pycom2247g00040

mitotic sister chromatid biorientation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
tig00002247
OG0017764
Physical Location & Seq
Forward (+)
18669 .. 19377
709 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 507 bp
ATGCTCTCACTCGAACCCTTCTCAGAAGATCAAGGTCGGTCGGCGGTGCAACCCTCTGGGGATCCCGCCAGTCAGCTTCCTTGCACTCCTTGGTCCGTGTTTCAAGACGGGCCGAATGGGAGCTCGCTGGCCGACGCCGGGAGCGCGCAGTTGCCGAAGCACGCCGGTACGGCGCGCGCTGCCCGCCACGATCGCGTCGACGGCGTCTCCGCGGCATATCAACAGCCCGGCTTGGCCGCCGCCGCAATCCGCGTCGGTCCACGCCCCGAGCCGATCGGCGGACCGGCTTTCGACCGTTCCACATCCGACCGGGCGCATCGCCAGCCCCCATCCGCTTCCCTCCCGACAATTTCAAGGTCCGGCACGACGGGCTCTCACCCTCTCCGCGCCACCTTCCAGTGGACTTGGCCCGGTCCGCCGCTGAGGACGCTTCTCCAGACTACAATTCGAACGCCGACGGCGCCCGATTCTCAAGCTGGCTGTTCCCGGTTCGCTCGCCGTTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

169

Amino Acids

17.72

Weight (kDa)

10.21

Isoelectric Point (pI)

