I GCAGC 7 cut(s) 1894, 2050, 2053, 2081, 2479, 2991, 2994
BstV2I GAAGAC 3 cut(s) 171, 336, 1200
BstXI CCANNNNNNTGG 1 cut(s) 229
BsuI GTATCC 1 cut(s) 2965
BsuRI GGCC 5 cut(s) 221, 241, 463, 2001, 2165
BtgI CCRYGG 1 cut(s) 1694
BtgZI GCGATG 1 cut(s) 1799
BtsIMutI CAGTG 3 cut(s) 66, 789, 2046
BveI ACCTGC 1 cut(s) 361
Cac8I GCNNGC 6 cut(s) 274, 709, 1124, 2167, 2477, 2826
CaiI CAGNNNCTG 2 cut(s) 1145, 1967
CciI TCATGA 3 cut(s) 1327, 1417, 2469
CfoI GCGC 1 cut(s) 2370
Cfr13I GGNCC 3 cut(s) 461, 850, 2000
CseI GACGC 1 cut(s) 2999
CsiI ACCWGGT 2 cut(s) 291, 2242
Csp6I GTAC 8 cut(s) 4, 251, 1089, 1113, 2030, 2037, 2500, 2892
CspCI CAANNNNNGTGG 2 cut(s) 2022, 2057
CviQI GTAC 8 cut(s) 4, 251, 1089, 1113, 2030, 2037, 2500, 2892
DpnI GATC 4 cut(s) 1182, 1436, 1995, 2975
DpnII GATC 4 cut(s) 1180, 1434, 1993, 2973
DraI TTTAAA 4 cut(s) 445, 726, 2524, 2884
DraIII CACNNNGTG 1 cut(s) 1358
EaeI YGGCCR 2 cut(s) 219, 239
Eam1104I CTCTTC 6 cut(s) 15, 743, 1020, 1419, 2267, 2937
EarI CTCTTC 6 cut(s) 15, 743, 1020, 1419, 2267, 2937
EciI GGCGGA 1 cut(s) 2026
Eco130I CCWWGG 3 cut(s) 2005, 2160, 2958
Eco31I GGTCTC 2 cut(s) 1167, 2357
Eco32I GATATC 1 cut(s) 2459
Eco47I GGWCC 1 cut(s) 850
Eco57I CTGAAG 3 cut(s) 912, 936, 1095
Eco72I CACGTG 1 cut(s) 2720
Eco88I CYCGRG 1 cut(s) 1641
EcoNI CCTNNNNNAGG 1 cut(s) 2103
EcoO109I RGGNCCY 1 cut(s) 2000
EcoRI GAATTC 3 cut(s) 195, 1543, 1650
EcoRV GATATC 1 cut(s) 2459
EcoT14I CCWWGG 3 cut(s) 2005, 2160, 2958
EcoT22I ATGCAT 2 cut(s) 481, 1621
ErhI CCWWGG 3 cut(s) 2005, 2160, 2958
Esp3I CGTCTC 1 cut(s) 1011
FaqI GGGAC 2 cut(s) 1156, 2264
FbaI TGATCA 1 cut(s) 2973
Fnu4HI GCNGC 9 cut(s) 219, 242, 1908, 2064, 2067, 2070, 2493, 2980, 2983
Fsp4HI GCNGC 9 cut(s) 219, 242, 1908, 2064, 2067, 2070, 2493, 2980, 2983
FspBI CTAG 8 cut(s) 72, 273, 609, 1127, 1548, 2517, 2570, 2763
GlaI GCGC 1 cut(s) 2369
GluI GCNGC 9 cut(s) 219, 242, 1908, 2064, 2067, 2070, 2493, 2980, 2983
HaeIII GGCC 5 cut(s) 221, 241, 463, 2001, 2165
HapII CCGG 3 cut(s) 458, 853, 1520
HgaI GACGC 1 cut(s) 2999
HhaI GCGC 1 cut(s) 2370
Hin6I GCGC 1 cut(s) 2368
HinP1I GCGC 1 cut(s) 2368
HindIII AAGCTT 2 cut(s) 1634, 2220
HpaII CCGG 3 cut(s) 458, 853, 1520
HphI GGTGA 6 cut(s) 245, 339, 521, 578, 698, 2291
