pycom17g27510

Nodal modulator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0011945
Physical Location & Seq
Forward (+)
25647578 .. 25659073
11496 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3381 bp
ATGGTTACTACTTCATTCCCGTATATGACAAGGCAGGGTTCGTTTGTAATTAAAATCAATGGCCCAGAGGGATGGTCATGGAACCCAGACAAGGTTCCTGTTGTTGTTGACGATTCTGGCTGCAATGGCAGTGAAGATATTAACTTTCGGTTTACTGGGTTCTCCTTGTCTGGTAGAGTAGTGGGAGCCGTTGGCGGATGGAGCTGCTCCGTTCAAAATGGAGGTCCTCCAAATATTGAAGTTGAACTTTTGTCTGATACTGGTGATGTTGTTTCCTCTGTTATAACATCAGCAGGGGGTGTTTACATGTTCAAAAATATTATTCCAGGAAAATATGAATTACGTGCTTCACACCCTGACTTGAAAGTTGAAATAAGAGGTTCAACAAAGGTGAATTTGGGTTTCGGAAATGACGTAGTGGATGACATTTTCTTTGTCCCTGGGTATGATGTTCGTGGATTTGTTGTTTCTCAGGGAAATCCAATATTGGGAGTTCACGTTTATTTACACTCAGATGACGTCTTAGAGGTAGACTGTCCCCAAGGTTCTGGAACTGCCTCTGGAACGAGGAAGGCACTCTGCCATGCTGTATCCGATGATCATGGGATGTTCATTTTTAAATCAGTACCTTGTGGAACATATGAGCTCATACCTTACTACAAGGGTGAAAACACAGTATTCGATGTTTCACCTCCTGTCATGTCAGTAACTGTTGAGCATCAACACGTGACAGTTCCTCAGAAGTTCCAGGTCACTGGATTTTCTGTTGGGGGCCGAGTTGTGGATGGAAATGATGAAGGCGTTGAAGGTGTTAAAATTATAGTTGATGGCCATGAAAGGTCCATCACTGACAAACAAGGGTATTACAAACTTGACCAGGTTACTTCCAATCGCTATGCAATTGAAGCCACAAAGGAGCATTACAAGTTCAGCAGTTTGAACGATTATTTGGTATTACCGAATATGGCATCAATTATGGATATTAAAGCAGTTTCCTATGATGTCTGTGGTGTGGTTCATATGGTTACTGCTGGTTACAAGGCAAAGGTAGCTTTGACTCATGGCCCTGAAAATGTCAAACCTCAAGTGAAACAGACTGATGGAAGTGGAAATTTCTGCTTTGAGGTTACACCTGGTGAATATCGTTTATCTGCTTTAGCAGCTACGCCTGAGAGTGCTTCTGGTCTAATGTTTTTGCCATCTTATGTTGATGTTGCTGTAAAAAATCCATTATTGAACGTCAAGTTTTCTCAGGCATTGGTCAATGTTCGTGGAACTGTAACCTGCAAGGAGAAGTGTGGTGCATCTGTTTCTGTTACCCTGGTTGGTTTAGCTGGTAAACGTAATGAAGAGAGGAGGACAGTTAGTTTGACTAACGAGAGTAGTGAATTTCACTTCGAAAGTGTCATCCCAGGGAAATACAGATTTGAGGTCAAACATAATTCAGAAGAACCCACAGCTGTTGAAGATAATTGGTGCTGGGAGAAAAGTTCCATTGATGTGGATGTTGGTGTGGATGATGTAGAAGGAATTGAATTTGTTCAAAAAGGCTACTGGGTCAATGTAATTTCCACCCATGATGTAGATGCTTATATGACTCAGCCAGATGGTTCTTCCATTAATTTGAAAATTAAGAAAGGTTCCCAGAATATTTGTATAGAATATCCTGGAGTGCATGAACTTTATTTTGTCAACTCGTGTATTTTCTTCGGGAGCTCATCAATTGAGATTGATACTTTAAACCCATTGCCTATTTATCTGAAAGGAGAAAAGTATCTTGTTAAAGGACAAATCAATGTTTCATCAAGCTCATTTGAGGGTGTCAGCGAGGTACCTGAGAATTTCATTGTGGATATTCTGAATGCGGGGGGTAGTATTATAGATGAGACCACGGCTTGGCTGTCTTCCAGTGGAAATGATCAAAGTGCCGTAGTGTATGAATATTCTGCATGGGCCAATCGTGGAGAAAGACTTACCTTTGTTCCTCGGGATCCAAGAACTGATGAGACGAGGAAGATCTTGTTTTATCCTAGACAACATCATGTCTTAATTACAAATGATGGCTGTCAAGCTTCAATCCCTCCATTCTCTGGTAGATTGGGTTTATATATTAAAGATCGATCAGTCTCTCCCCCTCTTTCTGAAGTTCATATTAAGATTCTTGCCTCTGGGGATAGCCAGATTGCTCAACTTAAGGATGGTGAACTAGTACTTGAAACTACTACGGGAATGGATGGCTCTTTTGTTGGGGGACCTTTATATGATGAAATAACATACCGTGTTGAGGCTTCAAAGCCTGGGTACCATTTAAAACAAGTTGGGCCTCATTCCTTTTCTTGTCAGAAGCTTGGCCAAATTTCTGTAAATATTCATTCTAAAGATGATGCTAAAGAGCCTATTCCTTCAGTGTTATTATCTTTGAGTGGTGATGATGGCTACAGAAACAACTCAGTATCTGCAGCTGGTGGAGCATTCCTGTTCAACAACTTATTTCCAGGGACATTCTATCTCCGTCCTTTGCTAAAGGAGTTTGCCTTTTCTCCTCCGGCACTGGCGATAGATCTTGGTTCTGGGGAGTCTAAAGAAGCTATTTTCCTGGCTACTCGAGTGGCTTACAGTGCTATGGGTGTCGTTACTCTAGTGTCTGGCCAACCAAAAGAAGGTGTGTTAGTTGAGGCCAGATCAGAATCGAAAGGCTATTATGAGGAAACAGTGACTGATTCATCCGGAAGTTATCGTTTGAGAGGACTTCTTCCTGACGCAATCTATGTAATCAAAGTAGTGAAAAGGGATGGTTTGGGTAGCGCTAAAATTGAGCGTGCATCACCAGAATATGTTCCTATCACGGTTGGGCATGAGGATATCAAAGGATTAGATTTTCTGGTTTTTGAACAACCAGACACAACTATTTTAAGTTGCCATGTTGAAGGAAAGAGAATAAAGGAACTGCATCCACATCTACTAGTGGAAATCAAATCTAGTGATCTATCTATAACTGAGTCCGTCTTCCCTCTTCCGCTTTCCAACTTTTTCCAGGTGAAGGACTTACCCAAGGGTAAGCACCTTCTGCAGCTTCGTTATAGTCTACCATCAAGCAGCCATAAGTTTAAATCCGAGGTTATCGAGGTTGATTTAGAGCAGAATACTCGCATGCATGTTGGCCCACTCAGATATGCATTTGAAGAGGATCAGCAGAAACAGGAGTTGACTCCGGCACCAGTTTTCCCTCTTATAGTCGGTGTGTCAGTGATTGCTCTGTTTGCCACTATTCCAAGGTTGAAGGACTTGTACCAATCCACGGTGGGAATACCAACACCTGGATTCACCACAACTGCAAAGAAGGAAGTACGAAAGCCTGTTTTGAGAAAGAAGACTTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CarboxypepD_reg

Protein Analysis

1127

Amino Acids

123.25

Weight (kDa)

5.54

Isoelectric Point (pI)

33.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NOMO1-like_1st PF22898 11 - 49 1.4e-09 BOS complex subunit NOMO1-like, first beta sandwich domain
NOMO1-like_2nd PF22904 51 - 143 7.8e-16 BOS complex subunit NOMO1-like, second beta sandwich domain
NOMO_3rd PF23193 190 - 252 5e-09 NOMO third transthyretin-like domain
NOMO1-like_2nd PF22904 252 - 312 4.3e-07 BOS complex subunit NOMO1-like, second beta sandwich domain
CarboxypepD_reg PF13620 255 - 314 2.5e-06 Carboxypeptidase regulatory-like domain
NOMO_5th PF23194 334 - 416 1.6e-10 NOMO fifth transthyretin-like domain
NOMO_6th PF23196 421 - 498 7.5e-07 NOMO sixth transthyretin-like domain
