pycom17g28100

Glycosyl hydrolases family 28

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0007246
Physical Location & Seq
Forward (+)
26141368 .. 26143662
2295 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1239 bp
ATGGAGAACAAAAGAAAAAGCAGCAGCTACAATCATGGTGTGGTATGGTATCCAAGGACCAACGTCATGTGCACGTTTGTTCAGGTGGTGAATATGCTTCTGCTACTTGGATTGCTCGGCAATGCTGCTCCGTGGTCTGTCAAGGGCAGCCCATACTGCAGCAATCAGACCAACTCAGGAGCAATTCGACCTCACAGTGTCGCCATCGCTGAATTTGGCGCCGTAGGAGATGGGAACTCCGATGATTGGCCAATTGTTGAACCATTACCATCATACGGTCGAGGAAGGGAGTTGACAGGAGGAAGACATCGAAGTCTAATCTATGGACGCAATTTGACGGACATTATCATAACTGGTGATAATAGATCCAGTGGTGACCAAGGTAGCATCTGGTGGGATTGGTTCCATAATAAAACCTTGAATTATACAAGGCCCCATTTGTTGGAGTTGATAAACTCAACTGGGGTTGTCATTTCAAACCTAACCTTCCTAAGTTCACCATTTTGGACCCTTCACCTGTATATTGCAGGTTACCATGTTACCATCCATAATCGGACAATCCTTGCTCCTCTCAAGTCTCCAAACACGACGGCATTGATCCAGCCAGACTCTTCAGATAATCTCTGCATTGAAGACTGTCATATTAGTACTGGTGATGATCTGGTTTCCATCAAAAATGGGTGGGATGAGTATGGCACTTCATATGGTCGTCCCAGTAGAAACATCATCCTTGTAAGAATTACTGGTAAAACTGAAACAAGCGCAGGAATTGCAATTGGAAGTGAGATGTTGGGAGGTGTGTCAGAAGTTCATGCAGAAGACCTCCACTTTTTCGGTTCCAAAACAGAAATATATATGTATCAAAATTCAAACATGACCTTGGATGGTATAGGAATAGCTATAAGGTTCACCAGTCAGTATGAGGAGCGCCCAGATGAGTTTTACGACCCAAATGCCCTCATTGTAGAGCGAATTACTTTAAAAGATATCATAGTAGAGTGTAGAGAATATCAAAACTGCTGGCCTCCTAGAGGTTTAGAAGGTGAAAATTTTCTCATCATTTGCTTATCCAATATAACCCGTAGTTCTGACTCAGTTTCTCCGGAAACTTGTACGCCTCTCAAGCAGAGAATCTCCCCAGAGCATTACTCGGTTTGTTACTCTCTATCTGATCATTCTGATGACATTTGGATTTCTTCAAGTAATCGAAACAGAGGTGCTTGGCGGCTTTCTTGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

413

Amino Acids

46.16

Weight (kDa)

5.71

Isoelectric Point (pI)