Hpy166II GTNNAC 9 cut(s) 6, 64, 391, 1069, 1713, 1919, 2030, 2723, 2894
Hpy8I GTNNAC 9 cut(s) 6, 64, 391, 1069, 1713, 1919, 2030, 2723, 2894
Hpy99I CGWCG 1 cut(s) 2028
HpyCH4IV ACGT 4 cut(s) 253, 341, 1897, 2719
HpyF10VI GCNNNNNNNGC 7 cut(s) 282, 1616, 1687, 1916, 2069, 2171, 3004
HpySE526I ACGT 4 cut(s) 253, 341, 1897, 2719
HspAI GCGC 1 cut(s) 2368
Ksp22I TGATCA 1 cut(s) 2973
Kzo9I GATC 4 cut(s) 1180, 1434, 1993, 2973
LguI GCTCTTC 3 cut(s) 1020, 1419, 2937
LmnI GCTCC 4 cut(s) 282, 2264, 2296, 2707
Lsp1109I GCAGC 7 cut(s) 1894, 2050, 2053, 2081, 2479, 2991, 2994
MabI ACCWGGT 2 cut(s) 291, 2242
MaeI CTAG 8 cut(s) 72, 273, 609, 1127, 1548, 2517, 2570, 2763
MaeII ACGT 4 cut(s) 253, 341, 1897, 2719
MaeIII GTNAC 9 cut(s) 50, 509, 686, 1350, 1422, 1819, 2238, 2386, 2576
MalI GATC 4 cut(s) 1182, 1436, 1995, 2975
MboI GATC 4 cut(s) 1180, 1434, 1993, 2973
MfeI CAATTG 2 cut(s) 1392, 1773
MhlI GDGCHC 6 cut(s) 66, 1071, 1414, 1921, 2261, 2704
MlyI GAGTC 7 cut(s) 161, 275, 512, 1190, 1340, 1430, 2858
MmeI TCCRAC 1 cut(s) 2798
Mph1103I ATGCAT 2 cut(s) 481, 1621
MroXI GAANNNNTTC 4 cut(s) 821, 1533, 2221, 2418
MslI CAYNNNNRTG 4 cut(s) 59, 227, 2025, 2328
MspCI CTTAAG 1 cut(s) 119
MspI CCGG 3 cut(s) 458, 853, 1520
MunI CAATTG 2 cut(s) 1392, 1773
Mva1269I GAATGC 1 cut(s) 2796
MvnI CGCG 1 cut(s) 1506
MwoI GCNNNNNNNGC 7 cut(s) 282, 1616, 1687, 1916, 2069, 2171, 3004
NciI CCSGG 1 cut(s) 459
NdeII GATC 4 cut(s) 1180, 1434, 1993, 2973
NheI GCTAGC 1 cut(s) 272
NlaIV GGNNCC 4 cut(s) 462, 1560, 2002, 2612
NmeAIII GCCGAG 1 cut(s) 667
NmuCI GTSAC 4 cut(s) 509, 686, 1350, 1422
NsiI ATGCAT 2 cut(s) 481, 1621
NspI RCATGY 3 cut(s) 58, 2817, 2828
NspV TTCGAA 1 cut(s) 1051
OliI CACNNNNGTG 1 cut(s) 2025
PaeI GCATGC 1 cut(s) 2828
PaeR7I CTCGAG 1 cut(s) 1641
PagI TCATGA 3 cut(s) 1327, 1417, 2469
PaqCI CACCTGC 1 cut(s) 361
PasI CCCWGGG 1 cut(s) 278
PciI ACATGT 2 cut(s) 54, 2813
PciSI GCTCTTC 3 cut(s) 1020, 1419, 2937
PctI GAATGC 1 cut(s) 2796
PdmI GAANNNNTTC 4 cut(s) 821, 1533, 2221, 2418
PfeI GAWTC 6 cut(s) 534, 596, 755, 2120, 2741, 2805
PfoI TCCNGGA 2 cut(s) 1719, 2089
PkrI GCNGC 9 cut(s) 220, 243, 1909, 2065, 2068, 2071, 2494, 2981, 2984
PleI GAGTC 7 cut(s) 161, 274, 512, 1190, 1339, 1429, 2858
PmaCI CACGTG 1 cut(s) 2720