Ig_NOMO PF23141 519 - 591 4.6e-25 NOMO Ig-like domain
NOMO_8th PF23660 593 - 699 8e-40 NOMO eighth prealbumin-like domain
NOMO1-like_9th PF22902 708 - 774 2.4e-09 BOS complex subunit NOMO1-like, beta sandwich domain
CarboxypepD_reg PF13620 876 - 935 2e-06 Carboxypeptidase regulatory-like domain
DUF7152 PF23662 968 - 1065 1.3e-43 Domain of unknown function (DUF7152)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0011945)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G62360 AT3G62360
fragaria_vesca FvH4_1g27060
malus_domestica MD09G1284300.v1.1 MD17G1277500.v1.1
prunus_persica Prupe.3G010500_v2.0.a1 Prupe.3G010500_v2.0.a1 Prupe.3G010500_v2.0.a1
pyrus_communis pycom17g27510
rosa_chinensis RchiOBHm_Chr3g0487381
rosa_laevigata RLG00000023009
rosa_multiflora Rmu_sc0001955.1_g000004
rosa_roxburghii Rroxscaffold_6G00394840
rosa_rugosa Rorug03G0233600
rosa_samantha Rh3AG281600 Rh3BG317200 Rh3CG315300 Rh3DG313200
rosa_wichuraiana Rw3G024930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 284
AatII GACGTC 1 cut(s) 522
Acc36I ACCTGC 1 cut(s) 1292
Acc65I GGTACC 2 cut(s) 1831, 2301
AccB1I GGYRCC 3 cut(s) 1831, 2301, 3214
AccB7I CCANNNNNTGG 3 cut(s) 1896, 2090, 3317
AccI GTMKAC 2 cut(s) 531, 3085
AccIII TCCGGA 1 cut(s) 2726
AciI CCGC 3 cut(s) 195, 1865, 3017
AclWI GGATC 3 cut(s) 1985, 1998, 3195
AcoI YGGCCR 3 cut(s) 829, 2350, 2647
AcsI RAATTY 6 cut(s) 394, 1111, 1388, 1535, 1840, 2355
AcuI CTGAAG 2 cut(s) 2163, 2388
AcvI CACGTG 1 cut(s) 727
AcyI GRCGYC 1 cut(s) 519
AdeI CACNNNGTG 1 cut(s) 1136
AfaI GTAC 6 cut(s) 627, 1833, 2211, 2303, 3290, 3348
AfeI AGCGCT 1 cut(s) 2806
AflII CTTAAG 1 cut(s) 2192
AflIII ACRYGT 2 cut(s) 306, 724
AhlI ACTAGT 2 cut(s) 2206, 2962
AjuI GAANNNNNNNTTGG 2 cut(s) 932, 964
Alw21I GWGCWC 2 cut(s) 648, 1718
Alw26I GTCTC 3 cut(s) 1880, 2000, 2131
AlwI GGATC 3 cut(s) 1985, 1998, 3195
AlwNI CAGNNNCTG 3 cut(s) 710, 2456, 2717
Ama87I CYCGRG 2 cut(s) 1986, 2604
Aor13HI TCCGGA 1 cut(s) 2726
Aor51HI AGCGCT 1 cut(s) 2806
ApeKI GCWGC 6 cut(s) 120, 204, 1160, 2459, 3070, 3096
ApoI RAATTY 6 cut(s) 394, 1111, 1388, 1535, 1840, 2355
ArsI GACNNNNNNTTYG 6 cut(s) 208, 240, 416, 448, 1351, 1383
AseI ATTAAT 1 cut(s) 1620
Asp700I GAANNNNTTC 2 cut(s) 1539, 2835