44.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 104 - 282 1.5e-14 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 312
Acc36I ACCTGC 1 cut(s) 518
AccB1I GGYRCC 1 cut(s) 218
AccB7I CCANNNNNTGG 1 cut(s) 442
AccIII TCCGGA 1 cut(s) 1102
AciI CCGC 1 cut(s) 1225
AclWI GGATC 2 cut(s) 360, 592
AcoI YGGCCR 1 cut(s) 248
AcsI RAATTY 3 cut(s) 212, 865, 1048
AcuI CTGAAG 1 cut(s) 597
AcyI GRCGYC 1 cut(s) 219
AfaI GTAC 2 cut(s) 649, 1114
AfiI CCNNNNNNNGG 4 cut(s) 246, 275, 442, 1031
AgsI TTSAA 6 cut(s) 260, 421, 477, 632, 870, 1200
AluBI AGCT 2 cut(s) 27, 899
AluI AGCT 2 cut(s) 27, 899
Alw21I GWGCWC 1 cut(s) 74
Alw26I GTCTC 1 cut(s) 582
Alw44I GTGCAC 1 cut(s) 70
AlwI GGATC 2 cut(s) 360, 592
Aor13HI TCCGGA 1 cut(s) 1102
AoxI GGCC 3 cut(s) 248, 431, 1022
ApaLI GTGCAC 1 cut(s) 70
ApeKI GCWGC 5 cut(s) 21, 24, 125, 147, 159
ApoI RAATTY 3 cut(s) 212, 865, 1048
Asp700I GAANNNNTTC 1 cut(s) 1050
AspLEI GCGC 3 cut(s) 221, 764, 930
AspS9I GGNCC 3 cut(s) 57, 432, 507
AsuHPI GGTGA 8 cut(s) 100, 368, 386, 489, 506, 665, 901, 1055
AvaII GGWCC 2 cut(s) 57, 507
BaeGI GKGCMC 1 cut(s) 74
BalI TGGCCA 1 cut(s) 250
BanI GGYRCC 1 cut(s) 218
BbsI GAAGAC 3 cut(s) 310, 639, 825
Bbv12I GWGCWC 1 cut(s) 74
BbvI GCAGC 5 cut(s) 33, 36, 112, 159, 171
BccI CCATC 6 cut(s) 212, 224, 277, 551, 677, 878
BceAI ACGGC 2 cut(s) 206, 606
BcgI CGANNNNNNTGC 2 cut(s) 935, 969
BciVI GTATCC 1 cut(s) 60
BclI TGATCA 1 cut(s) 1171
BcoDI GTCTC 1 cut(s) 582
BfaI CTAG 1 cut(s) 1029
BfmI CTRYAG 1 cut(s) 157
BfoI RGCGCY 2 cut(s) 222, 931
BfuAI ACCTGC 1 cut(s) 518
BfuI GTATCC 1 cut(s) 60
BisI GCNGC 6 cut(s) 22, 25, 126, 148, 160, 1226
BlsI GCNGC 6 cut(s) 23, 26, 127, 149, 161, 1227
BmcAI AGTACT 1 cut(s) 649
Bme18I GGWCC 2 cut(s) 57, 507
BmgT120I GGNCC 3 cut(s) 57, 432, 507
BmiI GGNNCC 5 cut(s) 220, 404, 434, 509, 838
BmrI ACTGGG 2 cut(s) 471, 708
BmsI GCATC 1 cut(s) 396
BmuI ACTGGG 2 cut(s) 471, 708
BoxI GACNNNNGTC 1 cut(s) 62
BpiI GAAGAC 3 cut(s) 310, 639, 825
BplI GAGNNNNNCTC 2 cut(s) 1133, 1165
BpuEI CTTGAG 2 cut(s) 557, 1106
BsaHI GRCGYC 1 cut(s) 219
BsaJI CCNNGG 4 cut(s) 53, 131, 379, 879
BsaWI WCCGGW 1 cut(s) 1102
Bsc4I CCNNNNNNNGG 4 cut(s) 246, 275, 442, 1031
Bse1I ACTGG 7 cut(s) 358, 369, 466, 655, 714, 748, 912
Bse3DI GCAATG 1 cut(s) 127
BseAI TCCGGA 1 cut(s) 1102
BseDI CCNNGG 4 cut(s) 53, 131, 379, 879
BseGI GGATG 4 cut(s) 543, 691, 726, 889
BseLI CCNNNNNNNGG 4 cut(s) 246, 275, 442, 1031
BseMI GCAATG 1 cut(s) 127
BseMII CTCAG 2 cut(s) 189, 1107
BseNI ACTGG 7 cut(s) 358, 369, 466, 655, 714, 748, 912
