Rroxscaffold_22G00439880

mitotic sister chromatid biorientation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000022
OG0017764
Physical Location & Seq
Reverse (-)
84287 .. 84541
255 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_22G00439880.1

Sequence Viewer

Length: 255 bp
ATGGGGAGCCCGCAGGCCGTTACCAGGAGCACGCAGATGCCGAAGCACGCCGAGACGGCGCATGCCGCCTACCATGATCGCGTCGACGACGTCTCCACGGGCATATCAACAGCCCGGGCTTTGGCCGCCGCCGCAATCCGTAACGGTCCACGCCCCGAGTCGAGTGGCGGACCGGCTTGTGACCGTTCCACATCCGACCGAGGCGCATCGCCGCCCCCATCCGCTTCCCTCCCGACAATTTCAAGCACTCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

84

Amino Acids

8.51

Weight (kDa)

8.02

Isoelectric Point (pI)

58.83

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 93
AccI GTMKAC 1 cut(s) 84
AccII CGCG 1 cut(s) 81
AciI CCGC 8 cut(s) 11, 66, 126, 129, 132, 168, 212, 222
AcoI YGGCCR 1 cut(s) 123
AcyI GRCGYC 1 cut(s) 90
AfiI CCNNNNNNNGG 2 cut(s) 24, 121
AgsI TTSAA 1 cut(s) 243
AjnI CCWGG 1 cut(s) 23
Alw21I GWGCWC 1 cut(s) 32
Alw26I GTCTC 2 cut(s) 47, 97
Ama87I CYCGRG 2 cut(s) 114, 155
AoxI GGCC 2 cut(s) 15, 123
AspLEI GCGC 2 cut(s) 61, 206
AspS9I GGNCC 2 cut(s) 146, 170
AsuC2I CCSGG 2 cut(s) 115, 116
AvaI CYCGRG 2 cut(s) 114, 155
AvaII GGWCC 2 cut(s) 146, 170
BanII GRGCYC 1 cut(s) 11
Bbv12I GWGCWC 1 cut(s) 32
BccI CCATC 1 cut(s) 226
BceAI ACGGC 2 cut(s) 2, 72
BciT130I CCWGG 1 cut(s) 25
BcnI CCSGG 2 cut(s) 115, 116
BcoDI GTCTC 2 cut(s) 47, 97
BglI GCCNNNNNGGC 1 cut(s) 56
BisI GCNGC 5 cut(s) 66, 126, 129, 132, 212
BlsI GCNGC 5 cut(s) 67, 127, 130, 133, 213
Bme1390I CCNGG 3 cut(s) 25, 115, 116
Bme18I GGWCC 2 cut(s) 146, 170
BmeT110I CYCGRG 2 cut(s) 114, 155
BmgT120I GGNCC 2 cut(s) 146, 170
BmiI GGNNCC 1 cut(s) 8
BmrFI CCNGG 3 cut(s) 25, 115, 116
BmsI GCATC 2 cut(s) 27, 215
BpuMI CCSGG 2 cut(s) 115, 116
BsaHI GRCGYC 1 cut(s) 90
BsaJI CCNNGG 3 cut(s) 96, 114, 199
BsaXI ACNNNNNCTCC 2 cut(s) 77, 107
Bsc4I CCNNNNNNNGG 2 cut(s) 24, 121
Bse118I RCCGGY 1 cut(s) 172
BseBI CCWGG 1 cut(s) 25
BseDI CCNNGG 3 cut(s) 96, 114, 199
BseGI GGATG 2 cut(s) 191, 218
BseLI CCNNNNNNNGG 2 cut(s) 24, 121
Bsh1236I CGCG 1 cut(s) 81
Bsh1285I CGRYCG 1 cut(s) 199
BshFI GGCC 2 cut(s) 17, 125
BsiEI CGRYCG 1 cut(s) 199
BsiHKAI GWGCWC 1 cut(s) 32
BsiHKCI CYCGRG 2 cut(s) 114, 155
BsiSI CCGG 2 cut(s) 115, 173
BslI CCNNNNNNNGG 2 cut(s) 24, 121
BsmAI GTCTC 2 cut(s) 47, 97
BsmBI CGTCTC 2 cut(s) 47, 97
BsnI GGCC 2 cut(s) 17, 125