Asp718I GGTACC 2 cut(s) 1831, 2301
AspLEI GCGC 1 cut(s) 2807
AspS9I GGNCC 9 cut(s) 62, 224, 772, 840, 1064, 1953, 2252, 2321, 3161
AsuII TTCGAA 1 cut(s) 1398
AvaI CYCGRG 2 cut(s) 1986, 2604
AvaII GGWCC 3 cut(s) 224, 840, 2252
BalI TGGCCA 3 cut(s) 831, 2352, 2649
BamHI GGATCC 1 cut(s) 1990
BanI GGYRCC 3 cut(s) 1831, 2301, 3214
BanII GRGCYC 2 cut(s) 648, 1718
BarI GAAGNNNNNNTAC 2 cut(s) 3272, 3304
BauI CACGAG 1 cut(s) 1696
BbrPI CACGTG 1 cut(s) 727
BbsI GAAGAC 3 cut(s) 1896, 2998, 3377
Bbv12I GWGCWC 2 cut(s) 648, 1718
BbvI GCAGC 6 cut(s) 107, 191, 1172, 2471, 3082, 3108
BceAI ACGGC 3 cut(s) 173, 1908, 1913
BcgI CGANNNNNNTGC 2 cut(s) 3128, 3162
BciVI GTATCC 1 cut(s) 601
BclI TGATCA 2 cut(s) 598, 1918
BcoDI GTCTC 3 cut(s) 1880, 2000, 2131
BcuI ACTAGT 2 cut(s) 2206, 2962
BfaI CTAG 5 cut(s) 2031, 2207, 2639, 2963, 2979
BfmI CTRYAG 3 cut(s) 2437, 2457, 3068
BfoI RGCGCY 1 cut(s) 2808
BfrI CTTAAG 1 cut(s) 2192
BfuAI ACCTGC 1 cut(s) 1292
BfuI GTATCC 1 cut(s) 601
BglII AGATCT 2 cut(s) 2016, 2560
BisI GCNGC 6 cut(s) 121, 205, 1161, 2460, 3071, 3097
BlsI GCNGC 6 cut(s) 122, 206, 1162, 2461, 3072, 3098
BmcAI AGTACT 1 cut(s) 2211
Bme18I GGWCC 3 cut(s) 224, 840, 2252
BmeT110I CYCGRG 2 cut(s) 1986, 2604
BmgT120I GGNCC 9 cut(s) 62, 224, 772, 840, 1064, 1953, 2252, 2321, 3161
BmrI ACTGGG 2 cut(s) 165, 1564
BmsI GCATC 7 cut(s) 727, 977, 1313, 1576, 2374, 2831, 2959
BmuI ACTGGG 2 cut(s) 165, 1564
BpiI GAAGAC 3 cut(s) 1896, 2998, 3377
BpmI CTGGAG 1 cut(s) 1689
Bpu14I TTCGAA 1 cut(s) 1398
BpuEI CTTGAG 1 cut(s) 1068
Bsa29I ATCGAT 1 cut(s) 2119
BsaAI YACGTR 2 cut(s) 344, 727
BsaBI GATNNNNATC 1 cut(s) 2686
BsaHI GRCGYC 1 cut(s) 519
BsaI GGTCTC 1 cut(s) 1880
BsaWI WCCGGW 1 cut(s) 2726
BsaXI ACNNNNNCTCC 2 cut(s) 483, 513
Bse1I ACTGG 7 cut(s) 160, 265, 760, 1559, 1908, 2556, 3218
Bse3DI GCAATG 2 cut(s) 130, 1745
Bse8I GATNNNNATC 1 cut(s) 2686
BseAI TCCGGA 1 cut(s) 2726
BseCI ATCGAT 1 cut(s) 2119
BseJI GATNNNNATC 1 cut(s) 2686
BseMI GCAATG 2 cut(s) 130, 1745
BseNI ACTGG 7 cut(s) 160, 265, 760, 1559, 1908, 2556, 3218
BseRI GAGGAG 2 cut(s) 1369, 2532
BseXI GCAGC 6 cut(s) 107, 191, 1172, 2471, 3082, 3108
BseYI CCCAGC 1 cut(s) 1479
BshNI GGYRCC 3 cut(s) 1831, 2301, 3214