BseRI GAGGAG 2 cut(s) 558, 938
BseSI GKGCMC 1 cut(s) 74
BseXI GCAGC 5 cut(s) 33, 36, 112, 159, 171
Bsh1285I CGRYCG 1 cut(s) 280
BshFI GGCC 3 cut(s) 250, 433, 1024
BshNI GGYRCC 1 cut(s) 218
BsiEI CGRYCG 1 cut(s) 280
BsiHKAI GWGCWC 1 cut(s) 74
BsiSI CCGG 1 cut(s) 1103
BslFI GGGAC 1 cut(s) 696
BslI CCNNNNNNNGG 4 cut(s) 246, 275, 442, 1031
BsmAI GTCTC 1 cut(s) 582
BsmFI GGGAC 1 cut(s) 696
BsnI GGCC 3 cut(s) 250, 433, 1024
Bsp1286I GDGCHC 1 cut(s) 74
Bsp13I TCCGGA 1 cut(s) 1102
Bsp143I GATC 4 cut(s) 365, 597, 658, 1171
BspACI CCGC 1 cut(s) 1225
BspANI GGCC 3 cut(s) 250, 433, 1024
BspCNI CTCAG 2 cut(s) 188, 1106
BspEI TCCGGA 1 cut(s) 1102
BspLI GGNNCC 5 cut(s) 220, 404, 434, 509, 838
BspMAI CTGCAG 1 cut(s) 161
BspMI ACCTGC 1 cut(s) 518
BspPI GGATC 2 cut(s) 360, 592
BspT107I GGYRCC 1 cut(s) 218
BsrDI GCAATG 1 cut(s) 127
BsrI ACTGG 7 cut(s) 358, 369, 466, 655, 714, 748, 912
BssECI CCNNGG 4 cut(s) 53, 131, 379, 879
BssMI GATC 4 cut(s) 365, 597, 658, 1171
BssNI GRCGYC 1 cut(s) 219
BssT1I CCWWGG 3 cut(s) 53, 379, 879
Bst4CI ACNGT 3 cut(s) 197, 278, 638
Bst6I CTCTTC 1 cut(s) 616
BstACI GRCGYC 1 cut(s) 219
BstAPI GCANNNNNTGC 1 cut(s) 770
BstC8I GCNNGC 1 cut(s) 1022
BstDEI CTNAG 3 cut(s) 175, 491, 1093
BstDSI CCRYGG 1 cut(s) 131
BstEII GGTNACC 2 cut(s) 374, 530
BstENI CCTNNNNNAGG 1 cut(s) 1029
BstF5I GGATG 4 cut(s) 543, 691, 726, 889
BstH2I RGCGCY 2 cut(s) 222, 931
BstHHI GCGC 3 cut(s) 221, 764, 930
BstKTI GATC 4 cut(s) 368, 600, 661, 1174
BstMAI GTCTC 1 cut(s) 582
BstMBI GATC 4 cut(s) 365, 597, 658, 1171
BstMCI CGRYCG 1 cut(s) 280
BstMWI GCNNNNNNNGC 3 cut(s) 156, 770, 1123
BstPAI GACNNNNGTC 1 cut(s) 62
BstPI GGTNACC 2 cut(s) 374, 530
BstSFI CTRYAG 1 cut(s) 157
BstSLI GKGCMC 1 cut(s) 74
BstV1I GCAGC 5 cut(s) 33, 36, 112, 159, 171
BstV2I GAAGAC 3 cut(s) 310, 639, 825
BstX2I RGATCY 1 cut(s) 365
BstYI RGATCY 1 cut(s) 365
BsuI GTATCC 1 cut(s) 60
BsuRI GGCC 3 cut(s) 250, 433, 1024
BtgI CCRYGG 1 cut(s) 131
BtgZI GCGATG 1 cut(s) 190
BtsCI GGATG 4 cut(s) 543, 691, 726, 889
BtsIMutI CAGTG 2 cut(s) 202, 376
BveI ACCTGC 1 cut(s) 518
Cac8I GCNNGC 1 cut(s) 1022
CfoI GCGC 3 cut(s) 221, 764, 930
Cfr13I GGNCC 3 cut(s) 57, 432, 507
CseI GACGC 1 cut(s) 336
Csp6I GTAC 2 cut(s) 648, 1113
CviAII CATG 5 cut(s) 35, 67, 536, 812, 874
CviJI RGCY 8 cut(s) 27, 150, 250, 433, 604, 899, 1024, 1228
CviKI_1 RGCY 8 cut(s) 27, 150, 250, 433, 604, 899, 1024, 1228
CviQI GTAC 2 cut(s) 648, 1113
DdeI CTNAG 3 cut(s) 175, 491, 1093
DinI GGCGCC 1 cut(s) 220
DpnI GATC 4 cut(s) 367, 599, 660, 1173