PmlI CACGTG 1 cut(s) 2720
PpsI GAGTC 7 cut(s) 161, 274, 512, 1190, 1339, 1429, 2858
Ppu21I YACGTR 1 cut(s) 2720
PscI ACATGT 2 cut(s) 54, 2813
PspCI CACGTG 1 cut(s) 2720
PspN4I GGNNCC 4 cut(s) 462, 1560, 2002, 2612
PspPI GGNCC 3 cut(s) 461, 850, 2000
PstI CTGCAG 2 cut(s) 2071, 2589
PstNI CAGNNNCTG 2 cut(s) 1145, 1967
RsaI GTAC 8 cut(s) 5, 252, 1090, 1114, 2031, 2038, 2501, 2893
RsaNI GTAC 8 cut(s) 4, 251, 1089, 1113, 2030, 2037, 2500, 2892
RseI CAYNNNNRTG 4 cut(s) 59, 227, 2025, 2328
SapI GCTCTTC 3 cut(s) 1020, 1419, 2937
SatI GCNGC 9 cut(s) 219, 242, 1908, 2064, 2067, 2070, 2493, 2980, 2983
Sau3AI GATC 4 cut(s) 1180, 1434, 1993, 2973
Sau96I GGNCC 3 cut(s) 461, 850, 2000
ScaI AGTACT 1 cut(s) 1090
SchI GAGTC 7 cut(s) 161, 275, 512, 1190, 1340, 1430, 2858
SduI GDGCHC 6 cut(s) 66, 1071, 1414, 1921, 2261, 2704
SexAI ACCWGGT 2 cut(s) 291, 2242
SfcI CTRYAG 4 cut(s) 1296, 2046, 2067, 2585
Sfr274I CTCGAG 1 cut(s) 1641
SfuI TTCGAA 1 cut(s) 1051
SinI GGWCC 1 cut(s) 850
SlaI CTCGAG 1 cut(s) 1641
SmiMI CAYNNNNRTG 4 cut(s) 59, 227, 2025, 2328
SmlI CTYRAG 4 cut(s) 119, 1641, 1767, 2729
SmoI CTYRAG 4 cut(s) 119, 1641, 1767, 2729
SphI GCATGC 1 cut(s) 2828
SsiI CCGC 4 cut(s) 218, 242, 1688, 2011
SspI AATATT 2 cut(s) 309, 3042
SspMI CTAG 8 cut(s) 72, 273, 609, 1127, 1548, 2517, 2570, 2763
StyI CCWWGG 3 cut(s) 2005, 2160, 2958
TaiI ACGT 4 cut(s) 256, 344, 1900, 2722
TaqI TCGA 5 cut(s) 671, 996, 1051, 1642, 1754
TatI WGTACW 3 cut(s) 3, 1088, 2036
TauI GCSGC 2 cut(s) 221, 244
TfiI GAWTC 6 cut(s) 534, 596, 755, 2120, 2741, 2805
TscAI CASTG 3 cut(s) 66, 789, 2046
TseFI GTSAC 4 cut(s) 509, 686, 1350, 1422
TseI GCWGC 7 cut(s) 1907, 2063, 2066, 2069, 2492, 2979, 2982
Tsp45I GTSAC 4 cut(s) 509, 686, 1350, 1422
TspGWI ACGGA 4 cut(s) 1052, 1683, 2251, 2929
TspRI CASTG 3 cut(s) 66, 789, 2046
Vha464I CTTAAG 1 cut(s) 119
VneI GTGCAC 3 cut(s) 62, 1067, 1917
VpaK11BI GGWCC 1 cut(s) 850
XagI CCTNNNNNAGG 1 cut(s) 2103
XbaI TCTAGA 1 cut(s) 608
XceI RCATGY 3 cut(s) 58, 2817, 2828
XhoI CTCGAG 1 cut(s) 1641
XmnI GAANNNNTTC 4 cut(s) 821, 1533, 2221, 2418
XspI CTAG 8 cut(s) 72, 273, 609, 1127, 1548, 2517, 2570, 2763
ZrmI AGTACT 1 cut(s) 1090
Zsp2I ATGCAT 2 cut(s) 481, 1621
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.