53.76

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 460
AccI GTMKAC 1 cut(s) 198
AccII CGCG 7 cut(s) 146, 175, 177, 195, 212, 252, 387
AclWI GGATC 2 cut(s) 56, 69
AcoI YGGCCR 2 cut(s) 129, 234
AcyI GRCGYC 3 cut(s) 135, 204, 461
AfaI GTAC 1 cut(s) 169
AfiI CCNNNNNNNGG 3 cut(s) 138, 278, 399
AgsI TTSAA 2 cut(s) 104, 354
AluBI AGCT 3 cut(s) 76, 123, 476
AluI AGCT 3 cut(s) 76, 123, 476
Alw21I GWGCWC 1 cut(s) 125
Alw26I GTCTC 1 cut(s) 211
AlwI GGATC 2 cut(s) 56, 69
Ama87I CYCGRG 1 cut(s) 266
AoxI GGCC 4 cut(s) 110, 129, 234, 407
ApeKI GCWGC 1 cut(s) 179
AspLEI GCGC 8 cut(s) 146, 148, 175, 177, 179, 316, 389, 463
AspS9I GGNCC 7 cut(s) 93, 110, 257, 281, 357, 408, 413
AsuC2I CCSGG 5 cut(s) 139, 228, 311, 411, 487
AsuHPI GGTGA 1 cut(s) 368
AsuII TTCGAA 1 cut(s) 448
AvaI CYCGRG 1 cut(s) 266
AvaII GGWCC 5 cut(s) 93, 257, 281, 357, 413
BamHI GGATCC 1 cut(s) 61
BanI GGYRCC 1 cut(s) 460
BanII GRGCYC 2 cut(s) 125, 374
Bbv12I GWGCWC 1 cut(s) 125
BbvCI CCTCAGC 1 cut(s) 422
BbvI GCAGC 1 cut(s) 166
BccI CCATC 1 cut(s) 337
BceAI ACGGC 4 cut(s) 186, 217, 474, 483
BcnI CCSGG 5 cut(s) 139, 228, 311, 411, 487
BcoDI GTCTC 1 cut(s) 211
BfaI CTAG 1 cut(s) 505
BfoI RGCGCY 1 cut(s) 464
BglI GCCNNNNNGGC 1 cut(s) 170
BisI GCNGC 6 cut(s) 180, 213, 237, 240, 243, 419
BlsI GCNGC 6 cut(s) 181, 214, 238, 241, 244, 420
Bme1390I CCNGG 5 cut(s) 139, 228, 311, 411, 487
Bme18I GGWCC 5 cut(s) 93, 257, 281, 357, 413
BmeT110I CYCGRG 1 cut(s) 266
BmgT120I GGNCC 7 cut(s) 93, 110, 257, 281, 357, 408, 413
BmiI GGNNCC 2 cut(s) 63, 462
BmrFI CCNGG 5 cut(s) 139, 228, 311, 411, 487
BmsI GCATC 1 cut(s) 325
BpmI CTGGAG 1 cut(s) 419
Bpu10I CCTNAGC 1 cut(s) 422
Bpu14I TTCGAA 1 cut(s) 448
BpuEI CTTGAG 1 cut(s) 456
BpuMI CCSGG 5 cut(s) 139, 228, 311, 411, 487
BsaHI GRCGYC 3 cut(s) 135, 204, 461
BsaJI CCNNGG 2 cut(s) 89, 210
BsaXI ACNNNNNCTCC 2 cut(s) 191, 221
Bsc4I CCNNNNNNNGG 3 cut(s) 138, 278, 399
Bse118I RCCGGY 2 cut(s) 164, 283
Bse1I ACTGG 2 cut(s) 69, 397
BseDI CCNNGG 2 cut(s) 89, 210
BseGI GGATG 2 cut(s) 302, 329
BseLI CCNNNNNNNGG 3 cut(s) 138, 278, 399
BseMII CTCAG 2 cut(s) 36, 413
BseNI ACTGG 2 cut(s) 69, 397
BsePI GCGCGC 3 cut(s) 144, 173, 175
BseXI GCAGC 1 cut(s) 166
Bsh1236I CGCG 7 cut(s) 146, 175, 177, 195, 212, 252, 387
Bsh1285I CGRYCG 5 cut(s) 41, 193, 276, 295, 310
BshFI GGCC 4 cut(s) 112, 131, 236, 409
BshNI GGYRCC 1 cut(s) 460
BsiEI CGRYCG 5 cut(s) 41, 193, 276, 295, 310
BsiHKAI GWGCWC 1 cut(s) 125
BsiHKCI CYCGRG 1 cut(s) 266
BsiSI CCGG 8 cut(s) 138, 165, 228, 284, 310, 360, 411, 487
BslI CCNNNNNNNGG 3 cut(s) 138, 278, 399
BsmAI GTCTC 1 cut(s) 211
BsmBI CGTCTC 1 cut(s) 211
BsnI GGCC 4 cut(s) 112, 131, 236, 409
BsoBI CYCGRG 1 cut(s) 266
Bsp119I TTCGAA 1 cut(s) 448
Bsp1286I GDGCHC 2 cut(s) 125, 374
Bsp143I GATC 4 cut(s) 28, 61, 190, 273
BspANI GGCC 4 cut(s) 112, 131, 236, 409
BspCNI CTCAG 2 cut(s) 35, 414
BspFNI CGCG 7 cut(s) 146, 175, 177, 195, 212, 252, 387
BspLI GGNNCC 2 cut(s) 63, 462
BspPI GGATC 2 cut(s) 56, 69
BspT104I TTCGAA 1 cut(s) 448