BsoBI CYCGRG 2 cut(s) 114, 155
Bsp1286I GDGCHC 2 cut(s) 11, 32
Bsp143I GATC 1 cut(s) 76
BspACI CCGC 8 cut(s) 11, 66, 126, 129, 132, 168, 212, 222
BspANI GGCC 2 cut(s) 17, 125
BspFNI CGCG 1 cut(s) 81
BspLI GGNNCC 1 cut(s) 8
BsrFI RCCGGY 1 cut(s) 172
BssAI RCCGGY 1 cut(s) 172
BssECI CCNNGG 3 cut(s) 96, 114, 199
BssMI GATC 1 cut(s) 76
BssNI GRCGYC 1 cut(s) 90
Bst2UI CCWGG 1 cut(s) 25
Bst4CI ACNGT 2 cut(s) 146, 185
BstACI GRCGYC 1 cut(s) 90
BstC8I GCNNGC 5 cut(s) 11, 15, 32, 48, 63
BstDSI CCRYGG 1 cut(s) 96
BstF5I GGATG 2 cut(s) 191, 218
BstFNI CGCG 1 cut(s) 81
BstHHI GCGC 2 cut(s) 61, 206
BstKTI GATC 1 cut(s) 79
BstMAI GTCTC 2 cut(s) 47, 97
BstMBI GATC 1 cut(s) 76
BstMCI CGRYCG 1 cut(s) 199
BstMWI GCNNNNNNNGC 4 cut(s) 56, 65, 125, 131
BstNI CCWGG 1 cut(s) 25
BstNSI RCATGY 1 cut(s) 65
BstSCI CCNGG 3 cut(s) 23, 113, 114
BstUI CGCG 1 cut(s) 81
BsuRI GGCC 2 cut(s) 17, 125
BtgI CCRYGG 1 cut(s) 96
BtgZI GCGATG 1 cut(s) 192
BtsCI GGATG 2 cut(s) 191, 218
Cac8I GCNNGC 5 cut(s) 11, 15, 32, 48, 63
CfoI GCGC 2 cut(s) 61, 206
Cfr10I RCCGGY 1 cut(s) 172
Cfr13I GGNCC 2 cut(s) 146, 170
Cfr9I CCCGGG 1 cut(s) 114
CpoI CGGWCCG 1 cut(s) 170
CseI GACGC 1 cut(s) 70
CspI CGGWCCG 1 cut(s) 170
CviAII CATG 2 cut(s) 62, 74
CviJI RGCY 6 cut(s) 9, 17, 113, 119, 125, 176
CviKI_1 RGCY 6 cut(s) 9, 17, 113, 119, 125, 176
DpnI GATC 1 cut(s) 78
DpnII GATC 1 cut(s) 76
EaeI YGGCCR 1 cut(s) 123
EciI GGCGGA 1 cut(s) 183
Eco24I GRGCYC 1 cut(s) 11
Eco47I GGWCC 2 cut(s) 146, 170
Eco88I CYCGRG 2 cut(s) 114, 155
EcoRII CCWGG 1 cut(s) 23
EcoT38I GRGCYC 1 cut(s) 11
Esp3I CGTCTC 2 cut(s) 47, 97
FaeI CATG 2 cut(s) 65, 77
FaiI YATR 3 cut(s) 63, 75, 104
FatI CATG 2 cut(s) 61, 73
FauI CCCGC 1 cut(s) 18
FblI GTMKAC 1 cut(s) 84
Fnu4HI GCNGC 5 cut(s) 66, 126, 129, 132, 212
FokI GGATG 2 cut(s) 178, 205
FriOI GRGCYC 1 cut(s) 11
Fsp4HI GCNGC 5 cut(s) 66, 126, 129, 132, 212
GlaI GCGC 2 cut(s) 60, 205
GluI GCNGC 5 cut(s) 66, 126, 129, 132, 212
HaeIII GGCC 2 cut(s) 17, 125
HapII CCGG 2 cut(s) 115, 173
HgaI GACGC 1 cut(s) 70
HhaI GCGC 2 cut(s) 61, 206
Hin1I GRCGYC 1 cut(s) 90
Hin1II CATG 2 cut(s) 65, 77
Hin6I GCGC 2 cut(s) 59, 204
HinP1I GCGC 2 cut(s) 59, 204
HincII GTYRAC 1 cut(s) 85
HindII GTYRAC 1 cut(s) 85
HinfI GANTC 1 cut(s) 158
HpaII CCGG 2 cut(s) 115, 173
Hpy166II GTNNAC 2 cut(s) 85, 149
Hpy188I TCNGA 1 cut(s) 196
Hpy188III TCNNGA 1 cut(s) 232
Hpy8I GTNNAC 2 cut(s) 85, 149