DpnII GATC 4 cut(s) 365, 597, 658, 1171
DraI TTTAAA 1 cut(s) 981
DrdI GACNNNNNNGTC 1 cut(s) 312
DseDI GACNNNNNNGTC 1 cut(s) 312
EaeI YGGCCR 1 cut(s) 248
Eam1104I CTCTTC 1 cut(s) 616
EarI CTCTTC 1 cut(s) 616
Eco130I CCWWGG 3 cut(s) 53, 379, 879
Eco32I GATATC 1 cut(s) 988
Eco47I GGWCC 2 cut(s) 57, 507
Eco57I CTGAAG 1 cut(s) 597
Eco91I GGTNACC 2 cut(s) 374, 530
EcoNI CCTNNNNNAGG 1 cut(s) 1029
EcoO109I RGGNCCY 1 cut(s) 432
EcoO65I GGTNACC 2 cut(s) 374, 530
EcoRV GATATC 1 cut(s) 988
EcoT14I CCWWGG 3 cut(s) 53, 379, 879
EgeI GGCGCC 1 cut(s) 220
EheI GGCGCC 1 cut(s) 220
ErhI CCWWGG 3 cut(s) 53, 379, 879
FaeI CATG 5 cut(s) 38, 70, 539, 815, 877
FaqI GGGAC 1 cut(s) 696
FatI CATG 5 cut(s) 34, 66, 535, 811, 873
FauNDI CATATG 1 cut(s) 703
FbaI TGATCA 1 cut(s) 1171
Fnu4HI GCNGC 6 cut(s) 22, 25, 126, 148, 160, 1226
FokI GGATG 4 cut(s) 530, 698, 713, 896
Fsp4HI GCNGC 6 cut(s) 22, 25, 126, 148, 160, 1226
FspBI CTAG 1 cut(s) 1029
GlaI GCGC 3 cut(s) 220, 763, 929
GluI GCNGC 6 cut(s) 22, 25, 126, 148, 160, 1226
HaeII RGCGCY 2 cut(s) 222, 931
HaeIII GGCC 3 cut(s) 250, 433, 1024
HapII CCGG 1 cut(s) 1103
HgaI GACGC 1 cut(s) 336
HhaI GCGC 3 cut(s) 221, 764, 930
Hin1I GRCGYC 1 cut(s) 219
Hin1II CATG 5 cut(s) 38, 70, 539, 815, 877
Hin6I GCGC 3 cut(s) 219, 762, 928
HinP1I GCGC 3 cut(s) 219, 762, 928
HincII GTYRAC 1 cut(s) 294
HindII GTYRAC 1 cut(s) 294
HinfI GANTC 3 cut(s) 608, 1091, 1131
HpaII CCGG 1 cut(s) 1103
HphI GGTGA 8 cut(s) 100, 368, 386, 489, 506, 665, 901, 1055
Hpy166II GTNNAC 4 cut(s) 72, 294, 497, 909
Hpy188I TCNGA 8 cut(s) 168, 241, 555, 616, 805, 1090, 1171, 1180
Hpy188III TCNNGA 2 cut(s) 177, 1103
Hpy8I GTNNAC 4 cut(s) 72, 294, 497, 909
Hpy99I CGWCG 1 cut(s) 592
HpyAV CCTTC 4 cut(s) 279, 496, 521, 1034
HpyCH4III ACNGT 3 cut(s) 197, 278, 638
HpyCH4IV ACGT 2 cut(s) 63, 74
HpyCH4V TGCA 6 cut(s) 72, 159, 527, 627, 773, 815
HpyF10VI GCNNNNNNNGC 3 cut(s) 156, 770, 1123
HpyF3I CTNAG 3 cut(s) 175, 491, 1093
HpySE526I ACGT 2 cut(s) 63, 74
Hsp92I GRCGYC 1 cut(s) 219
Hsp92II CATG 5 cut(s) 38, 70, 539, 815, 877
HspAI GCGC 3 cut(s) 219, 762, 928
KasI GGCGCC 1 cut(s) 218
Kpn2I TCCGGA 1 cut(s) 1102
Ksp22I TGATCA 1 cut(s) 1171
Kzo9I GATC 4 cut(s) 365, 597, 658, 1171
LmnI GCTCC 4 cut(s) 133, 179, 571, 925
Lsp1109I GCAGC 5 cut(s) 33, 36, 112, 159, 171
LweI GCATC 1 cut(s) 396
MaeI CTAG 1 cut(s) 1029
MaeII ACGT 2 cut(s) 63, 74
MaeIII GTNAC 4 cut(s) 374, 530, 538, 1157
MalI GATC 4 cut(s) 367, 599, 660, 1173
MboI GATC 4 cut(s) 365, 597, 658, 1171
MboII GAAGA 5 cut(s) 315, 603, 644, 830, 1188