BspT107I GGYRCC 1 cut(s) 460
BsrFI RCCGGY 2 cut(s) 164, 283
BsrI ACTGG 2 cut(s) 69, 397
BssAI RCCGGY 2 cut(s) 164, 283
BssECI CCNNGG 2 cut(s) 89, 210
BssHII GCGCGC 3 cut(s) 144, 173, 175
BssMI GATC 4 cut(s) 28, 61, 190, 273
BssNI GRCGYC 3 cut(s) 135, 204, 461
BssT1I CCWWGG 1 cut(s) 89
Bst4CI ACNGT 1 cut(s) 296
BstACI GRCGYC 3 cut(s) 135, 204, 461
BstBI TTCGAA 1 cut(s) 448
BstDEI CTNAG 2 cut(s) 22, 422
BstDSI CCRYGG 1 cut(s) 210
BstF5I GGATG 2 cut(s) 302, 329
BstFNI CGCG 7 cut(s) 146, 175, 177, 195, 212, 252, 387
BstH2I RGCGCY 1 cut(s) 464
BstHHI GCGC 8 cut(s) 146, 148, 175, 177, 179, 316, 389, 463
BstKTI GATC 4 cut(s) 31, 64, 193, 276
BstMAI GTCTC 1 cut(s) 211
BstMBI GATC 4 cut(s) 28, 61, 190, 273
BstMCI CGRYCG 5 cut(s) 41, 193, 276, 295, 310
BstSCI CCNGG 5 cut(s) 137, 226, 309, 409, 485
BstUI CGCG 7 cut(s) 146, 175, 177, 195, 212, 252, 387
BstV1I GCAGC 1 cut(s) 166
BstX2I RGATCY 1 cut(s) 61
BstYI RGATCY 1 cut(s) 61
BsuRI GGCC 4 cut(s) 112, 131, 236, 409
BtgI CCRYGG 1 cut(s) 210
BtgZI GCGATG 1 cut(s) 302
BtsCI GGATG 2 cut(s) 302, 329
BtsIMutI CAGTG 1 cut(s) 404
CfoI GCGC 8 cut(s) 146, 148, 175, 177, 179, 316, 389, 463
Cfr10I RCCGGY 2 cut(s) 164, 283
Cfr13I GGNCC 7 cut(s) 93, 110, 257, 281, 357, 408, 413
Cfr42I CCGCGG 1 cut(s) 213
CpoI CGGWCCG 2 cut(s) 281, 413
CseI GACGC 5 cut(s) 143, 184, 193, 241, 436
Csp6I GTAC 1 cut(s) 168
CspI CGGWCCG 2 cut(s) 281, 413
CviQI GTAC 1 cut(s) 168
DdeI CTNAG 2 cut(s) 22, 422
DinI GGCGCC 1 cut(s) 462
DpnI GATC 4 cut(s) 30, 63, 192, 275
DpnII GATC 4 cut(s) 28, 61, 190, 273
EaeI YGGCCR 2 cut(s) 129, 234
EciI GGCGGA 2 cut(s) 294, 405
Ecl136II GAGCTC 1 cut(s) 123
Eco130I CCWWGG 1 cut(s) 89
Eco24I GRGCYC 2 cut(s) 125, 374
Eco47I GGWCC 5 cut(s) 93, 257, 281, 357, 413
Eco53kI GAGCTC 1 cut(s) 123
Eco88I CYCGRG 1 cut(s) 266
EcoICRI GAGCTC 1 cut(s) 123
EcoT14I CCWWGG 1 cut(s) 89
EcoT38I GRGCYC 2 cut(s) 125, 374
EgeI GGCGCC 1 cut(s) 462
EheI GGCGCC 1 cut(s) 462
ErhI CCWWGG 1 cut(s) 89
Esp3I CGTCTC 1 cut(s) 211
FaiI YATR 1 cut(s) 217
FauI CCCGC 2 cut(s) 73, 191
FblI GTMKAC 1 cut(s) 198
Fnu4HI GCNGC 6 cut(s) 180, 213, 237, 240, 243, 419
FokI GGATG 2 cut(s) 289, 316
FriOI GRGCYC 2 cut(s) 125, 374
Fsp4HI GCNGC 6 cut(s) 180, 213, 237, 240, 243, 419
FspBI CTAG 1 cut(s) 505
GlaI GCGC 8 cut(s) 145, 147, 174, 176, 178, 315, 388, 462
GluI GCNGC 6 cut(s) 180, 213, 237, 240, 243, 419
GsuI CTGGAG 1 cut(s) 419
HaeII RGCGCY 1 cut(s) 464
HaeIII GGCC 4 cut(s) 112, 131, 236, 409
HapII CCGG 8 cut(s) 138, 165, 228, 284, 310, 360, 411, 487
HgaI GACGC 5 cut(s) 143, 184, 193, 241, 436
HhaI GCGC 8 cut(s) 146, 148, 175, 177, 179, 316, 389, 463
Hin1I GRCGYC 3 cut(s) 135, 204, 461
Hin6I GCGC 8 cut(s) 144, 146, 173, 175, 177, 314, 387, 461
HinP1I GCGC 8 cut(s) 144, 146, 173, 175, 177, 314, 387, 461
HincII GTYRAC 1 cut(s) 199
HindII GTYRAC 1 cut(s) 199
HinfI GANTC 1 cut(s) 467
HpaII CCGG 8 cut(s) 138, 165, 228, 284, 310, 360, 411, 487
HphI GGTGA 1 cut(s) 368
Hpy166II GTNNAC 3 cut(s) 199, 260, 402
Hpy188I TCNGA 2 cut(s) 25, 307