BshVI ATCGAT 1 cut(s) 2119
BsiHKAI GWGCWC 2 cut(s) 648, 1718
BsiHKCI CYCGRG 2 cut(s) 1986, 2604
BsiSI CCGG 3 cut(s) 2546, 2727, 3212
BslFI GGGAC 4 cut(s) 422, 522, 2265, 2512
BsmAI GTCTC 3 cut(s) 1880, 2000, 2131
BsmBI CGTCTC 1 cut(s) 2000
BsmFI GGGAC 4 cut(s) 422, 522, 2265, 2512
BsmI GAATGC 2 cut(s) 1867, 2471
Bso31I GGTCTC 1 cut(s) 1880
BsoBI CYCGRG 2 cut(s) 1986, 2604
Bsp119I TTCGAA 1 cut(s) 1398
Bsp1286I GDGCHC 2 cut(s) 648, 1718
Bsp13I TCCGGA 1 cut(s) 2726
BspACI CCGC 3 cut(s) 195, 1865, 3017
BspDI ATCGAT 1 cut(s) 2119
BspEI TCCGGA 1 cut(s) 2726
BspMAI CTGCAG 2 cut(s) 2461, 3072
BspMI ACCTGC 1 cut(s) 1292
BspPI GGATC 3 cut(s) 1985, 1998, 3195
BspT104I TTCGAA 1 cut(s) 1398
BspT107I GGYRCC 3 cut(s) 1831, 2301, 3214
BspTI CTTAAG 1 cut(s) 2192
BspTNI GGTCTC 1 cut(s) 1880
BsrDI GCAATG 2 cut(s) 130, 1745
BsrI ACTGG 7 cut(s) 160, 265, 760, 1559, 1908, 2556, 3218
BssNI GRCGYC 1 cut(s) 519
BssSI CACGAG 1 cut(s) 1696
BssT1I CCWWGG 3 cut(s) 541, 3051, 3272
Bst2BI CACGAG 1 cut(s) 1696
Bst6I CTCTTC 3 cut(s) 1344, 3018, 3177
BstACI GRCGYC 1 cut(s) 519
BstAFI CTTAAG 1 cut(s) 2192
BstAPI GCANNNNNTGC 1 cut(s) 3067
BstBAI YACGTR 2 cut(s) 344, 727
BstBI TTCGAA 1 cut(s) 1398
BstC8I GCNNGC 2 cut(s) 2820, 3152
BstDSI CCRYGG 2 cut(s) 1890, 3297
BstH2I RGCGCY 1 cut(s) 2808
BstHHI GCGC 1 cut(s) 2807
BstMAI GTCTC 3 cut(s) 1880, 2000, 2131
BstMWI GCNNNNNNNGC 9 cut(s) 126, 201, 905, 1049, 1160, 2468, 2618, 3067, 3260
BstNSI RCATGY 3 cut(s) 310, 3154, 3158
BstSFI CTRYAG 3 cut(s) 2437, 2457, 3068
BstV1I GCAGC 6 cut(s) 107, 191, 1172, 2471, 3082, 3108
BstV2I GAAGAC 3 cut(s) 1896, 2998, 3377
BstX2I RGATCY 3 cut(s) 1990, 2016, 2560
BstXI CCANNNNNNTGG 4 cut(s) 72, 548, 755, 1501
BstYI RGATCY 3 cut(s) 1990, 2016, 2560
Bsu15I ATCGAT 1 cut(s) 2119
BsuI GTATCC 1 cut(s) 601
BsuTUI ATCGAT 1 cut(s) 2119
BtgI CCRYGG 2 cut(s) 1890, 3297
BtsI GCAGTG 1 cut(s) 136
BtsIMutI CAGTG 9 cut(s) 136, 753, 846, 1915, 2412, 2549, 2623, 2718, 3252
BveI ACCTGC 1 cut(s) 1292
Cac8I GCNNGC 2 cut(s) 2820, 3152
CaiI CAGNNNCTG 3 cut(s) 710, 2456, 2717
CfoI GCGC 1 cut(s) 2807
Cfr13I GGNCC 9 cut(s) 62, 224, 772, 840, 1064, 1953, 2252, 2321, 3161
ClaI ATCGAT 1 cut(s) 2119
CseI GACGC 1 cut(s) 2768
CsiI ACCWGGT 2 cut(s) 876, 1132