MfeI CAATTG 2 cut(s) 252, 774
MflI RGATCY 1 cut(s) 365
MhlI GDGCHC 1 cut(s) 74
MlsI TGGCCA 1 cut(s) 250
MluNI TGGCCA 1 cut(s) 250
Mly113I GGCGCC 1 cut(s) 219
MlyI GAGTC 2 cut(s) 602, 1085
MmeI TCCRAC 1 cut(s) 423
Mox20I TGGCCA 1 cut(s) 250
MroI TCCGGA 1 cut(s) 1102
MroXI GAANNNNTTC 1 cut(s) 1050
MscI TGGCCA 1 cut(s) 250
MseI TTAA 1 cut(s) 980
MslI CAYNNNNRTG 1 cut(s) 1179
Msp20I TGGCCA 1 cut(s) 250
MspI CCGG 1 cut(s) 1103
MunI CAATTG 2 cut(s) 252, 774
MwoI GCNNNNNNNGC 3 cut(s) 156, 770, 1123
NarI GGCGCC 1 cut(s) 219
NdeI CATATG 1 cut(s) 703
NdeII GATC 4 cut(s) 365, 597, 658, 1171
NlaIII CATG 5 cut(s) 38, 70, 539, 815, 877
NlaIV GGNNCC 5 cut(s) 220, 404, 434, 509, 838
NmeAIII GCCGAG 1 cut(s) 96
NmuCI GTSAC 1 cut(s) 374
PdmI GAANNNNTTC 1 cut(s) 1050
PfeI GAWTC 1 cut(s) 1131
PflMI CCANNNNNTGG 1 cut(s) 442
PkrI GCNGC 6 cut(s) 23, 26, 127, 149, 161, 1227
PleI GAGTC 2 cut(s) 602, 1085
PluTI GGCGCC 1 cut(s) 222
PpsI GAGTC 2 cut(s) 602, 1085
PshAI GACNNNNGTC 1 cut(s) 62
PspEI GGTNACC 2 cut(s) 374, 530
PspN4I GGNNCC 5 cut(s) 220, 404, 434, 509, 838
PspPI GGNCC 3 cut(s) 57, 432, 507
PstI CTGCAG 1 cut(s) 161
PsuI RGATCY 1 cut(s) 365
RsaI GTAC 2 cut(s) 649, 1114
RsaNI GTAC 2 cut(s) 648, 1113
RseI CAYNNNNRTG 1 cut(s) 1179
SaqAI TTAA 1 cut(s) 980
SatI GCNGC 6 cut(s) 22, 25, 126, 148, 160, 1226
Sau3AI GATC 4 cut(s) 365, 597, 658, 1171
Sau96I GGNCC 3 cut(s) 57, 432, 507
ScaI AGTACT 1 cut(s) 649
SchI GAGTC 2 cut(s) 602, 1085
SduI GDGCHC 1 cut(s) 74
SfaNI GCATC 1 cut(s) 396
SfcI CTRYAG 1 cut(s) 157
SfoI GGCGCC 1 cut(s) 220
SinI GGWCC 2 cut(s) 57, 507
SmiMI CAYNNNNRTG 1 cut(s) 1179
SmlI CTYRAG 2 cut(s) 572, 1121
SmoI CTYRAG 2 cut(s) 572, 1121
SsiI CCGC 1 cut(s) 1225
SspDI GGCGCC 1 cut(s) 218
SspMI CTAG 1 cut(s) 1029
StyI CCWWGG 3 cut(s) 53, 379, 879
TaaI ACNGT 3 cut(s) 197, 278, 638
TaiI ACGT 2 cut(s) 66, 77
TaqI TCGA 4 cut(s) 187, 280, 310, 1207
TatI WGTACW 1 cut(s) 647
TauI GCSGC 1 cut(s) 1228
TfiI GAWTC 1 cut(s) 1131
Tru1I TTAA 1 cut(s) 980
Tru9I TTAA 1 cut(s) 980
TscAI CASTG 2 cut(s) 202, 376
TseFI GTSAC 1 cut(s) 374
TseI GCWGC 5 cut(s) 21, 24, 125, 147, 159
Tsp45I GTSAC 1 cut(s) 374
TspDTI ATGAA 2 cut(s) 690, 800
TspGWI ACGGA 2 cut(s) 120, 353
TspRI CASTG 2 cut(s) 202, 376
Van91I CCANNNNNTGG 1 cut(s) 442
VneI GTGCAC 1 cut(s) 70
VpaK11BI GGWCC 2 cut(s) 57, 507
XagI CCTNNNNNAGG 1 cut(s) 1029
XapI RAATTY 3 cut(s) 212, 865, 1048
XmnI GAANNNNTTC 1 cut(s) 1050
XspI CTAG 1 cut(s) 1029
ZrmI AGTACT 1 cut(s) 649
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.