Hpy188III TCNNGA 3 cut(s) 104, 343, 436
Hpy8I GTNNAC 3 cut(s) 199, 260, 402
Hpy99I CGWCG 6 cut(s) 137, 200, 203, 257, 370, 460
HpyAV CCTTC 2 cut(s) 28, 403
HpyCH4III ACNGT 1 cut(s) 296
HpyCH4V TGCA 2 cut(s) 49, 84
HpyF3I CTNAG 2 cut(s) 22, 422
Hsp92I GRCGYC 3 cut(s) 135, 204, 461
HspAI GCGC 8 cut(s) 144, 146, 173, 175, 177, 314, 387, 461
KasI GGCGCC 1 cut(s) 460
KspI CCGCGG 1 cut(s) 213
Kzo9I GATC 4 cut(s) 28, 61, 190, 273
LmnI GCTCC 2 cut(s) 120, 141
Lsp1109I GCAGC 1 cut(s) 166
LweI GCATC 1 cut(s) 325
MaeI CTAG 1 cut(s) 505
MaeIII GTNAC 1 cut(s) 500
MalI GATC 4 cut(s) 30, 63, 192, 275
MboI GATC 4 cut(s) 28, 61, 190, 273
MboII GAAGA 1 cut(s) 38
MflI RGATCY 1 cut(s) 61
MhlI GDGCHC 2 cut(s) 125, 374
MluCI AATT 2 cut(s) 348, 444
Mly113I GGCGCC 1 cut(s) 461
MmeI TCCRAC 1 cut(s) 330
MnlI CCTC 4 cut(s) 64, 350, 390, 417
MspA1I CMGCKG 2 cut(s) 212, 421
MspI CCGG 8 cut(s) 138, 165, 228, 284, 310, 360, 411, 487
MspR9I CCNGG 5 cut(s) 139, 228, 311, 411, 487
MvnI CGCG 7 cut(s) 146, 175, 177, 195, 212, 252, 387
NarI GGCGCC 1 cut(s) 461
NciI CCSGG 5 cut(s) 139, 228, 311, 411, 487
NdeII GATC 4 cut(s) 28, 61, 190, 273
NlaIV GGNNCC 2 cut(s) 63, 462
NspV TTCGAA 1 cut(s) 448
PauI GCGCGC 3 cut(s) 144, 173, 175
PcsI WCGNNNNNNNCGW 1 cut(s) 195
PfeI GAWTC 1 cut(s) 467
PflFI GACNNNGTC 1 cut(s) 203
PkrI GCNGC 6 cut(s) 181, 214, 238, 241, 244, 420
Ple19I CGATCG 2 cut(s) 193, 276
PluTI GGCGCC 1 cut(s) 464
Psp124BI GAGCTC 1 cut(s) 125
PspN4I GGNNCC 2 cut(s) 63, 462
PspPI GGNCC 7 cut(s) 93, 110, 257, 281, 357, 408, 413
PsuI RGATCY 1 cut(s) 61
PsyI GACNNNGTC 1 cut(s) 203
PteI GCGCGC 3 cut(s) 144, 173, 175
PvuI CGATCG 2 cut(s) 193, 276
RsaI GTAC 1 cut(s) 169
RsaNI GTAC 1 cut(s) 168
Rsr2I CGGWCCG 2 cut(s) 281, 413
RsrII CGGWCCG 2 cut(s) 281, 413
SacI GAGCTC 1 cut(s) 125
SacII CCGCGG 1 cut(s) 213
SalI GTCGAC 1 cut(s) 197
SatI GCNGC 6 cut(s) 180, 213, 237, 240, 243, 419
Sau3AI GATC 4 cut(s) 28, 61, 190, 273
Sau96I GGNCC 7 cut(s) 93, 110, 257, 281, 357, 408, 413
ScrFI CCNGG 5 cut(s) 139, 228, 311, 411, 487
SduI GDGCHC 2 cut(s) 125, 374
SetI ASST 6 cut(s) 37, 78, 125, 359, 395, 478
SfaNI GCATC 1 cut(s) 325
SfoI GGCGCC 1 cut(s) 462
Sfr303I CCGCGG 1 cut(s) 213
SfuI TTCGAA 1 cut(s) 448
SgrBI CCGCGG 1 cut(s) 213
SgrDI CGTCGACG 1 cut(s) 197
SinI GGWCC 5 cut(s) 93, 257, 281, 357, 413
SmlI CTYRAG 1 cut(s) 471
SmoI CTYRAG 1 cut(s) 471
Sse9I AATT 2 cut(s) 348, 444
SspDI GGCGCC 1 cut(s) 460
SspMI CTAG 1 cut(s) 505
SstI GAGCTC 1 cut(s) 125
StyD4I CCNGG 5 cut(s) 137, 226, 309, 409, 485
StyI CCWWGG 1 cut(s) 89
TaaI ACNGT 1 cut(s) 296
TaqI TCGA 4 cut(s) 12, 198, 291, 448
TaqII GACCGA 2 cut(s) 27, 245
TasI AATT 2 cut(s) 348, 444
TauI GCSGC 5 cut(s) 215, 239, 242, 245, 421
TfiI GAWTC 1 cut(s) 467
TscAI CASTG 1 cut(s) 404
TseI GCWGC 1 cut(s) 179
TspGWI ACGGA 1 cut(s) 85
TspRI CASTG 1 cut(s) 404
Tth111I GACNNNGTC 1 cut(s) 203
VpaK11BI GGWCC 5 cut(s) 93, 257, 281, 357, 413
XmiI GTMKAC 1 cut(s) 198
XspI CTAG 1 cut(s) 505
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.