Hpy99I CGWCG 3 cut(s) 86, 89, 92
HpyCH4III ACNGT 2 cut(s) 146, 185
HpyCH4IV ACGT 1 cut(s) 90
HpyF10VI GCNNNNNNNGC 4 cut(s) 56, 65, 125, 131
HpySE526I ACGT 1 cut(s) 90
Hsp92I GRCGYC 1 cut(s) 90
Hsp92II CATG 2 cut(s) 65, 77
HspAI GCGC 2 cut(s) 59, 204
Kzo9I GATC 1 cut(s) 76
LmnI GCTCC 2 cut(s) 6, 27
LpnPI CCDG 4 cut(s) 10, 37, 128, 186
LweI GCATC 2 cut(s) 27, 215
MaeII ACGT 1 cut(s) 90
MaeIII GTNAC 3 cut(s) 19, 140, 179
MalI GATC 1 cut(s) 78
MboI GATC 1 cut(s) 76
MhlI GDGCHC 2 cut(s) 11, 32
MluCI AATT 1 cut(s) 237
MlyI GAGTC 1 cut(s) 167
MmeI TCCRAC 1 cut(s) 219
MnlI CCTC 2 cut(s) 194, 239
MslI CAYNNNNRTG 1 cut(s) 35
MspI CCGG 2 cut(s) 115, 173
MspR9I CCNGG 3 cut(s) 25, 115, 116
MvaI CCWGG 1 cut(s) 25
MvnI CGCG 1 cut(s) 81
MwoI GCNNNNNNNGC 4 cut(s) 56, 65, 125, 131
NciI CCSGG 2 cut(s) 115, 116
NdeII GATC 1 cut(s) 76
NlaIII CATG 2 cut(s) 65, 77
NlaIV GGNNCC 1 cut(s) 8
NmeAIII GCCGAG 1 cut(s) 76
NmuCI GTSAC 1 cut(s) 179
NspI RCATGY 1 cut(s) 65
PaeI GCATGC 1 cut(s) 65
PcsI WCGNNNNNNNCGW 1 cut(s) 38
PflFI GACNNNGTC 1 cut(s) 89
PkrI GCNGC 5 cut(s) 67, 127, 130, 133, 213
PleI GAGTC 1 cut(s) 166
PpsI GAGTC 1 cut(s) 166
Psp6I CCWGG 1 cut(s) 23
PspGI CCWGG 1 cut(s) 23
PspN4I GGNNCC 1 cut(s) 8
PspPI GGNCC 2 cut(s) 146, 170
PsyI GACNNNGTC 1 cut(s) 89
RseI CAYNNNNRTG 1 cut(s) 35
Rsr2I CGGWCCG 1 cut(s) 170
RsrII CGGWCCG 1 cut(s) 170
SalI GTCGAC 1 cut(s) 83
SatI GCNGC 5 cut(s) 66, 126, 129, 132, 212
Sau3AI GATC 1 cut(s) 76
Sau96I GGNCC 2 cut(s) 146, 170
SchI GAGTC 1 cut(s) 167
ScrFI CCNGG 3 cut(s) 25, 115, 116
SduI GDGCHC 2 cut(s) 11, 32
SetI ASST 1 cut(s) 93
SfaNI GCATC 2 cut(s) 27, 215
SgrDI CGTCGACG 1 cut(s) 83
SinI GGWCC 2 cut(s) 146, 170
SmaI CCCGGG 1 cut(s) 116
SmiMI CAYNNNNRTG 1 cut(s) 35
SphI GCATGC 1 cut(s) 65
SrfI GCCCGGGC 1 cut(s) 116
Sse9I AATT 1 cut(s) 237
SsiI CCGC 8 cut(s) 11, 66, 126, 129, 132, 168, 212, 222
StyD4I CCNGG 3 cut(s) 23, 113, 114
TaaI ACNGT 2 cut(s) 146, 185
TaiI ACGT 1 cut(s) 93
TaqI TCGA 2 cut(s) 84, 161
TaqII GACCGA 1 cut(s) 213
TasI AATT 1 cut(s) 237
TauI GCSGC 5 cut(s) 68, 128, 131, 134, 214
TseFI GTSAC 1 cut(s) 179
Tsp45I GTSAC 1 cut(s) 179
TspGWI ACGGA 1 cut(s) 128
TspMI CCCGGG 1 cut(s) 114
Tth111I GACNNNGTC 1 cut(s) 89
VpaK11BI GGWCC 2 cut(s) 146, 170
XceI RCATGY 1 cut(s) 65
XmaI CCCGGG 1 cut(s) 114
XmiI GTMKAC 1 cut(s) 84
ZraI GACGTC 1 cut(s) 91
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.