Csp6I GTAC 6 cut(s) 626, 1832, 2210, 2302, 3289, 3347
CspCI CAANNNNNGTGG 4 cut(s) 1252, 1287, 1444, 1479
CviQI GTAC 6 cut(s) 626, 1832, 2210, 2302, 3289, 3347
DraI TTTAAA 4 cut(s) 619, 1740, 2310, 3109
DraIII CACNNNGTG 1 cut(s) 1136
EaeI YGGCCR 3 cut(s) 829, 2350, 2647
Eam1104I CTCTTC 3 cut(s) 1344, 3018, 3177
EarI CTCTTC 3 cut(s) 1344, 3018, 3177
EciI GGCGGA 1 cut(s) 210
Ecl136II GAGCTC 2 cut(s) 646, 1716
Eco130I CCWWGG 3 cut(s) 541, 3051, 3272
Eco24I GRGCYC 2 cut(s) 648, 1718
Eco31I GGTCTC 1 cut(s) 1880
Eco32I GATATC 1 cut(s) 2863
Eco47I GGWCC 3 cut(s) 224, 840, 2252
Eco47III AGCGCT 1 cut(s) 2806
Eco53kI GAGCTC 2 cut(s) 646, 1716
Eco57I CTGAAG 2 cut(s) 2163, 2388
Eco72I CACGTG 1 cut(s) 727
Eco88I CYCGRG 2 cut(s) 1986, 2604
EcoICRI GAGCTC 2 cut(s) 646, 1716
EcoO109I RGGNCCY 2 cut(s) 224, 2252
EcoRV GATATC 1 cut(s) 2863
EcoT14I CCWWGG 3 cut(s) 541, 3051, 3272
EcoT22I ATGCAT 2 cut(s) 3156, 3178
EcoT38I GRGCYC 2 cut(s) 648, 1718
ErhI CCWWGG 3 cut(s) 541, 3051, 3272
Esp3I CGTCTC 1 cut(s) 2000
FalI AAGNNNNNCTT 2 cut(s) 231, 263
FaqI GGGAC 4 cut(s) 422, 522, 2265, 2512
FauI CCCGC 1 cut(s) 1858
FauNDI CATATG 2 cut(s) 640, 1020
FbaI TGATCA 2 cut(s) 598, 1918
FblI GTMKAC 2 cut(s) 531, 3085
Fnu4HI GCNGC 6 cut(s) 121, 205, 1161, 2460, 3071, 3097
FriOI GRGCYC 2 cut(s) 648, 1718
Fsp4HI GCNGC 6 cut(s) 121, 205, 1161, 2460, 3071, 3097
FspBI CTAG 5 cut(s) 2031, 2207, 2639, 2963, 2979
GlaI GCGC 1 cut(s) 2806
GluI GCNGC 6 cut(s) 121, 205, 1161, 2460, 3071, 3097
GsaI CCCAGC 1 cut(s) 1483
GsuI CTGGAG 1 cut(s) 1689
HaeII RGCGCY 1 cut(s) 2808
HapII CCGG 3 cut(s) 2546, 2727, 3212
HgaI GACGC 1 cut(s) 2768
HhaI GCGC 1 cut(s) 2807
Hin1I GRCGYC 1 cut(s) 519
Hin6I GCGC 1 cut(s) 2805
HinP1I GCGC 1 cut(s) 2805
HincII GTYRAC 3 cut(s) 109, 1693, 3207
HindII GTYRAC 3 cut(s) 109, 1693, 3207
HindIII AAGCTT 2 cut(s) 2070, 2345
HpaII CCGG 3 cut(s) 2546, 2727, 3212
Hpy188III TCNNGA 6 cut(s) 549, 561, 1711, 1988, 2727, 2756
HpyCH4IV ACGT 7 cut(s) 343, 414, 498, 519, 726, 1239, 1342
HpyF10VI GCNNNNNNNGC 9 cut(s) 126, 201, 905, 1049, 1160, 2468, 2618, 3067, 3260
HpySE526I ACGT 7 cut(s) 343, 414, 498, 519, 726, 1239, 1342
Hsp92I GRCGYC 1 cut(s) 519
HspAI GCGC 1 cut(s) 2805
Kpn2I TCCGGA 1 cut(s) 2726
KpnI GGTACC 2 cut(s) 1835, 2305
Ksp22I TGATCA 2 cut(s) 598, 1918
LmnI GCTCC 6 cut(s) 185, 201, 212, 916, 1713, 2468
Lsp1109I GCAGC 6 cut(s) 107, 191, 1172, 2471, 3082, 3108
LweI GCATC 7 cut(s) 727, 977, 1313, 1576, 2374, 2831, 2959
MabI ACCWGGT 2 cut(s) 876, 1132
MaeI CTAG 5 cut(s) 2031, 2207, 2639, 2963, 2979
MaeII ACGT 7 cut(s) 343, 414, 498, 519, 726, 1239, 1342
MfeI CAATTG 2 cut(s) 900, 1722
MflI RGATCY 3 cut(s) 1990, 2016, 2560
MhlI GDGCHC 2 cut(s) 648, 1718
MlsI TGGCCA 3 cut(s) 831, 2352, 2649
MluNI TGGCCA 3 cut(s) 831, 2352, 2649
MlyI GAGTC 5 cut(s) 1051, 1591, 2585, 3008, 3202
MmeI TCCRAC 1 cut(s) 3048
Mox20I TGGCCA 3 cut(s) 831, 2352, 2649
Mph1103I ATGCAT 2 cut(s) 3156, 3178
MroI TCCGGA 1 cut(s) 2726
MroXI GAANNNNTTC 2 cut(s) 1539, 2835
MscI TGGCCA 3 cut(s) 831, 2352, 2649
MslI CAYNNNNRTG 2 cut(s) 513, 1499
Msp20I TGGCCA 3 cut(s) 831, 2352, 2649
MspA1I CMGCKG 2 cut(s) 1460, 2462
MspCI CTTAAG 1 cut(s) 2192
MspI CCGG 3 cut(s) 2546, 2727, 3212
MunI CAATTG 2 cut(s) 900, 1722
Mva1269I GAATGC 2 cut(s) 1867, 2471
MwoI GCNNNNNNNGC 9 cut(s) 126, 201, 905, 1049, 1160, 2468, 2618, 3067, 3260
NdeI CATATG 2 cut(s) 640, 1020
NmeAIII GCCGAG 1 cut(s) 800
NmuCI GTSAC 3 cut(s) 727, 751, 2713
NsiI ATGCAT 2 cut(s) 3156, 3178
NspI RCATGY 3 cut(s) 310, 3154, 3158
NspV TTCGAA 1 cut(s) 1398
PaeI GCATGC 1 cut(s) 3154
PaeR7I CTCGAG 1 cut(s) 2604
PasI CCCWGGG 2 cut(s) 440, 1412
PciI ACATGT 1 cut(s) 306
PcsI WCGNNNNNNNCGW 1 cut(s) 411
PctI GAATGC 2 cut(s) 1867, 2471
PdmI GAANNNNTTC 2 cut(s) 1539, 2835
PfeI GAWTC 5 cut(s) 113, 2158, 2687, 2720, 3321
PflMI CCANNNNNTGG 3 cut(s) 1896, 2090, 3317
PfoI TCCNGGA 2 cut(s) 325, 1666
PkrI GCNGC 6 cut(s) 122, 206, 1162, 2461, 3072, 3098
PleI GAGTC 5 cut(s) 1051, 1591, 2584, 3007, 3202
PmaCI CACGTG 1 cut(s) 727
PmlI CACGTG 1 cut(s) 727
PpsI GAGTC 5 cut(s) 1051, 1591, 2584, 3007, 3202
Ppu21I YACGTR 2 cut(s) 344, 727
PpuMI RGGWCCY 2 cut(s) 224, 2252
PscI ACATGT 1 cut(s) 306
PshBI ATTAAT 1 cut(s) 1620
PsiI TTATAA 1 cut(s) 284
Psp124BI GAGCTC 2 cut(s) 648, 1718
Psp5II RGGWCCY 2 cut(s) 224, 2252
PspCI CACGTG 1 cut(s) 727
PspFI CCCAGC 1 cut(s) 1479
PspPI GGNCC 9 cut(s) 62, 224, 772, 840, 1064, 1953, 2252, 2321, 3161
PspPPI RGGWCCY 2 cut(s) 224, 2252
PspXI VCTCGAGB 1 cut(s) 2604
PstI CTGCAG 2 cut(s) 2461, 3072
PstNI CAGNNNCTG 3 cut(s) 710, 2456, 2717
PsuI RGATCY 3 cut(s) 1990, 2016, 2560
PvuII CAGCTG 2 cut(s) 1460, 2462
RsaI GTAC 6 cut(s) 627, 1833, 2211, 2303, 3290, 3348
RsaNI GTAC 6 cut(s) 626, 1832, 2210, 2302, 3289, 3347
RseI CAYNNNNRTG 2 cut(s) 513, 1499
SacI GAGCTC 2 cut(s) 648, 1718
SatI GCNGC 6 cut(s) 121, 205, 1161, 2460, 3071, 3097
Sau96I GGNCC 9 cut(s) 62, 224, 772, 840, 1064, 1953, 2252, 2321, 3161
ScaI AGTACT 1 cut(s) 2211
SchI GAGTC 5 cut(s) 1051, 1591, 2585, 3008, 3202
SduI GDGCHC 2 cut(s) 648, 1718
SexAI ACCWGGT 2 cut(s) 876, 1132
SfaNI GCATC 7 cut(s) 727, 977, 1313, 1576, 2374, 2831, 2959
SfcI CTRYAG 3 cut(s) 2437, 2457, 3068
Sfr274I CTCGAG 1 cut(s) 2604
SfuI TTCGAA 1 cut(s) 1398
SinI GGWCC 3 cut(s) 224, 840, 2252
SlaI CTCGAG 1 cut(s) 2604
SmiMI CAYNNNNRTG 2 cut(s) 513, 1499
SmlI CTYRAG 3 cut(s) 1083, 2192, 2604
SmoI CTYRAG 3 cut(s) 1083, 2192, 2604
SpeI ACTAGT 2 cut(s) 2206, 2962
SphI GCATGC 1 cut(s) 3154
SsiI CCGC 3 cut(s) 195, 1865, 3017
SspI AATATT 6 cut(s) 235, 319, 486, 1651, 1943, 2368
SspMI CTAG 5 cut(s) 2031, 2207, 2639, 2963, 2979
SstI GAGCTC 2 cut(s) 648, 1718
StyI CCWWGG 3 cut(s) 541, 3051, 3272
TaiI ACGT 7 cut(s) 346, 417, 501, 522, 729, 1242, 1345
TaqI TCGA 6 cut(s) 681, 1398, 2119, 2605, 2690, 3123
TatI WGTACW 1 cut(s) 2209
TfiI GAWTC 5 cut(s) 113, 2158, 2687, 2720, 3321
TscAI CASTG 9 cut(s) 136, 760, 853, 1915, 2412, 2556, 2623, 2718, 3252
TseFI GTSAC 3 cut(s) 727, 751, 2713
TseI GCWGC 6 cut(s) 120, 204, 1160, 2459, 3070, 3096
Tsp45I GTSAC 3 cut(s) 727, 751, 2713
TspGWI ACGGA 3 cut(s) 199, 2501, 2992
TspRI CASTG 9 cut(s) 136, 760, 853, 1915, 2412, 2556, 2623, 2718, 3252
Van91I CCANNNNNTGG 3 cut(s) 1896, 2090, 3317
Vha464I CTTAAG 1 cut(s) 2192
VpaK11BI GGWCC 3 cut(s) 224, 840, 2252
VspI ATTAAT 1 cut(s) 1620
XapI RAATTY 6 cut(s) 394, 1111, 1388, 1535, 1840, 2355
XceI RCATGY 3 cut(s) 310, 3154, 3158
XhoI CTCGAG 1 cut(s) 2604
XmiI GTMKAC 2 cut(s) 531, 3085
XmnI GAANNNNTTC 2 cut(s) 1539, 2835
XspI CTAG 5 cut(s) 2031, 2207, 2639, 2963, 2979
ZraI GACGTC 1 cut(s) 520
ZrmI AGTACT 1 cut(s) 2211
Zsp2I ATGCAT 2 cut(s) 3156, 3178
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.